FastQCFastQC Report
Fri 27 May 2016
SRR938951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010587
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106090.5276568484726103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63460.31562921674118055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55690.27698378632707765No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53550.2663401285296284No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40650.20217976143285515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.1763166677194272No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34960.17387956850412342No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30190.15015515369392124No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28280.14065544042610442No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26180.13021072950337387No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25410.12638100216503936No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24380.12125812014103345No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24030.11951733498724501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG2750.023.29819194-95
GTACATG72600.021.650211
TACATGG71600.021.2747542
GAGTACT62750.021.06307612-13
GTACTTT65650.019.98831214-15
ACATGGG73350.019.7967473
AGAGTAC104550.019.13279710-11
AGTACTT67000.018.7707412-13
GTCTTAG8300.018.3635521
CATGGGG39600.018.2146554
GTGTAGC10550.017.1559941
TTTGCGT3953.6561687E-1016.8191283
ACTTTTT79300.016.63664416-17
TACTTTT75700.016.42559614-15
AGTACAT55000.016.134352
GAGTACA66550.015.2446111
AACTCGA2851.8189894E-1214.9897536-37
CGCGATC2709.094947E-1214.93921322-23
GTATCAA206850.014.89819051
CATGGGA34300.014.3883754
Produced by FastQC (version 0.11.5)