FastQCFastQC Report
Fri 27 May 2016
SRR938959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959347
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43010.14533611637972838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37210.12573719810485218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.027.3426191
GTATCAA102150.027.2293531
ATCAACG117350.023.331663
CAACGCA118500.023.0251455
TCAACGC118600.023.0057324
TATCAAC119850.023.0033442
TACATGG82750.022.478692
AACGCAG123050.022.2508756
GTACATG86900.022.0327721
GTATAGG7500.021.5913851
ATACCGA2205.707807E-821.569016
ACATGGG84500.021.0023463
ACGCAGA133100.020.0360057
CGCAGAG133950.019.6608898
GAGTACT63700.019.55531312-13
GTACTTT66150.018.50822414-15
CATACCG1801.2437903E-418.4534855
CATGGGG49450.017.4645734
AGTACTT69450.017.38963312-13
GCAGAGT148200.017.1306239
Produced by FastQC (version 0.11.5)