##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938959_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2959347 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.572420537368547 33.0 31.0 34.0 30.0 34.0 2 31.66347508419932 33.0 31.0 34.0 30.0 34.0 3 31.7155825254693 33.0 31.0 34.0 28.0 34.0 4 35.25780315725057 37.0 35.0 37.0 33.0 37.0 5 35.294339257951165 37.0 35.0 37.0 33.0 37.0 6 35.36195856721094 37.0 35.0 37.0 33.0 37.0 7 35.26895122471275 37.0 35.0 37.0 32.0 37.0 8 35.247411337703895 37.0 35.0 37.0 32.0 37.0 9 36.827900546978775 39.0 37.0 39.0 33.0 39.0 10-11 36.69776525023933 39.0 37.0 39.0 32.0 39.0 12-13 36.578795761362215 39.0 37.0 39.0 32.0 39.0 14-15 37.763731661072526 40.0 38.0 41.0 32.0 41.0 16-17 37.66557301323569 40.0 37.0 41.0 32.0 41.0 18-19 37.550363982324484 40.0 37.0 41.0 32.0 41.0 20-21 37.651314293322145 40.0 37.0 41.0 32.0 41.0 22-23 37.58493258816895 40.0 37.0 41.0 32.0 41.0 24-25 37.50281345850959 40.0 37.0 41.0 32.0 41.0 26-27 37.38837402305306 40.0 37.0 41.0 31.0 41.0 28-29 37.25730794665175 40.0 37.0 41.0 31.0 41.0 30-31 37.07535226521256 40.0 37.0 41.0 30.5 41.0 32-33 36.89763231550744 40.0 36.0 41.0 30.0 41.0 34-35 36.58991882330798 39.0 36.0 41.0 30.0 41.0 36-37 36.33649298308039 39.0 35.5 41.0 30.0 41.0 38-39 36.10101856929924 39.0 35.0 41.0 28.5 41.0 40-41 35.95744415913376 39.0 35.0 40.0 28.5 41.0 42-43 35.76896136208427 39.0 35.0 40.0 27.5 41.0 44-45 35.77859744058402 39.0 35.0 41.0 27.0 41.0 46-47 35.641798173718726 39.0 35.0 41.0 27.0 41.0 48-49 35.454909985209575 39.0 35.0 41.0 26.0 41.0 50-51 34.377198077819195 38.0 34.0 40.0 25.0 40.5 52-53 34.492501555241745 38.0 33.5 39.5 25.0 40.5 54-55 35.00515958419206 38.5 34.0 40.0 26.0 41.0 56-57 34.91646755179437 38.0 34.0 40.0 25.5 41.0 58-59 34.70356805065442 38.0 34.0 40.0 25.0 41.0 60-61 34.390883022504624 38.0 34.0 40.0 24.5 41.0 62-63 33.9986419301285 37.5 33.5 40.0 23.0 41.0 64-65 33.60304705734069 37.0 33.0 40.0 22.0 41.0 66-67 33.19293208941026 36.0 32.5 39.0 21.5 41.0 68-69 32.73447993763489 36.0 32.0 39.0 20.5 40.5 70-71 32.28699963201342 35.0 31.5 38.5 19.5 40.0 72-73 31.84322385985827 35.0 31.0 37.5 18.0 40.0 74-75 31.357731452242675 35.0 31.0 37.0 15.0 39.0 76-77 30.911778848509485 35.0 30.5 36.5 11.5 39.0 78-79 30.504566547958046 34.5 30.0 36.0 7.5 39.0 80-81 30.052935495567098 34.0 30.0 36.0 6.0 37.5 82-83 29.56883765236047 34.0 29.0 35.0 2.0 37.0 84-85 29.136994073354696 34.0 29.0 35.0 2.0 36.5 86-87 28.66446550539697 34.0 29.0 35.0 2.0 36.0 88-89 28.16625610312005 34.0 27.5 35.0 2.0 36.0 90-91 27.68170545731879 33.5 26.5 35.0 2.0 35.0 92-93 27.153087995426016 33.0 25.5 35.0 2.0 35.0 94-95 26.586293361339514 33.0 25.0 35.0 2.0 35.0 96-97 25.894378895073814 32.5 23.5 35.0 2.0 35.0 98-99 24.97045344800728 32.0 17.5 34.5 2.0 35.0 100-101 21.80929779441208 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36218732420144306 1101 2 0.4796811968217902 1101 3 0.48348303445515484 1101 4 0.4486458053209148 1101 5 0.3191309820087014 1101 6 0.44198636788250667 1101 7 0.5111206390634067 1101 8 0.340629867489902 1101 9 0.56541189474531 1101 10-11 0.46894628796804483 1101 12-13 0.5133383987542572 1101 14-15 0.6526095009316961 1101 16-17 0.5998056384546189 1101 18-19 0.7170074473876227 1101 20-21 0.6023815146493448 1101 22-23 0.6094007998973652 1101 24-25 0.6550929959851857 1101 26-27 0.6298748042629612 1101 28-29 0.673414585601158 1101 30-31 0.6514066824510749 1101 32-33 0.7011656504399397 1101 34-35 0.5826729217872 1101 36-37 0.6570754772990242 1101 38-39 0.6565281937157224 1101 40-41 0.5977978721126433 1101 42-43 0.5527473704178405 1101 44-45 0.732799405201952 1101 46-47 0.6742612662849439 1101 48-49 0.5988062036046813 1101 50-51 0.6239018317459895 1101 52-53 0.6581821354082251 1101 54-55 0.7112124357246046 1101 56-57 0.6939765585310198 1101 58-59 0.6278729343343556 1101 60-61 0.5990656681880822 1101 62-63 0.5529107211107274 1101 64-65 0.6033698534826684 1101 66-67 0.5419606921358948 1101 68-69 0.5071118550469507 1101 70-71 0.3987536422977911 1101 72-73 0.1988808962075268 1101 74-75 0.0783523419950427 1101 76-77 0.23459299173045167 1101 78-79 0.30678062627575997 1101 80-81 0.3230446922844301 1101 82-83 0.3712394851051606 1101 84-85 0.10340760166674912 1101 86-87 0.04842399313639234 1101 88-89 0.1240364735457753 1101 90-91 0.09026542762581258 1101 92-93 -0.053386830292264875 1101 94-95 -0.1585904133142364 1101 96-97 -0.28501137480786554 1101 98-99 -0.3621052608456239 1101 100-101 0.22692544740637643 1102 1 0.3241608349666194 1102 2 0.3877786169460755 1102 3 0.3938192761641872 1102 4 0.34792184992996056 1102 5 0.4673095855698932 1102 6 0.5020767576885419 1102 7 0.5227541418776269 1102 8 0.5755240988627008 1102 9 0.6150028390258555 1102 10-11 0.6155859632342953 1102 12-13 0.6240136022359337 1102 14-15 0.6484666348215953 1102 16-17 0.7862314748741781 1102 18-19 0.7420299402213004 1102 20-21 0.7143675795542492 1102 22-23 0.8158431659342327 1102 24-25 0.7215849508739751 1102 26-27 0.779120509700995 1102 28-29 0.6611178057380016 1102 30-31 0.7482744245890416 1102 32-33 0.7600916437862466 1102 34-35 0.5983551346635636 1102 36-37 0.6335762200856223 1102 38-39 0.738901135359896 1102 40-41 0.6556075932015091 1102 42-43 0.49667953621617755 1102 44-45 0.6781575120376786 1102 46-47 0.8380279266745418 1102 48-49 0.8088840392709926 1102 50-51 0.7344284779075636 1102 52-53 0.6143671415806793 1102 54-55 0.7044143690621212 1102 56-57 0.7093700261936533 1102 58-59 0.6403799960379501 1102 60-61 0.7525463034275575 1102 62-63 0.7642230471835774 1102 64-65 0.6514218694682725 1102 66-67 0.7255346464806749 1102 68-69 0.8887169018111223 1102 70-71 0.8195778215709097 1102 72-73 0.6271245720596426 1102 74-75 0.5415697587715549 1102 76-77 0.4281841033995235 1102 78-79 0.4450853989412167 1102 80-81 0.480939153816454 1102 82-83 0.4504927230270326 1102 84-85 0.28344024243004284 1102 86-87 0.3112449083331761 1102 88-89 0.17682244638698563 1102 90-91 0.3669890063925756 1102 92-93 0.2354546318465438 1102 94-95 0.1451172426186389 1102 96-97 0.06395709337785149 1102 98-99 -7.521338186542437E-4 1102 100-101 -0.02232758127982848 1103 1 0.37763551984971855 1103 2 0.3205109573345375 1103 3 0.4437058382273875 1103 4 0.409994176465311 1103 5 0.2909216817453597 1103 6 0.3213122647996727 1103 7 0.3629854142489535 1103 8 0.27061578243099405 1103 9 0.516223074528817 1103 10-11 0.48063152505277884 1103 12-13 0.3982758408828033 1103 14-15 0.5298329501832058 1103 16-17 0.5975497605718729 1103 18-19 0.6528892896225855 1103 20-21 0.5853256012961765 1103 22-23 0.5367091100821213 1103 24-25 0.6505327777183254 1103 26-27 0.6174799524241692 1103 28-29 0.6219313237242403 1103 30-31 0.6323520518075085 1103 32-33 0.7152919540296736 1103 34-35 0.5605635037702825 1103 36-37 0.6103013269910278 1103 38-39 0.8276624843381271 1103 40-41 0.8504877471618855 1103 42-43 0.7412751621156914 1103 44-45 0.8447337601660578 1103 46-47 0.8727805822006545 1103 48-49 0.9511308569946877 1103 50-51 0.7312219379098011 1103 52-53 0.7949033069221727 1103 54-55 0.7972372938944545 1103 56-57 0.816287044372757 1103 58-59 0.8248348510572185 1103 60-61 1.055038619925618 1103 62-63 0.9924728263217872 1103 64-65 0.8680142933902033 1103 66-67 0.8988717541658318 1103 68-69 0.8018638817075043 1103 70-71 0.8371955419453556 1103 72-73 0.7811042320361707 1103 74-75 0.6635628239185252 1103 76-77 0.7736770710769711 1103 78-79 0.6848027787944737 1103 80-81 0.6945008976499523 1103 82-83 0.8672269962154466 1103 84-85 0.8523188451732899 1103 86-87 1.0065373376470355 1103 88-89 0.8145489593421367 1103 90-91 0.6894669305820216 1103 92-93 0.6267421005837122 1103 94-95 0.5431301085959674 1103 96-97 0.5556703172964568 1103 98-99 0.6682747470321111 1103 100-101 0.7574197030532908 1104 1 0.30830312114430924 1104 2 0.28435049707930915 1104 3 0.1860886683063434 1104 4 0.21324083749208 1104 5 0.24657328720437732 1104 6 0.28648471141293186 1104 7 0.2684402562928838 1104 8 0.2923486049324353 1104 9 0.3835207512077403 1104 10-11 0.34841939120219223 1104 12-13 0.33426369997893346 1104 14-15 0.37133808239887856 1104 16-17 0.30701868249136766 1104 18-19 0.4262153300259044 1104 20-21 0.34553723515350754 1104 22-23 0.4166987452980919 1104 24-25 0.34312025509302657 1104 26-27 0.3924313794133738 1104 28-29 0.44326931222357757 1104 30-31 0.3671312942986589 1104 32-33 0.42117936104855147 1104 34-35 0.3663896507217359 1104 36-37 0.49539635362398116 1104 38-39 0.3914764389158094 1104 40-41 0.2926669035158511 1104 42-43 0.3708766966266026 1104 44-45 0.40841087018322497 1104 46-47 0.4145863231091056 1104 48-49 0.37368378009661996 1104 50-51 0.27867227865275623 1104 52-53 0.33668052152670924 1104 54-55 0.5000215292376851 1104 56-57 0.42304050725499565 1104 58-59 0.41182128737840884 1104 60-61 0.44990482475210314 1104 62-63 0.4070407671951273 1104 64-65 0.49194059122469724 1104 66-67 0.4963390541028332 1104 68-69 0.3980740498983266 1104 70-71 0.3518185746686342 1104 72-73 0.3827816467877909 1104 74-75 0.20445456571013665 1104 76-77 0.15989497200668268 1104 78-79 -0.005333410818437301 1104 80-81 0.07515256097856593 1104 82-83 0.2368546387622068 1104 84-85 0.16970869789722443 1104 86-87 0.07388746459929862 1104 88-89 0.009296470234897214 1104 90-91 0.018369427675267502 1104 92-93 -0.04078076369140504 1104 94-95 0.062191943138230954 1104 96-97 0.022110448345891598 1104 98-99 0.05950708199927135 1104 100-101 0.3582089897990173 1105 1 0.3008064847389349 1105 2 0.23630204539432498 1105 3 0.2806823699588179 1105 4 0.2960949968057207 1105 5 0.14789590303277578 1105 6 0.26975789367863 1105 7 0.3120597760219965 1105 8 0.2355717910460342 1105 9 0.27887129482301276 1105 10-11 0.3449617884168461 1105 12-13 0.2911497809334236 1105 14-15 0.3853634095366516 1105 16-17 0.4549755807131035 1105 18-19 0.44651691244127534 1105 20-21 0.5385262301618567 1105 22-23 0.3126596453349819 1105 24-25 0.4203044022791431 1105 26-27 0.27079391255174556 1105 28-29 0.43010710867767443 1105 30-31 0.4344281981993632 1105 32-33 0.5577225278430475 1105 34-35 0.6251718835116762 1105 36-37 0.5967178416198564 1105 38-39 0.624240504314173 1105 40-41 0.6568881417963439 1105 42-43 0.5798242563950922 1105 44-45 0.6700276114637589 1105 46-47 0.6998149015889936 1105 48-49 0.8014742367759098 1105 50-51 0.7183242826121088 1105 52-53 0.7188817321554026 1105 54-55 0.70759923689819 1105 56-57 0.7519009452786065 1105 58-59 0.6345895721972497 1105 60-61 0.8343087586319271 1105 62-63 0.7321224355380664 1105 64-65 0.8326649648795907 1105 66-67 0.7621996964631563 1105 68-69 0.7947828355809605 1105 70-71 0.7267020098607233 1105 72-73 0.6906312947713644 1105 74-75 0.70490875049952 1105 76-77 0.704451853244592 1105 78-79 0.7007013556140791 1105 80-81 0.6345571582892795 1105 82-83 0.6921007848921477 1105 84-85 0.7906311189423114 1105 86-87 0.6624042056144539 1105 88-89 0.6678215572106865 1105 90-91 0.7200391505469135 1105 92-93 0.65813266919195 1105 94-95 0.5792885572523261 1105 96-97 0.6814550051607853 1105 98-99 0.6590645072505161 1105 100-101 0.9530173113453948 1106 1 0.34170118991646703 1106 2 0.39460651917299927 1106 3 0.36626026042227267 1106 4 0.31028100569361783 1106 5 0.3783594591028887 1106 6 0.36906400044465215 1106 7 0.37216786664015444 1106 8 0.3889788194640573 1106 9 0.376452906273137 1106 10-11 0.42695759725115323 1106 12-13 0.4504442848945587 1106 14-15 0.6457670051422539 1106 16-17 0.5737837527278415 1106 18-19 0.5974965784625041 1106 20-21 0.54938433215532 1106 22-23 0.6254846629034958 1106 24-25 0.47850839425071 1106 26-27 0.5869783086750431 1106 28-29 0.5678516144082977 1106 30-31 0.529866829725627 1106 32-33 0.5718492587843613 1106 34-35 0.684504431528083 1106 36-37 0.670207960276862 1106 38-39 0.8503169867684051 1106 40-41 0.8011086052007457 1106 42-43 0.6631685437199693 1106 44-45 0.774305861386658 1106 46-47 0.6012494531747166 1106 48-49 0.7671205812604072 1106 50-51 0.61762192110006 1106 52-53 0.6141847448401236 1106 54-55 0.6572134878726743 1106 56-57 0.5957992349392214 1106 58-59 0.6471066983417302 1106 60-61 0.8406276107884381 1106 62-63 0.7574825269449121 1106 64-65 0.5795558429009944 1106 66-67 0.7118614709086799 1106 68-69 0.7361804553628559 1106 70-71 0.7163411348221729 1106 72-73 0.6738842005282777 1106 74-75 0.5807236010786525 1106 76-77 0.5787599822859804 1106 78-79 0.8471513776063233 1106 80-81 0.8739092465413769 1106 82-83 0.9150649855625339 1106 84-85 0.9547982953908978 1106 86-87 0.9245465788557077 1106 88-89 0.9587142401128546 1106 90-91 1.0305051839043458 1106 92-93 1.0018888940082995 1106 94-95 0.9336253267685493 1106 96-97 0.9183951508657273 1106 98-99 0.9780828695108106 1106 100-101 1.097255722443645 1107 1 0.2620583032979873 1107 2 0.2653962405038399 1107 3 0.2255639073973832 1107 4 0.3268960126990521 1107 5 0.37576947630758184 1107 6 0.333897908977022 1107 7 0.2761690445488014 1107 8 0.3548910125277729 1107 9 0.4370424368530976 1107 10-11 0.38522168612758634 1107 12-13 0.46425248015000875 1107 14-15 0.49928219806385954 1107 16-17 0.4195740478740504 1107 18-19 0.511347480231656 1107 20-21 0.4483037035271522 1107 22-23 0.5226665946053046 1107 24-25 0.4533795543881709 1107 26-27 0.4814429194060281 1107 28-29 0.43601948787785716 1107 30-31 0.4286034347995198 1107 32-33 0.48226007640044344 1107 34-35 0.44338172147868704 1107 36-37 0.5060192399730354 1107 38-39 0.7675404578190594 1107 40-41 0.45192278815847686 1107 42-43 0.7038549831687746 1107 44-45 0.5183336643282033 1107 46-47 0.7044651633115109 1107 48-49 0.6453498798333825 1107 50-51 0.5430358129014294 1107 52-53 0.5861831655048988 1107 54-55 0.6347846324316606 1107 56-57 0.6071243968161966 1107 58-59 0.6166379571168292 1107 60-61 0.6655329800886776 1107 62-63 0.6081839490791481 1107 64-65 0.6868031553695886 1107 66-67 0.5420303142482936 1107 68-69 0.6713139248165874 1107 70-71 0.5376253629086492 1107 72-73 0.7129010814437606 1107 74-75 0.7431179885295265 1107 76-77 0.6857179453049227 1107 78-79 0.7373381337523739 1107 80-81 0.7284629231839652 1107 82-83 0.835821694196671 1107 84-85 0.8435484376238556 1107 86-87 0.9007463046940494 1107 88-89 0.8265928862211425 1107 90-91 0.8514091067015173 1107 92-93 0.8570047876195126 1107 94-95 0.7912800481457829 1107 96-97 0.8032804634876598 1107 98-99 0.746387213366372 1107 100-101 0.8573187346959443 1108 1 0.33241326730155407 1108 2 0.2593271946799973 1108 3 0.26735722271117623 1108 4 0.17366189512841856 1108 5 0.16604965198828836 1108 6 0.2768695980828113 1108 7 0.3054657311445794 1108 8 0.24503741967627235 1108 9 0.3028838694658518 1108 10-11 0.3839396146544374 1108 12-13 0.40673102698474395 1108 14-15 0.5312223880761948 1108 16-17 0.5268306462357586 1108 18-19 0.4057209090173828 1108 20-21 0.3878893459015629 1108 22-23 0.4235661609157191 1108 24-25 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2316 76-77 -2.870886611007691 2316 78-79 -2.791946292775986 2316 80-81 -2.695014263982209 2316 82-83 -2.65725415857019 2316 84-85 -2.7221275401098275 2316 86-87 -2.851243260363727 2316 88-89 -2.9939991339098633 2316 90-91 -3.2387708424171997 2316 92-93 -3.199107278610189 2316 94-95 -3.1030892518406077 2316 96-97 -3.3303854305430356 2316 98-99 -3.466699217239384 2316 100-101 -3.0359787918556336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9845.0 3 2963.0 4 3131.0 5 3406.0 6 4038.0 7 4856.0 8 5968.0 9 7400.0 10 9066.0 11 11170.0 12 13690.0 13 18080.0 14 15690.0 15 15421.0 16 17860.0 17 21447.0 18 19840.0 19 17410.0 20 18971.0 21 20774.0 22 23208.0 23 26386.0 24 30176.0 25 34818.0 26 40555.0 27 46653.0 28 55027.0 29 64826.0 30 76679.0 31 92937.0 32 114357.0 33 144301.0 34 192044.0 35 267346.0 36 399035.0 37 574933.0 38 470691.0 39 64340.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.128064105615074 19.00106512735086 13.607256154652966 24.263614612381105 2 15.449948930931622 20.132948680659364 40.47166174013623 23.945440648272783 3 19.26532053565177 23.948400678781777 29.739118199286786 27.047160586279666 4 12.841467486108579 16.19130669504428 37.14623682610921 33.82098899273793 5 13.85224006625205 37.097900394589416 34.83645378324105 14.213405755917488 6 32.698744684994644 35.80805662847437 17.583775574236967 13.909423112294025 7 28.735447193340796 32.0495633101399 21.28796713271037 17.927022363808927 8 25.667000724919937 35.82298023240716 20.1481419869298 18.361877055743097 9 26.16186340414726 16.736371539312838 20.3675845799229 36.734180476617 10-11 24.984996093067164 26.21724372397139 27.99967405597108 20.798086126990363 12-13 25.64595427172225 23.530946195920276 28.123063252954 22.700036279403477 14-15 22.732219818295608 25.199304450374804 25.84204207311115 26.226433658218433 16-17 22.4957292410686 28.122852469686276 26.77179650816326 22.60962178108187 18-19 22.444197002199253 27.38578695650998 28.22421829139965 21.94579774989112 20-21 23.10243466317072 26.68414640373121 28.142928974084384 22.070489959013692 22-23 22.749512872789918 26.542045296785304 27.94710905240716 22.761332778017614 24-25 22.55446335529647 26.700243284479143 28.186094869785478 22.559198490438916 26-27 22.461740968359052 26.81408576983349 28.138970513755563 22.5852027480519 28-29 22.43024900591253 27.07700658553887 28.139249186002367 22.353495222546233 30-31 22.64536628199247 26.84552898042597 27.948621654109758 22.560483083471805 32-33 22.447086965879777 26.83662726104406 27.99365420632013 22.722631566756036 34-35 22.818654191623615 26.8413538795724 27.92325346826683 22.416738460537154 36-37 22.845666509724126 26.664718609610215 27.937515249262727 22.55209963140293 38-39 22.591361562308133 26.89027887617101 28.06301486917479 22.455344692346067 40-41 22.795275697457814 26.689675397674506 28.07464577234933 22.440403132518345 42-43 22.48387330062327 26.693825457827142 27.899232362658648 22.92306887889094 44-45 22.624249167099467 26.910289188770243 27.85158606367209 22.613875580458203 46-47 22.791974666133132 26.898422948791882 27.468054666852957 22.841547718222017 48-49 22.646884127884885 26.930191600469605 27.635274808234822 22.78764946341069 50-51 22.622001422531383 27.02886033978091 27.650882477140254 22.698255760547458 52-53 22.736754885364366 27.0043619779507 27.513231106726682 22.74565202995825 54-55 22.63602560861548 26.951473578572944 27.505832294434224 22.90666851837735 56-57 22.532367726226113 27.32254508142112 27.342999023133324 22.80208816921945 58-59 22.575591513072567 26.927434659219156 27.61017755150738 22.886796276200887 60-61 22.6060341206512 26.965591628422704 27.65277850348759 22.77559574743851 62-63 22.556341580054674 26.890135595309296 27.828588977281044 22.72493384735499 64-65 22.448197805433388 26.891549096555188 27.680045833198196 22.980207264813224 66-67 22.37348211310845 27.070767416577148 27.52654529571814 23.029205174596264 68-69 22.43473032738439 26.88242627227457 27.718958266812503 22.963885133528535 70-71 22.503975449782466 26.619914052499542 27.64144712316362 23.23466337455437 72-73 22.427037599694874 26.697050189871547 27.568377361592116 23.307534848841463 74-75 22.273503652287676 26.96891878903067 27.361743071997296 23.395834486684358 76-77 22.44195885593056 26.78445915982868 27.5343744418631 23.239207542377656 78-79 22.35032353876261 26.70545521948881 27.673805437447392 23.270415804301187 80-81 22.309749737134528 26.703481978649585 27.61857974219662 23.36818854201926 82-83 22.450275023767485 26.661839603422514 27.61243718592965 23.27544818688035 84-85 22.371165979547364 26.601957933494642 27.48120127151591 23.545674815442087 86-87 22.183082451344468 26.483094369271715 27.826119139206483 23.507704040177334 88-89 22.41705815139975 26.341322621337632 27.704793063462123 23.536826163800495 90-91 22.274791625135464 26.423542564575577 27.768423469209285 23.533242341079674 92-93 22.208437040598113 26.290901785788577 27.82120411882208 23.67945705479123 94-95 22.243854324322122 26.206361423063235 27.80531040179554 23.744473850819105 96-97 22.206063652387726 26.019654058680285 27.858278172685374 23.916004116246608 98-99 22.08398075646192 25.96398544338083 27.840960614597716 24.111073185559533 100-101 22.47849855508185 25.297621220407024 28.253960600364724 23.969919624146407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1987.0 1 1114.0 2 258.5 3 262.5 4 261.0 5 301.5 6 466.5 7 788.0 8 1171.0 9 1440.5 10 1469.5 11 1520.5 12 1738.0 13 1975.5 14 2210.5 15 2474.0 16 2677.0 17 2814.0 18 2987.0 19 3199.5 20 3442.5 21 3891.5 22 4591.5 23 5799.0 24 7282.0 25 9380.0 26 12819.5 27 17395.5 28 21398.0 29 25227.0 30 30298.5 31 36827.0 32 43850.5 33 50355.5 34 57367.5 35 64013.0 36 72181.0 37 80007.0 38 84767.5 39 88513.0 40 92223.5 41 95492.0 42 97036.0 43 99269.0 44 102830.5 45 105611.5 46 108209.5 47 110911.0 48 115703.5 49 116191.5 50 110633.5 51 108085.5 52 107541.5 53 106325.5 54 104164.5 55 96883.5 56 87833.0 57 80697.5 58 73704.0 59 65110.0 60 53895.0 61 43491.0 62 34430.5 63 25937.0 64 18376.5 65 12963.5 66 9386.0 67 7208.0 68 5504.5 69 4336.0 70 3452.0 71 2190.0 72 1440.5 73 987.5 74 617.0 75 360.0 76 221.0 77 139.0 78 92.0 79 68.5 80 45.0 81 33.0 82 31.5 83 26.5 84 22.5 85 18.0 86 18.0 87 13.0 88 10.0 89 8.0 90 5.0 91 8.0 92 6.0 93 3.5 94 3.5 95 3.0 96 3.0 97 2.0 98 2.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3198340715029363 2 0.08674210898552957 3 0.09711601917585197 4 0.10225228741340574 5 0.11363993475587689 6 0.05687065423554588 7 0.05933741463910789 8 0.060114613122421945 9 0.06345994572451287 10-11 0.06028356931444673 12-13 0.058999502255058296 14-15 0.05697202795076076 16-17 0.05861090301340127 18-19 0.06687286080341373 20-21 0.06819071910120712 22-23 0.08348125447945104 24-25 0.09211491589191804 26-27 0.08655625717430229 28-29 0.08412328800914526 30-31 0.0791559759636163 32-33 0.07138399113047575 34-35 0.31596497470556845 36-37 0.4910711721200656 38-39 0.46484917111781754 40-41 0.44668638047515213 42-43 0.4756454717882019 44-45 0.4854956177832474 46-47 0.4797848984928094 48-49 0.4977449417050451 50-51 0.49323381137798306 52-53 0.4927438384211112 54-55 0.4839750120550243 56-57 0.46309202672075966 58-59 0.4465850067599373 60-61 0.4068803016341105 62-63 0.47562857616899945 64-65 0.4986404095227765 66-67 0.5082202256105823 68-69 0.5206216101052022 70-71 0.5183744927512726 72-73 0.5064630812135245 74-75 0.5078147307497228 76-77 0.48257267566121853 78-79 0.46356510405842916 80-81 0.4705092035506482 82-83 0.47804464971495403 84-85 0.4798693765888218 86-87 0.4799707503040367 88-89 0.4853097659720202 90-91 0.4992317561948632 92-93 0.5055676133957931 94-95 0.5123765479343922 96-97 0.5040639032867724 98-99 0.5062603337830947 100-101 0.48559699149846236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2959347.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.904332137503594 #Duplication Level Percentage of deduplicated Percentage of total 1 72.30022043918342 36.80394434850918 2 14.223539942969701 14.48079602855957 3 5.329725307624045 8.139183217828586 4 2.697288224940682 5.49214622691832 5 1.507654998448916 3.837308539490554 6 0.9032477753715789 2.758753485598565 7 0.6344324991188315 2.2606753857780015 8 0.4233959259307495 1.7242149471395791 9 0.30486206573738134 1.3966919865379002 >10 1.5135977672090686 13.715329902117182 >50 0.10761531217275913 3.781565971099761 >100 0.05261907185705538 4.68958001894824 >500 0.0014672121330531562 0.4872677379231382 >1k 3.3345730287850526E-4 0.4325422035514016 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4379 0.14797183297531516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3567 0.1205333473904885 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.758247680991786E-5 0.0 3 0.0 0.0 0.0 6.758247680991786E-5 0.0 4 0.0 0.0 0.0 1.0137371521487679E-4 0.0 5 0.0 0.0 0.0 1.3516495361983572E-4 6.758247680991786E-5 6 0.0 0.0 0.0 1.3516495361983572E-4 1.3516495361983572E-4 7 0.0 0.0 0.0 1.6895619202479467E-4 1.3516495361983572E-4 8 0.0 0.0 0.0 2.0274743042975358E-4 2.0274743042975358E-4 9 0.0 0.0 0.0 2.3653866883471253E-4 2.3653866883471253E-4 10-11 0.0 0.0 0.0 2.3653866883471253E-4 2.3653866883471253E-4 12-13 0.0 0.0 0.0 4.0549486085950716E-4 4.7307733766942505E-4 14-15 0.0 0.0 0.0 5.237641952768634E-4 6.251379104917403E-4 16-17 0.0 0.0 0.0 6.082422912892608E-4 7.096160065041376E-4 18-19 0.0 0.0 0.0 0.0010306327713512475 7.096160065041376E-4 20-21 0.0 0.0 0.0 0.0013009626785909188 7.603028641115759E-4 22-23 0.0 0.0 0.0 0.0014361276322107546 8.278853409214938E-4 24-25 0.0 0.0 0.0 0.0015881882050330699 9.461546753388501E-4 26-27 0.0 0.0 0.0 0.002314699830739687 0.0010644240097562063 28-29 0.0 0.0 0.0 0.004477339088657059 0.0011151108673636448 30-31 0.0 0.0 0.0 0.011877620299343064 0.0011489021057686038 32-33 0.0 0.0 0.0 0.027827084826483682 0.0011826933441735625 34-35 0.0 0.0 0.0 0.053728069063884706 0.0013685451554008368 36-37 0.0 0.0 0.0 0.09358483476253376 0.001385440774603316 38-39 0.0 0.0 0.0 0.150996148812559 0.0015543969666281109 40-41 0.0 0.0 0.0 0.2185448343840719 0.0016557706818429877 42-43 0.0 0.0 0.0 0.2911453100971262 0.001740248777855385 44-45 0.0 0.0 0.0 0.3817227246416186 0.0017909356354628234 46-47 0.0 0.0 0.0 0.4889085328621483 0.0019429962082851385 48-49 0.0 0.0 0.0 0.606349981938583 0.0021626392579173715 50-51 0.0 0.0 0.0 0.7330164390995717 0.0021626392579173715 52-53 0.0 0.0 0.0 0.8590239671116635 0.0024160735459545636 54-55 0.0 0.0 0.0 1.0060327497924373 0.002652612214789276 56-57 0.0 0.0 0.0 1.1810713647301245 0.002686403453194235 58-59 0.0 0.0 0.0 1.375539941750663 0.002720194691599194 60-61 0.0 0.0 0.0 1.5840656739476646 0.00283846402601655 62-63 0.0 0.0 0.0 1.8038438885335177 0.002973628979636386 64-65 0.0 0.0 0.0 2.0431365432982345 0.002973628979636386 66-67 0.0 0.0 0.0 2.3148181000741044 0.0029905245988388653 68-69 0.0 3.379123840495893E-5 0.0 2.6266436480750652 0.0030412114564463038 70-71 0.0 3.379123840495893E-5 0.0 2.965214961273551 0.0030750026948512628 72-73 1.6895619202479465E-5 3.379123840495893E-5 0.0 3.3200398601448224 0.0033960194596983724 74-75 3.379123840495893E-5 3.379123840495893E-5 0.0 3.6904594155399826 0.0034973931749132494 76-77 3.379123840495893E-5 3.379123840495893E-5 0.0 4.099113757190353 0.003581871270925647 78-79 3.379123840495893E-5 3.379123840495893E-5 0.0 4.554906876415641 0.0036325581285330854 80-81 3.379123840495893E-5 3.379123840495893E-5 0.0 5.049069946849761 0.003733931843747962 82-83 3.379123840495893E-5 3.379123840495893E-5 0.0 5.564808723005447 0.0040042617509876335 84-85 3.379123840495893E-5 3.379123840495893E-5 0.0 6.091073469924277 0.004021157370190113 86-87 3.379123840495893E-5 3.379123840495893E-5 0.0 6.667450623397661 0.004054948608595072 88-89 3.379123840495893E-5 3.379123840495893E-5 0.0 7.290307625297068 0.004088739847000031 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9385 0.0 27.411505 1 TACATGG 8230 0.0 25.210947 2 GTACATG 8615 0.0 25.013151 1 GGTATCA 7140 0.0 24.862297 1 ATCAACG 10750 0.0 23.531395 3 TCAACGC 10800 0.0 23.37859 4 ACATGGG 8290 0.0 23.371342 3 TATCAAC 10875 0.0 23.21736 2 CAACGCA 10855 0.0 23.129215 5 AACGCAG 11210 0.0 22.597345 6 ACGCAGA 12165 0.0 20.434143 7 CGCAGAG 12260 0.0 19.812357 8 CATGGGG 4820 0.0 19.36128 4 GAGTACT 5330 0.0 19.188917 12-13 GTGTAGC 1840 0.0 18.830978 1 GTATAGG 900 0.0 18.45837 1 GTACTTT 5790 0.0 17.419365 14-15 CATGGGA 3965 0.0 17.084715 4 GTAGCCC 1665 0.0 16.78621 3 TAGCCCT 2205 0.0 16.757177 4 >>END_MODULE