FastQCFastQC Report
Fri 27 May 2016
SRR938950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022595
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107800.5329786734368471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.31766122234060695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57490.2842388120211906No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53730.26564883231689984No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40530.2003861376103471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36700.18145006785837006No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34660.17136401504008464No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32970.16300841246023054No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27630.13660668596530695No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26410.1305748308484892No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25300.12508683152089273No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24590.12157648960864631No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23560.11648402176411986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72800.021.8580841
TACATGG71500.021.7018072
GAGTACT66550.021.14282612-13
GTACTTT69150.020.24492814-15
ACATGGG75050.020.1694563
TTTGCGT4300.019.8636133
CATGGGG39550.019.5566884
AGTACTT69900.018.77173412-13
AGAGTAC109800.018.4981310-11
ACTTTTT82850.017.0107516-17
GTATATA10100.016.9815831
TACTTTT78100.016.34510214-15
TACCGTG3553.02025E-816.0397037
GTACCGT2406.2057356E-515.8169316
TCTAGGA11750.015.7500132
GTCCTAC4058.742063E-915.2927431
GTATTAC5459.094947E-1214.8610481
GTATCAA223250.014.6822961
TAGATAG4603.1668606E-914.4419025
CATGGGA34050.014.2146864
Produced by FastQC (version 0.11.5)