FastQCFastQC Report
Fri 27 May 2016
SRR938953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629697
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82770.3147510910952859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67310.25596104798385516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43340.16480986212479992No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36960.14054851186277353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104000.028.4770571
GTATCAA143450.025.3107321
GAGTACT77850.022.19614612-13
GTACATG97700.021.4719521
TACATGG96300.021.443812
ATCAACG167950.021.3762233
TATCAAC169150.021.2805752
CAACGCA170500.021.1120765
TCAACGC170200.021.0936344
AACGCAG175000.020.586076
GTACTTT83250.020.5579514-15
ACATGGG96900.020.3823383
AGAGTAC139500.018.6646310-11
AGTACTT83900.018.4231812-13
ACGCAGA193700.018.3542737
CGCAGAG192700.018.3512528
CATGGGG51700.017.8642924
ACTTTTT101050.016.96169916-17
GCAGAGT203850.016.5114699
GCGTAGA5750.016.5084381
Produced by FastQC (version 0.11.5)