FastQCFastQC Report
Fri 27 May 2016
SRR938953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629697
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84100.3198087079994387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75250.2861546406296999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45600.17340400814238296No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42650.16218598568580336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117250.025.3140721
GTATCAA155000.023.6364061
GTACATG96950.021.9166341
TACATGG94850.021.362712
GAGTACT83850.020.79951112-13
TATCACG1605.03044E-520.760792
ACATGGG95300.020.3157623
ATCAACG181350.019.965163
TATCAAC181850.019.9102672
TCAACGC181750.019.8951114
CAACGCA183150.019.7430325
AGTACTT87500.019.60645712-13
GTATAGG7100.019.4592671
GTACTTT90350.019.38193114-15
AACGCAG187400.019.2699646
AGAGTAC143050.018.76046610-11
TTTGCGT2905.2796167E-817.9995043
CGCAGAG205450.017.1150388
ACGCAGA207400.017.114287
TATAGCG1952.2939069E-417.0344945
Produced by FastQC (version 0.11.5)