##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938959_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2959347 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14690571940364 33.0 31.0 34.0 30.0 34.0 2 32.386366992447996 34.0 31.0 34.0 31.0 34.0 3 32.48669554465901 34.0 31.0 34.0 30.0 34.0 4 36.0478298084003 37.0 35.0 37.0 35.0 37.0 5 35.96379370178624 37.0 35.0 37.0 35.0 37.0 6 35.99605115588 37.0 35.0 37.0 35.0 37.0 7 35.9269365843208 37.0 35.0 37.0 35.0 37.0 8 35.88769177794966 37.0 35.0 37.0 35.0 37.0 9 37.572549619899256 39.0 37.0 39.0 35.0 39.0 10-11 37.475030640205425 39.0 37.0 39.0 34.5 39.0 12-13 37.38557272938929 39.0 37.0 39.0 34.0 39.0 14-15 38.719483386030774 40.0 38.0 41.0 34.0 41.0 16-17 38.7951330141413 40.0 38.0 41.0 34.0 41.0 18-19 38.80784510907305 40.0 38.0 41.0 34.0 41.0 20-21 38.77215041020874 40.0 38.0 41.0 34.0 41.0 22-23 38.70087657851546 40.0 38.0 41.0 34.0 41.0 24-25 38.634265430853496 40.0 38.0 41.0 34.0 41.0 26-27 38.4800455978971 40.0 38.0 41.0 34.0 41.0 28-29 38.32794278602678 40.0 38.0 41.0 34.0 41.0 30-31 38.15908678502386 40.0 38.0 41.0 33.0 41.0 32-33 38.10574528772733 40.0 38.0 41.0 33.0 41.0 34-35 38.036585604864854 40.0 38.0 41.0 33.0 41.0 36-37 37.928663992428056 40.0 37.0 41.0 33.0 41.0 38-39 37.80193502147602 40.0 37.0 41.0 33.0 41.0 40-41 37.64627652654454 40.0 37.0 41.0 32.5 41.0 42-43 37.49291279461313 40.0 37.0 41.0 32.0 41.0 44-45 37.29423856006072 40.0 37.0 41.0 31.5 41.0 46-47 37.060943512200495 40.0 36.0 41.0 31.0 41.0 48-49 36.79305535984797 39.0 36.0 41.0 30.5 41.0 50-51 36.56532336356635 39.0 35.5 41.0 30.0 41.0 52-53 36.311716233344725 39.0 35.0 40.0 30.0 41.0 54-55 36.012792855991535 38.5 35.0 40.0 29.0 41.0 56-57 35.88052229089728 38.0 35.0 40.0 29.0 41.0 58-59 35.60523166090357 38.0 34.5 40.0 28.0 41.0 60-61 35.248327080264666 38.0 34.0 40.0 28.0 41.0 62-63 34.81867249768277 37.0 34.0 40.0 27.0 41.0 64-65 34.40710653397523 37.0 33.5 39.5 26.0 41.0 66-67 33.951741887652915 36.0 33.0 39.0 26.0 40.5 68-69 33.45458643410184 36.0 32.5 39.0 25.5 40.0 70-71 32.93171686186176 35.0 32.0 38.0 25.0 40.0 72-73 32.4193798496763 35.0 31.0 37.5 23.5 39.5 74-75 31.88321393199243 35.0 31.0 37.0 22.0 39.0 76-77 29.96400084207766 32.5 28.5 35.0 20.0 37.0 78-79 30.936156354763398 34.0 30.5 36.0 20.0 38.0 80-81 30.933351681975786 34.0 31.0 35.5 20.0 37.0 82-83 30.512148963943737 34.0 30.0 35.0 18.5 37.0 84-85 30.047450332793012 34.0 30.0 35.0 17.0 36.0 86-87 29.32808622983381 34.0 29.0 35.0 7.5 36.0 88-89 28.618960703155125 33.0 28.5 35.0 3.5 35.0 90-91 28.025926496622397 33.0 27.0 35.0 2.0 35.0 92-93 27.59562464286885 33.0 26.5 34.5 2.0 35.0 94-95 27.0156325027109 32.0 25.5 34.0 2.0 35.0 96-97 26.1368266377684 32.0 24.5 34.0 2.0 35.0 98-99 24.854597314880614 32.0 12.5 34.0 2.0 35.0 100-101 19.213314964416135 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16834361549795318 1101 2 0.18881119426229986 1101 3 0.20411083526121843 1101 4 0.14716665502204052 1101 5 0.1880724231676325 1101 6 0.19313621534865888 1101 7 0.21882181128079026 1101 8 0.1740511643837479 1101 9 0.29397810170213035 1101 10-11 0.33373739507131717 1101 12-13 0.2719765665896645 1101 14-15 0.3491390162100103 1101 16-17 0.2899457685010489 1101 18-19 0.2726908897018774 1101 20-21 0.30133550257820474 1101 22-23 0.3126356237657717 1101 24-25 0.2265005737444099 1101 26-27 0.22406178347681305 1101 28-29 0.32731930650436425 1101 30-31 0.11991949214067432 1101 32-33 0.27895305820007366 1101 34-35 0.4013007279546983 1101 36-37 0.3219127274024416 1101 38-39 0.24480775867795757 1101 40-41 0.36746738016412195 1101 42-43 0.40560299622674023 1101 44-45 0.4445610077174109 1101 46-47 0.4413560252336026 1101 48-49 0.38670610228836466 1101 50-51 0.25654871494861453 1101 52-53 0.307897811849152 1101 54-55 0.37231611432132183 1101 56-57 0.3804628148577862 1101 58-59 0.46214113703562276 1101 60-61 0.3856358222381928 1101 62-63 0.2899776236147247 1101 64-65 0.3695583752412901 1101 66-67 0.30703373297655645 1101 68-69 0.3032361241035417 1101 70-71 0.2670100326541558 1101 72-73 0.2761329293906556 1101 74-75 0.2545078571457289 1101 76-77 -0.09116771957950576 1101 78-79 0.29397848327617027 1101 80-81 0.40590367133479077 1101 82-83 0.3646613892695463 1101 84-85 0.5147685265125048 1101 86-87 0.6173396254761414 1101 88-89 0.5334679144561036 1101 90-91 0.6052571809486587 1101 92-93 0.4888935813723343 1101 94-95 0.384570944220183 1101 96-97 0.3758643011012488 1101 98-99 0.7614221268360843 1101 100-101 0.9700817330376985 1102 1 0.05709301394542621 1102 2 0.1187088780235186 1102 3 0.15153619043875466 1102 4 0.09665716618476239 1102 5 0.11410970896262285 1102 6 0.12535223024623576 1102 7 0.17665873300573054 1102 8 0.11825429036453983 1102 9 0.17645252264243538 1102 10-11 0.22583247175618482 1102 12-13 0.23350022068126464 1102 14-15 0.30136109894178986 1102 16-17 0.37538715113030463 1102 18-19 0.23863673951252196 1102 20-21 0.251698526451996 1102 22-23 0.25835047740542905 1102 24-25 0.22941941123107057 1102 26-27 0.28773219546182816 1102 28-29 0.22289711078531838 1102 30-31 0.20642274575521924 1102 32-33 0.22865385120323367 1102 34-35 0.21329934699932807 1102 36-37 0.23806258198366237 1102 38-39 0.25459998766547187 1102 40-41 0.275544922900508 1102 42-43 0.36044889416159265 1102 44-45 0.26592501499881394 1102 46-47 0.27864182022450024 1102 48-49 0.2832317441187655 1102 50-51 0.22219745011903314 1102 52-53 0.20893875926822858 1102 54-55 0.2566788871123151 1102 56-57 0.256109876142915 1102 58-59 0.254852412745457 1102 60-61 0.3187431810564334 1102 62-63 0.2704371051287495 1102 64-65 0.27066941660718413 1102 66-67 0.037622940601103494 1102 68-69 -0.022974827291008637 1102 70-71 0.20923692780617387 1102 72-73 0.30614883130097326 1102 74-75 0.2978939178659239 1102 76-77 -0.0011262909184033276 1102 78-79 0.3098040225810905 1102 80-81 0.3918158342038325 1102 82-83 0.3170717840972408 1102 84-85 0.5064409471598807 1102 86-87 0.6159126382604398 1102 88-89 0.5075111843910278 1102 90-91 0.6837864634795743 1102 92-93 0.6991827450573957 1102 94-95 0.7778733106755205 1102 96-97 0.9646607775726963 1102 98-99 1.014112897451028 1102 100-101 1.0135221950300384 1103 1 0.08937077305882468 1103 2 0.10755504622688505 1103 3 0.09964382997571164 1103 4 0.11799594122095414 1103 5 0.15668398281465556 1103 6 0.12306356284128839 1103 7 0.10701696461821086 1103 8 0.14133217058900982 1103 9 0.18798669296939607 1103 10-11 0.19555719293112617 1103 12-13 0.15313970786713327 1103 14-15 0.2237079577271146 1103 16-17 0.1727048727490441 1103 18-19 0.2291247507609171 1103 20-21 0.17459100719658238 1103 22-23 0.1365950719944209 1103 24-25 0.10237016630881612 1103 26-27 0.21092040289357783 1103 28-29 0.18028778938059986 1103 30-31 0.3264008876957334 1103 32-33 0.2693842919728695 1103 34-35 0.14539149925087003 1103 36-37 0.21390527861287012 1103 38-39 0.18359240163305657 1103 40-41 0.34110811011250064 1103 42-43 0.306746711258981 1103 44-45 0.3306935145073169 1103 46-47 0.18595369367172765 1103 48-49 0.1465224397193623 1103 50-51 0.2617346150416253 1103 52-53 0.21112892023811725 1103 54-55 0.403324691152946 1103 56-57 0.4498004871316681 1103 58-59 0.40401541912037686 1103 60-61 0.3294030290039771 1103 62-63 0.3309806117919365 1103 64-65 0.31837846800058145 1103 66-67 0.16761804638664302 1103 68-69 0.2107935934123475 1103 70-71 0.14659907064257283 1103 72-73 0.26467928119541284 1103 74-75 0.36442101646774816 1103 76-77 0.08234391451417267 1103 78-79 0.28511665089868643 1103 80-81 0.21630722992595608 1103 82-83 0.300985786942519 1103 84-85 0.46252615626311666 1103 86-87 0.6560978718347528 1103 88-89 0.6029353012549166 1103 90-91 0.5671334840228326 1103 92-93 0.6328008138335512 1103 94-95 0.8500239861185932 1103 96-97 0.6419261145061839 1103 98-99 0.7823542154049115 1103 100-101 1.0748888007264092 1104 1 0.009140781605822212 1104 2 0.020865855354053053 1104 3 0.013989892031794682 1104 4 0.055155557397149835 1104 5 0.014569204677819414 1104 6 0.041078284592728664 1104 7 0.056445342421937994 1104 8 0.03175235691819722 1104 9 0.08671450151337012 1104 10-11 0.08106949335082447 1104 12-13 0.09923656740020448 1104 14-15 0.11915605830419196 1104 16-17 0.08473635544011415 1104 18-19 -0.008286877268602666 1104 20-21 0.09973213070268372 1104 22-23 0.04853811633518745 1104 24-25 0.1457031878271806 1104 26-27 -0.02402625291811944 1104 28-29 0.026630478933121537 1104 30-31 -0.08654935984313994 1104 32-33 -0.032094010539118756 1104 34-35 0.0729361851272543 1104 36-37 0.0374636211678947 1104 38-39 0.07292096324422204 1104 40-41 0.00229479890850115 1104 42-43 0.0014494923625605338 1104 44-45 -0.002693126930928713 1104 46-47 0.09924659726684837 1104 48-49 -0.10318225516560631 1104 50-51 0.08607446939271313 1104 52-53 -0.01631865365760632 1104 54-55 0.160464062504019 1104 56-57 -0.002518102255542942 1104 58-59 -0.003984935994665761 1104 60-61 -0.02736830536937873 1104 62-63 -0.08946408809569562 1104 64-65 0.051689729191366496 1104 66-67 0.025686068073177637 1104 68-69 0.09649972291849451 1104 70-71 -0.010793755897481105 1104 72-73 0.006325873922449432 1104 74-75 0.11892786611670303 1104 76-77 -0.13552222536220526 1104 78-79 0.008472300107662534 1104 80-81 0.036543721835990084 1104 82-83 0.13032400800952715 1104 84-85 0.15615794145555384 1104 86-87 0.19260241786551546 1104 88-89 0.3498927564852998 1104 90-91 0.4986052002946302 1104 92-93 0.25616829567168864 1104 94-95 0.38255323170746536 1104 96-97 0.5284340442059303 1104 98-99 0.4880348577818765 1104 100-101 0.6000918923513971 1105 1 0.00704181894636946 1105 2 0.041723724573245136 1105 3 0.05076691821608392 1105 4 0.007639840719292579 1105 5 -0.051120803256814895 1105 6 -0.027045851288534095 1105 7 -0.008644260554476091 1105 8 0.07100265347180823 1105 9 0.018207433320633015 1105 10-11 0.054422857874250496 1105 12-13 0.11117381214517508 1105 14-15 0.018536347506987738 1105 16-17 -0.030490843103528675 1105 18-19 0.020180942352496345 1105 20-21 0.05859974704855375 1105 22-23 0.03150678382097283 1105 24-25 -0.051805496483211755 1105 26-27 -0.02473917575579776 1105 28-29 0.10589700300637617 1105 30-31 0.01730437752845404 1105 32-33 0.17094092042426468 1105 34-35 0.22699737733910297 1105 36-37 0.18824580619081388 1105 38-39 0.16216270153930878 1105 40-41 0.32696693136115584 1105 42-43 0.340035561906042 1105 44-45 0.21341039747934332 1105 46-47 0.22840538016306766 1105 48-49 0.22090924256944078 1105 50-51 0.21987481384603313 1105 52-53 0.1550432240110453 1105 54-55 0.26095838486243395 1105 56-57 0.3405856662973008 1105 58-59 0.27032542759200595 1105 60-61 0.2528377031915312 1105 62-63 0.320887755844133 1105 64-65 0.16028222074589138 1105 66-67 0.3509437446320405 1105 68-69 0.28940897353349726 1105 70-71 0.5319176596252078 1105 72-73 0.4126788881543675 1105 74-75 0.32345571561873143 1105 76-77 0.14680570056706088 1105 78-79 0.260245916257162 1105 80-81 0.3019079852021349 1105 82-83 0.15995163751787445 1105 84-85 0.32879227957709034 1105 86-87 0.33333375754478567 1105 88-89 0.5435140674778154 1105 90-91 0.5590592789350772 1105 92-93 0.5831418698923585 1105 94-95 0.6182365257214641 1105 96-97 0.5118780599265911 1105 98-99 0.7572851012610151 1105 100-101 1.022211872501547 1106 1 0.06987315612539646 1106 2 0.08700474501339528 1106 3 0.020326871097068988 1106 4 0.06997091753036955 1106 5 0.11602632233153543 1106 6 0.12132318363110528 1106 7 0.10391533337262615 1106 8 0.1185440721990858 1106 9 0.06743138515003722 1106 10-11 0.16348884471936032 1106 12-13 0.18247848309569292 1106 14-15 0.10603274532899576 1106 16-17 0.020803413128881232 1106 18-19 0.04116963277752461 1106 20-21 0.10429748260572325 1106 22-23 0.09689554098108744 1106 24-25 0.19920564119835404 1106 26-27 0.1093159413592204 1106 28-29 0.1729264574470406 1106 30-31 0.15508806484423587 1106 32-33 0.250998504715227 1106 34-35 0.16359057642705466 1106 36-37 0.139121956991886 1106 38-39 0.3750634280417344 1106 40-41 0.44140054258882344 1106 42-43 0.3031409173967745 1106 44-45 0.47446857834154343 1106 46-47 0.41063349787971504 1106 48-49 0.5077185723368345 1106 50-51 0.33114647115124995 1106 52-53 0.3994421532162349 1106 54-55 0.5955879545727925 1106 56-57 0.5331120949084607 1106 58-59 0.4459711653938925 1106 60-61 0.4250862711677925 1106 62-63 0.6300662317563024 1106 64-65 0.5955105103559433 1106 66-67 0.5927708451441802 1106 68-69 0.48608460562093825 1106 70-71 0.7071581840012087 1106 72-73 0.6855745842468224 1106 74-75 0.7130024478745263 1106 76-77 0.489141276940817 1106 78-79 0.5449289498724355 1106 80-81 0.6088888797556393 1106 82-83 0.5321911052549986 1106 84-85 0.7392655172905052 1106 86-87 0.8829151465446188 1106 88-89 0.933351249032647 1106 90-91 1.0465108075224663 1106 92-93 1.14396496124359 1106 94-95 1.1173447022863243 1106 96-97 1.1175499103591022 1106 98-99 1.2642991105735923 1106 100-101 1.3806872285483998 1107 1 0.08848601730554151 1107 2 0.10348398974540629 1107 3 0.07252038410216954 1107 4 0.0552983365772306 1107 5 0.034000758979146894 1107 6 0.0017478753701638539 1107 7 0.053013515379049636 1107 8 0.03501218247126303 1107 9 -0.04060364151650475 1107 10-11 0.03493002962121494 1107 12-13 0.07596650891201051 1107 14-15 0.0795290682713059 1107 16-17 0.12126847216837433 1107 18-19 0.05369492338448367 1107 20-21 0.09897113305301986 1107 22-23 0.20763629022279417 1107 24-25 0.21467277329846013 1107 26-27 0.12954012491762512 1107 28-29 0.18671939165556495 1107 30-31 0.23929656834841495 1107 32-33 0.32280722446233767 1107 34-35 0.32641056395450363 1107 36-37 0.3590871178530861 1107 38-39 0.2459420297098589 1107 40-41 0.3077745715589373 1107 42-43 0.34207184809599767 1107 44-45 0.15147352585086793 1107 46-47 0.20347687265635273 1107 48-49 0.05905661209564528 1107 50-51 0.018548953061419127 1107 52-53 0.020084269701250435 1107 54-55 0.024714065515965444 1107 56-57 0.16777606130582967 1107 58-59 0.20717130877447687 1107 60-61 0.2870128647995571 1107 62-63 0.3401843547012575 1107 64-65 0.21703276193906618 1107 66-67 0.17840448625416627 1107 68-69 0.22325616957790828 1107 70-71 0.2837707150543878 1107 72-73 0.26034663304115213 1107 74-75 0.19678789074034242 1107 76-77 0.2253542029743052 1107 78-79 0.369446200227344 1107 80-81 0.3397837582459644 1107 82-83 0.2597714064421375 1107 84-85 0.38958402144419324 1107 86-87 0.44707881431382646 1107 88-89 0.5987276450521719 1107 90-91 0.5658565652200132 1107 92-93 0.651897775522265 1107 94-95 0.8231940051861173 1107 96-97 0.7749328141773937 1107 98-99 0.8366345195720903 1107 100-101 1.002446697844885 1108 1 0.06884965313227553 1108 2 0.004039537645169844 1108 3 0.10904539458513085 1108 4 0.04196331678263476 1108 5 0.04418153843923278 1108 6 0.0865656391225329 1108 7 0.11206976312742967 1108 8 0.07296272847129615 1108 9 0.025783298973721003 1108 10-11 0.09919429285091752 1108 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-0.9797905489661289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 14.0 5 51.0 6 110.0 7 291.0 8 735.0 9 1394.0 10 2209.0 11 3492.0 12 4924.0 13 6634.0 14 8258.0 15 10710.0 16 13523.0 17 17888.0 18 18171.0 19 15130.0 20 16147.0 21 17530.0 22 19453.0 23 22148.0 24 25630.0 25 31057.0 26 37578.0 27 45270.0 28 54979.0 29 67419.0 30 81273.0 31 98682.0 32 122139.0 33 155199.0 34 208426.0 35 296501.0 36 456448.0 37 662798.0 38 416424.0 39 20700.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28139859039164 19.620843370101742 14.118302757444171 23.97945528206245 2 15.857180655056673 20.715684912921667 39.82253517414483 23.60459925787682 3 19.220084701118186 24.270658358076968 29.973065003867404 26.536191936937435 4 13.202777504631936 16.779073221220763 36.85235290082576 33.16579637332155 5 13.750364522984293 37.398959973264375 34.83741514597646 14.013260357774874 6 32.62598133980233 36.29266861912443 17.268133814655734 13.813216226417518 7 28.737758701497324 32.22981286074259 21.1683523425945 17.86407609516559 8 25.601357326464253 36.409011852952695 19.84839898801999 18.14123183256306 9 25.98637469685035 16.88233924578632 20.2075660610263 36.92371999633703 10-11 24.92046387260433 26.54376117434015 27.832998293204547 20.702776659850976 12-13 25.602117629328365 23.64764929560474 28.123940855871243 22.626292219195655 14-15 22.59751152039474 25.387023424661837 25.775711084686094 26.23975397025733 16-17 22.452245011610962 28.38143975950842 26.695738005833867 22.47057722304675 18-19 22.37592607391966 27.572854640122635 28.188297064762363 21.862922221195337 20-21 23.011563023869794 26.850974218298834 28.155839784925522 21.98162297290585 22-23 22.712916058846766 26.64707788576331 27.998778108819277 22.64122794657065 24-25 22.52991032478122 26.87088633772462 28.14988514354185 22.449318193952312 26-27 22.51055722765867 26.951942438652853 28.11456378721387 22.422936546474613 28-29 22.42525124630535 27.176502113473006 28.161939103457623 22.236307536764023 30-31 22.69718623736926 26.961235029214215 27.938849347508082 22.402729385908447 32-33 22.50219389615344 26.926362471180298 28.010486772926598 22.560956859739665 34-35 22.79585165899169 26.925993742408664 27.999983778117087 22.27817082048256 36-37 22.84293572052886 26.703905195367927 28.019004459430136 22.43415462467308 38-39 22.58480029996362 26.982785379848874 28.102005204078168 22.33040911610934 40-41 22.80940085070893 26.789558860751576 28.11110784443929 22.289932444100206 42-43 22.51508428784082 26.784879722763378 27.904656825059433 22.795379164336367 44-45 22.604954598085385 27.013256382482094 27.87346227973824 22.508326739694283 46-47 22.828624635937217 26.972055319633526 27.509629775309186 22.689690269120074 48-49 22.6856207295067 27.060464440434433 27.599703980049068 22.6542108500098 50-51 22.736371087388356 27.12095277148552 27.603579803431494 22.539096337694634 52-53 22.78031268384546 27.123753990322864 27.43275796991701 22.663175355914667 54-55 22.715788314111187 27.076361778459912 27.37754308636331 22.830306821065594 56-57 22.555060288638 27.462207034186935 27.258209327936196 22.724523349238872 58-59 22.612242442899458 27.05426027841874 27.520009245368744 22.813488033313064 60-61 22.65822774116536 27.09827738124102 27.53255201183387 22.710942865759755 62-63 22.621000096137422 27.00420757505435 27.697326281453538 22.67746604735469 64-65 22.447452077291338 27.032809029769485 27.590183226059345 22.929555666879835 66-67 22.412793030707768 27.199839149384097 27.350518329282497 23.036849490625634 68-69 22.44319319425026 26.995982951833437 27.580841510342523 22.97998234357378 70-71 22.526467423286668 26.817906189054302 27.45480377333434 23.200822614324686 72-73 22.454429980668035 26.89789335282412 27.30872722935161 23.338949437156238 74-75 22.32664841263968 27.0974306156054 27.08132909050544 23.49459188124948 76-77 22.459800624898957 26.92791609929927 27.33018042790494 23.282102847896834 78-79 22.396822025629184 26.872543607851128 27.38509014212585 23.345544224393837 80-81 22.35822096879001 26.86086192838203 27.31587877165896 23.465038331169 82-83 22.422551735661912 26.83715813609525 27.30104433529497 23.43924579294787 84-85 22.390069275558307 26.79410720156954 27.123673817920952 23.692149704951206 86-87 22.22665288763545 26.65801164926785 27.451472798842886 23.663862664253816 88-89 22.390652921988504 26.499768457752154 27.36501867525254 23.744559945006806 90-91 22.273023391736494 26.525401837966978 27.393723612276926 23.807851158019602 92-93 22.1727969041819 26.515461012175994 27.326079030272552 23.985663053369542 94-95 22.213051547116358 26.395966359748478 27.355378547446165 24.035603545688996 96-97 22.17879484899878 26.23592299247097 27.30335442244522 24.28192773608502 98-99 22.006256785703062 26.30827003389599 27.19976738111482 24.485705799286126 100-101 22.327563062721346 25.502464995942503 27.920761265493553 24.2492106758426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 426.0 1 287.5 2 174.0 3 200.5 4 168.0 5 155.5 6 262.5 7 555.5 8 897.5 9 1028.5 10 950.5 11 902.0 12 1000.5 13 1116.5 14 1270.5 15 1427.0 16 1554.5 17 1748.0 18 1988.0 19 2271.0 20 2674.0 21 3233.5 22 4052.5 23 5456.5 24 7107.5 25 9226.0 26 12755.0 27 17412.5 28 21450.0 29 25636.0 30 30790.0 31 37295.5 32 44335.0 33 50683.5 34 57792.0 35 64922.5 36 73252.5 37 80607.5 38 84852.0 39 88524.0 40 92761.5 41 96264.5 42 97286.0 43 98891.0 44 102646.5 45 106060.5 46 108509.5 47 111401.5 48 116395.5 49 117057.5 50 111507.0 51 109176.5 52 108980.0 53 107991.0 54 106167.0 55 98534.0 56 89304.0 57 81543.5 58 74212.5 59 65597.5 60 53895.5 61 43324.0 62 34186.5 63 25307.0 64 17548.0 65 12160.5 66 8726.0 67 6698.5 68 5123.5 69 3965.0 70 3101.0 71 1927.5 72 1155.0 73 765.5 74 441.5 75 215.0 76 127.5 77 69.0 78 39.5 79 21.5 80 13.0 81 8.0 82 4.5 83 3.5 84 1.5 85 0.0 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3864366023991104 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001013737152148768 16-17 0.0025343428803719197 18-19 4.223904800619866E-4 20-21 0.0 22-23 0.0 24-25 3.379123840495893E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012874461832289354 36-37 0.02274150344653736 38-39 0.010863883147194298 40-41 0.005676928052033101 42-43 0.004798355853504168 44-45 0.0018754137314752207 46-47 0.0012164845825785215 48-49 0.0029398377412314268 50-51 7.603028641115759E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.292590561363705E-4 60-61 0.0015037101090206726 62-63 0.0014023363938057956 64-65 8.278853409214938E-4 66-67 0.002686403453194235 68-69 0.0028553596452190297 70-71 5.06868576074384E-5 72-73 0.0 74-75 0.0 76-77 0.09154046483903375 78-79 0.0034973931749132494 80-81 2.027474304297536E-4 82-83 1.6895619202479465E-5 84-85 2.027474304297536E-4 86-87 0.09057741454449242 88-89 0.43254474720267677 90-91 0.3839191551379409 92-93 0.0 94-95 0.0011826933441735625 96-97 0.0 98-99 0.0 100-101 3.885992416570277E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2959347.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.662802246365985 #Duplication Level Percentage of deduplicated Percentage of total 1 70.16742087748887 34.14543326298773 2 15.221179327675419 14.814104791582858 3 5.761498630294748 8.411120054662256 4 2.8565993994616847 5.560405266923672 5 1.617099216224116 3.93462896859338 6 1.02628728919932 2.996520924135932 7 0.6777841764674295 2.3088014141605386 8 0.4705202364875847 1.8317466576886952 9 0.3449518517858799 1.5107691373176622 >10 1.6786758136712099 14.505140760691585 >50 0.11630405339975157 3.90358878899153 >100 0.05966304880720368 5.095745997657475 >500 0.0015989592362401378 0.5038356104685664 >1k 4.1711980066743847E-4 0.47815836413815976 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4301 0.14533611637972838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3721 0.12573719810485218 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.758247680991786E-5 2 0.0 0.0 0.0 0.0 6.758247680991786E-5 3 0.0 0.0 0.0 3.379123840495893E-5 6.758247680991786E-5 4 0.0 0.0 0.0 3.379123840495893E-5 6.758247680991786E-5 5 0.0 0.0 0.0 6.758247680991786E-5 6.758247680991786E-5 6 0.0 0.0 0.0 6.758247680991786E-5 2.0274743042975358E-4 7 0.0 0.0 0.0 2.0274743042975358E-4 2.3653866883471253E-4 8 0.0 0.0 0.0 2.3653866883471253E-4 2.7032990723967144E-4 9 0.0 0.0 0.0 4.392860992644661E-4 3.041211456446304E-4 10-11 0.0 0.0 0.0 5.406598144793429E-4 3.3791238404958933E-4 12-13 0.0 0.0 0.0 8.109897217190143E-4 4.899729568719045E-4 14-15 0.0 0.0 0.0 8.954678177314117E-4 5.406598144793429E-4 16-17 0.0 0.0 0.0 9.79945913743809E-4 6.082422912892608E-4 18-19 0.0 0.0 0.0 0.0013009626785909188 8.109897217190143E-4 20-21 0.0 0.0 0.0 0.0015375013474256314 8.109897217190143E-4 22-23 0.0 0.0 0.0 0.001706457539450426 9.123634369338912E-4 24-25 0.0 0.0 0.0 0.0018585181122727412 0.001047528390553727 26-27 0.0 0.0 0.0 0.002618820976384317 0.0010813196289586858 28-29 0.0 0.0 0.0 0.004849042711111607 0.0011151108673636448 30-31 0.0 0.0 0.0 0.012452071352227367 0.0011151108673636448 32-33 0.0 0.0 0.0 0.028773239501822534 0.0013009626785909188 34-35 0.0 0.0 0.0 0.055147301076892974 0.001385440774603316 36-37 0.0 0.0 0.0 0.09547714411321147 0.001385440774603316 38-39 0.0 0.0 0.0 0.1537839259809681 0.0015037101090206724 40-41 0.0 0.0 0.0 0.2220084363205802 0.0015206057282231519 42-43 0.0 0.0 0.0 0.2956902316625931 0.0015543969666281109 44-45 0.0 0.0 0.0 0.3877375650777013 0.0016219794434380287 46-47 0.0 0.0 0.0 0.4967312045528963 0.0016557706818429877 48-49 0.0 0.0 0.0 0.615828424311174 0.0017571443970578644 50-51 0.0 0.0 0.0 0.7434579317667039 0.0018754137314752207 52-53 0.0 0.0 0.0 0.8706819443613742 0.0020443699235000155 54-55 0.0 0.0 0.0 1.0194647670584085 0.00221332611552481 56-57 0.0 0.0 0.0 1.1965477519195957 0.002247117353929769 58-59 0.0 0.0 0.0 1.393753419250936 0.002348491069144646 60-61 0.0 0.0 0.0 1.6045600600402725 0.002399177926752084 62-63 0.0 0.0 0.0 1.826788139410485 0.0026695078339917556 64-65 0.0 0.0 0.0 2.0686151370555734 0.002686403453194235 66-67 0.0 0.0 0.0 2.3437602957679515 0.002753985930004153 68-69 0.0 0.0 0.0 2.658694637702169 0.002804672787611591 70-71 0.0 0.0 0.0 3.0006450747411506 0.002906046502826468 72-73 0.0 0.0 0.0 3.3606738243267857 0.0033791238404958933 74-75 0.0 0.0 0.0 3.736635142820359 0.0034129150789008523 76-77 0.0 0.0 0.0 4.150915725665155 0.0034298106981033314 78-79 0.0 1.6895619202479465E-5 0.0 4.610577941687811 0.00348049755571077 80-81 0.0 3.379123840495893E-5 0.0 5.107511893671138 0.003548080032520688 82-83 0.0 3.379123840495893E-5 0.0 5.62561605651517 0.0038184099397603594 84-85 0.0 3.379123840495893E-5 0.0 6.156662263668302 0.0038184099397603594 86-87 0.0 3.379123840495893E-5 0.0 6.738327745952063 0.003835305558962839 88-89 0.0 3.379123840495893E-5 0.0 7.367740248102031 0.0038690967973677975 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7525 0.0 27.342619 1 GTATCAA 10215 0.0 27.229353 1 ATCAACG 11735 0.0 23.33166 3 CAACGCA 11850 0.0 23.025145 5 TCAACGC 11860 0.0 23.005732 4 TATCAAC 11985 0.0 23.003344 2 TACATGG 8275 0.0 22.47869 2 AACGCAG 12305 0.0 22.250875 6 GTACATG 8690 0.0 22.032772 1 GTATAGG 750 0.0 21.591385 1 ATACCGA 220 5.707807E-8 21.56901 6 ACATGGG 8450 0.0 21.002346 3 ACGCAGA 13310 0.0 20.036005 7 CGCAGAG 13395 0.0 19.660889 8 GAGTACT 6370 0.0 19.555313 12-13 GTACTTT 6615 0.0 18.508224 14-15 CATACCG 180 1.2437903E-4 18.453485 5 CATGGGG 4945 0.0 17.464573 4 AGTACTT 6945 0.0 17.389633 12-13 GCAGAGT 14820 0.0 17.130623 9 >>END_MODULE