FastQCFastQC Report
Fri 27 May 2016
SRR938951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010587
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98970.49224430477268577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64350.32005578470367113No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58130.2891195456849169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53740.26728512618454214No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42700.21237578876218735No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36830.18318033489722158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.16298722711327587No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29540.14692226697974273No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24370.12120838342235378No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24090.11981575529932302No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23940.11906970451912799No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21730.10807788969092111No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20900.10394974204050857No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20220.10056764517029106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT4650.023.4281023
GTACATG69900.023.019971
TACATGG68800.022.718922
GAGTACT57700.021.04594212-13
ACATGGG72750.020.899423
GTACTTT61600.019.25331114-15
AGTACTT60600.019.10130712-13
AGAGTAC98350.018.98972510-11
GTATAGG4900.018.4051671
TATAGCG1600.00121430817.7620645
CATGGGA33300.017.3531284
ACTTTTT73350.016.91350616-17
TACTTTT68700.016.67773414-15
AGTACAT54150.016.6194762
CATGGGG40750.016.5052814
CTTTGCG6650.016.3820572
GAGTACA63150.016.1602231
GTATTAT5900.015.2856471
ACTATAC5307.2759576E-1215.1927113
GTATATG6900.015.1340721
Produced by FastQC (version 0.11.5)