FastQCFastQC Report
Fri 27 May 2016
SRR938959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959347
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43790.14797183297531516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35670.1205333473904885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93850.027.4115051
TACATGG82300.025.2109472
GTACATG86150.025.0131511
GGTATCA71400.024.8622971
ATCAACG107500.023.5313953
TCAACGC108000.023.378594
ACATGGG82900.023.3713423
TATCAAC108750.023.217362
CAACGCA108550.023.1292155
AACGCAG112100.022.5973456
ACGCAGA121650.020.4341437
CGCAGAG122600.019.8123578
CATGGGG48200.019.361284
GAGTACT53300.019.18891712-13
GTGTAGC18400.018.8309781
GTATAGG9000.018.458371
GTACTTT57900.017.41936514-15
CATGGGA39650.017.0847154
GTAGCCC16650.016.786213
TAGCCCT22050.016.7571774
Produced by FastQC (version 0.11.5)