##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938951_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2010587 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15734012007439 33.0 31.0 34.0 30.0 34.0 2 32.400224412074685 34.0 31.0 34.0 31.0 34.0 3 32.49045229079866 34.0 31.0 34.0 30.0 34.0 4 36.04706734898813 37.0 35.0 37.0 35.0 37.0 5 35.96954869398837 37.0 35.0 37.0 35.0 37.0 6 36.00695120380267 37.0 35.0 37.0 35.0 37.0 7 35.9420447859257 37.0 35.0 37.0 35.0 37.0 8 35.91244994620974 37.0 35.0 37.0 35.0 37.0 9 37.58129193116239 39.0 37.0 39.0 35.0 39.0 10-11 37.48897610498825 39.0 37.0 39.0 34.5 39.0 12-13 37.40257223387996 39.0 37.0 39.0 34.0 39.0 14-15 38.70426621678146 40.0 38.0 41.0 34.0 41.0 16-17 38.79869311797997 40.0 38.0 41.0 34.5 41.0 18-19 38.83204258258907 40.0 38.0 41.0 34.0 41.0 20-21 38.79730521484522 40.0 38.0 41.0 34.0 41.0 22-23 38.721020527835904 40.0 38.0 41.0 34.0 41.0 24-25 38.64234524544325 40.0 38.0 41.0 34.0 41.0 26-27 38.47908844531473 40.0 38.0 41.0 34.0 41.0 28-29 38.327555335829786 40.0 38.0 41.0 34.0 41.0 30-31 38.138125084863276 40.0 38.0 41.0 33.0 41.0 32-33 38.05106245091608 40.0 37.0 41.0 33.0 41.0 34-35 37.95692153584998 40.0 37.0 41.0 33.0 41.0 36-37 37.83231066350275 40.0 37.0 41.0 33.0 41.0 38-39 37.692600220731556 40.0 37.0 41.0 33.0 41.0 40-41 37.53246340496581 40.0 37.0 41.0 32.0 41.0 42-43 37.34791381820334 40.0 37.0 41.0 32.0 41.0 44-45 37.097790098115624 40.0 36.0 41.0 31.0 41.0 46-47 36.795697226730304 39.5 36.0 41.0 30.5 41.0 48-49 36.45894134399556 39.0 35.5 41.0 30.0 41.0 50-51 36.18510887616402 39.0 35.0 40.5 29.5 41.0 52-53 35.9217522047044 39.0 35.0 40.0 29.0 41.0 54-55 35.61144829843225 38.0 35.0 40.0 28.0 41.0 56-57 35.441671511852014 38.0 34.5 40.0 28.0 41.0 58-59 35.138067887636794 38.0 34.0 40.0 27.5 41.0 60-61 34.74009182393003 37.5 34.0 40.0 26.5 41.0 62-63 34.30552843522812 37.0 33.5 40.0 26.0 41.0 64-65 33.87627021362418 36.0 33.0 39.0 26.0 41.0 66-67 33.392844229073404 36.0 32.5 39.0 25.0 40.0 68-69 32.88165968446031 35.0 32.0 38.5 23.5 40.0 70-71 32.33387637540679 35.0 31.5 37.5 22.0 40.0 72-73 31.805542858876535 35.0 31.0 37.0 21.5 39.0 74-75 31.279472363046217 34.5 31.0 36.5 19.5 39.0 76-77 29.41010660070915 32.5 28.5 34.5 17.5 36.5 78-79 30.37224775650096 34.0 30.0 35.0 18.0 37.0 80-81 30.354752368338204 34.0 30.0 35.0 17.5 37.0 82-83 29.94595533543189 34.0 30.0 35.0 12.5 36.5 84-85 29.488974861570277 34.0 29.0 35.0 7.0 36.0 86-87 28.79998080162659 33.5 29.0 35.0 2.0 35.5 88-89 28.08920379968636 33.0 27.0 35.0 2.0 35.0 90-91 27.48585686667625 33.0 26.0 35.0 2.0 35.0 92-93 27.05999864716125 32.0 25.5 34.0 2.0 35.0 94-95 26.46811577912321 32.0 25.0 34.0 2.0 35.0 96-97 25.570384917439533 32.0 23.5 34.0 2.0 35.0 98-99 24.29887739252268 31.5 10.0 34.0 2.0 35.0 100-101 18.703295604716434 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1571603556833523 1101 2 0.1708220262296294 1101 3 0.16109345551208776 1101 4 0.15939827502153037 1101 5 0.15007400690672767 1101 6 0.2053751346604784 1101 7 0.2010235546632444 1101 8 0.16657488006831755 1101 9 0.30415497085479615 1101 10-11 0.18626402930387798 1101 12-13 0.15574614406350662 1101 14-15 0.3309471095826808 1101 16-17 0.2874325983512094 1101 18-19 0.3133113543543047 1101 20-21 0.3537883361678169 1101 22-23 0.35184800919445536 1101 24-25 0.31400531790193753 1101 26-27 0.2483217524053245 1101 28-29 0.3484852906372211 1101 30-31 0.24580542184140342 1101 32-33 0.33234843100820655 1101 34-35 0.3457995415628474 1101 36-37 0.4308703865121686 1101 38-39 0.339727206840557 1101 40-41 0.4321674605486834 1101 42-43 0.33042417572308125 1101 44-45 0.48325081520820135 1101 46-47 0.35026722493750384 1101 48-49 0.4116079881970549 1101 50-51 0.2920603137219189 1101 52-53 0.2356520659211938 1101 54-55 0.060455597819199625 1101 56-57 0.15263741375455453 1101 58-59 0.2853926858525071 1101 60-61 0.18938552728604208 1101 62-63 0.2537862718605268 1101 64-65 0.19901310731081168 1101 66-67 0.05570744646100678 1101 68-69 0.195933858114536 1101 70-71 0.3183765887357026 1101 72-73 0.39478936972535195 1101 74-75 0.327822421085763 1101 76-77 0.0718094983902553 1101 78-79 0.43841782005242536 1101 80-81 0.4449665171696715 1101 82-83 0.44749313471981367 1101 84-85 0.597557683056678 1101 86-87 0.700460691923336 1101 88-89 0.5851869464972914 1101 90-91 0.7031530240803789 1101 92-93 0.6904925683103436 1101 94-95 0.49788045630293354 1101 96-97 0.5374145438559417 1101 98-99 0.9387795411994198 1101 100-101 1.371627622403846 1102 1 0.13866823120097393 1102 2 0.14136388616760343 1102 3 0.14713092849096654 1102 4 0.12841036611105494 1102 5 0.0687436000556616 1102 6 0.04350491346043839 1102 7 0.09665649859751824 1102 8 0.06953047972996984 1102 9 0.1363569644747713 1102 10-11 0.17748983468543855 1102 12-13 0.15283615550774954 1102 14-15 0.2093279982528884 1102 16-17 0.11690483639142712 1102 18-19 0.17130186724427432 1102 20-21 0.1749261812227516 1102 22-23 0.17329877407888006 1102 24-25 0.16662146036339465 1102 26-27 0.14944074486981407 1102 28-29 0.2766392295017539 1102 30-31 0.2428133332950395 1102 32-33 0.27211882746546934 1102 34-35 0.24294069273187802 1102 36-37 0.2874292635390958 1102 38-39 0.30019841102903655 1102 40-41 0.3837353768675129 1102 42-43 0.378984799793578 1102 44-45 0.29632019384672503 1102 46-47 0.5144967842512855 1102 48-49 0.5062065275359444 1102 50-51 0.5102773894142345 1102 52-53 0.4929349155925138 1102 54-55 0.530468368928851 1102 56-57 0.5363849327868238 1102 58-59 0.47532147724112406 1102 60-61 0.49474275899598297 1102 62-63 0.5309881825512193 1102 64-65 0.6349004782866743 1102 66-67 0.3960641529521567 1102 68-69 0.3292426812374245 1102 70-71 0.2931597286595178 1102 72-73 0.4894791312760134 1102 74-75 0.48118713854569606 1102 76-77 0.3381924547639237 1102 78-79 0.5124620600608303 1102 80-81 0.48635845754904494 1102 82-83 0.6191255147349608 1102 84-85 0.7039128304773037 1102 86-87 0.845225366260042 1102 88-89 0.5706956070787896 1102 90-91 0.6753927228812238 1102 92-93 0.7692991606355157 1102 94-95 0.9461057696374091 1102 96-97 0.9204474888959417 1102 98-99 1.5551967750102413 1102 100-101 1.4180868184320872 1103 1 0.12382952571167749 1103 2 0.026269520006991343 1103 3 0.08644857524430449 1103 4 0.08783789393836372 1103 5 0.0859625572457503 1103 6 0.08756174954331897 1103 7 0.12258447218105317 1103 8 0.06404183569444655 1103 9 0.16724397609039698 1103 10-11 0.1283416943111746 1103 12-13 0.12214226885966184 1103 14-15 0.18380818373941565 1103 16-17 0.14220150230794815 1103 18-19 0.17901225985597335 1103 20-21 0.15825576385643103 1103 22-23 0.16589240452532295 1103 24-25 0.16656330648661566 1103 26-27 0.1557175767980823 1103 28-29 0.15859064640067544 1103 30-31 0.07156152646761882 1103 32-33 0.06474291431436541 1103 34-35 0.0793489686506561 1103 36-37 0.11665594140016111 1103 38-39 0.11321070611100481 1103 40-41 0.13406536630785126 1103 42-43 0.10306439973818726 1103 44-45 0.24307910247968323 1103 46-47 0.16010543624983598 1103 48-49 0.04009400871031943 1103 50-51 0.049497175364692225 1103 52-53 -0.03936509998731452 1103 54-55 -4.6191999042832776E-4 1103 56-57 0.03646337289969637 1103 58-59 0.058463398666859234 1103 60-61 0.02607363345718028 1103 62-63 0.04041992991562893 1103 64-65 -0.08101918605456149 1103 66-67 0.056709774587353934 1103 68-69 0.18087077340077684 1103 70-71 0.08339166254911135 1103 72-73 -0.1286603341627277 1103 74-75 0.05365309012704955 1103 76-77 -0.1100946836542036 1103 78-79 0.19136123692651452 1103 80-81 0.2672754927940808 1103 82-83 0.3082765438692441 1103 84-85 0.3868654677961878 1103 86-87 0.696370646004528 1103 88-89 0.5006504796704085 1103 90-91 0.438305317874903 1103 92-93 0.5262089231296727 1103 94-95 0.3841372690228688 1103 96-97 0.2476314780246831 1103 98-99 0.4104509642532328 1103 100-101 0.9834444728887846 1104 1 -0.05492192813454011 1104 2 -0.024974579712001344 1104 3 -0.011798001319270668 1104 4 -0.04015787823438188 1104 5 -0.017493458069509416 1104 6 0.004709228977233693 1104 7 -0.024795474079695623 1104 8 0.04406206219731956 1104 9 -0.046246443381377844 1104 10-11 0.026097173295134724 1104 12-13 0.01939327912360511 1104 14-15 0.005282898488417231 1104 16-17 0.0360107704184216 1104 18-19 -0.030202843589961503 1104 20-21 3.9091954505465765E-5 1104 22-23 0.027279044845897715 1104 24-25 0.025474296078350278 1104 26-27 -0.024273625840493196 1104 28-29 0.026456718511447264 1104 30-31 -0.06429920886021279 1104 32-33 -0.05446305749163116 1104 34-35 0.03207309973877415 1104 36-37 0.023737658169693532 1104 38-39 0.12723103661164004 1104 40-41 0.16303351752483763 1104 42-43 0.03140649505956361 1104 44-45 0.1350335228089108 1104 46-47 0.2030130013439404 1104 48-49 0.1220701093886376 1104 50-51 0.15677205735907762 1104 52-53 0.09833111619219181 1104 54-55 0.31928237286725647 1104 56-57 0.12369600315158635 1104 58-59 0.10725545939305903 1104 60-61 0.21106216717367943 1104 62-63 0.19941260550205442 1104 64-65 0.23678549554250594 1104 66-67 0.03627620474755133 1104 68-69 0.04525981840421167 1104 70-71 0.11351108487339445 1104 72-73 0.03337719417135787 1104 74-75 0.19856852827597393 1104 76-77 0.12746020699958294 1104 78-79 0.2626657614657404 1104 80-81 0.47555872856761283 1104 82-83 0.33556905772853796 1104 84-85 0.5269281635878862 1104 86-87 0.7119874216919762 1104 88-89 0.7167210782305702 1104 90-91 0.745622541813912 1104 92-93 0.739124967224722 1104 94-95 1.0617005750054993 1104 96-97 0.9470063241504505 1104 98-99 1.2003545328620397 1104 100-101 1.2591901527369878 1105 1 0.02622662892532901 1105 2 0.07254024111833957 1105 3 0.11687046040681537 1105 4 0.1087466209867074 1105 5 0.11453955472752853 1105 6 0.12502808294892276 1105 7 0.19840362315748905 1105 8 0.12680241305048412 1105 9 0.11459901169568809 1105 10-11 0.23759370179912764 1105 12-13 0.20790296712358725 1105 14-15 0.21482965739816962 1105 16-17 0.04439014363676819 1105 18-19 0.11424263108489896 1105 20-21 0.2441378911682719 1105 22-23 0.20451987062942578 1105 24-25 0.07888710407846133 1105 26-27 0.09037780477199675 1105 28-29 0.246637650386468 1105 30-31 0.16154019039370127 1105 32-33 0.2458845662497211 1105 34-35 0.20966083805345903 1105 36-37 0.23065228080946554 1105 38-39 0.2485707406322959 1105 40-41 0.2232693337544447 1105 42-43 0.3019865475626844 1105 44-45 0.2624538106330476 1105 46-47 0.243742429062074 1105 48-49 0.06796927137229147 1105 50-51 0.217639665316824 1105 52-53 0.2291525692420464 1105 54-55 0.3584135532180497 1105 56-57 0.3063188462749409 1105 58-59 0.21708751895770462 1105 60-61 0.14655079228845835 1105 62-63 0.19512835427732256 1105 64-65 0.10288015603973122 1105 66-67 -0.009767938752538896 1105 68-69 -0.006611507339009393 1105 70-71 0.17870851419095857 1105 72-73 0.22505466508680882 1105 74-75 0.14094311689355266 1105 76-77 0.030811218735603774 1105 78-79 0.08931341160089445 1105 80-81 0.16158036621602534 1105 82-83 0.3322840568355723 1105 84-85 0.4029858253500116 1105 86-87 0.35908478847571956 1105 88-89 0.6458751911591598 1105 90-91 0.38322577924274626 1105 92-93 0.6663781733339675 1105 94-95 0.8325520727351581 1105 96-97 0.6313232963126261 1105 98-99 0.8760990514069782 1105 100-101 1.0216896720115791 1106 1 0.014413219842609237 1106 2 0.06422616086537403 1106 3 -0.05126340187199219 1106 4 -0.013958182438869926 1106 5 0.016577393188555334 1106 6 -0.019720206620789327 1106 7 0.049482802963566996 1106 8 0.04128140666585978 1106 9 0.004363297108866959 1106 10-11 0.1646715489145194 1106 12-13 0.18957262946629072 1106 14-15 0.10588574874436318 1106 16-17 -0.05771839244008703 1106 18-19 0.04210363713431775 1106 20-21 0.07700468964807072 1106 22-23 0.03446531360432914 1106 24-25 0.12141880500113444 1106 26-27 0.0820117932602713 1106 28-29 0.11134440510688393 1106 30-31 0.009504637415950867 1106 32-33 0.05683865901698226 1106 34-35 0.08811617133024185 1106 36-37 0.0533743269366056 1106 38-39 0.03384793674046449 1106 40-41 0.10407909571374319 1106 42-43 0.17726124995466108 1106 44-45 0.16924786675130576 1106 46-47 0.2544976593905304 1106 48-49 0.22994997783040816 1106 50-51 0.17081430315661095 1106 52-53 0.178618375618953 1106 54-55 0.17871603438948114 1106 56-57 0.2559494613899105 1106 58-59 0.13137567471999034 1106 60-61 0.2317209769910633 1106 62-63 0.2818254957472064 1106 64-65 0.2054386783783002 1106 66-67 0.2077883565095462 1106 68-69 0.13520361839557893 1106 70-71 0.2974469883718527 1106 72-73 0.3087046225006418 1106 74-75 0.1196513008028397 1106 76-77 -0.0460988485134699 1106 78-79 0.19979760900773513 1106 80-81 0.13869594919963646 1106 82-83 0.1847881084895242 1106 84-85 0.14131678262721792 1106 86-87 0.4812539000169558 1106 88-89 0.5848135537068941 1106 90-91 0.7301673455301447 1106 92-93 0.7198188668206953 1106 94-95 0.749633977853069 1106 96-97 0.5289681819851388 1106 98-99 0.7445095587385531 1106 100-101 1.2090772071889724 1107 1 0.045462780040466555 1107 2 0.08237714723722434 1107 3 0.050604378786339055 1107 4 0.006808859768902664 1107 5 -0.018121813022204947 1107 6 0.037947308171276006 1107 7 0.11220072407386539 1107 8 0.012890509076498802 1107 9 -0.026531124311212295 1107 10-11 0.004151285133481508 1107 12-13 0.05656644095788721 1107 14-15 0.0729909393520245 1107 16-17 0.08572971351342318 1107 18-19 0.05101872835290777 1107 20-21 0.004884080401296842 1107 22-23 0.015600663791900615 1107 24-25 -0.029716690550721125 1107 26-27 0.02188511580657604 1107 28-29 -0.048092644138527874 1107 30-31 0.05280514849671647 1107 32-33 0.10246449736789032 1107 34-35 0.11383424582492552 1107 36-37 0.07637596905175315 1107 38-39 0.04342130259529853 1107 40-41 0.06529026590624198 1107 42-43 0.10590568290363223 1107 44-45 0.025194243371117864 1107 46-47 0.18256000863774346 1107 48-49 0.11335529154657564 1107 50-51 0.014705344105223617 1107 52-53 -0.059939199664711396 1107 54-55 0.01054522333697605 1107 56-57 0.0673883021247903 1107 58-59 0.34461517791500285 1107 60-61 0.34242201880183387 1107 62-63 0.2963102946532672 1107 64-65 0.20852629093536734 1107 66-67 0.19469717500870587 1107 68-69 0.14190754343172074 1107 70-71 0.3612788259568376 1107 72-73 0.3939711754824131 1107 74-75 0.13700391144510604 1107 76-77 0.30491679669341565 1107 78-79 0.44602127050344365 1107 80-81 0.3927106350022598 1107 82-83 0.2310372588603542 1107 84-85 0.45957433605611087 1107 86-87 0.5097254998461267 1107 88-89 0.7259255553830961 1107 90-91 0.68355578696438 1107 92-93 0.9440915190872765 1107 94-95 1.0716136088082422 1107 96-97 1.1192079478383654 1107 98-99 1.039666597277133 1107 100-101 1.1707150716773818 1108 1 0.07823526341659459 1108 2 0.053421159351252356 1108 3 0.11257562442462898 1108 4 0.07451822180721734 1108 5 0.017315016262145377 1108 6 0.16184453642856766 1108 7 0.15127299677121187 1108 8 0.14754593405510263 1108 9 0.14009233674613597 1108 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2316 70-71 -0.6662933834104336 2316 72-73 -0.7582612642101338 2316 74-75 -0.9495598934433218 2316 76-77 -0.8123629181926191 2316 78-79 -0.976265558933509 2316 80-81 -1.2188910698303168 2316 82-83 -1.2054032049817671 2316 84-85 -1.2185022085328434 2316 86-87 -1.1993578534345453 2316 88-89 -1.3943924716920968 2316 90-91 -1.533017127208872 2316 92-93 -1.5123823897932773 2316 94-95 -1.5571072348312178 2316 96-97 -1.5314971905961663 2316 98-99 -1.6043621667857408 2316 100-101 -1.0143483608607191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 12.0 5 28.0 6 72.0 7 206.0 8 545.0 9 1062.0 10 1752.0 11 2646.0 12 4114.0 13 5390.0 14 6583.0 15 8864.0 16 11729.0 17 16193.0 18 16528.0 19 12521.0 20 12324.0 21 13239.0 22 14648.0 23 16072.0 24 18573.0 25 21765.0 26 26309.0 27 31489.0 28 38651.0 29 46783.0 30 56129.0 31 68236.0 32 83764.0 33 107347.0 34 143645.0 35 206321.0 36 317822.0 37 437435.0 38 250183.0 39 11598.0 >>END_MODULE >>Per base sequence content warn #Base G 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22.79772657639555 18-19 22.210959576579786 27.294568555980998 28.452891318149813 22.041580549289396 20-21 23.31344030375209 25.95125204728768 28.315561574803777 22.419746074156453 22-23 22.846934750896132 25.91457121726143 28.41921289653221 22.819281135310234 24-25 23.156237977681606 26.053083594886754 28.317202443967137 22.473475983464503 26-27 22.73288845496365 25.892687061042373 28.984520441045326 22.389904042948654 28-29 22.40795847182937 26.829105131983845 28.482975369879544 22.279961026307245 30-31 22.800803944320737 26.042717872939598 28.17227008828765 22.98420809445202 32-33 22.6465206429764 26.17225715674079 28.43691916838217 22.744303031900635 34-35 23.28139036762826 26.13057652762261 28.17674171510775 22.411291389641384 36-37 23.083009517233354 25.73468653722645 28.664179613092205 22.51812433244799 38-39 22.63348130668536 26.381769258567207 28.50153788989121 22.48321154485622 40-41 22.839685786947715 26.39467729893762 28.276339566163948 22.489297347950714 42-43 22.59315934122642 26.433136938766527 27.716279609956462 23.257424110050593 44-45 22.961721180343396 26.056769029178824 27.88268685616203 23.098822934315752 46-47 23.404281246090953 26.53935384652879 27.558224464384168 22.498140442996093 48-49 23.127658045280246 26.505136597508063 27.541856635846333 22.82534872136536 50-51 22.60622285443574 26.79366200423014 27.909637677825593 22.69047746350853 52-53 23.068561569332736 26.992987620033354 26.985377902075363 22.953072908558543 54-55 22.829875056389003 26.57793470265151 27.218294955652254 23.373895285307224 56-57 23.053938974040914 26.625308927193895 27.176914005710774 23.143838093054416 58-59 23.449045244925813 26.394431822251224 27.182542445716834 22.97398048710613 60-61 22.9241376291803 26.64197871043022 27.79998826198844 22.633895398401044 62-63 22.597955393477832 26.66302091260237 27.796686293058944 22.942337400860854 64-65 22.492811130754482 27.120545456308857 27.362292400650862 23.0243510122858 66-67 22.793459530665693 26.652569212522977 26.98705837793396 23.566912878877368 68-69 22.468765714132235 26.59421814436087 27.496168922538374 23.44084721896852 70-71 23.104880865578494 26.377135820104186 27.123261576475716 23.394721737841603 72-73 22.793442909956145 26.26839823394859 27.44283137213162 23.49532748396364 74-75 22.32539551882112 27.372876677308668 26.867551615523226 23.434176188346985 76-77 22.614817945535744 26.55407521131122 27.015925689739028 23.81518115341401 78-79 22.671708454984508 26.651305874570387 27.259702665466317 23.41728300497879 80-81 22.93462582936267 26.538635617582983 27.00034318455371 23.526395368500634 82-83 22.72510465849028 26.378241777152645 27.47889049317438 23.417763071182694 84-85 22.50467775002462 26.670337245633352 27.17312698512172 23.651858019220306 86-87 22.40676604254315 26.708673161294882 27.158980991538463 23.725579804623504 88-89 22.477130329335747 26.59987786190903 26.78593238944817 24.13705941930706 90-91 22.545052832614545 26.240302578281522 26.98831004287774 24.226334546226198 92-93 22.76273048617145 26.493979121520233 26.916144389673264 23.827146002635054 94-95 22.562599350232073 26.28277438569476 27.07051354744241 24.084112716630756 96-97 22.027049811821126 26.158430348947842 27.570207108670253 24.244312730560775 98-99 22.1877242815158 26.47793902974604 26.878593167070115 24.45574352166805 100-101 22.337932337932337 25.434501487133065 27.664405295984245 24.563160878950352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 564.0 1 408.0 2 278.5 3 329.0 4 318.0 5 316.5 6 499.5 7 1061.5 8 1727.5 9 2099.0 10 1970.0 11 1728.0 12 1813.5 13 1939.0 14 2107.0 15 2273.0 16 2422.5 17 2549.0 18 2718.5 19 2987.0 20 3294.0 21 3846.5 22 4642.5 23 5703.0 24 6850.0 25 8256.0 26 10161.5 27 12390.5 28 14625.0 29 17076.0 30 19721.5 31 23029.5 32 26604.5 33 29507.5 34 32241.5 35 35661.5 36 40604.5 37 44874.0 38 47763.0 39 50120.0 40 51777.5 41 53387.0 42 54631.5 43 57142.0 44 61435.0 45 64803.5 46 68252.0 47 79480.0 48 97042.5 49 96834.5 50 83064.5 51 77161.0 52 76201.5 53 76055.0 54 74663.0 55 70467.5 56 65293.0 57 59482.0 58 54167.5 59 49076.0 60 41650.5 61 34051.5 62 26991.0 63 20078.0 64 14688.0 65 10605.5 66 7339.5 67 5558.0 68 4131.5 69 2954.5 70 2103.0 71 1253.5 72 761.5 73 473.5 74 301.5 75 200.0 76 103.5 77 47.0 78 30.0 79 14.0 80 9.5 81 5.5 82 3.5 83 4.0 84 4.0 85 3.0 86 1.0 87 1.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38879192991897393 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.714457021755338E-4 16-17 0.002536572652663128 18-19 3.7302539009751877E-4 20-21 0.0 22-23 0.0 24-25 1.740785153788421E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01198654920180027 36-37 0.02208310309377311 38-39 0.010071685532633008 40-41 0.005172618742685594 42-43 0.004650383196549067 44-45 0.0018153902318079247 46-47 0.0013677597636909023 48-49 0.0031582816361589924 50-51 7.21182420855203E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.449976549137142E-4 60-61 0.0012434179669917294 62-63 0.001442364841710406 64-65 6.714457021755338E-4 66-67 0.002909598042760647 68-69 0.0027852562460614736 70-71 4.9736718679669175E-5 72-73 0.0 74-75 0.0 76-77 0.0905208279969979 78-79 0.002834992964741143 80-81 3.481570307576842E-4 82-83 0.0 84-85 2.4868359339834585E-4 86-87 0.09201292955738798 88-89 0.43542010368116374 90-91 0.3879215373420797 92-93 0.0 94-95 0.001143944529632391 96-97 0.0 98-99 0.0 100-101 3.481570307576842E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2010587.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.922909773988195 #Duplication Level Percentage of deduplicated Percentage of total 1 71.39964735963899 34.21678858310553 2 14.546499048594042 13.942211228663549 3 5.37693482389132 7.730350872778765 4 2.6210174162123434 5.024271246127832 5 1.5187808250053847 3.6392198221598204 6 0.9727510774370941 2.79702372699406 7 0.6904323847196752 2.316127021457235 8 0.47260952081943347 1.8119058739646006 9 0.36157597915782297 1.5594995723059608 >10 1.8826703608106774 16.11027618696643 >50 0.11279062975476191 3.6603151341943447 >100 0.04075673405544876 3.4207433600244053 >500 0.0013511740805784201 0.4626295182428637 >1k 0.0017669199515256244 1.9200021575912634 >5k 3.118094032104043E-4 0.8602079866552811 >10k+ 1.0393646773680144E-4 0.5284277087681457 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10609 0.5276568484726103 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6346 0.31562921674118055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5569 0.27698378632707765 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5355 0.2663401285296284 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4065 0.20217976143285515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3545 0.1763166677194272 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3496 0.17387956850412342 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3019 0.15015515369392124 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2828 0.14065544042610442 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2618 0.13021072950337387 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2541 0.12638100216503936 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2438 0.12125812014103345 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2403 0.11951733498724501 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.9736718679669175E-5 2 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 3 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 4 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 5 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 6 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 7 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 8 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 9 9.947343735933835E-5 0.0 0.0 0.0 4.9736718679669175E-5 10-11 1.4921015603900752E-4 0.0 0.0 7.460507801950376E-5 9.947343735933835E-5 12-13 1.989468747186767E-4 0.0 0.0 9.947343735933835E-5 9.947343735933835E-5 14-15 2.4868359339834585E-4 0.0 0.0 9.947343735933835E-5 1.4921015603900752E-4 16-17 2.4868359339834585E-4 0.0 0.0 1.7407851537884212E-4 2.4868359339834585E-4 18-19 2.98420312078015E-4 0.0 0.0 2.2381523405851127E-4 2.4868359339834585E-4 20-21 3.481570307576842E-4 0.0 0.0 2.4868359339834585E-4 3.232886714178496E-4 22-23 3.481570307576842E-4 0.0 0.0 4.2276210877718797E-4 3.481570307576842E-4 24-25 3.481570307576842E-4 0.0 0.0 5.471039054763608E-4 3.481570307576842E-4 26-27 3.481570307576842E-4 0.0 0.0 8.455242175543758E-4 3.978937494373534E-4 28-29 3.7302539009751877E-4 0.0 0.0 0.003829727338334526 3.978937494373534E-4 30-31 4.4763046811702254E-4 0.0 0.0 0.011563787093023081 4.4763046811702254E-4 32-33 4.724988274568571E-4 0.0 0.0 0.02787743081995457 4.973671867966917E-4 34-35 5.222355461365262E-4 0.0 0.0 0.051676450708176266 4.973671867966917E-4 36-37 5.471039054763608E-4 0.0 0.0 0.08611912839384717 4.973671867966917E-4 38-39 5.471039054763608E-4 0.0 0.0 0.1379696576174023 4.973671867966917E-4 40-41 5.471039054763608E-4 0.0 0.0 0.19680819581545092 4.973671867966917E-4 42-43 5.471039054763608E-4 0.0 0.0 0.2637538191582856 4.973671867966917E-4 44-45 5.471039054763608E-4 0.0 0.0 0.34509822255888456 4.973671867966917E-4 46-47 5.471039054763608E-4 0.0 0.0 0.4472325743675852 5.222355461365262E-4 48-49 5.471039054763608E-4 0.0 0.0 0.5536442839827374 5.968406241560301E-4 50-51 5.471039054763608E-4 0.0 0.0 0.6655021642933133 5.968406241560301E-4 52-53 5.471039054763608E-4 0.0 0.0 0.7816871391290205 6.465773428356992E-4 54-55 5.471039054763608E-4 0.0 0.0 0.9155037807366704 6.963140615153684E-4 56-57 5.719722648161955E-4 0.0 0.0 1.0781428508191886 7.460507801950375E-4 58-59 5.968406241560301E-4 0.0 0.0 1.2524203130727494 7.460507801950375E-4 60-61 5.968406241560301E-4 0.0 0.0 1.4445781256916512 7.460507801950375E-4 62-63 5.968406241560301E-4 0.0 0.0 1.6419085570532386 7.957874988747068E-4 64-65 5.968406241560301E-4 0.0 0.0 1.8519218516781417 7.957874988747068E-4 66-67 6.465773428356992E-4 0.0 0.0 2.0965270341447546 7.957874988747068E-4 68-69 6.465773428356992E-4 0.0 0.0 2.379006727885936 7.957874988747068E-4 70-71 6.465773428356992E-4 0.0 0.0 2.67906835168038 7.957874988747068E-4 72-73 6.465773428356992E-4 0.0 0.0 2.9800003680517184 9.201292955738797E-4 74-75 6.465773428356992E-4 0.0 0.0 3.3089341570397104 0.0010444710922730526 76-77 6.465773428356992E-4 0.0 0.0 3.66671524286191 0.0010444710922730526 78-79 6.465773428356992E-4 0.0 0.0 4.083359735241499 0.0010942078109527217 80-81 6.714457021755338E-4 0.0 0.0 4.530642046327764 0.0011190761702925562 82-83 6.963140615153684E-4 0.0 0.0 4.985359996856639 0.0011936812483120602 84-85 6.963140615153684E-4 0.0 0.0 5.460768422356257 0.0011936812483120602 86-87 6.963140615153684E-4 0.0 0.0 5.966814666562551 0.0012185496076518947 88-89 6.963140615153684E-4 0.0 0.0 6.513172521258717 0.0012434179669917292 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 275 0.0 23.298191 94-95 GTACATG 7260 0.0 21.65021 1 TACATGG 7160 0.0 21.274754 2 GAGTACT 6275 0.0 21.063076 12-13 GTACTTT 6565 0.0 19.988312 14-15 ACATGGG 7335 0.0 19.796747 3 AGAGTAC 10455 0.0 19.132797 10-11 AGTACTT 6700 0.0 18.77074 12-13 GTCTTAG 830 0.0 18.363552 1 CATGGGG 3960 0.0 18.214655 4 GTGTAGC 1055 0.0 17.155994 1 TTTGCGT 395 3.6561687E-10 16.819128 3 ACTTTTT 7930 0.0 16.636644 16-17 TACTTTT 7570 0.0 16.425596 14-15 AGTACAT 5500 0.0 16.13435 2 GAGTACA 6655 0.0 15.244611 1 AACTCGA 285 1.8189894E-12 14.98975 36-37 CGCGATC 270 9.094947E-12 14.939213 22-23 GTATCAA 20685 0.0 14.8981905 1 CATGGGA 3430 0.0 14.388375 4 >>END_MODULE