##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938954_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1030038 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02100505029912 33.0 31.0 34.0 30.0 34.0 2 32.2270945343764 34.0 31.0 34.0 30.0 34.0 3 32.3319120265466 34.0 31.0 34.0 30.0 34.0 4 35.85720623899312 37.0 35.0 37.0 35.0 37.0 5 35.79552113611342 37.0 35.0 37.0 35.0 37.0 6 35.9029550366103 37.0 35.0 37.0 35.0 37.0 7 35.86453703649768 37.0 35.0 37.0 35.0 37.0 8 35.8719192884146 37.0 35.0 37.0 35.0 37.0 9 37.456161811505986 39.0 37.0 39.0 34.0 39.0 10-11 37.36320698848004 39.0 37.0 39.0 34.0 39.0 12-13 37.35368889303113 39.0 37.0 39.0 34.0 39.0 14-15 38.35094773202542 40.0 38.0 41.0 33.5 41.0 16-17 38.439987650941035 40.0 38.0 41.0 33.5 41.0 18-19 38.687759577801984 40.0 38.0 41.0 34.0 41.0 20-21 38.68045742001752 40.0 38.0 41.0 34.0 41.0 22-23 38.63574110858046 40.0 38.0 41.0 34.0 41.0 24-25 38.48555684353393 40.0 38.0 41.0 34.0 41.0 26-27 38.249400507554085 40.0 37.5 41.0 33.5 41.0 28-29 38.17785363258443 40.0 37.5 41.0 33.5 41.0 30-31 38.163760463206216 40.0 37.5 41.0 33.5 41.0 32-33 37.96835068220784 40.0 37.0 41.0 33.0 41.0 34-35 37.82773111283273 40.0 37.0 41.0 33.0 41.0 36-37 37.66805884831433 40.0 37.0 41.0 32.5 41.0 38-39 37.51855028649429 40.0 37.0 41.0 32.5 41.0 40-41 37.621077571895405 40.0 37.0 41.0 33.0 41.0 42-43 37.44316860154674 40.0 37.0 41.0 32.0 41.0 44-45 37.21926763866964 40.0 37.0 41.0 31.0 41.0 46-47 36.95006834699302 40.0 36.5 41.0 31.0 41.0 48-49 36.61158617449065 39.0 36.0 41.0 30.0 41.0 50-51 36.37470607880486 39.0 36.0 41.0 30.0 41.0 52-53 36.35028513511152 39.0 36.0 41.0 29.5 41.0 54-55 36.22395532980337 39.0 36.0 40.5 29.0 41.0 56-57 35.855675227515874 39.0 35.0 40.0 28.0 41.0 58-59 35.603247647174186 39.0 35.0 40.0 28.0 41.0 60-61 34.94487145134451 38.0 34.0 40.0 26.0 41.0 62-63 34.79251930511302 38.0 34.0 40.0 26.5 41.0 64-65 34.59790124247843 37.5 34.0 39.5 26.0 41.0 66-67 33.995407450987244 37.0 33.5 39.0 26.0 40.0 68-69 33.46864824404537 36.0 33.0 39.0 25.5 40.0 70-71 32.788214609558096 35.5 32.0 37.5 24.0 39.5 72-73 32.16482644329626 35.0 31.5 37.0 22.5 39.0 74-75 31.76803816946559 35.0 31.0 37.0 22.0 39.0 76-77 29.765348462872243 33.0 28.5 34.5 20.0 36.5 78-79 30.762669435496555 34.0 30.5 36.0 20.0 37.0 80-81 30.666923938728473 34.0 31.0 35.0 20.0 37.0 82-83 30.29061646269361 34.0 30.0 35.0 18.0 36.5 84-85 29.843534898712477 34.0 30.0 35.0 14.0 36.0 86-87 29.502652814750526 34.0 29.5 35.0 7.0 36.0 88-89 28.86421520371093 34.0 29.0 35.0 2.0 35.0 90-91 28.330258204066258 33.0 28.5 35.0 2.0 35.0 92-93 28.041195082123185 33.0 28.0 35.0 2.0 35.0 94-95 27.58066789768921 33.0 27.0 35.0 2.0 35.0 96-97 26.825196740314432 32.5 25.0 34.0 2.0 35.0 98-99 26.004973117496636 32.0 24.5 34.0 2.0 35.0 100-101 21.60117636436714 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02378958139244247 1101 2 0.049097223065530216 1101 3 0.08862782145142489 1101 4 0.020419552234251626 1101 5 0.1316320221433429 1101 6 0.0321908909661488 1101 7 -0.030096344606128866 1101 8 0.15477445623508146 1101 9 -0.0385893440519709 1101 10-11 0.14364384063614466 1101 12-13 0.08068635921718936 1101 14-15 0.3326790328181133 1101 16-17 0.18190025266866172 1101 18-19 0.15486416208747755 1101 20-21 0.17979993674004646 1101 22-23 0.14759721110802104 1101 24-25 0.14148461226540832 1101 26-27 0.17357290539914771 1101 28-29 0.21747146929880046 1101 30-31 0.08102167211337274 1101 32-33 0.06521767802467338 1101 34-35 0.21328576762023488 1101 36-37 0.2664177876397318 1101 38-39 0.21796062595186072 1101 40-41 0.2768406074997287 1101 42-43 0.29639629797787137 1101 44-45 0.2741993191954535 1101 46-47 0.3982214995338893 1101 48-49 0.32048195657289824 1101 50-51 0.3822861136449234 1101 52-53 0.2682438106778804 1101 54-55 0.2688646845572009 1101 56-57 0.27290707343912146 1101 58-59 0.19447575789880744 1101 60-61 0.3957309660533781 1101 62-63 0.259085283942305 1101 64-65 0.1407870201374024 1101 66-67 0.3749701548434743 1101 68-69 0.5853156058679829 1101 70-71 0.5005776471077112 1101 72-73 0.42615069967381913 1101 74-75 0.06805709529786697 1101 76-77 -0.2651914134806148 1101 78-79 0.2580448221440257 1101 80-81 0.4394881506952615 1101 82-83 0.4660810436271028 1101 84-85 0.358222190882703 1101 86-87 0.5225310307341253 1101 88-89 0.5873201323995083 1101 90-91 0.49374288249834564 1101 92-93 0.5293233615088155 1101 94-95 0.4517432502075245 1101 96-97 0.36599716884288824 1101 98-99 -0.06782377039235499 1101 100-101 0.8447799119474553 1102 1 0.17343020907699724 1102 2 0.05911120660980629 1102 3 0.05916573451482776 1102 4 0.07548870634913385 1102 5 0.06764298944189306 1102 6 -0.004319045463610394 1102 7 0.05109650421887579 1102 8 0.1300145150327836 1102 9 0.15458639998712442 1102 10-11 0.08090461703307028 1102 12-13 0.24601015224163802 1102 14-15 0.15558376813300612 1102 16-17 0.09437901486250411 1102 18-19 0.08373660161938545 1102 20-21 0.14456725151774208 1102 22-23 0.14181647117331408 1102 24-25 0.19915659076988845 1102 26-27 0.05539942831688904 1102 28-29 0.14301148228010874 1102 30-31 0.24820675601464615 1102 32-33 0.16022166309616637 1102 34-35 0.08961492020790018 1102 36-37 0.10236706984959909 1102 38-39 0.1840105610453051 1102 40-41 0.20140940844660093 1102 42-43 0.11509344208950978 1102 44-45 0.09535312863744849 1102 46-47 0.02154037206886983 1102 48-49 -0.0754014884378833 1102 50-51 0.010227191857318019 1102 52-53 -0.08327707873279877 1102 54-55 0.16035261957428304 1102 56-57 -0.045735783021946474 1102 58-59 0.011924882614842147 1102 60-61 -0.33692442787213395 1102 62-63 -0.05202292387320284 1102 64-65 -0.014258509900976435 1102 66-67 0.0763487032274881 1102 68-69 0.1163961806727869 1102 70-71 0.13384965329293408 1102 72-73 0.10893960542568237 1102 74-75 0.2988655159686999 1102 76-77 -0.09234490316238464 1102 78-79 0.07787126870111649 1102 80-81 0.13872024889506207 1102 82-83 0.2510041102708591 1102 84-85 0.46916347908689104 1102 86-87 0.22091421325517402 1102 88-89 0.4877732851399017 1102 90-91 0.7429327437128599 1102 92-93 0.7673764989770788 1102 94-95 0.8295071478460692 1102 96-97 0.29530211987662014 1102 98-99 0.7572262219715817 1102 100-101 1.115908546237204 1103 1 0.05632494591910131 1103 2 0.058396718777324 1103 3 0.17688644758783312 1103 4 0.10452803855061177 1103 5 0.16664553613119892 1103 6 0.06387563478540415 1103 7 0.07043719465463738 1103 8 0.13686227960550212 1103 9 0.0485167904794821 1103 10-11 0.11359774097195441 1103 12-13 0.07891550028918459 1103 14-15 0.26887253440352055 1103 16-17 0.2583999543078974 1103 18-19 0.18213784666749433 1103 20-21 0.08033055089521923 1103 22-23 0.26641324536686284 1103 24-25 0.34554524385421104 1103 26-27 0.29339108083527776 1103 28-29 0.30848261980133174 1103 30-31 0.34112908199087855 1103 32-33 0.31251722053816167 1103 34-35 0.21315764799511072 1103 36-37 -0.006507434960809633 1103 38-39 0.1360231530090914 1103 40-41 0.20959418490387094 1103 42-43 0.06480340247434668 1103 44-45 0.12783756553614722 1103 46-47 0.2846063030252992 1103 48-49 0.07916132989827673 1103 50-51 -0.10312170457731185 1103 52-53 -8.103271764881015E-4 1103 54-55 0.10182828454315995 1103 56-57 0.03721200984733031 1103 58-59 -0.06461020510446502 1103 60-61 -0.05741820263160946 1103 62-63 0.0056348293809023176 1103 64-65 -0.06544696490946222 1103 66-67 0.1782374977945551 1103 68-69 0.1709837584882905 1103 70-71 0.08230041729972015 1103 72-73 0.027880607123478285 1103 74-75 0.3043904169307865 1103 76-77 0.1719059457340535 1103 78-79 0.043452055345145624 1103 80-81 0.24498793423745013 1103 82-83 0.0949062042153983 1103 84-85 0.020670270258371204 1103 86-87 -0.13172090276067294 1103 88-89 0.2100780389599386 1103 90-91 0.29284077993244395 1103 92-93 0.3634786495147111 1103 94-95 0.15485731762536048 1103 96-97 0.28569190482285833 1103 98-99 0.3776598665669404 1103 100-101 0.94025418856884 1104 1 -0.049990843554585496 1104 2 -0.024002828477911464 1104 3 0.015086787146401548 1104 4 0.032462390559665266 1104 5 0.10909035786294652 1104 6 0.0798914808521829 1104 7 0.0553947498329066 1104 8 0.04124258520822366 1104 9 -0.044975003521649626 1104 10-11 0.07548795036640854 1104 12-13 0.009102257504807199 1104 14-15 -0.024789038884534875 1104 16-17 -0.0657087044357425 1104 18-19 0.01891204021587356 1104 20-21 0.13748391818440808 1104 22-23 -0.003315107774056969 1104 24-25 0.07321360831374335 1104 26-27 0.13485118270285312 1104 28-29 -0.13010255280760674 1104 30-31 -0.1118590334590408 1104 32-33 -0.09883535444769365 1104 34-35 0.008913165821937241 1104 36-37 -0.08199696523811895 1104 38-39 0.03530442069443751 1104 40-41 0.010312917218527673 1104 42-43 -0.019950417559613243 1104 44-45 0.013949619865520901 1104 46-47 -0.1329771717907775 1104 48-49 -0.08562078668349926 1104 50-51 -0.06999210638829112 1104 52-53 -0.1388691839959577 1104 54-55 0.020045602030435816 1104 56-57 -0.05413490582896685 1104 58-59 -0.001978626157089991 1104 60-61 0.018908905461202608 1104 62-63 0.21444637437240743 1104 64-65 0.23784675325692461 1104 66-67 0.173410106736263 1104 68-69 0.30558477716401455 1104 70-71 0.17819175855608904 1104 72-73 -0.04682793843396382 1104 74-75 0.02040060368799601 1104 76-77 -0.12289314877641999 1104 78-79 0.12276161957830922 1104 80-81 0.25968516117576357 1104 82-83 0.25881112781472027 1104 84-85 0.03385646154303146 1104 86-87 -0.03967789200798322 1104 88-89 -0.04841092538641689 1104 90-91 0.05771344546724677 1104 92-93 0.04733263932795495 1104 94-95 0.17014311275835325 1104 96-97 0.25339422513977894 1104 98-99 0.34957271319965244 1104 100-101 1.2801410023775581 1105 1 -0.1185468489526933 1105 2 -0.06788946815402142 1105 3 -0.07449485927735111 1105 4 -0.00705177948082536 1105 5 0.03405661967131124 1105 6 0.03770524603437053 1105 7 -0.06330970360838961 1105 8 -0.03738089657315413 1105 9 -0.11183060406145984 1105 10-11 -0.1478993640735382 1105 12-13 -0.0569571217124718 1105 14-15 -0.08312911985619564 1105 16-17 -0.06419723345057804 1105 18-19 -0.020575139271301168 1105 20-21 0.031141138157416037 1105 22-23 0.013931855788698044 1105 24-25 -0.017258726369114186 1105 26-27 -0.11186946507042705 1105 28-29 -0.020210515020835373 1105 30-31 -0.08747306854676395 1105 32-33 -0.022114706674415174 1105 34-35 -0.11101935779480243 1105 36-37 -0.09879858467131442 1105 38-39 0.06637054755002936 1105 40-41 0.13719550831164185 1105 42-43 0.07309951496400657 1105 44-45 0.01405758207874186 1105 46-47 0.04823740310801128 1105 48-49 -0.16529690004382047 1105 50-51 -0.2227991239321554 1105 52-53 -0.1336060261012264 1105 54-55 -0.2042593912219317 1105 56-57 -0.21548443349427515 1105 58-59 -0.17999482048907822 1105 60-61 -0.3991882605307069 1105 62-63 -0.3713296040351466 1105 64-65 -0.35335432636521347 1105 66-67 -0.08324306465375031 1105 68-69 -0.19085246979955173 1105 70-71 0.016626306464324614 1105 72-73 -0.1288522299319368 1105 74-75 -0.2396128915886564 1105 76-77 -0.3709228383850558 1105 78-79 -0.04908723332317422 1105 80-81 -0.1611245554234273 1105 82-83 -0.14704582225275686 1105 84-85 -0.29196000269448774 1105 86-87 -0.18301122534131764 1105 88-89 -0.05545545979936861 1105 90-91 0.09570264924592564 1105 92-93 0.21872265282323156 1105 94-95 -0.14329818278820738 1105 96-97 -0.13780685448235275 1105 98-99 -0.1925055594049354 1105 100-101 0.1476983573033337 1106 1 0.062431052025203826 1106 2 -0.03536155562611043 1106 3 -0.020259085041573144 1106 4 0.001279628850582526 1106 5 0.04864043425512676 1106 6 0.09765134598046643 1106 7 0.10391906362038128 1106 8 0.13096283177057444 1106 9 -0.026877959108816185 1106 10-11 0.16894825277407222 1106 12-13 0.23290658815123777 1106 14-15 0.14146932474224627 1106 16-17 0.17890432965097602 1106 18-19 0.18982295112679282 1106 20-21 0.13297835999463814 1106 22-23 0.22571286756971176 1106 24-25 0.19850626939588523 1106 26-27 0.23126443806347652 1106 28-29 0.3507619959516788 1106 30-31 0.354248653174956 1106 32-33 0.4412378766781657 1106 34-35 0.48041361363817714 1106 36-37 0.38083797496523886 1106 38-39 0.4664229076023929 1106 40-41 0.5659812170973524 1106 42-43 0.6321400940045834 1106 44-45 0.4719138999350676 1106 46-47 0.698615473486079 1106 48-49 0.5352190004720754 1106 50-51 0.457921596788573 1106 52-53 0.7135411210459282 1106 54-55 0.7432019162393715 1106 56-57 0.7293896713798276 1106 58-59 0.9409954005011443 1106 60-61 0.638765077422633 1106 62-63 0.8413754086698688 1106 64-65 0.7288925358816485 1106 66-67 1.0085210071103177 1106 68-69 0.9510186320715519 1106 70-71 0.7522542420922562 1106 72-73 0.8673378062580994 1106 74-75 0.8851195331437651 1106 76-77 0.4968018093395905 1106 78-79 0.8208165165805745 1106 80-81 0.9738119195130466 1106 82-83 1.016776461569524 1106 84-85 0.9944618037273223 1106 86-87 0.9889207065906156 1106 88-89 1.0258212014772923 1106 90-91 1.336029129572406 1106 92-93 1.1017733958739733 1106 94-95 1.185311965821942 1106 96-97 1.3883639316884313 1106 98-99 1.2128644259650478 1106 100-101 1.1798227894277638 1107 1 -0.06585247343573997 1107 2 0.02163596608914986 1107 3 0.015394512103959812 1107 4 -0.011735402309604126 1107 5 -0.04504801225589716 1107 6 -0.03222651575223523 1107 7 0.052493646267542715 1107 8 0.04956389250872917 1107 9 0.15685012381281638 1107 10-11 -0.019097151501171084 1107 12-13 0.0013953389988614617 1107 14-15 -0.044870745166370796 1107 16-17 0.06313785753369672 1107 18-19 0.04352897569975056 1107 20-21 -0.10186030931983936 1107 22-23 0.04705840665718597 1107 24-25 0.08459094277894508 1107 26-27 -0.06900811271310658 1107 28-29 0.009557888618537902 1107 30-31 0.00282263037797037 1107 32-33 0.14413684419407247 1107 34-35 0.06920603509188084 1107 36-37 0.11086487686714719 1107 38-39 0.09976643257898132 1107 40-41 0.10502429243074829 1107 42-43 0.10643646699047338 1107 44-45 0.023603694568414824 1107 46-47 -0.08105229912523981 1107 48-49 -0.16492469160709788 1107 50-51 -0.07966740350204304 1107 52-53 0.056817360995552235 1107 54-55 -0.07700236061812404 1107 56-57 0.14531012812689426 1107 58-59 -0.12428762445929209 1107 60-61 -0.12079185854558716 1107 62-63 -0.10837726739329412 1107 64-65 -0.039035674586884284 1107 66-67 0.0887549709128308 1107 68-69 0.14295956493990758 1107 70-71 0.022219772138434735 1107 72-73 0.03799485443530415 1107 74-75 -0.08189990564986616 1107 76-77 -0.40494217254551756 1107 78-79 0.10370071125912617 1107 80-81 0.3714328267105671 1107 82-83 0.3325070106670118 1107 84-85 0.12413129176374582 1107 86-87 0.34092694670169266 1107 88-89 0.5311465873470347 1107 90-91 0.5604819089647002 1107 92-93 0.7445270366114869 1107 94-95 0.6945414574103062 1107 96-97 0.5745359908443639 1107 98-99 0.3271894364594168 1107 100-101 1.0493939735150768 1108 1 -0.03177029217614091 1108 2 -0.07502622029077344 1108 3 0.011442259159771595 1108 4 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-1.3353481435399956 2316 52-53 -1.3598805359051411 2316 54-55 -1.366280507360699 2316 56-57 -1.3561782494822125 2316 58-59 -1.3293160874574 2316 60-61 -1.075900176066142 2316 62-63 -1.2880716854831107 2316 64-65 -1.252720842202315 2316 66-67 -1.4839067147291658 2316 68-69 -1.5162521681404257 2316 70-71 -1.3601157749836439 2316 72-73 -1.2402851107765827 2316 74-75 -1.6926747317093245 2316 76-77 -2.0160510435132615 2316 78-79 -1.8318926631874284 2316 80-81 -1.882089864624028 2316 82-83 -2.15117855226784 2316 84-85 -2.349456231955422 2316 86-87 -2.6190896567138644 2316 88-89 -3.0176575714132454 2316 90-91 -3.205413489517273 2316 92-93 -3.778652912787628 2316 94-95 -3.848577217479008 2316 96-97 -4.142663566397889 2316 98-99 -4.673440702693018 2316 100-101 -4.6721809790465905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 20.0 6 56.0 7 115.0 8 282.0 9 462.0 10 827.0 11 1261.0 12 1960.0 13 2696.0 14 3258.0 15 4524.0 16 5772.0 17 8496.0 18 8397.0 19 5755.0 20 5498.0 21 5798.0 22 6461.0 23 7179.0 24 8365.0 25 9981.0 26 11794.0 27 14639.0 28 17778.0 29 21980.0 30 26842.0 31 33029.0 32 40567.0 33 51452.0 34 68566.0 35 97670.0 36 152288.0 37 229085.0 38 165522.0 39 11657.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.611591569873696 21.676298042762728 15.735969364134245 16.97614102322934 2 14.244037598612866 27.59597218743386 33.13935990711022 25.02063030684305 3 26.66464732369097 25.31236711655298 32.46686044592529 15.55612511383075 4 11.0268747366602 17.962638271597747 44.300792786285555 26.709694205456497 5 9.539218199046243 42.83235860633456 35.90160305699241 11.726820137626792 6 24.263862109941574 40.84723087886078 15.874851219081238 19.01405579211641 7 22.163162912436242 35.64810230302183 23.935233457406426 18.253501327135506 8 23.290111626949685 27.030556154238973 21.011554913508046 28.6677773053033 9 19.42530275582066 23.59738184416497 28.286917570031395 28.69039782998297 10-11 24.638168688922157 24.755688625079852 27.971977732860342 22.63416495313765 12-13 30.5960556794992 24.764814501989246 25.99326432617049 18.64586549234106 14-15 22.961320785399554 27.977080134579452 31.87563196373675 17.18596711628424 16-17 17.511083624875667 30.622843165191032 31.339340715458725 20.52673249447457 18-19 17.030245486088862 35.05686197984929 28.968154572938086 18.944737961123764 20-21 21.16829670361676 27.667328778161583 26.490915869123278 24.673458649098382 22-23 18.83867391300127 27.885427527916445 29.684050491341097 23.59184806774119 24-25 24.7115154974865 28.697776198548013 27.159920313619494 19.430787990345987 26-27 21.781245828428577 24.27091246677912 35.54399718456852 18.403844520223778 28-29 17.263003889176904 34.157963104273826 29.653663262908747 18.925369743640527 30-31 19.789561161821215 27.620728555645517 28.53792772693823 24.051782555595036 32-33 20.729332315895142 28.50035629753465 30.848570635258117 19.921740751312086 34-35 24.57006464503483 26.3871352642864 29.0954112559156 19.947388834763174 36-37 21.634032141790883 25.067344357953043 34.549828028554295 18.748795471701776 38-39 19.8803767356054 29.41382658510535 31.906544324691716 18.799252354597535 40-41 19.21838698810013 30.649332871202343 30.280259142863525 19.852020997834003 42-43 18.23983511135857 30.680413211867442 26.229128611489877 24.85062306528412 44-45 21.142526501578104 25.648158845396214 28.398556162763715 24.810758490261964 46-47 24.58852066461916 29.73966380705094 28.8967300578088 16.775085470521102 48-49 21.856604110631032 28.080946646065453 29.86311650121137 20.19933274209215 50-51 15.16403608624 29.788477548038532 35.248111103290434 19.79937526243104 52-53 19.829122809061413 32.75874288133059 26.328883012083047 21.083251297524946 54-55 17.601292379504446 27.237004848364816 29.895401917210823 25.266300854919916 56-57 22.597127484617072 26.191897774645206 28.357012071399307 22.85396266933841 58-59 26.584886234393117 25.997575809353958 27.654898937851115 19.762639018401813 60-61 20.72176068364451 28.879456393099478 32.99223599017096 17.40654693308505 62-63 18.2833060527864 29.180088501589264 31.82435987798509 20.71224556763925 64-65 17.712821961615813 33.76895023317203 28.334224081061443 20.18400372415072 66-67 20.656833500401692 27.758363328778398 25.809318773710153 25.77548439710976 68-69 18.28217815293788 28.262717356321893 29.239980408137907 24.21512408260232 70-71 23.908729580850416 28.4917158396098 26.500964042103302 21.098590537436483 72-73 21.149413905118063 24.422836827379186 33.56774216096882 20.860007106533935 74-75 17.508237560167682 34.30781194480203 27.516169306375104 20.667781188655177 76-77 19.851920542160943 27.495499488710813 27.542983284926102 25.109596684202135 78-79 20.373238838391806 27.840539786172837 29.846971590840138 21.939249784595223 80-81 23.354478184299996 27.31418646690705 27.61354435467429 21.717790994118662 82-83 20.398033858945013 26.268933767492076 32.47525819435788 20.857774179205038 84-85 19.20633025189362 29.0262592253878 29.929866665113323 21.837543857605255 86-87 19.067592064600905 30.380868627619694 28.47543169599735 22.076107611782053 88-89 18.87214826904721 29.436434881718792 25.654926782944266 26.036490066289737 90-91 20.676858340922706 25.40118150693271 28.036536129469003 25.885424022675586 92-93 23.11099202165357 29.316879571433287 28.482104543715863 19.09002386319728 94-95 20.88160825134607 27.4226290680538 29.087955978323134 22.607806702277003 96-97 15.805147706647807 28.594076977257494 32.8081228583786 22.7926524577161 98-99 19.731505051269956 30.376743382282985 25.804387799285074 24.08736376716199 100-101 18.48077977932789 26.44458683442796 28.637424941870908 26.43720844437325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2288.0 1 1351.5 2 405.5 3 365.0 4 311.0 5 341.0 6 564.0 7 1061.5 8 1592.0 9 1788.0 10 1522.5 11 1156.5 12 1020.5 13 987.0 14 966.5 15 954.0 16 1056.0 17 1188.0 18 1228.0 19 1373.0 20 1609.0 21 1884.5 22 2202.5 23 2449.5 24 2735.0 25 3634.0 26 5387.5 27 6980.0 28 7388.5 29 8128.5 30 10402.0 31 12766.5 32 16324.0 33 21658.0 34 27160.0 35 30279.0 36 34203.5 37 38661.0 38 37718.0 39 35094.0 40 32090.5 41 28483.0 42 25441.5 43 23863.5 44 23273.0 45 23354.0 46 29222.0 47 76474.5 48 163893.0 49 151624.5 50 65528.5 51 26879.5 52 12263.0 53 5582.0 54 2858.5 55 2130.0 56 1771.5 57 1472.0 58 1305.0 59 1118.0 60 957.0 61 809.0 62 634.5 63 500.0 64 372.0 65 286.0 66 221.5 67 169.0 68 114.5 69 71.5 70 57.5 71 43.5 72 37.0 73 32.0 74 20.5 75 14.5 76 8.0 77 5.0 78 5.0 79 4.0 80 2.5 81 1.0 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3932864612761859 2 0.0 3 0.0 4 0.0 5 5.825027814507814E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014077150551727218 16-17 0.0026698044149827483 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.854189845423179E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002766888211891212 36-37 0.0068444076820466816 38-39 0.013397563973367973 40-41 0.002621262516528516 42-43 9.222960706304039E-4 44-45 4.854189845423179E-4 46-47 4.368770860880861E-4 48-49 1.4562569536269535E-4 50-51 5.339608829965497E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.397932891796225E-4 60-61 0.0028154301103454436 62-63 0.0 64-65 4.368770860880861E-4 66-67 0.001990217836623503 68-69 0.001796050242806576 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12290808688611489 78-79 4.854189845423179E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14635382383950882 88-89 0.4261007846312466 90-91 0.32843448494133226 92-93 0.0 94-95 0.0 96-97 6.310446799050132E-4 98-99 0.0 100-101 2.912513907253907E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1030038.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.64498426510907 #Duplication Level Percentage of deduplicated Percentage of total 1 75.91267806573909 12.635653319265176 2 9.82653420121284 3.2712501431948775 3 3.674827974359402 1.8350236143058463 4 1.9711909546275233 1.312417696932018 5 1.2430017123006574 1.0344871971374037 6 0.8574779303325099 0.8563623994837751 7 0.6546885939378114 0.7628096941248877 8 0.5169749482962479 0.6884031903877297 9 0.4175053790127994 0.6254433417839801 >10 3.70277470827898 13.143110440856995 >50 0.6853782547424323 7.995597281968327 >100 0.4832121199249714 15.192918612854134 >500 0.023956102680681775 2.7084779436765425 >1k 0.017528855620011056 6.50709662025805 >5k 0.005258656686003316 6.421803246829764 >10k+ 0.0070115422480044225 25.009145256940485 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 59671 5.79308724532493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34892 3.387447841730111 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23882 2.318555237767927 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17914 1.7391591378182163 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17388 1.6880930606443645 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 17169 1.666831709121411 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 16395 1.59168885031426 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 16208 1.5735341802923777 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 15068 1.462858651816729 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13987 1.3579110673586798 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13262 1.2875253146000438 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 11312 1.09821191062854 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 9251 0.8981222052001966 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8965 0.8703562392843759 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8537 0.8288043742075535 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 8288 0.8046305087773461 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7412 0.719585102685532 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6677 0.6482285119578113 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6003 0.5827940328415068 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5771 0.5602705919587433 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5126 0.4976515429527843 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4809 0.46687597933280134 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3883 0.37697638339556405 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3703 0.3595012999520406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3697 0.35891879717058983 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3477 0.33756036185072785 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3369 0.3270753117846138 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3125 0.30338686533894865 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3063 0.2973676699306239 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2902 0.2817371786283613 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2850 0.2766888211891212 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2619 0.2542624641032661 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2492 0.24193282189589124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2339 0.22707900096889633 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2259 0.2193122972162192 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2214 0.21494352635533834 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1979 0.19212883408184941 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1815 0.17620709138886137 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1777 0.17251790710633977 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1511 0.14669361712868845 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1467 0.14242193006471607 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1329 0.12902436609134807 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1302 0.12640310357481957 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1293 0.1255293494026434 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1207 0.11718014286851554 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1168 0.11339387478908544 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1139 0.11057844467874 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 1051 0.10203507055079522 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1033 0.10028756220644287 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1031 0.10009339461262594 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.708379690846357E-5 0.0 9 0.0 0.0 0.0 1.9416759381692713E-4 0.0 10-11 0.0 0.0 0.0 1.9416759381692713E-4 0.0 12-13 0.0 0.0 0.0 1.9416759381692713E-4 0.0 14-15 0.0 0.0 0.0 2.4270949227115892E-4 0.0 16-17 0.0 0.0 0.0 4.3687708608808605E-4 0.0 18-19 0.0 0.0 0.0 8.737541721761722E-4 0.0 20-21 0.0 0.0 0.0 0.0014562569536269534 0.0 22-23 0.0 0.0 0.0 0.0024270949227115893 0.0 24-25 0.0 0.0 0.0 0.004028977571701238 0.0 26-27 0.0 0.0 0.0 0.006407530595958596 0.0 28-29 0.0 0.0 0.0 0.018397379514153846 0.0 30-31 0.0 0.0 0.0 0.04630897112533712 0.0 32-33 0.0 0.0 0.0 0.11028719328801462 0.0 34-35 0.0 0.0 0.0 0.1983907389824453 0.0 36-37 0.0 0.0 0.0 0.31440587628805927 0.0 38-39 0.0 0.0 0.0 0.4865354482067652 0.0 40-41 0.0 0.0 0.0 0.6669171428626905 0.0 42-43 0.0 0.0 0.0 0.8678320605647558 0.0 44-45 0.0 0.0 0.0 1.1093280053745591 0.0 46-47 0.0 0.0 0.0 1.408346099852627 0.0 48-49 0.0 0.0 0.0 1.6587252120795544 0.0 50-51 0.0 0.0 0.0 1.944734077771888 0.0 52-53 0.0 0.0 0.0 2.220209351499653 0.0 54-55 0.0 0.0 0.0 2.5327220937479975 0.0 56-57 0.0 0.0 0.0 2.9902780285775865 0.0 58-59 0.0 0.0 0.0 3.3797782217743424 0.0 60-61 0.0 0.0 0.0 3.8121409113061846 0.0 62-63 0.0 0.0 0.0 4.252658639778339 0.0 64-65 0.0 0.0 0.0 4.697884932400552 0.0 66-67 0.0 0.0 0.0 5.230923519326471 0.0 68-69 0.0 0.0 0.0 5.848133758172029 0.0 70-71 0.0 0.0 0.0 6.457480209468001 0.0 72-73 0.0 0.0 0.0 7.003673650875016 0.0 74-75 0.0 0.0 0.0 7.705298251132483 0.0 76-77 0.0 0.0 0.0 8.308140088035586 0.0 78-79 0.0 0.0 0.0 9.26310485632569 0.0 80-81 0.0 0.0 0.0 10.221807350796766 0.0 82-83 0.0 0.0 0.0 10.974837821517264 0.0 84-85 0.0 0.0 0.0 11.855193691883212 0.0 86-87 0.0 0.0 0.0 12.719093858673176 0.0 88-89 0.0 0.0 0.0 13.672505286212742 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGAT 25 0.004963588 57.16621 1 GTATGGT 130 0.0 47.63851 1 GTAAGCG 310 0.0 38.272686 94-95 GCTTATG 180 0.0 37.052174 1 CTCCGAC 120 0.0 35.593597 94-95 CATATAG 370 0.0 32.066307 3 GGTATGC 60 0.003967855 31.638754 8 GACGCAG 80 4.4173375E-4 29.774069 1 TACAGTG 130 3.0598858E-7 29.205004 5 CTTTGCG 1065 0.0 28.96506 2 GCTTTGC 1090 0.0 28.845339 1 GCAGAGA 1505 0.0 28.06494 94-95 CCCTATA 395 0.0 27.633848 2 GACATAT 435 0.0 27.378452 1 GGACAAT 330 0.0 27.324377 6 AGAGTGC 70 0.008427342 27.118933 6 ATGCCGC 35 0.008429265 27.118933 94-95 ACATATA 455 0.0 27.118933 2 TGTACGT 70 0.008427342 27.118933 2 CGAGACA 45 8.9961605E-4 26.365627 94-95 >>END_MODULE