FastQCFastQC Report
Fri 27 May 2016
SRR938958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976624
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43690.14677701987217734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.11731411155725413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96400.026.0678751
GTACATG86600.024.5830211
TACATGG83800.024.2860222
GGTATCA75700.023.4251561
ACATGGG86000.022.839243
CTATACG1703.2516782E-622.2727094
GTATAGG7300.022.0832421
ATCAACG111150.021.929553
TATCAAC113150.021.625592
TCAACGC114000.021.3813114
CAACGCA113750.021.3454485
AACGCAG118300.020.6017136
CATGGGG49500.020.1747994
GTGTAGC17400.018.802111
ACGCAGA127700.018.677897
CGCAGAG129450.018.1335328
GAGTACT55600.018.13147512-13
CCTATAC7700.017.82543
AGTACTT60700.016.8419912-13
CATGGGA40050.016.5446454
Produced by FastQC (version 0.11.5)