##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938955_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1020330 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.695378946027265 33.0 31.0 34.0 30.0 34.0 2 31.723359109307772 33.0 31.0 34.0 30.0 34.0 3 31.778574578812737 33.0 31.0 34.0 30.0 34.0 4 35.24548234394754 37.0 35.0 37.0 33.0 37.0 5 35.33093999000324 37.0 35.0 37.0 33.0 37.0 6 35.48915056893358 37.0 35.0 37.0 33.0 37.0 7 35.45284858820185 37.0 35.0 37.0 33.0 37.0 8 35.474575872511835 37.0 35.0 37.0 33.0 37.0 9 36.96882773220429 39.0 37.0 39.0 33.0 39.0 10-11 36.85783177991434 39.0 37.0 39.0 32.5 39.0 12-13 36.82627287250203 39.0 37.0 39.0 33.0 39.0 14-15 37.79710485823214 40.0 37.0 41.0 32.0 41.0 16-17 37.80567071437672 40.0 37.5 41.0 32.5 41.0 18-19 37.843507492673936 40.0 38.0 41.0 32.5 41.0 20-21 37.97428479021493 40.0 38.0 41.0 33.0 41.0 22-23 37.92632971685631 40.0 38.0 41.0 32.5 41.0 24-25 37.75564474238727 40.0 37.5 41.0 32.0 41.0 26-27 37.56437868140699 40.0 37.0 41.0 32.0 41.0 28-29 37.53738790391344 40.0 37.0 41.0 32.0 41.0 30-31 37.49809228386894 40.0 37.0 41.0 32.0 41.0 32-33 37.237260004116315 40.0 36.5 41.0 31.0 41.0 34-35 36.81717287544226 39.0 36.0 41.0 30.0 41.0 36-37 36.45097370458578 39.0 35.5 41.0 30.0 41.0 38-39 36.184051238324855 39.0 35.0 40.5 30.0 41.0 40-41 36.290950966844065 39.0 35.0 40.5 30.0 41.0 42-43 36.08179951584292 39.0 35.0 41.0 30.0 41.0 44-45 36.00150980565111 39.0 35.0 41.0 29.0 41.0 46-47 35.71705330628326 39.0 35.0 41.0 27.5 41.0 48-49 35.38493428596631 39.0 35.0 41.0 26.5 41.0 50-51 34.22000333225525 38.0 34.0 39.5 24.0 40.5 52-53 34.47881567728088 38.0 33.5 39.5 24.5 40.5 54-55 35.12272353062245 39.0 35.0 40.0 26.0 41.0 56-57 34.91330402908863 39.0 34.5 40.0 24.0 41.0 58-59 34.74728666215832 39.0 34.0 40.0 24.0 41.0 60-61 34.1748757754844 38.0 34.0 40.0 22.0 41.0 62-63 33.9413248654847 38.0 33.0 40.0 22.0 41.0 64-65 33.57411376711456 37.5 33.0 39.5 22.0 41.0 66-67 32.91831564297825 37.0 32.0 39.0 19.0 41.0 68-69 32.37606313643625 36.0 31.5 39.0 17.0 40.0 70-71 31.637604990542275 35.5 31.0 38.0 13.0 40.0 72-73 31.14273372340321 35.0 30.5 37.0 10.0 39.0 74-75 30.695967971146587 35.0 30.0 37.0 7.0 39.0 76-77 30.283121147079868 34.0 30.0 36.0 3.5 39.0 78-79 29.751604872933264 34.0 29.0 36.0 2.0 37.5 80-81 29.13057099173797 34.0 29.0 35.5 2.0 37.0 82-83 28.56516862191644 34.0 28.0 35.0 2.0 36.5 84-85 28.047522370213557 34.0 26.5 35.0 2.0 36.0 86-87 27.810799937275196 34.0 26.5 35.0 2.0 36.0 88-89 27.155893191418464 33.5 25.5 35.0 2.0 35.0 90-91 26.531955837817176 33.0 25.0 35.0 2.0 35.0 92-93 25.892461262532713 32.5 23.0 35.0 2.0 35.0 94-95 25.249802024835102 32.0 19.5 35.0 2.0 35.0 96-97 24.434940166416748 32.0 9.5 34.0 2.0 35.0 98-99 23.582494879107742 31.5 2.0 34.0 2.0 35.0 100-101 20.37216145756765 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1228821998809515 1101 2 0.25678905246151373 1101 3 0.32282002431936263 1101 4 0.34417773400550544 1101 5 -0.022121217553355166 1101 6 0.23047665817538388 1101 7 0.14703540055973008 1101 8 0.20150790616140313 1101 9 0.3403428347930202 1101 10-11 0.28371383323914756 1101 12-13 0.3179252236748411 1101 14-15 0.40736580705552683 1101 16-17 0.383750677901709 1101 18-19 0.6274849906792994 1101 20-21 0.417887823857761 1101 22-23 0.5980951910787908 1101 24-25 0.5062199582586544 1101 26-27 0.6624006196138481 1101 28-29 0.484679298195708 1101 30-31 0.4420159467646343 1101 32-33 0.5649333901594602 1101 34-35 0.6149234389029701 1101 36-37 0.4471121222404335 1101 38-39 0.6464146822067249 1101 40-41 0.4974652069792427 1101 42-43 0.5802370506566419 1101 44-45 0.7351209041504134 1101 46-47 0.8273629877534248 1101 48-49 0.9090957958037436 1101 50-51 1.034061353167786 1101 52-53 1.0260930002148427 1101 54-55 0.9537842994808656 1101 56-57 0.9441709734257273 1101 58-59 0.9673590897917137 1101 60-61 1.0926069222619716 1101 62-63 1.100032991226314 1101 64-65 1.0099326044802481 1101 66-67 0.9163324007106937 1101 68-69 0.9282965388062223 1101 70-71 1.029889998969523 1101 72-73 0.8495848873438518 1101 74-75 0.9472612572131887 1101 76-77 0.8025752998365974 1101 78-79 0.7730486696027228 1101 80-81 0.6795110746210504 1101 82-83 0.6627483005125967 1101 84-85 0.7998276515586866 1101 86-87 0.7354869738013221 1101 88-89 0.23094050042976377 1101 90-91 0.4848062891130773 1101 92-93 0.6384426176654223 1101 94-95 0.2934129139535173 1101 96-97 0.30018468513253893 1101 98-99 0.2808931162408861 1101 100-101 0.5477774900710521 1102 1 0.3976573562126937 1102 2 0.37629875624087816 1102 3 0.488432022625247 1102 4 0.33920750554938905 1102 5 0.36356352795599634 1102 6 0.38564993105213574 1102 7 0.45452437636879495 1102 8 0.49803248325599725 1102 9 0.5651092714257189 1102 10-11 0.5013288709829951 1102 12-13 0.562028215777282 1102 14-15 0.7342150060888883 1102 16-17 0.6884568240438611 1102 18-19 0.7017955116190322 1102 20-21 0.6147761369696951 1102 22-23 0.5951990053242966 1102 24-25 0.6587212662135116 1102 26-27 0.8169684962425663 1102 28-29 0.6915715974557841 1102 30-31 0.7056946632249321 1102 32-33 0.7866815652510155 1102 34-35 0.8515299811685964 1102 36-37 0.6684292950648683 1102 38-39 0.6716631936295343 1102 40-41 0.9238036807827754 1102 42-43 0.8187532070133017 1102 44-45 0.9233455734246903 1102 46-47 0.9576776443218549 1102 48-49 1.2093486670760711 1102 50-51 1.1642551796208593 1102 52-53 1.2095616964952711 1102 54-55 1.0330426267934527 1102 56-57 1.0180816338806409 1102 58-59 1.1713963104499285 1102 60-61 1.2661501096856753 1102 62-63 1.281696709804251 1102 64-65 1.1960334042012093 1102 66-67 1.3275035808596698 1102 68-69 1.3679088859000714 1102 70-71 1.0265266864804232 1102 72-73 1.0818265974014025 1102 74-75 1.0596606692563348 1102 76-77 1.05093225940211 1102 78-79 1.246279193532068 1102 80-81 1.4366014356165913 1102 82-83 1.4459043332986674 1102 84-85 1.350104190585565 1102 86-87 1.4216164239620142 1102 88-89 1.2639296132626683 1102 90-91 1.1752345509033582 1102 92-93 1.4344103796923768 1102 94-95 1.257267270066027 1102 96-97 1.041071353958369 1102 98-99 1.0909020850741875 1102 100-101 1.4996347859678103 1103 1 0.20926218171429056 1103 2 0.17570477657713468 1103 3 0.24335954924875125 1103 4 0.07384911653813475 1103 5 0.1368432828012658 1103 6 0.11886368270037906 1103 7 0.07031112318220067 1103 8 0.11817892784940653 1103 9 0.36623903597011775 1103 10-11 0.30597794661901645 1103 12-13 0.15678196835860092 1103 14-15 0.24518730095227426 1103 16-17 0.2670087315804537 1103 18-19 0.40266634621080044 1103 20-21 0.39645116453241513 1103 22-23 0.35811174015988456 1103 24-25 0.36472220420537127 1103 26-27 0.3729453705688428 1103 28-29 0.43600073609933077 1103 30-31 0.5724973632664287 1103 32-33 0.5213489587385993 1103 34-35 0.4642173033000532 1103 36-37 0.6621052164208763 1103 38-39 0.6269023201675807 1103 40-41 0.6798429242014876 1103 42-43 0.7407058998111395 1103 44-45 0.7683086553418264 1103 46-47 0.5587278514412617 1103 48-49 0.6377101524426649 1103 50-51 0.7174457024653762 1103 52-53 0.5813582210144261 1103 54-55 0.759236786198052 1103 56-57 0.6520591613635602 1103 58-59 0.6553473187911791 1103 60-61 0.46935732976754707 1103 62-63 0.5870382296948407 1103 64-65 0.6158958729859734 1103 66-67 0.6205953444562553 1103 68-69 0.5159575190593131 1103 70-71 0.5749769127525468 1103 72-73 0.451353648153642 1103 74-75 0.3256292440734647 1103 76-77 0.6927777461143023 1103 78-79 0.6188498068854358 1103 80-81 0.5261465095632509 1103 82-83 0.5266545057958574 1103 84-85 0.608709406507856 1103 86-87 0.7767433352432107 1103 88-89 0.5845147286577657 1103 90-91 0.6843228817443787 1103 92-93 0.6144501829596365 1103 94-95 0.756559479085972 1103 96-97 0.6151659585407749 1103 98-99 0.7705262990149073 1103 100-101 0.9581206657526167 1104 1 0.39433382658804206 1104 2 0.41403740496973995 1104 3 0.37732479868328994 1104 4 0.36149626538426105 1104 5 0.4414933610677636 1104 6 0.39605722327423365 1104 7 0.44140397411916865 1104 8 0.36078337656768156 1104 9 0.4423051533855258 1104 10-11 0.33067376770626566 1104 12-13 0.4690628794071259 1104 14-15 0.6247870392204362 1104 16-17 0.6153886331779646 1104 18-19 0.5566031088096608 1104 20-21 0.6343554058655556 1104 22-23 0.5810242419523632 1104 24-25 0.5518881858142493 1104 26-27 0.3722470574859926 1104 28-29 0.4970467958421523 1104 30-31 0.6048113643533668 1104 32-33 0.6510767311138466 1104 34-35 0.9971916591227981 1104 36-37 1.0187220793261744 1104 38-39 1.0561853171176594 1104 40-41 0.9743569187812753 1104 42-43 1.0808813758268911 1104 44-45 1.139371983119986 1104 46-47 1.1526958483371104 1104 48-49 1.1329364384172322 1104 50-51 1.1213038370596564 1104 52-53 1.2345328041052568 1104 54-55 1.4164012133222386 1104 56-57 1.363227471084386 1104 58-59 1.2928673975517384 1104 60-61 1.4347399197800357 1104 62-63 1.500736613874274 1104 64-65 1.5614401053055147 1104 66-67 1.5649917886629083 1104 68-69 1.4910803414250964 1104 70-71 1.4598884095525904 1104 72-73 1.468779542012463 1104 74-75 1.4403827497979513 1104 76-77 1.489963887823052 1104 78-79 1.7667704727350966 1104 80-81 1.6674027741967983 1104 82-83 1.7370859415733477 1104 84-85 1.6274150389240454 1104 86-87 1.505461054079106 1104 88-89 1.750112536472848 1104 90-91 1.7682867453979974 1104 92-93 1.8493081163144147 1104 94-95 1.880917651638594 1104 96-97 1.7722207282179632 1104 98-99 2.183297415002361 1104 100-101 2.443831456888919 1105 1 0.09896600885774731 1105 2 0.11213959272069474 1105 3 0.1560396818560683 1105 4 0.26051833062395247 1105 5 0.3135634098891771 1105 6 0.25935031051889723 1105 7 0.15958401192013127 1105 8 0.20273201500298654 1105 9 0.16526535576321066 1105 10-11 0.2288371415638082 1105 12-13 0.1092248371771305 1105 14-15 0.14272974909283676 1105 16-17 0.21827341904397457 1105 18-19 0.3613220872797953 1105 20-21 0.31130898670132723 1105 22-23 0.22424840212089947 1105 24-25 0.21004963601767912 1105 26-27 0.20274415983713112 1105 28-29 0.16188251462246228 1105 30-31 0.24961906267804324 1105 32-33 0.31434107942966705 1105 34-35 0.3994491715135666 1105 36-37 0.23148170020407832 1105 38-39 0.24780212521925904 1105 40-41 0.21829480097716214 1105 42-43 0.2195640899406044 1105 44-45 0.429800876868228 1105 46-47 0.38013607889748613 1105 48-49 0.5778104216907138 1105 50-51 0.16547835188743676 1105 52-53 0.02489042994525903 1105 54-55 0.3550586649904304 1105 56-57 0.2788091852456134 1105 58-59 0.35807058061988073 1105 60-61 0.3421420480831401 1105 62-63 0.3416173925138395 1105 64-65 0.44153387764917085 1105 66-67 0.3796749477664818 1105 68-69 0.49245934647874634 1105 70-71 0.46398196867193064 1105 72-73 0.41225076426288254 1105 74-75 0.41405476213714465 1105 76-77 0.25615010449371667 1105 78-79 0.11417776941006963 1105 80-81 0.18664453000629067 1105 82-83 0.4351950822909636 1105 84-85 0.6650763622358333 1105 86-87 0.8509510701983984 1105 88-89 0.8337584635988051 1105 90-91 1.003935532510905 1105 92-93 0.9560396309834402 1105 94-95 0.7107837722656107 1105 96-97 1.0022799095524917 1105 98-99 1.1653691904771613 1105 100-101 1.1167505788219394 1106 1 0.1485831398900146 1106 2 0.03414401336225126 1106 3 0.054586288567644914 1106 4 0.12906206292733913 1106 5 0.19567853379158606 1106 6 0.12709890002147262 1106 7 0.06735075094258747 1106 8 0.12729366775464968 1106 9 0.1517809673238375 1106 10-11 0.10771717962493454 1106 12-13 0.24876193631558152 1106 14-15 0.2538828933760158 1106 16-17 0.20097410724998355 1106 18-19 0.12280237445629894 1106 20-21 0.2894563260015346 1106 22-23 0.2856467521445225 1106 24-25 0.31458998951482187 1106 26-27 0.30462375847724843 1106 28-29 0.19081491977525644 1106 30-31 0.24756072210363556 1106 32-33 0.1877184429696115 1106 34-35 0.07560689172242263 1106 36-37 -0.025150638760230493 1106 38-39 0.2867206208795565 1106 40-41 0.4928040866371717 1106 42-43 0.34100067730224026 1106 44-45 0.44724225711278365 1106 46-47 0.28202294615599754 1106 48-49 -0.12500770976775755 1106 50-51 -0.1630274073913398 1106 52-53 0.06488435073714527 1106 54-55 0.36082565290799806 1106 56-57 0.22212607608307877 1106 58-59 0.13158703908467828 1106 60-61 0.454100246919495 1106 62-63 0.29826393832320974 1106 64-65 -0.11903629039908736 1106 66-67 0.2649607311198068 1106 68-69 0.21249474244773836 1106 70-71 0.3318896968652467 1106 72-73 0.23890027258489965 1106 74-75 0.3047749390509118 1106 76-77 0.15964047302368556 1106 78-79 0.45934243114949425 1106 80-81 0.650508483495944 1106 82-83 0.5985738004370695 1106 84-85 0.5557227516597756 1106 86-87 0.4176599168779056 1106 88-89 0.42240256891447814 1106 90-91 0.691718086284915 1106 92-93 0.7440652429515495 1106 94-95 0.512134083329439 1106 96-97 0.6465344521636673 1106 98-99 0.9875341815070264 1106 100-101 1.0372032595654943 1107 1 0.018003102378205682 1107 2 -7.496736776388957E-4 1107 3 0.052036902013785635 1107 4 0.10829622637342595 1107 5 0.07727681587162749 1107 6 -0.007730115080100575 1107 7 -0.05600498325053849 1107 8 0.010916462686289208 1107 9 0.09001978694120538 1107 10-11 -0.0954198780205715 1107 12-13 -0.04401396864895446 1107 14-15 -0.0028178380105003953 1107 16-17 -0.015625856717029762 1107 18-19 -0.09411678353797726 1107 20-21 -0.08000529312969462 1107 22-23 -0.21413819543579393 1107 24-25 -0.15777915915877827 1107 26-27 -0.1968404725255084 1107 28-29 -0.031514394733441975 1107 30-31 0.015139044403163382 1107 32-33 0.20291895201378907 1107 34-35 0.252693446936739 1107 36-37 0.05093834399496444 1107 38-39 0.15289657229124742 1107 40-41 0.004380467737128413 1107 42-43 0.17234698876271182 1107 44-45 -0.271869602429355 1107 46-47 -0.24413638425512119 1107 48-49 -0.06331161788986606 1107 50-51 -0.2357198116149064 1107 52-53 0.08834901705181153 1107 54-55 0.06834238586080232 1107 56-57 0.012377067771538464 1107 58-59 0.07794838338620025 1107 60-61 0.11624768852468748 1107 62-63 0.09471656274379114 1107 64-65 0.0906543986122017 1107 66-67 -0.19895211901729226 1107 68-69 0.007989784786509802 1107 70-71 -0.21184144699492435 1107 72-73 -0.012688667992939884 1107 74-75 -0.0164363111586745 1107 76-77 0.02209961046040476 1107 78-79 0.19264879180646233 1107 80-81 -0.06623103022523225 1107 82-83 -0.16136261069733493 1107 84-85 -0.03220556975455224 1107 86-87 0.10524550826164614 1107 88-89 0.35651842228725883 1107 90-91 0.39659951585759146 1107 92-93 0.1463043916563933 1107 94-95 0.32860532826945743 1107 96-97 0.4756896242003954 1107 98-99 0.3545078576328784 1107 100-101 0.15689581271156072 1108 1 0.3571443413081816 1108 2 0.2687713711645756 1108 3 0.3404087490211083 1108 4 0.2509632788509677 1108 5 0.2973860667244068 1108 6 0.14605077960701607 1108 7 0.34620803620698126 1108 8 0.4171514567694814 1108 9 0.5115482410115604 1108 10-11 0.4053144071870136 1108 12-13 0.4810004733305888 1108 14-15 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-2.3757051201924284 2316 58-59 -2.2556257660949512 2316 60-61 -2.470536480500378 2316 62-63 -2.8186262440181693 2316 64-65 -2.7677620262432114 2316 66-67 -3.0936378439933137 2316 68-69 -3.033898691464241 2316 70-71 -2.9233142467115165 2316 72-73 -3.2048117907216067 2316 74-75 -3.004708365738363 2316 76-77 -2.865360037908218 2316 78-79 -2.5880695965961493 2316 80-81 -3.041746197662114 2316 82-83 -2.900721522287956 2316 84-85 -2.972073578622954 2316 86-87 -2.987715495444135 2316 88-89 -3.2267625683627053 2316 90-91 -3.2069355059750784 2316 92-93 -3.7007144636509146 2316 94-95 -3.570010277413008 2316 96-97 -3.805255648837349 2316 98-99 -3.9791837145980367 2316 100-101 -3.4959161636128293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3502.0 3 777.0 4 847.0 5 735.0 6 892.0 7 1143.0 8 1405.0 9 1837.0 10 2342.0 11 3177.0 12 4533.0 13 6407.0 14 5705.0 15 6256.0 16 7641.0 17 10187.0 18 9169.0 19 6915.0 20 7272.0 21 7644.0 22 8751.0 23 9653.0 24 11322.0 25 13073.0 26 15055.0 27 17262.0 28 20474.0 29 23998.0 30 28768.0 31 34252.0 32 42118.0 33 53566.0 34 69936.0 35 97892.0 36 134439.0 37 181947.0 38 149202.0 39 20235.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.438316697427766 21.07353460304412 15.377535578484075 17.110613121044036 2 14.001355716719951 26.803951786711032 33.614417291960756 25.58027520460826 3 26.589350956161383 25.36584504416826 32.52812758760071 15.51667641206965 4 10.882650453231193 17.400074761807115 44.834027807075294 26.8832469778864 5 9.604439943794205 42.3827803028519 36.225851525641936 11.78692822771197 6 24.44696361992441 40.250398633369095 16.138130139820777 19.164507606885717 7 21.850517056236303 35.782547084234324 24.27599868590734 18.09093717362204 8 22.990080464448052 26.56464369597089 21.462874066519237 28.982401773061817 9 19.48664689591326 23.360380822369194 28.849412761550592 28.303559520166953 10-11 24.51409680699893 24.510909591948213 28.00468569646533 22.970307904587532 12-13 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34.67736573589099 18.89756837992629 38-39 19.996770132390107 29.253048803004173 31.85141821221904 18.89876285238668 40-41 19.21811052072473 30.552987588102614 30.604773564342107 19.62412832683055 42-43 18.181804751938937 30.516262464606676 26.37074972300874 24.931183060445647 44-45 20.94575694240615 25.67966338416322 28.50035753613282 24.874222137297817 46-47 24.57941076387367 29.53291609686098 29.052678854147633 16.83499428511772 48-49 21.679825447094807 27.84044958208764 30.102003122645087 20.377721848172463 50-51 15.198380215859583 29.651732619383232 35.416081804812265 19.733805359944917 52-53 19.76692300342494 32.54158536092853 26.608573080601772 21.082918555044763 54-55 17.520758673434464 26.9799472765891 30.300153107682128 25.199140942294314 56-57 22.42832778284357 26.090469372522218 28.439793762050403 23.0414090825838 58-59 26.469253337533967 25.879277753711655 27.855708266057576 19.795760642696806 60-61 20.960792804141164 28.372419154840866 33.24935540378294 17.417432637235027 62-63 18.263179664956322 28.929770826972888 32.16512866977221 20.641920838298585 64-65 17.769755966858067 33.332890048652956 28.840692351566126 20.05666163292285 66-67 20.572343652483685 27.54783117556742 26.170417450491573 25.70940772145732 68-69 18.152102639944864 27.844972025067825 29.89886459758416 24.104060737403152 70-71 24.010940397298793 27.951562336815268 26.905061707230082 21.13243555865586 72-73 21.070198364945984 23.97599779720385 34.24195390777121 20.711849930078955 74-75 17.561325706134124 33.619525708794086 28.346295729822575 20.472852855249215 76-77 19.63653608637328 27.048824304599368 28.318234556009276 24.99640505301808 78-79 20.140754712707853 27.286562474305175 30.746642994193646 21.826039818793326 80-81 23.28400392928276 26.782395040634594 28.33997651293389 21.59362451714876 82-83 20.250565920296953 25.592892917588117 33.24715882651004 20.909382335604892 84-85 19.298837403414463 28.31936369147191 30.73349114325855 21.648307761855072 86-87 19.05355409651684 29.73364527631428 29.60329697007898 21.6095036570899 88-89 18.81483801607552 28.784791836264322 26.775918339004235 25.62445180865592 90-91 20.413060481463894 24.71270368830739 29.26250598431371 25.611729845915004 92-93 23.128900868257972 28.357757552741 29.63992439993557 18.87341717906546 94-95 20.85958081531559 26.361355168085687 30.197296993747898 22.581767022850826 96-97 15.957072146200591 27.345178817622134 34.04777959971372 22.649969436463554 98-99 19.882677117259835 29.42455731768109 27.308578195223543 23.384187369835537 100-101 18.623241328467074 24.77492894890674 30.431438095796224 26.170391626829957 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3069.0 1 1759.0 2 455.0 3 446.5 4 378.5 5 405.0 6 647.0 7 1177.5 8 1756.5 9 1956.0 10 1684.0 11 1366.0 12 1345.5 13 1340.0 14 1333.5 15 1465.5 16 1838.0 17 1905.0 18 1591.5 19 1531.5 20 1671.0 21 1952.0 22 2274.5 23 2555.5 24 2901.0 25 3695.5 26 5297.0 27 6874.5 28 7270.0 29 8117.0 30 10463.0 31 12905.5 32 16215.5 33 21151.0 34 26751.5 35 29996.5 36 33763.0 37 38189.0 38 37178.0 39 34526.5 40 31662.0 41 28404.0 42 25680.0 43 23952.5 44 23027.0 45 23274.5 46 31942.5 47 74563.0 48 156812.0 49 152333.0 50 65984.5 51 21293.0 52 8843.0 53 5206.0 54 3408.0 55 2515.5 56 2062.0 57 1656.5 58 1396.0 59 1230.5 60 1068.5 61 865.0 62 715.5 63 588.0 64 444.5 65 355.5 66 282.0 67 240.0 68 197.5 69 136.5 70 103.5 71 87.5 72 68.0 73 52.5 74 45.5 75 38.0 76 30.0 77 21.0 78 17.5 79 16.0 80 11.0 81 9.0 82 5.5 83 5.5 84 4.5 85 1.5 86 2.0 87 2.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3286191722285927 2 0.09242107945468622 3 0.10251585271431791 4 0.10712220556094597 5 0.1193731439828291 6 0.0584124743955387 7 0.06027461703566493 8 0.06125469210941559 9 0.06292081973479169 10-11 0.06184273715366597 12-13 0.060421628296727536 14-15 0.05875550067135142 16-17 0.0602256132819774 18-19 0.06674311252241921 20-21 0.0689972851920457 22-23 0.0848254976331187 24-25 0.09408720708006234 26-27 0.08752070408593299 28-29 0.08605059147530701 30-31 0.08007213352542805 32-33 0.07232954044279792 34-35 0.32156263169758803 36-37 0.4999852988738937 38-39 0.47126909921299975 40-41 0.45191261650642434 42-43 0.48562719904344676 44-45 0.49474189722932777 46-47 0.48989052561426205 48-49 0.5062087755922103 50-51 0.5030725353562083 52-53 0.502680505326708 54-55 0.4931247733576392 56-57 0.470828065429812 58-59 0.4518146089990493 60-61 0.4106514559015221 62-63 0.4841570864328208 64-65 0.5077768957102115 66-67 0.517038605157155 68-69 0.5295835661011634 70-71 0.526888359648349 72-73 0.5151764625170289 74-75 0.5173326276792802 76-77 0.4916056569933257 78-79 0.4708770691834995 80-81 0.4780316172218792 82-83 0.48499015024550884 84-85 0.48744033792988545 86-87 0.48744033792988545 88-89 0.495868983564141 90-91 0.5072868581733361 92-93 0.5146864249801535 94-95 0.5206158791763449 96-97 0.5127262748326522 98-99 0.516303548851842 100-101 0.4939088334166397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1020330.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.583687405734803 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33829287107892 14.558143466150339 2 8.83150747625496 3.2824394852026413 3 3.2996294015294727 1.839578440583866 4 1.756069931040799 1.3053701864428993 5 1.1485583401269477 1.0672224580084413 6 0.7904781949995542 0.8813999806152719 7 0.5686851890882053 0.7397787450400477 8 0.48232828643748643 0.7170750481678373 9 0.3884326435845154 0.6496659743902051 >10 3.3360367947992735 13.209557492768742 >50 0.595809696600761 7.812533883233084 >100 0.4155336482060287 14.438549007565102 >500 0.02273275683569877 2.7829204574504405 >1k 0.01533139414500615 6.573748172296351 >5k 0.0042293501089672135 5.925421825522522 >10k+ 0.006344025163450819 24.21659537656223 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 54927 5.383258357590191 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 35957 3.52405594268521 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24855 2.435976595807239 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17950 1.7592347573824156 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15836 1.5520468867915282 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15745 1.5431282036203973 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15690 1.5377377907147687 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14797 1.4502170866288357 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14480 1.41914870679094 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13212 1.2948751874393578 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12976 1.2717454156988426 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10064 0.9863475542226534 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9368 0.9181343290896083 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8887 0.870992718042202 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8496 0.8326717826585516 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 8188 0.8024854703870316 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7778 0.762302392363255 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6702 0.6568463144276852 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5515 0.5405114031734831 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5378 0.5270843746630992 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4789 0.46935795281918596 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4532 0.44417002342379425 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4032 0.3951662697362618 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3787 0.3711544304293709 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3680 0.3606676271402389 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3439 0.33704781786284826 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3250 0.318524398968961 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3037 0.2976487998980722 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2890 0.28324169631393764 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2465 0.24158850567953505 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2459 0.24100046063528469 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2401 0.2353160252075309 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2384 0.2336498975821548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.2205168915938961 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2064 0.202287495222134 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2007 0.1967010673017553 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1947 0.19082061685925142 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1795 0.17592347573824155 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1743 0.17082708535473817 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1379 0.13515235267021453 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1323 0.1296639322572109 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1298 0.12721374457283427 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1246 0.1221173541893309 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1178 0.11545284368782649 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1168 0.11447276861407585 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1141 0.11182656591494909 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1086 0.10643615300932052 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1076 0.10545607793556987 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1065 0.10437799535444414 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4501876843766236E-4 0.0 12-13 0.0 0.0 0.0 2.940225221251948E-4 0.0 14-15 0.0 0.0 0.0 4.900375368753246E-4 0.0 16-17 0.0 0.0 0.0 5.390412905628571E-4 0.0 18-19 0.0 0.0 0.0 9.800750737506492E-4 0.0 20-21 0.0 0.0 0.0 0.001470112610625974 0.0 22-23 0.0 0.0 0.0 0.0024501876843766235 0.0 24-25 0.0 0.0 0.0 0.004018307802377662 0.0 26-27 0.0 0.0 0.0 0.006321484225691688 0.0 28-29 0.0 0.0 0.0 0.01729832505169896 0.0 30-31 0.0 0.0 0.0 0.04268226946184078 0.0 32-33 0.0 0.0 0.0 0.10148677388687974 0.0 34-35 0.0 0.0 0.0 0.18631227151999843 0.0 36-37 0.0 0.0 0.0 0.2922093832387561 0.0 38-39 0.0 0.0 0.0 0.4537257553928631 0.0 40-41 0.0 0.0 0.0 0.6259249458508522 0.0 42-43 0.0 0.0 0.0 0.8162065214195408 0.0 44-45 0.0 0.0 0.0 1.0543647643409486 0.0 46-47 0.0 0.0 0.0 1.351768545470583 0.0 48-49 0.0 0.0 0.0 1.5993355090999972 0.0 50-51 0.0 0.0 0.0 1.8741975635333668 0.0 52-53 0.0 0.0 0.0 2.145335332686484 0.0 54-55 0.0 0.0 0.0 2.452000823263062 0.0 56-57 0.0 0.0 0.0 2.9086178001234897 0.0 58-59 0.0 0.0 0.0 3.2906020601178048 0.0 60-61 0.0 0.0 0.0 3.712279360599022 0.0 62-63 0.0 0.0 0.0 4.141160212872306 0.0 64-65 0.0 0.0 0.0 4.563768584673586 0.0 66-67 0.0 0.0 0.0 5.087079670302745 0.0 68-69 0.0 0.0 0.0 5.69903854635265 0.0 70-71 0.0 0.0 0.0 6.324522458420315 0.0 72-73 0.0 0.0 0.0 6.87988199896112 0.0 74-75 0.0 0.0 0.0 7.5758823125851436 0.0 76-77 0.0 0.0 0.0 8.17691335156273 0.0 78-79 0.0 0.0 0.0 9.123126831515293 0.0 80-81 0.0 0.0 0.0 10.070173375280547 0.0 82-83 0.0 0.0 0.0 10.821302911803045 0.0 84-85 0.0 0.0 0.0 11.683033920398302 0.0 86-87 0.0 0.0 0.0 12.53266100183274 0.0 88-89 0.0 0.0 0.0 13.488381210000686 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 360 0.0 38.150715 3 GCAAGCG 50 2.993329E-8 38.056587 94-95 GTAAGCG 385 0.0 37.068104 94-95 GACATAT 415 0.0 36.602577 1 CATTCCG 220 0.0 34.416264 9 ATTGCCG 55 0.0026273206 34.416264 8 GGGATAC 55 0.0026273206 34.416264 6 GCTTTGC 1275 0.0 34.252117 1 TAGGCAT 240 0.0 33.546318 5 CTTTGCG 1320 0.0 33.00814 2 GGCATTC 235 0.0 32.21948 7 CGTCCGT 30 0.0039281882 31.705229 44-45 GTATGGT 120 1.5141813E-7 31.64598 1 TTGCGTT 1425 0.0 31.573006 4 CTGTATA 75 3.1465973E-4 31.54824 9 CCTTTAG 250 0.0 30.38014 1 GGTATGG 110 2.8140457E-6 30.11423 8 TTTGCGT 1470 0.0 29.962141 3 ACATATA 465 0.0 29.536037 2 TACCTGG 145 2.469642E-8 29.395557 4 >>END_MODULE