##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938953_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2629697 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15530572533642 33.0 31.0 34.0 30.0 34.0 2 32.39697767461422 34.0 31.0 34.0 31.0 34.0 3 32.48423677708877 34.0 31.0 34.0 30.0 34.0 4 36.04618935185309 37.0 35.0 37.0 35.0 37.0 5 35.96897399206068 37.0 35.0 37.0 35.0 37.0 6 36.00482070748075 37.0 35.0 37.0 35.0 37.0 7 35.940848318266326 37.0 35.0 37.0 35.0 37.0 8 35.903937982208596 37.0 35.0 37.0 35.0 37.0 9 37.578952632185384 39.0 37.0 39.0 35.0 39.0 10-11 37.48355628043839 39.0 37.0 39.0 34.5 39.0 12-13 37.389873624223625 39.0 37.0 39.0 34.0 39.0 14-15 38.701789787948954 40.0 38.0 41.0 34.0 41.0 16-17 38.789630136095525 40.0 38.0 41.0 34.5 41.0 18-19 38.819224230015855 40.0 38.0 41.0 34.0 41.0 20-21 38.78955046912249 40.0 38.0 41.0 34.0 41.0 22-23 38.71416744970998 40.0 38.0 41.0 34.0 41.0 24-25 38.64200799559797 40.0 38.0 41.0 34.0 41.0 26-27 38.47836309658489 40.0 38.0 41.0 34.0 41.0 28-29 38.31758906064083 40.0 38.0 41.0 34.0 41.0 30-31 38.118990324740835 40.0 37.5 41.0 33.0 41.0 32-33 38.02496599418107 40.0 37.0 41.0 33.0 41.0 34-35 37.93376461242493 40.0 37.0 41.0 33.0 41.0 36-37 37.80615257195031 40.0 37.0 41.0 33.0 41.0 38-39 37.664324635119556 40.0 37.0 41.0 33.0 41.0 40-41 37.490265418411326 40.0 37.0 41.0 32.0 41.0 42-43 37.30321820346603 40.0 37.0 41.0 31.5 41.0 44-45 37.04412010204978 40.0 36.0 41.0 31.0 41.0 46-47 36.73710108046669 39.5 36.0 41.0 30.5 41.0 48-49 36.399610677579965 39.0 35.5 41.0 30.0 41.0 50-51 36.12490773651869 39.0 35.0 40.5 29.5 41.0 52-53 35.85242292172825 39.0 35.0 40.0 28.5 41.0 54-55 35.53652664166252 38.0 35.0 40.0 28.0 41.0 56-57 35.3749506121808 38.0 34.5 40.0 28.0 41.0 58-59 35.08144569507438 38.0 34.0 40.0 27.0 41.0 60-61 34.70235563260711 38.0 34.0 40.0 26.0 41.0 62-63 34.26141966165684 37.0 33.5 40.0 26.0 41.0 64-65 33.829874506454544 36.5 33.0 39.0 25.5 41.0 66-67 33.3534669583606 36.0 32.5 39.0 23.5 40.0 68-69 32.84364548463188 35.5 32.0 38.5 22.5 40.0 70-71 32.29737361376615 35.0 31.0 38.0 21.5 40.0 72-73 31.76044008111961 35.0 31.0 37.0 20.0 39.0 74-75 31.217875861743767 34.5 30.5 36.5 18.5 39.0 76-77 29.350858482935486 32.5 28.5 35.0 14.0 37.0 78-79 30.297927099585998 34.0 30.0 35.5 17.0 37.5 80-81 30.27337008788465 34.0 30.0 35.0 14.0 37.0 82-83 29.835925583822014 34.0 29.5 35.0 8.5 37.0 84-85 29.352051586171335 34.0 29.0 35.0 4.5 36.0 86-87 28.614944231217514 33.5 28.5 35.0 2.0 36.0 88-89 27.891143162120958 33.0 27.0 35.0 2.0 35.0 90-91 27.266536981256777 32.5 26.0 34.5 2.0 35.0 92-93 26.801240218930168 32.0 25.0 34.0 2.0 35.0 94-95 26.177768389285916 32.0 24.5 34.0 2.0 35.0 96-97 25.249333668479675 32.0 21.5 34.0 2.0 35.0 98-99 23.922471676394657 31.0 6.0 34.0 2.0 35.0 100-101 18.30680435806863 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19869530582344908 1101 2 0.1844502031799209 1101 3 0.19348530560095867 1101 4 0.15853612762933977 1101 5 0.18613242187485213 1101 6 0.16023022591922853 1101 7 0.20834359951083314 1101 8 0.1516569941618613 1101 9 0.23156013349031213 1101 10-11 0.2694792410673159 1101 12-13 0.2972583238704942 1101 14-15 0.32330037413846213 1101 16-17 0.2742416781853123 1101 18-19 0.3463221692750693 1101 20-21 0.334009906187255 1101 22-23 0.4098656302045498 1101 24-25 0.31362071511775014 1101 26-27 0.252690391599927 1101 28-29 0.29892562132122436 1101 30-31 0.25748748807466626 1101 32-33 0.4552619318697353 1101 34-35 0.39570044534271176 1101 36-37 0.4332323367145179 1101 38-39 0.4552769747341827 1101 40-41 0.4698319667199726 1101 42-43 0.4836354942345764 1101 44-45 0.3727124767167638 1101 46-47 0.2976814550341871 1101 48-49 0.31635774453190635 1101 50-51 0.25877585451718943 1101 52-53 0.20588649250654356 1101 54-55 0.20128650526323355 1101 56-57 0.18628093788331768 1101 58-59 0.18558571109200983 1101 60-61 0.22069524418656528 1101 62-63 0.27636275049965064 1101 64-65 0.3139614464629048 1101 66-67 0.13600068516884534 1101 68-69 0.2685062803867311 1101 70-71 0.27340808494989943 1101 72-73 0.38465434457033965 1101 74-75 0.4488204040323822 1101 76-77 0.13526437089180376 1101 78-79 0.4000840298253472 1101 80-81 0.6115213170433194 1101 82-83 0.594395824422655 1101 84-85 0.7066172866135858 1101 86-87 0.8620559413829874 1101 88-89 0.9352200782040008 1101 90-91 0.9622887662728488 1101 92-93 0.8227192859047108 1101 94-95 0.7450035862686413 1101 96-97 0.9881115875483601 1101 98-99 1.0551886404882111 1101 100-101 1.2348244032334819 1102 1 0.15073659717182153 1102 2 0.13679861860642006 1102 3 0.1446102267357645 1102 4 0.06025875690490068 1102 5 0.054221129712814786 1102 6 0.06089209331365453 1102 7 0.11678460967894466 1102 8 0.11712871140229453 1102 9 0.16008659151268745 1102 10-11 0.2539184545095452 1102 12-13 0.2990833945323743 1102 14-15 0.4284418512952328 1102 16-17 0.28874770528693006 1102 18-19 0.26771557887921915 1102 20-21 0.2920352817723213 1102 22-23 0.2458478670282247 1102 24-25 0.273995539880282 1102 26-27 0.31420340952583814 1102 28-29 0.28081963983803604 1102 30-31 0.29106622054924713 1102 32-33 0.3048493318459933 1102 34-35 0.3553371385667745 1102 36-37 0.271344767469877 1102 38-39 0.39433368697926596 1102 40-41 0.3643150045339141 1102 42-43 0.42855857649314544 1102 44-45 0.2487827424374487 1102 46-47 0.37519593755178704 1102 48-49 0.5095101114214557 1102 50-51 0.36664543382723735 1102 52-53 0.3713338332321996 1102 54-55 0.3464694925234184 1102 56-57 0.3775893931659198 1102 58-59 0.343818136992077 1102 60-61 0.4193084839354242 1102 62-63 0.4241556254878205 1102 64-65 0.4733520069159951 1102 66-67 0.35263037022827604 1102 68-69 0.32752945908426057 1102 70-71 0.35954371707861554 1102 72-73 0.20827742162680707 1102 74-75 0.3233157001320066 1102 76-77 0.12190897531198885 1102 78-79 0.3304085475971945 1102 80-81 0.4203124019749609 1102 82-83 0.45676822287163077 1102 84-85 0.6428061735905892 1102 86-87 0.892327837895845 1102 88-89 0.6765932596359683 1102 90-91 0.8210181929612297 1102 92-93 0.9636578917681469 1102 94-95 1.0428700910032234 1102 96-97 1.073815712014646 1102 98-99 1.3505387952840806 1102 100-101 1.4953891713998182 1103 1 0.08399634574507786 1103 2 0.1368708124754363 1103 3 0.07613583221216658 1103 4 0.05260620678894412 1103 5 0.10043249150444211 1103 6 0.08504988394282265 1103 7 0.1190035776576508 1103 8 0.1275834102462312 1103 9 0.0632247728961417 1103 10-11 0.13861110235878016 1103 12-13 0.11138421976127688 1103 14-15 0.16388518291645227 1103 16-17 0.1267269459067819 1103 18-19 0.14935712505994303 1103 20-21 0.10846755017985288 1103 22-23 0.1589313334069118 1103 24-25 0.13155720188913023 1103 26-27 0.20826536444970856 1103 28-29 0.20752597339292578 1103 30-31 0.26684699889850805 1103 32-33 0.3410029782821553 1103 34-35 0.1671195257133533 1103 36-37 0.2118944409913226 1103 38-39 0.24473524053833984 1103 40-41 0.254717922490876 1103 42-43 0.2872235131562775 1103 44-45 0.1797583664093949 1103 46-47 0.2563059646509842 1103 48-49 0.3857372830080763 1103 50-51 0.37080767530778047 1103 52-53 0.44128397972282585 1103 54-55 0.49644066465049264 1103 56-57 0.4326241372932955 1103 58-59 0.3987364767847694 1103 60-61 0.19191617615904732 1103 62-63 0.4560101766986051 1103 64-65 0.4148738967262986 1103 66-67 0.4136665608823549 1103 68-69 0.39535833113453833 1103 70-71 0.3929085441593969 1103 72-73 0.3419443701144935 1103 74-75 0.3361099108460408 1103 76-77 0.23584704489429598 1103 78-79 0.41096490070734504 1103 80-81 0.22359553407616772 1103 82-83 0.257112269708891 1103 84-85 0.4366631211079657 1103 86-87 0.576252730441233 1103 88-89 0.4209009552043561 1103 90-91 0.5305323348460256 1103 92-93 0.7447906698710192 1103 94-95 1.0098509996988412 1103 96-97 0.6453405458776729 1103 98-99 0.8750349532787105 1103 100-101 1.0024693036139496 1104 1 -0.0549561394976692 1104 2 -0.009732912658442672 1104 3 -0.03355025050058913 1104 4 0.05910631714652226 1104 5 -0.046238464841508176 1104 6 0.06780179847456935 1104 7 0.06761253108413001 1104 8 0.11594667130765401 1104 9 0.07274389263296399 1104 10-11 0.09246835376973195 1104 12-13 0.08867384427988156 1104 14-15 0.14325938495574775 1104 16-17 0.15294728259413404 1104 18-19 0.10440855037803942 1104 20-21 0.14146774465842782 1104 22-23 0.12532220452336418 1104 24-25 0.13782708633998908 1104 26-27 0.11400597528298562 1104 28-29 0.11758217212707223 1104 30-31 0.01719996276189306 1104 32-33 0.11410673834347307 1104 34-35 0.11923745647438722 1104 36-37 0.13745948653308204 1104 38-39 0.23623483764300346 1104 40-41 0.263297936980031 1104 42-43 0.12377279412665843 1104 44-45 0.20555342468573912 1104 46-47 0.3101309450801466 1104 48-49 0.14625527817988626 1104 50-51 0.09348958411011665 1104 52-53 0.25073170319200244 1104 54-55 0.4195063488887669 1104 56-57 0.09850903614640316 1104 58-59 0.18563739339216312 1104 60-61 0.24464577976710444 1104 62-63 0.1876721257019227 1104 64-65 0.27570118826936607 1104 66-67 0.19438302587177958 1104 68-69 0.12539320281639021 1104 70-71 0.32069672911016056 1104 72-73 0.20781072299176984 1104 74-75 0.050409973256282825 1104 76-77 0.06810049738101398 1104 78-79 0.4299692786070999 1104 80-81 0.34422079090759183 1104 82-83 0.22631283156505333 1104 84-85 0.4187987221992735 1104 86-87 0.4547825740483127 1104 88-89 0.5209833052402182 1104 90-91 0.6341591635759904 1104 92-93 0.8543719702800807 1104 94-95 0.9558909829205504 1104 96-97 0.5713968712032802 1104 98-99 0.6122037944651382 1104 100-101 0.6219705693644997 1105 1 0.053199071938536235 1105 2 0.05238016638387677 1105 3 0.006588720288867478 1105 4 0.041895820136971906 1105 5 0.03507000189758713 1105 6 0.0034042464537478168 1105 7 0.11807603039490289 1105 8 0.026732771447768755 1105 9 0.026643613465246574 1105 10-11 0.0719272158752915 1105 12-13 0.08358015529054086 1105 14-15 0.0397017188408455 1105 16-17 -0.009792896763379133 1105 18-19 0.009240610486635603 1105 20-21 0.09553574351824778 1105 22-23 0.03829339090084005 1105 24-25 -0.0852210939504019 1105 26-27 -0.02981453953085378 1105 28-29 0.08289082721115193 1105 30-31 0.030363096696028435 1105 32-33 0.1074789541716541 1105 34-35 0.17425678711654058 1105 36-37 0.22356395606069412 1105 38-39 0.16334153634095827 1105 40-41 0.2044345989633527 1105 42-43 0.12018744509116885 1105 44-45 0.14410666342944012 1105 46-47 -0.0018776909798319252 1105 48-49 0.039127905775714567 1105 50-51 0.16924234781995295 1105 52-53 0.09961613837509731 1105 54-55 0.06818922965691598 1105 56-57 0.0839631782507908 1105 58-59 -0.011232572092673365 1105 60-61 -0.08965487571136777 1105 62-63 -0.1674427046959437 1105 64-65 -0.3937171796644563 1105 66-67 -0.21807068582008782 1105 68-69 -0.18498953568177257 1105 70-71 0.024248043322053547 1105 72-73 -0.0057601651938732346 1105 74-75 0.019738972126845056 1105 76-77 -0.15232981402656165 1105 78-79 -0.13116889392828668 1105 80-81 -0.06604072420772411 1105 82-83 0.12958788539821953 1105 84-85 0.13163013510899546 1105 86-87 0.12875898541904363 1105 88-89 0.15433819080167765 1105 90-91 0.02965060809986042 1105 92-93 0.26829357199616766 1105 94-95 0.35624416136857917 1105 96-97 0.40510341834040275 1105 98-99 0.7435919602061993 1105 100-101 0.8216236796164722 1106 1 0.09170262868370571 1106 2 0.06897779381505131 1106 3 0.01735567892298917 1106 4 0.016091204583517538 1106 5 0.08438081858183466 1106 6 0.010760164638377034 1106 7 0.09994168320474017 1106 8 0.06851933449668479 1106 9 0.10256259172176385 1106 10-11 0.11764765130298827 1106 12-13 0.10443391053513551 1106 14-15 0.03372829042123726 1106 16-17 -0.013524797602336491 1106 18-19 0.04977240790623938 1106 20-21 0.03676249044031721 1106 22-23 0.12530638838170205 1106 24-25 0.14700572055385663 1106 26-27 0.11477042213019928 1106 28-29 0.1361215658224708 1106 30-31 0.1289831800143375 1106 32-33 0.20489666355708636 1106 34-35 0.13238977646273042 1106 36-37 0.10653749174378646 1106 38-39 0.025249424918385444 1106 40-41 0.14995994507405896 1106 42-43 0.14143367304403398 1106 44-45 0.2324063575735309 1106 46-47 0.07919706422074313 1106 48-49 0.2518155626020402 1106 50-51 0.20685106059007552 1106 52-53 0.26066232305055337 1106 54-55 0.3723823944315896 1106 56-57 0.3870179861654748 1106 58-59 0.4042236603801328 1106 60-61 0.4757445319214568 1106 62-63 0.4708625386278342 1106 64-65 0.38454116534074956 1106 66-67 0.516957069378627 1106 68-69 0.4505773889752547 1106 70-71 0.5569750280218244 1106 72-73 0.48877249777375553 1106 74-75 0.49778871137739955 1106 76-77 0.1743586091445799 1106 78-79 0.475156953902669 1106 80-81 0.5208013027371123 1106 82-83 0.5661485162314435 1106 84-85 0.5738151230589068 1106 86-87 0.6747065031996691 1106 88-89 0.7499090153849792 1106 90-91 1.0585043273832895 1106 92-93 1.0784910111892465 1106 94-95 1.0224414728375208 1106 96-97 1.029682037196082 1106 98-99 1.152881473000626 1106 100-101 1.0702134439530404 1107 1 0.0027951293908614616 1107 2 -0.006752551313240929 1107 3 0.04088260574827984 1107 4 0.02083253437923105 1107 5 0.06580335339296539 1107 6 0.056365433029640144 1107 7 0.0374274011607767 1107 8 0.08699674817755465 1107 9 0.05263935963808564 1107 10-11 0.0332335146425109 1107 12-13 0.050473878024575924 1107 14-15 0.1353932833867475 1107 16-17 0.08476175856299761 1107 18-19 0.151779706725506 1107 20-21 0.1266898048725622 1107 22-23 0.10312027528397039 1107 24-25 0.09798804725785004 1107 26-27 0.08047329014991078 1107 28-29 0.15025754693867555 1107 30-31 0.15153059022427584 1107 32-33 0.1684638642023515 1107 34-35 0.20649660059317654 1107 36-37 0.20009460695001025 1107 38-39 0.04296888666598164 1107 40-41 0.07132826061428688 1107 42-43 0.027939287662917422 1107 44-45 0.11335880469754755 1107 46-47 0.04305839777146758 1107 48-49 0.12406090372210343 1107 50-51 -0.04385933599680669 1107 52-53 0.025540661652030394 1107 54-55 0.14311513585526114 1107 56-57 0.28501392975618245 1107 58-59 0.17003519545436774 1107 60-61 0.30504106482789695 1107 62-63 0.3072537733746117 1107 64-65 0.28343618289917316 1107 66-67 0.2821828090495515 1107 68-69 0.34694409875053367 1107 70-71 0.38067099248748093 1107 72-73 0.6196591702952823 1107 74-75 0.46135452241839303 1107 76-77 0.3108534157301932 1107 78-79 0.5341309874483322 1107 80-81 0.4029721240117681 1107 82-83 0.28243887805969337 1107 84-85 0.502011336418132 1107 86-87 0.4300929181812059 1107 88-89 0.5155466955240158 1107 90-91 0.4994822752898038 1107 92-93 0.7226257076934992 1107 94-95 0.7070054705993343 1107 96-97 0.78133118867283 1107 98-99 0.9531883902654386 1107 100-101 0.9347508045448478 1108 1 0.1285064078419964 1108 2 0.07993404115045877 1108 3 0.12454095208213545 1108 4 0.08389755698013346 1108 5 0.08521350479902168 1108 6 0.1421076127168277 1108 7 0.07502390502092737 1108 8 0.16455422547664256 1108 9 0.2092606244886781 1108 10-11 0.19777728389328786 1108 12-13 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-0.6122541345487065 2316 46-47 -0.6739983852750484 2316 48-49 -0.6850204130189397 2316 50-51 -0.7016831295687069 2316 52-53 -0.6518955249427165 2316 54-55 -0.48909007483378986 2316 56-57 -0.5924962287896918 2316 58-59 -0.6887635363398701 2316 60-61 -0.6703327757071165 2316 62-63 -0.48291430621679154 2316 64-65 -0.5532810648605064 2316 66-67 -0.6411236968541019 2316 68-69 -0.5123542460514798 2316 70-71 -0.5058974547465311 2316 72-73 -0.4768601168327926 2316 74-75 -0.7371140364789994 2316 76-77 -0.5286161126633431 2316 78-79 -0.7347457172322542 2316 80-81 -0.7132782236765607 2316 82-83 -0.8385584230726622 2316 84-85 -1.022784887988987 2316 86-87 -1.10043512464593 2316 88-89 -1.2391332397354127 2316 90-91 -1.5041533352508942 2316 92-93 -1.2829687990325063 2316 94-95 -1.215985092434039 2316 96-97 -1.166239679278167 2316 98-99 -1.230256757617994 2316 100-101 -0.6820130132946858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 24.0 5 45.0 6 117.0 7 294.0 8 753.0 9 1413.0 10 2428.0 11 3787.0 12 5257.0 13 7396.0 14 9236.0 15 12361.0 16 16333.0 17 22954.0 18 23039.0 19 17031.0 20 16616.0 21 17844.0 22 19752.0 23 21647.0 24 24829.0 25 29353.0 26 35016.0 27 42550.0 28 50692.0 29 61556.0 30 74491.0 31 90105.0 32 110922.0 33 139859.0 34 186950.0 35 263407.0 36 403680.0 37 564597.0 38 337393.0 39 15965.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.13210506686639 19.792856005835947 15.14906511499538 22.925973812302285 2 15.75299359584013 20.853124903743662 39.585054856129815 23.808826644286395 3 19.360367373123218 24.49943852846925 30.217587805743403 25.922606292664135 4 13.342069447544716 17.41523833354185 36.70099635053012 32.541695868383314 5 13.823759923671814 37.320269217328075 34.293152405010915 14.562818453989184 6 31.75510334460586 36.3654443838967 17.32264211428161 14.556810157215832 7 28.205949202512688 32.83294615311193 20.774902964105753 18.18620168026963 8 25.981662526138944 35.512228214885596 19.58073496680416 18.925374292171306 9 25.948084513158744 17.12870342096447 20.45479003854817 36.46842202732862 10-11 24.97538689818637 26.760269339015103 27.14139689857805 21.12294686422048 12-13 25.941695944437704 23.984569324907014 27.660430079967384 22.41330465068789 14-15 22.792829604131462 25.429753921922245 26.103012408194402 25.674404065751887 16-17 22.119336986320125 28.519878659689258 26.904403624998054 22.45638072899257 18-19 22.060576145708875 27.27692930171785 28.967380846809615 21.695113705763664 20-21 22.958196324519516 26.32757690334666 28.962842487176278 21.751384284957545 22-23 22.525503888851073 26.304399328135524 28.86574004533602 22.304356737677384 24-25 22.487341687914377 26.400209910274764 29.030039605353476 22.082408796457383 26-27 22.297820299795053 26.552721958598646 28.979675030939884 22.16978271066642 28-29 22.12222548833573 26.945176573574827 28.92985389571498 22.002744042374463 30-31 22.40541400777352 26.561158947209506 28.783068923910243 22.25035812110673 32-33 22.245889925721478 26.563877891635425 28.825773463634786 22.36445871900831 34-35 22.626020218001475 26.611373956962588 28.725155362182143 22.037450462853798 36-37 22.6272365081119 26.390790965856564 28.811627531479285 22.170344994552256 38-39 22.33624731496863 26.69522079629055 28.829877781631648 22.138654107109172 40-41 22.5458228799324 26.59337591893903 28.771258100791513 22.089543100337057 42-43 22.30185759603005 26.633541917461685 28.521941644487686 22.542658842020575 44-45 22.50117743649496 26.722969395017255 28.387542313084214 22.388310855403574 46-47 22.750436747480478 26.856205312765958 27.968152609494894 22.42520533025867 48-49 22.691802646545106 26.951586485788614 27.914464349751313 22.442146517914967 50-51 22.524764429422756 27.149815623884134 27.927439052447085 22.39798089424603 52-53 22.729158530431455 27.180184637241478 27.60009993546785 22.49055689685922 54-55 22.54784486577731 27.05823142362029 27.694407378492652 22.699516332109745 56-57 22.615229054906326 27.3660425516704 27.508074124129127 22.510654269294143 58-59 22.691242557309785 27.046624538560337 27.687159356993398 22.574973547136484 60-61 22.5738001504001 27.032259965908047 28.026859062176467 22.36708082151539 62-63 22.490731695074004 27.000799722854012 28.027681134564492 22.480787447507492 64-65 22.40372303996039 27.091272356816177 27.867716934272206 22.637287668951227 66-67 22.42130629983123 27.18643259535767 27.56819791103374 22.824063193777366 68-69 22.41335643422786 27.052422703778184 27.760717882100256 22.773502979893703 70-71 22.53490089065411 26.906070719567825 27.64104146619201 22.91798692358605 72-73 22.490880128014748 26.802251362039048 27.67533674031647 23.03153176962973 74-75 22.267831617102654 27.281546124895755 27.355832249875178 23.094790008126413 76-77 22.337122374117094 26.958227176503634 27.576023767965463 23.12862668141381 78-79 22.306506110233286 27.067506336503012 27.58319215243354 23.042795400830162 80-81 22.34876018632618 27.02223969788068 27.501725964225056 23.127274151568084 82-83 22.33989315118814 26.92568763625619 27.644534712554336 23.089884500001332 84-85 22.23295647058421 26.926755328473707 27.529221091587498 23.311067109354592 86-87 22.167313311235706 26.875984501845156 27.640596426609186 23.31610576030995 88-89 22.15723125361385 26.802227927152405 27.49996371827354 23.540577100960206 90-91 22.109364278402552 26.722016791709617 27.57900973309897 23.589609196788857 92-93 22.121883243582815 26.785025042809114 27.506096710001188 23.586995003606877 94-95 22.03580483881548 26.605879629497593 27.578681375722642 23.77963415596429 96-97 21.884707629814386 26.464855076459383 27.742245589510883 23.908191704215355 98-99 21.71704572808198 26.641149151404136 27.513283849812353 24.12852127070153 100-101 21.984984124515364 25.769070688544836 28.254654980342693 23.991290206597103 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 560.0 1 415.0 2 316.5 3 378.5 4 366.0 5 372.0 6 597.0 7 1274.0 8 2107.5 9 2595.0 10 2481.0 11 2220.0 12 2305.0 13 2468.5 14 2690.0 15 2859.0 16 3022.5 17 3243.0 18 3473.0 19 3899.0 20 4452.0 21 5140.0 22 6169.5 23 7610.5 24 9304.5 25 11276.0 26 14433.0 27 18507.5 28 22068.0 29 25546.0 30 29387.5 31 34296.5 32 39222.5 33 43492.0 34 48502.5 35 53743.0 36 59526.5 37 64586.0 38 68461.0 39 71244.5 40 74233.5 41 78747.0 42 81788.0 43 84318.0 44 88584.5 45 92079.0 46 94656.0 47 100256.0 48 109490.0 49 110176.5 50 102975.5 51 99205.0 52 97173.0 53 95605.5 54 92619.0 55 85684.5 56 77922.5 57 70554.5 58 63575.0 59 56837.5 60 48277.0 61 39251.5 62 30817.0 63 22845.5 64 16480.0 65 11991.5 66 8493.0 67 6333.0 68 4670.0 69 3340.5 70 2362.5 71 1408.0 72 895.5 73 632.0 74 420.5 75 274.5 76 164.5 77 83.0 78 50.5 79 38.5 80 20.5 81 10.0 82 8.0 83 5.0 84 3.0 85 3.5 86 2.0 87 1.0 88 0.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.382933851314429 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.936390770495612E-4 16-17 0.0025858492442285175 18-19 5.704079215209964E-4 20-21 0.0 22-23 0.0 24-25 7.605438953613287E-5 26-27 1.9013597384033217E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013575708532199719 36-37 0.02382403752219362 38-39 0.011598294404260263 40-41 0.006464623110571294 42-43 0.005418875254449468 44-45 0.00165418297241089 46-47 0.0011788430378100595 48-49 0.003042175581445315 50-51 6.844895058251958E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.415302979772955E-4 60-61 0.0016922101671789564 62-63 0.0013689790116503916 64-65 6.844895058251958E-4 66-67 0.002699930828532717 68-69 0.0027759852180688498 70-71 1.9013597384033217E-5 72-73 0.0 74-75 0.0 76-77 0.08727241199271248 78-79 0.0034985019186621123 80-81 2.852039607604982E-4 82-83 0.0 84-85 1.5210877907226574E-4 86-87 0.09189271615703254 88-89 0.4296692736843827 90-91 0.385234496597897 92-93 0.0 94-95 9.316662718176276E-4 96-97 0.0 98-99 0.0 100-101 5.133671293688968E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2629697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.102490068561764 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30946171272575 42.25048327806928 2 13.794217858911976 15.477799408663728 3 4.586318629926578 7.719116861601469 4 2.0801543766474895 4.668073610277642 5 1.1325954032655083 3.177071118170094 6 0.7181921395061855 2.4175420424378937 7 0.4705490382659465 1.8479280922260588 8 0.3326493031529525 1.4929963381162006 9 0.25063100919653347 1.2654921333889917 >10 1.2222871226741971 12.198446027098186 >50 0.07094698553883148 2.695191351348451 >100 0.030027585447936284 2.9931128563274756 >500 0.0011541445029129968 0.4342465224816128 >1k 6.789085311135059E-4 0.7555607636478924 >5k 1.357817062227012E-4 0.6069395961450403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8410 0.3198087079994387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7525 0.2861546406296999 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4560 0.17340400814238296 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4265 0.16218598568580336 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.8027194768066435E-5 0.0 5 0.0 0.0 0.0 3.8027194768066435E-5 0.0 6 0.0 0.0 0.0 3.8027194768066435E-5 0.0 7 0.0 0.0 0.0 3.8027194768066435E-5 0.0 8 0.0 0.0 0.0 3.8027194768066435E-5 0.0 9 0.0 0.0 0.0 3.8027194768066435E-5 0.0 10-11 0.0 0.0 0.0 3.8027194768066435E-5 0.0 12-13 0.0 0.0 0.0 1.1408158430419931E-4 1.9013597384033217E-5 14-15 0.0 0.0 0.0 1.1408158430419931E-4 3.8027194768066435E-5 16-17 0.0 0.0 0.0 1.3309518168823253E-4 5.704079215209965E-5 18-19 1.9013597384033217E-5 0.0 0.0 1.9013597384033218E-4 7.605438953613287E-5 20-21 3.8027194768066435E-5 0.0 0.0 1.9013597384033218E-4 1.1408158430419931E-4 22-23 3.8027194768066435E-5 0.0 0.0 2.2816316860839862E-4 1.1408158430419931E-4 24-25 3.8027194768066435E-5 0.0 0.0 4.5632633721679725E-4 1.1408158430419931E-4 26-27 3.8027194768066435E-5 0.0 0.0 9.887070639697272E-4 1.1408158430419931E-4 28-29 3.8027194768066435E-5 0.0 0.0 0.0025858492442285175 1.1408158430419931E-4 30-31 3.8027194768066435E-5 0.0 0.0 0.006863908655635992 1.1408158430419931E-4 32-33 3.8027194768066435E-5 0.0 0.0 0.016941115269173596 1.1408158430419931E-4 34-35 3.8027194768066435E-5 0.0 0.0 0.03315971383775393 1.5210877907226574E-4 36-37 3.8027194768066435E-5 0.0 0.0 0.059455519019871866 1.7112237645629897E-4 38-39 3.8027194768066435E-5 0.0 0.0 0.09860451603359627 1.9013597384033218E-4 40-41 3.8027194768066435E-5 0.0 0.0 0.13952177760403575 1.9013597384033218E-4 42-43 3.8027194768066435E-5 0.0 0.0 0.18418471785912977 1.9013597384033218E-4 44-45 3.8027194768066435E-5 0.0 0.0 0.24139663238768572 2.091495712243654E-4 46-47 7.605438953613287E-5 0.0 0.0 0.3105110588786465 2.2816316860839862E-4 48-49 7.605438953613287E-5 0.0 0.0 0.3872309243232205 2.6619036337646506E-4 50-51 7.605438953613287E-5 0.0 0.0 0.47003514093068516 2.6619036337646506E-4 52-53 7.605438953613287E-5 0.0 0.0 0.5560146283012835 2.6619036337646506E-4 54-55 7.605438953613287E-5 0.0 0.0 0.6523184990514117 2.6619036337646506E-4 56-57 7.605438953613287E-5 0.0 0.0 0.7703168844167219 2.6619036337646506E-4 58-59 7.605438953613287E-5 0.0 0.0 0.9015677471587031 2.8520396076049827E-4 60-61 7.605438953613287E-5 0.0 0.0 1.0467365631857968 3.422447529125979E-4 62-63 7.605438953613287E-5 0.0 0.0 1.1954609219237047 3.8027194768066436E-4 64-65 7.605438953613287E-5 0.0 0.0 1.353121671432108 3.8027194768066436E-4 66-67 7.605438953613287E-5 0.0 0.0 1.5390556402505688 4.37312739832764E-4 68-69 7.605438953613287E-5 0.0 0.0 1.7544987122090492 4.5632633721679725E-4 70-71 7.605438953613287E-5 0.0 0.0 1.9918074211591679 4.5632633721679725E-4 72-73 7.605438953613287E-5 3.8027194768066435E-5 0.0 2.2338124886631423 5.323807267529301E-4 74-75 7.605438953613287E-5 3.8027194768066435E-5 0.0 2.494393840811318 5.323807267529301E-4 76-77 7.605438953613287E-5 3.8027194768066435E-5 0.0 2.77716406110666 5.323807267529301E-4 78-79 7.605438953613287E-5 3.8027194768066435E-5 0.0 3.0992544007921827 5.323807267529301E-4 80-81 1.7112237645629897E-4 3.8027194768066435E-5 0.0 3.449675000579915 5.323807267529301E-4 82-83 1.9013597384033218E-4 3.8027194768066435E-5 0.0 3.812758656225413 6.08435116289063E-4 84-85 1.9013597384033218E-4 3.8027194768066435E-5 0.0 4.1921559784264115 6.08435116289063E-4 86-87 1.9013597384033218E-4 3.8027194768066435E-5 0.0 4.588836660649497 6.08435116289063E-4 88-89 1.9013597384033218E-4 3.8027194768066435E-5 0.0 5.022403721797606 6.08435116289063E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11725 0.0 25.314072 1 GTATCAA 15500 0.0 23.636406 1 GTACATG 9695 0.0 21.916634 1 TACATGG 9485 0.0 21.36271 2 GAGTACT 8385 0.0 20.799511 12-13 TATCACG 160 5.03044E-5 20.76079 2 ACATGGG 9530 0.0 20.315762 3 ATCAACG 18135 0.0 19.96516 3 TATCAAC 18185 0.0 19.910267 2 TCAACGC 18175 0.0 19.895111 4 CAACGCA 18315 0.0 19.743032 5 AGTACTT 8750 0.0 19.606457 12-13 GTATAGG 710 0.0 19.459267 1 GTACTTT 9035 0.0 19.381931 14-15 AACGCAG 18740 0.0 19.269964 6 AGAGTAC 14305 0.0 18.760466 10-11 TTTGCGT 290 5.2796167E-8 17.999504 3 CGCAGAG 20545 0.0 17.115038 8 ACGCAGA 20740 0.0 17.11428 7 TATAGCG 195 2.2939069E-4 17.034494 5 >>END_MODULE