##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938951_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2010587 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.568605088961583 33.0 31.0 34.0 30.0 34.0 2 31.655189255675083 33.0 31.0 34.0 30.0 34.0 3 31.694425558307103 33.0 31.0 34.0 28.0 34.0 4 35.23695517776649 37.0 35.0 37.0 33.0 37.0 5 35.27626857231246 37.0 35.0 37.0 33.0 37.0 6 35.35404735035092 37.0 35.0 37.0 33.0 37.0 7 35.26186680805158 37.0 35.0 37.0 32.0 37.0 8 35.24515377847365 37.0 35.0 37.0 32.0 37.0 9 36.8113531023527 39.0 37.0 39.0 33.0 39.0 10-11 36.68243080254672 39.0 37.0 39.0 32.0 39.0 12-13 36.56555349258699 39.0 37.0 39.0 32.0 39.0 14-15 37.7108722477565 40.0 37.5 41.0 32.0 41.0 16-17 37.620775673969845 40.0 37.0 41.0 32.0 41.0 18-19 37.52285601170205 40.0 37.0 41.0 32.0 41.0 20-21 37.63198284878993 40.0 37.0 41.0 32.0 41.0 22-23 37.56310420787561 40.0 37.0 41.0 32.0 41.0 24-25 37.46859723056003 40.0 37.0 41.0 31.5 41.0 26-27 37.3355823448575 40.0 37.0 41.0 31.0 41.0 28-29 37.19521338793099 40.0 37.0 41.0 31.0 41.0 30-31 37.00378819717824 40.0 36.5 41.0 30.0 41.0 32-33 36.79333746811255 39.5 36.0 41.0 30.0 41.0 34-35 36.447367360875205 39.0 35.5 41.0 30.0 41.0 36-37 36.16607438524172 39.0 35.0 41.0 29.5 41.0 38-39 35.92247935553149 39.0 35.0 40.5 28.0 41.0 40-41 35.771801966291434 39.0 35.0 40.0 27.5 41.0 42-43 35.550514600959815 39.0 35.0 40.0 27.0 41.0 44-45 35.49426709712139 39.0 35.0 40.5 26.5 41.0 46-47 35.28058298397433 39.0 35.0 41.0 25.5 41.0 48-49 35.02551991035454 39.0 34.5 40.5 24.0 41.0 50-51 33.90588793223074 38.0 33.0 39.5 23.0 40.5 52-53 34.015404953876654 38.0 33.5 39.5 23.0 40.5 54-55 34.49352552264587 38.0 34.0 40.0 23.0 41.0 56-57 34.364751686945155 38.0 34.0 40.0 23.0 41.0 58-59 34.13050218667483 38.0 34.0 40.0 22.5 41.0 60-61 33.77535291932157 37.5 33.0 40.0 22.0 41.0 62-63 33.375054896903244 37.0 33.0 40.0 20.5 41.0 64-65 32.963953810504094 36.0 32.5 39.5 19.5 41.0 66-67 32.5178629425138 36.0 32.0 39.0 18.0 41.0 68-69 32.04591992288819 35.0 31.0 39.0 14.0 40.0 70-71 31.568893561929926 35.0 31.0 38.0 11.0 40.0 72-73 31.117095156787546 35.0 31.0 37.0 7.5 39.5 74-75 30.63978703731796 35.0 30.0 37.0 7.0 39.0 76-77 30.202133754968077 34.0 30.0 36.0 2.0 39.0 78-79 29.791368640103613 34.0 29.5 36.0 2.0 38.0 80-81 29.327349923181636 34.0 29.0 35.0 2.0 37.0 82-83 28.846137471295698 34.0 29.0 35.0 2.0 37.0 84-85 28.413829394102322 34.0 28.5 35.0 2.0 36.0 86-87 27.968695957946608 34.0 27.0 35.0 2.0 36.0 88-89 27.4547622162085 33.5 26.0 35.0 2.0 35.5 90-91 26.96585375315766 33.0 25.5 35.0 2.0 35.0 92-93 26.416047900439025 33.0 24.5 35.0 2.0 35.0 94-95 25.83550276610761 32.5 23.5 35.0 2.0 35.0 96-97 25.125905767818054 32.0 19.0 34.5 2.0 35.0 98-99 24.203228957513403 32.0 4.5 34.0 2.0 35.0 100-101 21.058279746163684 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3019177941488209 1101 2 0.4095965023336383 1101 3 0.5257651739915374 1101 4 0.3365653931382653 1101 5 0.25069952025427256 1101 6 0.3084497872682661 1101 7 0.4594220363777808 1101 8 0.3849618978454785 1101 9 0.4862427927577144 1101 10-11 0.22248270365611234 1101 12-13 0.37017205781975093 1101 14-15 0.4958954415887149 1101 16-17 0.46028162520985916 1101 18-19 0.4991620241615635 1101 20-21 0.5813729150185196 1101 22-23 0.6882898308265695 1101 24-25 0.6642425054173415 1101 26-27 0.7430570730825323 1101 28-29 0.7232789330947398 1101 30-31 0.4895437390462476 1101 32-33 0.5225281347984136 1101 34-35 0.38846494105178664 1101 36-37 0.48045253939078947 1101 38-39 0.6035433464981068 1101 40-41 0.44671456460084613 1101 42-43 0.5083501841379459 1101 44-45 0.45225336561937723 1101 46-47 0.5402122220771659 1101 48-49 0.43590697355560337 1101 50-51 0.42295383586024826 1101 52-53 0.40527328631369386 1101 54-55 0.36121246353707903 1101 56-57 0.4875378459559414 1101 58-59 0.46995126722354286 1101 60-61 0.5919840049841838 1101 62-63 0.5695892286783675 1101 64-65 0.4649129838741928 1101 66-67 0.5646340177271654 1101 68-69 0.45993271078716447 1101 70-71 0.3781026311822586 1101 72-73 0.3089620556604551 1101 74-75 0.09992674186456085 1101 76-77 0.1317556440801404 1101 78-79 0.08569636759506949 1101 80-81 0.08818069768335235 1101 82-83 0.1183284157945117 1101 84-85 0.042944140924326746 1101 86-87 0.06301175259157432 1101 88-89 -0.11567770776443709 1101 90-91 -0.053181127097513325 1101 92-93 -0.06592488901113924 1101 94-95 -0.2744130025579281 1101 96-97 -0.4876798188939091 1101 98-99 -0.45661949910684285 1101 100-101 -0.015311049775711183 1102 1 0.3140868372001755 1102 2 0.27820223993433046 1102 3 0.428898795181631 1102 4 0.21105413410587914 1102 5 0.21511511759389634 1102 6 0.3483371140151732 1102 7 0.4132906874500577 1102 8 0.4653668138933966 1102 9 0.35689633079737604 1102 10-11 0.5192846740558821 1102 12-13 0.5175849406074988 1102 14-15 0.6931156520079469 1102 16-17 0.8143006547160994 1102 18-19 0.7045864285910923 1102 20-21 0.6489865419583793 1102 22-23 0.6998338635117634 1102 24-25 0.7038702635070919 1102 26-27 0.6811514687215592 1102 28-29 0.5224117692209447 1102 30-31 0.6491339578136959 1102 32-33 0.6418255026229573 1102 34-35 0.6731979364022109 1102 36-37 0.5306399595713529 1102 38-39 0.6193325292023815 1102 40-41 0.6452362119081343 1102 42-43 0.5470386298773207 1102 44-45 0.7767772133169828 1102 46-47 1.0192518456887925 1102 48-49 0.8909753349282568 1102 50-51 0.7875471782973804 1102 52-53 0.6158553393110324 1102 54-55 0.9084804965097604 1102 56-57 0.7240082097943485 1102 58-59 0.7053280702443843 1102 60-61 0.7444201909005841 1102 62-63 0.7296861453676087 1102 64-65 0.5489073557401554 1102 66-67 0.6573072155356243 1102 68-69 0.718549892628964 1102 70-71 0.7174542347581649 1102 72-73 0.5906639857013118 1102 74-75 0.6936915654739728 1102 76-77 0.5000890972002132 1102 78-79 0.6811634529624726 1102 80-81 0.7111197667745017 1102 82-83 0.7152088925826057 1102 84-85 0.6833367834382997 1102 86-87 0.7829791890262783 1102 88-89 0.4344884272333793 1102 90-91 0.5249081269259399 1102 92-93 0.39695955732532084 1102 94-95 0.2775775766754762 1102 96-97 0.24897188364428047 1102 98-99 0.08619716636195918 1102 100-101 0.46739211314715945 1103 1 0.18825158523971552 1103 2 0.2769169040158985 1103 3 0.42008915905719846 1103 4 0.31691150076851216 1103 5 0.130523676979017 1103 6 0.16824240163613524 1103 7 0.2701180066807609 1103 8 0.2169275016563077 1103 9 0.404699899443834 1103 10-11 0.36185888381232445 1103 12-13 0.3446085332295823 1103 14-15 0.49677680370355404 1103 16-17 0.5056355647858624 1103 18-19 0.5229805268651972 1103 20-21 0.43672867847230634 1103 22-23 0.40680594532317826 1103 24-25 0.48585787105119493 1103 26-27 0.5181104483292955 1103 28-29 0.4627634730483905 1103 30-31 0.5529696252362726 1103 32-33 0.47908336610903035 1103 34-35 0.35532776398543575 1103 36-37 0.3771191345328404 1103 38-39 0.5682590897819537 1103 40-41 0.6139789088631389 1103 42-43 0.582550091871191 1103 44-45 0.5098474026119177 1103 46-47 0.6089992360673833 1103 48-49 0.5349151059047585 1103 50-51 0.3966452035540087 1103 52-53 0.38592194308135674 1103 54-55 0.455664388264573 1103 56-57 0.5148257396989635 1103 58-59 0.516802127664711 1103 60-61 0.6623607430324228 1103 62-63 0.4695511418188758 1103 64-65 0.27412839456855664 1103 66-67 0.3859401287905939 1103 68-69 0.29643118731278406 1103 70-71 0.46350409886743194 1103 72-73 0.3969223165822662 1103 74-75 0.3176264028378668 1103 76-77 0.5054368195157224 1103 78-79 0.33370431755644603 1103 80-81 0.3613442136905647 1103 82-83 0.5094769760039348 1103 84-85 0.2925613948081107 1103 86-87 0.3427578580760837 1103 88-89 0.3088671274428414 1103 90-91 0.3064870344336832 1103 92-93 0.14858164476278546 1103 94-95 0.12166324626191738 1103 96-97 0.03274251609879997 1103 98-99 0.20036756208962103 1103 100-101 0.4758495802131293 1104 1 0.18931384806115403 1104 2 0.19183612534860117 1104 3 0.3058584983955157 1104 4 0.23134416115852474 1104 5 0.1682077423999715 1104 6 0.24690529820717444 1104 7 0.31324868941543826 1104 8 0.19982384984929524 1104 9 0.31297201913039885 1104 10-11 0.27053094385935594 1104 12-13 0.3039110531589486 1104 14-15 0.35340979136814354 1104 16-17 0.37950246675629984 1104 18-19 0.2890361094224403 1104 20-21 0.37258328536077556 1104 22-23 0.4093005650309607 1104 24-25 0.3020708828687333 1104 26-27 0.2535190587069991 1104 28-29 0.45462896468188063 1104 30-31 0.4360241455306877 1104 32-33 0.3725270502614748 1104 34-35 0.4712759794687926 1104 36-37 0.5517350856666283 1104 38-39 0.6365897294318401 1104 40-41 0.5035504009550706 1104 42-43 0.5320006812625735 1104 44-45 0.6391833397802813 1104 46-47 0.7830325875118902 1104 48-49 0.8116873656699397 1104 50-51 0.6710495245547676 1104 52-53 0.7042702252081696 1104 54-55 0.7019349278219238 1104 56-57 0.751459639996952 1104 58-59 0.8741337839457444 1104 60-61 0.9667575401136901 1104 62-63 0.8615948357343655 1104 64-65 0.8906220676844967 1104 66-67 0.8558534710663181 1104 68-69 0.8143721271978279 1104 70-71 0.8306411254653909 1104 72-73 0.9400336323873795 1104 74-75 0.8774901127138328 1104 76-77 0.9212237058228254 1104 78-79 0.7920949999939815 1104 80-81 0.8620582697304968 1104 82-83 0.9839477812861723 1104 84-85 0.9523830015098156 1104 86-87 0.9045613118628744 1104 88-89 0.731606476162181 1104 90-91 0.7460348882595262 1104 92-93 0.7372125407989856 1104 94-95 0.61920192175495 1104 96-97 0.5254906621795676 1104 98-99 0.5638203619639732 1104 100-101 0.6922874871839539 1105 1 0.30742716017759264 1105 2 0.35772089981330524 1105 3 0.37552469847317127 1105 4 0.3520863169051296 1105 5 0.3157498789702444 1105 6 0.4102233940814699 1105 7 0.45601649825056256 1105 8 0.248133724660498 1105 9 0.415254845827441 1105 10-11 0.5995171584983794 1105 12-13 0.43365120329278994 1105 14-15 0.5422470311771619 1105 16-17 0.6737647652928374 1105 18-19 0.6253022189920046 1105 20-21 0.5732545360844981 1105 22-23 0.5525953279424556 1105 24-25 0.5699177809122702 1105 26-27 0.5400419415000783 1105 28-29 0.7063910538065272 1105 30-31 0.9341400970272105 1105 32-33 0.6878016590563547 1105 34-35 0.8562955482857575 1105 36-37 0.8739202869101419 1105 38-39 0.833621427807067 1105 40-41 0.7419841475103013 1105 42-43 0.6450665082446179 1105 44-45 0.8130017957376694 1105 46-47 0.7308003884446848 1105 48-49 0.9371032260788681 1105 50-51 0.798264483359894 1105 52-53 0.7589363436283563 1105 54-55 1.0019348487985624 1105 56-57 0.9731370108161599 1105 58-59 0.8338562381161552 1105 60-61 0.7532253699690301 1105 62-63 0.8602644775425716 1105 64-65 0.9094456944034519 1105 66-67 0.9364139626548109 1105 68-69 0.8173216739462035 1105 70-71 0.6629135263477082 1105 72-73 0.7675571247139494 1105 74-75 0.6917034378427864 1105 76-77 0.7113932530664115 1105 78-79 0.73760312807293 1105 80-81 0.8345117816074428 1105 82-83 0.884365709472906 1105 84-85 0.9912336489481675 1105 86-87 0.7560869631075171 1105 88-89 0.7810408533237982 1105 90-91 0.8888821155670499 1105 92-93 1.0367144829296535 1105 94-95 0.9498262905558974 1105 96-97 0.8347951881632376 1105 98-99 0.8561568613241484 1105 100-101 0.9318714186801884 1106 1 0.258155950004042 1106 2 0.26650015209460065 1106 3 0.30831752347778263 1106 4 0.31761894370247035 1106 5 0.2856442236180712 1106 6 0.33126451805167534 1106 7 0.22198656654587978 1106 8 0.22181029017844622 1106 9 0.2075778059447373 1106 10-11 0.2905745449517667 1106 12-13 0.3642559320084686 1106 14-15 0.42547724689123356 1106 16-17 0.5574914387617795 1106 18-19 0.6332931573047773 1106 20-21 0.5081359118844802 1106 22-23 0.5251005832842637 1106 24-25 0.38049273739921574 1106 26-27 0.4856050312499889 1106 28-29 0.3373467057812647 1106 30-31 0.262367618410444 1106 32-33 0.1869447298012048 1106 34-35 0.11206893296967024 1106 36-37 0.2134276840037117 1106 38-39 0.45987952286183287 1106 40-41 0.5055013843711933 1106 42-43 0.4200713458508787 1106 44-45 0.35417299925792634 1106 46-47 0.36611407610241287 1106 48-49 0.3770374244760859 1106 50-51 0.2801149528235456 1106 52-53 0.2743807507714564 1106 54-55 0.41516634946211894 1106 56-57 0.30628276522624276 1106 58-59 0.5613691142749602 1106 60-61 0.5150863932865448 1106 62-63 0.5753839397531237 1106 64-65 0.3525366530824172 1106 66-67 0.37753521566848036 1106 68-69 0.22639715025361085 1106 70-71 0.25955950346545364 1106 72-73 0.24163987139758447 1106 74-75 0.177469067120132 1106 76-77 -0.07772672921855062 1106 78-79 0.2586318596449537 1106 80-81 0.3409504958656626 1106 82-83 0.42813804689759394 1106 84-85 0.5150160069472847 1106 86-87 0.47739005424903524 1106 88-89 0.4197889874806542 1106 90-91 0.3656812860889893 1106 92-93 0.4015959501489661 1106 94-95 0.3556193050016745 1106 96-97 0.40757716474058725 1106 98-99 0.4636465677614403 1106 100-101 0.7399529129189908 1107 1 0.15181896953097862 1107 2 0.19029994437405406 1107 3 0.31235288609650524 1107 4 0.30214341202007233 1107 5 0.3194288879869802 1107 6 0.2622392908322624 1107 7 0.2648489797533813 1107 8 0.2520701213152705 1107 9 0.3307627574331704 1107 10-11 0.3060444375024929 1107 12-13 0.46058370440609764 1107 14-15 0.48253335449689416 1107 16-17 0.37506615600319293 1107 18-19 0.33629011768749706 1107 20-21 0.2659883400695122 1107 22-23 0.38715150790135766 1107 24-25 0.3622373644345629 1107 26-27 0.2601390134092867 1107 28-29 0.36300274982764336 1107 30-31 0.327469263132123 1107 32-33 0.34886988925077844 1107 34-35 0.25912533130389903 1107 36-37 0.3677044265204614 1107 38-39 0.3936353900650502 1107 40-41 0.14601175335586447 1107 42-43 0.23746586150711124 1107 44-45 0.016160565097919743 1107 46-47 0.15530741962127337 1107 48-49 0.27652704546628115 1107 50-51 0.25028620197392115 1107 52-53 0.27669404789404695 1107 54-55 0.265608056379655 1107 56-57 0.20959045429505352 1107 58-59 0.19344208914998973 1107 60-61 0.1392179670102962 1107 62-63 0.05599322161346265 1107 64-65 0.2338011017908812 1107 66-67 0.16108579341631213 1107 68-69 0.3280515211069712 1107 70-71 0.19806940928944883 1107 72-73 0.32017680996563413 1107 74-75 0.25101775513229185 1107 76-77 0.3760411631196483 1107 78-79 0.3648504183530754 1107 80-81 0.2577834542593962 1107 82-83 0.3517837029115647 1107 84-85 0.4553405853407355 1107 86-87 0.6010481915410892 1107 88-89 0.6547414032345351 1107 90-91 0.5070947933381547 1107 92-93 0.319220141372174 1107 94-95 0.2187925421339294 1107 96-97 0.1850453750756884 1107 98-99 0.0029446738152110186 1107 100-101 0.3994209147315324 1108 1 0.4121245360593875 1108 2 0.28818739581917896 1108 3 0.3283883393850644 1108 4 0.35840740240785607 1108 5 0.36464252943802933 1108 6 0.31014813934105234 1108 7 0.43507702307420715 1108 8 0.46077996067270277 1108 9 0.48717940764055356 1108 10-11 0.510936752664783 1108 12-13 0.5205818938853213 1108 14-15 0.5741333610806052 1108 16-17 0.5640372041301234 1108 18-19 0.5560235596520755 1108 20-21 0.47694384240672605 1108 22-23 0.6276051256510442 1108 24-25 0.654915248100373 1108 26-27 0.7801493827555248 1108 28-29 0.819833145179544 1108 30-31 0.7617298711379092 1108 32-33 0.8351284480762473 1108 34-35 0.9178277639854358 1108 36-37 0.8279388066639868 1108 38-39 0.9404414668311247 1108 40-41 0.9541428432893682 1108 42-43 1.0238923049859423 1108 44-45 1.0060051894971593 1108 46-47 1.0687840721329565 1108 48-49 1.0466978927900072 1108 50-51 1.056737416668767 1108 52-53 1.223268254556757 1108 54-55 1.257918486625229 1108 56-57 1.2077048380596196 1108 58-59 1.1464127834024112 1108 60-61 0.9474529561471741 1108 62-63 0.9641720434582197 1108 64-65 1.0497431486669129 1108 66-67 1.055765948462728 1108 68-69 1.2377734004275354 1108 70-71 1.3536168037854708 1108 72-73 1.2322194477298005 1108 74-75 1.2382206651329533 1108 76-77 1.273367147384576 1108 78-79 1.2852924323105448 1108 80-81 1.4614979022151502 1108 82-83 1.459015910430164 1108 84-85 1.4749384439884423 1108 86-87 1.5072045793875581 1108 88-89 1.55502491432809 1108 90-91 1.6942944114828649 1108 92-93 1.7830591037791805 1108 94-95 1.7932820987209368 1108 96-97 1.700519155443061 1108 98-99 1.8599474801224112 1108 100-101 1.6505422031639512 1109 1 0.1165809670930642 1109 2 0.03725054410886486 1109 3 0.07742029178012899 1109 4 0.09375849270436731 1109 5 0.16542944587983044 1109 6 0.13929699835120601 1109 7 0.08666908605871981 1109 8 0.11167791500191271 1109 9 0.18880529867329443 1109 10-11 0.17409298755427471 1109 12-13 0.17707025001765686 1109 14-15 0.22333552607569374 1109 16-17 0.23138319389786943 1109 18-19 0.30416399187119225 1109 20-21 0.23802252820168235 1109 22-23 0.26161391509435106 1109 24-25 0.29404284515860013 1109 26-27 0.3189327038704661 1109 28-29 0.24816423305838242 1109 30-31 0.2753827056748861 1109 32-33 0.28780378850144217 1109 34-35 0.12352130721514953 1109 36-37 0.29507685629665303 1109 38-39 0.35955831105442826 1109 40-41 0.3877231278739188 1109 42-43 0.2379833930987516 1109 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74-75 -2.4891917584186025 2316 76-77 -2.4004149926291767 2316 78-79 -2.4731116950010055 2316 80-81 -2.5455952925157845 2316 82-83 -2.429271644305114 2316 84-85 -2.491653018212549 2316 86-87 -2.4327113499360777 2316 88-89 -2.5827639160558746 2316 90-91 -2.882192488238484 2316 92-93 -2.7401554404252124 2316 94-95 -2.6711543314531347 2316 96-97 -2.8518355925032353 2316 98-99 -2.7716068435123766 2316 100-101 -2.6422460843907842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7119.0 3 2283.0 4 2381.0 5 2701.0 6 3070.0 7 3758.0 8 4685.0 9 5678.0 10 6715.0 11 8312.0 12 10304.0 13 13543.0 14 12096.0 15 12371.0 16 14886.0 17 18569.0 18 17192.0 19 13781.0 20 14610.0 21 15877.0 22 17399.0 23 19070.0 24 21690.0 25 24659.0 26 28322.0 27 33001.0 28 38333.0 29 45274.0 30 54036.0 31 64552.0 32 79305.0 33 100401.0 34 133740.0 35 187261.0 36 276749.0 37 377657.0 38 282615.0 39 36590.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.618286786389696 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26.31508188798542 27.66179371159646 23.46075351590193 44-45 22.83344608584669 26.046955194808604 27.82373820690765 23.295860512437056 46-47 23.426408021741167 26.472495548683344 27.496804423203073 22.60429200637241 48-49 23.06758470239281 26.40749078760797 27.54094681119889 22.983977698800334 50-51 22.497415661590633 26.751001743553648 27.908055537782328 22.84352705707339 52-53 22.96341062300615 26.90542387352126 27.098073206746626 23.03309229672596 54-55 22.805345776856775 26.48287278651505 27.292706448855025 23.419074987773147 56-57 23.008535801369156 26.557553258498384 27.17686921007546 23.257041730056997 58-59 23.336654981360383 26.383544677248477 27.227504744527465 23.05229559686368 60-61 22.9050854077011 26.51271988681226 27.87362966373792 22.708565041748717 62-63 22.561740665838865 26.52342870508341 27.920482365467958 22.99434826360976 64-65 22.465194088525152 26.998029658353307 27.46105869558534 23.0757175575362 66-67 22.7138638643518 26.628540545999446 27.040773601286066 23.616821988362684 68-69 22.404653151227414 26.5055614307465 27.62557283111701 23.464212586909074 70-71 23.096288640669925 26.25379284758384 27.223851820629342 23.426066691116894 72-73 22.779818641944324 26.113140310022487 27.62763205652116 23.479408991512027 74-75 22.31886811318868 27.23892610738926 27.076167383261673 23.366038396160384 76-77 22.61435919043217 26.440531071777457 27.11507929665252 23.830030441137843 78-79 22.622666066013394 26.47844032032509 27.455051571480364 23.443842042181153 80-81 22.930083204711977 26.428833972129834 27.11907901361725 23.52200380954094 82-83 22.7500756557435 26.19492967826755 27.661514079465142 23.393480586523808 84-85 22.592495224424557 26.43689593093983 27.337482994353273 23.633125850282337 86-87 22.441864678489274 26.528323905983896 27.40416152816772 23.625649887359103 88-89 22.49162222553776 26.39074554882701 27.053894912256105 24.06373731337912 90-91 22.56462489814486 26.041846839866228 27.252285804268165 24.141242457720743 92-93 22.85059582492606 26.162478527705506 27.26946404753001 23.717461599838423 94-95 22.647001989116234 25.941080462338906 27.386608250595145 24.02530929794971 96-97 22.148527057684866 25.83393241552941 27.88729289170227 24.130247635083453 98-99 22.336111674934035 26.142616313891274 27.250661672584307 24.270610338590384 100-101 22.599183083470038 25.02908990580269 27.868854507485903 24.502872503241374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1688.0 1 1000.5 2 346.5 3 412.0 4 395.5 5 421.0 6 642.0 7 1199.0 8 1943.5 9 2405.0 10 2335.5 11 2158.0 12 2310.0 13 2505.0 14 2634.0 15 2833.5 16 3067.0 17 3194.5 18 3346.5 19 3582.0 20 3821.0 21 4219.5 22 4890.5 23 5920.0 24 6927.5 25 8255.0 26 10257.5 27 12508.5 28 14517.5 29 16775.0 30 19544.5 31 22717.0 32 26149.0 33 29169.5 34 31942.5 35 35557.0 36 40539.5 37 44637.0 38 47565.0 39 49561.0 40 51123.0 41 52959.0 42 54070.0 43 56543.0 44 60594.5 45 63640.0 46 67368.5 47 78156.0 48 95281.0 49 95884.0 50 82382.5 51 76167.5 52 75208.0 53 75142.5 54 73887.0 55 70026.0 56 64873.5 57 59189.5 58 54419.5 59 49410.5 60 41916.0 61 34665.0 62 27774.0 63 20957.5 64 15479.0 65 11422.0 66 8178.0 67 6167.5 68 4618.5 69 3246.5 70 2318.5 71 1513.5 72 1018.0 73 658.5 74 407.5 75 266.0 76 169.0 77 106.0 78 71.5 79 54.5 80 44.5 81 28.5 82 20.5 83 19.5 84 20.5 85 14.5 86 10.0 87 8.0 88 6.5 89 6.5 90 5.0 91 4.5 92 4.0 93 1.0 94 1.0 95 2.5 96 3.0 97 2.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32413419563540397 2 0.08748688815753808 3 0.0967876545506362 4 0.10230843032407949 5 0.11414576936984075 6 0.05674959601350252 7 0.05809248741785359 8 0.05943537882220466 9 0.06217089834958646 10-11 0.05983327257164202 12-13 0.058465512807951106 14-15 0.05672472765416269 16-17 0.05851524952663078 18-19 0.0662244409219795 20-21 0.0681641729504866 22-23 0.08452755339609776 24-25 0.09280871705626267 26-27 0.08810859714103395 28-29 0.08569636628506999 30-31 0.079529013168791 32-33 0.07144679638334477 34-35 0.3215230179047214 36-37 0.4983370528109453 38-39 0.4703850169129712 40-41 0.45073901303450187 42-43 0.48311761689496646 44-45 0.4920204895386273 46-47 0.4861515567344263 48-49 0.5051509832700599 50-51 0.5010974406976669 52-53 0.5004011266361515 54-55 0.4907770715716356 56-57 0.46889291535258115 58-59 0.45113690678393925 60-61 0.4103279291072706 62-63 0.4823218293960918 64-65 0.5059467707689346 66-67 0.5164412184103448 68-69 0.529795527375836 70-71 0.5274827699572314 72-73 0.5145263547411776 74-75 0.5166152969257237 76-77 0.48943418016728446 78-79 0.46914159894597945 80-81 0.47540842549961776 82-83 0.4848086653300753 84-85 0.4864748454058441 86-87 0.48540550595423126 88-89 0.4927168036001427 90-91 0.5071653203765865 92-93 0.5140289875543809 94-95 0.519873051999242 96-97 0.5125368859939908 98-99 0.5149242484906149 100-101 0.49204535789796716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2010587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.10330091686873 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33383191265037 36.74267047706593 2 13.653626605663 13.681835248601965 3 5.0278098978889805 7.557296168002278 4 2.461613264693293 4.933398005675348 5 1.3923046795065732 3.4879530162641155 6 0.8774930098609475 2.6379177795307145 7 0.6173531146181866 2.1652000211576814 8 0.44480412026471383 1.782892374934882 9 0.3406291416379852 1.5359979946088405 >10 1.7102230768955367 15.253115560852832 >50 0.10036420995424286 3.404373036841664 >100 0.03665257662336915 3.155723721208244 >500 0.0011979599064812418 0.4397093052330669 >1k 0.0016971098675150887 1.8454686071981807 >5k 3.9931996882707974E-4 1.3764486828243372 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9897 0.49224430477268577 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6435 0.32005578470367113 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5813 0.2891195456849169 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5374 0.26728512618454214 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4270 0.21237578876218735 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3683 0.18318033489722158 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3277 0.16298722711327587 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2954 0.14692226697974273 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2437 0.12120838342235378 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2409 0.11981575529932302 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2394 0.11906970451912799 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2173 0.10807788969092111 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2090 0.10394974204050857 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2022 0.10056764517029106 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.947343735933835E-5 2 0.0 0.0 0.0 0.0 9.947343735933835E-5 3 0.0 0.0 0.0 0.0 9.947343735933835E-5 4 0.0 0.0 0.0 0.0 9.947343735933835E-5 5 4.9736718679669175E-5 0.0 0.0 0.0 9.947343735933835E-5 6 4.9736718679669175E-5 0.0 0.0 0.0 9.947343735933835E-5 7 4.9736718679669175E-5 0.0 0.0 0.0 9.947343735933835E-5 8 4.9736718679669175E-5 0.0 0.0 0.0 9.947343735933835E-5 9 4.9736718679669175E-5 0.0 0.0 0.0 9.947343735933835E-5 10-11 4.9736718679669175E-5 0.0 0.0 7.460507801950376E-5 1.4921015603900752E-4 12-13 4.9736718679669175E-5 0.0 0.0 9.947343735933835E-5 2.4868359339834585E-4 14-15 4.9736718679669175E-5 0.0 0.0 9.947343735933835E-5 2.4868359339834585E-4 16-17 4.9736718679669175E-5 0.0 0.0 1.7407851537884212E-4 2.735519527381805E-4 18-19 7.460507801950376E-5 0.0 0.0 2.2381523405851127E-4 2.9842031207801505E-4 20-21 9.947343735933835E-5 0.0 0.0 2.4868359339834585E-4 3.978937494373534E-4 22-23 9.947343735933835E-5 2.4868359339834587E-5 0.0 4.2276210877718797E-4 3.978937494373534E-4 24-25 9.947343735933835E-5 4.9736718679669175E-5 0.0 5.471039054763608E-4 3.978937494373534E-4 26-27 9.947343735933835E-5 4.9736718679669175E-5 0.0 8.703925768942105E-4 4.724988274568571E-4 28-29 1.7407851537884212E-4 4.9736718679669175E-5 0.0 0.0037551222603150226 4.973671867966917E-4 30-31 1.989468747186767E-4 4.9736718679669175E-5 0.0 0.011265366780945068 4.973671867966917E-4 32-33 1.989468747186767E-4 4.9736718679669175E-5 0.0 0.027355195273818043 4.973671867966917E-4 34-35 2.4868359339834585E-4 4.9736718679669175E-5 0.0 0.05095526828732107 6.465773428356992E-4 36-37 2.4868359339834585E-4 4.9736718679669175E-5 0.0 0.08475136863015625 6.465773428356992E-4 38-39 2.4868359339834585E-4 4.9736718679669175E-5 0.0 0.1361045306669147 6.465773428356992E-4 40-41 2.4868359339834585E-4 4.9736718679669175E-5 0.0 0.1942218864441081 6.465773428356992E-4 42-43 2.4868359339834585E-4 4.9736718679669175E-5 0.0 0.2606452742408063 6.465773428356992E-4 44-45 2.4868359339834585E-4 4.9736718679669175E-5 0.0 0.341417705376589 6.714457021755338E-4 46-47 2.9842031207801505E-4 4.9736718679669175E-5 0.0 0.44201021890621994 7.21182420855203E-4 48-49 2.9842031207801505E-4 4.9736718679669175E-5 0.0 0.5470293003983413 7.460507801950375E-4 50-51 2.9842031207801505E-4 4.9736718679669175E-5 0.0 0.657743236179285 7.957874988747068E-4 52-53 2.9842031207801505E-4 4.9736718679669175E-5 0.0 0.7728091348446996 7.957874988747068E-4 54-55 2.9842031207801505E-4 4.9736718679669175E-5 0.0 0.9050342014546 7.957874988747068E-4 56-57 2.9842031207801505E-4 4.9736718679669175E-5 0.0 1.066529327007486 8.455242175543759E-4 58-59 2.9842031207801505E-4 4.9736718679669175E-5 0.0 1.2389167939512191 8.952609362340451E-4 60-61 3.232886714178496E-4 4.9736718679669175E-5 0.0 1.428388823761419 8.952609362340451E-4 62-63 3.481570307576842E-4 4.9736718679669175E-5 0.0 1.6245255738746942 8.952609362340451E-4 64-65 3.481570307576842E-4 7.460507801950376E-5 0.0 1.8328975567831682 9.449976549137142E-4 66-67 3.978937494373534E-4 9.947343735933835E-5 0.0 2.0758116908146724 9.449976549137142E-4 68-69 4.4763046811702254E-4 9.947343735933835E-5 0.0 2.356301915808667 9.449976549137142E-4 70-71 4.973671867966917E-4 9.947343735933835E-5 0.0 2.652931706014214 9.449976549137142E-4 72-73 5.471039054763608E-4 9.947343735933835E-5 0.0 2.9487408403615465 0.001143944529632391 74-75 5.719722648161955E-4 9.947343735933835E-5 0.0 3.2735216133397858 0.0013428914043510675 76-77 5.968406241560301E-4 9.947343735933835E-5 0.0 3.627398366745632 0.0013428914043510675 78-79 5.968406241560301E-4 9.947343735933835E-5 0.0 4.041829575143975 0.0013926281230307368 80-81 6.217089834958647E-4 9.947343735933835E-5 0.0 4.4855308424853035 0.0014174964823705713 82-83 6.963140615153684E-4 9.947343735933835E-5 0.0 4.934802622318756 0.001442364841710406 84-85 6.963140615153684E-4 9.947343735933835E-5 0.0 5.40476487712295 0.001442364841710406 86-87 6.963140615153684E-4 9.947343735933835E-5 0.0 5.907155472506288 0.001442364841710406 88-89 6.963140615153684E-4 9.947343735933835E-5 0.0 6.449434916270721 0.001442364841710406 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 465 0.0 23.428102 3 GTACATG 6990 0.0 23.01997 1 TACATGG 6880 0.0 22.71892 2 GAGTACT 5770 0.0 21.045942 12-13 ACATGGG 7275 0.0 20.89942 3 GTACTTT 6160 0.0 19.253311 14-15 AGTACTT 6060 0.0 19.101307 12-13 AGAGTAC 9835 0.0 18.989725 10-11 GTATAGG 490 0.0 18.405167 1 TATAGCG 160 0.001214308 17.762064 5 CATGGGA 3330 0.0 17.353128 4 ACTTTTT 7335 0.0 16.913506 16-17 TACTTTT 6870 0.0 16.677734 14-15 AGTACAT 5415 0.0 16.619476 2 CATGGGG 4075 0.0 16.505281 4 CTTTGCG 665 0.0 16.382057 2 GAGTACA 6315 0.0 16.160223 1 GTATTAT 590 0.0 15.285647 1 ACTATAC 530 7.2759576E-12 15.192711 3 GTATATG 690 0.0 15.134072 1 >>END_MODULE