##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938958_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2976624 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.122933565005184 33.0 31.0 34.0 30.0 34.0 2 32.356216640059344 34.0 31.0 34.0 30.0 34.0 3 32.461550064771366 34.0 31.0 34.0 30.0 34.0 4 36.045684305441334 37.0 35.0 37.0 35.0 37.0 5 35.95249181623208 37.0 35.0 37.0 35.0 37.0 6 35.98509082772967 37.0 35.0 37.0 35.0 37.0 7 35.921612874182294 37.0 35.0 37.0 35.0 37.0 8 35.88014105913276 37.0 35.0 37.0 35.0 37.0 9 37.56298712904284 39.0 37.0 39.0 35.0 39.0 10-11 37.47655330333963 39.0 37.0 39.0 34.5 39.0 12-13 37.40536964023673 39.0 37.0 39.0 34.0 39.0 14-15 38.68355123119346 40.0 38.0 41.0 34.0 41.0 16-17 38.666599812404925 40.0 38.0 41.0 34.0 41.0 18-19 38.741473561995065 40.0 38.0 41.0 34.0 41.0 20-21 38.73009708313848 40.0 38.0 41.0 34.0 41.0 22-23 38.671570712323756 40.0 38.0 41.0 34.0 41.0 24-25 38.61970272362247 40.0 38.0 41.0 34.0 41.0 26-27 38.4716616878719 40.0 38.0 41.0 34.0 41.0 28-29 38.29721254683158 40.0 38.0 41.0 34.0 41.0 30-31 38.148198596799595 40.0 38.0 41.0 33.0 41.0 32-33 38.03352724428749 40.0 37.5 41.0 33.0 41.0 34-35 38.04031715124248 40.0 38.0 41.0 33.0 41.0 36-37 37.96351521052038 40.0 37.5 41.0 33.0 41.0 38-39 37.8550824692672 40.0 37.0 41.0 33.0 41.0 40-41 37.71058605319315 40.0 37.0 41.0 33.0 41.0 42-43 37.57470846166664 40.0 37.0 41.0 32.0 41.0 44-45 37.38152400168782 40.0 37.0 41.0 31.5 41.0 46-47 37.15819028537027 40.0 36.5 41.0 31.0 41.0 48-49 36.93738628056483 39.5 36.0 41.0 31.0 41.0 50-51 36.705392753669926 39.0 36.0 41.0 30.5 41.0 52-53 36.48750782765979 39.0 35.5 41.0 30.0 41.0 54-55 36.246851634603495 39.0 35.0 40.0 30.0 41.0 56-57 35.97678527754933 38.5 35.0 40.0 29.0 41.0 58-59 35.674371368368995 38.0 35.0 40.0 28.5 41.0 60-61 35.27827397749934 38.0 34.0 40.0 28.0 41.0 62-63 34.95869767226227 37.0 34.0 40.0 28.0 41.0 64-65 34.700517431828814 37.0 34.0 40.0 27.5 41.0 66-67 34.25084021361113 36.5 33.5 39.0 26.0 41.0 68-69 33.83724968286219 36.0 33.0 39.0 26.0 40.0 70-71 33.38663952853972 35.0 32.5 38.5 26.0 40.0 72-73 32.86882992275813 35.0 32.0 37.5 25.0 39.5 74-75 32.39840184719333 35.0 32.0 37.0 24.5 39.0 76-77 30.398507671778496 33.0 29.5 35.0 21.0 37.5 78-79 31.396138544875 34.0 31.0 36.0 22.0 38.0 80-81 31.406267973381926 34.0 31.0 36.0 22.5 37.0 82-83 31.08468620826816 34.0 31.0 35.0 21.5 37.0 84-85 30.678852619612016 34.0 31.0 35.0 20.0 36.5 86-87 30.09501065636775 34.0 30.0 35.0 17.5 36.0 88-89 29.519615174775183 34.0 29.0 35.0 11.0 35.5 90-91 29.055376157687366 34.0 29.0 35.0 6.5 35.0 92-93 28.693439278860886 34.0 29.0 35.0 2.0 35.0 94-95 28.293134268889858 33.0 29.0 35.0 2.0 35.0 96-97 27.66179403243406 33.0 27.5 35.0 2.0 35.0 98-99 26.735416868237305 33.0 26.0 34.5 2.0 35.0 100-101 22.3622948346852 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21763905633479652 1101 2 0.21245685171473383 1101 3 0.195431589407832 1101 4 0.13189834029812175 1101 5 0.2385797400111258 1101 6 0.15932150300057657 1101 7 0.22026178910560645 1101 8 0.21669417339163743 1101 9 0.2524408504485294 1101 10-11 0.2800849424275782 1101 12-13 0.30747407838680374 1101 14-15 0.3447087452707507 1101 16-17 0.3397898468423435 1101 18-19 0.30726754906906706 1101 20-21 0.37885368263103913 1101 22-23 0.30487183909284 1101 24-25 0.38438442312930476 1101 26-27 0.39874104708417946 1101 28-29 0.4455973353546838 1101 30-31 0.3835018932704415 1101 32-33 0.4427462078539506 1101 34-35 0.45022055724458454 1101 36-37 0.38723303159962086 1101 38-39 0.4521865452954188 1101 40-41 0.5074746868621531 1101 42-43 0.40822960657096985 1101 44-45 0.44784147059641555 1101 46-47 0.5099233577325535 1101 48-49 0.37553532524211164 1101 50-51 0.41057323890159836 1101 52-53 0.3980656516506329 1101 54-55 0.43702673698330585 1101 56-57 0.30549642527451226 1101 58-59 0.38466390948391194 1101 60-61 0.45329469229380237 1101 62-63 0.34444732374691256 1101 64-65 0.4179253114602872 1101 66-67 0.4297717199513542 1101 68-69 0.44473331639201064 1101 70-71 0.4450186967263505 1101 72-73 0.33099520289075457 1101 74-75 0.25909240735911254 1101 76-77 0.106606874231705 1101 78-79 0.4402374608114705 1101 80-81 0.49070220468529513 1101 82-83 0.4373267792161464 1101 84-85 0.48683383058613217 1101 86-87 0.6955428201490719 1101 88-89 0.7791480885843853 1101 90-91 0.6973552127935925 1101 92-93 0.8154278137935549 1101 94-95 0.7496271407609001 1101 96-97 0.7069067084529976 1101 98-99 0.5923004709472757 1101 100-101 1.076342829522389 1102 1 0.11019712111017554 1102 2 0.0800765307846305 1102 3 0.03847921993637726 1102 4 0.07265485667948468 1102 5 0.09020716105106175 1102 6 0.01269019758120038 1102 7 0.04460814180725947 1102 8 0.028807409749425972 1102 9 0.11475500298409713 1102 10-11 0.1503955588384116 1102 12-13 0.16282682022290373 1102 14-15 0.22483746556864048 1102 16-17 0.22698545883302046 1102 18-19 0.15265853195101897 1102 20-21 0.1989687406288354 1102 22-23 0.14852020899291318 1102 24-25 0.09047151359515482 1102 26-27 0.10980650557489469 1102 28-29 0.20652382682244763 1102 30-31 0.15644015842621428 1102 32-33 0.09150086241207589 1102 34-35 0.19047310888004176 1102 36-37 0.22427360327311874 1102 38-39 0.2899594564368897 1102 40-41 0.16670310176745318 1102 42-43 0.046543986811535376 1102 44-45 0.1345220674513996 1102 46-47 0.20874130088666476 1102 48-49 0.0724110786777743 1102 50-51 0.15771855487577824 1102 52-53 0.08911584051183752 1102 54-55 0.11786201250635742 1102 56-57 -0.03449058214599887 1102 58-59 -0.07427753014747651 1102 60-61 -0.2592572110521232 1102 62-63 -0.08831105445770504 1102 64-65 -0.12107218378469753 1102 66-67 -0.06835038350708089 1102 68-69 0.012446421484561654 1102 70-71 0.008210518098472619 1102 72-73 -0.07926993949184435 1102 74-75 -0.003346313206670004 1102 76-77 -0.23101267089406718 1102 78-79 -0.1878743959218987 1102 80-81 -0.04536861157016148 1102 82-83 0.06187203893379234 1102 84-85 0.06508298004097668 1102 86-87 0.24381862448350589 1102 88-89 0.4098189477019609 1102 90-91 0.5319014101200672 1102 92-93 0.4570991685043708 1102 94-95 0.430752740160834 1102 96-97 0.13421088215172716 1102 98-99 0.20308766053178218 1102 100-101 0.6645202519017737 1103 1 -0.019003287726995666 1103 2 0.03585201103727087 1103 3 0.021265471476070275 1103 4 0.03986663957646641 1103 5 0.08575712567092353 1103 6 0.016647531753172018 1103 7 0.03993468739522399 1103 8 0.012294712210312753 1103 9 0.033201814840374766 1103 10-11 0.05247315927869778 1103 12-13 0.04601699502700285 1103 14-15 0.03760248902436558 1103 16-17 0.006694948572778969 1103 18-19 0.010681961465913048 1103 20-21 0.15347839809667363 1103 22-23 0.011731415324653938 1103 24-25 0.04647893032079509 1103 26-27 0.12533936979377813 1103 28-29 0.2178605409247112 1103 30-31 0.19108747084595024 1103 32-33 0.15794443764072952 1103 34-35 0.07697263714484137 1103 36-37 -0.022785586390639878 1103 38-39 0.093614749043752 1103 40-41 0.23539331588281698 1103 42-43 0.09961452432758477 1103 44-45 0.1928311588369951 1103 46-47 0.1586279534012789 1103 48-49 0.046471539405821716 1103 50-51 0.025547852252401526 1103 52-53 0.14607098635664073 1103 54-55 0.08911499361074249 1103 56-57 -0.01943764297362094 1103 58-59 -0.0791907832858314 1103 60-61 -0.025867531569979008 1103 62-63 0.020200817544818506 1103 64-65 0.005494042595785231 1103 66-67 0.1282164325423949 1103 68-69 0.15354995425705908 1103 70-71 0.003835501718775447 1103 72-73 0.07206107447675691 1103 74-75 0.011090749455490823 1103 76-77 -0.09140263865882758 1103 78-79 0.15889389036895096 1103 80-81 0.17797278057282284 1103 82-83 0.16227956573097302 1103 84-85 0.06405269726193197 1103 86-87 0.14873679153018315 1103 88-89 0.4125795813995765 1103 90-91 0.43400390769587105 1103 92-93 0.5084462345735048 1103 94-95 0.17582736604860827 1103 96-97 0.23359795398651784 1103 98-99 0.3385365951964552 1103 100-101 0.8930923356609775 1104 1 -0.03942108054168614 1104 2 -0.04059901853748471 1104 3 0.022860550776336197 1104 4 -0.0033152227969068804 1104 5 0.033997094041559706 1104 6 -0.04585741033527313 1104 7 -0.1122135817395673 1104 8 8.699000096754617E-4 1104 9 -0.013907144436821284 1104 10-11 0.03425483887581038 1104 12-13 0.023624311262338438 1104 14-15 0.04034704948636403 1104 16-17 -0.04940148210511097 1104 18-19 -0.009309677492652213 1104 20-21 0.06987025223862275 1104 22-23 0.003242041624993419 1104 24-25 -0.005168972613347478 1104 26-27 0.023315544066406346 1104 28-29 0.009956116054176789 1104 30-31 -0.0676462526693129 1104 32-33 -0.02165364904187328 1104 34-35 -0.016327187208474925 1104 36-37 -0.025623155336816694 1104 38-39 0.11975920709919308 1104 40-41 0.13375351473301578 1104 42-43 0.11533976365429766 1104 44-45 0.14737075063668925 1104 46-47 0.12419634218322528 1104 48-49 0.038240126519447415 1104 50-51 -0.03124630409259055 1104 52-53 0.004128696986825275 1104 54-55 0.1014057245213138 1104 56-57 0.08154105257428057 1104 58-59 -0.0497294547228222 1104 60-61 -0.03849205599524197 1104 62-63 0.05029538157160118 1104 64-65 0.032058173079882124 1104 66-67 0.19528527178354693 1104 68-69 0.1941337364292437 1104 70-71 -0.1584853678295346 1104 72-73 0.0057055163286392485 1104 74-75 0.03841385509627315 1104 76-77 -0.258922909323239 1104 78-79 -0.03540634231429607 1104 80-81 0.0815968707424446 1104 82-83 0.20999421440519583 1104 84-85 0.14694950645519 1104 86-87 0.10209546724492213 1104 88-89 0.2669153323053557 1104 90-91 0.354976834773332 1104 92-93 0.2608080667434223 1104 94-95 0.35379504965124653 1104 96-97 0.40243006557077265 1104 98-99 0.4357392111005858 1104 100-101 0.9231262983409181 1105 1 -0.04726328370851007 1105 2 -0.01848795279636306 1105 3 0.020682791146555246 1105 4 -0.026177161910375446 1105 5 0.05570890385055094 1105 6 0.013050987650302659 1105 7 0.014125967282708984 1105 8 -0.008902796328968066 1105 9 -0.020515283813438145 1105 10-11 -0.008954407528619868 1105 12-13 -0.053611295107614865 1105 14-15 6.675884818747591E-4 1105 16-17 0.03822498674839636 1105 18-19 -0.0030009800651313867 1105 20-21 0.056100459579489836 1105 22-23 0.031462176827567134 1105 24-25 -0.014888359211049362 1105 26-27 -0.13079384298499974 1105 28-29 -0.03893572188420791 1105 30-31 -0.00778735334532854 1105 32-33 -0.12040597425397692 1105 34-35 0.04742672595336472 1105 36-37 0.10549952512232608 1105 38-39 0.10113936226456843 1105 40-41 0.1463788493366991 1105 42-43 0.1027087578015653 1105 44-45 0.02530051788863119 1105 46-47 0.1344919276829728 1105 48-49 7.713183891482345E-4 1105 50-51 -0.0479050310106075 1105 52-53 0.11470671068856575 1105 54-55 0.07976196970075478 1105 56-57 0.00947535682545464 1105 58-59 -0.09847951143531475 1105 60-61 -0.05707106783680871 1105 62-63 -0.12400251779776283 1105 64-65 -0.24401369298789888 1105 66-67 -0.099355397877531 1105 68-69 -0.20522541242970505 1105 70-71 -0.07573050189785846 1105 72-73 -0.08537814593714899 1105 74-75 -0.06574770744625624 1105 76-77 -0.25173516829515563 1105 78-79 -0.05018788580143152 1105 80-81 -0.04095428392385969 1105 82-83 0.006960096170995911 1105 84-85 -0.17108393122912702 1105 86-87 -0.1508513137541243 1105 88-89 -0.12455624339447624 1105 90-91 0.09078510118592575 1105 92-93 4.444262552425471E-4 1105 94-95 -0.0688933492417263 1105 96-97 -0.05741068575630237 1105 98-99 0.06675640632765933 1105 100-101 0.4655506438787782 1106 1 -0.0916038612438328 1106 2 -0.13166169006310469 1106 3 -0.1787710984190909 1106 4 -0.12547745791635379 1106 5 -0.06654803875488824 1106 6 -0.04423175985012762 1106 7 -0.04406598347319601 1106 8 -0.02719131682870568 1106 9 -0.12216361929472441 1106 10-11 0.0020980025956163217 1106 12-13 0.012774518967674453 1106 14-15 -0.052750309581441 1106 16-17 -0.06640032735338508 1106 18-19 -0.17112413738377086 1106 20-21 -0.09610281355222838 1106 22-23 -0.04034796152433984 1106 24-25 -0.12888449877522135 1106 26-27 -0.05491288682095785 1106 28-29 -0.006630317862530433 1106 30-31 -0.08893080149130839 1106 32-33 -0.024755732094014604 1106 34-35 -0.09167548732394692 1106 36-37 -0.16473024731205044 1106 38-39 -0.03632760923529332 1106 40-41 -0.0507814352527447 1106 42-43 0.027919434025164946 1106 44-45 -0.00615011737668425 1106 46-47 -0.03971391711102257 1106 48-49 -0.1421332182114341 1106 50-51 -0.011968698777728548 1106 52-53 0.19399350105395285 1106 54-55 0.08948441260338313 1106 56-57 0.11182104237646939 1106 58-59 0.04980227095615675 1106 60-61 0.045542376601751755 1106 62-63 -0.030583354500151927 1106 64-65 0.0062783407438402605 1106 66-67 0.2120040522457458 1106 68-69 0.02676559557952629 1106 70-71 0.07435644618045245 1106 72-73 0.18976544344430124 1106 74-75 0.19610945054325413 1106 76-77 -0.060033045643930194 1106 78-79 0.08178708609859697 1106 80-81 0.2874909785092754 1106 82-83 0.2780990718608436 1106 84-85 0.23867691006341474 1106 86-87 0.17179373211797255 1106 88-89 0.08481060982069266 1106 90-91 0.4071348707955913 1106 92-93 0.28218709525229 1106 94-95 0.30381304578207846 1106 96-97 0.11046383830963435 1106 98-99 0.14393569885571722 1106 100-101 0.4386622081203164 1107 1 -0.13556498235821124 1107 2 -0.11017301678420921 1107 3 -0.12468652691156024 1107 4 -0.07814904993274041 1107 5 -0.17018080801909719 1107 6 -0.08662330356296621 1107 7 -0.14896247484519876 1107 8 -0.0643930359513547 1107 9 -0.12560970888081613 1107 10-11 -0.05573095736684763 1107 12-13 8.602214928998819E-5 1107 14-15 0.006633654677393963 1107 16-17 0.006711681892802801 1107 18-19 -0.010126948373837763 1107 20-21 -0.09877994694687686 1107 22-23 -0.04535589217218927 1107 24-25 -0.11799119261623758 1107 26-27 -0.05435515069707009 1107 28-29 0.01662799816299554 1107 30-31 0.05187004281322771 1107 32-33 -0.06864200861887326 1107 34-35 -0.1411632336813966 1107 36-37 -0.008058211985400021 1107 38-39 -0.09145010967787925 1107 40-41 -0.13752937306840352 1107 42-43 -0.16582028383951553 1107 44-45 -0.25543171726614844 1107 46-47 -0.43252602984424726 1107 48-49 -0.350895108785771 1107 50-51 -0.3370831606235427 1107 52-53 -0.18341915746904647 1107 54-55 -0.289387355585653 1107 56-57 -0.22951346544118678 1107 58-59 -0.19956244960724945 1107 60-61 -0.25210879867420033 1107 62-63 -0.345882919969732 1107 64-65 -0.4571591776974415 1107 66-67 -0.23594135582278852 1107 68-69 -0.23414894053514956 1107 70-71 -0.3256902237390733 1107 72-73 -0.3273437418579519 1107 74-75 -0.39041089495088244 1107 76-77 -0.6562830078618056 1107 78-79 -0.38682838255087404 1107 80-81 -0.10871738738865133 1107 82-83 -0.12377355416233371 1107 84-85 -0.13087598706989567 1107 86-87 -0.23327022402309439 1107 88-89 -0.09519836433345574 1107 90-91 -0.06035379096875815 1107 92-93 -0.025063078545478845 1107 94-95 0.00325408623702117 1107 96-97 -0.17051066703239925 1107 98-99 -0.017249681117309734 1107 100-101 0.6873455910381914 1108 1 0.04623530008163357 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-2.26497441009095 2316 94-95 -2.398023736985209 2316 96-97 -2.4835845470567435 2316 98-99 -2.8537127853991002 2316 100-101 -3.712291342301125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 19.0 5 45.0 6 105.0 7 299.0 8 719.0 9 1372.0 10 2290.0 11 3356.0 12 4796.0 13 6327.0 14 7893.0 15 10063.0 16 12748.0 17 16951.0 18 16614.0 19 13444.0 20 14043.0 21 15215.0 22 17146.0 23 19248.0 24 22764.0 25 27348.0 26 33238.0 27 41034.0 28 50007.0 29 61638.0 30 75141.0 31 91638.0 32 113590.0 33 144722.0 34 193244.0 35 274571.0 36 431884.0 37 681789.0 38 530436.0 39 40874.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.29019359687298 19.655543689667525 14.09984571347231 23.95441699998718 2 15.90792118856799 20.756467729884594 39.75174560172867 23.583865479818748 3 19.203097200049452 24.227010196786694 30.018672160138465 26.551220443025386 4 13.19867742785115 16.837900923999808 36.80767204725891 33.15574960089014 5 13.750301937337461 37.35973561879601 34.85947609546562 14.030486348400917 6 32.60196786695263 36.3273964061299 17.270841060207808 13.79979466670967 7 28.75929240643091 32.216531211197655 21.165051413950838 17.859124968420602 8 25.61858669418778 36.459794720461844 19.823968361472595 18.09765022387779 9 26.018200484844577 16.874082853595212 20.185888442745874 36.92182821881433 10-11 24.956175183698043 26.542636893339566 27.823114373867845 20.678073549094545 12-13 25.602981767263856 23.717741978832397 28.072591634012223 22.606684619891528 14-15 22.61581306031906 25.399714069407796 25.770876233980655 26.21359663629249 16-17 22.419195095533357 28.404039304537598 26.72471369557797 22.452051904351077 18-19 22.3815134192293 27.59975730895135 28.20923972930407 21.80948954251528 20-21 23.069524400797683 26.830076623718686 28.133835512983836 21.9665634624998 22-23 22.694031896537822 26.67681574831084 27.978525336085447 22.650627019065894 24-25 22.524729357822824 26.918582931535862 28.129585732023934 22.427101978617387 26-27 22.531817925273735 26.9745859739087 28.07042474964927 22.423171351168303 28-29 22.483961696203483 27.223223356393017 28.081175183698043 22.21163976370546 30-31 22.696685909943614 26.97545944667516 27.879302189325895 22.44855245405533 32-33 22.539914345916717 26.89867783099243 27.97770225597859 22.583705567112272 34-35 22.80910225379335 26.942364162382777 27.949970427266784 22.29856315655709 36-37 22.84771109976638 26.775854941097833 27.92945615678709 22.446977802348695 38-39 22.60191723648078 26.989331923959675 28.051822033200697 22.356928806358848 40-41 22.819370857506367 26.771387685078345 28.11751281310016 22.291728644315125 42-43 22.5392263890389 26.773118116830968 27.937954031101597 22.749701463028533 44-45 22.668464236677213 27.031110212251452 27.789573444430594 22.51085210664074 46-47 22.85270877185556 26.990023740041757 27.48745093632317 22.669816551779512 48-49 22.720279592242278 27.039741881774987 27.57084754338015 22.669130982602585 50-51 22.696458255224396 27.154345275769902 27.587723895489237 22.56147257351647 52-53 22.815646181714584 27.141032928579488 27.40855076086197 22.634770128843954 54-55 22.697492192497272 27.08887652588973 27.386865119679204 22.826766161933786 56-57 22.588207311370194 27.45850668408237 27.242338971935993 22.710947032611443 58-59 22.63481195188433 27.075454916375747 27.45635693657081 22.833376195169116 60-61 22.655198815542676 27.137329656149245 27.48812132691741 22.71935020139067 62-63 22.586695531582087 27.056944377254233 27.659741371432872 22.69661871973081 64-65 22.458805156794636 27.021754397988456 27.57817474975362 22.94126569546329 66-67 22.395564044030323 27.228804149721913 27.349193790871084 23.026438015376684 68-69 22.40557551690948 27.043642294777403 27.540402844198976 23.010379344114142 70-71 22.542081230279674 26.804779508597658 27.428035922575372 23.225103338547292 72-73 22.44890520267256 26.908151650997908 27.280822166319968 23.362120980009568 74-75 22.335286552819568 27.131290347722793 27.05896008363838 23.474463015819264 76-77 22.424438640189848 26.983600271114376 27.3312725052684 23.260688583427378 78-79 22.438128711630593 26.820981360420852 27.362568252680973 23.37832167526758 80-81 22.322167663769424 26.909848203871235 27.303851611758823 23.46413252060052 82-83 22.447158257139634 26.860748955864093 27.257725530668303 23.434367256327974 84-85 22.394027596364204 26.796800670827086 27.141385677196716 23.667786055611995 86-87 22.237324790812135 26.665945556399095 27.476824519722896 23.61990513306587 88-89 22.404606489643665 26.55566034393458 27.317627448631672 23.722105717790086 90-91 22.273885764694022 26.60046025272439 27.357379465123906 23.768274517457684 92-93 22.158576293142833 26.55306817387752 27.34707171614554 23.941283816834105 94-95 22.22082802530652 26.46548573148641 27.30341487537559 24.010271367831475 96-97 22.221171438419628 26.239915555056854 27.313887524549706 24.225025481973812 98-99 22.000192164008624 26.28328267191288 27.263033557479883 24.453491606598615 100-101 22.265005654904833 25.754517834745084 27.71896105632581 24.26151545402427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 255.0 2 157.0 3 179.5 4 173.5 5 182.5 6 295.0 7 544.5 8 884.0 9 1084.5 10 989.0 11 906.0 12 995.5 13 1121.0 14 1294.5 15 1442.0 16 1576.0 17 1752.5 18 1943.0 19 2216.5 20 2663.5 21 3216.5 22 4065.0 23 5476.5 24 7037.0 25 9172.5 26 12803.0 27 17339.0 28 21299.0 29 25787.5 30 31026.5 31 37456.0 32 44698.5 33 51211.5 34 58137.0 35 65063.5 36 73206.0 37 80819.0 38 85587.0 39 89434.5 40 93261.5 41 96429.5 42 97846.0 43 99804.5 44 103506.0 45 106685.0 46 109331.0 47 112304.0 48 117487.0 49 117887.0 50 111934.0 51 109472.5 52 109855.5 53 109565.0 54 107331.5 55 99489.0 56 89965.5 57 81865.0 58 74273.5 59 65719.0 60 54262.0 61 43657.5 62 34246.5 63 25228.5 64 17406.5 65 11908.5 66 8727.0 67 6820.0 68 5141.5 69 3959.0 70 2974.0 71 1776.5 72 1132.5 73 752.5 74 423.5 75 217.5 76 123.0 77 73.0 78 49.5 79 18.5 80 7.5 81 10.0 82 7.5 83 5.0 84 2.5 85 2.5 86 2.5 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4034772278930762 2 0.0 3 0.0 4 0.0 5 4.3673638323147294E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.238654260665775E-4 16-17 0.0024188476609743118 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0021668843629561543 36-37 0.006903794365697515 38-39 0.012127833411273981 40-41 0.0027044060653948904 42-43 8.230801068593145E-4 44-45 4.3673638323147294E-4 46-47 2.519632980181575E-4 48-49 1.1758287240847349E-4 50-51 4.0314127682905196E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.847730852133155E-4 60-61 0.0023852525545718906 62-63 0.0 64-65 2.519632980181575E-4 66-67 0.0013102091496944189 68-69 0.0013102091496944189 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12460424964657948 78-79 3.3595106402421E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14632348593574465 88-89 0.4360308859970221 90-91 0.335715898279393 92-93 0.0 94-95 0.0 96-97 3.3595106402420995E-4 98-99 0.0 100-101 1.5117797881089448E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2976624.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.79377313987938 #Duplication Level Percentage of deduplicated Percentage of total 1 70.12184045685399 34.215091754025494 2 15.291367970605915 14.922470795123255 3 5.843021587575653 8.553092093867528 4 2.846459760882577 5.55558047297199 5 1.56637712755595 3.821472510673046 6 1.0336568153962575 3.0261609692961096 7 0.6576081072745824 2.2461026560911006 8 0.4705858847638943 1.83693287231991 9 0.3331232309707542 1.4628905423649559 >10 1.6593348996723627 14.38066463738252 >50 0.11628113509619425 3.936779061543506 >100 0.05841474954237536 5.059040555070797 >500 0.001446205357165039 0.461634848432902 >1k 4.820684523169515E-4 0.5220862308367479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4407 0.14805363391546936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3832 0.12873644773407728 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.7190212804842E-5 2 0.0 0.0 0.0 3.3595106402421E-5 6.7190212804842E-5 3 0.0 0.0 0.0 3.3595106402421E-5 6.7190212804842E-5 4 0.0 0.0 0.0 3.3595106402421E-5 6.7190212804842E-5 5 0.0 0.0 0.0 3.3595106402421E-5 6.7190212804842E-5 6 0.0 0.0 0.0 3.3595106402421E-5 1.0078531920726299E-4 7 0.0 0.0 0.0 6.7190212804842E-5 1.34380425609684E-4 8 0.0 0.0 0.0 1.34380425609684E-4 2.0157063841452598E-4 9 0.0 0.0 0.0 2.3516574481694699E-4 2.0157063841452598E-4 10-11 0.0 0.0 0.0 2.68760851219368E-4 2.0157063841452598E-4 12-13 0.0 0.0 0.0 4.36736383231473E-4 5.207241492375255E-4 14-15 0.0 0.0 0.0 6.047119152435779E-4 7.894850004568935E-4 16-17 0.0 0.0 0.0 7.054972344508409E-4 9.74258085670209E-4 18-19 0.0 0.0 0.0 0.0010918409580786825 0.00100785319207263 20-21 0.0 0.0 0.0 0.0012934115964932085 0.00100785319207263 22-23 0.0 0.0 0.0 0.0015117797881089448 0.0011254360644811035 24-25 0.0 0.0 0.0 0.00167975532012105 0.0012934115964932085 26-27 0.0 0.0 0.0 0.0021500868097549435 0.0013941969157004715 28-29 0.0 0.0 0.0 0.0043337687259123086 0.0014109944689016819 30-31 0.0 0.0 0.0 0.011909465219658244 0.0014277920221028924 32-33 0.0 0.0 0.0 0.027413606824375533 0.001444589575304103 34-35 0.0 0.0 0.0 0.05224039045576465 0.0015117797881089448 36-37 0.0 0.0 0.0 0.09490617558683932 0.001545374894511366 38-39 0.0 0.0 0.0 0.15819935604900048 0.001646160213718629 40-41 0.0 0.0 0.0 0.227186907046372 0.0017637430861271023 42-43 0.0 0.0 0.0 0.30114653379130185 0.0017805406393283129 44-45 0.0 0.0 0.0 0.39407059810039835 0.0018309332989319444 46-47 0.0 0.0 0.0 0.5053879831648203 0.0019485161713404177 48-49 0.0 0.0 0.0 0.6256584640854874 0.0020660990437488917 50-51 0.0 0.0 0.0 0.7555035503308446 0.002116491703352523 52-53 0.0 0.0 0.0 0.8873811405135482 0.0022172770225597858 54-55 0.0 0.0 0.0 1.0399197211337408 0.0023180623417670488 56-57 0.0 0.0 0.0 1.2171675025129138 0.002334859894968259 58-59 0.0 0.0 0.0 1.4178982632673796 0.00235165744816947 60-61 0.0 0.0 0.0 1.6355945527550675 0.0024188476609743118 62-63 0.0 0.0 0.0 1.8588844274587588 0.002553228086583996 64-65 0.0 0.0 0.0 2.1009203715350004 0.002586823192986417 66-67 0.0 0.0 0.0 2.376097888077231 0.0026204182993888377 68-69 0.0 0.0 0.0 2.688263616768527 0.002654013405791259 70-71 0.0 0.0 0.0 3.0374343551620897 0.0028051913846021534 72-73 0.0 0.0 0.0 3.396885196114793 0.0033595106402421 74-75 0.0 0.0 0.0 3.772680056332274 0.0034267008530469416 76-77 0.0 0.0 0.0 4.190552787318788 0.003443498406248152 78-79 0.0 0.0 0.0 4.662564032272803 0.003493891065851784 80-81 0.0 0.0 0.0 5.167128935330764 0.0036114739382602573 82-83 0.0 0.0 0.0 5.6832169598847555 0.003930627449083257 84-85 0.0 0.0 0.0 6.214993899128677 0.003947425002284467 86-87 0.0 0.0 0.0 6.803025844043454 0.00403141276829052 88-89 0.0 0.0 0.0 7.43117034600272 0.004132198087497783 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 85 6.2856247E-4 28.034653 1 GGTATCA 7515 0.0 27.079645 1 GTATCAA 10140 0.0 27.025513 1 TACATGG 8545 0.0 24.101427 2 GTACATG 8945 0.0 23.656294 1 ATCAACG 11505 0.0 23.386303 3 TCAACGC 11560 0.0 23.233986 4 TATCAAC 11650 0.0 23.176693 2 CAACGCA 11660 0.0 22.994028 5 AACGCAG 12100 0.0 22.196724 6 ACATGGG 8815 0.0 22.125069 3 ACGCAGA 13365 0.0 19.776257 7 CGCAGAG 13445 0.0 19.552702 8 GAGTACT 6245 0.0 18.542103 12-13 CATGGGG 5015 0.0 17.978266 4 GTACTTT 6615 0.0 16.895174 14-15 CATGGGA 4290 0.0 16.81324 4 AGTACTT 6840 0.0 16.616938 12-13 GCAGAGT 14815 0.0 16.495974 9 TAAGGTG 1120 0.0 16.100178 5 >>END_MODULE