##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938955_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1020330 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03382239079514 33.0 31.0 34.0 30.0 34.0 2 32.2486352454598 34.0 31.0 34.0 30.0 34.0 3 32.34414650162203 34.0 31.0 34.0 30.0 34.0 4 35.849382062666 37.0 35.0 37.0 35.0 37.0 5 35.79586114296355 37.0 35.0 37.0 35.0 37.0 6 35.90566973430165 37.0 35.0 37.0 35.0 37.0 7 35.863993021865475 37.0 35.0 37.0 35.0 37.0 8 35.87278527535209 37.0 35.0 37.0 35.0 37.0 9 37.45127066733312 39.0 37.0 39.0 34.0 39.0 10-11 37.34243039016789 39.0 37.0 39.0 34.0 39.0 12-13 37.32216733801809 39.0 37.0 39.0 34.0 39.0 14-15 38.37664726118021 40.0 37.5 41.0 33.5 41.0 16-17 38.60626905020925 40.0 38.0 41.0 34.0 41.0 18-19 38.74159291601737 40.0 38.0 41.0 34.0 41.0 20-21 38.71175894073486 40.0 38.0 41.0 34.0 41.0 22-23 38.65320337537855 40.0 38.0 41.0 34.0 41.0 24-25 38.48152264463458 40.0 38.0 41.0 34.0 41.0 26-27 38.23986504366235 40.0 37.5 41.0 33.5 41.0 28-29 38.18558309566512 40.0 37.5 41.0 33.5 41.0 30-31 38.14321445022689 40.0 37.5 41.0 33.5 41.0 32-33 38.005568296531514 40.0 37.0 41.0 33.0 41.0 34-35 37.80141179814373 40.0 37.0 41.0 33.0 41.0 36-37 37.60837866180549 40.0 36.5 41.0 32.5 41.0 38-39 37.44563180539629 40.0 37.0 41.0 32.0 41.0 40-41 37.526604627914494 40.0 37.0 41.0 33.0 41.0 42-43 37.31526956964903 40.0 37.0 41.0 31.5 41.0 44-45 37.0981873511511 40.0 36.0 41.0 31.0 41.0 46-47 36.810794056824754 40.0 36.0 41.0 30.5 41.0 48-49 36.42452000823263 39.0 36.0 41.0 30.0 41.0 50-51 36.19141552242902 39.0 35.5 40.0 29.0 41.0 52-53 36.12355316417238 39.0 35.5 40.0 28.5 41.0 54-55 35.95557907735733 39.0 35.0 40.0 28.0 41.0 56-57 35.74734252643753 39.0 35.0 40.0 28.0 41.0 58-59 35.49795801358384 38.5 35.0 40.0 28.0 41.0 60-61 34.92246822106573 38.0 34.0 40.0 26.0 41.0 62-63 34.63735458136093 38.0 34.0 40.0 26.0 41.0 64-65 34.272138915840955 37.0 33.5 39.5 26.0 40.5 66-67 33.66452765281821 36.5 33.0 39.0 25.5 40.0 68-69 33.077160820518856 36.0 32.0 39.0 24.0 40.0 70-71 32.32850548352004 35.0 31.5 37.5 22.0 39.5 72-73 31.680445542128528 35.0 31.0 37.0 21.0 39.0 74-75 31.216864151794027 34.0 31.0 36.5 20.0 39.0 76-77 29.306968823811907 32.5 28.5 34.5 18.0 36.0 78-79 30.27174394558623 34.0 29.5 35.0 18.0 37.0 80-81 30.158704536767516 34.0 30.0 35.0 16.5 37.0 82-83 29.676046965197536 34.0 29.5 35.0 9.5 36.0 84-85 29.150240118393068 34.0 29.0 35.0 6.5 36.0 86-87 28.677992904256467 34.0 29.0 35.0 2.0 35.5 88-89 27.898393656954124 33.0 26.5 35.0 2.0 35.0 90-91 27.219751942998833 32.0 25.5 34.0 2.0 35.0 92-93 26.852741760018816 32.0 25.0 34.0 2.0 35.0 94-95 26.22931747571864 32.0 25.0 34.0 2.0 35.0 96-97 25.17774739545049 31.0 21.5 34.0 2.0 35.0 98-99 24.033925788715415 31.0 9.5 34.0 2.0 35.0 100-101 18.4416571109347 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.062243340649800416 1101 2 0.034913715726823114 1101 3 0.036095864375340625 1101 4 0.030046076643863273 1101 5 0.11423705191661071 1101 6 0.14352085464732056 1101 7 0.08594110201067906 1101 8 0.06107445030931302 1101 9 0.29878127500807494 1101 10-11 0.28364206277166915 1101 12-13 0.2399303133130246 1101 14-15 0.2586002859933103 1101 16-17 0.2533031497315932 1101 18-19 0.22661864789643715 1101 20-21 0.3025459349493218 1101 22-23 0.31309448959059694 1101 24-25 0.31727455448797315 1101 26-27 0.20192280540960894 1101 28-29 0.20190854990720197 1101 30-31 0.19131985790548356 1101 32-33 0.35446232498019015 1101 34-35 0.3594170200510476 1101 36-37 0.4914419077212884 1101 38-39 0.3552211431675403 1101 40-41 0.5148518314186603 1101 42-43 0.3351729662941807 1101 44-45 0.4972769895384914 1101 46-47 0.6561813366500999 1101 48-49 0.7109667003889939 1101 50-51 0.49408311236722113 1101 52-53 0.500688420435651 1101 54-55 0.6574631442624792 1101 56-57 0.6413829070315913 1101 58-59 0.6348085047789382 1101 60-61 0.5710337981435458 1101 62-63 0.7833398742782762 1101 64-65 0.5480999117046679 1101 66-67 0.5547248786816326 1101 68-69 0.7709348954306137 1101 70-71 0.594572182555801 1101 72-73 0.6744478382960217 1101 74-75 0.6050469408674672 1101 76-77 0.18138779205688849 1101 78-79 0.6964187441252996 1101 80-81 0.7030948400396824 1101 82-83 0.6568278714705933 1101 84-85 0.8311748467545677 1101 86-87 0.9918352470690017 1101 88-89 0.9258152183223061 1101 90-91 0.968153296206399 1101 92-93 0.6767032271130162 1101 94-95 0.7390355190186142 1101 96-97 0.7981471356466905 1101 98-99 0.7564861412722159 1101 100-101 1.1404814569908304 1102 1 0.11068251452059741 1102 2 0.1395102427952608 1102 3 0.17266628217349478 1102 4 0.12349882833174775 1102 5 0.1826866894262622 1102 6 0.14141068747822771 1102 7 0.22032661236220719 1102 8 0.1902455806314407 1102 9 0.29616785663691303 1102 10-11 0.3192433483044326 1102 12-13 0.32067721781986336 1102 14-15 0.3626835964893331 1102 16-17 0.32344440885614034 1102 18-19 0.3162696108003473 1102 20-21 0.35171756353997097 1102 22-23 0.31812077325486143 1102 24-25 0.3721401465555445 1102 26-27 0.44604199123885735 1102 28-29 0.39191951181457085 1102 30-31 0.40623677163028304 1102 32-33 0.38835579517127883 1102 34-35 0.3671165143084991 1102 36-37 0.362978318693159 1102 38-39 0.39045370999780715 1102 40-41 0.585538694569216 1102 42-43 0.6568887539321366 1102 44-45 0.5784748270531281 1102 46-47 0.45029553979417614 1102 48-49 0.37353926074176513 1102 50-51 0.531275120140208 1102 52-53 0.4636670696297003 1102 54-55 0.5992043435947991 1102 56-57 0.5802777474610892 1102 58-59 0.6172557505997105 1102 60-61 0.40884365887750107 1102 62-63 0.7391733529399218 1102 64-65 0.5290174731785484 1102 66-67 0.3917848452976429 1102 68-69 0.2271333557809001 1102 70-71 0.6624164238174188 1102 72-73 0.6115526490785506 1102 74-75 0.6859906614384848 1102 76-77 0.47742904869008385 1102 78-79 0.2783029465219684 1102 80-81 0.5755991376056429 1102 82-83 0.672428658734848 1102 84-85 0.6128099148927895 1102 86-87 0.893090883731599 1102 88-89 1.036085778126985 1102 90-91 1.1120980880547258 1102 92-93 1.1229674589075316 1102 94-95 0.9439347442110702 1102 96-97 1.2831978344682824 1102 98-99 1.8626434944698538 1102 100-101 2.107857325846556 1103 1 0.1636643218983238 1103 2 0.16120560169992615 1103 3 0.20640084029829353 1103 4 0.21321578560400667 1103 5 -0.06316785308171546 1103 6 0.07377705433126636 1103 7 0.2002641450507312 1103 8 0.19457949591260615 1103 9 0.1745984636551583 1103 10-11 0.17174290018005678 1103 12-13 0.25458869299623643 1103 14-15 0.35905071962900337 1103 16-17 0.3279133753216996 1103 18-19 0.23476253780149392 1103 20-21 0.2657783562167566 1103 22-23 0.25167313505453137 1103 24-25 0.27923966454558524 1103 26-27 0.39761693359655226 1103 28-29 0.41781557642305955 1103 30-31 0.36514929038398236 1103 32-33 0.5113407701131436 1103 34-35 0.493118041614629 1103 36-37 0.4997139596829072 1103 38-39 0.5207007554462066 1103 40-41 0.667294998390183 1103 42-43 0.6413689173686237 1103 44-45 0.34344954792688753 1103 46-47 0.6155097923595321 1103 48-49 0.36981921448060007 1103 50-51 0.2895439358395464 1103 52-53 0.21930115949520967 1103 54-55 0.4097109574196267 1103 56-57 0.5388986784302929 1103 58-59 0.5140698931597356 1103 60-61 0.39732195124121006 1103 62-63 0.30900463717846804 1103 64-65 0.36636673764068917 1103 66-67 0.19784643354868336 1103 68-69 0.39227885501602344 1103 70-71 0.5222707404215967 1103 72-73 0.693088573921333 1103 74-75 0.36373520843997653 1103 76-77 0.3817735225183938 1103 78-79 0.7072783009085555 1103 80-81 0.5503157142791117 1103 82-83 0.49255460010299146 1103 84-85 0.7475603034718823 1103 86-87 0.7926956748565281 1103 88-89 0.88710467792637 1103 90-91 0.8569403605487302 1103 92-93 1.0448182494480562 1103 94-95 0.7542762272695107 1103 96-97 0.47692200592926426 1103 98-99 0.619009316207201 1103 100-101 0.995266381151243 1104 1 0.02942432633282266 1104 2 0.005718572006855993 1104 3 0.09851960292848361 1104 4 0.11397218098296946 1104 5 -0.03941677307636127 1104 6 0.13570459124425582 1104 7 0.08868468015496944 1104 8 0.03739458333128454 1104 9 0.015197506622804724 1104 10-11 0.09478265281213538 1104 12-13 0.03991013994432535 1104 14-15 0.3188660854088923 1104 16-17 0.03628783019765791 1104 18-19 0.060866914820671525 1104 20-21 0.16101813849250135 1104 22-23 -0.004009470502808199 1104 24-25 0.09290695903126078 1104 26-27 -0.01736721427471366 1104 28-29 0.1830299446816852 1104 30-31 0.04852847499049062 1104 32-33 0.007988385304678047 1104 34-35 0.020815584685301758 1104 36-37 0.0039237211237335146 1104 38-39 0.194504193869939 1104 40-41 0.1590327315176978 1104 42-43 0.22408678321779973 1104 44-45 0.14281317322618037 1104 46-47 0.11101805462640613 1104 48-49 -0.14670712680471354 1104 50-51 -0.12412793774831243 1104 52-53 -0.02138307887356916 1104 54-55 0.2826505120657785 1104 56-57 0.03869984899341716 1104 58-59 0.1814493332319458 1104 60-61 0.35403437277889793 1104 62-63 0.3093366220867111 1104 64-65 0.23755779826920076 1104 66-67 0.2235056736346266 1104 68-69 0.19301862102389578 1104 70-71 0.3932364610216439 1104 72-73 0.20802488222505744 1104 74-75 0.4466615439411541 1104 76-77 0.23813853480364244 1104 78-79 0.5614375114713397 1104 80-81 0.7979866985571249 1104 82-83 0.5078512114468694 1104 84-85 0.6487764166094436 1104 86-87 0.7753902836867219 1104 88-89 0.8353144297269814 1104 90-91 0.8640825549267959 1104 92-93 0.6478112952378723 1104 94-95 0.940727025419072 1104 96-97 0.7982791795145943 1104 98-99 1.0154963013142755 1104 100-101 1.420824037470343 1105 1 0.07359536467727423 1105 2 0.09570154882682402 1105 3 0.1003609802648242 1105 4 0.027155121647510327 1105 5 -0.10192046683989986 1105 6 -0.02015195051236418 1105 7 0.07303471103773518 1105 8 0.02070398687103392 1105 9 -0.00815055541156795 1105 10-11 0.1252737292588435 1105 12-13 0.07351672486727523 1105 14-15 0.04705202164323197 1105 16-17 -0.015945752399524338 1105 18-19 0.06362550434185721 1105 20-21 0.18098821755931738 1105 22-23 -0.072275435383375 1105 24-25 0.06979935376045177 1105 26-27 -0.036311151345728376 1105 28-29 0.2180253846943927 1105 30-31 0.08326144051188322 1105 32-33 0.18233981836905144 1105 34-35 0.15395041082057048 1105 36-37 0.21438886300481386 1105 38-39 0.15422797568120927 1105 40-41 -0.010776676077959735 1105 42-43 0.04396728489751922 1105 44-45 0.03488632770604028 1105 46-47 0.27188594710109015 1105 48-49 0.28111064984513945 1105 50-51 0.4892297541448727 1105 52-53 0.5930500051959058 1105 54-55 0.6533432940752206 1105 56-57 0.49186872148875693 1105 58-59 0.41646482096516735 1105 60-61 0.2550079039936648 1105 62-63 0.2552894740198113 1105 64-65 0.16711787248053867 1105 66-67 0.42461096356753814 1105 68-69 0.07503141216488984 1105 70-71 0.4692242747887505 1105 72-73 0.5696961899969644 1105 74-75 0.5183900862169288 1105 76-77 0.4295623980805061 1105 78-79 0.6680745816766027 1105 80-81 0.7627722400947263 1105 82-83 0.707282574657107 1105 84-85 0.6262766165072371 1105 86-87 0.7255676422778663 1105 88-89 1.0285165142263857 1105 90-91 0.8282058263903131 1105 92-93 1.0797904224319659 1105 94-95 1.1189286637697826 1105 96-97 1.0787444720432866 1105 98-99 1.2960177969901103 1105 100-101 1.0181064704524339 1106 1 -0.019858143146954887 1106 2 0.017906407127313173 1106 3 -0.02686847570111439 1106 4 -0.02754894095951954 1106 5 0.04159840348464172 1106 6 0.05065981099325256 1106 7 0.12046464871483664 1106 8 0.02207511261482864 1106 9 -0.05700227890010723 1106 10-11 0.02189194948255846 1106 12-13 -0.027374451800895372 1106 14-15 0.03772836658542644 1106 16-17 -0.12401579104435001 1106 18-19 -0.14294202277505264 1106 20-21 -0.10454073411868592 1106 22-23 -0.020187524747740326 1106 24-25 0.07659330935359776 1106 26-27 -0.0020539325614095105 1106 28-29 -0.08639536699087813 1106 30-31 -0.035986301535857024 1106 32-33 -0.021051542767857256 1106 34-35 -0.0696366964124806 1106 36-37 -0.08355896373334559 1106 38-39 -0.10659868649212711 1106 40-41 0.0655658432472066 1106 42-43 0.23865007027614382 1106 44-45 0.17639751752380306 1106 46-47 0.21218856391384833 1106 48-49 -0.018754550322697128 1106 50-51 -0.13926455155339568 1106 52-53 -0.2085739803637665 1106 54-55 0.018734538473701434 1106 56-57 -0.21832044981164955 1106 58-59 -0.17281033258439038 1106 60-61 -0.20001531703951514 1106 62-63 0.031569659435660924 1106 64-65 -0.04108068855327929 1106 66-67 -0.0949939319570845 1106 68-69 -0.11752530233148661 1106 70-71 -0.05511293183714372 1106 72-73 0.06631077591148582 1106 74-75 -0.18762238276754672 1106 76-77 -0.20819774412009906 1106 78-79 -0.017758660799000126 1106 80-81 -0.13589055438068343 1106 82-83 0.0713863925539826 1106 84-85 0.036464576371248114 1106 86-87 0.18347683520792302 1106 88-89 0.3877448512656123 1106 90-91 0.7698200984326782 1106 92-93 0.781739072946884 1106 94-95 0.6559237288184114 1106 96-97 0.46172962754215874 1106 98-99 0.8697705956835193 1106 100-101 1.1786685989682155 1107 1 -0.039696077977218636 1107 2 -0.02788882339534382 1107 3 0.009594625365195952 1107 4 -0.02321665008367546 1107 5 0.0018152679682970074 1107 6 -0.014338090553358995 1107 7 0.04450271872090639 1107 8 0.005080243671834239 1107 9 0.1423695389228996 1107 10-11 -0.14153643389602166 1107 12-13 0.07627404527949011 1107 14-15 0.03889266975831163 1107 16-17 0.1388808452858683 1107 18-19 0.08648037662675989 1107 20-21 0.2168912962989964 1107 22-23 0.20109985297699495 1107 24-25 0.07162523396340958 1107 26-27 0.061618767028576826 1107 28-29 0.0696207374673179 1107 30-31 0.07765510350105131 1107 32-33 0.07269048389031951 1107 34-35 -0.08210452967342974 1107 36-37 -0.019722761819430445 1107 38-39 -0.13191238704960284 1107 40-41 0.022256472897787205 1107 42-43 -0.009588114077416776 1107 44-45 -0.22689502354183588 1107 46-47 -0.3813753805953368 1107 48-49 -0.446387588622855 1107 50-51 -0.5666872238062197 1107 52-53 -0.3875715340774093 1107 54-55 -0.42218540683112593 1107 56-57 -0.21754928120743244 1107 58-59 -0.29774068788431407 1107 60-61 -0.3124788804945595 1107 62-63 -0.11808130996649169 1107 64-65 -0.06413347480992115 1107 66-67 -0.4012676306816587 1107 68-69 -0.43449694769599034 1107 70-71 -0.3483501841233121 1107 72-73 -0.35365223298949644 1107 74-75 -0.18933395008832576 1107 76-77 -0.0838011145590869 1107 78-79 -0.03721387522136155 1107 80-81 -0.2502968795253615 1107 82-83 -0.26895202601404833 1107 84-85 -0.07365163524866603 1107 86-87 -0.043238923812563 1107 88-89 0.05348361620863429 1107 90-91 0.23488163050152266 1107 92-93 0.2652081548657108 1107 94-95 0.5443281856626498 1107 96-97 0.7735264764253991 1107 98-99 0.7074054981184545 1107 100-101 0.9825444371779817 1108 1 0.1880640513731393 1108 2 0.17326176418838912 1108 3 0.28790738730432963 1108 4 0.20794214116671128 1108 5 0.1245414988553577 1108 6 0.1125241988193153 1108 7 0.12554581856606717 1108 8 0.23456407643319466 1108 9 0.28007705487129897 1108 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-0.7824558344845478 2316 74-75 -0.9450707479898419 2316 76-77 -0.8754004124108477 2316 78-79 -1.1462801056326626 2316 80-81 -1.311063351548892 2316 82-83 -1.3827000407734928 2316 84-85 -1.613122795027195 2316 86-87 -1.5807787778899183 2316 88-89 -1.6744801140932033 2316 90-91 -1.9629187826673409 2316 92-93 -1.9983378529598177 2316 94-95 -1.8896428129083738 2316 96-97 -1.984270593180419 2316 98-99 -2.2473198719622296 2316 100-101 -1.3625562478543998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 7.0 5 25.0 6 67.0 7 123.0 8 254.0 9 481.0 10 792.0 11 1320.0 12 2011.0 13 2687.0 14 3541.0 15 4639.0 16 6084.0 17 8741.0 18 8813.0 19 6416.0 20 6018.0 21 6642.0 22 7414.0 23 8396.0 24 9671.0 25 11590.0 26 13488.0 27 16336.0 28 19871.0 29 24131.0 30 29312.0 31 35776.0 32 43948.0 33 56028.0 34 74165.0 35 104870.0 36 157658.0 37 215655.0 38 127169.0 39 6188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.61398675434134 21.72103041030843 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35.030909193371734 29.06635369525961 18.946759741357553 20-21 21.15776268462164 27.668352395793516 26.50554232454206 24.66834259504278 22-23 18.738741387590288 27.87284505993159 29.75650034792665 23.63191320455147 24-25 24.707324794257538 28.67491760010742 27.16364035521876 19.454117250416285 26-27 21.81912803658034 24.206249059740014 35.59472699750424 18.379895906175406 28-29 17.212617486499465 34.161006733115755 29.753070085168527 18.873305695216253 30-31 19.730430350964884 27.552703537090938 28.59712053943332 24.119745572510855 32-33 20.690217870688894 28.523908931424145 30.869718620446324 19.916154577440633 34-35 24.54265916872429 26.383932028414286 29.0927875270415 19.98062127581992 36-37 21.53819986687175 25.114673615216226 34.57986665607915 18.767259861832876 38-39 19.87464339432244 29.402980466266975 31.916336791609957 18.806039347800624 40-41 19.207453335790202 30.60333167363869 30.345997326192727 19.84321766437838 42-43 18.19430144113036 30.712292905200606 26.301768492921763 24.791637160747268 44-45 21.15538131699847 25.710897396630934 28.36743644716692 24.766284839203674 46-47 24.586365687868113 29.786717931855396 28.852448090811126 16.774468289465368 48-49 21.86419589130849 28.032035660082492 29.850715548997574 20.253052899611436 50-51 15.17937911939823 29.883860534633573 35.17727194766373 19.759488398304462 52-53 19.90620681544206 32.72372663746043 26.323689394607623 21.04637715248988 54-55 17.655121382297885 27.239324532259175 29.8494604686719 25.256093616771047 56-57 22.578626522791645 26.186772906804663 28.336028539786145 22.898572030617544 58-59 26.56504515241267 26.000468479558414 27.66195377537679 19.772532592652123 60-61 20.677089663121333 29.02329492663 32.9419645832435 17.357650827005177 62-63 18.240061941351733 29.164967853222517 31.882889681668498 20.712080523757255 64-65 17.66077423711471 33.767919001921456 28.345750183398255 20.22555657756558 66-67 20.564008097582633 27.822674505503013 25.81249029088865 25.800827106025704 68-69 18.159097558214583 28.259083003650403 29.341119290090106 24.240700148044912 70-71 23.925420134094004 28.502816248484557 26.444656576457206 21.127107040964237 72-73 21.17466897964384 24.422441759038744 33.6247096527594 20.778179608558016 74-75 17.57534327129458 34.24686130957632 27.465329844266073 20.712465574863035 76-77 19.81064333004054 27.447545073126424 27.61303473909627 25.128776857736764 78-79 20.25592560593906 27.977504578326005 29.92296847043972 21.843601345295216 80-81 23.34566435351821 27.268575304081587 27.59405935256999 21.791700989830215 82-83 20.351405917693295 26.27473464467378 32.443964207658304 20.929895229974615 84-85 19.27080347162703 28.998356901723316 29.909975071753852 21.8208645548958 86-87 19.070601720715537 30.335299683196464 28.491619837718506 22.102478758369497 88-89 18.744613821788437 29.47789199934443 25.693995681706213 26.083498497160925 90-91 20.659850757061836 25.435693649082815 28.03831969118351 25.86613590267184 92-93 23.080718983074103 29.3404584791195 28.445943959307286 19.132878578499113 94-95 20.777225609771037 27.439194410781308 29.07412026503411 22.709459714413548 96-97 15.739858673174364 28.56134779924142 32.835455195868 22.86333833171621 98-99 19.76046965197534 30.292552409514567 25.86285809493007 24.08411984358002 100-101 18.62594963474927 26.145748444247996 28.720757522224506 26.507544398778233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2365.0 1 1410.0 2 454.0 3 418.0 4 340.5 5 345.0 6 556.0 7 1033.0 8 1529.5 9 1720.5 10 1453.0 11 1121.0 12 1075.0 13 1047.5 14 1011.0 15 1041.5 16 1061.0 17 1152.0 18 1304.0 19 1396.5 20 1537.5 21 1830.0 22 2193.0 23 2477.0 24 2786.5 25 3582.5 26 5282.0 27 6935.0 28 7376.5 29 8219.0 30 10339.0 31 12558.5 32 16104.0 33 21427.0 34 26982.5 35 30157.0 36 34008.5 37 38366.0 38 37376.0 39 34696.0 40 31771.5 41 28206.5 42 25080.5 43 23514.0 44 23054.5 45 23049.5 46 29064.0 47 75347.0 48 160290.0 49 149789.5 50 66284.0 51 27151.0 52 12340.0 53 5557.0 54 2848.0 55 2088.5 56 1787.0 57 1497.0 58 1259.0 59 1098.0 60 949.0 61 779.5 62 615.0 63 490.0 64 374.0 65 293.0 66 226.0 67 193.5 68 144.0 69 81.0 70 54.0 71 43.5 72 31.5 73 23.5 74 17.5 75 11.0 76 11.0 77 7.5 78 5.5 79 6.0 80 2.5 81 2.5 82 2.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39173600697813454 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.310713200631167E-4 16-17 0.0021561651622514283 18-19 2.450187684376623E-4 20-21 0.0 22-23 0.0 24-25 9.800750737506493E-5 26-27 4.900375368753247E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013623043525134024 36-37 0.025579959424891947 38-39 0.011809904638695325 40-41 0.006076465457254026 42-43 0.005341409151941039 44-45 0.0017151313790636363 46-47 0.0014211088569384416 48-49 0.0028912214675644154 50-51 4.900375368753246E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.840600590005195E-4 60-61 0.0021561651622514283 62-63 9.800750737506492E-4 64-65 9.310713200631167E-4 66-67 0.0024991914380641557 68-69 0.0030872364823145452 70-71 9.800750737506493E-5 72-73 0.0 74-75 0.0 76-77 0.09124498936618546 78-79 0.0032832514970646755 80-81 3.430262758127272E-4 82-83 0.0 84-85 1.470112610625974E-4 86-87 0.09099997059774778 88-89 0.4331441788440995 90-91 0.3855125302598179 92-93 0.0 94-95 8.330638126880519E-4 96-97 0.0 98-99 0.0 100-101 3.430262758127272E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1020330.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.76613656676641 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01253816908533 12.74436595729429 2 9.816488600889802 3.291691769772483 3 3.6541679154370343 1.8379883492434037 4 1.9428138612804018 1.3029393008813595 5 1.2254866306690018 1.0273338105271457 6 0.8706307120191707 0.8758268050160697 7 0.6842504660013194 0.8030565731196173 8 0.5153042067100901 0.6911728562504479 9 0.4333988004508318 0.6539781128608251 >10 3.636337339177408 13.111067635295248 >50 0.6738377867100477 7.888860458491676 >100 0.4803943284264174 15.138859516809887 >500 0.024545695613028624 2.744262459303404 >1k 0.018117061047711602 6.954574653176648 >5k 0.004675370592957833 5.986778019815713 >10k+ 0.00701305588943675 24.94724372214177 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 59333 5.815079435084727 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34728 3.403604716121255 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23881 2.340517283623926 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17342 1.699646192898376 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 17071 1.6730861583997332 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 16932 1.6594631148745993 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 16211 1.5887997020571774 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 15875 1.5558691795791557 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 15004 1.4705046406554743 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 14004 1.3724971332804092 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13130 1.2868385718346025 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 11092 1.08709927180422 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 9340 0.9153901188831064 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 9072 0.8891241069065892 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8782 0.8607019297678202 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 8179 0.801603402820656 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7560 0.7409367557554909 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6559 0.6428312408730509 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6078 0.5956896298256447 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5529 0.541883508276734 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4973 0.48739133417619784 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4811 0.47151411798143733 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3817 0.37409465565062283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3740 0.3665480775827428 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3663 0.3590014995148628 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3449 0.33802789293659896 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3261 0.31960248155008675 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3097 0.3035292503405761 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3041 0.2980408299275724 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2913 0.28549586898356416 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2789 0.2733429380690561 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2535 0.2484490311957896 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2400 0.23521801770015585 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2200 0.21561651622514283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2154 0.21110817088588985 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2126 0.20836396067938803 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1969 0.19297678202150284 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1943 0.19042858682975114 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1814 0.1777856183783678 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1473 0.14436505836347063 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1388 0.13603442023659013 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1277 0.12515558691795792 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1250 0.12250938421883117 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1214 0.11898111395332882 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1209 0.1184910764164535 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1164 0.11408073858457557 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1162 0.11388472356982544 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1046 0.10251585271431791 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1041 0.1020258151774426 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1037 0.10163378514794234 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4501876843766236E-4 0.0 12-13 0.0 0.0 0.0 2.940225221251948E-4 0.0 14-15 0.0 0.0 0.0 4.900375368753246E-4 0.0 16-17 0.0 0.0 0.0 5.390412905628571E-4 0.0 18-19 0.0 0.0 0.0 9.800750737506492E-4 0.0 20-21 0.0 0.0 0.0 0.001470112610625974 0.0 22-23 0.0 0.0 0.0 0.0023521801770015585 0.0 24-25 0.0 0.0 0.0 0.003920300295002597 0.0 26-27 0.0 0.0 0.0 0.006370487979379221 0.0 28-29 0.0 0.0 0.0 0.01754334382013662 0.0 30-31 0.0 0.0 0.0 0.043270314506091166 0.0 32-33 0.0 0.0 0.0 0.10354493154175609 0.0 34-35 0.0 0.0 0.0 0.18993854929287585 0.0 36-37 0.0 0.0 0.0 0.29715876236119687 0.0 38-39 0.0 0.0 0.0 0.4606352846628052 0.0 40-41 0.0 0.0 0.0 0.6356276890809835 0.0 42-43 0.0 0.0 0.0 0.8282124410729862 0.0 44-45 0.0 0.0 0.0 1.0699969617672713 0.0 46-47 0.0 0.0 0.0 1.3695079043054699 0.0 48-49 0.0 0.0 0.0 1.619574059372948 0.0 50-51 0.0 0.0 0.0 1.8965432752148814 0.0 52-53 0.0 0.0 0.0 2.171503337155626 0.0 54-55 0.0 0.0 0.0 2.481305067968206 0.0 56-57 0.0 0.0 0.0 2.93998020248351 0.0 58-59 0.0 0.0 0.0 3.325198710221203 0.0 60-61 0.0 0.0 0.0 3.7502572697068595 0.0 62-63 0.0 0.0 0.0 4.183744474826772 0.0 64-65 0.0 0.0 0.0 4.6117432595336805 0.0 66-67 0.0 0.0 0.0 5.139562690502093 0.0 68-69 0.0 0.0 0.0 5.755784893122813 0.0 70-71 0.0 0.0 0.0 6.385532131761293 0.0 72-73 0.0 0.0 0.0 6.946870130251977 0.0 74-75 0.0 0.0 0.0 7.648309860535317 0.0 76-77 0.0 0.0 0.0 8.253506218576344 0.0 78-79 0.0 0.0 0.0 9.201140807385846 0.0 80-81 0.0 0.0 0.0 10.149461448746974 0.0 82-83 0.0 0.0 0.0 10.901081022806348 0.0 84-85 0.0 0.0 0.0 11.766536316681858 0.0 86-87 0.0 0.0 0.0 12.61944664961336 0.0 88-89 0.0 0.0 0.0 13.574823831505494 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTGG 20 0.0020823947 71.17757 4 GTATGCG 20 5.416262E-4 47.45171 94-95 GTACGCG 20 5.416262E-4 47.45171 94-95 GTATGGT 140 0.0 44.24225 1 GTAAGCG 695 0.0 43.355156 94-95 GGTGTAC 55 4.9927992E-5 43.137913 8 GTGTACG 55 4.9927992E-5 43.137913 9 CGAGACA 50 6.4392225E-10 42.706535 94-95 TACAATC 75 6.970002E-6 37.96137 7 CATTCCG 225 0.0 35.852398 9 GATGTTC 55 0.0025923902 34.51033 8 GGCATTC 235 0.0 34.326763 7 CCCTATA 250 0.0 34.16523 2 CCTTTAG 240 0.0 33.748894 1 CTCCGAC 150 0.0 33.216198 94-95 GCTTTGC 1035 0.0 32.22401 1 TCTCGCC 60 0.0039704684 31.634468 3 CTTTGCG 1055 0.0 31.034765 2 GGACGTG 140 1.722583E-8 30.504667 6 GACGTGA 80 4.520137E-4 29.657318 7 >>END_MODULE