##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938956_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480639 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.746701786579948 33.0 31.0 34.0 30.0 34.0 2 31.763414953842698 33.0 31.0 34.0 30.0 34.0 3 31.801139732730803 33.0 31.0 34.0 30.0 34.0 4 35.31395912524785 37.0 35.0 37.0 33.0 37.0 5 35.3737045890991 37.0 35.0 37.0 33.0 37.0 6 35.49941432135137 37.0 35.0 37.0 33.0 37.0 7 35.437282451070345 37.0 35.0 37.0 33.0 37.0 8 35.43455067108578 37.0 35.0 37.0 33.0 37.0 9 36.98299555383562 39.0 37.0 39.0 33.0 39.0 10-11 36.8858654832421 39.0 37.0 39.0 33.0 39.0 12-13 36.80107315469614 39.0 37.0 39.0 33.0 39.0 14-15 37.88675492417386 40.0 38.0 41.0 33.0 41.0 16-17 37.84596235428253 40.0 38.0 41.0 32.5 41.0 18-19 37.8501921400469 40.0 38.0 41.0 33.0 41.0 20-21 37.738947526105875 40.0 37.5 41.0 32.0 41.0 22-23 37.797116963042946 40.0 38.0 41.0 32.5 41.0 24-25 37.68042231279609 40.0 37.0 41.0 32.0 41.0 26-27 37.53452799294273 40.0 37.0 41.0 32.0 41.0 28-29 37.403736484138825 40.0 37.0 41.0 32.0 41.0 30-31 37.22247778478234 40.0 36.5 41.0 31.5 41.0 32-33 36.954207835818565 40.0 35.5 41.0 31.0 41.0 34-35 36.574277160197155 39.0 35.0 41.0 30.0 41.0 36-37 36.22939461841423 39.0 35.0 41.0 30.0 41.0 38-39 35.9580121463302 39.0 35.0 41.0 29.5 41.0 40-41 35.846777935207086 39.0 35.0 41.0 29.0 41.0 42-43 35.576317152790345 39.0 35.0 40.5 27.5 41.0 44-45 35.40095997203723 39.0 35.0 41.0 26.5 41.0 46-47 35.05485717971284 39.0 35.0 41.0 24.0 41.0 48-49 34.75438842873758 39.0 35.0 41.0 23.0 41.0 50-51 33.50440039197818 38.0 33.0 40.0 21.0 40.5 52-53 33.66796805918787 38.0 33.5 39.5 21.5 40.5 54-55 34.17787674325221 38.0 34.0 40.0 20.5 41.0 56-57 34.024466387455035 38.0 34.0 40.0 19.5 41.0 58-59 33.82574135681874 38.0 34.0 40.0 18.0 41.0 60-61 33.434856513932495 38.0 33.0 40.0 14.5 41.0 62-63 33.097789609249354 37.0 33.0 40.0 12.5 41.0 64-65 32.66259084260744 36.5 32.5 39.5 8.0 41.0 66-67 32.17783513198055 36.0 32.0 39.0 7.0 41.0 68-69 31.72316957217371 35.5 31.5 39.0 2.0 40.0 70-71 31.15496557707552 35.0 31.0 37.5 2.0 40.0 72-73 30.667764163956733 35.0 30.5 37.0 2.0 39.5 74-75 30.196641346207862 35.0 30.0 37.0 2.0 39.0 76-77 29.772434196975276 34.5 29.5 36.0 2.0 39.0 78-79 29.299495047218393 34.0 29.0 36.0 2.0 37.5 80-81 28.812491287639997 34.0 29.0 35.0 2.0 37.0 82-83 28.402817915316902 34.0 28.5 35.0 2.0 37.0 84-85 28.02663017358142 34.0 27.5 35.0 2.0 36.0 86-87 27.68751391376896 34.0 27.0 35.0 2.0 36.0 88-89 27.160567494522915 34.0 25.5 35.0 2.0 35.5 90-91 26.65288917461962 33.5 25.0 35.0 2.0 35.0 92-93 26.159288988201126 33.0 24.0 35.0 2.0 35.0 94-95 25.61074111755392 33.0 20.5 35.0 2.0 35.0 96-97 24.95347027602837 32.5 14.0 35.0 2.0 35.0 98-99 24.250100387192884 32.0 2.0 35.0 2.0 35.0 100-101 21.784831235084958 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4294607969287263 1101 2 0.2792358260185104 1101 3 0.13441833784631996 1101 4 0.019298857819698867 1101 5 -0.03362503175782905 1101 6 0.2543755517777342 1101 7 0.15353552386063285 1101 8 0.3255500477239295 1101 9 0.26288601758135854 1101 10-11 0.44002763904011744 1101 12-13 0.5246102455646806 1101 14-15 0.08928873787066749 1101 16-17 0.33100815382903903 1101 18-19 0.41768896590340887 1101 20-21 0.46130557673429706 1101 22-23 0.6031064363897514 1101 24-25 0.5374318958586599 1101 26-27 0.6367951543226624 1101 28-29 0.6981476844499426 1101 30-31 0.36684476382244213 1101 32-33 0.3565065763100961 1101 34-35 0.19367258949372967 1101 36-37 0.38796491557435075 1101 38-39 0.4058053804975259 1101 40-41 0.3200635730440453 1101 42-43 0.49195751909343954 1101 44-45 0.3337632692545327 1101 46-47 0.30877811640058894 1101 48-49 0.1629211364821188 1101 50-51 0.41869766410039233 1101 52-53 0.20307858107051402 1101 54-55 0.1417162102670062 1101 56-57 0.15940035157731103 1101 58-59 0.5712103873750962 1101 60-61 0.6370389960231293 1101 62-63 0.5927349918069638 1101 64-65 0.8270993930754287 1101 66-67 0.7738897637521589 1101 68-69 0.4471274981616844 1101 70-71 0.5366341389997551 1101 72-73 0.5257412979014298 1101 74-75 0.21634608107732234 1101 76-77 0.5256837896614464 1101 78-79 -0.0015237189259060813 1101 80-81 -0.19785504243790442 1101 82-83 -0.03416274073828163 1101 84-85 -0.14559609253460692 1101 86-87 -0.3471311693254222 1101 88-89 0.17416633733627407 1101 90-91 -0.09475634602656768 1101 92-93 -0.12328503977477112 1101 94-95 -0.10744650030743941 1101 96-97 -0.26055049599643354 1101 98-99 -0.5317843218756195 1101 100-101 -0.0034626890172475555 1102 1 0.2162495078364337 1102 2 0.1656126376127105 1102 3 0.26383608511304146 1102 4 0.008853904857581085 1102 5 0.2890650267217012 1102 6 0.3293463120116513 1102 7 0.18593826985605943 1102 8 0.1685503019827621 1102 9 0.42142148668890655 1102 10-11 0.199735241379301 1102 12-13 0.19131505105666946 1102 14-15 0.3092760249288915 1102 16-17 0.41632216349087514 1102 18-19 0.5108544884053146 1102 20-21 0.44265823373913094 1102 22-23 0.48563885439126864 1102 24-25 0.30949902019123243 1102 26-27 0.38749945129698204 1102 28-29 0.3302529476078391 1102 30-31 0.6247369580761983 1102 32-33 0.5678872017868315 1102 34-35 0.5460117707802823 1102 36-37 0.4845680175321405 1102 38-39 0.4424517064573479 1102 40-41 0.3609865326168915 1102 42-43 0.39875894294291925 1102 44-45 0.5524589214284461 1102 46-47 0.3866220014755939 1102 48-49 0.7188809635861091 1102 50-51 0.8605766368946988 1102 52-53 0.8831777420163505 1102 54-55 0.9108161339893499 1102 56-57 0.5895300235834142 1102 58-59 0.5840148623306121 1102 60-61 0.5120351821406004 1102 62-63 0.3156691387685697 1102 64-65 0.7098453884987777 1102 66-67 0.4926871194602711 1102 68-69 0.7660799517594512 1102 70-71 0.47825885295856807 1102 72-73 0.8008646134366479 1102 74-75 0.5692039503882782 1102 76-77 0.8361592536838209 1102 78-79 0.6613722892103766 1102 80-81 0.7984048100919701 1102 82-83 0.43220644309085543 1102 84-85 0.8007400376459195 1102 86-87 0.6719026470996994 1102 88-89 0.7977768128002936 1102 90-91 0.857104828649252 1102 92-93 0.6421205030678401 1102 94-95 0.92160257164781 1102 96-97 0.2170925245985309 1102 98-99 0.5258129321289573 1102 100-101 0.686377127916387 1103 1 0.1229161745031 1103 2 0.24561263761271235 1103 3 0.3771694184463712 1103 4 0.4155205715242474 1103 5 0.2801761378328109 1103 6 0.21156853423387645 1103 7 0.23482715874494886 1103 8 0.23743919087165466 1103 9 0.3458659311333534 1103 10-11 0.12529079693485556 1103 12-13 0.4202039399455586 1103 14-15 0.46816491381777325 1103 16-17 0.33965549682420715 1103 18-19 0.43418782173864656 1103 20-21 0.47821378929468494 1103 22-23 0.45119440994682947 1103 24-25 0.5483879090801196 1103 26-27 0.34972167351920547 1103 28-29 0.3724751698300608 1103 30-31 0.33029251363174694 1103 32-33 0.21455386845349977 1103 34-35 0.07934510411361373 1103 36-37 0.4545680175321465 1103 38-39 0.40911837312401644 1103 40-41 0.3520976437279941 1103 42-43 0.5098700540540335 1103 44-45 0.8302366992062247 1103 46-47 0.7343997792533727 1103 48-49 0.5588809635861125 1103 50-51 0.08057663689469763 1103 52-53 0.5076221864608002 1103 54-55 0.9130383562115796 1103 56-57 0.825085579138964 1103 58-59 0.7495704178861615 1103 60-61 0.5431462932517022 1103 62-63 0.645669138768568 1103 64-65 0.5409564996098908 1103 66-67 0.3960204527936 1103 68-69 0.10274661842611366 1103 70-71 0.2104810751807875 1103 72-73 0.15419794676997967 1103 74-75 -0.033018271833942237 1103 76-77 -0.24606296853839993 1103 78-79 -0.011961044122958953 1103 80-81 -0.23715074546358395 1103 82-83 -0.040015779131366 1103 84-85 -0.3270377401318605 1103 86-87 -0.2992084640114143 1103 88-89 -0.3744454094219236 1103 90-91 -0.6084507269063018 1103 92-93 -0.5445461635988238 1103 94-95 -0.562841872796632 1103 96-97 -0.5862408087348037 1103 98-99 -0.5552981789821523 1103 100-101 -0.5058450943058332 1104 1 0.15963599227075065 1104 2 0.08749570519054117 1104 3 1.0538402470672281E-4 1104 4 0.18849450217471997 1104 5 0.19248694522334375 1104 6 -0.07496145817311373 1104 7 0.2075328028856731 1104 8 0.1783098565259209 1104 9 0.03836909488936158 1104 10-11 0.13640697005558167 1104 12-13 0.31443577998606287 1104 14-15 0.21097939116528153 1104 16-17 0.1807159878391431 1104 18-19 0.13721743449491441 1104 20-21 0.14690525980847724 1104 22-23 0.26847864178687075 1104 24-25 0.27877768382069235 1104 26-27 0.26779810986443664 1104 28-29 -0.2843484191347585 1104 30-31 -0.08486314316400012 1104 32-33 0.18053716382176077 1104 34-35 0.22628258829483627 1104 36-37 0.11486920710440529 1104 38-39 0.27044208863907926 1104 40-41 0.34044742859259003 1104 42-43 0.2815354552182967 1104 44-45 0.41626302165623486 1104 46-47 0.23931245958746672 1104 48-49 0.18427453483389655 1104 50-51 -0.10772758988333209 1104 52-53 -0.22173493831773783 1104 54-55 -0.17812084399090367 1104 56-57 -0.037880758497074396 1104 58-59 0.0335415644313386 1104 60-61 0.007373071282586352 1104 62-63 -9.848728740493584E-4 1104 64-65 -0.3157126980615885 1104 66-67 -0.20027921817577266 1104 68-69 -0.3458360188808989 1104 70-71 -0.13798260490020553 1104 72-73 -0.2524940299447742 1104 74-75 -0.5754758775033935 1104 76-77 -0.6392140695254902 1104 78-79 -0.5616067034497085 1104 80-81 -0.4780138181034239 1104 82-83 -0.3075581734619135 1104 84-85 -0.5966732754393753 1104 86-87 -0.5690287626699799 1104 88-89 -0.44976051952063756 1104 90-91 -0.4840720885868919 1104 92-93 -0.11603692543127408 1104 94-95 -0.19678517828891273 1104 96-97 -0.5615179537613777 1104 98-99 -0.6996768173015653 1104 100-101 0.12346141037652458 1105 1 0.14571278467258963 1105 2 0.22617760936412523 1105 3 0.09764022823921437 1105 4 0.21070889544138538 1105 5 0.2759200173055021 1105 6 0.422528986211276 1105 7 0.22203996477131227 1105 8 0.41098909670969874 1105 9 0.3415909782143345 1105 10-11 0.2476213054094316 1105 12-13 0.3552981019041326 1105 14-15 0.4296244241756 1105 16-17 0.4086150071820214 1105 18-19 0.5056708725861085 1105 20-21 0.2798851640592801 1105 22-23 0.5294241651257323 1105 24-25 0.4694001501347387 1105 26-27 0.50952864150414 1105 28-29 0.628176676421397 1105 30-31 0.5538235870780781 1105 32-33 0.45579680630660135 1105 34-35 0.46597410599685674 1105 36-37 0.5790501267600163 1105 38-39 0.5283085802803242 1105 40-41 0.6237690184925953 1105 42-43 0.42507721036287904 1105 44-45 0.22000129430980309 1105 46-47 0.4710240730386843 1105 48-49 0.2091022441887489 1105 50-51 0.25818492314704855 1105 52-53 0.15672293975647023 1105 54-55 0.0667200887916124 1105 56-57 0.047933978385671594 1105 58-59 0.35375120884661015 1105 60-61 0.5567244476773183 1105 62-63 0.5943179146631081 1105 64-65 0.28387081222759036 1105 66-67 0.22875585769002527 1105 68-69 0.08019482934890121 1105 70-71 0.26334359872127777 1105 72-73 0.33232412379446075 1105 74-75 0.495112613288466 1105 76-77 0.6971291218570812 1105 78-79 0.4765747374212985 1105 80-81 0.555175054913061 1105 82-83 0.6377431662546975 1105 84-85 0.1575809039359406 1105 86-87 -0.13839867116772453 1105 88-89 -0.021422810551868565 1105 90-91 0.022472060287672235 1105 92-93 0.11174385523356634 1105 94-95 0.0115272420809589 1105 96-97 -0.04748374658791121 1105 98-99 0.2725925931459052 1105 100-101 0.8182415346960497 1106 1 0.1080885882962015 1106 2 0.19658964910696497 1106 3 0.021259456760546414 1106 4 0.11248417305681357 1106 5 -0.0033391878376889395 1106 6 0.025246695153413157 1106 7 0.22145551123536933 1106 8 0.0839142866570981 1106 9 0.19262838324063125 1106 10-11 0.060377003831412424 1106 12-13 0.07234953381529152 1106 14-15 0.03745610155723966 1106 16-17 0.3138604776671201 1106 18-19 -0.15120010929583572 1106 20-21 0.13578083910311278 1106 22-23 0.34196069347173363 1106 24-25 0.2509789052487079 1106 26-27 0.06651286125866562 1106 28-29 0.0187012234699111 1106 30-31 0.186786766505314 1106 32-33 0.2646257075339591 1106 34-35 0.18561905047377536 1106 36-37 0.3378534581451689 1106 38-39 0.27007143825811397 1106 40-41 0.5356991762950472 1106 42-43 0.8064696709122714 1106 44-45 0.3734934041870659 1106 46-47 0.34123885971314394 1106 48-49 0.2206817298696322 1106 50-51 -0.21246455084476423 1106 52-53 0.16556279948761699 1106 54-55 0.05601728341463996 1106 56-57 0.32710186266387353 1106 58-59 0.37833478570225765 1106 60-61 0.21200644650840417 1106 62-63 0.32294883225515747 1106 64-65 0.019716078153948047 1106 66-67 0.4362551271231041 1106 68-69 0.4218079211081047 1106 70-71 0.42808643916546174 1106 72-73 0.27205235290024987 1106 74-75 0.07090893123119102 1106 76-77 0.13340542226620045 1106 78-79 0.3339680746509863 1106 80-81 0.08315576794637636 1106 82-83 0.25295356952763726 1106 84-85 0.4216260529715896 1106 86-87 0.4571229536131085 1106 88-89 0.4840747055206016 1106 90-91 0.6066881619825857 1106 92-93 0.7176760586233968 1106 94-95 0.5865929931037499 1106 96-97 0.5844201108054285 1106 98-99 0.692321552818612 1106 100-101 0.6742650589508727 1107 1 0.11512174195491909 1107 2 0.05309300877616607 1107 3 0.1163224044087805 1107 4 -0.029623373277082976 1107 5 -0.06157499231232322 1107 6 0.008799083841296351 1107 7 0.15047788441708576 1107 8 0.018105381687739452 1107 9 0.15486902416214576 1107 10-11 0.18019443719181538 1107 12-13 -0.1102909446606688 1107 14-15 0.12105094760316604 1107 16-17 0.2171453849993199 1107 18-19 0.2722820401540673 1107 20-21 0.3299863327160537 1107 22-23 0.0024125874390961144 1107 24-25 0.018721004488163828 1107 26-27 0.2923721612660515 1107 28-29 0.034899628550022044 1107 30-31 0.18376860213996338 1107 32-33 0.02618841520581583 1107 34-35 0.05178145909815157 1107 36-37 -0.012110545399096395 1107 38-39 0.08274911307167088 1107 40-41 -0.056322532336714914 1107 42-43 -0.18926151863214358 1107 44-45 -0.26632052182636556 1107 46-47 -0.35167445343756043 1107 48-49 -0.08844475613789626 1107 50-51 -0.27618519988855184 1107 52-53 -0.2164011302177613 1107 54-55 -0.01166066829473067 1107 56-57 0.3690446559888372 1107 58-59 0.25311788398180823 1107 60-61 -0.11745803698860158 1107 62-63 -0.4814519652047835 1107 64-65 -0.2583773095740298 1107 66-67 -0.4753666516555981 1107 68-69 -0.43866427855223833 1107 70-71 -0.5765377209172371 1107 72-73 -0.5217525647693968 1107 74-75 -0.6779742556550268 1107 76-77 -0.7802100063685202 1107 78-79 -0.870145674149132 1107 80-81 -0.8324279284281353 1107 82-83 -0.5755094741111435 1107 84-85 -0.31977863948945995 1107 86-87 -0.44712185920532477 1107 88-89 -0.5711406271235688 1107 90-91 -0.6014890328782272 1107 92-93 -0.8990262968369898 1107 94-95 -1.0267081587828315 1107 96-97 -0.5696954839667789 1107 98-99 -0.6764782884277878 1107 100-101 -0.5285265123405694 1108 1 0.25467600887163044 1108 2 0.18813851752989663 1108 3 0.2396814957410598 1108 4 0.26229765917090475 1108 5 0.2128607479087279 1108 6 0.07359751974112072 1108 7 0.3237436528788322 1108 8 0.21026182717255182 1108 9 0.24436144528104364 1108 10-11 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#Quality Count 2 2314.0 3 466.0 4 381.0 5 447.0 6 565.0 7 624.0 8 850.0 9 1053.0 10 1260.0 11 1724.0 12 2547.0 13 3470.0 14 3640.0 15 3666.0 16 4654.0 17 6191.0 18 5227.0 19 3631.0 20 3714.0 21 3901.0 22 4349.0 23 4576.0 24 5162.0 25 5960.0 26 6723.0 27 7665.0 28 8861.0 29 10008.0 30 12324.0 31 14691.0 32 18092.0 33 22528.0 34 30656.0 35 44278.0 36 62345.0 37 87769.0 38 72167.0 39 12158.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77574695376398 19.55141044900684 15.514104490068437 19.15873810716074 2 14.953354712423472 23.55961850818375 37.41493482154013 24.072091957852653 3 22.816695508755707 25.64988411102851 31.8616579307415 19.671762449474283 4 12.639519795721565 17.299309144000883 39.453980076187854 30.607190984089694 5 11.640214946263434 39.33995667749729 34.8364992085312 14.183329167708072 6 27.79530297067636 36.939913921805015 17.048438245037595 18.216344862481026 7 23.661462660894156 35.75916219463689 22.67355536258322 17.90581978188573 8 24.13034831879489 30.385385062294233 21.132978357881058 24.35128826102982 9 23.34141333366653 19.879091541405835 24.602768024856413 32.176727100071226 10-11 24.33204855102793 25.712200434368864 27.222600507666005 22.7331505069372 12-13 28.392174583953295 24.58931559366895 27.124173138735685 19.894336683642074 14-15 23.572344176447128 26.598898507080353 29.51163815051654 20.317119165955976 16-17 19.295006949975793 29.66332963709153 30.00911045515834 21.032552957774342 18-19 18.948745129319054 30.176354575159998 31.108942893023084 19.76595740249787 20-21 21.65028323892036 26.658301399533492 29.489961679440185 22.201453682105964 22-23 20.060112852194454 26.859259691270722 30.990299876899876 22.09032757963495 24-25 22.647782750945012 27.062698363924042 29.82133054734121 20.46818833778974 26-27 21.534782124103792 25.141481574843834 33.50371190570186 19.820024395350508 28-29 19.484891283063988 29.491473875855633 31.18961616818108 19.834018672899298 30-31 20.255616608414183 27.107712239686393 30.384153936520764 22.252517215378656 32-33 20.40016787347422 27.650802345646486 31.095332763341098 20.853697017538195 34-35 22.43700058895521 26.462049981830106 30.22259165354397 20.878357775670718 36-37 21.345129594943863 25.767367396695512 32.64139294944908 20.246110058911547 38-39 21.103327495621716 27.222881318695112 31.504392930301073 20.1693982553821 40-41 20.500831407326842 27.99238660963596 31.019023017956304 20.487758965080893 42-43 19.739234314621832 28.467687775771637 28.89961232444239 22.893465585164137 44-45 20.98332044602975 26.577107754250456 29.549523344155876 22.890048455563914 46-47 22.5349902389317 28.352084767502443 29.232359458401945 19.880565535163907 48-49 21.39044372100713 27.452248428512082 29.75074898783307 21.406558862647717 50-51 19.10869146674256 28.395745090489026 31.687691483484286 20.807871959284125 52-53 20.791717634452933 29.575093468554485 28.018382933840062 21.614805963152513 54-55 19.487914021785414 27.226120737368653 29.727541047754862 23.558424193091064 56-57 21.707801281944004 27.200307934329658 28.361860492360215 22.730030291366123 58-59 23.419487634319758 26.605509384864146 28.398182643492305 21.57682033732379 60-61 20.853190552998523 27.474830680070788 31.06402640126027 20.607952365670418 62-63 20.09538894166189 27.623666668061546 30.541618368909774 21.73932602136679 64-65 19.873770486656532 29.555936251060892 29.218961426847684 21.35133183543489 66-67 20.715102837113257 27.308379463767068 28.144850085248414 23.831667613871264 68-69 19.644210358845964 27.776545013795328 29.45197593458629 23.12726869277242 70-71 22.044920041321664 27.654325639423465 27.787040613084567 22.513713706170304 72-73 20.855881925090706 25.82025244655055 30.926533888558065 22.397331739800677 74-75 19.41571189966888 29.754090812050787 28.594870420990503 22.235326867289828 76-77 20.434445955843884 26.722925604269122 28.78874123678979 24.053887203097208 78-79 20.474504286334465 26.988895804674957 29.95496602838029 22.581633880610287 80-81 21.641403073472755 26.962709743960957 28.80384356691043 22.59204361565586 82-83 20.43373549437351 26.273335593135222 30.640507367372642 22.65242154511862 84-85 20.104420612084752 27.15783416165315 29.714151190164795 23.023594036097307 86-87 20.282428660503925 27.51011662893419 29.132125057017195 23.075329653544692 88-89 20.261184191251868 26.943971272040883 28.114183592752717 24.68066094395453 90-91 20.607589108185604 25.437221373406725 29.119069047070887 24.83612047133678 92-93 21.54889660556815 26.929605884212133 29.199084045521428 22.322413464698286 94-95 20.563277984414672 26.190825042856513 29.505585475461377 23.740311497267438 96-97 18.63664810128702 26.237360576510504 30.818739521653683 24.307251800548798 98-99 20.470974109087408 26.870913827080646 28.295808830686052 24.362303233145894 100-101 20.07751216097585 24.900049282164982 29.746652004089057 25.275786552770107 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3091.0 1 1871.0 2 648.5 3 611.0 4 537.0 5 568.0 6 823.0 7 1348.0 8 1883.5 9 1993.5 10 1615.5 11 1270.0 12 1216.5 13 1211.0 14 1228.0 15 1331.5 16 1470.0 17 1506.0 18 1437.0 19 1453.0 20 1572.0 21 1687.5 22 1810.0 23 1990.5 24 2306.5 25 2704.5 26 3144.0 27 3733.5 28 4278.5 29 4830.0 30 5417.0 31 6165.0 32 7192.0 33 8140.0 34 8846.0 35 9233.0 36 9802.0 37 10562.5 38 10937.0 39 11050.0 40 11073.0 41 11095.0 42 11007.5 43 11178.0 44 11897.0 45 12548.0 46 15247.5 47 25469.5 48 45235.0 49 45215.5 50 26119.5 51 16293.5 52 12541.5 53 11189.0 54 10679.0 55 9950.5 56 8727.0 57 7466.0 58 6919.0 59 6415.5 60 5301.0 61 4378.5 62 3631.0 63 2693.5 64 1888.0 65 1441.0 66 1023.5 67 757.0 68 587.5 69 434.5 70 342.5 71 253.0 72 187.5 73 137.5 74 101.5 75 73.0 76 52.5 77 32.0 78 18.5 79 14.5 80 10.5 81 11.0 82 10.0 83 7.5 84 5.5 85 2.5 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 1.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2827485909383134 2 0.08717561413035563 3 0.09112868493817605 4 0.1061087427362324 5 0.10798124996098943 6 0.07864530343979577 7 0.08988034678833802 8 0.0869675577720493 9 0.09175285401309506 10-11 0.08363865603914789 12-13 0.09040048768410387 14-15 0.09830662929974472 16-17 0.08769575502612148 18-19 0.09778648840397888 20-21 0.09965899562873592 22-23 0.1127665462020352 24-25 0.1286828576124701 26-27 0.13097147755383978 28-29 0.12171296960920774 30-31 0.11339071527695423 32-33 0.10725305270691725 34-35 0.3797028539090669 36-37 0.5833900286909718 38-39 0.5642488437267887 40-41 0.5272148119482605 42-43 0.5538460258114718 44-45 0.5780845915541603 46-47 0.5659132945932394 48-49 0.5882793531111707 50-51 0.5806852960329895 52-53 0.583494056870125 54-55 0.5722590135215827 56-57 0.544067376971074 58-59 0.5372015171469647 60-61 0.48445923031630805 62-63 0.561960223785419 64-65 0.5947291002186672 66-67 0.6070044253587412 68-69 0.6118937497789402 70-71 0.6082527635085792 72-73 0.5980580019515686 74-75 0.595249241114433 76-77 0.5802691833163768 78-79 0.5547822794238503 80-81 0.5504130958994172 82-83 0.5661213509515458 84-85 0.5750677743587184 86-87 0.5644569000850951 88-89 0.5777725070167007 90-91 0.592752564814757 92-93 0.6004506500720915 94-95 0.5996184246388662 96-97 0.5967056356225775 98-99 0.6001385655346321 100-101 0.5780845915541603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 480639.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.680580523348105 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89894531398195 33.757467220058516 2 13.501471239057631 12.335100882388815 3 5.213058572617025 7.144066256980864 4 2.551700179164339 4.662525820230336 5 1.443612892779682 3.2972537496582883 6 0.878630958478255 2.4081823349443474 7 0.5464186846117081 1.7472505905306963 8 0.40088014645487235 1.4649950248274706 9 0.3016845904422111 1.2403014503713845 >10 1.1639429186601975 8.806229248648433 >50 0.04799872887169611 1.4763572648196368 >100 0.032913414083118965 3.105361935638009 >500 0.005485569013853161 1.6498866689177536 >1k 0.012342530281169611 13.042478365683463 >5k 4.5713075115443003E-4 1.5108125616529486 >10k+ 4.5713075115443003E-4 2.351730624648999 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11262 2.3431307072459786 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7235 1.5052877523463557 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4860 1.0111539013688027 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3568 0.742345086437014 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.7100963508995316 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3336 0.6940760113099437 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3161 0.6576661486063345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2989 0.6218804549776443 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2978 0.6195918350362747 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2969 0.6177193278115176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2892 0.6016989882219296 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2706 0.5630005055769507 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2581 0.5369934607886585 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2369 0.49288551282771476 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2251 0.4683348625475669 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2152 0.4477372830752394 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2045 0.42547525273646125 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2012 0.4186093929123521 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1839 0.3826156429253556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1798 0.37408533223479573 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1713 0.356400541778757 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1704 0.354528034554 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1680 0.3495346819546479 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1514 0.31499732647579576 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1323 0.2752585620392852 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1227 0.25528515164187676 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1184 0.2463387282347042 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1123 0.23364729037801762 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1071 0.222828359746088 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 928 0.19307630050828167 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 788 0.16394841034539437 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 747 0.1554180996548345 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 684 0.1423105490815352 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 675 0.14043804185677816 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 624 0.12982716758315493 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 622 0.12941105486654225 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 617 0.12837077307501057 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 592 0.1231693641173521 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 557 0.11588739157663028 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 537 0.11172626441050351 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 530 0.11026986990235914 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.0805635830633802E-4 0.0 16-17 0.0 0.0 0.0 4.1611271661267605E-4 0.0 18-19 0.0 0.0 0.0 4.1611271661267605E-4 0.0 20-21 0.0 0.0 0.0 5.201408957658451E-4 0.0 22-23 0.0 0.0 0.0 0.002184591762216549 0.0 24-25 0.0 0.0 0.0 0.004993352599352113 0.0 26-27 0.0 0.0 0.0 0.012691437856686619 0.0 28-29 0.0 0.0 0.0 0.03745014449514084 0.0 30-31 0.0 0.0 0.0 0.09695426297075352 0.0 32-33 0.0 0.0 0.0 0.20191869573630106 0.0 34-35 0.0 0.0 0.0 0.3500548228504137 0.0 36-37 0.0 0.0 0.0 0.5412586161339383 0.0 38-39 0.0 0.0 0.0 0.8022653176292394 0.0 40-41 0.0 0.0 0.0 1.0584867228834947 0.0 42-43 0.0 0.0 0.0 1.317828973512345 0.0 44-45 0.0 0.0 0.0 1.6189905521607693 0.0 46-47 0.0 0.0 0.0 1.9802804183597251 0.0 48-49 0.0 0.0 0.0 2.3127544789332535 0.0 50-51 0.0 0.0 0.0 2.657191780109396 0.0 52-53 0.0 0.0 0.0 2.984048319008653 0.0 54-55 0.0 0.0 0.0 3.3410730298623292 0.0 56-57 0.0 0.0 0.0 3.8067031597519136 0.0 58-59 0.0 0.0 0.0 4.2459101321365935 0.0 60-61 0.0 0.0 0.0 4.747950124729787 0.0 62-63 0.0 0.0 0.0 5.233033524121014 0.0 64-65 0.0 0.0 0.0 5.714371909062727 0.0 66-67 0.0 0.0 0.0 6.279765062760201 0.0 68-69 0.0 0.0 0.0 6.925884083480533 0.0 70-71 0.0 0.0 0.0 7.613302291324674 0.0 72-73 0.0 0.0 0.0 8.281995426921245 0.0 74-75 0.0 0.0 0.0 9.028293584166079 0.0 76-77 0.0 0.0 0.0 9.711238580306633 0.0 78-79 0.0 0.0 0.0 10.613370949922915 0.0 80-81 0.0 0.0 0.0 11.550664844092967 0.0 82-83 0.0 0.0 0.0 12.43271977513269 0.0 84-85 0.0 0.0 0.0 13.373446599214795 0.0 86-87 0.0 0.0 0.0 14.334042805515157 0.0 88-89 0.0 0.0 0.0 15.344364481450736 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 75 3.1358039E-4 31.56252 4 GTAAGCG 80 1.87174E-9 29.735365 94-95 TTTGCGT 380 0.0 28.655445 3 TATATGG 100 5.0595285E-5 28.40627 2 CGCGTTT 35 0.008325196 27.186623 68-69 GGTAAGG 90 9.036235E-4 26.340492 1 ATCTTAG 90 9.036235E-4 26.340492 1 CTTTGCG 415 0.0 26.23872 2 ATGGGCG 95 0.0012492753 24.917778 5 GAGTACT 2375 0.0 23.42027 12-13 TTGCGTT 470 0.0 23.168232 4 TACCTGG 165 2.4953988E-6 22.95456 2 CGGGTCG 85 0.0018283619 22.277107 6 GCGTATT 55 0.0028502466 21.62572 94-95 CACTACC 110 0.0029304342 21.5199 3 GTATATG 135 3.8387824E-4 21.072395 1 TACACTA 135 3.8767868E-4 21.041681 5 GTGACAC 115 0.0037918466 20.584253 3 GTACTTT 2760 0.0 20.153315 14-15 AGTACTT 2505 0.0 20.031609 12-13 >>END_MODULE