##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938954_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1030038 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7182074836074 33.0 31.0 34.0 30.0 34.0 2 31.722401503633847 33.0 31.0 34.0 30.0 34.0 3 31.782411911016876 33.0 31.0 34.0 30.0 34.0 4 35.256789555336795 37.0 35.0 37.0 33.0 37.0 5 35.33059557026051 37.0 35.0 37.0 33.0 37.0 6 35.48747910271272 37.0 35.0 37.0 33.0 37.0 7 35.439190592968416 37.0 35.0 37.0 33.0 37.0 8 35.45411043087731 37.0 35.0 37.0 33.0 37.0 9 36.95861511905386 39.0 37.0 39.0 33.0 39.0 10-11 36.84238348488114 39.0 37.0 39.0 32.5 39.0 12-13 36.80039765523213 39.0 37.0 39.0 32.0 39.0 14-15 37.77734996184607 40.0 37.0 41.0 32.0 41.0 16-17 37.79959817016459 40.0 37.5 41.0 32.5 41.0 18-19 37.86856892658329 40.0 38.0 41.0 32.5 41.0 20-21 37.69966836174976 40.0 37.5 41.0 32.0 41.0 22-23 37.84262716521138 40.0 38.0 41.0 32.5 41.0 24-25 37.70340608793074 40.0 37.5 41.0 32.0 41.0 26-27 37.55479555123209 40.0 37.0 41.0 32.0 41.0 28-29 37.52766354251008 40.0 37.0 41.0 32.0 41.0 30-31 37.49804424691128 40.0 37.0 41.0 32.0 41.0 32-33 37.26439024579676 40.0 36.5 41.0 31.0 41.0 34-35 36.85063075342851 39.0 36.0 41.0 30.0 41.0 36-37 36.48398311518604 39.0 35.5 41.0 30.0 41.0 38-39 36.25032911407152 39.0 35.0 40.5 30.0 41.0 40-41 36.346259070053726 39.0 35.0 41.0 30.0 41.0 42-43 36.1487532498801 39.0 35.0 41.0 30.0 41.0 44-45 36.058539102440875 39.0 35.0 41.0 29.5 41.0 46-47 35.77603399097897 39.0 35.0 41.0 27.5 41.0 48-49 35.50784631246614 39.0 35.0 41.0 26.5 41.0 50-51 34.282193957892815 38.0 34.0 40.0 24.5 40.5 52-53 34.57734229222611 38.0 34.0 39.5 25.0 40.5 54-55 35.21905114180254 39.0 35.0 40.0 26.0 41.0 56-57 35.015310600191455 39.0 34.5 40.5 24.5 41.0 58-59 34.853246190917226 39.0 34.0 40.0 24.5 41.0 60-61 34.339750572308986 38.0 34.0 40.0 22.0 41.0 62-63 34.12670115083132 38.0 34.0 40.0 22.0 41.0 64-65 33.72682027265013 37.5 33.5 39.5 22.0 41.0 66-67 33.123654175865354 37.0 32.0 39.0 20.5 41.0 68-69 32.63120341191296 36.0 32.0 39.0 19.0 40.0 70-71 31.921625221593768 35.5 31.0 38.0 15.5 40.0 72-73 31.423034878324877 35.0 31.0 37.0 12.5 39.0 74-75 30.970708362215763 35.0 31.0 37.0 8.0 39.0 76-77 30.579437360563396 34.5 30.5 36.5 7.0 39.0 78-79 30.038991279933363 34.0 29.5 36.0 2.0 37.5 80-81 29.43495822484219 34.0 29.0 35.5 2.0 37.0 82-83 28.952064389857462 34.0 29.0 35.0 2.0 37.0 84-85 28.51751634405721 34.0 29.0 35.0 2.0 36.0 86-87 28.329557744471565 34.0 29.0 35.0 2.0 36.0 88-89 27.736304388770122 34.0 26.5 35.0 2.0 35.5 90-91 27.171035437527546 33.0 25.5 35.0 2.0 35.0 92-93 26.621144559715272 33.0 25.0 35.0 2.0 35.0 94-95 26.033787588419067 33.0 24.0 35.0 2.0 35.0 96-97 25.296316737829088 32.0 19.5 35.0 2.0 35.0 98-99 24.602137008537547 32.0 9.5 35.0 2.0 35.0 100-101 21.896059659934878 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24806326355254527 1101 2 0.14632549632512593 1101 3 0.23234027458484263 1101 4 0.05651948831459208 1101 5 0.039145193698963965 1101 6 0.06938327869504235 1101 7 0.19343058749338837 1101 8 0.11512324243315675 1101 9 0.20248984952445426 1101 10-11 0.3484726540882548 1101 12-13 0.3299012670889354 1101 14-15 0.07339315672309965 1101 16-17 0.2902710286398289 1101 18-19 0.3428495963386169 1101 20-21 0.6290090146753542 1101 22-23 0.4511786993651512 1101 24-25 0.6214115663485487 1101 26-27 0.5782004157856093 1101 28-29 0.6189985505449513 1101 30-31 0.597516490024482 1101 32-33 0.514726732287734 1101 34-35 0.4305353676019976 1101 36-37 0.3399655728526554 1101 38-39 0.5089050364885424 1101 40-41 0.3945882308349198 1101 42-43 0.33106768978770873 1101 44-45 0.6458968673633123 1101 46-47 0.8331757422984154 1101 48-49 0.7117972910151806 1101 50-51 0.7506044190299548 1101 52-53 0.545149049010142 1101 54-55 0.7303770671836887 1101 56-57 0.49047588183857727 1101 58-59 0.44653239742336837 1101 60-61 0.28789940656566415 1101 62-63 0.3699286830865134 1101 64-65 0.39945314979079427 1101 66-67 0.4450557153656334 1101 68-69 0.3827841326461865 1101 70-71 0.20742810607383433 1101 72-73 0.3390786373335217 1101 74-75 0.12449422438953306 1101 76-77 0.37408346308316354 1101 78-79 0.3589092758980108 1101 80-81 0.18074371773639086 1101 82-83 0.2975384321694712 1101 84-85 0.07074468579670068 1101 86-87 -0.07462138141782049 1101 88-89 0.12673774988189024 1101 90-91 0.16300616172490479 1101 92-93 0.49593486548968 1101 94-95 0.6744527992162581 1101 96-97 0.4588726833420331 1101 98-99 0.10426976891500317 1101 100-101 0.9722262035535145 1102 1 0.2962754698026657 1102 2 0.11934990309831406 1102 3 0.13725455975369627 1102 4 0.22475393460441495 1102 5 0.28244216027209035 1102 6 0.3612659028288263 1102 7 0.26102527470106907 1102 8 0.16034545192860605 1102 9 0.3522617220785236 1102 10-11 0.21500199089599903 1102 12-13 0.3186154014455056 1102 14-15 0.5398260882085069 1102 16-17 0.6006758832682308 1102 18-19 0.4234578089266634 1102 20-21 0.5350722890200856 1102 22-23 0.43531537061345205 1102 24-25 0.34429399003561656 1102 26-27 0.6087524317068116 1102 28-29 0.5198042747302836 1102 30-31 0.5269532824953203 1102 32-33 0.5003578053457929 1102 34-35 0.5450709755282404 1102 36-37 0.602949269853589 1102 38-39 0.5153777948261649 1102 40-41 0.3642491895428961 1102 42-43 0.29374403516693093 1102 44-45 0.48646537241265975 1102 46-47 0.39500281405833704 1102 48-49 0.4936450517390867 1102 50-51 0.5309488294302795 1102 52-53 0.33544838276345956 1102 54-55 0.4054261050394814 1102 56-57 0.5913518816858527 1102 58-59 0.497705368616316 1102 60-61 0.48045531688007515 1102 62-63 0.5340784310963613 1102 64-65 0.29154540777525284 1102 66-67 0.247366340950272 1102 68-69 0.39229520971316845 1102 70-71 0.3833278493111436 1102 72-73 0.3652840618032904 1102 74-75 0.08689674524553226 1102 76-77 0.2618935589157836 1102 78-79 0.08890517151300159 1102 80-81 0.1964513423474301 1102 82-83 -0.15988394245588466 1102 84-85 -0.1654012250904202 1102 86-87 -0.1417596944201307 1102 88-89 -0.1276261420296052 1102 90-91 -0.007534614862915845 1102 92-93 -0.023884899510417057 1102 94-95 0.08607190751475358 1102 96-97 0.30033186380134325 1102 98-99 0.8626223117246958 1102 100-101 1.1205585585160023 1103 1 0.23198571315297656 1103 2 0.42906269313792933 1103 3 0.5125092286840882 1103 4 0.5233815520350902 1103 5 0.5622242768538683 1103 6 0.49670025483787583 1103 7 0.3543968083739628 1103 8 0.2799974043904072 1103 9 0.4610902449987293 1103 10-11 0.48203297561586567 1103 12-13 0.7348505457579009 1103 14-15 0.5378700893403661 1103 16-17 0.6529466812308797 1103 18-19 0.5125636380155143 1103 20-21 0.600571723089125 1103 22-23 0.6329207752540924 1103 24-25 0.7140449804147906 1103 26-27 0.7474019789620385 1103 28-29 0.5223191304178911 1103 30-31 0.5606332485394603 1103 32-33 0.4149800464323832 1103 34-35 0.504341632008142 1103 36-37 0.6249498357845411 1103 38-39 0.5833390285556561 1103 40-41 0.5471014815632671 1103 42-43 0.6001814997962498 1103 44-45 0.5197704091981663 1103 46-47 0.34588708117775013 1103 48-49 0.19221253900563795 1103 50-51 0.2811858130182756 1103 52-53 0.23012863177307707 1103 54-55 0.22798199072142467 1103 56-57 0.2831812535024909 1103 58-59 0.10331162215338452 1103 60-61 0.34754289469558586 1103 62-63 0.35207149844214314 1103 64-65 0.06719976544361117 1103 66-67 0.1908652090883649 1103 68-69 0.23755907502160767 1103 70-71 0.12975894721719783 1103 72-73 0.2898631506544511 1103 74-75 -0.036154330023283165 1103 76-77 0.08076381924402298 1103 78-79 0.26093403399177717 1103 80-81 -0.013968861387716913 1103 82-83 0.16725598397309227 1103 84-85 0.03495601882582022 1103 86-87 0.26939268248989023 1103 88-89 0.2598066536693153 1103 90-91 0.388100642635667 1103 92-93 0.3456786262394438 1103 94-95 0.18898998900881026 1103 96-97 0.03920778343914222 1103 98-99 0.039401457168949605 1103 100-101 0.3021693395007219 1104 1 0.19873161015354057 1104 2 0.23755165748428198 1104 3 0.23839491063088758 1104 4 0.3041398995166986 1104 5 0.297266721675598 1104 6 0.16981853440777428 1104 7 0.3117708887361559 1104 8 0.21955597824439366 1104 9 0.28243716067501623 1104 10-11 0.28177830668547443 1104 12-13 0.3332206646033953 1104 14-15 0.47677784259446554 1104 16-17 0.34308816396998765 1104 18-19 0.40135254576877344 1104 20-21 0.5152915872657005 1104 22-23 0.5098987039467886 1104 24-25 0.5237676742461446 1104 26-27 0.45079190539102143 1104 28-29 0.5055279589408102 1104 30-31 0.6269094228461967 1104 32-33 0.6125069281528042 1104 34-35 0.4222420281598147 1104 36-37 0.5209317259939397 1104 38-39 0.2945444614928334 1104 40-41 0.3879334000692083 1104 42-43 0.34260368428974175 1104 44-45 0.4166408110091524 1104 46-47 0.39039755090044537 1104 48-49 0.641210841212768 1104 50-51 0.5037777767986924 1104 52-53 0.596676452938901 1104 54-55 0.6118076839868465 1104 56-57 0.9260448641419998 1104 58-59 0.6872448423005224 1104 60-61 0.6061790010906023 1104 62-63 0.5692757995174134 1104 64-65 0.6983655832138496 1104 66-67 0.8336382707748342 1104 68-69 0.7380627535728195 1104 70-71 0.766595393170789 1104 72-73 0.5131568688208361 1104 74-75 0.4156906048946567 1104 76-77 0.6237575940035036 1104 78-79 0.5421507855480847 1104 80-81 0.528249587961465 1104 82-83 0.4535151803511326 1104 84-85 0.6096645643832623 1104 86-87 0.7832183757553075 1104 88-89 0.974764208847585 1104 90-91 0.9975969640844511 1104 92-93 0.8656107145246743 1104 94-95 1.0303482233042267 1104 96-97 1.1334239690644985 1104 98-99 1.125451259093115 1104 100-101 1.615887505884423 1105 1 0.02574915401319089 1105 2 0.06100644830749147 1105 3 0.20746373654182548 1105 4 0.1576081856165672 1105 5 0.19084296998868666 1105 6 0.109818534407772 1105 7 0.09479383070646463 1105 8 0.1469783804036382 1105 9 0.12276104731469673 1105 10-11 0.07134645783257554 1105 12-13 0.2328158063038117 1105 14-15 0.21586016378205386 1105 16-17 0.3413337780050725 1105 18-19 0.24837008962841622 1105 20-21 0.195534502245458 1105 22-23 0.2760390548239826 1105 24-25 0.29253960407070423 1105 26-27 0.1618850227999289 1105 28-29 0.26691797243608306 1105 30-31 0.3363156306734538 1105 32-33 0.20532744097332056 1105 34-35 0.17909762869962265 1105 36-37 0.34588449252564857 1105 38-39 0.4040586315333172 1105 40-41 0.37495094392885875 1105 42-43 0.3606198786217263 1105 44-45 0.03072987983505726 1105 46-47 0.3997497776210892 1105 48-49 0.25963189384435026 1105 50-51 -0.03543949715541572 1105 52-53 0.14204757304685955 1105 54-55 0.09124088236741557 1105 56-57 0.06945916913524997 1105 58-59 0.08257547657852626 1105 60-61 0.18545025615132715 1105 62-63 0.15440400464562032 1105 64-65 0.2141145710681016 1105 66-67 0.06181640842665814 1105 68-69 0.16545816517876233 1105 70-71 -0.13089448537171933 1105 72-73 0.35477630201921784 1105 74-75 0.1253262324250244 1105 76-77 -0.06895495660378614 1105 78-79 -0.015501036314258698 1105 80-81 -0.15372072242989887 1105 82-83 -0.4800475726853044 1105 84-85 -0.37395216975978585 1105 86-87 -0.32145098996668864 1105 88-89 -0.19496588561935368 1105 90-91 -0.18646511418815237 1105 92-93 -0.08703435969934858 1105 94-95 0.11478816932311986 1105 96-97 0.14524583141267655 1105 98-99 0.2956132024129552 1105 100-101 0.3207053196496048 1106 1 0.21437778264181873 1106 2 0.2773335446345868 1106 3 0.3212082502863325 1106 4 0.33703068503906053 1106 5 0.2984120775577992 1106 6 0.22657529116452935 1106 7 0.19533129124392445 1106 8 0.18770834113360024 1106 9 0.31140507595872435 1106 10-11 0.37521648170259425 1106 12-13 0.5403140738020724 1106 14-15 0.509837217759106 1106 16-17 0.5120444887157873 1106 18-19 0.34582754708588226 1106 20-21 0.4750155217264691 1106 22-23 0.37315617194110473 1106 24-25 0.36351057504167983 1106 26-27 0.2763163772312751 1106 28-29 0.40023898575709893 1106 30-31 0.28610388046170954 1106 32-33 0.513347768993647 1106 34-35 0.4179487875507846 1106 36-37 0.1923478789890396 1106 38-39 0.3012327287074186 1106 40-41 0.4194754684533848 1106 42-43 0.5505205485223925 1106 44-45 0.6008692499744299 1106 46-47 0.5655648234361408 1106 48-49 0.42663890085135137 1106 50-51 0.4966464349305113 1106 52-53 0.4567407277400193 1106 54-55 0.3508435619700947 1106 56-57 0.30659014626623105 1106 58-59 0.39046490446795445 1106 60-61 0.680380571081642 1106 62-63 0.45496148520309987 1106 64-65 0.36827180522532643 1106 66-67 0.6554685220787704 1106 68-69 0.6743716940922866 1106 70-71 0.6501357756585406 1106 72-73 0.5548733221162365 1106 74-75 0.571600198698988 1106 76-77 0.48160175395292626 1106 78-79 0.6810170202037966 1106 80-81 0.8827988740897013 1106 82-83 1.0006038479661186 1106 84-85 0.9551584793508603 1106 86-87 1.066078077562377 1106 88-89 1.1657609951075294 1106 90-91 0.933370105647068 1106 92-93 0.6908512181862285 1106 94-95 0.7723749869099343 1106 96-97 1.0238860100528555 1106 98-99 1.0785438253435764 1106 100-101 1.2144929534372402 1107 1 0.09818194971211724 1107 2 -0.03988891976529629 1107 3 0.04691783083462653 1107 4 0.12170144450821141 1107 5 0.02795008330547688 1107 6 0.06054434085938709 1107 7 -0.025777922808835285 1107 8 0.0594328882613695 1107 9 0.06377841704173903 1107 10-11 0.20179104013199378 1107 12-13 0.13323764253209447 1107 14-15 0.008266594716708653 1107 16-17 0.14273162746744106 1107 18-19 0.2511053046821843 1107 20-21 0.22073301341171003 1107 22-23 0.07021916235086678 1107 24-25 0.05853422772661787 1107 26-27 0.2881613875641946 1107 28-29 0.2913715497727267 1107 30-31 0.34352975391580287 1107 32-33 0.2017407991205502 1107 34-35 0.15017496534147767 1107 36-37 -0.06893457281760362 1107 38-39 0.16509306081371733 1107 40-41 0.06312970736971835 1107 42-43 0.23348123097904505 1107 44-45 0.4433927210261217 1107 46-47 0.2535819736508671 1107 48-49 0.1822327002959625 1107 50-51 0.2646871571042979 1107 52-53 0.2356057823107207 1107 54-55 0.17240403373217816 1107 56-57 0.25236808145947975 1107 58-59 0.2627202243039193 1107 60-61 0.04918940007192418 1107 62-63 -0.017518555321295537 1107 64-65 0.10100352888447617 1107 66-67 0.09768644564750417 1107 68-69 0.14888299975278585 1107 70-71 0.11537722678708917 1107 72-73 0.1495405700092931 1107 74-75 0.3839397559982203 1107 76-77 0.4099640880612263 1107 78-79 0.2863708619487646 1107 80-81 0.3714478052789545 1107 82-83 0.34057587644620924 1107 84-85 0.19389419086883208 1107 86-87 0.2674437577587021 1107 88-89 0.38430262141125127 1107 90-91 0.5083291372593237 1107 92-93 0.0026342714007334678 1107 94-95 -0.07179630131377124 1107 96-97 0.27727901999828575 1107 98-99 0.30327914534099065 1107 100-101 0.6433991782104016 1108 1 0.07285232861636715 1108 2 0.015026594827638462 1108 3 0.033348962427048434 1108 4 0.11416496217334071 1108 5 -0.07861673697102134 1108 6 0.0630725026617398 1108 7 0.0713322922449251 1108 8 -0.020732242306984006 1108 9 0.12983064438429182 1108 10-11 0.1876941379302508 1108 12-13 0.11477246076045589 1108 14-15 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72-73 -3.100155824677614 2316 74-75 -3.33322580377407 2316 76-77 -3.4916397228179648 2316 78-79 -3.0133290906129027 2316 80-81 -3.487023889850711 2316 82-83 -3.5926870900307044 2316 84-85 -3.760407675038824 2316 86-87 -3.8349653187751365 2316 88-89 -3.961180063598235 2316 90-91 -4.252196637566737 2316 92-93 -4.494606003741584 2316 94-95 -4.86943505842952 2316 96-97 -5.038464575827142 2316 98-99 -5.47117412790379 2316 100-101 -5.920923639626412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3754.0 3 833.0 4 755.0 5 843.0 6 952.0 7 1104.0 8 1394.0 9 1806.0 10 2294.0 11 3093.0 12 4404.0 13 6343.0 14 6253.0 15 5930.0 16 7404.0 17 9935.0 18 8970.0 19 6622.0 20 6703.0 21 7263.0 22 7978.0 23 9088.0 24 10436.0 25 12056.0 26 14058.0 27 16365.0 28 19413.0 29 22689.0 30 27340.0 31 32731.0 32 40647.0 33 51469.0 34 68063.0 35 95157.0 36 136353.0 37 191423.0 38 164222.0 39 23891.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.49181815261294 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26.302829687707437 25.081362795092655 44-45 20.885977386550753 25.709922784653788 28.52583958325095 24.87826024554451 46-47 24.578650588483377 29.573827914496135 29.02127053053319 16.826250966487297 48-49 21.638516993796557 27.915680850378234 29.975129600462452 20.47067255536276 50-51 15.174201246059077 29.573496688599466 35.47314021142582 19.779161853915635 52-53 19.770826376398954 32.511780408882224 26.565529927999393 21.151863286719433 54-55 17.57094313077768 26.99985892164391 30.28971831680341 25.139479630774996 56-57 22.567389634436182 25.94504586018719 28.43814755492047 23.049416950456163 58-59 26.459061178258423 25.873060103662848 27.91659732873694 19.751281389341788 60-61 20.930822420278773 28.337863096445908 33.29844147533051 17.432873007944806 62-63 18.259996056050937 28.946731305779057 32.154411463224626 20.63886117494538 64-65 17.807515535501803 33.297510657693586 28.808999385269214 20.0859744215354 66-67 20.554066697789438 27.542186654582178 26.15289353863944 25.75085310898895 68-69 18.125734946536912 27.833035773583724 29.88243785428818 24.15879142559118 70-71 23.99146233200805 27.9305872552208 26.83505659061677 21.24289382215438 72-73 21.018341653580833 24.013826063858875 34.26594323481848 20.701889047741815 74-75 17.6165909363895 33.600122652776435 28.341531343350628 20.44175506748344 76-77 19.62021917679819 27.0240266511488 28.35287386398697 25.002880308066032 78-79 20.17103261392128 27.276534288276004 30.695722329952087 21.856710767850632 80-81 23.192287588270517 26.768594948551872 28.332659006509164 21.706458456668443 82-83 20.255788082780406 25.58588611323853 33.285936243836794 20.87238956014427 84-85 19.38656009013508 28.268653699985503 30.698795839453723 21.645990370425697 86-87 19.00209842537557 29.760396821561603 29.64227192254131 21.59523283052152 88-89 18.843196784254797 28.723929309453478 26.75021394527785 25.682659961013876 90-91 20.460417744880722 24.778579589285886 29.21362797465017 25.547374691183222 92-93 23.170265449390882 28.346340579727837 29.63049589323234 18.852898077648938 94-95 20.851879991484328 26.40010585999527 30.237677173889693 22.51033697463071 96-97 16.002486281992816 27.39963809265258 34.09931846967683 22.498557155677776 98-99 19.91068276512331 29.4595506501281 27.256698182258525 23.373068402490063 100-101 18.497311868638175 24.93852666090296 30.424775037113445 26.139386433345425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3210.0 1 1851.0 2 484.0 3 473.5 4 374.0 5 365.5 6 638.0 7 1192.5 8 1711.0 9 1887.0 10 1648.5 11 1386.0 12 1378.5 13 1351.0 14 1360.5 15 1475.5 16 1881.0 17 2003.0 18 1737.0 19 1629.5 20 1701.0 21 1968.0 22 2299.0 23 2617.0 24 2922.0 25 3667.0 26 5302.5 27 6881.5 28 7275.5 29 8133.5 30 10476.5 31 12841.0 32 16106.5 33 21409.5 34 27132.5 35 30334.5 36 34133.0 37 38331.5 38 37540.5 39 35128.5 40 32121.5 41 28690.0 42 25845.5 43 24196.0 44 23454.5 45 23518.0 46 31836.5 47 75536.0 48 160011.0 49 154384.0 50 65467.0 51 20825.0 52 8711.0 53 5069.0 54 3441.0 55 2584.0 56 2062.0 57 1671.5 58 1394.5 59 1173.0 60 1008.0 61 908.0 62 767.5 63 593.5 64 455.5 65 363.0 66 273.5 67 230.0 68 189.5 69 124.5 70 91.5 71 76.5 72 68.5 73 61.5 74 47.0 75 40.5 76 31.0 77 21.5 78 15.5 79 13.5 80 8.5 81 4.0 82 6.0 83 6.0 84 3.5 85 2.0 86 1.0 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2742617262664096 2 0.08388040052891252 3 0.08805500379597646 4 0.10174381916006983 5 0.10417091408278141 6 0.0794145458711232 7 0.0893170931557865 8 0.08640457924853258 9 0.09067626631250497 10-11 0.08470561280263446 12-13 0.08941417695269495 14-15 0.09654983602546702 16-17 0.08625895355316988 18-19 0.0968410874161924 20-21 0.09795755108063975 22-23 0.11111240556173656 24-25 0.12378184105829104 26-27 0.12630601977791112 28-29 0.11839369032987132 30-31 0.1107240703741027 32-33 0.1053844615441372 34-35 0.3730930315192255 36-37 0.5714837705016708 38-39 0.5558047373009539 40-41 0.5214856150938121 42-43 0.5450284358441144 44-45 0.5654645750933461 46-47 0.554348480347327 48-49 0.5756098318702805 50-51 0.5678431281176034 52-53 0.5708527258217658 54-55 0.5615812232170075 56-57 0.5350288047625428 58-59 0.5307085758001161 60-61 0.48211813544743015 62-63 0.5518243016277069 64-65 0.5837163289121372 66-67 0.5957061778303325 68-69 0.5974536861746849 70-71 0.5926965801261701 72-73 0.5854152953580353 74-75 0.5833765356229575 76-77 0.5671635415392442 78-79 0.5449798939456603 80-81 0.5397373689126032 82-83 0.5546882736365066 84-85 0.5628433125768175 86-87 0.5532320166828797 88-89 0.5657558264840714 90-91 0.5793960999497105 92-93 0.588085099773018 94-95 0.5867744685147538 96-97 0.5843473735920423 98-99 0.5871142618039334 100-101 0.564396653327353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1030038.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.61112114600768 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18063225183522 14.550292181103803 2 8.87416876124768 3.3031645977139488 3 3.4465548732869395 1.9243275084931912 4 1.756335734718327 1.3074950852762075 5 1.1396679632038633 1.0605249264705459 6 0.7792916693826046 0.8702095000172531 7 0.6185248622660368 0.8058008800415646 8 0.4316969596511517 0.6427491531544618 9 0.37871569578620123 0.6343491324754422 >10 3.3306003679364675 13.239940782698124 >50 0.5908314381355566 7.699076999502987 >100 0.42531482957984545 14.62472717777415 >500 0.02199904290930235 2.7214212967386615 >1k 0.015189815342137336 6.514273673910558 >5k 0.004190293887486162 5.884341216285267 >10k+ 0.006285440831229243 24.21730588834386 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 55515 5.389606985373355 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 36466 3.540257738064033 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 25183 2.444861257545838 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17919 1.7396445568027588 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15895 1.5431469518600285 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15683 1.5225651869154342 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15565 1.5111092988802355 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14955 1.4518881827660728 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14868 1.4434418924350365 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13117 1.2734481640483166 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12738 1.2366534050200089 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10523 1.021612794867762 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9559 0.9280240146480033 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8842 0.8584149322646348 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8415 0.816960150984721 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7935 0.7703599284686584 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7587 0.7365747671445131 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6682 0.6487139309423535 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5723 0.555610569707137 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5620 0.5456109386255653 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4768 0.4628955436595543 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4490 0.4359062481190014 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3962 0.38464600335133264 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3722 0.3613458920933014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3695 0.3587246295767729 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3431 0.33309450719293854 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3286 0.31901735664121134 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3056 0.29668808335226465 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2978 0.2891155471934045 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2497 0.24241824088043354 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2465 0.23931155937936271 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2422 0.2351369561122988 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2417 0.23465153712775647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2238 0.21727353748114148 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2129 0.20669140361811894 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2027 0.19678885633345566 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1915 0.18591547107970774 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1737 0.16863455523000123 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1697 0.1647512033536627 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1339 0.12999520406043272 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1338 0.12989812026352426 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1317 0.12785936052844654 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1290 0.12523809801191801 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1183 0.1148501317427124 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1153 0.11193761783545851 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1113 0.10805426595911995 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1084 0.10523883584877451 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1043 0.10125840017552751 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1033 0.10028756220644287 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.708379690846357E-5 0.0 5 0.0 0.0 0.0 9.708379690846357E-5 0.0 6 0.0 0.0 0.0 9.708379690846357E-5 0.0 7 0.0 0.0 0.0 9.708379690846357E-5 0.0 8 0.0 0.0 0.0 1.9416759381692713E-4 0.0 9 0.0 0.0 0.0 2.912513907253907E-4 0.0 10-11 0.0 0.0 0.0 2.912513907253907E-4 0.0 12-13 0.0 0.0 0.0 2.912513907253907E-4 0.0 14-15 0.0 0.0 0.0 3.397932891796225E-4 0.0 16-17 0.0 0.0 0.0 5.339608829965496E-4 0.0 18-19 0.0 0.0 0.0 9.708379690846357E-4 0.0 20-21 0.0 0.0 0.0 0.0015047988520811855 0.0 22-23 0.0 0.0 0.0 0.0023785530242573577 0.0 24-25 0.0 0.0 0.0 0.003931893774792775 0.0 26-27 0.0 0.0 0.0 0.006310446799050132 0.0 28-29 0.0 0.0 0.0 0.018348837615699616 0.0 30-31 0.0 0.0 0.0 0.0458235521407948 0.0 32-33 0.0 0.0 0.0 0.10897656202975037 0.0 34-35 0.0 0.0 0.0 0.19601218595818795 0.0 36-37 0.0 0.0 0.0 0.3098429378333615 0.0 38-39 0.0 0.0 0.0 0.4790114539463593 0.0 40-41 0.0 0.0 0.0 0.6556554224213087 0.0 42-43 0.0 0.0 0.0 0.8540947033022083 0.0 44-45 0.0 0.0 0.0 1.0922412571186695 0.0 46-47 0.0 0.0 0.0 1.3880070444003036 0.0 48-49 0.0 0.0 0.0 1.6371726091658756 0.0 50-51 0.0 0.0 0.0 1.9210941732246773 0.0 52-53 0.0 0.0 0.0 2.195210273795724 0.0 54-55 0.0 0.0 0.0 2.505975507699716 0.0 56-57 0.0 0.0 0.0 2.959987883942146 0.0 58-59 0.0 0.0 0.0 3.346381395637831 0.0 60-61 0.0 0.0 0.0 3.7761713645515993 0.0 62-63 0.0 0.0 0.0 4.213970746710316 0.0 64-65 0.0 0.0 0.0 4.657546614785085 0.0 66-67 0.0 0.0 0.0 5.1888376933666525 0.0 68-69 0.0 0.0 0.0 5.802795625015776 0.0 70-71 0.0 0.0 0.0 6.4074335121616866 0.0 72-73 0.0 0.0 0.0 6.949015473215551 0.0 74-75 0.0 0.0 0.0 7.643018995415703 0.0 76-77 0.0 0.0 0.0 8.239064966535215 0.0 78-79 0.0 0.0 0.0 9.18786491372163 0.0 80-81 0.0 0.0 0.0 10.144771357949901 0.0 82-83 0.0 0.0 0.0 10.894452437677057 0.0 84-85 0.0 0.0 0.0 11.770488079080579 0.0 86-87 0.0 0.0 0.0 12.633757201190635 0.0 88-89 0.0 0.0 0.0 13.585712371776577 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 125 0.0 49.315254 1 CGCCGCA 25 0.0016036612 38.059868 92-93 GTAAGCG 255 0.0 34.51255 94-95 GCTTTGC 1180 0.0 31.746292 1 CATATAG 345 0.0 31.553974 3 ATACCTT 320 0.0 29.578976 6 CTTTGCG 1275 0.0 29.326635 2 TTAAGGG 65 0.0059464793 29.126745 3 CGTTTGC 50 4.9200702E-5 28.529573 54-55 TTTGCGT 1350 0.0 27.697376 3 TTGCGTT 1360 0.0 27.49372 4 CTCCCGC 190 4.129106E-10 27.399471 7 CCGGGTT 35 0.008345756 27.17434 52-53 GAGTGGC 35 0.0083467495 27.173676 82-83 GGCATAT 70 0.0084617315 27.096292 1 TACTCCC 195 5.5842975E-10 26.699516 5 GACATAT 400 0.0 26.080181 1 TTGCGTG 55 9.3979645E-5 25.942307 56-57 GCTTATG 170 1.14139766E-7 25.103918 1 CATGGGG 545 0.0 24.316824 4 >>END_MODULE