##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938960_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217314 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43216267704796 33.0 31.0 34.0 30.0 34.0 2 31.395630286129748 33.0 31.0 34.0 28.0 34.0 3 31.42004656855978 33.0 31.0 34.0 28.0 34.0 4 34.891645269057676 37.0 35.0 37.0 32.0 37.0 5 34.947849655337436 37.0 35.0 37.0 32.0 37.0 6 35.130911952290234 37.0 35.0 37.0 32.0 37.0 7 35.112100462924616 37.0 35.0 37.0 32.0 37.0 8 35.12767700194189 37.0 35.0 37.0 32.0 37.0 9 36.549739087219415 39.0 37.0 39.0 32.0 39.0 10-11 36.46094821318461 39.0 37.0 39.0 32.0 39.0 12-13 36.39843038184378 39.0 37.0 39.0 32.0 39.0 14-15 37.28923355145089 40.0 37.0 41.0 32.0 41.0 16-17 37.280499645674 40.0 37.0 41.0 32.0 41.0 18-19 37.250018406545365 40.0 36.5 41.0 32.0 41.0 20-21 37.082323274156295 39.5 36.0 41.0 31.5 41.0 22-23 37.201560875047164 40.0 36.5 41.0 32.0 41.0 24-25 37.02791122523169 39.5 36.0 41.0 31.5 41.0 26-27 36.81888649603799 39.0 36.0 41.0 30.5 41.0 28-29 36.73610765988386 39.0 36.0 41.0 30.5 41.0 30-31 36.68303698795292 39.0 35.5 41.0 30.0 41.0 32-33 36.356251783134084 39.0 35.0 41.0 30.0 41.0 34-35 35.89925867638532 38.5 35.0 41.0 29.5 41.0 36-37 35.50982449358992 38.5 35.0 40.0 28.0 41.0 38-39 35.233450214896415 38.0 35.0 40.0 27.0 41.0 40-41 35.26489779765684 38.0 35.0 40.0 27.0 41.0 42-43 34.9998343410917 38.0 35.0 40.0 25.0 41.0 44-45 34.841620880385065 38.0 35.0 40.0 24.0 41.0 46-47 34.464917124529485 38.0 34.5 40.0 23.0 41.0 48-49 34.126298811857495 38.0 34.0 40.0 21.0 41.0 50-51 32.85926815575619 37.0 32.0 39.0 16.5 40.5 52-53 33.15963766715444 37.5 33.0 39.5 18.0 40.0 54-55 33.759200971865596 38.0 34.0 40.0 16.0 41.0 56-57 33.51780143018858 38.0 33.5 40.0 11.0 41.0 58-59 33.32552205564299 38.0 33.0 40.0 9.0 41.0 60-61 32.757367219783355 38.0 32.0 40.0 7.5 41.0 62-63 32.4750269195726 37.0 32.0 40.0 2.0 41.0 64-65 32.00413917188952 36.5 31.5 39.5 2.0 40.5 66-67 31.353741590509586 36.0 31.0 39.0 2.0 40.0 68-69 30.821270143663085 35.5 30.5 38.5 2.0 39.5 70-71 30.049274321948886 35.0 29.0 37.0 2.0 39.0 72-73 29.478738139282328 34.5 29.0 37.0 2.0 39.0 74-75 29.011669749763016 34.0 29.0 36.0 2.0 37.5 76-77 28.59835307435324 34.0 29.0 35.5 2.0 37.0 78-79 28.025642618515143 34.0 27.0 35.0 2.0 36.5 80-81 27.435549481395583 34.0 26.0 35.0 2.0 36.0 82-83 26.96226888281473 33.5 25.5 35.0 2.0 36.0 84-85 26.50642618515144 33.0 25.0 35.0 2.0 35.0 86-87 26.23443036343724 33.0 24.5 35.0 2.0 35.0 88-89 25.62582254249611 33.0 20.0 35.0 2.0 35.0 90-91 25.017541437735257 32.0 18.0 35.0 2.0 35.0 92-93 24.414641946676237 32.0 8.0 35.0 2.0 35.0 94-95 23.774841933791656 32.0 2.0 35.0 2.0 35.0 96-97 22.987087808424675 31.0 2.0 34.0 2.0 35.0 98-99 22.287742621276124 31.0 2.0 34.0 2.0 35.0 100-101 19.81849305613076 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35470455447745763 1101 2 0.33645577651262926 1101 3 0.46203872329294526 1101 4 0.43364987033557867 1101 5 0.31766518515804876 1101 6 0.4720014822893006 1101 7 0.46797969762709357 1101 8 0.25070402091231614 1101 9 0.45498541045708407 1101 10-11 0.536640080333477 1101 12-13 0.5542061068716322 1101 14-15 0.03421308217522068 1101 16-17 0.408064937579681 1101 18-19 0.42131702211730016 1101 20-21 0.5277905630907114 1101 22-23 0.3101031695889276 1101 24-25 0.6152738248168532 1101 26-27 0.4097066143164838 1101 28-29 0.07793229364062881 1101 30-31 0.3140651863056618 1101 32-33 0.5518005612545451 1101 34-35 0.7885564918699117 1101 36-37 0.4879282095060731 1101 38-39 0.7592123839015699 1101 40-41 0.6542576948169696 1101 42-43 0.4694634485398339 1101 44-45 0.4890325989305566 1101 46-47 0.5059612120566968 1101 48-49 0.6421998894945986 1101 50-51 0.7043434808810005 1101 52-53 0.6265386712134813 1101 54-55 0.5518884426910446 1101 56-57 0.8440139882868607 1101 58-59 0.8237529007056708 1101 60-61 1.156598911497042 1101 62-63 0.6754135926537472 1101 64-65 0.8867051847094523 1101 66-67 0.912408135726011 1101 68-69 0.6598300510534649 1101 70-71 0.651581143167931 1101 72-73 0.3508546170038578 1101 74-75 0.5494443929299635 1101 76-77 0.14570162963986988 1101 78-79 0.382347655614101 1101 80-81 0.4603676054124257 1101 82-83 0.6849541078402659 1101 84-85 0.11685277160158236 1101 86-87 0.0918366522935905 1101 88-89 -0.36622757845555043 1101 90-91 -0.2909642369544052 1101 92-93 -0.12359686608114728 1101 94-95 -0.06758312892877782 1101 96-97 0.044121346009909246 1101 98-99 -0.420324071783746 1101 100-101 0.07925766744258667 1102 1 0.30967825127324744 1102 2 -0.11088999106269526 1102 3 -0.09243282142250209 1102 4 0.14627540835566322 1102 5 0.22106068970133208 1102 6 0.14667867023765524 1102 7 -0.029126949909972666 1102 8 0.0272224331552664 1102 9 0.27856264622944593 1102 10-11 0.05885433188775835 1102 12-13 0.2549952029978826 1102 14-15 0.17312986840190092 1102 16-17 0.1076703895165565 1102 18-19 0.34862453048649655 1102 20-21 -0.28283832260990494 1102 22-23 -0.3957911393541593 1102 24-25 -0.23191651473359798 1102 26-27 0.22230824033274388 1102 28-29 0.04762382879128069 1102 30-31 -0.19344318289567752 1102 32-33 -0.31637256165315364 1102 34-35 -0.3061230872788201 1102 36-37 -0.4359598823160198 1102 38-39 -0.3166604042380783 1102 40-41 -0.37782025831550214 1102 42-43 -0.3173490813501729 1102 44-45 -0.16626390991688567 1102 46-47 -0.641193259488027 1102 48-49 -0.38103062222229056 1102 50-51 -0.5772562321749461 1102 52-53 -0.5144106353384075 1102 54-55 -0.022669185238171963 1102 56-57 -0.30880762816460106 1102 58-59 -0.23191424420106443 1102 60-61 -0.3364068273838754 1102 62-63 -0.2405357138981472 1102 64-65 0.21694191354733405 1102 66-67 -0.1453393535135845 1102 68-69 0.06641780810750575 1102 70-71 6.246630148964982E-4 1102 72-73 0.10234911724297646 1102 74-75 0.5152502274588997 1102 76-77 0.49480492949639654 1102 78-79 0.21978189760357836 1102 80-81 -0.18017519707442275 1102 82-83 -0.596609737209949 1102 84-85 -0.30931891658589095 1102 86-87 -0.14245316119277973 1102 88-89 0.08810049423694011 1102 90-91 -0.4911555329850117 1102 92-93 -0.06357773647808429 1102 94-95 -0.41739183289817205 1102 96-97 -0.15431241773949367 1102 98-99 0.21389400569114514 1102 100-101 0.49807641445358897 1103 1 0.43868791965672926 1103 2 0.35996932040382745 1103 3 0.1087960572257316 1103 4 0.3714041695375556 1103 5 0.3982541477297019 1103 6 0.330942698429574 1103 7 0.19109985048760336 1103 8 0.34325207081678144 1103 9 0.505137146997491 1103 10-11 0.19848115035707536 1103 12-13 0.21765446024917168 1103 14-15 0.29657118041057373 1103 16-17 0.6079075811674102 1103 18-19 0.4579585866896281 1103 20-21 0.2900924626074044 1103 22-23 0.5176113184031266 1103 24-25 0.6010892682247473 1103 26-27 0.6389749069994153 1103 28-29 0.30924621968311783 1103 30-31 0.7374775725032592 1103 32-33 1.023172933964453 1103 34-35 1.0732367211606828 1103 36-37 1.2606381117203043 1103 38-39 1.2441736067856866 1103 40-41 1.5322615163298465 1103 42-43 1.069081297252879 1103 44-45 1.1787369936703556 1103 46-47 1.3595747896671284 1103 48-49 1.7291505470196071 1103 50-51 1.7958462346182174 1103 52-53 1.5553124151721107 1103 54-55 1.5427505310354377 1103 56-57 1.7869048503752012 1103 58-59 1.5945970054600878 1103 60-61 1.934838767628321 1103 62-63 1.7917449402990187 1103 64-65 1.7810853128425315 1103 66-67 1.8357530834611993 1103 68-69 1.8011810682502691 1103 70-71 2.0066109284888256 1103 72-73 2.0344558308961815 1103 74-75 1.5227500015620876 1103 76-77 0.8802413621339689 1103 78-79 0.9697254234009982 1103 80-81 0.8546016168769661 1103 82-83 1.0387250418629677 1103 84-85 1.2332965166841916 1103 86-87 1.0828111380753178 1103 88-89 1.4301082650872168 1103 90-91 1.409811305363231 1103 92-93 1.2638371004244178 1103 94-95 1.6850975499517418 1103 96-97 1.6052543121782819 1103 98-99 1.6784507961492636 1103 100-101 1.8038277472447675 1104 1 0.3908166767927099 1104 2 0.376086889241666 1104 3 0.41005474076655446 1104 4 0.4809256863773186 1104 5 0.31353947225913004 1104 6 0.6139745438109614 1104 7 0.4448999742925466 1104 8 0.3384603611622907 1104 9 0.534781551702082 1104 10-11 0.5099078876384127 1104 12-13 0.5061658204986159 1104 14-15 0.5608603337223599 1104 16-17 0.4024830907164372 1104 18-19 0.7854111854469963 1104 20-21 0.8562822509926633 1104 22-23 0.8896566218398689 1104 24-25 1.026680207537396 1104 26-27 0.8056415736660867 1104 28-29 0.8852372323616606 1104 30-31 1.189056085468387 1104 32-33 0.8506599229824943 1104 34-35 1.3693597924402283 1104 36-37 1.1551287919596547 1104 38-39 1.2223357912550412 1104 40-41 1.755374064189617 1104 42-43 1.7279757650062777 1104 44-45 1.9108139361469156 1104 46-47 2.003085347477146 1104 48-49 2.4459008966539173 1104 50-51 2.2277993721563334 1104 52-53 1.9722370087938685 1104 54-55 1.9133858337843606 1104 56-57 2.0793252369217328 1104 58-59 2.239551954218598 1104 60-61 1.923799493950625 1104 62-63 1.636037303368468 1104 64-65 1.6701537953149632 1104 66-67 1.6879208162259545 1104 68-69 1.798017164267879 1104 70-71 1.8795389152829642 1104 72-73 2.0086631353875504 1104 74-75 1.8335118604703098 1104 76-77 1.2246483594056734 1104 78-79 1.3517324390141745 1104 80-81 1.2355769246697967 1104 82-83 1.4100481498254602 1104 84-85 1.0732900971688686 1104 86-87 1.6332272602295212 1104 88-89 1.0478605176492906 1104 90-91 1.359005426921339 1104 92-93 1.4384708441481955 1104 94-95 1.616775565404435 1104 96-97 2.1987385041217884 1104 98-99 1.4031682228406908 1104 100-101 1.814362630432516 1105 1 -0.11880809653690605 1105 2 0.09031946468222074 1105 3 0.3172244994497717 1105 4 0.43187188993895376 1105 5 0.09040281907644498 1105 6 0.28645327166700696 1105 7 0.41044607556190016 1105 8 0.3316021289588207 1105 9 0.4078461353260181 1105 10-11 0.10072715578366598 1105 12-13 0.3105202167417005 1105 14-15 -0.01041878470419988 1105 16-17 0.22827236862595157 1105 18-19 0.11273484751050233 1105 20-21 0.508232778727816 1105 22-23 0.4433833154744917 1105 24-25 0.6500470183431801 1105 26-27 0.5011244397096917 1105 28-29 0.9314198705724763 1105 30-31 0.3948379239395834 1105 32-33 0.5633232419019123 1105 34-35 0.575153255033797 1105 36-37 0.5966129599049736 1105 38-39 0.7045508840155392 1105 40-41 0.4154498534675284 1105 42-43 0.6100606675961302 1105 44-45 0.7115764785749548 1105 46-47 0.646968734281451 1105 48-49 0.3017850274020475 1105 50-51 0.8282550377535856 1105 52-53 1.0133747778923023 1105 54-55 0.9555697738968831 1105 56-57 1.1037358939171327 1105 58-59 0.6144299009336223 1105 60-61 0.9447229142323934 1105 62-63 0.5874833441923784 1105 64-65 0.8501882027172023 1105 66-67 0.36112023966948215 1105 68-69 0.8911612568888891 1105 70-71 0.6967030943874448 1105 72-73 0.32167750280281027 1105 74-75 0.6792597565041625 1105 76-77 0.5851705149829272 1105 78-79 0.9476314486389477 1105 80-81 0.6988563220752937 1105 82-83 0.404352329859865 1105 84-85 0.04419491885482785 1105 86-87 -0.48453672358970223 1105 88-89 -0.45569646379860984 1105 90-91 -0.1225005943740527 1105 92-93 0.17222032106268514 1105 94-95 0.05507472381797385 1105 96-97 -0.30950299864475284 1105 98-99 0.2552628896933413 1105 100-101 0.8902332771986856 1106 1 0.3710563236289488 1106 2 0.21940550741839004 1106 3 0.5601493382019136 1106 4 0.0955987996840264 1106 5 0.06723577945554382 1106 6 -0.07763781692059268 1106 7 0.18290011443689025 1106 8 0.46536106889124795 1106 9 0.4217551344923578 1106 10-11 0.20915811537297913 1106 12-13 0.08149340791365489 1106 14-15 0.3078647482693455 1106 16-17 0.439726451377922 1106 18-19 0.38673554954200995 1106 20-21 0.3726090676137801 1106 22-23 0.30784735829841736 1106 24-25 0.6463284452222098 1106 26-27 0.5356885220228875 1106 28-29 0.29607039051455786 1106 30-31 0.6556316583084083 1106 32-33 0.44080777527035053 1106 34-35 0.14544415933813326 1106 36-37 -0.052985565828869596 1106 38-39 0.01660388739406926 1106 40-41 0.174685955437603 1106 42-43 0.5285153207486957 1106 44-45 -0.30465799994726694 1106 46-47 -0.09189748484013194 1106 48-49 -0.2291043587591517 1106 50-51 -0.39156857683111923 1106 52-53 0.12198124535753152 1106 54-55 -0.41675921561652274 1106 56-57 -0.36770019927755015 1106 58-59 -0.29902277775534003 1106 60-61 0.18986353991797245 1106 62-63 0.22860264567103172 1106 64-65 -0.021493601503760118 1106 66-67 0.18820110492330855 1106 68-69 -0.3568423410225705 1106 70-71 -0.44766310279565147 1106 72-73 -0.6887652158141897 1106 74-75 -0.16743411388327445 1106 76-77 -0.12066731573972689 1106 78-79 -0.03874475249307707 1106 80-81 -0.30082556989960807 1106 82-83 0.044166291511398725 1106 84-85 0.6309945327374997 1106 86-87 0.41278019975723623 1106 88-89 0.4860568598817885 1106 90-91 0.2640225946040502 1106 92-93 0.32264153996488787 1106 94-95 -0.13853240180178972 1106 96-97 -0.19604812610734612 1106 98-99 -0.10362877807024518 1106 100-101 0.12849618799680584 1107 1 -0.30155855114002605 1107 2 -0.22401216300839621 1107 3 -0.10449918643004352 1107 4 -0.12748024775293487 1107 5 -0.051940818594296445 1107 6 0.005275955305528157 1107 7 -0.05439090164450988 1107 8 0.10791624914320153 1107 9 -0.41449919389121703 1107 10-11 0.2945255234413011 1107 12-13 0.2719635287897404 1107 14-15 -0.1373904935890451 1107 16-17 -0.27298571908072944 1107 18-19 -0.28335133678348257 1107 20-21 -0.07676743271549213 1107 22-23 0.24240644737283645 1107 24-25 0.32445603428600833 1107 26-27 0.3584319205740769 1107 28-29 0.33325731295417427 1107 30-31 0.4430575712521332 1107 32-33 -0.06260182258829872 1107 34-35 -0.22486366043115424 1107 36-37 -0.17012277824363053 1107 38-39 0.2734602594119977 1107 40-41 0.03752664967846897 1107 42-43 0.10139149180216833 1107 44-45 -0.12459724325022137 1107 46-47 0.08429693659041249 1107 48-49 0.11199351050772322 1107 50-51 0.1280605099064971 1107 52-53 0.3394732258983879 1107 54-55 0.22092055835156543 1107 56-57 0.10107170818532296 1107 58-59 0.3631083802333208 1107 60-61 0.23655697352109684 1107 62-63 0.2123300477911485 1107 64-65 -0.2146191724255182 1107 66-67 -0.060497724554309684 1107 68-69 -0.19357465041436583 1107 70-71 0.07566237040554569 1107 72-73 -0.2504035222743646 1107 74-75 0.1902125200682505 1107 76-77 0.4761397711253572 1107 78-79 0.6362600273170038 1107 80-81 0.14919886024081208 1107 82-83 -0.007243221372846165 1107 84-85 0.3975061211215021 1107 86-87 -0.06779252770861532 1107 88-89 0.7291261352625789 1107 90-91 0.6566574383573709 1107 92-93 -0.07564410148562573 1107 94-95 -0.08896046034915273 1107 96-97 -0.25819627897629616 1107 98-99 -0.5655177590147389 1107 100-101 -1.079223736375976 1108 1 0.20516474775863358 1108 2 0.4251810411237642 1108 3 0.29121497731963686 1108 4 0.15237973687952433 1108 5 0.03913320172500789 1108 6 0.30483627290136184 1108 7 0.3588678706597648 1108 8 0.5909479233513437 1108 9 0.506550067613091 1108 10-11 0.5686120085433259 1108 12-13 0.6569097423985539 1108 14-15 0.7706603900445188 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-2.3505391111206 2316 66-67 -2.5308943819765872 2316 68-69 -2.959475613777375 2316 70-71 -2.912167873354491 2316 72-73 -3.1536202059701814 2316 74-75 -3.406397463875699 2316 76-77 -3.383871541091839 2316 78-79 -3.182060290884717 2316 80-81 -2.4797877840446922 2316 82-83 -2.460698921270673 2316 84-85 -3.2425099349287088 2316 86-87 -3.4961059126159277 2316 88-89 -4.266896343207716 2316 90-91 -3.917665653162114 2316 92-93 -4.121531563170052 2316 94-95 -4.7751559062694575 2316 96-97 -4.63264891362817 2316 98-99 -5.054844134726313 2316 100-101 -5.91621833570454 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1936.0 3 359.0 4 303.0 5 296.0 6 313.0 7 364.0 8 506.0 9 578.0 10 707.0 11 893.0 12 1257.0 13 1766.0 14 1811.0 15 1916.0 16 2290.0 17 2683.0 18 2472.0 19 1917.0 20 1990.0 21 2073.0 22 2308.0 23 2574.0 24 2927.0 25 3234.0 26 3565.0 27 4052.0 28 4744.0 29 5420.0 30 6448.0 31 7510.0 32 9170.0 33 11134.0 34 14929.0 35 22860.0 36 27331.0 37 35304.0 38 24700.0 39 2674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.64283769879902 20.365970129971995 16.050180170619964 14.941012000609028 2 14.135380590966031 27.865485225849234 32.589252818510055 25.409881364674675 3 28.716701669061568 25.694981761910025 32.48360414133599 13.104712427692423 4 11.642100414555506 18.297098111469367 42.982035928143716 27.078765545831413 5 9.122734286832953 42.5905709482024 35.23941131762592 13.047283447338723 6 23.312502014819863 38.6882256229823 15.797569321316566 22.20170304088127 7 20.134855679551954 37.18295328411347 24.392850069776763 18.28934096655782 8 22.055497985031664 25.004490500863557 21.22832469775475 31.711686816350028 9 18.222641857037583 25.61394620486367 29.322033898305083 26.84137803979366 10-11 24.458844552522013 24.471049334954497 26.588003389705612 24.482102722817878 12-13 31.961229527827417 24.714909452662994 25.7113446694055 17.612516350104087 14-15 23.69797364051521 28.522869374774025 32.55209832645137 15.22705865825939 16-17 16.430018882697002 31.099341408372865 32.475936075162345 19.994703633767788 18-19 16.110348575850253 35.818149158038246 30.086314897380156 17.985187368731346 20-21 21.034983072706755 27.90769443356901 26.56387462287833 24.493447870845905 22-23 17.502913658956796 28.06740341162976 30.971849217573165 23.45783371184028 24-25 24.838519446773613 28.52930607130023 27.555815603490373 19.07635887843578 26-27 21.595652534485776 23.886180809597963 37.10319471448449 17.41497194143177 28-29 16.19764541117773 35.06176707030827 30.507151067304573 18.23343645120943 30-31 18.722850496349356 28.151185019692747 29.143192758596864 23.982771725361037 32-33 19.58123937667267 29.237009023782434 32.20808593552072 18.973665664024175 34-35 23.678723576203286 26.6894489534387 29.714721128646804 19.91710634171121 36-37 20.22810873337189 25.639560439560437 35.85378831694621 18.278542510121458 38-39 19.446442656205544 29.654824659809265 32.926854655345096 17.97187802864009 40-41 18.383730017433443 31.274537458786316 31.34205159744926 18.999680926330974 42-43 17.080583013779737 31.536817662486644 26.603819841156035 24.778779482577583 44-45 20.36203805447921 26.066739922503036 28.88415938927766 24.687062633740094 46-47 24.15264724727457 30.45728019023078 29.594024754983035 15.796047807511618 48-49 21.056576883389706 28.183172692063874 31.044638684391963 19.715611740154458 50-51 14.071757240383748 30.428928684620242 36.27745145894976 19.221862616046252 52-53 18.982158390553572 33.20269845088931 27.080287242508934 20.734855916048193 54-55 16.245139112189637 27.191361094853534 30.81519488661206 25.748304906344778 56-57 22.008616682507856 25.973076914182375 28.775001907880586 23.243304495429182 58-59 26.31320921391291 25.650216334091063 28.419431449496223 19.61714300249981 60-61 20.06753664591105 28.385961992723978 34.108779763968435 17.437721597396532 62-63 17.280429668796966 28.68691494750574 33.27435102108791 20.75830436260938 64-65 16.84482822449282 33.7963220050348 29.53039389900785 19.828455871464534 66-67 19.729766003924617 27.71344736939539 26.729053278536785 25.827733348143212 68-69 16.637046910289165 27.898570846754783 30.890397645229367 24.573984597726685 70-71 23.386068553472217 27.892743180172115 27.29759185855206 21.42359640780361 72-73 20.227771247697763 23.505048728793952 35.31333586309662 20.953844160411673 74-75 16.30972984479264 33.550750122629545 29.097677905394775 21.04184212718304 76-77 18.71548638888439 26.466662194018838 29.05701931952519 25.760832097571583 78-79 19.271671755018964 26.85470441298918 32.02493292626515 21.84869090572671 80-81 22.369957837915347 26.056538361021236 29.420253990133656 22.15324981092976 82-83 19.04487637658129 24.957149895563713 34.23705996238906 21.76091376546594 84-85 18.498160871677424 27.44233927869156 31.880768963842044 22.178730885788976 86-87 18.639951145942245 28.60252412631735 30.38528086456878 22.37224386317163 88-89 18.25685632655894 27.798456965056157 27.58076642800134 26.363920280383564 90-91 20.16279048244843 23.355899419729205 29.96168546916791 26.51962462865446 92-93 22.648359352765187 27.36656508791537 30.37713714519745 19.607938414121993 94-95 20.1393853992406 25.243473250914544 31.193267666706003 23.423873683138854 96-97 15.513399829248486 26.146036505655857 34.50036672242698 23.840196942668676 98-99 19.45148235816772 28.197083180041787 28.202173616269032 24.149260845521457 100-101 18.028755508773553 23.70351520479336 31.09550854221369 27.1722207442194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4796.0 1 2798.0 2 710.5 3 541.0 4 409.5 5 421.0 6 660.0 7 1127.5 8 1441.5 9 1340.0 10 957.0 11 621.0 12 539.0 13 495.0 14 451.5 15 456.0 16 534.5 17 555.5 18 461.5 19 412.0 20 423.5 21 467.0 22 526.0 23 574.5 24 641.5 25 772.5 26 1008.5 27 1284.0 28 1406.0 29 1552.5 30 1855.0 31 2118.0 32 2516.0 33 3208.5 34 3730.0 35 4127.5 36 4800.5 37 5143.5 38 4834.5 39 4339.0 40 3830.0 41 3443.5 42 3129.0 43 3026.0 44 3063.5 45 3440.5 46 6569.0 47 17668.5 48 37814.0 49 37501.0 50 17504.5 51 6781.0 52 3103.5 53 1755.5 54 1072.0 55 700.0 56 522.5 57 451.5 58 409.0 59 394.5 60 400.5 61 392.5 62 340.5 63 256.5 64 185.0 65 144.0 66 119.5 67 108.5 68 87.5 69 77.0 70 74.0 71 56.0 72 37.5 73 35.5 74 38.0 75 29.5 76 24.5 77 23.0 78 16.0 79 11.0 80 7.5 81 4.0 82 2.5 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26459408965828246 2 0.08190912688552049 3 0.08559043595902703 4 0.0984750177162999 5 0.1007758358872415 6 0.0796083087145789 7 0.08881158139834526 8 0.08697092686159198 9 0.08927174503253357 10-11 0.08559043595902703 12-13 0.08743109049578031 14-15 0.09226280865475762 16-17 0.08467010869065039 18-19 0.0929530541060401 20-21 0.09617419954535833 22-23 0.10721812676587794 24-25 0.11964254488896252 26-27 0.12332385396246906 28-29 0.11435066309579686 30-31 0.10537747222912468 32-33 0.10169616315561814 34-35 0.3550162437762868 36-37 0.5471345610499094 38-39 0.5284979338652825 40-41 0.48915394314218136 42-43 0.5186044157302336 44-45 0.5413825156225553 46-47 0.5317190793046007 48-49 0.5526565246601692 50-51 0.5441434974276853 52-53 0.5475947246840976 54-55 0.540922351988367 56-57 0.5087108975951848 58-59 0.5050295885216783 60-61 0.4541815069438692 62-63 0.5280377702310942 64-65 0.5604793064413706 66-67 0.5715232336618902 68-69 0.5715232336618902 70-71 0.5680720064054778 72-73 0.5613996337097472 74-75 0.5595589791729939 76-77 0.5455239883302502 78-79 0.5218255611695519 80-81 0.5174540066447628 82-83 0.5307987520362241 84-85 0.5425329247080262 86-87 0.5337898156584481 88-89 0.543683333793497 90-91 0.5558776700994874 92-93 0.5639305336977829 94-95 0.562550042795218 96-97 0.5570280791849582 98-99 0.562550042795218 100-101 0.543683333793497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 217314.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.119476885980653 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97605647238386 18.56622214859604 2 9.44958504244968 4.558380960269472 3 3.9721453782314224 2.8741820591402303 4 2.23218544309835 2.1535658080013254 5 1.3545740723075457 1.6335809013685267 6 0.9596489554516837 1.3887738479803418 7 0.7650481732328531 1.2916793211666067 8 0.5609081369836879 1.0823048676109224 9 0.39110941524372794 0.8490019050774456 >10 2.9762472574644665 13.046099192872987 >50 0.17552227415816082 2.963913967806952 >100 0.11637889917008491 5.9927110080344566 >500 0.019078508060669658 3.3444692932806905 >1k 0.04578841934560717 27.734522396164074 >5k 0.003815701612133931 6.46529906034586 >10k+ 0.0019078508060669656 6.055293262284069 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13159 6.055293262284069 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8547 3.9330185814075485 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5503 2.5322804789383104 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4077 1.87608713658577 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3877 1.7840544097481064 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3869 1.7803731006746 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3761 1.7306754281822614 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3720 1.7118087191805405 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3612 1.6621110466882023 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3467 1.5953873197308965 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3450 1.5875645379496948 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3036 1.3970567933957316 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2996 1.3786502480281988 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2567 1.1812400489614108 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2521 1.1600725217887482 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2353 1.0827650312451107 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2110 0.9709452681373496 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2100 0.9663436317954666 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1988 0.9148053047663749 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1664 0.7657122872893601 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1608 0.7399431237748143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1395 0.6419282696927028 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1386 0.6377867969850078 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1367 0.6290436879354299 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1283 0.5903899426636111 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1053 0.48455230680029815 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1011 0.46522543416438883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.4201293980139338 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 893 0.4109261253301674 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 838 0.38561712544980997 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 826 0.3800951618395501 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 778 0.35800730739851094 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 689 0.31705274395575067 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 668 0.307389307637796 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 603 0.2774786714155554 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 535 0.24618754429074982 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 525 0.24158590794886664 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 485 0.22317936258133392 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 433 0.1992508536035414 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 395 0.18176463550438535 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 0.1744020173573723 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 367 0.16888005374711249 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 363 0.1670393992103592 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 358 0.1647385810394176 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 342 0.15737596289240452 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 337 0.15507514472146294 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 336 0.15461498108727462 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 335 0.15415481745308632 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 316 0.14541170840350828 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 308 0.14173039933000176 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.13574827208555362 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 289 0.13298729028042372 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 283 0.13022630847529382 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 274 0.12608483576759894 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 273 0.12562467213341064 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 272 0.1251645084992223 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 269 0.12378401759665739 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 246 0.11320025401032607 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 240 0.11043927220519617 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 240 0.11043927220519617 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 233 0.10721812676587794 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 226 0.10399698132655971 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 222 0.10215632678980645 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.3008181709415867E-4 0.0 16-17 0.0 0.0 0.0 0.0011504090854707934 0.0 18-19 0.0 0.0 0.0 0.0025308999880357453 0.0 20-21 0.0 0.0 0.0 0.005982127244448125 0.0 22-23 0.0 0.0 0.0 0.011043927220519617 0.0 24-25 0.0 0.0 0.0 0.018406545367532695 0.0 26-27 0.0 0.0 0.0 0.035202518015406276 0.0 28-29 0.0 0.0 0.0 0.10031567225305318 0.0 30-31 0.0 0.0 0.0 0.23238263526510028 0.0 32-33 0.0 0.0 0.0 0.45050019787036266 0.0 34-35 0.0 0.0 0.0 0.7431642692141325 0.0 36-37 0.0 0.0 0.0 1.0809243767083574 0.0 38-39 0.0 0.0 0.0 1.5323449018470967 0.0 40-41 0.0 0.0 0.0 1.9701905997772808 0.0 42-43 0.0 0.0 0.0 2.388019179620273 0.0 44-45 0.0 0.0 0.0 2.887756886348786 0.0 46-47 0.0 0.0 0.0 3.4578996291081108 0.0 48-49 0.0 0.0 0.0 3.9017274542827427 0.0 50-51 0.0 0.0 0.0 4.377306570216369 0.0 52-53 0.0 0.0 0.0 4.806869322731163 0.0 54-55 0.0 0.0 0.0 5.319951774851138 0.0 56-57 0.0 0.0 0.0 6.0433290077951725 0.0 58-59 0.0 0.0 0.0 6.5927643870160235 0.0 60-61 0.0 0.0 0.0 7.229170693098466 0.0 62-63 0.0 0.0 0.0 7.824622435738148 0.0 64-65 0.0 0.0 0.0 8.427666878341938 0.0 66-67 0.0 0.0 0.0 9.147132720395373 0.0 68-69 0.0 0.0 0.0 9.999125689095042 0.0 70-71 0.0 0.0 0.0 10.845136530550263 0.0 72-73 0.0 0.0 0.0 11.588300799764395 0.0 74-75 0.0 0.0 0.0 12.525424040788906 0.0 76-77 0.0 0.0 0.0 13.272269619076543 0.0 78-79 0.0 0.0 0.0 14.497685376920032 0.0 80-81 0.0 0.0 0.0 15.647634298756637 0.0 82-83 0.0 0.0 0.0 16.558758294449504 0.0 84-85 0.0 0.0 0.0 17.674194943721986 0.0 86-87 0.0 0.0 0.0 18.744075393209826 0.0 88-89 0.0 0.0 0.0 19.914731678584907 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCCT 30 1.32458E-4 63.082817 5 GATCCTC 30 1.32458E-4 63.082817 6 GTACCCA 45 1.5309412E-5 52.666027 1 CGTATCC 20 5.3426943E-4 47.57502 92-93 TAAGATC 40 5.4908276E-4 47.30122 3 TTTAAGA 45 9.708516E-4 42.13282 1 CCTCATC 45 9.797044E-4 42.055214 9 AAGATCC 45 9.808155E-4 42.04553 4 GTAAGCG 60 8.54925E-11 39.64585 94-95 TCCTCAT 50 0.0016446421 37.84969 8 GGGCCAA 50 0.0016446421 37.84969 8 GGGGCCA 50 0.0016446421 37.84969 7 ACCCATG 50 0.001646504 37.840977 3 TAGGTGG 55 0.0026255758 34.40881 5 TACCCAT 55 0.0026285427 34.400887 2 ATAGGTG 60 0.0040257582 31.534145 4 TATAGGT 60 0.0040257582 31.534145 3 CATGGGG 185 9.094947E-12 30.681873 4 GGTGGAT 65 0.0059478534 29.115147 7 CCCACGA 35 0.008321573 27.185724 92-93 >>END_MODULE