FastQCFastQC Report
Fri 27 May 2016
SRR938950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022595
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99490.4918928406329493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65970.3261651492266124No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59660.29496760349946477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51570.2549694822740094No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44270.21887723444387036No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36160.17878023034764745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.16834808748167576No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30440.1504997293081413No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25280.1249879486501252No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24680.12202146252710008No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23760.1174728504717949No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21980.10867227497348704No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21350.10555746454431066No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21010.10387645574126307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT4250.028.953143
GTACATG72650.022.9716871
TACATGG72900.022.3976692
GAGTACT58850.021.23117812-13
ACATGGG75350.020.4760253
CGTACAC2601.5555997E-820.0230663
GTACTTT63000.019.72017714-15
AGTACTT62300.019.2198112-13
GTCCTAC5000.018.9646931
AGAGTAC100200.018.68056710-11
CATGGGG38350.017.8942664
GTATAGC8050.017.6689681
CTTTGCG6800.017.3997232
GAGTACA65100.017.1876641
ACTTTTT74750.016.93734616-17
CGATCGG850.001769957516.79100470-71
TACTTTT70500.016.74956114-15
CATGGGA36700.016.6354644
AGTACAT56600.016.4725592
TTGCGTT8400.016.339174
Produced by FastQC (version 0.11.5)