FastQCFastQC Report
Fri 27 May 2016
SRR938958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976624
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.14805363391546936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38320.12873644773407728No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG856.2856247E-428.0346531
GGTATCA75150.027.0796451
GTATCAA101400.027.0255131
TACATGG85450.024.1014272
GTACATG89450.023.6562941
ATCAACG115050.023.3863033
TCAACGC115600.023.2339864
TATCAAC116500.023.1766932
CAACGCA116600.022.9940285
AACGCAG121000.022.1967246
ACATGGG88150.022.1250693
ACGCAGA133650.019.7762577
CGCAGAG134450.019.5527028
GAGTACT62450.018.54210312-13
CATGGGG50150.017.9782664
GTACTTT66150.016.89517414-15
CATGGGA42900.016.813244
AGTACTT68400.016.61693812-13
GCAGAGT148150.016.4959749
TAAGGTG11200.016.1001785
Produced by FastQC (version 0.11.5)