##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938957_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 475081 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.733811707898234 33.0 31.0 34.0 30.0 34.0 2 31.77961652854987 33.0 31.0 34.0 30.0 34.0 3 31.800739242360777 33.0 31.0 34.0 30.0 34.0 4 35.32531084173015 37.0 35.0 37.0 33.0 37.0 5 35.37583906744324 37.0 35.0 37.0 33.0 37.0 6 35.514032343958185 37.0 35.0 37.0 33.0 37.0 7 35.4622159168647 37.0 35.0 37.0 33.0 37.0 8 35.46288106659706 37.0 35.0 37.0 33.0 37.0 9 37.01088445970266 39.0 37.0 39.0 33.0 39.0 10-11 36.91236336540506 39.0 37.0 39.0 33.0 39.0 12-13 36.84134389714596 39.0 37.0 39.0 33.0 39.0 14-15 37.9168920668265 40.0 38.0 41.0 33.0 41.0 16-17 37.86882026433388 40.0 38.0 41.0 33.0 41.0 18-19 37.84555160067441 40.0 38.0 41.0 33.0 41.0 20-21 37.96398824621485 40.0 38.0 41.0 33.0 41.0 22-23 37.89014399649744 40.0 38.0 41.0 33.0 41.0 24-25 37.76668083968839 40.0 37.5 41.0 32.5 41.0 26-27 37.576649034585685 40.0 37.0 41.0 32.0 41.0 28-29 37.45420254651312 40.0 37.0 41.0 32.0 41.0 30-31 37.27186837612955 40.0 36.5 41.0 31.5 41.0 32-33 36.9838406503312 40.0 36.0 41.0 31.0 41.0 34-35 36.58535491842444 39.0 35.0 41.0 30.0 41.0 36-37 36.247600935419435 39.0 35.0 41.0 30.0 41.0 38-39 35.964690231771 39.0 35.0 40.5 29.5 41.0 40-41 35.834828166144305 39.0 35.0 40.0 29.0 41.0 42-43 35.56466160507366 39.0 35.0 40.0 27.5 41.0 44-45 35.39867201592992 39.0 35.0 41.0 26.5 41.0 46-47 35.04015210037868 39.0 35.0 41.0 24.5 41.0 48-49 34.693748013496645 39.0 35.0 41.0 23.0 41.0 50-51 33.50284898785681 38.0 33.0 39.5 21.0 40.5 52-53 33.6215371694511 37.5 33.5 39.5 20.5 40.5 54-55 34.13091977999541 38.0 34.0 40.0 20.5 41.0 56-57 33.96982198825042 38.0 34.0 40.0 19.5 41.0 58-59 33.765464205051344 38.0 34.0 40.0 18.0 41.0 60-61 33.33916111147362 38.0 33.0 40.0 13.5 41.0 62-63 32.98939970236654 37.0 33.0 40.0 11.5 41.0 64-65 32.56487735775583 36.5 32.0 39.5 8.0 41.0 66-67 32.04185286298547 36.0 32.0 39.0 4.5 41.0 68-69 31.54598163260581 35.0 31.0 39.0 2.0 40.0 70-71 30.96357778989267 35.0 31.0 37.5 2.0 40.0 72-73 30.48074854603741 35.0 30.0 37.0 2.0 39.0 74-75 30.014150218594303 35.0 30.0 37.0 2.0 39.0 76-77 29.590851875785393 34.0 29.0 36.0 2.0 39.0 78-79 29.165428632170094 34.0 29.0 36.0 2.0 37.5 80-81 28.675767921680723 34.0 29.0 35.0 2.0 37.0 82-83 28.167321993512687 34.0 27.5 35.0 2.0 36.5 84-85 27.719399428728998 34.0 26.5 35.0 2.0 36.0 86-87 27.317259583102672 34.0 26.0 35.0 2.0 36.0 88-89 26.759087397727967 33.5 25.0 35.0 2.0 35.0 90-91 26.218567991563543 33.0 24.0 35.0 2.0 35.0 92-93 25.65480518059026 33.0 21.5 35.0 2.0 35.0 94-95 25.076701657191087 32.0 18.5 35.0 2.0 35.0 96-97 24.35691492608629 32.0 4.5 35.0 2.0 35.0 98-99 23.540289971604842 32.0 2.0 34.5 2.0 35.0 100-101 20.62524916803661 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21610029851992962 1101 2 0.3852377047456095 1101 3 0.3224824962160149 1101 4 0.3245317192499684 1101 5 0.07036346882517108 1101 6 0.17801377753949765 1101 7 0.44727471988577605 1101 8 0.22864587833577588 1101 9 0.26229357280750776 1101 10-11 0.32765025823839977 1101 12-13 0.27963764764893995 1101 14-15 0.16276958274410447 1101 16-17 0.16636107943216416 1101 18-19 0.5802241602818938 1101 20-21 0.16376045150877871 1101 22-23 0.3807302514650601 1101 24-25 0.5598941663276307 1101 26-27 0.45960341614184586 1101 28-29 0.2243609068498813 1101 30-31 0.1817834713615909 1101 32-33 0.1760939214592696 1101 34-35 0.20976743678230747 1101 36-37 0.0724942624572833 1101 38-39 0.4976837369699112 1101 40-41 0.2775382107066875 1101 42-43 0.35916975359283043 1101 44-45 0.34138022472571805 1101 46-47 0.40061947165538925 1101 48-49 0.5076756002021483 1101 50-51 0.5247848122826824 1101 52-53 0.7675598564279298 1101 54-55 0.6664135688795554 1101 56-57 0.7726260473923006 1101 58-59 0.9442205675148116 1101 60-61 0.9162838369722479 1101 62-63 0.6778847331140909 1101 64-65 0.5435526616202893 1101 66-67 0.5961643583299434 1101 68-69 0.7825805269194959 1101 70-71 0.5245298112609014 1101 72-73 0.36194056083202497 1101 74-75 0.32440860921582626 1101 76-77 0.10149577997227155 1101 78-79 0.6686816403670655 1101 80-81 0.395219493296711 1101 82-83 -0.05684162537035675 1101 84-85 0.29515904078484567 1101 86-87 0.21443064260966693 1101 88-89 0.09385529311649421 1101 90-91 -0.07792232226662321 1101 92-93 0.08089630492116484 1101 94-95 -0.15392003302089918 1101 96-97 0.0031946825856898897 1101 98-99 0.025047570842950506 1101 100-101 0.7819461475004523 1102 1 0.2537554142879088 1102 2 0.31708666250084505 1102 3 0.4148415878314431 1102 4 0.2997020968555759 1102 5 0.14935319067124198 1102 6 0.33713092185543303 1102 7 0.38614412295368794 1102 8 0.5226499185604325 1102 9 0.47613689200746023 1102 10-11 0.4675485866509561 1102 12-13 0.44128825701249497 1102 14-15 0.7299743910554213 1102 16-17 0.7944300962368374 1102 18-19 0.5983524485141842 1102 20-21 0.5580736133175606 1102 22-23 0.43443527066722964 1102 24-25 0.46928879860058714 1102 26-27 0.6743707702386814 1102 28-29 0.49581905056644615 1102 30-31 0.267957356414982 1102 32-33 0.24635387217741567 1102 34-35 0.22001506703514906 1102 36-37 0.2986219735146207 1102 38-39 0.5897764401466006 1102 40-41 0.3497849751971174 1102 42-43 0.1743072766243401 1102 44-45 0.459062867299032 1102 46-47 0.7327337078975376 1102 48-49 0.5387403769908303 1102 50-51 0.533386938950386 1102 52-53 0.5181564555355251 1102 54-55 0.33019806063261115 1102 56-57 0.5074585556614366 1102 58-59 0.30811827435433514 1102 60-61 0.40424085963746137 1102 62-63 0.44229468272227024 1102 64-65 0.5540999788667165 1102 66-67 0.15244446577327864 1102 68-69 0.2292390169410261 1102 70-71 0.48447997442382373 1102 72-73 0.636642538766182 1102 74-75 0.7021252163150749 1102 76-77 0.7237181255224776 1102 78-79 0.33517163970109465 1102 80-81 0.10733683740397382 1102 82-83 0.2951046751602 1102 84-85 0.20357136568994605 1102 86-87 0.4054256034283661 1102 88-89 0.3178796454596018 1102 90-91 0.2832726518714992 1102 92-93 0.12987292933566508 1102 94-95 0.36518223794054094 1102 96-97 0.7707124973125303 1102 98-99 1.1481107049029333 1102 100-101 1.0123915711690685 1103 1 0.06736089404264334 1103 2 0.2843683285230476 1103 3 0.2149189230797397 1103 4 0.18895249734168829 1103 5 0.18447996523899235 1103 6 0.1491178853421502 1103 7 0.29194979052259384 1103 8 0.18361219000715323 1103 9 0.11675999315091445 1103 10-11 0.3938315231808076 1103 12-13 0.37544834097647595 1103 14-15 0.06805647689754579 1103 16-17 -0.1234509179597012 1103 18-19 0.03395704443750702 1103 20-21 0.10727816504931553 1103 22-23 0.06653768463176135 1103 24-25 0.03623626587120299 1103 26-27 -0.004627185901185271 1103 28-29 -0.08579449414989426 1103 30-31 -0.186674472563638 1103 32-33 0.2986325000291501 1103 34-35 -0.16386329566715574 1103 36-37 -0.10803714375875728 1103 38-39 -0.045187378005088874 1103 40-41 -0.14516337590491446 1103 42-43 -0.1150315070026906 1103 44-45 -6.758500406292001E-4 1103 46-47 -0.19460982632330825 1103 48-49 -0.1933730848663231 1103 50-51 -0.48841883909745576 1103 52-53 -0.20420171714302882 1103 54-55 -0.25730953479357055 1103 56-57 -0.29954304628298445 1103 58-59 -0.49528171459777326 1103 60-61 -0.35244035342114444 1103 62-63 -0.21833891391916893 1103 64-65 -0.7698629002140933 1103 66-67 -0.8383498777056992 1103 68-69 -0.7319000207875277 1103 70-71 -0.8534695367069283 1103 72-73 -0.8584245771814523 1103 74-75 -0.9679626144312081 1103 76-77 -1.4134248894474162 1103 78-79 -1.5958315089483008 1103 80-81 -1.689033929872167 1103 82-83 -1.5925023512995047 1103 84-85 -1.5644366992716634 1103 86-87 -1.3893294095529107 1103 88-89 -1.3983800904957633 1103 90-91 -1.2181635741683863 1103 92-93 -1.0597144318751823 1103 94-95 -1.1326855187848643 1103 96-97 -1.7869215964840777 1103 98-99 -2.0626996580203425 1103 100-101 -1.2107565258314317 1104 1 0.22111002926695278 1104 2 0.13882524938941998 1104 3 0.14825935200848406 1104 4 0.2013147984432777 1104 5 0.31479234882688445 1104 6 0.24342899172286536 1104 7 0.41740346515406657 1104 8 0.21459510736120535 1104 9 0.4714297818795643 1104 10-11 0.30833846799480824 1104 12-13 0.27653396707408007 1104 14-15 0.5448751666958529 1104 16-17 0.22577477519127598 1104 18-19 0.31929310957507795 1104 20-21 0.17109861100747992 1104 22-23 0.31731465232906686 1104 24-25 0.3126904194553717 1104 26-27 0.21924594485290072 1104 28-29 0.5066247491048514 1104 30-31 0.5554307905942508 1104 32-33 0.42806680368514805 1104 34-35 0.5153155665541149 1104 36-37 0.541994786420581 1104 38-39 0.3027052301583879 1104 40-41 0.4190083020260076 1104 42-43 0.33038385141357907 1104 44-45 0.523008360485683 1104 46-47 0.5659085069213248 1104 48-49 0.7341533905740505 1104 50-51 0.4039631522813991 1104 52-53 0.3262357263139464 1104 54-55 0.330820421036357 1104 56-57 0.4513509987385689 1104 58-59 0.38200794534581206 1104 60-61 0.3419559001044803 1104 62-63 0.5573063417883546 1104 64-65 0.49959588324607296 1104 66-67 0.6887615054032352 1104 68-69 0.3151437767751375 1104 70-71 0.5607340714033349 1104 72-73 0.4685617460583984 1104 74-75 0.3307282482158058 1104 76-77 0.17501638563107846 1104 78-79 0.3109403499203083 1104 80-81 0.026728935329259684 1104 82-83 0.010656075606991777 1104 84-85 -0.12604864115873937 1104 86-87 -0.32452977142827777 1104 88-89 -0.16238147413686832 1104 90-91 -0.05244594338768849 1104 92-93 -0.03256313604207506 1104 94-95 0.3039490403125775 1104 96-97 0.5767072683980459 1104 98-99 0.9208350128327254 1104 100-101 0.7375990699325001 1105 1 0.11380995025288243 1105 2 0.27903930568438184 1105 3 0.26357884590798264 1105 4 0.25347434529246016 1105 5 0.29823530990475433 1105 6 0.3532893317912027 1105 7 0.2250606467119809 1105 8 0.48362261862327927 1105 9 0.35149771345543 1105 10-11 0.13285540471172652 1105 12-13 0.2724344709170339 1105 14-15 0.3207522741652511 1105 16-17 0.5350327612560619 1105 18-19 0.4836911147263834 1105 20-21 0.36872357124391186 1105 22-23 0.2183418352209756 1105 24-25 0.46559803456450055 1105 26-27 0.4168401203872705 1105 28-29 0.40754162014773954 1105 30-31 0.3691551238489197 1105 32-33 0.33466858304092995 1105 34-35 0.24911511709746748 1105 36-37 0.22348856277998408 1105 38-39 0.39250164066212534 1105 40-41 0.3866074031127127 1105 42-43 0.28258033990523046 1105 44-45 0.4450640602732676 1105 46-47 0.5574863255173383 1105 48-49 0.3528361131730975 1105 50-51 0.511111873177029 1105 52-53 0.3197632427067916 1105 54-55 0.45944403700745085 1105 56-57 0.30447718523229383 1105 58-59 0.2785841733988832 1105 60-61 0.4298205705542415 1105 62-63 0.45168402903630067 1105 64-65 0.5706418448062394 1105 66-67 0.6711193057336544 1105 68-69 0.6333883304482626 1105 70-71 0.5483919712709628 1105 72-73 0.5114627937644265 1105 74-75 0.479169516135066 1105 76-77 0.48884333455925955 1105 78-79 0.541628080164223 1105 80-81 0.39956759270578956 1105 82-83 0.454353420939519 1105 84-85 0.8273664084559407 1105 86-87 0.5214902796743281 1105 88-89 0.7127993859877044 1105 90-91 1.1345582946396249 1105 92-93 0.7509331851860317 1105 94-95 0.7310072541841599 1105 96-97 0.7089121713749265 1105 98-99 0.3634292396749359 1105 100-101 0.6860731748672855 1106 1 0.2898755886503608 1106 2 0.34777236634569064 1106 3 0.36994064554332695 1106 4 0.09258142655200885 1106 5 0.2955200879070219 1106 6 0.3664958562084877 1106 7 0.33043688482364075 1106 8 0.46728200856069435 1106 9 0.6758706499359874 1106 10-11 0.6291496933826934 1106 12-13 0.581683965816751 1106 14-15 0.6615397378226717 1106 16-17 0.6452431786413726 1106 18-19 0.7349920559376457 1106 20-21 0.6266622693171371 1106 22-23 0.8221855251633201 1106 24-25 0.7454235901748092 1106 26-27 0.5472767241422645 1106 28-29 0.4849057358526565 1106 30-31 0.27706179117262053 1106 32-33 0.36914106123538204 1106 34-35 0.35168743267426805 1106 36-37 0.4516485194152793 1106 38-39 0.2644599514096839 1106 40-41 0.3379058804201591 1106 42-43 0.17183279148951414 1106 44-45 0.23393953457475192 1106 46-47 -0.17258069266626563 1106 48-49 -0.2639455356713256 1106 50-51 -0.5585057289517792 1106 52-53 -0.3359037360543553 1106 54-55 -0.3214163582027183 1106 56-57 -0.32999282905835514 1106 58-59 -0.28008876800948457 1106 60-61 -0.22095555914565068 1106 62-63 -0.34536421420028063 1106 64-65 -0.4820019239810236 1106 66-67 -0.3919788069160255 1106 68-69 -0.21919876855139364 1106 70-71 0.04972494323173393 1106 72-73 -0.016564112065172054 1106 74-75 0.10855156905972052 1106 76-77 -0.30095363976686684 1106 78-79 -0.19724985804106865 1106 80-81 -0.0819038506490628 1106 82-83 0.1793990031599897 1106 84-85 0.20789910445948934 1106 86-87 0.015360086997052491 1106 88-89 0.11050056373372641 1106 90-91 -0.09792334927699642 1106 92-93 0.03310437722269555 1106 94-95 0.13567635190258542 1106 96-97 0.25786501225602265 1106 98-99 0.24895380479591367 1106 100-101 0.2959263262002594 1107 1 0.24997630164186546 1107 2 0.2796922665739423 1107 3 0.20487958240945758 1107 4 0.01761142866980947 1107 5 0.2627977780969175 1107 6 0.043555413353701056 1107 7 0.10487743356826229 1107 8 0.26160631380577115 1107 9 0.06465103443371589 1107 10-11 0.03735027224708887 1107 12-13 0.40195598501811247 1107 14-15 0.3652976501458909 1107 16-17 0.42155680077940616 1107 18-19 0.2516379368233501 1107 20-21 0.4076491625095997 1107 22-23 0.5482791317708475 1107 24-25 0.6367217994601688 1107 26-27 0.6848205507653233 1107 28-29 0.5987655377210217 1107 30-31 0.20583088075542122 1107 32-33 0.5668561940682864 1107 34-35 0.29993083809112164 1107 36-37 0.43061879967036276 1107 38-39 0.7081242100632323 1107 40-41 0.5995878815025861 1107 42-43 0.4551492608873602 1107 44-45 -0.1626672847301407 1107 46-47 0.20897565248750283 1107 48-49 0.28649096572877397 1107 50-51 0.6073059754038184 1107 52-53 0.39021340162594953 1107 54-55 0.605025508281976 1107 56-57 0.3981338622375077 1107 58-59 0.3939493993905714 1107 60-61 0.4007542086026632 1107 62-63 -0.32069188438877205 1107 64-65 -0.0019280364124938387 1107 66-67 0.041235302315214994 1107 68-69 0.13660752365747442 1107 70-71 0.04107256776994106 1107 72-73 0.2575258236950013 1107 74-75 0.21941351225570926 1107 76-77 -0.21779718166394346 1107 78-79 -0.02712726115187536 1107 80-81 0.25503758051078407 1107 82-83 0.12942135767913143 1107 84-85 0.13892717702931634 1107 86-87 0.4119080786199909 1107 88-89 0.030913769371771593 1107 90-91 0.25628988930455066 1107 92-93 0.3356692641558894 1107 94-95 0.36974463907579747 1107 96-97 0.3680233763758416 1107 98-99 0.318386941252605 1107 100-101 0.45900842611319703 1108 1 0.3045196402509376 1108 2 0.3200609473299245 1108 3 0.46153166322129735 1108 4 0.4097816176652458 1108 5 0.3075841108406152 1108 6 0.4545273898921991 1108 7 0.5458931676712311 1108 8 0.5529246268120076 1108 9 0.7877913379436379 1108 10-11 0.5522743947682685 1108 12-13 0.6104584521999286 1108 14-15 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-0.5942351779342268 2316 84-85 -1.0006071317554515 2316 86-87 -0.8892736451677656 2316 88-89 -0.8939457892028564 2316 90-91 -1.253770166757917 2316 92-93 -1.2605364402191874 2316 94-95 -1.236812083285674 2316 96-97 -1.7137236122633333 2316 98-99 -2.350819778636641 2316 100-101 -2.1597858124603704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2068.0 3 426.0 4 408.0 5 407.0 6 449.0 7 662.0 8 829.0 9 945.0 10 1367.0 11 1815.0 12 2525.0 13 3561.0 14 3425.0 15 3758.0 16 4718.0 17 6356.0 18 5292.0 19 3764.0 20 3898.0 21 4060.0 22 4356.0 23 4845.0 24 5373.0 25 6041.0 26 6813.0 27 7568.0 28 9101.0 29 10687.0 30 12657.0 31 14809.0 32 18552.0 33 23440.0 34 31000.0 35 44832.0 36 61968.0 37 84488.0 38 67398.0 39 10420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.88539895801876 19.582869608739635 15.417267660779563 19.114463772462045 2 15.01627463579577 23.503102187858808 37.49170467592934 23.98891850041608 3 22.888994707211 25.607027273033744 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20.08222277463418 26.753685594266525 31.086128514428484 22.07796311667081 24-25 22.663742394363823 27.04172355092616 29.851068581348706 20.44346547336131 26-27 21.541626463636096 25.080686955679056 33.524587401037344 19.853099179647504 28-29 19.486961257179814 29.451958839769404 31.251270588805337 19.809809314245445 30-31 20.29961387033374 27.016121387538945 30.440309999136318 22.243954742990997 32-33 20.476712553830794 27.58327251204051 31.22602034959342 20.71399458453528 34-35 22.413711187059132 26.50540205819843 30.267363079759146 20.81352367498329 36-37 21.336583921224364 25.816366463240502 32.675494727490026 20.171554888045097 38-39 21.008910737189822 27.219462987185327 31.58474082517635 20.186885450448496 40-41 20.489258162496128 28.01314330238159 31.043017039141287 20.454581495980996 42-43 19.76027639915439 28.450098298520814 28.959621992218608 22.830003310106186 44-45 21.001920668765017 26.56265864554777 29.531445662842255 22.903975022844957 46-47 22.54443889810146 28.317371146231686 29.261671291752684 19.876518663914176 48-49 21.343874353967585 27.38817795059373 29.85468369533714 21.413264000101545 50-51 19.071865629412603 28.422911962091923 31.74917631379932 20.75604609469615 52-53 20.86255222380877 29.547093976413347 28.054577185467238 21.535776614310645 54-55 19.458669391087675 27.252247964616373 29.832304272137506 23.45677837215845 56-57 21.74015564860635 27.204880937275306 28.421520111660957 22.633443302457387 58-59 23.427950391327467 26.607730554413177 28.41471936804023 21.54959968621912 60-61 20.829537696747835 27.478541883748896 31.094770792867095 20.597149626636174 62-63 20.168677756744465 27.61482249177762 30.53331782273501 21.6831819287429 64-65 19.80748991173095 29.59292578312998 29.30521839846415 21.294365906674916 66-67 20.754703009312397 27.329024996323913 28.126444648730192 23.789827345633498 68-69 19.665073643382147 27.704956711170453 29.52950972477763 23.10045992066977 70-71 22.10788919076759 27.618409804568806 27.823024316752576 22.450676687911027 72-73 20.862635828171015 25.83570292136353 30.822733658305406 22.47892759216005 74-75 19.467153284671532 29.758383581931664 28.54860890722522 22.225854226171585 76-77 20.479793386157212 26.758163658131085 28.75079849227736 24.01124446343434 78-79 20.54648079143598 26.851808772961245 30.037304752760846 22.56440568284193 80-81 21.708450358689767 26.916748443421767 28.773539015971846 22.60126218191662 82-83 20.381668490934185 26.296631185985397 30.648117303045204 22.673583020035217 84-85 20.153811498424766 27.13804222413288 29.67068645149555 23.037459825946808 86-87 20.153072825396087 27.54010044809283 29.240780360483647 23.066046366027436 88-89 20.214718054317256 26.948268622841 28.13893745128337 24.698075871558373 90-91 20.660052042564896 25.390319236709047 29.073176923565125 24.876451797160932 92-93 21.48118294072285 26.852800952885687 29.239246450215212 22.426769656176248 94-95 20.638791266685992 26.03321435296756 29.52571478142383 23.802279598922617 96-97 18.69651406580899 26.18925214366864 30.801974311942132 24.312259478580238 98-99 20.458522074520417 26.833231425434096 28.26759392276298 24.440652577282506 100-101 20.081078194027118 24.68601501628227 29.84969968493094 25.38320710475967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2842.0 1 1742.5 2 631.0 3 592.0 4 511.5 5 540.5 6 845.5 7 1386.5 8 1868.0 9 1965.0 10 1578.0 11 1227.5 12 1200.5 13 1171.5 14 1202.5 15 1289.0 16 1422.0 17 1482.5 18 1414.0 19 1422.5 20 1536.0 21 1666.5 22 1808.5 23 2031.0 24 2277.5 25 2619.5 26 3123.5 27 3730.5 28 4303.5 29 4847.5 30 5374.5 31 6090.5 32 7094.5 33 8050.5 34 8648.5 35 9116.5 36 9785.0 37 10450.5 38 10780.5 39 10962.0 40 11077.5 41 10957.5 42 10747.5 43 11075.5 44 11794.5 45 12471.5 46 15385.5 47 25182.5 48 44249.5 49 44438.5 50 25879.5 51 16138.0 52 12445.5 53 11259.5 54 10664.0 55 9669.5 56 8514.5 57 7427.5 58 6854.0 59 6385.5 60 5292.0 61 4381.5 62 3674.0 63 2676.0 64 1812.5 65 1354.0 66 1015.5 67 790.5 68 596.5 69 429.5 70 324.5 71 236.0 72 183.5 73 128.5 74 93.5 75 76.0 76 50.5 77 33.0 78 23.0 79 17.5 80 16.0 81 13.0 82 9.5 83 6.0 84 4.0 85 1.5 86 2.0 87 4.0 88 3.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3283650577480472 2 0.08756401539947925 3 0.09956196943258096 4 0.10482422997341508 5 0.11766414569305023 6 0.054306528781407806 7 0.05725339468427489 8 0.05809535637080835 9 0.06146320311694216 10-11 0.05872682763570844 12-13 0.055885206943658026 14-15 0.054727509624674535 16-17 0.055990452154474714 18-19 0.06462055944144261 20-21 0.06725168971185966 22-23 0.08314371654517862 24-25 0.09114235256724643 26-27 0.08566960160477897 28-29 0.08409092344252875 30-31 0.07788145600434451 32-33 0.0693565939281933 34-35 0.31815627229882903 36-37 0.4938105291518709 38-39 0.4682359429234173 40-41 0.4505547475062147 42-43 0.4812863490646858 44-45 0.4902321919841038 46-47 0.4834964984918361 48-49 0.5026511268604722 50-51 0.496441659422288 52-53 0.4959154333682046 54-55 0.4883377781894035 56-57 0.4674992264477005 58-59 0.4503442570845814 60-61 0.41045632218505895 62-63 0.48118110385386914 64-65 0.5006514678549553 66-67 0.5118074602015236 68-69 0.5261208088725923 70-71 0.5230686977589085 72-73 0.5104392724609066 74-75 0.5117022149907068 76-77 0.48728532608123665 78-79 0.4670782456044338 80-81 0.4733929582534347 82-83 0.48170732990795256 84-85 0.48297027243775276 86-87 0.48402272454591955 88-89 0.48865351382185357 90-91 0.5032825981253722 92-93 0.5075976517688563 94-95 0.5165434946882742 96-97 0.5072819161364062 98-99 0.5119127054123402 100-101 0.48991645635165376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 475081.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.58162373532602 #Duplication Level Percentage of deduplicated Percentage of total 1 73.61092513096742 33.55305492129011 2 13.750345418962068 12.535261422357857 3 5.288590835663939 7.231876726839811 4 2.561202632655468 4.669750988465119 5 1.4159171964326513 3.226990244408541 6 0.8560661560763816 2.34125312512923 7 0.5588748704522993 1.7832096842060046 8 0.3867198639736103 1.410185546449725 9 0.29514003476079287 1.2107665812327755 >10 1.1710021648674256 8.68731128101388 >50 0.05237609496156169 1.586450653675842 >100 0.033835884355517196 3.216212318658098 >500 0.006025568446872926 1.8442039893297335 >1k 0.012051136893745852 12.805904044591951 >5k 4.635052651440712E-4 1.5188432213645842 >10k+ 4.635052651440712E-4 2.378725250986765 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11259 2.3699116571700407 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7189 1.5132156411222506 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4799 1.0101435334185118 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3570 0.751450805231108 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3355 0.7061953645799348 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3311 0.6969337860280668 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3158 0.6647287515181621 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3039 0.639680391343792 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3006 0.6327342074298908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2975 0.6262090043592566 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2952 0.6213677246616892 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2637 0.5550632418471798 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2623 0.5521163759443126 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2320 0.4883377781894035 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2210 0.4651838318097335 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2051 0.43171585477002866 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2007 0.42245427621816073 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1983 0.4174025060989599 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1823 0.3837240386376218 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1745 0.36730578575021944 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1714 0.3607805826795852 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1628 0.3426784064191159 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1577 0.33194339491581437 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1477 0.31089435275247795 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1333 0.28058373203727366 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1168 0.24585281246776863 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1087 0.22880308831546622 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1065 0.2241722990395322 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 979 0.20607012277906295 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 911 0.19175677410799422 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 736 0.1549209503221556 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 710 0.14944819935968814 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 709 0.14923770893805477 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 651 0.13702926448331967 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 647 0.13618730279678623 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 638 0.13429288900208597 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 589 0.12397885834205115 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 572 0.12040052117428396 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 543 0.11429629894691641 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 539 0.11345433726038298 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 505 0.1062976629248486 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 492 0.10356128744361487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.1049042163336358E-4 5 0.0 0.0 0.0 0.0 2.1049042163336358E-4 6 0.0 0.0 0.0 0.0 2.1049042163336358E-4 7 0.0 0.0 0.0 0.0 2.1049042163336358E-4 8 0.0 0.0 0.0 0.0 2.1049042163336358E-4 9 0.0 0.0 0.0 0.0 2.1049042163336358E-4 10-11 0.0 0.0 0.0 0.0 2.1049042163336358E-4 12-13 0.0 0.0 0.0 0.0 2.1049042163336358E-4 14-15 0.0 0.0 0.0 2.1049042163336358E-4 2.1049042163336358E-4 16-17 0.0 0.0 0.0 2.1049042163336358E-4 2.1049042163336358E-4 18-19 0.0 0.0 0.0 2.1049042163336358E-4 2.1049042163336358E-4 20-21 0.0 0.0 0.0 8.419616865334543E-4 2.1049042163336358E-4 22-23 0.0 0.0 0.0 0.0027363754812337266 2.1049042163336358E-4 24-25 0.0 0.0 0.0 0.006314712649000907 2.1049042163336358E-4 26-27 0.0 0.0 0.0 0.011997954033101724 2.1049042163336358E-4 28-29 0.0 0.0 0.0 0.033152241407254764 2.1049042163336358E-4 30-31 0.0 0.0 0.0 0.08545911118314561 2.1049042163336358E-4 32-33 0.0 0.0 0.0 0.1802850461289759 2.1049042163336358E-4 34-35 0.0 0.0 0.0 0.3103681266983946 2.1049042163336358E-4 36-37 0.0 0.0 0.0 0.4864433643947032 2.1049042163336358E-4 38-39 0.0 0.0 0.0 0.7418734910467899 2.1049042163336358E-4 40-41 0.0 0.0 0.0 1.002144897396444 2.1049042163336358E-4 42-43 0.0 0.0 0.0 1.2573645336268973 2.1049042163336358E-4 44-45 0.0 0.0 0.0 1.5626808902060914 2.1049042163336358E-4 46-47 0.0 0.0 0.0 1.9124107257499248 2.1049042163336358E-4 48-49 0.0 0.0 0.0 2.243722649400839 2.1049042163336358E-4 50-51 0.0 0.0 0.0 2.588716450457922 2.1049042163336358E-4 52-53 0.0 0.0 0.0 2.906030761070218 2.1049042163336358E-4 54-55 0.0 0.0 0.0 3.2684952671228693 2.1049042163336358E-4 56-57 0.0 0.0 0.0 3.7316794399270865 2.1049042163336358E-4 58-59 0.0 0.0 0.0 4.164342501594465 2.1049042163336358E-4 60-61 0.0 0.0 0.0 4.66183661312492 2.1049042163336358E-4 62-63 0.0 0.0 0.0 5.143754433454506 2.1049042163336358E-4 64-65 0.0 0.0 0.0 5.614831997069973 2.1049042163336358E-4 66-67 0.0 0.0 0.0 6.183682361534139 2.1049042163336358E-4 68-69 0.0 0.0 0.0 6.8420964004033 2.1049042163336358E-4 70-71 0.0 0.0 0.0 7.506614661499828 2.1049042163336358E-4 72-73 0.0 0.0 0.0 8.136086267394402 2.1049042163336358E-4 74-75 0.0 0.0 0.0 8.871013574527291 2.1049042163336358E-4 76-77 0.0 0.0 0.0 9.570894226458226 2.1049042163336358E-4 78-79 0.0 0.0 0.0 10.497052081645023 2.1049042163336358E-4 80-81 0.0 0.0 0.0 11.41731620502609 2.1049042163336358E-4 82-83 0.0 0.0 0.0 12.269591922219579 2.1049042163336358E-4 84-85 0.0 0.0 0.0 13.223850248694433 2.1049042163336358E-4 86-87 0.0 0.0 0.0 14.175582690109685 2.1049042163336358E-4 88-89 0.0 1.0524521081668179E-4 0.0 15.157520507029329 2.1049042163336358E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCTC 25 0.0016049978 38.0513 60-61 TTTGCGT 340 0.0 30.647064 3 GTACCCA 110 2.745297E-6 30.204157 1 ATATGAG 65 0.005923281 29.146858 3 GTAAGCG 75 3.291825E-8 28.538475 94-95 CTTTGCG 385 0.0 27.064941 2 CTAGGGC 70 0.008505118 27.06494 3 TTGCGTT 425 0.0 24.51765 4 GTAGCCC 175 1.5240767E-7 24.358446 3 GAGTACT 2565 0.0 23.346378 12-13 AACTCTA 145 2.3946475E-5 22.85076 7 GGTAAGG 130 2.9956503E-4 21.860144 3 GTACTTT 2775 0.0 21.323742 14-15 GTGTAGC 225 7.187191E-8 21.094967 1 GTTATAA 135 3.8110343E-4 21.094967 1 TAAGGTG 140 4.9408193E-4 20.298704 5 GCTTTGC 545 0.0 20.030542 1 AGGTAAG 145 6.25874E-4 19.59875 2 GTACATG 2790 0.0 19.053518 1 TACACAA 175 1.0142421E-4 18.94546 5 >>END_MODULE