FastQCFastQC Report
Fri 27 May 2016
SRR938952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641092
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82420.312067887070954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69990.26500402106401444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42070.15929017239838672No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38330.14512936315736066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108900.026.9059351
GTATCAA151400.025.4596351
GTACATG93000.022.125561
GAGTACT80550.022.00409712-13
TATCAAC174100.021.7493112
TACATGG92800.021.62582
ATCAACG175050.021.496083
TCAACGC174800.021.4730784
CAACGCA176050.021.3479045
AACGCAG179600.020.8964146
ACATGGG92400.020.746143
ACCGTAC1150.003764802120.6138761
GTACTTT86250.020.46798914-15
AGTACTT84650.019.45671712-13
AGAGTAC134950.019.04356610-11
ACGCAGA195600.018.9209447
CGCAGAG194950.018.7173468
ATACCGA2102.0199423E-518.0291656
CATGGGG49600.017.2726674
ACTTTTT105850.017.12624516-17
Produced by FastQC (version 0.11.5)