FastQCFastQC Report
Fri 27 May 2016
SRR938952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641092
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81920.31017473075530877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77610.2938557233144472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.17322380288153535No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40740.1542543765987705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116900.026.849431
GTATCAA155700.024.0741141
GAGTACT82800.022.26435912-13
GTACATG96900.021.5776881
GTACTTT86800.020.82834614-15
TACATGG96700.020.657242
TATCAAC181000.020.4995732
ATCAACG181050.020.4677053
TCAACGC181050.020.4677054
CAACGCA181300.020.4394825
AACGCAG185300.020.0490936
ACATGGG96150.019.6897533
AGTACTT88050.018.70034612-13
AGAGTAC145200.018.36619610-11
ACGCAGA203350.017.9661467
CGCAGAG202750.017.83218
CATGGGG50750.017.7637334
ACTTTTT105500.017.24763516-17
GCAGAGT211400.016.3402799
TACTTTT99600.016.17446514-15
Produced by FastQC (version 0.11.5)