##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2629697 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483593737225238 33.0 31.0 34.0 30.0 34.0 2 31.582540878283694 33.0 31.0 34.0 28.0 34.0 3 31.61592685393032 33.0 31.0 34.0 28.0 34.0 4 35.15751282372076 37.0 35.0 37.0 32.0 37.0 5 35.18812395496516 37.0 35.0 37.0 33.0 37.0 6 35.25801299541354 37.0 35.0 37.0 32.0 37.0 7 35.16457979759645 37.0 35.0 37.0 32.0 37.0 8 35.14353060447649 37.0 35.0 37.0 32.0 37.0 9 36.70002513597574 39.0 37.0 39.0 32.0 39.0 10-11 36.56936692706422 39.0 37.0 39.0 32.0 39.0 12-13 36.43675297952578 39.0 37.0 39.0 32.0 39.0 14-15 37.58449813799841 40.0 37.0 41.0 32.0 41.0 16-17 37.482222666717874 40.0 37.0 41.0 32.0 41.0 18-19 37.38252715046639 40.0 37.0 41.0 32.0 41.0 20-21 37.48131077458734 40.0 37.0 41.0 32.0 41.0 22-23 37.40425950214036 40.0 37.0 41.0 31.5 41.0 24-25 37.31384984657928 40.0 37.0 41.0 31.0 41.0 26-27 37.18279710552204 40.0 37.0 41.0 31.0 41.0 28-29 37.03780967921399 40.0 37.0 41.0 30.5 41.0 30-31 36.82716678005109 40.0 36.0 41.0 30.0 41.0 32-33 36.615796420652266 39.0 36.0 41.0 30.0 41.0 34-35 36.28066731642467 39.0 35.5 41.0 29.5 41.0 36-37 36.00698863785448 39.0 35.0 41.0 28.5 41.0 38-39 35.752201109101165 39.0 35.0 40.5 27.0 41.0 40-41 35.584473420321814 39.0 35.0 40.0 27.0 41.0 42-43 35.35831941854898 38.5 35.0 40.0 26.0 41.0 44-45 35.29608335104767 39.0 35.0 40.5 25.5 41.0 46-47 35.08617779919132 39.0 35.0 41.0 24.5 41.0 48-49 34.842013547568406 39.0 34.0 40.5 24.0 41.0 50-51 33.74752794713611 38.0 33.0 39.5 22.5 40.5 52-53 33.85873106293235 38.0 33.5 39.5 23.0 40.5 54-55 34.339986888223244 38.0 34.0 40.0 23.0 41.0 56-57 34.226915116076114 38.0 34.0 40.0 22.0 41.0 58-59 33.995805600417086 38.0 34.0 40.0 21.5 41.0 60-61 33.667131612501365 38.0 33.0 40.0 20.0 41.0 62-63 33.26668547745234 37.0 33.0 40.0 18.5 41.0 64-65 32.85913719337247 36.5 32.0 39.5 16.5 41.0 66-67 32.43389865828649 36.0 32.0 39.0 13.0 41.0 68-69 31.975668489563628 35.5 31.0 39.0 9.5 40.5 70-71 31.519234915657584 35.0 31.0 38.5 7.5 40.0 72-73 31.068896910936886 35.0 30.5 37.5 6.5 40.0 74-75 30.58302192229751 35.0 30.0 37.0 2.0 39.0 76-77 30.13826345772916 34.5 30.0 36.5 2.0 39.0 78-79 29.72168067271629 34.0 29.0 36.0 2.0 38.5 80-81 29.25028586943667 34.0 29.0 35.5 2.0 37.0 82-83 28.762821344055986 34.0 29.0 35.0 2.0 37.0 84-85 28.32619746685645 34.0 27.5 35.0 2.0 36.5 86-87 27.849109802384078 34.0 26.5 35.0 2.0 36.0 88-89 27.33781420445017 33.5 26.0 35.0 2.0 36.0 90-91 26.851537458498072 33.0 25.0 35.0 2.0 35.0 92-93 26.30144195319841 33.0 24.5 35.0 2.0 35.0 94-95 25.711913387740108 32.0 22.0 35.0 2.0 35.0 96-97 25.02077748881335 32.0 18.5 34.5 2.0 35.0 98-99 24.095023875374235 32.0 2.0 34.0 2.0 35.0 100-101 21.03705693089356 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32155978995320567 1101 2 0.43827187164599124 1101 3 0.5234376819303925 1101 4 0.5408589260419845 1101 5 0.32181999137944217 1101 6 0.41191814194856136 1101 7 0.3913548803179978 1101 8 0.3717028566145686 1101 9 0.5298795797079592 1101 10-11 0.4554231885619515 1101 12-13 0.3442028952563163 1101 14-15 0.6272446585440221 1101 16-17 0.7039069724996025 1101 18-19 0.700027788477307 1101 20-21 0.6022098602219756 1101 22-23 0.7279031441404129 1101 24-25 0.6783536200006068 1101 26-27 0.6874250670141819 1101 28-29 0.6194415159006681 1101 30-31 0.6254105072331413 1101 32-33 0.6029915808129047 1101 34-35 0.6971819994070003 1101 36-37 0.5563011827046296 1101 38-39 0.8205316088447248 1101 40-41 0.7811060461938766 1101 42-43 0.9023109460760708 1101 44-45 0.8868426361100319 1101 46-47 0.8879351503905397 1101 48-49 0.7729039362515877 1101 50-51 0.793088863228121 1101 52-53 0.8085852506938664 1101 54-55 0.8683679794324135 1101 56-57 0.8660322309961401 1101 58-59 0.9599400098286353 1101 60-61 0.9615506609153925 1101 62-63 0.8955312762131129 1101 64-65 0.5724009170573865 1101 66-67 0.6231185123263714 1101 68-69 0.714692367828583 1101 70-71 0.6659232659195204 1101 72-73 0.5913093408176593 1101 74-75 0.2922322711605112 1101 76-77 0.3378618463893126 1101 78-79 0.30120055744713525 1101 80-81 0.2698247580239084 1101 82-83 0.2435547108416536 1101 84-85 0.1425106196063446 1101 86-87 0.14532343642892087 1101 88-89 0.008873272485228512 1101 90-91 0.011469457827740115 1101 92-93 0.06496074951754593 1101 94-95 -0.045917789017089206 1101 96-97 -0.15188137678896396 1101 98-99 -0.17336396484305894 1101 100-101 0.27443603205411904 1102 1 0.31810839558988135 1102 2 0.34187059219575744 1102 3 0.4302553696095508 1102 4 0.3414386936014111 1102 5 0.37257883128410185 1102 6 0.4175794291085708 1102 7 0.5046266421097627 1102 8 0.47132310319418735 1102 9 0.630259466515561 1102 10-11 0.6511520963740409 1102 12-13 0.556363774918367 1102 14-15 0.685017665505498 1102 16-17 0.7014969988702546 1102 18-19 0.7667155636718164 1102 20-21 0.6705026171624411 1102 22-23 0.7465164972684164 1102 24-25 0.7934729615479199 1102 26-27 0.7613002459197915 1102 28-29 0.7561467448131509 1102 30-31 0.8344411222682808 1102 32-33 0.751458728207119 1102 34-35 0.7335121767986692 1102 36-37 0.6397922464277102 1102 38-39 0.8283384294669176 1102 40-41 0.6778625024688765 1102 42-43 0.5874257324497663 1102 44-45 0.8990442322215273 1102 46-47 0.8944721246614833 1102 48-49 0.9009463320461606 1102 50-51 0.886613647240857 1102 52-53 0.81091986054291 1102 54-55 0.9593729120918795 1102 56-57 0.8374616884969726 1102 58-59 0.9266338874885491 1102 60-61 0.9654449008549975 1102 62-63 0.940274852617982 1102 64-65 0.8285542363317049 1102 66-67 0.8813920214907398 1102 68-69 0.9090522225432167 1102 70-71 0.9196966236893935 1102 72-73 0.8502606238026544 1102 74-75 0.8585654030791439 1102 76-77 0.9807927571439237 1102 78-79 0.9015307575146316 1102 80-81 0.8345313995070676 1102 82-83 0.7419150484180328 1102 84-85 0.5978729193087595 1102 86-87 0.5496517850817746 1102 88-89 0.5727035599213508 1102 90-91 0.6039208081732781 1102 92-93 0.6425934464604417 1102 94-95 0.40211088123235683 1102 96-97 0.3108010469102105 1102 98-99 0.015445981041747103 1102 100-101 0.06433815251078556 1103 1 0.30026823082890175 1103 2 0.24771240085749824 1103 3 0.42638852689581697 1103 4 0.43986692047693765 1103 5 0.25955088464000653 1103 6 0.39278679606238853 1103 7 0.34818020979499664 1103 8 0.23419604827684282 1103 9 0.5247860452845359 1103 10-11 0.49436746227608097 1103 12-13 0.5944234388459648 1103 14-15 0.651367954131949 1103 16-17 0.6260508457271854 1103 18-19 0.6860936399833832 1103 20-21 0.721309300857591 1103 22-23 0.717907689682626 1103 24-25 0.7448529572891402 1103 26-27 0.8035739785779512 1103 28-29 0.7714353696093639 1103 30-31 0.7669927582707672 1103 32-33 0.6395520218934294 1103 34-35 0.6258338620817483 1103 36-37 0.5941345917415859 1103 38-39 0.8019288027787681 1103 40-41 0.6952013669123076 1103 42-43 0.6199102414006461 1103 44-45 0.7796311849348783 1103 46-47 0.7057819400140701 1103 48-49 0.7149873441247223 1103 50-51 0.5926893077402511 1103 52-53 0.5898284548256214 1103 54-55 0.802522541833369 1103 56-57 0.7220905043817396 1103 58-59 0.613171833811414 1103 60-61 0.7003543952429183 1103 62-63 0.7223338979302341 1103 64-65 0.6945749996858339 1103 66-67 0.640170696614021 1103 68-69 0.5136279395061578 1103 70-71 0.5530389481398963 1103 72-73 0.36885625766316466 1103 74-75 0.3303584636587402 1103 76-77 0.28628897905330675 1103 78-79 0.29334095917065284 1103 80-81 0.34491340773518075 1103 82-83 0.46850784221147634 1103 84-85 0.419402306684173 1103 86-87 0.3159967419591858 1103 88-89 0.21445522824549457 1103 90-91 0.4180068586288428 1103 92-93 0.3860570864157076 1103 94-95 0.3165511287182383 1103 96-97 0.050503237265573375 1103 98-99 0.1448308064865742 1103 100-101 0.1419344742000419 1104 1 0.3341517778427061 1104 2 0.3624309857618293 1104 3 0.28184125695856466 1104 4 0.39675777535951084 1104 5 0.3950691667743129 1104 6 0.3727072926944359 1104 7 0.43029526437197774 1104 8 0.3887292849284094 1104 9 0.42371720019541925 1104 10-11 0.5979509186753305 1104 12-13 0.48104127925481066 1104 14-15 0.5173717210475672 1104 16-17 0.5141540039871657 1104 18-19 0.5276775548667061 1104 20-21 0.5926083456168527 1104 22-23 0.5811325118396695 1104 24-25 0.6028127556479816 1104 26-27 0.495005754147698 1104 28-29 0.47400719304117445 1104 30-31 0.5494895385516259 1104 32-33 0.5640289056709946 1104 34-35 0.6936809776495778 1104 36-37 0.6837087853195882 1104 38-39 0.6810792232690019 1104 40-41 0.5582154766119984 1104 42-43 0.5393261727054295 1104 44-45 0.5865219086345945 1104 46-47 0.6623265541544683 1104 48-49 0.7759041515669907 1104 50-51 0.5828924903018518 1104 52-53 0.6706833341226357 1104 54-55 0.7743037163724793 1104 56-57 0.7354909967765764 1104 58-59 0.604696683615245 1104 60-61 0.607794397945284 1104 62-63 0.6376711083727784 1104 64-65 0.7047014960312481 1104 66-67 0.8367709945958737 1104 68-69 0.6978888503939729 1104 70-71 0.5429948510239235 1104 72-73 0.6547015622517129 1104 74-75 0.6385426502873379 1104 76-77 0.6171832132061716 1104 78-79 0.6594794344782677 1104 80-81 0.6669540291614737 1104 82-83 0.785410769196087 1104 84-85 0.7994359157310633 1104 86-87 0.8650788883336666 1104 88-89 0.8734311361866176 1104 90-91 0.875261663938737 1104 92-93 0.7786153783362586 1104 94-95 0.778970029018943 1104 96-97 0.7075363271563262 1104 98-99 0.5758913477011127 1104 100-101 0.6446246245651075 1105 1 0.17192512298236196 1105 2 0.12885286715125943 1105 3 0.18069557590499485 1105 4 0.22012683735724892 1105 5 0.2533721152581663 1105 6 0.2630617541636653 1105 7 0.2772820290285978 1105 8 0.2707431605183359 1105 9 0.2219918210879328 1105 10-11 0.2786440724615389 1105 12-13 0.28647806581128066 1105 14-15 0.40192145960829606 1105 16-17 0.3407296709527827 1105 18-19 0.44617884705234445 1105 20-21 0.4134842066789304 1105 22-23 0.42267560081118916 1105 24-25 0.3692430114369074 1105 26-27 0.23957329699776864 1105 28-29 0.3344849572724371 1105 30-31 0.3901217140689326 1105 32-33 0.3847935294266094 1105 34-35 0.41436947337358276 1105 36-37 0.5197928718748557 1105 38-39 0.4772415993990009 1105 40-41 0.4430260856172836 1105 42-43 0.42501593564988127 1105 44-45 0.5653351519415608 1105 46-47 0.4268776262988112 1105 48-49 0.582370041215782 1105 50-51 0.3851436868929383 1105 52-53 0.3943411074930552 1105 54-55 0.4864522207047344 1105 56-57 0.4366143671658449 1105 58-59 0.2735400621197712 1105 60-61 0.5248322243177554 1105 62-63 0.4132713316058272 1105 64-65 0.4463643863177609 1105 66-67 0.5190993683886163 1105 68-69 0.43260541331289204 1105 70-71 0.2989921851207491 1105 72-73 0.3834514445360995 1105 74-75 0.34356217053852944 1105 76-77 0.21776670344099003 1105 78-79 0.24572421469926198 1105 80-81 0.3671979295965002 1105 82-83 0.4390280673556788 1105 84-85 0.26880267840311234 1105 86-87 0.2999590838585142 1105 88-89 0.2718077983469094 1105 90-91 0.3061569093655194 1105 92-93 0.4038867562820698 1105 94-95 0.4004745072685729 1105 96-97 0.32722956500254696 1105 98-99 0.3282944843403435 1105 100-101 0.3925322887523208 1106 1 0.26793678183987524 1106 2 0.2582783379480027 1106 3 0.2781134402954457 1106 4 0.2781932675482608 1106 5 0.18540272440928618 1106 6 0.3298599270885063 1106 7 0.22569573866451975 1106 8 0.2852572104832731 1106 9 0.3327791783825589 1106 10-11 0.28990999225202785 1106 12-13 0.3414202148380667 1106 14-15 0.48063130323303227 1106 16-17 0.46210356103934913 1106 18-19 0.6038063960323754 1106 20-21 0.39711939678637975 1106 22-23 0.5962930025426516 1106 24-25 0.4886954593071664 1106 26-27 0.29113664661902305 1106 28-29 0.3759308192894508 1106 30-31 0.3853793355107129 1106 32-33 0.5193315307670971 1106 34-35 0.4181498907834964 1106 36-37 0.4675735410129178 1106 38-39 0.4800704409968901 1106 40-41 0.5735648024609432 1106 42-43 0.4280543440335549 1106 44-45 0.5758886686644331 1106 46-47 0.6411981059959402 1106 48-49 0.6592347050936027 1106 50-51 0.5317589042936746 1106 52-53 0.42987538809306614 1106 54-55 0.47409801067541224 1106 56-57 0.4615511096175595 1106 58-59 0.4795708733974422 1106 60-61 0.7323594540494511 1106 62-63 0.6874814105281857 1106 64-65 0.6030113735472113 1106 66-67 0.5992413033844741 1106 68-69 0.47643602754818204 1106 70-71 0.5181127052431869 1106 72-73 0.525919347646802 1106 74-75 0.4501532319570991 1106 76-77 0.3279529512716941 1106 78-79 0.39008596913287263 1106 80-81 0.6036247600972722 1106 82-83 0.6036914491559813 1106 84-85 0.6134783490013263 1106 86-87 0.7859483351904721 1106 88-89 0.8395716525405064 1106 90-91 0.8494267154418438 1106 92-93 0.672556645813092 1106 94-95 0.6303653961355558 1106 96-97 0.6628715430897962 1106 98-99 0.7380710229046592 1106 100-101 0.7131305647244943 1107 1 0.1360540718521399 1107 2 0.12710772295238115 1107 3 0.21531259096931166 1107 4 0.3296549459678886 1107 5 0.26243867995677306 1107 6 0.23785947747432346 1107 7 0.03278500560811892 1107 8 0.20696690547439545 1107 9 0.3071755651643997 1107 10-11 0.25488686923683446 1107 12-13 0.37002722610188243 1107 14-15 0.34440515347445455 1107 16-17 0.3554401614867331 1107 18-19 0.3783903485116227 1107 20-21 0.34307768642437964 1107 22-23 0.3297463668054945 1107 24-25 0.36576952089195913 1107 26-27 0.40348445407051514 1107 28-29 0.40617926200626187 1107 30-31 0.4276856833465743 1107 32-33 0.49556590080697305 1107 34-35 0.5040661896682295 1107 36-37 0.4013264730779511 1107 38-39 0.5782910681592668 1107 40-41 0.45317473745216574 1107 42-43 0.6373968388734852 1107 44-45 0.34347781641086783 1107 46-47 0.47822408109670533 1107 48-49 0.5090944477018269 1107 50-51 0.3960989410780442 1107 52-53 0.49433002091020484 1107 54-55 0.5641219798052006 1107 56-57 0.5320441906234663 1107 58-59 0.6191448886620918 1107 60-61 0.6646230391296726 1107 62-63 0.6469387793217507 1107 64-65 0.5629246869443563 1107 66-67 0.5235234646946765 1107 68-69 0.5308035229130823 1107 70-71 0.40894894439346885 1107 72-73 0.46927243806495156 1107 74-75 0.5119585070184591 1107 76-77 0.5393163939329142 1107 78-79 0.5116232296731731 1107 80-81 0.41587825973844517 1107 82-83 0.4743328781385543 1107 84-85 0.3712582801510642 1107 86-87 0.4960103362453374 1107 88-89 0.4872953880932549 1107 90-91 0.528243001109896 1107 92-93 0.45307606136406164 1107 94-95 0.5402944892612744 1107 96-97 0.600486803044614 1107 98-99 0.6505733543518382 1107 100-101 0.7483196682506303 1108 1 0.36080556191174296 1108 2 0.30647802276437375 1108 3 0.303613623010591 1108 4 0.2971242697408414 1108 5 0.2143373809048157 1108 6 0.3442268571695095 1108 7 0.3102804804271102 1108 8 0.3553703416118381 1108 9 0.43858457152465036 1108 10-11 0.47884364000767476 1108 12-13 0.5098850079131623 1108 14-15 0.6528174899679087 1108 16-17 0.6776436746272552 1108 18-19 0.5860411545438566 1108 20-21 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2316 76-77 -2.720484385695169 2316 78-79 -2.6354879842327463 2316 80-81 -2.749485835445519 2316 82-83 -2.61792697504627 2316 84-85 -2.625146924894345 2316 86-87 -2.501524351963898 2316 88-89 -2.6639958961162513 2316 90-91 -2.7921190381778764 2316 92-93 -2.9628513896874686 2316 94-95 -3.0361808178130225 2316 96-97 -3.0197244201329987 2316 98-99 -3.2251590586656143 2316 100-101 -3.0333575096892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11722.0 3 3969.0 4 4132.0 5 4214.0 6 4833.0 7 5675.0 8 6852.0 9 8130.0 10 9751.0 11 11995.0 12 14479.0 13 18762.0 14 16885.0 15 17301.0 16 20671.0 17 25567.0 18 23048.0 19 18857.0 20 19214.0 21 20856.0 22 22720.0 23 25116.0 24 28296.0 25 32017.0 26 37133.0 27 42527.0 28 49480.0 29 57846.0 30 68284.0 31 81507.0 32 101033.0 33 127673.0 34 168954.0 35 235223.0 36 347970.0 37 490550.0 38 391992.0 39 54453.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09210739638571 19.096912409224128 14.54740906581868 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44-45 22.44924903144746 26.71229408764997 28.3088462182936 22.52961066260897 46-47 22.731184110474693 26.85845239141483 27.920231403821834 22.49013209428865 48-49 22.647164203061806 26.859665767596596 27.95305344641934 22.540116582922252 50-51 22.442687617131728 27.105867974771392 27.947477597840344 22.503966810256536 52-53 22.66892290830464 27.12431226475987 27.63639962839272 22.570365198542767 54-55 22.47823056570724 26.972107458543693 27.781286922443186 22.76837505330588 56-57 22.57171105990977 27.238741679729124 27.562937598589215 22.62660966177189 58-59 22.702680713537866 26.930282960599268 27.693156256481576 22.673880069381287 60-61 22.558749906455272 26.933378591162967 28.067484311212997 22.440387191168767 62-63 22.40650543185984 26.891016058207303 28.11536003683329 22.587118473099565 64-65 22.3655730697271 27.073303142518697 27.881514779122895 22.679609008631306 66-67 22.372174835684483 27.094134176862966 27.614122649168216 22.91956833828433 68-69 22.405782719502017 26.936151782816598 27.89775309718918 22.760312400492204 70-71 22.486158204994076 26.80160262652468 27.76879381196412 22.943445356517124 72-73 22.443548336238432 26.729534808745914 27.79576173221313 23.03115512280252 74-75 22.239793511970603 27.201292958140538 27.491107759507035 23.067805770381824 76-77 22.443233816340573 26.88592349963145 27.574286197935276 23.0965564860927 78-79 22.456008042802747 26.930628916076454 27.649292997829413 22.964070043291382 80-81 22.404567939794585 26.922992428475506 27.617257383119902 23.05518224861001 82-83 22.419845403170648 26.829504075074606 27.75209867297383 22.998551848780917 84-85 22.39255628268075 26.847770577852142 27.595034847049927 23.164638292417177 86-87 22.320649188752874 26.773610228830435 27.798633035454152 23.107107546962542 88-89 22.411866318394587 26.661710457658327 27.577099715906435 23.349323508040655 90-91 22.309011273000845 26.626203893927205 27.71161694460459 23.353167888467365 92-93 22.434475681233145 26.643798050370837 27.667076263331797 23.25465000506422 94-95 22.36638039525293 26.529786159324694 27.685478706234385 23.418354739187993 96-97 22.262310689485755 26.3031517497756 27.89405662780527 23.540480932933374 98-99 22.121588020569867 26.485279214203445 27.691844922213242 23.70128784301345 100-101 22.480975770808957 25.731621757741717 28.181595505566452 23.605806965882874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1980.0 1 1175.0 2 413.0 3 467.5 4 438.0 5 457.5 6 764.0 7 1535.5 8 2472.0 9 3002.0 10 2882.5 11 2621.5 12 2792.5 13 3141.0 14 3413.0 15 3605.5 16 3829.5 17 4038.5 18 4239.5 19 4490.0 20 4874.0 21 5612.0 22 6647.0 23 7971.0 24 9526.5 25 11531.5 26 14655.0 27 18586.0 28 22117.5 29 25475.0 30 29288.5 31 33887.0 32 38640.5 33 43256.0 34 48226.5 35 53217.0 36 58799.0 37 63899.5 38 67669.5 39 70574.5 40 73626.5 41 77810.0 42 80552.0 43 83350.5 44 87874.0 45 91099.5 46 93944.0 47 98874.0 48 107447.0 49 108633.0 50 101826.5 51 98196.5 52 95991.0 53 94963.0 54 92159.5 55 85185.5 56 77755.0 57 70371.5 58 63443.5 59 56955.5 60 48780.5 61 39850.0 62 31669.0 63 23897.0 64 17303.5 65 12716.5 66 9328.5 67 7092.5 68 5280.0 69 3854.5 70 2850.0 71 1830.0 72 1218.5 73 834.5 74 569.5 75 402.0 76 263.5 77 157.0 78 111.0 79 86.5 80 61.0 81 52.0 82 39.5 83 30.5 84 26.5 85 22.5 86 18.0 87 12.5 88 12.0 89 10.5 90 8.0 91 9.0 92 6.0 93 3.5 94 2.5 95 1.5 96 1.0 97 2.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31440884634237326 2 0.0862076505392066 3 0.09617077556844002 4 0.10122839247259285 5 0.11183797981288339 6 0.058181607995141646 7 0.059930858954472706 8 0.0608435116289063 9 0.06388568721035161 10-11 0.06118575638181889 12-13 0.05943650542248784 14-15 0.05778232245007695 16-17 0.05913228786434331 18-19 0.06707997157086919 20-21 0.06858204576420782 22-23 0.082994352581305 24-25 0.09193074335180061 26-27 0.08687312644764777 28-29 0.08432530439818732 30-31 0.07848813000128912 32-33 0.07093973183982794 34-35 0.31575881175663967 36-37 0.49098812524788976 38-39 0.4633423546515055 40-41 0.4448611379942252 42-43 0.4754350025877506 44-45 0.4858544539542008 46-47 0.48016938833637485 48-49 0.4983273738381266 50-51 0.4937831240633427 52-53 0.4926803354150687 54-55 0.48389605342364533 56-57 0.46180225326339874 58-59 0.44491817878637735 60-61 0.4041910531897781 62-63 0.4753209210034464 64-65 0.49882172737011143 66-67 0.5091461107496414 68-69 0.5208204595434379 70-71 0.5177972975593765 72-73 0.506579275102797 74-75 0.5084616212438162 76-77 0.4825651016067631 78-79 0.4639888169625626 80-81 0.46866616191903476 82-83 0.4778877566502909 84-85 0.4798081299860782 86-87 0.47855323255873206 88-89 0.4858924811489689 90-91 0.49950621687593666 92-93 0.5058377448048197 94-95 0.5123594087075432 96-97 0.504297643416713 98-99 0.5074919277772306 100-101 0.4856453043829765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2629697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.21774658749646 #Duplication Level Percentage of deduplicated Percentage of total 1 76.98844718792759 44.82093908551622 2 12.877399784379277 14.99386394705748 3 4.300228245417371 7.510487947803091 4 2.0075476101946568 4.674995921305865 5 1.038460550971491 3.022841659878511 6 0.6245102840305391 2.181454887418522 7 0.4291835716918783 1.7490270291392072 8 0.2910581706001861 1.3555800654577554 9 0.22750873818762188 1.1920541459623246 >10 1.1237555267616222 11.559858603850481 >50 0.06348532297445564 2.5109037323527343 >100 0.02657868090733184 2.738789817479305 >500 0.0011804952575167089 0.4577518341579764 >1k 5.246645588875273E-4 0.6584310934979428 >5k 1.3116613972188182E-4 0.5730202291224776 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8277 0.3147510910952859 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6731 0.25596104798385516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4334 0.16480986212479992 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3696 0.14054851186277353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8027194768066435E-5 2 0.0 0.0 0.0 3.8027194768066435E-5 3.8027194768066435E-5 3 0.0 0.0 0.0 3.8027194768066435E-5 3.8027194768066435E-5 4 0.0 0.0 0.0 3.8027194768066435E-5 3.8027194768066435E-5 5 0.0 0.0 0.0 3.8027194768066435E-5 3.8027194768066435E-5 6 0.0 0.0 0.0 3.8027194768066435E-5 1.1408158430419931E-4 7 0.0 0.0 0.0 3.8027194768066435E-5 1.1408158430419931E-4 8 0.0 0.0 0.0 3.8027194768066435E-5 1.1408158430419931E-4 9 0.0 0.0 0.0 3.8027194768066435E-5 1.1408158430419931E-4 10-11 0.0 0.0 0.0 3.8027194768066435E-5 1.1408158430419931E-4 12-13 0.0 0.0 0.0 7.605438953613287E-5 1.1408158430419931E-4 14-15 0.0 0.0 0.0 1.1408158430419931E-4 1.5210877907226574E-4 16-17 1.9013597384033217E-5 0.0 0.0 1.3309518168823253E-4 1.7112237645629897E-4 18-19 3.8027194768066435E-5 0.0 0.0 1.9013597384033218E-4 2.2816316860839862E-4 20-21 3.8027194768066435E-5 0.0 0.0 1.9013597384033218E-4 2.2816316860839862E-4 22-23 3.8027194768066435E-5 0.0 0.0 2.2816316860839862E-4 2.2816316860839862E-4 24-25 3.8027194768066435E-5 0.0 0.0 4.5632633721679725E-4 2.2816316860839862E-4 26-27 3.8027194768066435E-5 0.0 0.0 9.887070639697272E-4 2.2816316860839862E-4 28-29 3.8027194768066435E-5 0.0 0.0 0.002566835646844484 2.2816316860839862E-4 30-31 3.8027194768066435E-5 0.0 0.0 0.0069019358504040585 2.2816316860839862E-4 32-33 3.8027194768066435E-5 0.0 0.0 0.0169981560613257 2.2816316860839862E-4 34-35 3.8027194768066435E-5 0.0 0.0 0.0329695778639136 2.2816316860839862E-4 36-37 3.8027194768066435E-5 0.0 0.0 0.05882807030619878 2.2816316860839862E-4 38-39 3.8027194768066435E-5 0.0 0.0 0.09708342824287361 2.4717676599243186E-4 40-41 3.8027194768066435E-5 0.0 0.0 0.13735422750225595 2.6619036337646506E-4 42-43 3.8027194768066435E-5 0.0 0.0 0.1813897190436769 2.8520396076049827E-4 44-45 3.8027194768066435E-5 0.0 0.0 0.23768898089779925 3.422447529125979E-4 46-47 3.8027194768066435E-5 0.0 0.0 0.3061759586750869 3.422447529125979E-4 48-49 3.8027194768066435E-5 0.0 0.0 0.38261062015890046 3.422447529125979E-4 50-51 3.8027194768066435E-5 0.0 0.0 0.46474936085792395 3.422447529125979E-4 52-53 3.8027194768066435E-5 0.0 0.0 0.5490746652561113 3.6125835029663116E-4 54-55 3.8027194768066435E-5 0.0 0.0 0.6437243530338286 3.8027194768066436E-4 56-57 3.8027194768066435E-5 0.0 0.0 0.7611143032828498 3.9928554506469757E-4 58-59 3.8027194768066435E-5 0.0 0.0 0.8915856085320857 4.182991424487308E-4 60-61 3.8027194768066435E-5 0.0 0.0 1.0350812279893844 4.182991424487308E-4 62-63 3.8027194768066435E-5 0.0 0.0 1.1822464717418013 4.182991424487308E-4 64-65 3.8027194768066435E-5 0.0 0.0 1.3388044326019308 4.182991424487308E-4 66-67 3.8027194768066435E-5 0.0 0.0 1.522475783331692 4.37312739832764E-4 68-69 3.8027194768066435E-5 3.8027194768066435E-5 0.0 1.7364548082916018 4.5632633721679725E-4 70-71 3.8027194768066435E-5 3.8027194768066435E-5 0.0 1.971291749581796 4.5632633721679725E-4 72-73 3.8027194768066435E-5 3.8027194768066435E-5 0.0 2.2104257638807816 4.753399346008304E-4 74-75 3.8027194768066435E-5 3.8027194768066435E-5 0.0 2.469143783485322 4.943535319848636E-4 76-77 3.8027194768066435E-5 3.8027194768066435E-5 0.0 2.750221793613485 5.323807267529301E-4 78-79 3.8027194768066435E-5 3.8027194768066435E-5 0.0 3.069840365639083 5.323807267529301E-4 80-81 3.8027194768066435E-5 3.8027194768066435E-5 0.0 3.417751170572123 5.323807267529301E-4 82-83 3.8027194768066435E-5 3.8027194768066435E-5 0.0 3.7786292489210735 5.323807267529301E-4 84-85 3.8027194768066435E-5 3.8027194768066435E-5 0.0 4.155916061812444 5.323807267529301E-4 86-87 3.8027194768066435E-5 3.8027194768066435E-5 0.0 4.550733411491894 5.323807267529301E-4 88-89 3.8027194768066435E-5 3.8027194768066435E-5 0.0 4.981790677785311 5.323807267529301E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10400 0.0 28.477057 1 GTATCAA 14345 0.0 25.310732 1 GAGTACT 7785 0.0 22.196146 12-13 GTACATG 9770 0.0 21.471952 1 TACATGG 9630 0.0 21.44381 2 ATCAACG 16795 0.0 21.376223 3 TATCAAC 16915 0.0 21.280575 2 CAACGCA 17050 0.0 21.112076 5 TCAACGC 17020 0.0 21.093634 4 AACGCAG 17500 0.0 20.58607 6 GTACTTT 8325 0.0 20.55795 14-15 ACATGGG 9690 0.0 20.382338 3 AGAGTAC 13950 0.0 18.66463 10-11 AGTACTT 8390 0.0 18.42318 12-13 ACGCAGA 19370 0.0 18.354273 7 CGCAGAG 19270 0.0 18.351252 8 CATGGGG 5170 0.0 17.864292 4 ACTTTTT 10105 0.0 16.961699 16-17 GCAGAGT 20385 0.0 16.511469 9 GCGTAGA 575 0.0 16.508438 1 >>END_MODULE