##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938952_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641092 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1313755825242 33.0 31.0 34.0 30.0 34.0 2 32.36406607569899 34.0 31.0 34.0 30.0 34.0 3 32.45949894967688 34.0 31.0 34.0 30.0 34.0 4 36.04399657414433 37.0 35.0 37.0 35.0 37.0 5 35.95579442139842 37.0 35.0 37.0 35.0 37.0 6 35.99079395946828 37.0 35.0 37.0 35.0 37.0 7 35.93490609187412 37.0 35.0 37.0 35.0 37.0 8 35.89386662789482 37.0 35.0 37.0 35.0 37.0 9 37.57414660299603 39.0 37.0 39.0 35.0 39.0 10-11 37.48894529232605 39.0 37.0 39.0 34.5 39.0 12-13 37.412993186151795 39.0 37.0 39.0 34.0 39.0 14-15 38.664550307221404 40.0 38.0 41.0 34.0 41.0 16-17 38.655758678607185 40.0 38.0 41.0 34.0 41.0 18-19 38.75313128054608 40.0 38.0 41.0 34.0 41.0 20-21 38.74654063546442 40.0 38.0 41.0 34.0 41.0 22-23 38.68662716028067 40.0 38.0 41.0 34.0 41.0 24-25 38.63191456412726 40.0 38.0 41.0 34.0 41.0 26-27 38.480115800585516 40.0 38.0 41.0 34.0 41.0 28-29 38.29652488440388 40.0 38.0 41.0 34.0 41.0 30-31 38.12859037095262 40.0 37.5 41.0 33.0 41.0 32-33 37.97812722919156 40.0 37.0 41.0 33.0 41.0 34-35 37.953457509242384 40.0 37.0 41.0 33.0 41.0 36-37 37.86298849112413 40.0 37.0 41.0 33.0 41.0 38-39 37.739135743851406 40.0 37.0 41.0 33.0 41.0 40-41 37.579897633251704 40.0 37.0 41.0 32.5 41.0 42-43 37.415954082629455 40.0 37.0 41.0 32.0 41.0 44-45 37.1709654567126 40.0 36.5 41.0 31.0 41.0 46-47 36.873829461450036 40.0 36.0 41.0 31.0 41.0 48-49 36.59097751990465 39.5 36.0 41.0 30.0 41.0 50-51 36.31947315731523 39.0 35.5 41.0 30.0 41.0 52-53 36.08274039677528 39.0 35.0 40.5 29.0 41.0 54-55 35.83080066881426 39.0 35.0 40.0 28.5 41.0 56-57 35.545559374682895 38.0 35.0 40.0 28.0 41.0 58-59 35.230080398562414 38.0 34.0 40.0 27.5 41.0 60-61 34.81574345005778 38.0 34.0 40.0 26.5 41.0 62-63 34.486260796670464 37.0 34.0 40.0 26.0 41.0 64-65 34.21828868513478 37.0 33.5 39.5 26.0 41.0 66-67 33.752779153471366 36.0 33.0 39.0 25.5 40.5 68-69 33.3259061024758 36.0 32.5 39.0 25.0 40.0 70-71 32.85147734346248 35.0 32.0 38.0 23.5 40.0 72-73 32.337862899134144 35.0 31.5 37.5 22.0 39.5 74-75 31.86267422717573 35.0 31.0 37.0 21.5 39.0 76-77 29.909092148247773 33.0 28.5 35.0 19.0 37.5 78-79 30.882106151546406 34.0 30.5 36.0 20.0 38.0 80-81 30.88073172006125 34.0 31.0 35.5 19.5 37.0 82-83 30.550008292024664 34.0 30.5 35.0 18.0 37.0 84-85 30.134579560272797 34.0 30.0 35.0 15.0 36.5 86-87 29.54106823995529 34.0 29.5 35.0 7.5 36.0 88-89 28.95429523091206 34.0 29.0 35.0 2.0 35.5 90-91 28.476644130533884 33.5 29.0 35.0 2.0 35.0 92-93 28.097432236362838 33.0 28.0 35.0 2.0 35.0 94-95 27.664431795636048 33.0 27.0 35.0 2.0 35.0 96-97 27.0054765225899 33.0 26.0 35.0 2.0 35.0 98-99 26.04259999273028 32.5 24.5 34.0 2.0 35.0 100-101 21.61814507029668 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12062520897623585 1101 2 0.11748376718126963 1101 3 0.144275471591925 1101 4 0.08254778170965693 1101 5 0.1147401970888069 1101 6 0.1338680565726662 1101 7 0.18243174963905062 1101 8 0.14866201782472643 1101 9 0.18415476672876707 1101 10-11 0.23751753272372156 1101 12-13 0.24221514635587482 1101 14-15 0.20214254691104827 1101 16-17 0.17120814667996598 1101 18-19 0.2957689584199841 1101 20-21 0.28837295230246696 1101 22-23 0.26559076206415 1101 24-25 0.2722030812032159 1101 26-27 0.350641596195608 1101 28-29 0.433373732717655 1101 30-31 0.31254180213024796 1101 32-33 0.3697296479738341 1101 34-35 0.38025844770483275 1101 36-37 0.430320012066538 1101 38-39 0.4752497669807241 1101 40-41 0.5165748056651438 1101 42-43 0.3478931504372085 1101 44-45 0.3809832755659315 1101 46-47 0.3776564946876917 1101 48-49 0.24462885460436468 1101 50-51 0.23341569544643903 1101 52-53 0.26180540382148365 1101 54-55 0.27440394728643724 1101 56-57 0.14670885787680987 1101 58-59 0.2347457704264002 1101 60-61 0.3934234974970465 1101 62-63 0.1846873295618252 1101 64-65 0.26325010490705836 1101 66-67 0.2490973404113177 1101 68-69 0.2457741081431024 1101 70-71 0.3165730463928398 1101 72-73 0.22628155544244777 1101 74-75 0.2215166291000692 1101 76-77 0.14368096985941392 1101 78-79 0.3602336655891776 1101 80-81 0.5556018522423649 1101 82-83 0.4386362186917019 1101 84-85 0.45457582547847863 1101 86-87 0.6184767192424587 1101 88-89 0.7051257850239487 1101 90-91 0.6368410191567726 1101 92-93 0.47868596526388885 1101 94-95 0.4462079566082089 1101 96-97 0.3217010898213353 1101 98-99 0.2855467994181744 1101 100-101 0.9692497904846178 1102 1 0.09092436076416277 1102 2 0.033687542659997405 1102 3 0.07901716408428427 1102 4 0.07786926958903706 1102 5 0.06589168453664485 1102 6 0.11177090935904488 1102 7 0.11197308858984201 1102 8 0.08240046419173552 1102 9 0.1646139232476287 1102 10-11 0.28912045695362565 1102 12-13 0.15789939793299368 1102 14-15 0.26162863300449857 1102 16-17 0.19719968533698307 1102 18-19 0.12326528635056633 1102 20-21 0.16034759064957882 1102 22-23 0.26386604819787607 1102 24-25 0.1743930570530665 1102 26-27 0.1421756980929345 1102 28-29 0.2071201545477308 1102 30-31 0.19417530163237728 1102 32-33 0.24114808168239676 1102 34-35 0.35424533214372644 1102 36-37 0.15165605396729376 1102 38-39 0.2301458062602748 1102 40-41 0.18010999759570012 1102 42-43 0.11595253966697072 1102 44-45 0.040639099971492954 1102 46-47 0.17419084431985254 1102 48-49 0.08584026191397953 1102 50-51 0.14995663003006143 1102 52-53 0.14442768493178448 1102 54-55 0.0989152304089842 1102 56-57 0.0872456273188007 1102 58-59 0.0020323069177408115 1102 60-61 0.03487728536251211 1102 62-63 0.10849504578237656 1102 64-65 0.15575460808685904 1102 66-67 0.12676676306130474 1102 68-69 0.2717591737289027 1102 70-71 0.3169404131634508 1102 72-73 0.3281450807249797 1102 74-75 0.33504830479440173 1102 76-77 0.1663602939109481 1102 78-79 0.21848834977522458 1102 80-81 0.37600976760975513 1102 82-83 0.2333584280388159 1102 84-85 0.17287160167963123 1102 86-87 0.4005231367571511 1102 88-89 0.35918991477945994 1102 90-91 0.3783273322072809 1102 92-93 0.5870109741743512 1102 94-95 0.5143814636862309 1102 96-97 0.3425013851851766 1102 98-99 0.37230665167232146 1102 100-101 0.8100030481975651 1103 1 0.07584912326329629 1103 2 0.0910899419388258 1103 3 0.096207372807676 1103 4 0.08342020225666857 1103 5 0.05340988715781947 1103 6 0.0015497041721630467 1103 7 0.05425864437653871 1103 8 0.08796179835719187 1103 9 0.1126688431560936 1103 10-11 0.19104126688358747 1103 12-13 0.1437186892442739 1103 14-15 0.20772113699173644 1103 16-17 0.16019462327471956 1103 18-19 0.18348475795447428 1103 20-21 0.12378632582745297 1103 22-23 0.19003448311917026 1103 24-25 0.10206277482576098 1103 26-27 0.0165412067261812 1103 28-29 0.10181539066172718 1103 30-31 0.11853907668265151 1103 32-33 0.18384449663281544 1103 34-35 0.10084057519205913 1103 36-37 -0.012611333869891439 1103 38-39 0.13136104792174308 1103 40-41 0.12851163409802524 1103 42-43 0.19792154320348487 1103 44-45 0.24317879902148576 1103 46-47 0.20927683003512243 1103 48-49 0.12925958096258938 1103 50-51 0.15101411564132405 1103 52-53 0.1864514696901196 1103 54-55 0.2010770083716764 1103 56-57 0.19397886357148764 1103 58-59 0.04628374579160521 1103 60-61 0.08925111519400986 1103 62-63 0.2085210495269223 1103 64-65 0.1251325985174745 1103 66-67 0.07611493586485807 1103 68-69 0.09830899690343387 1103 70-71 -0.08398705372511728 1103 72-73 0.007759878589212121 1103 74-75 -0.042101338087501716 1103 76-77 -0.028702461792537548 1103 78-79 0.22762259844036592 1103 80-81 0.26684951520007516 1103 82-83 0.34552471331722856 1103 84-85 0.306922638362245 1103 86-87 0.18801706921675532 1103 88-89 0.2302130754479279 1103 90-91 0.3414886941100619 1103 92-93 0.3404243990408631 1103 94-95 0.25166043185764764 1103 96-97 0.12133780429619634 1103 98-99 0.1139386466796033 1103 100-101 0.6784414366588365 1104 1 0.05511296718879777 1104 2 0.0174649453814979 1104 3 0.02253730853610847 1104 4 0.06319481361023094 1104 5 0.06645323478763032 1104 6 0.11290952229889939 1104 7 0.007567866482752095 1104 8 0.01962286911600586 1104 9 0.11568086872451033 1104 10-11 0.09727100206069395 1104 12-13 0.12058267738645156 1104 14-15 0.10600925238512104 1104 16-17 0.06799953765857936 1104 18-19 0.10521997762202773 1104 20-21 0.07042896889866768 1104 22-23 0.07157712594000998 1104 24-25 0.14621192141851225 1104 26-27 0.11161061100610681 1104 28-29 0.07277018774434652 1104 30-31 0.11710097844004252 1104 32-33 0.14843566987060086 1104 34-35 0.08046026627791747 1104 36-37 0.02848791775567605 1104 38-39 0.24635351159841434 1104 40-41 0.08517558418303395 1104 42-43 0.1886404108020514 1104 44-45 0.23590005020138705 1104 46-47 0.22044955455370996 1104 48-49 0.051889895510193185 1104 50-51 0.12145407668278807 1104 52-53 0.05579985313625002 1104 54-55 0.16529543405626868 1104 56-57 0.13629719686726816 1104 58-59 0.2630625809914946 1104 60-61 0.2044817492216353 1104 62-63 0.2963505132962325 1104 64-65 0.1677140771891814 1104 66-67 0.2106583373255546 1104 68-69 0.2888534148914701 1104 70-71 0.20796848447102434 1104 72-73 0.058262633979182965 1104 74-75 0.07398871221307601 1104 76-77 0.0049130455071804136 1104 78-79 0.14043503662874102 1104 80-81 0.28080016354921966 1104 82-83 0.26806238867445487 1104 84-85 0.16310279905368574 1104 86-87 0.08450654465359975 1104 88-89 0.29280148954010343 1104 90-91 0.49408734858841896 1104 92-93 0.47255229620635575 1104 94-95 0.4671836304635555 1104 96-97 0.48719969929343065 1104 98-99 0.40303958333693046 1104 100-101 1.0687838636553977 1105 1 -0.10955019152198275 1105 2 -0.057078511602433935 1105 3 -0.09773312869861428 1105 4 -0.0649679187191694 1105 5 -0.03285849845266853 1105 6 -0.03415086880911389 1105 7 -0.1054377346180857 1105 8 -0.03289774739270257 1105 9 -0.13713179480243554 1105 10-11 -0.13289620727317697 1105 12-13 -0.09497582165418805 1105 14-15 -0.08853270257350232 1105 16-17 -0.050325231707859075 1105 18-19 -0.10842465045712402 1105 20-21 -0.04286280436334522 1105 22-23 -0.09246614450207602 1105 24-25 -0.1612538711663305 1105 26-27 -0.07009175298792059 1105 28-29 -0.037908788262008386 1105 30-31 -0.05833012705058849 1105 32-33 -0.12200641009528113 1105 34-35 -0.035618127330231175 1105 36-37 -0.010522647296554055 1105 38-39 -0.11696006525682634 1105 40-41 -0.01282036277125087 1105 42-43 -0.015007269780149102 1105 44-45 -0.05751453785605776 1105 46-47 -0.07392802553815159 1105 48-49 -0.1091045058115867 1105 50-51 -0.16984616848650091 1105 52-53 0.16714565570229212 1105 54-55 0.08394350225790959 1105 56-57 0.0174576204383996 1105 58-59 -0.04208809395085211 1105 60-61 -0.13799576885111975 1105 62-63 -0.21637945404683023 1105 64-65 -0.14900128416903868 1105 66-67 0.047802757059265844 1105 68-69 0.08814891656792412 1105 70-71 0.1157026429492305 1105 72-73 -0.04207417097071442 1105 74-75 0.012171285812318189 1105 76-77 -0.13453970852890862 1105 78-79 -0.0022667556266178224 1105 80-81 -0.10066702446558295 1105 82-83 0.10558049776994594 1105 84-85 -0.1356897058400115 1105 86-87 -0.16382093804888243 1105 88-89 0.08422468274901007 1105 90-91 0.1716294658626758 1105 92-93 0.11700231268018157 1105 94-95 0.005719664535302371 1105 96-97 -0.10732584240473031 1105 98-99 0.0799769537266819 1105 100-101 0.5427524931299779 1106 1 -0.009340654768102752 1106 2 -0.0863605650626198 1106 3 -0.0751689934624622 1106 4 -0.025885066754057107 1106 5 -0.01542305842549041 1106 6 -0.0243252939988281 1106 7 -0.09976042121905948 1106 8 -0.026466526416378144 1106 9 -0.03965473058225655 1106 10-11 0.010268364025947108 1106 12-13 0.04988592656192026 1106 14-15 -0.013718878810905721 1106 16-17 -0.05032346197175741 1106 18-19 -0.09150774310634802 1106 20-21 -0.12954978760041058 1106 22-23 0.058062298696476944 1106 24-25 0.030953626851569993 1106 26-27 0.010153390856224576 1106 28-29 0.04253810549719361 1106 30-31 0.0527349709220033 1106 32-33 -0.0169194806582027 1106 34-35 0.1653442551592903 1106 36-37 0.10349967969404616 1106 38-39 0.1487357241870484 1106 40-41 0.14961840435979212 1106 42-43 0.24299016764198456 1106 44-45 0.17619681110758734 1106 46-47 0.16337234823011215 1106 48-49 0.0016898225381751786 1106 50-51 0.06888061257590294 1106 52-53 0.11669578878644415 1106 54-55 0.19105677952600075 1106 56-57 0.09224843763174562 1106 58-59 0.16893346851374957 1106 60-61 0.08601742630121834 1106 62-63 0.07401477236612664 1106 64-65 -0.05275595627232832 1106 66-67 0.21812746823832896 1106 68-69 0.061447138337086926 1106 70-71 0.12141712080629219 1106 72-73 0.25131444891828636 1106 74-75 0.05791896391855644 1106 76-77 0.047697096318753296 1106 78-79 0.2621158896333178 1106 80-81 0.34510826152376595 1106 82-83 0.3061287620127793 1106 84-85 0.23990315575246868 1106 86-87 0.14087983497184453 1106 88-89 0.3727907717738148 1106 90-91 0.4810979079285964 1106 92-93 0.4479500417548188 1106 94-95 0.6340432289253819 1106 96-97 0.4720769070572679 1106 98-99 0.45539461144555915 1106 100-101 0.5278106112634049 1107 1 -0.14923609185320785 1107 2 -0.12610892412877917 1107 3 -0.13544767251072187 1107 4 -0.16659863988748924 1107 5 -0.21839655174986916 1107 6 -0.0962932957889393 1107 7 -0.17550793184783942 1107 8 -0.07300238455018615 1107 9 -0.1552974452089444 1107 10-11 -0.09363374867637475 1107 12-13 -0.12249953439130934 1107 14-15 -0.0308152417183436 1107 16-17 -0.12292196461963556 1107 18-19 -0.15129577361280155 1107 20-21 -0.15529834712155832 1107 22-23 -0.13329063260806606 1107 24-25 -0.1687158555083883 1107 26-27 -0.249184082105657 1107 28-29 -0.2422664741999796 1107 30-31 -0.20001137893255105 1107 32-33 -0.19395386568095319 1107 34-35 -0.23717168800472876 1107 36-37 -0.1497338223944169 1107 38-39 -0.22890865002045757 1107 40-41 -0.22277218451169034 1107 42-43 -0.33535976149951807 1107 44-45 -0.3417427447214578 1107 46-47 -0.4872142156917221 1107 48-49 -0.46424273601333255 1107 50-51 -0.32437652455395494 1107 52-53 -0.2748522364988517 1107 54-55 -0.4186818364954732 1107 56-57 -0.3373936103655737 1107 58-59 -0.32783128124383865 1107 60-61 -0.4768456070022822 1107 62-63 -0.5375576571483052 1107 64-65 -0.5034714174120367 1107 66-67 -0.3821670374303494 1107 68-69 -0.35924785774704304 1107 70-71 -0.4687363438017442 1107 72-73 -0.37140362745972766 1107 74-75 -0.49472610955052687 1107 76-77 -0.7213123297273434 1107 78-79 -0.5107144182282433 1107 80-81 -0.4521542459350236 1107 82-83 -0.40540311033002396 1107 84-85 -0.45642094266627 1107 86-87 -0.3863960283831176 1107 88-89 -0.1822189712708635 1107 90-91 -0.2223901253749041 1107 92-93 -0.08834105151735017 1107 94-95 -0.08056364621446832 1107 96-97 -0.0026039965751643024 1107 98-99 -0.21469144434619025 1107 100-101 0.308078731461805 1108 1 0.05785453204216395 1108 2 0.05430143718693614 1108 3 -0.021516966884625788 1108 4 -0.05532875056550779 1108 5 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-3.6777552608552284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 14.0 5 42.0 6 119.0 7 294.0 8 738.0 9 1346.0 10 2339.0 11 3585.0 12 5199.0 13 6988.0 14 8634.0 15 11749.0 16 15275.0 17 21170.0 18 20848.0 19 14847.0 20 14259.0 21 15137.0 22 16471.0 23 18648.0 24 21497.0 25 25540.0 26 30875.0 27 37577.0 28 45815.0 29 55415.0 30 67370.0 31 81931.0 32 101507.0 33 129021.0 34 172356.0 35 244861.0 36 382626.0 37 591879.0 38 442159.0 39 32952.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.100189199042184 19.814807704662464 15.13520109573502 22.94980200056034 2 15.76559998667218 20.92842657506819 39.531830015766204 23.774143422493424 3 19.423291577877634 24.48695463846015 30.21897760471805 25.870776178944165 4 13.382494816538006 17.427677642429725 36.75248722876749 32.43734031226477 5 13.846143360940705 37.2792666036117 34.35073201059263 14.523858024854967 6 31.817596660775166 36.32664064712626 17.299927454249982 14.555835237848585 7 28.20647671493458 32.87272840173686 20.76440351188069 18.15639137144787 8 25.910494598446398 35.62935331294783 19.580764320213003 18.87938776839277 9 25.932190169823695 17.13639661170455 20.419508294296453 36.511904924175305 10-11 24.981200957785642 26.793689882821198 27.087621332388267 21.137487827004893 12-13 25.944249575554355 24.012681118264716 27.667760153754585 22.375309152426347 14-15 22.801955432910827 25.426591373437557 26.078187782747175 25.69326541090444 16-17 22.120506644296558 28.576713317123716 26.865237334981177 22.43754270359855 18-19 22.0622378925081 27.320555285465254 28.893124510619096 21.724082311407557 20-21 22.97827943895934 26.400935673577447 28.892726947792806 21.728057939670407 22-23 22.52555003763595 26.333974734693072 28.842444716049272 22.298030511621707 24-25 22.51065089743182 26.447355866437068 28.94092670758913 22.10106652854198 26-27 22.317227116662348 26.573742982069536 28.959063145093015 22.149966756175097 28-29 22.152636106580157 26.94947771603564 28.874382263094205 22.02350391429 30-31 22.40807968825016 26.566302877749052 28.780765683285548 22.244851750715235 32-33 22.269500645944934 26.581012702321615 28.81586480137761 22.333621850355836 34-35 22.596243318178246 26.666325890207503 28.673972535310593 22.063458256303655 36-37 22.571931055544187 26.400201145110934 28.82117491540224 22.20669288394264 38-39 22.33591499360502 26.775726902566227 28.766701032001418 22.121657071827336 40-41 22.57140693480751 26.617998693689003 28.689296769246788 22.1212976022567 42-43 22.298598979051704 26.667162677001855 28.50760713255289 22.526631211393546 44-45 22.491638368600135 26.774296817421195 28.345887849352625 22.388176964626044 46-47 22.763361559117392 26.90531615048972 27.939531646854093 22.39179064353879 48-49 22.692069372286035 26.939909321306953 27.943228602957838 22.424792703449175 50-51 22.52656162233908 27.167152169636953 27.94020844917156 22.366077758852406 52-53 22.751630764850297 27.192540055401327 27.574578999898524 22.481250179849848 54-55 22.61305172254507 27.039970587923477 27.672114413280568 22.674863276250885 56-57 22.625811596112516 27.34262948810568 27.494006267104666 22.53755264867714 58-59 22.660987935630953 27.044473801088415 27.671867942052558 22.62267032122807 60-61 22.595020656705657 27.06329459972261 27.94952047136137 22.392164272210362 62-63 22.506504885100558 27.01647651804632 27.96138869831115 22.515629898541967 64-65 22.43241984547266 27.12914308735119 27.836598278132556 22.601838789043597 66-67 22.41111619958225 27.191228931410333 27.552752289670025 22.844902579337393 68-69 22.42559324629819 27.02368630848334 27.800387763837776 22.75033268138069 70-71 22.525871874209606 26.922670622606105 27.610700422401035 22.940757080783253 72-73 22.487384006312542 26.870627755489018 27.664276746133798 22.977711492064646 74-75 22.26014845374565 27.307965795966215 27.353950562873237 23.0779351874149 76-77 22.38527394047079 27.00380968591652 27.553398437344512 23.057517936268177 78-79 22.36869381696221 27.070126578492165 27.564430347563135 22.99674925698249 80-81 22.316185880688746 27.0335717195766 27.495236818709834 23.155005581024817 82-83 22.353064565717514 26.945842855909603 27.615679423511185 23.0854131548617 84-85 22.196311222782093 27.002807929447364 27.508280665724634 23.292600182045913 86-87 22.16415666476696 26.88528841986479 27.663352986388468 23.28720192897978 88-89 22.186768351941385 26.824346281105356 27.444680204970318 23.544205161982948 90-91 22.10108979294333 26.837924999586832 27.545685383811552 23.515299823658285 92-93 22.15390452131164 26.81033072683572 27.485676379315827 23.550088372536816 94-95 22.02674121158975 26.72252613691609 27.537113436411907 23.713619215082247 96-97 21.855006987674354 26.54939515251153 27.745061772101476 23.850536087712634 98-99 21.722529923228727 26.70115240211246 27.50727729287734 24.069040381781477 100-101 21.876030588920393 26.09298747068915 28.039704863944277 23.991277076446174 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 560.0 1 414.5 2 302.5 3 358.0 4 333.5 5 334.0 6 588.0 7 1316.5 8 2113.0 9 2569.5 10 2486.5 11 2189.5 12 2235.5 13 2431.5 14 2689.0 15 2920.0 16 3072.5 17 3282.0 18 3605.0 19 3911.5 20 4407.5 21 5208.0 22 6248.5 23 7566.5 24 9193.5 25 11409.5 26 14795.0 27 18706.0 28 22049.0 29 25669.0 30 29496.5 31 34374.5 32 39451.0 33 43988.5 34 49081.0 35 53923.5 36 59674.0 37 64954.5 38 68841.5 39 71849.5 40 74596.0 41 78623.5 42 81834.5 43 84827.0 44 89000.5 45 92793.0 46 95377.0 47 100322.0 48 109600.0 49 110404.5 50 103365.0 51 99613.5 52 97804.5 53 96515.0 54 93494.0 55 86305.5 56 78091.5 57 70601.5 58 63668.0 59 57033.5 60 48388.5 61 39277.0 62 30812.5 63 22785.5 64 16422.5 65 11893.5 66 8542.0 67 6389.5 68 4666.0 69 3296.5 70 2354.0 71 1437.0 72 929.0 73 639.5 74 414.0 75 275.0 76 162.0 77 90.0 78 51.5 79 27.5 80 16.5 81 10.5 82 8.0 83 5.0 84 1.5 85 2.5 86 2.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39866085694856523 2 0.0 3 0.0 4 0.0 5 3.7863126312903905E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0015145250525161562 16-17 0.0025557610261210135 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024421716471823016 36-37 0.008045914341492079 38-39 0.013233162646359913 40-41 0.0025368294629645616 42-43 6.815362736322703E-4 44-45 3.9756282628549105E-4 46-47 3.029050105032312E-4 48-49 3.2183657365968316E-4 50-51 6.247415841629145E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.4076813681613516E-4 60-61 0.0023096507050871383 62-63 0.0 64-65 2.2717875787742344E-4 66-67 0.0017227722472371275 68-69 0.0019499510051145513 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12155956702757799 78-79 1.3252094209516367E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1455079944204897 88-89 0.43695940921406756 90-91 0.3402569846109109 92-93 0.0 94-95 0.0 96-97 4.922206420677508E-4 98-99 0.0 100-101 1.8931563156451953E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641092.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.838137845994886 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9828703882832 41.86903852829325 2 13.832848901246745 15.44801047501269 3 4.773156759899269 7.99572555359393 4 2.157660897615336 4.819190665038327 5 1.1587995166900842 3.2352603574406587 6 0.7001963133828473 2.3458594959557315 7 0.47033529723943485 1.8383853012764777 8 0.33681107341611677 1.5045522516373304 9 0.24671013539911912 1.2398251093578068 >10 1.2368105372544291 12.218040571968846 >50 0.07198292879758642 2.7287646695672385 >100 0.029783288908052874 2.9490433966685012 >500 0.0012881758496374198 0.490936678787236 >1k 6.101885603325179E-4 0.7134254866974731 >5k 1.3559745785167064E-4 0.6039414587044196 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8192 0.31017473075530877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7761 0.2938557233144472 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4575 0.17322380288153535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4074 0.1542543765987705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.78631263129039E-5 0.0 0.0 0.0 6 0.0 3.78631263129039E-5 0.0 0.0 0.0 7 0.0 3.78631263129039E-5 0.0 3.78631263129039E-5 0.0 8 0.0 3.78631263129039E-5 0.0 3.78631263129039E-5 0.0 9 0.0 3.78631263129039E-5 0.0 3.78631263129039E-5 0.0 10-11 0.0 3.78631263129039E-5 0.0 7.57262526258078E-5 0.0 12-13 0.0 3.78631263129039E-5 0.0 7.57262526258078E-5 5.6794689469355854E-5 14-15 0.0 3.78631263129039E-5 0.0 7.57262526258078E-5 7.57262526258078E-5 16-17 0.0 3.78631263129039E-5 0.0 9.465781578225976E-5 7.57262526258078E-5 18-19 0.0 3.78631263129039E-5 0.0 1.3252094209516367E-4 7.57262526258078E-5 20-21 0.0 3.78631263129039E-5 0.0 2.082471947209715E-4 7.57262526258078E-5 22-23 0.0 3.78631263129039E-5 0.0 5.111522052242027E-4 7.57262526258078E-5 24-25 3.78631263129039E-5 3.78631263129039E-5 0.0 7.7619408941453E-4 7.57262526258078E-5 26-27 3.78631263129039E-5 3.78631263129039E-5 0.0 0.0011737569157000212 7.57262526258078E-5 28-29 3.78631263129039E-5 3.78631263129039E-5 0.0 0.002801871347154889 7.57262526258078E-5 30-31 3.78631263129039E-5 3.78631263129039E-5 0.0 0.007383309631016261 7.57262526258078E-5 32-33 3.78631263129039E-5 3.78631263129039E-5 0.0 0.01771994311443903 7.57262526258078E-5 34-35 5.6794689469355854E-5 3.78631263129039E-5 0.0 0.03402001899214416 1.1358937893871172E-4 36-37 7.57262526258078E-5 3.78631263129039E-5 0.0 0.06097856492693174 1.3252094209516367E-4 38-39 7.57262526258078E-5 3.78631263129039E-5 0.0 0.10221150948168409 1.514525052516156E-4 40-41 7.57262526258078E-5 3.78631263129039E-5 0.0 0.1475904663676994 1.514525052516156E-4 42-43 7.57262526258078E-5 3.78631263129039E-5 0.0 0.19274224449583732 1.514525052516156E-4 44-45 9.465781578225976E-5 3.78631263129039E-5 0.0 0.251373295591369 1.7038406840806755E-4 46-47 1.1358937893871172E-4 3.78631263129039E-5 0.0 0.32384331935426713 2.461103210338754E-4 48-49 1.1358937893871172E-4 3.78631263129039E-5 0.0 0.402882595532454 3.029050105032312E-4 50-51 1.1358937893871172E-4 3.78631263129039E-5 0.0 0.49110367984152004 3.029050105032312E-4 52-53 1.1358937893871172E-4 3.78631263129039E-5 0.0 0.5763714402981797 3.407681368161351E-4 54-55 1.1358937893871172E-4 3.78631263129039E-5 0.0 0.6727898914539895 3.7863126312903905E-4 56-57 1.514525052516156E-4 3.78631263129039E-5 0.0 0.7886510579714754 3.7863126312903905E-4 58-59 1.514525052516156E-4 3.78631263129039E-5 0.0 0.920452600666694 3.7863126312903905E-4 60-61 1.514525052516156E-4 3.78631263129039E-5 0.0 1.0650329484925174 3.7863126312903905E-4 62-63 1.514525052516156E-4 3.78631263129039E-5 0.0 1.2146869552442703 3.7863126312903905E-4 64-65 1.8931563156451953E-4 3.78631263129039E-5 0.0 1.373390249184807 3.7863126312903905E-4 66-67 1.8931563156451953E-4 3.78631263129039E-5 0.0 1.564864078949162 3.7863126312903905E-4 68-69 1.8931563156451953E-4 3.78631263129039E-5 0.0 1.7814600930221287 3.7863126312903905E-4 70-71 1.8931563156451953E-4 3.78631263129039E-5 0.0 2.0193162525197907 3.7863126312903905E-4 72-73 1.8931563156451953E-4 3.78631263129039E-5 0.0 2.2661838360799242 3.7863126312903905E-4 74-75 1.8931563156451953E-4 3.78631263129039E-5 0.0 2.5259248825864455 3.7863126312903905E-4 76-77 1.8931563156451953E-4 3.78631263129039E-5 0.0 2.8121890490751555 3.97562826285491E-4 78-79 1.8931563156451953E-4 3.78631263129039E-5 0.0 3.136960015024089 4.1649438944194294E-4 80-81 2.2717875787742344E-4 3.78631263129039E-5 0.0 3.4975494984650286 4.1649438944194294E-4 82-83 2.2717875787742344E-4 3.78631263129039E-5 0.0 3.8700847982576905 4.1649438944194294E-4 84-85 2.461103210338754E-4 3.78631263129039E-5 0.0 4.250249518002402 4.1649438944194294E-4 86-87 2.6504188419032733E-4 3.78631263129039E-5 0.0 4.649232211524627 4.1649438944194294E-4 88-89 2.6504188419032733E-4 3.78631263129039E-5 0.0 5.0959413757642675 4.1649438944194294E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11690 0.0 26.84943 1 GTATCAA 15570 0.0 24.074114 1 GAGTACT 8280 0.0 22.264359 12-13 GTACATG 9690 0.0 21.577688 1 GTACTTT 8680 0.0 20.828346 14-15 TACATGG 9670 0.0 20.65724 2 TATCAAC 18100 0.0 20.499573 2 ATCAACG 18105 0.0 20.467705 3 TCAACGC 18105 0.0 20.467705 4 CAACGCA 18130 0.0 20.439482 5 AACGCAG 18530 0.0 20.049093 6 ACATGGG 9615 0.0 19.689753 3 AGTACTT 8805 0.0 18.700346 12-13 AGAGTAC 14520 0.0 18.366196 10-11 ACGCAGA 20335 0.0 17.966146 7 CGCAGAG 20275 0.0 17.8321 8 CATGGGG 5075 0.0 17.763733 4 ACTTTTT 10550 0.0 17.247635 16-17 GCAGAGT 21140 0.0 16.340279 9 TACTTTT 9960 0.0 16.174465 14-15 >>END_MODULE