##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938950_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2022595 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.132644449333654 33.0 31.0 34.0 30.0 34.0 2 32.3664703017658 34.0 31.0 34.0 30.0 34.0 3 32.4620672947377 34.0 31.0 34.0 30.0 34.0 4 36.044558104810896 37.0 35.0 37.0 35.0 37.0 5 35.95838909915233 37.0 35.0 37.0 35.0 37.0 6 35.994224745932826 37.0 35.0 37.0 35.0 37.0 7 35.93373413856951 37.0 35.0 37.0 35.0 37.0 8 35.901252598765446 37.0 35.0 37.0 35.0 37.0 9 37.569108002343526 39.0 37.0 39.0 35.0 39.0 10-11 37.48838867890012 39.0 37.0 39.0 34.5 39.0 12-13 37.41973108803295 39.0 37.0 39.0 34.0 39.0 14-15 38.664233818436216 40.0 38.0 41.0 34.0 41.0 16-17 38.665051332570286 40.0 38.0 41.0 34.0 41.0 18-19 38.75706110219792 40.0 38.0 41.0 34.0 41.0 20-21 38.74844889856843 40.0 38.0 41.0 34.0 41.0 22-23 38.688511540867054 40.0 38.0 41.0 34.0 41.0 24-25 38.623972421567345 40.0 38.0 41.0 34.0 41.0 26-27 38.46320098685105 40.0 38.0 41.0 34.0 41.0 28-29 38.28544518304455 40.0 38.0 41.0 34.0 41.0 30-31 38.12907403607741 40.0 37.5 41.0 33.0 41.0 32-33 37.98369965316833 40.0 37.0 41.0 33.0 41.0 34-35 37.96203688825494 40.0 37.0 41.0 33.0 41.0 36-37 37.86481574413068 40.0 37.0 41.0 33.0 41.0 38-39 37.744047869197736 40.0 37.0 41.0 33.0 41.0 40-41 37.59880722537137 40.0 37.0 41.0 32.5 41.0 42-43 37.4277771872273 40.0 37.0 41.0 32.0 41.0 44-45 37.17943063243012 40.0 36.0 41.0 31.0 41.0 46-47 36.88649729678952 40.0 36.0 41.0 31.0 41.0 48-49 36.596892358578955 39.0 36.0 41.0 30.0 41.0 50-51 36.324876211006156 39.0 35.0 41.0 30.0 41.0 52-53 36.09683278164931 39.0 35.0 40.5 29.5 41.0 54-55 35.84120869477083 39.0 35.0 40.0 28.5 41.0 56-57 35.53168825197333 38.0 35.0 40.0 28.0 41.0 58-59 35.20903517510921 38.0 34.0 40.0 28.0 41.0 60-61 34.76713405303583 37.5 34.0 40.0 26.5 41.0 62-63 34.44812876527431 37.0 34.0 40.0 26.0 41.0 64-65 34.18060981066402 36.5 33.5 39.0 26.0 41.0 66-67 33.69889078139717 36.0 33.0 39.0 26.0 40.5 68-69 33.2623983051476 35.5 32.5 39.0 25.0 40.0 70-71 32.78733038497573 35.0 32.0 38.0 24.5 40.0 72-73 32.26703442854353 35.0 31.5 37.0 22.5 39.0 74-75 31.80529270565783 35.0 31.0 36.5 21.5 39.0 76-77 29.85183168157738 33.0 28.5 35.0 19.5 37.0 78-79 30.840572136290263 34.0 30.5 35.0 20.0 37.0 80-81 30.848019746909294 34.0 31.0 35.0 20.0 37.0 82-83 30.533927454581864 34.0 30.5 35.0 18.5 37.0 84-85 30.135700918869077 34.0 30.0 35.0 17.0 36.0 86-87 29.589847942865475 34.0 29.5 35.0 9.0 36.0 88-89 29.02600322852573 34.0 29.0 35.0 4.5 35.0 90-91 28.563606653828373 33.5 29.0 35.0 2.0 35.0 92-93 28.201073620769357 33.0 28.5 35.0 2.0 35.0 94-95 27.791221920354793 33.0 27.0 35.0 2.0 35.0 96-97 27.147245494031182 33.0 26.5 35.0 2.0 35.0 98-99 26.235485107003626 33.0 25.0 34.0 2.0 35.0 100-101 21.882567197090864 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14403982151042527 1101 2 0.1667278519644526 1101 3 0.16482544940604527 1101 4 0.11115400548378318 1101 5 0.09579924480562596 1101 6 0.11639661118760358 1101 7 0.08446877316479373 1101 8 0.17097047384249464 1101 9 0.2470133569733406 1101 10-11 0.2400842988996743 1101 12-13 0.3327313540828527 1101 14-15 0.3252840864343085 1101 16-17 0.274483353255512 1101 18-19 0.34660061560759914 1101 20-21 0.2884212190842277 1101 22-23 0.3423388570492705 1101 24-25 0.27218857302440114 1101 26-27 0.2147004534640402 1101 28-29 0.25594488241048907 1101 30-31 0.262119095353448 1101 32-33 0.3347213904974353 1101 34-35 0.36215693593065623 1101 36-37 0.36034089407639414 1101 38-39 0.45890879104805293 1101 40-41 0.41512216896054355 1101 42-43 0.42332629622401186 1101 44-45 0.4038180861443905 1101 46-47 0.3757916664345018 1101 48-49 0.08016791286514291 1101 50-51 0.21555246279694273 1101 52-53 0.22699401079166392 1101 54-55 0.29362421816539097 1101 56-57 -0.02290491038439768 1101 58-59 0.012131826341850172 1101 60-61 0.20964055493237055 1101 62-63 0.09805307120952023 1101 64-65 0.19004872392780925 1101 66-67 0.1994894987952449 1101 68-69 0.20868550349562298 1101 70-71 0.3173901615858412 1101 72-73 -0.03441164998426416 1101 74-75 0.011474719965892888 1101 76-77 -0.007053840885976825 1101 78-79 0.21052050111872234 1101 80-81 0.3739972954301578 1101 82-83 0.2153939648994374 1101 84-85 0.29309961950445285 1101 86-87 0.4880003034339424 1101 88-89 0.748522051932536 1101 90-91 0.7217031778526461 1101 92-93 0.7990132198311457 1101 94-95 0.7286723434230211 1101 96-97 0.4407706019201463 1101 98-99 0.5052823814196437 1101 100-101 1.0024966929200723 1102 1 0.05556621029792552 1102 2 0.14084772094075504 1102 3 0.13768907925116025 1102 4 0.17596408894486615 1102 5 0.09076807568265366 1102 6 0.14760093888352088 1102 7 0.14195886277634884 1102 8 0.2468599496493482 1102 9 0.24712052429259046 1102 10-11 0.23635544567800082 1102 12-13 0.2681948838015984 1102 14-15 0.24054838848255145 1102 16-17 0.2956169298120699 1102 18-19 0.19726813340022176 1102 20-21 0.18928497732304095 1102 22-23 0.28074909975995155 1102 24-25 0.33073027431336044 1102 26-27 0.2920756462487262 1102 28-29 0.19953979719176118 1102 30-31 0.19751972251524563 1102 32-33 0.31953857702914235 1102 34-35 0.4152941944870818 1102 36-37 0.2571790180995279 1102 38-39 0.332155738203177 1102 40-41 0.26545355930377923 1102 42-43 0.16835647650842844 1102 44-45 0.3449568243346306 1102 46-47 0.4154743786892112 1102 48-49 0.30556628278292663 1102 50-51 0.3914700764965531 1102 52-53 0.31009903114050985 1102 54-55 0.2745665554173158 1102 56-57 0.2514262128171225 1102 58-59 0.16288388724188252 1102 60-61 0.04380158754903363 1102 62-63 0.18171347195316656 1102 64-65 0.11605823230937773 1102 66-67 0.11534283281196878 1102 68-69 0.16125425754351852 1102 70-71 0.21290124874393257 1102 72-73 0.3087861244559633 1102 74-75 0.20969051352954082 1102 76-77 0.0560740521786407 1102 78-79 0.03021886394378015 1102 80-81 0.2753440831803644 1102 82-83 0.13211188550139497 1102 84-85 0.29496559926484167 1102 86-87 0.3376549088588874 1102 88-89 0.4963895775944458 1102 90-91 0.43315180561912925 1102 92-93 0.48626520126169126 1102 94-95 0.47906826713024486 1102 96-97 0.20569170968853356 1102 98-99 0.297293005998025 1102 100-101 0.6820758566939986 1103 1 -0.030619955798357523 1103 2 -0.07165642122325977 1103 3 0.00743835761004874 1103 4 0.04127796540907269 1103 5 -0.00621465881458505 1103 6 -0.05867349272567424 1103 7 0.05458022199965029 1103 8 0.1149239887414808 1103 9 0.08409666256446968 1103 10-11 0.02190770774858919 1103 12-13 0.04543769804183739 1103 14-15 0.07572292944747261 1103 16-17 0.03993666608800339 1103 18-19 0.07734945908012492 1103 20-21 0.11274156080629183 1103 22-23 0.08055938669575369 1103 24-25 0.08373833789951846 1103 26-27 -0.07556187685042204 1103 28-29 -0.009607012033896467 1103 30-31 0.13327576734145907 1103 32-33 0.0905770608240104 1103 34-35 -0.056398523451910876 1103 36-37 -0.15307691492405695 1103 38-39 0.06464092467930271 1103 40-41 0.06076011328103448 1103 42-43 -0.008972265671566504 1103 44-45 0.042468220003769375 1103 46-47 0.1840008824216497 1103 48-49 0.10870019266557307 1103 50-51 0.20485390626915745 1103 52-53 0.15957025282965276 1103 54-55 0.2540163458817446 1103 56-57 0.13335429938333476 1103 58-59 0.13087982954807842 1103 60-61 0.06834975001608967 1103 62-63 0.024679706515883026 1103 64-65 0.1210279180463587 1103 66-67 -0.025209349864432795 1103 68-69 -0.061670567092939166 1103 70-71 -0.06452326104901118 1103 72-73 0.07571446222019063 1103 74-75 0.06241689694724428 1103 76-77 -0.14693714812873182 1103 78-79 -0.10672660994972105 1103 80-81 -0.03835797411829134 1103 82-83 0.08545140030138754 1103 84-85 0.215387502585628 1103 86-87 0.0578667950279268 1103 88-89 0.34163715860971067 1103 90-91 0.11519916568427035 1103 92-93 0.04623306353542134 1103 94-95 -0.08352168583455466 1103 96-97 -0.17498988811820837 1103 98-99 -0.09980548782914056 1103 100-101 0.16363362794033876 1104 1 -0.02815276894654062 1104 2 0.006081426230110765 1104 3 -0.004259639596597253 1104 4 0.017645764649614648 1104 5 0.04860294824806033 1104 6 -0.007202099396103279 1104 7 -0.047841574165836676 1104 8 0.040439054799577434 1104 9 9.553700453963643E-4 1104 10-11 0.09554458637039431 1104 12-13 0.021826830390907048 1104 14-15 0.0921453106027812 1104 16-17 0.027158391108862645 1104 18-19 0.11271474101386048 1104 20-21 0.0873788080025264 1104 22-23 -0.015106111190725358 1104 24-25 0.06163094034855732 1104 26-27 0.06151632518490402 1104 28-29 0.022077666116324224 1104 30-31 0.03415173562041218 1104 32-33 0.17063289188223507 1104 34-35 0.23333824655096436 1104 36-37 0.11516674824311934 1104 38-39 0.2846625096727209 1104 40-41 0.21442506421745122 1104 42-43 0.11236017458416114 1104 44-45 0.15061371488819475 1104 46-47 0.08457446031294324 1104 48-49 0.10945096387029452 1104 50-51 0.04256421088025064 1104 52-53 -0.06596889650879234 1104 54-55 0.09696804104138579 1104 56-57 -0.04746204414242783 1104 58-59 0.07802859533778417 1104 60-61 0.017035784746390448 1104 62-63 0.14001047507838393 1104 64-65 0.14493372234824164 1104 66-67 0.2069507738778782 1104 68-69 0.23020516832099247 1104 70-71 0.026548421793968657 1104 72-73 -0.013951163150892398 1104 74-75 -0.009234203404211883 1104 76-77 -0.18230255857517008 1104 78-79 0.012719777892360895 1104 80-81 0.12054014255376444 1104 82-83 0.2062488421789439 1104 84-85 0.08038478068660027 1104 86-87 -0.01696178187895825 1104 88-89 0.16709467667988065 1104 90-91 0.36423776216106774 1104 92-93 0.1271097052209278 1104 94-95 0.21102235880972842 1104 96-97 0.33939929357972787 1104 98-99 0.6100571680760432 1104 100-101 1.2224767668785042 1105 1 0.04613524838286054 1105 2 6.194838127768776E-4 1105 3 -0.07759107944323063 1105 4 -0.05204388021162032 1105 5 0.013050568505299509 1105 6 0.06072290252905077 1105 7 -0.01975079881303543 1105 8 0.00500268445357932 1105 9 -0.014521883612147235 1105 10-11 0.039451292813623695 1105 12-13 -0.024885203283822932 1105 14-15 0.016341670507934225 1105 16-17 0.017625368934062635 1105 18-19 5.451552774218271E-4 1105 20-21 -0.013146334534013704 1105 22-23 -0.11529622659006833 1105 24-25 -0.01090793530036649 1105 26-27 -0.05356524642238725 1105 28-29 0.05728951827634887 1105 30-31 -0.06261935283774989 1105 32-33 -0.1471808510309387 1105 34-35 -0.1073686007036656 1105 36-37 -0.09959139716665533 1105 38-39 0.012075930159774373 1105 40-41 0.10662408233791609 1105 42-43 0.03254745975426232 1105 44-45 0.08162737699482392 1105 46-47 0.1631758270236432 1105 48-49 0.050417932194726234 1105 50-51 0.16253217828560906 1105 52-53 0.18061707695505902 1105 54-55 0.06815395710058425 1105 56-57 0.0368337838196382 1105 58-59 0.06222631535311507 1105 60-61 -0.030986105026677535 1105 62-63 0.03766043472511171 1105 64-65 0.024514218368416607 1105 66-67 0.07378848773270619 1105 68-69 0.001468390641996109 1105 70-71 -0.025382784633748656 1105 72-73 0.06756543969393647 1105 74-75 0.17525173995720067 1105 76-77 -0.0564276127045531 1105 78-79 0.20755930989445304 1105 80-81 0.2668682451300697 1105 82-83 0.23041329949998612 1105 84-85 0.008508708724772163 1105 86-87 -0.15723839428456543 1105 88-89 0.015548901472090648 1105 90-91 0.2770894531480401 1105 92-93 0.33807654353483585 1105 94-95 0.40426576152535176 1105 96-97 0.11096454036471926 1105 98-99 0.40030217792184075 1105 100-101 0.9391928208788833 1106 1 -0.07540053195054242 1106 2 -0.16742019637530348 1106 3 -0.20698227785243972 1106 4 -0.14682341228744633 1106 5 -0.12770423862328073 1106 6 -0.12300425993075237 1106 7 -0.12411393607595045 1106 8 -0.07822786748130994 1106 9 -0.18040911453774555 1106 10-11 -0.09623110025844994 1106 12-13 -0.09973086089745209 1106 14-15 -0.24203957476958493 1106 16-17 -0.2407951643809767 1106 18-19 -0.27483370060627976 1106 20-21 -0.2195170296963056 1106 22-23 -0.040376128484318485 1106 24-25 -0.09682771083066655 1106 26-27 -0.11160596293888858 1106 28-29 -0.1302274193683104 1106 30-31 -0.22277734183515463 1106 32-33 -0.09946948260548538 1106 34-35 -0.10087537065286512 1106 36-37 -0.23575108515881738 1106 38-39 -0.11355906426691575 1106 40-41 -0.10265794154823027 1106 42-43 -0.1956351510440797 1106 44-45 -0.32774898683526743 1106 46-47 -0.1940492552379638 1106 48-49 -0.24285875338371454 1106 50-51 -0.14741090636295695 1106 52-53 -0.08812051933712439 1106 54-55 -0.019837380556133155 1106 56-57 -0.1686370571584419 1106 58-59 -0.09459583949965378 1106 60-61 -0.0721333303181595 1106 62-63 -0.2721878964212294 1106 64-65 -0.29815879860742456 1106 66-67 -0.20836153369935317 1106 68-69 -0.12086932786881732 1106 70-71 -0.1634483681669323 1106 72-73 -0.053476503387784646 1106 74-75 -0.040092561915049174 1106 76-77 -0.23306675098531215 1106 78-79 -0.20050583088808338 1106 80-81 -0.0339973566969789 1106 82-83 0.0910741997044795 1106 84-85 0.018746952357311386 1106 86-87 0.019382498777535062 1106 88-89 0.1536208100144023 1106 90-91 0.3048354437004228 1106 92-93 0.22455296852290374 1106 94-95 0.40034741836635845 1106 96-97 0.33191997678305185 1106 98-99 0.25594564299538547 1106 100-101 0.3804748418457997 1107 1 -0.05497495681469644 1107 2 -0.0858871779066277 1107 3 -0.08437973523567877 1107 4 -0.034823006010469726 1107 5 -0.10016204506615622 1107 6 -0.08153861572781551 1107 7 -0.08778431002005505 1107 8 -0.06203095857199514 1107 9 -0.0223504413706479 1107 10-11 -0.00762209106643752 1107 12-13 0.04037206260397852 1107 14-15 0.06071887663798492 1107 16-17 0.08611560703743493 1107 18-19 -0.00656816474386801 1107 20-21 -0.0021021362593742765 1107 22-23 -3.6631012397947416E-4 1107 24-25 -0.023514183500068953 1107 26-27 -0.012036785819056206 1107 28-29 -0.10128357023249634 1107 30-31 -0.1417327021520478 1107 32-33 -0.035999707919920354 1107 34-35 -0.032537889908702766 1107 36-37 -0.04042073119000378 1107 38-39 -0.19305815860781905 1107 40-41 -0.23018405330826397 1107 42-43 -0.12093181788002738 1107 44-45 -0.31882105021448126 1107 46-47 -0.5135420861421025 1107 48-49 -0.3943281217922916 1107 50-51 -0.3120529594159649 1107 52-53 -0.25308844038396217 1107 54-55 -0.5440150928781762 1107 56-57 -0.4744840600193072 1107 58-59 -0.31065055146621035 1107 60-61 -0.47124544583623873 1107 62-63 -0.4440075094424003 1107 64-65 -0.486761882926821 1107 66-67 -0.4160190083493589 1107 68-69 -0.3358643340476206 1107 70-71 -0.4388356009128529 1107 72-73 -0.3990083030255249 1107 74-75 -0.564296512958677 1107 76-77 -0.8089753725213775 1107 78-79 -0.2950397126953419 1107 80-81 -0.17155173856348682 1107 82-83 -0.22247767678731734 1107 84-85 -0.1444401212634645 1107 86-87 0.003101795241231997 1107 88-89 0.19003761633878113 1107 90-91 0.275409987062023 1107 92-93 0.2061613175148267 1107 94-95 0.18070392641572042 1107 96-97 0.27923093102610963 1107 98-99 0.01495991347428216 1107 100-101 0.6581634643975569 1108 1 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-1.7021798036129283 2316 86-87 -1.8361651946968394 2316 88-89 -1.9326036508394715 2316 90-91 -2.1646367917082046 2316 92-93 -2.6111620209400606 2316 94-95 -2.6826104757105114 2316 96-97 -2.8097225598342703 2316 98-99 -3.091282035056228 2316 100-101 -3.753575952601153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 9.0 5 26.0 6 91.0 7 233.0 8 539.0 9 1044.0 10 1758.0 11 2725.0 12 3750.0 13 5281.0 14 6495.0 15 8732.0 16 11203.0 17 15535.0 18 15550.0 19 11377.0 20 10634.0 21 11619.0 22 12734.0 23 14051.0 24 16227.0 25 19409.0 26 23302.0 27 28347.0 28 35013.0 29 42564.0 30 51825.0 31 62645.0 32 78213.0 33 99607.0 34 132767.0 35 191676.0 36 302314.0 37 459796.0 38 322549.0 39 22951.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.51263605288527 19.500375780476688 14.996619464932284 22.990368701705755 2 15.889686269371772 21.114162746372852 38.61657919652723 24.379571787728143 3 19.785770260482202 24.291368267003527 30.09945144727442 25.823410025239852 4 13.484657086564535 17.0182364734413 36.589529787228784 32.907576652765385 5 13.904737701870479 37.36299703745308 34.07309659326881 14.659168667407632 6 32.03048558905762 35.948422694607665 16.922122323055284 15.098969393279427 7 28.16312707190515 32.81477507855008 20.811877810436595 18.210220039108176 8 26.086586785787564 34.434525943157176 19.582219871007293 19.896667400047956 9 25.89223250329404 17.255555363283307 20.61065116842472 36.24156096499794 10-11 25.304645764475836 26.143716364373493 26.963405921600714 21.58823194954996 12-13 26.39302480229606 23.600621973257127 27.430454440953326 22.575898783493482 14-15 23.100244515701437 25.12600926692507 26.165751040940744 25.607995176432752 16-17 22.238620744212724 28.17726719867098 26.75217795445331 22.831934102662988 18-19 22.245061418623106 27.253256336537962 28.4633107468376 22.03837149800133 20-21 23.33630805969559 25.956259162115998 28.275828823862415 22.431603954326 22-23 22.84995265982562 25.88506349516339 28.386651801275093 22.878332043735895 24-25 23.125736986396188 26.05264524039662 28.34984265263189 22.471775120575302 26-27 22.777372632682273 25.901305006686954 28.95705764129744 22.36426471933333 28-29 22.410541902852525 26.81809754300787 28.49243175227863 22.278928801860975 30-31 22.750624816139663 26.077489561676952 28.18169233089175 22.990193291291632 32-33 22.63742864982856 26.224825039120535 28.390261026058113 22.747485284992795 34-35 23.278816264838344 26.1311603636405 28.151261750654 22.438761620867155 36-37 23.070934489166902 25.784987563268363 28.678848507728176 22.465229439836556 38-39 22.592577300318172 26.384898360722207 28.52145069767522 22.501073641284393 40-41 22.872766736957644 26.379141131594103 28.252894249571703 22.495197881876546 42-43 22.606497641626042 26.358611278663886 27.747975358205856 23.28691572150422 44-45 22.88041716901461 26.086264338392617 27.925247165076584 23.108071327516193 46-47 23.444715384448045 26.539710509505642 27.581123787939017 22.434450318107295 48-49 23.094465334891403 26.459064708416964 27.60521974687387 22.841250209817765 50-51 22.566083781934395 26.777280943585037 27.946997504182768 22.709637770297796 52-53 23.06353966068343 27.04068288510552 26.96973442532984 22.926043028881217 54-55 22.805504809415627 26.615189892192948 27.191059010825203 23.38824628756622 56-57 23.01456791893582 26.65303731097921 27.16322348270415 23.169171287380816 58-59 23.40456301417713 26.486603676427507 27.140592364685155 22.968240944710207 60-61 22.909018992231296 26.692657120078117 27.75431852314234 22.64400536454825 62-63 22.545492300732477 26.660231039827547 27.84121388612154 22.953062773318436 64-65 22.444275573807207 27.134506451391218 27.312471601963228 23.10874637283835 66-67 22.787805763185503 26.729835723206506 26.94013829983951 23.542220213768477 68-69 22.471252362417385 26.62609097639993 27.49575722816286 23.406899433019827 70-71 23.13708379581676 26.379947542637055 27.14134564754684 23.341623013999342 72-73 22.803749638459504 26.294537463011626 27.477942939639423 23.423769958889444 74-75 22.41078911002944 27.34390720831407 26.874707986522267 23.37059569513422 76-77 22.65690378122464 26.49074958931478 27.03599500915673 23.816351620303852 78-79 22.68911177653839 26.64613527137312 27.225366837182065 23.43938611490643 80-81 22.93437391074338 26.58831847206188 27.005628907418437 23.471678709776302 82-83 22.73992074537908 26.39999604468517 27.46671973380731 23.39336347612844 84-85 22.493949604344913 26.686286676274783 27.171900454614 23.647863264766304 86-87 22.47509819853409 26.726241846909808 27.153448175980486 23.64521177857561 88-89 22.380529688029565 26.68375764904226 26.806837551660905 24.128875111267263 90-91 22.540110984223492 26.29016927425684 27.03652974379174 24.13318999772793 92-93 22.744568240305153 26.508149184587126 26.953443472370893 23.79383910273683 94-95 22.489104343677305 26.299160237220008 27.104561219621324 24.10717419948136 96-97 21.98480064630132 26.238637071550976 27.57262237551333 24.20393990663438 98-99 22.169242977462122 26.54236265787268 26.872804491259988 24.415589873405207 100-101 22.217478579445167 25.73349022787614 27.483498880148723 24.565532312529974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 566.0 1 418.5 2 308.5 3 357.5 4 315.5 5 311.0 6 538.0 7 1121.0 8 1823.0 9 2169.0 10 2004.0 11 1807.0 12 1869.0 13 1966.5 14 2128.0 15 2284.0 16 2437.5 17 2612.5 18 2760.5 19 2941.0 20 3264.0 21 3883.5 22 4737.5 23 5756.5 24 6842.0 25 8294.5 26 10276.5 27 12650.5 28 14869.0 29 17248.5 30 19999.0 31 23159.0 32 26700.0 33 29654.0 34 32501.0 35 36000.0 36 40612.0 37 44752.5 38 47744.5 39 50170.5 40 52091.5 41 53780.0 42 55003.0 43 57538.0 44 61585.0 45 64899.0 46 68685.0 47 80246.0 48 98035.0 49 97630.0 50 83465.5 51 77656.5 52 76868.0 53 76735.5 54 75344.0 55 70796.0 56 65580.0 57 59782.0 58 54332.0 59 49384.0 60 41791.0 61 33982.0 62 27102.5 63 20368.5 64 14750.0 65 10657.5 66 7373.0 67 5460.0 68 4088.5 69 2923.0 70 2031.5 71 1170.5 72 728.0 73 465.5 74 273.0 75 173.5 76 99.5 77 52.5 78 29.0 79 18.5 80 13.5 81 8.0 82 7.5 83 8.0 84 6.0 85 3.0 86 1.0 87 2.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4015138967514505 2 0.0 3 0.0 4 0.0 5 5.438557892212726E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012607566022856775 16-17 0.0027192789461063633 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0023731888984200988 36-37 0.007836467508324702 38-39 0.013423349706688684 40-41 0.002472071769187603 42-43 7.41621530756281E-4 44-45 4.6969363614564455E-4 46-47 3.4609004768626446E-4 48-49 3.2136932999438843E-4 50-51 5.438557892212726E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.2136932999438843E-4 60-61 0.0028428825345657434 62-63 0.0 64-65 3.955314830700165E-4 66-67 0.0016315673676638178 68-69 0.0018540538268907022 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12338110200015327 78-79 7.416215307562809E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14644553160667362 88-89 0.43817472108850264 90-91 0.33684449926950283 92-93 0.0 94-95 0.0 96-97 4.449729184537685E-4 98-99 0.0 100-101 2.472071769187603E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2022595.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.982703462379064 #Duplication Level Percentage of deduplicated Percentage of total 1 71.4343965418444 34.276154662813155 2 14.39886187244848 13.817926388429031 3 5.501070433001738 7.918686939371508 4 2.6755031734191035 5.135115015312921 5 1.4877254290021529 3.5692544046625487 6 0.9888390629777939 2.846830291852816 7 0.6550001472468454 2.2000074483212004 8 0.47082646262750866 1.8073221230797318 9 0.3595146122866148 1.5525434728566736 >10 1.8711040191399206 15.99174390450898 >50 0.1134911763337677 3.686324267721816 >100 0.0399519060740031 3.3486373384821695 >500 0.0015479848323502448 0.5183509149513914 >1k 0.001754382809996941 1.9284159373201146 >5k 3.095969664700484E-4 0.8688864639522405 >10k+ 1.0319898882334948E-4 0.5338004263637744 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10780 0.5329786734368471 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6425 0.31766122234060695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5749 0.2842388120211906 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5373 0.26564883231689984 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4053 0.2003861376103471 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3670 0.18145006785837006 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3466 0.17136401504008464 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3297 0.16300841246023054 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2763 0.13660668596530695 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2641 0.1305748308484892 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2530 0.12508683152089273 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2459 0.12157648960864631 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2356 0.11648402176411986 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.9441435383752064E-5 0.0 0.0 0.0 0.0 3 4.9441435383752064E-5 0.0 0.0 0.0 0.0 4 4.9441435383752064E-5 0.0 0.0 0.0 0.0 5 9.888287076750413E-5 0.0 0.0 0.0 0.0 6 9.888287076750413E-5 0.0 0.0 0.0 0.0 7 9.888287076750413E-5 0.0 0.0 0.0 0.0 8 9.888287076750413E-5 0.0 0.0 0.0 0.0 9 9.888287076750413E-5 0.0 0.0 0.0 0.0 10-11 9.888287076750413E-5 0.0 0.0 2.4720717691876032E-5 0.0 12-13 1.4832430615125617E-4 0.0 0.0 4.9441435383752064E-5 2.472071769187603E-4 14-15 1.4832430615125617E-4 0.0 0.0 4.9441435383752064E-5 3.9553148307001646E-4 16-17 1.4832430615125617E-4 0.0 0.0 7.41621530756281E-5 4.449729184537686E-4 18-19 1.730450238431322E-4 0.0 0.0 9.888287076750413E-5 4.449729184537686E-4 20-21 2.472071769187603E-4 0.0 0.0 1.2360358845938014E-4 4.944143538375206E-4 22-23 2.472071769187603E-4 0.0 0.0 3.460900476862644E-4 4.944143538375206E-4 24-25 2.472071769187603E-4 0.0 0.0 6.180179422969007E-4 4.944143538375206E-4 26-27 2.472071769187603E-4 0.0 0.0 0.0013596394730531816 5.191350715293966E-4 28-29 3.460900476862644E-4 0.0 0.0 0.004276684160694553 5.438557892212726E-4 30-31 3.955314830700165E-4 0.0 0.0 0.01176706162133299 5.438557892212726E-4 32-33 3.955314830700165E-4 0.0 0.0 0.028750194675651822 5.438557892212726E-4 34-35 3.955314830700165E-4 0.0 0.0 0.05441029963981914 5.438557892212726E-4 36-37 3.955314830700165E-4 0.0 0.0 0.09319710569837264 5.438557892212726E-4 38-39 4.449729184537686E-4 0.0 0.0 0.14968394562430937 5.932972246050247E-4 40-41 4.449729184537686E-4 0.0 0.0 0.21333979368089012 5.932972246050247E-4 42-43 4.944143538375206E-4 0.0 0.0 0.28189034384046235 6.427386599887767E-4 44-45 4.944143538375206E-4 0.0 0.0 0.36848701791510413 6.921800953725288E-4 46-47 4.944143538375206E-4 0.0 0.0 0.4737478338471123 7.416215307562809E-4 48-49 5.932972246050247E-4 0.0 0.0 0.5825189916913668 8.405044015237851E-4 50-51 5.932972246050247E-4 0.0 0.0 0.7008817880000692 9.393872722912892E-4 52-53 5.932972246050247E-4 0.0 0.0 0.8214694489010406 0.0010382701430587933 54-55 5.932972246050247E-4 0.0 0.0 0.9589166392678712 0.0010629908607506693 56-57 5.932972246050247E-4 0.0 0.0 1.1240263127319112 0.0010877115784425453 58-59 5.932972246050247E-4 0.0 0.0 1.3041661825526119 0.0010877115784425453 60-61 5.932972246050247E-4 0.0 0.0 1.4951831681577379 0.0011371530138262974 62-63 5.932972246050247E-4 0.0 0.0 1.69220728816199 0.0011371530138262974 64-65 5.932972246050247E-4 0.0 0.0 1.9074505771051546 0.0011865944492100494 66-67 5.932972246050247E-4 0.0 0.0 2.1528778623501 0.0011865944492100494 68-69 5.932972246050247E-4 0.0 0.0 2.4315792336083097 0.0011865944492100494 70-71 5.932972246050247E-4 0.0 0.0 2.741848961359046 0.0012113151669019256 72-73 6.180179422969007E-4 0.0 0.0 3.0570628326481577 0.0012360358845938015 74-75 6.674593776806528E-4 0.0 0.0 3.3969479801937608 0.0012360358845938015 76-77 6.921800953725288E-4 0.0 0.0 3.756906350505168 0.0012360358845938015 78-79 6.921800953725288E-4 0.0 0.0 4.179111487964718 0.0012360358845938015 80-81 6.921800953725288E-4 0.0 0.0 4.639485413540526 0.0013101980376694295 82-83 7.169008130644048E-4 0.0 0.0 5.102306690167829 0.0013843601907450576 84-85 7.416215307562809E-4 0.0 0.0 5.580875063964857 0.0013843601907450576 86-87 7.66342248448157E-4 0.0 0.0 6.08599348856296 0.0014585223438206858 88-89 7.91062966140033E-4 0.0 0.0 6.653754211792277 0.001532684496896314 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7280 0.0 21.858084 1 TACATGG 7150 0.0 21.701807 2 GAGTACT 6655 0.0 21.142826 12-13 GTACTTT 6915 0.0 20.244928 14-15 ACATGGG 7505 0.0 20.169456 3 TTTGCGT 430 0.0 19.863613 3 CATGGGG 3955 0.0 19.556688 4 AGTACTT 6990 0.0 18.771734 12-13 AGAGTAC 10980 0.0 18.49813 10-11 ACTTTTT 8285 0.0 17.01075 16-17 GTATATA 1010 0.0 16.981583 1 TACTTTT 7810 0.0 16.345102 14-15 TACCGTG 355 3.02025E-8 16.039703 7 GTACCGT 240 6.2057356E-5 15.816931 6 TCTAGGA 1175 0.0 15.750013 2 GTCCTAC 405 8.742063E-9 15.292743 1 GTATTAC 545 9.094947E-12 14.861048 1 GTATCAA 22325 0.0 14.682296 1 TAGATAG 460 3.1668606E-9 14.441902 5 CATGGGA 3405 0.0 14.214686 4 >>END_MODULE