##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938949_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 316239 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.609440960792313 33.0 31.0 34.0 30.0 34.0 2 31.534510291267047 33.0 31.0 34.0 30.0 34.0 3 31.631990361720092 33.0 31.0 34.0 28.0 34.0 4 35.00205224529549 37.0 35.0 37.0 32.0 37.0 5 35.090823712445335 37.0 35.0 37.0 33.0 37.0 6 35.33077514158595 37.0 35.0 37.0 33.0 37.0 7 35.336438579681825 37.0 35.0 37.0 33.0 37.0 8 35.38748857667777 37.0 35.0 37.0 33.0 37.0 9 36.79270741432904 39.0 37.0 39.0 33.0 39.0 10-11 36.67313645692024 39.0 37.0 39.0 32.5 39.0 12-13 36.706502044339885 39.0 37.0 39.0 32.0 39.0 14-15 37.502956624578246 40.0 37.0 41.0 32.0 41.0 16-17 37.57574650817894 39.5 37.0 41.0 32.5 41.0 18-19 37.60189919649379 40.0 37.0 41.0 32.0 41.0 20-21 37.74477531234288 40.0 37.0 41.0 32.0 41.0 22-23 37.71707948734976 40.0 37.5 41.0 32.5 41.0 24-25 37.47318009480172 40.0 37.0 41.0 32.0 41.0 26-27 37.20008442981416 39.5 36.5 41.0 31.5 41.0 28-29 37.226833186292644 39.5 36.5 41.0 31.5 41.0 30-31 37.3740398875534 40.0 37.0 41.0 31.5 41.0 32-33 37.08464167923627 40.0 36.0 41.0 31.0 41.0 34-35 36.58812796650634 39.0 35.0 41.0 30.0 41.0 36-37 36.15085109679704 38.5 35.0 40.5 29.5 41.0 38-39 35.86103073940912 38.0 35.0 40.0 30.0 41.0 40-41 36.15050009644604 39.0 35.0 40.0 30.0 41.0 42-43 35.94212605023416 39.0 35.0 40.0 30.0 41.0 44-45 35.957544768355575 39.0 35.0 40.5 30.0 41.0 46-47 35.69566846593874 39.0 35.0 40.0 28.0 41.0 48-49 35.29590910671992 38.5 34.5 40.0 26.5 41.0 50-51 34.014572838897166 37.0 33.5 39.0 24.5 40.5 52-53 34.402760254111605 37.5 33.5 39.5 25.0 40.0 54-55 35.12614035587009 38.0 34.5 40.0 26.5 41.0 56-57 34.77797014283501 38.0 34.0 40.0 24.5 41.0 58-59 34.5991449504963 38.0 34.0 40.0 24.5 41.0 60-61 33.762853411502064 38.0 32.0 40.0 22.0 41.0 62-63 33.57349662755068 37.0 32.5 40.0 22.0 41.0 64-65 33.113147018552425 36.5 32.0 39.0 22.0 40.5 66-67 32.26685196955467 36.0 31.0 39.0 18.5 39.0 68-69 31.596676880460663 35.5 30.5 37.5 16.0 39.0 70-71 30.55880204528853 34.5 29.5 36.5 10.5 38.0 72-73 29.9628508817698 34.0 29.0 36.0 7.5 37.0 74-75 29.562032197167333 34.0 29.0 35.5 4.5 37.0 76-77 29.227851403527076 34.0 29.0 35.0 2.0 36.5 78-79 28.64696795777877 34.0 29.0 35.0 2.0 36.0 80-81 27.990209936155882 33.0 26.0 35.0 2.0 36.0 82-83 27.392367797773204 32.5 25.5 35.0 2.0 35.0 84-85 26.880511258889637 32.0 25.0 35.0 2.0 35.0 86-87 26.803828749774695 33.0 25.0 35.0 2.0 35.0 88-89 26.071940842211113 32.0 24.5 34.5 2.0 35.0 90-91 25.38700160321782 32.0 20.5 34.0 2.0 35.0 92-93 24.655289195829738 31.0 18.5 34.0 2.0 35.0 94-95 23.912251493332576 31.0 8.5 34.0 2.0 35.0 96-97 22.960039716796473 31.0 2.0 34.0 2.0 35.0 98-99 22.15055701542188 31.0 2.0 34.0 2.0 35.0 100-101 18.992467722197453 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5497192554364183 1101 2 0.4828358983902419 1101 3 0.7379998551754525 1101 4 0.7383563141220648 1101 5 0.3286050542183858 1101 6 0.42243309361540327 1101 7 0.5411132484997268 1101 8 0.5914772808130877 1101 9 0.8852185407031854 1101 10-11 0.8619751809252421 1101 12-13 0.7349241387469476 1101 14-15 0.8885811265004335 1101 16-17 0.7647003637829783 1101 18-19 0.7593135144556129 1101 20-21 1.1325748142619148 1101 22-23 0.9822055359701594 1101 24-25 0.8505183201037951 1101 26-27 1.1622035293587416 1101 28-29 1.0192043521365335 1101 30-31 0.8451796824415467 1101 32-33 0.8580918862302482 1101 34-35 0.9261741814433222 1101 36-37 0.9667934205836914 1101 38-39 1.2205283465452368 1101 40-41 0.813753887861246 1101 42-43 1.3187394542275115 1101 44-45 1.1887909476911105 1101 46-47 1.2720796014092457 1101 48-49 1.2443985549423715 1101 50-51 1.4195684448266874 1101 52-53 1.3401126789394056 1101 54-55 1.4658280783039501 1101 56-57 1.5225654536664308 1101 58-59 1.0818641972684944 1101 60-61 1.567345415631408 1101 62-63 1.451218643060237 1101 64-65 1.365798002634321 1101 66-67 1.7130268112186045 1101 68-69 1.5459075072390398 1101 70-71 1.6045490768069364 1101 72-73 1.4931400793455971 1101 74-75 1.2482988407128808 1101 76-77 1.152400405393216 1101 78-79 1.0527745830721678 1101 80-81 1.3529145424055287 1101 82-83 1.4807106229257627 1101 84-85 1.520190678279107 1101 86-87 1.485032990983015 1101 88-89 1.379389163324685 1101 90-91 1.421372816953042 1101 92-93 1.9161694525662298 1101 94-95 1.2318406192395805 1101 96-97 1.9804203253583772 1101 98-99 1.8131457657255226 1101 100-101 2.0600265902049024 1102 1 0.1517953800038896 1102 2 0.33750717866706026 1102 3 0.5649894745526112 1102 4 0.38541513765147783 1102 5 0.491234812003853 1102 6 0.6265853427503529 1102 7 0.679521552997997 1102 8 0.45652918392727315 1102 9 0.5322773642325984 1102 10-11 0.6647433470152109 1102 12-13 0.7003220626223907 1102 14-15 0.7761243790955845 1102 16-17 0.8719667997691403 1102 18-19 0.8544692237981764 1102 20-21 1.0374191049193513 1102 22-23 0.9026207608836572 1102 24-25 0.7086498079930621 1102 26-27 0.8196429757255217 1102 28-29 0.5832181929669886 1102 30-31 0.29327656825469717 1102 32-33 0.5120711249845726 1102 34-35 0.6303264305782648 1102 36-37 0.7937830399608501 1102 38-39 0.589040457271885 1102 40-41 0.6528542338820031 1102 42-43 0.17341073450432987 1102 44-45 0.7458843732966471 1102 46-47 0.5471661065995619 1102 48-49 0.5783085895444486 1102 50-51 0.26212899845990734 1102 52-53 0.3937458969324865 1102 54-55 0.367212161348931 1102 56-57 0.4187592252927317 1102 58-59 0.17182959519237073 1102 60-61 0.32513088275943147 1102 62-63 0.2816684700498584 1102 64-65 0.5734104593817264 1102 66-67 0.5746185067203342 1102 68-69 0.44210127886533357 1102 70-71 0.43672900760278566 1102 72-73 0.20421274370545817 1102 74-75 0.4593715050727418 1102 76-77 0.22160455764234754 1102 78-79 -0.0769832023949597 1102 80-81 0.4030875527861504 1102 82-83 0.5170428028565581 1102 84-85 0.3714017509434626 1102 86-87 0.16842399444322353 1102 88-89 0.24790127405132978 1102 90-91 0.7518226439426599 1102 92-93 0.2500794871683034 1102 94-95 0.231840619239577 1102 96-97 -0.24449316945131372 1102 98-99 0.15224611174628322 1102 100-101 0.39566672861320384 1103 1 0.2879244351192085 1103 2 0.14798700199570902 1103 3 0.24806752408199984 1103 4 0.34253454433806496 1103 5 -0.032343019048262533 1103 6 0.057044617405807685 1103 7 0.3651504723031209 1103 8 0.20917748275694947 1103 9 0.7698743504271945 1103 10-11 0.4279139836697894 1103 12-13 0.3035614310459209 1103 14-15 0.299365637052297 1103 16-17 0.46323715653662134 1103 18-19 0.5836814065632723 1103 20-21 0.6769611293179452 1103 22-23 0.7335429711650789 1103 24-25 0.7317670653356743 1103 26-27 0.7084380440439588 1103 28-29 0.646800984043665 1103 30-31 0.6356009302420134 1103 32-33 0.7253167524867266 1103 34-35 0.6270669858648219 1103 36-37 0.7330581677995838 1103 38-39 0.8492055552323734 1103 40-41 0.6366810109289034 1103 42-43 0.5692502186617716 1103 44-45 0.6522048617409624 1103 46-47 0.6082334566473904 1103 48-49 0.7315320150318954 1103 50-51 0.6589486779478406 1103 52-53 0.578559955059248 1103 54-55 0.6632665799041533 1103 56-57 0.19638479067648262 1103 58-59 0.8500302872338921 1103 60-61 0.6649870420470805 1103 62-63 0.8446607820117862 1103 64-65 1.175693251639366 1103 66-67 0.6609819820442127 1103 68-69 0.662810562377981 1103 70-71 0.5776114195918716 1103 72-73 1.0541956198111322 1103 74-75 0.9473139215494726 1103 76-77 1.094356308412923 1103 78-79 1.034635064666933 1103 80-81 1.0429956742358968 1103 82-83 0.820678973245208 1103 84-85 0.8532149942090506 1103 86-87 1.5505348391826423 1103 88-89 0.8906968383722216 1103 90-91 0.840666131548506 1103 92-93 0.7765979042118936 1103 94-95 1.1608414104815914 1103 96-97 1.4735943867918238 1103 98-99 1.3818775346828538 1103 100-101 1.349018878547664 1104 1 0.3527708515014609 1104 2 0.4380893910742074 1104 3 0.2719583090649351 1104 4 0.6428758412663882 1104 5 0.39769111064457263 1104 6 0.5041435935150176 1104 7 0.5221470593338395 1104 8 0.43443345545318834 1104 9 0.7528095040108127 1104 10-11 0.7879822430554597 1104 12-13 0.7574863457216878 1104 14-15 0.85226666094308 1104 16-17 0.8762064398130747 1104 18-19 0.5341933519557642 1104 20-21 0.5728655661780238 1104 22-23 0.8188672032469881 1104 24-25 0.752244881035331 1104 26-27 0.43710698602347975 1104 28-29 0.3464596871153347 1104 30-31 0.7772391554979805 1104 32-33 0.6928935442956075 1104 34-35 0.852322958561075 1104 36-37 0.6921025364002631 1104 38-39 0.7058608453347617 1104 40-41 0.9711520006899974 1104 42-43 1.0095232562044387 1104 44-45 1.1146621996249237 1104 46-47 0.9205201460671901 1104 48-49 1.093306759059196 1104 50-51 0.7459793946714015 1104 52-53 1.0939183168339923 1104 54-55 1.1137785252966381 1104 56-57 0.8448489544990139 1104 58-59 1.1008835295547073 1104 60-61 1.1974102502382067 1104 62-63 1.0955140243326085 1104 64-65 1.0272290878168349 1104 66-67 1.2155894905766402 1104 68-69 1.0706603917295148 1104 70-71 1.2141301909229334 1104 72-73 1.1582911829510643 1104 74-75 0.9148907133583464 1104 76-77 1.2871890729180429 1104 78-79 1.1165463274655671 1104 80-81 1.2597192237239483 1104 82-83 1.3531021814363378 1104 84-85 1.6757405914786716 1104 86-87 1.6648693101724028 1104 88-89 1.950423800829558 1104 90-91 1.8287207390570366 1104 92-93 1.83632486666923 1104 94-95 1.4577697381266432 1104 96-97 2.3592599158020633 1104 98-99 3.156621561986608 1104 100-101 2.4770052266705314 1105 1 0.416726928070684 1105 2 0.277896712406978 1105 3 0.5778813517441748 1105 4 0.4592946205876771 1105 5 0.40086437305510714 1105 6 0.3266235323326967 1105 7 0.5080271830682435 1105 8 0.5712946651738733 1105 9 0.507326837100635 1105 10-11 0.5159891374817036 1105 12-13 0.5050807160345698 1105 14-15 0.7026267919828371 1105 16-17 0.9977378242914767 1105 18-19 0.9293786681976144 1105 20-21 0.9578690476349863 1105 22-23 0.7509502559710839 1105 24-25 0.6239788748942701 1105 26-27 0.9727947735290456 1105 28-29 0.906209478798651 1105 30-31 1.3359273881734595 1105 32-33 1.2987012993836657 1105 34-35 1.3313714364224296 1105 36-37 1.1132446825758038 1105 38-39 1.005665655452674 1105 40-41 1.2628980940386967 1105 42-43 1.0185693369970679 1105 44-45 1.406853462880143 1105 46-47 1.616503343756868 1105 48-49 1.4177271820847537 1105 50-51 1.3605250360014267 1105 52-53 1.4376465577279873 1105 54-55 1.3567100262041052 1105 56-57 1.4007348996860358 1105 58-59 1.2329734310466023 1105 60-61 1.4503637813487273 1105 62-63 1.5346513078830029 1105 64-65 1.454420978875767 1105 66-67 1.4350240106492365 1105 68-69 1.2202603095443045 1105 70-71 1.38471016740883 1105 72-73 1.0908764786466136 1105 74-75 1.018461204300852 1105 76-77 0.9734127765033733 1105 78-79 1.1253926525309161 1105 80-81 1.1822939038294216 1105 82-83 1.3294625179391275 1105 84-85 1.188051251585744 1105 86-87 1.6279430371576922 1105 88-89 1.5450509424963172 1105 90-91 1.5514141311375766 1105 92-93 1.7456471810961602 1105 94-95 1.2702790124996959 1105 96-97 0.9536644725155661 1105 98-99 0.9263431595758433 1105 100-101 1.1526247547904305 1106 1 0.4464970748444337 1106 2 0.3405045559619282 1106 3 0.3058706153191473 1106 4 0.5867683653419533 1106 5 0.47833066195867247 1106 6 0.5335233896064082 1106 7 0.27922842713019236 1106 8 0.22814446173566694 1106 9 0.6416706157257792 1106 10-11 0.7421517176320904 1106 12-13 0.5938159907294107 1106 14-15 0.7444700913032918 1106 16-17 0.637934445725989 1106 18-19 0.6840705285388822 1106 20-21 0.5884902265114889 1106 22-23 0.6530229824691816 1106 24-25 0.541320118530173 1106 26-27 0.610983640878807 1106 28-29 0.43651751194523314 1106 30-31 0.22363162522690772 1106 32-33 0.4953706643582123 1106 34-35 0.13520267856716117 1106 36-37 0.0925014407719118 1106 38-39 0.2703377045334747 1106 40-41 0.59953507931872 1106 42-43 0.5309140955340155 1106 44-45 0.6171404066204289 1106 46-47 0.4757635103418778 1106 48-49 0.283612404588645 1106 50-51 0.06404313241622361 1106 52-53 0.4003246993039369 1106 54-55 0.44173335236561684 1106 56-57 0.31295290876087734 1106 58-59 0.45353347194726723 1106 60-61 0.34698308561134894 1106 62-63 0.40859417476411153 1106 64-65 0.5688097056294907 1106 66-67 0.5870488066784603 1106 68-69 0.6138333662300717 1106 70-71 0.3988816093703562 1106 72-73 0.19794249487906512 1106 74-75 0.3150070151778728 1106 76-77 0.48665756273348393 1106 78-79 0.3578128216722156 1106 80-81 0.08766318146399854 1106 82-83 0.25839228382541535 1106 84-85 0.5822154507208666 1106 86-87 0.11982681109629922 1106 88-89 0.7196190357157555 1106 90-91 0.3264870919624059 1106 92-93 -0.031172579038472037 1106 94-95 -0.209297282155525 1106 96-97 0.06481522981697552 1106 98-99 0.4767287335698356 1106 100-101 0.9978199915669457 1107 1 0.12734702785686025 1107 2 0.3371214506053448 1107 3 -0.03314995479675531 1107 4 0.12228052990288063 1107 5 0.2662078677642441 1107 6 0.12002229322967395 1107 7 -0.19734043577050642 1107 8 0.2470334666432521 1107 9 0.1993713570852833 1107 10-11 0.21043510491412576 1107 12-13 0.23637629144988637 1107 14-15 0.32936619655546195 1107 16-17 0.14563518708548884 1107 18-19 0.2073763654397922 1107 20-21 0.33920026096904365 1107 22-23 0.4283284017027569 1107 24-25 0.0187921870276071 1107 26-27 0.2447833693950372 1107 28-29 0.014428131137044886 1107 30-31 0.1513229690715292 1107 32-33 0.21837891328794257 1107 34-35 0.38829341679416274 1107 36-37 0.2932271377542648 1107 38-39 0.7084289648446358 1107 40-41 0.3480836853540197 1107 42-43 0.5764398342831072 1107 44-45 0.49738474200729144 1107 46-47 0.8392019566194193 1107 48-49 1.2113507360076667 1107 50-51 1.221383635705358 1107 52-53 1.0203100809474677 1107 54-55 0.8113950418299609 1107 56-57 0.8626187748984933 1107 58-59 0.6647843855945439 1107 60-61 0.2736929112352442 1107 62-63 0.41716679667076306 1107 64-65 0.7074282709422235 1107 66-67 0.28680864796488237 1107 68-69 0.16725301747785082 1107 70-71 0.2256279808854451 1107 72-73 0.531032188937747 1107 74-75 0.5261535119931615 1107 76-77 0.43099817043944455 1107 78-79 0.6879892861182846 1107 80-81 0.7833246620946781 1107 82-83 0.8477365461204975 1107 84-85 0.926107066049255 1107 86-87 1.1236171420976575 1107 88-89 1.1293976720319279 1107 90-91 1.4637064717264252 1107 92-93 1.0978814044636707 1107 94-95 0.6721321842744636 1107 96-97 1.2918957351944407 1107 98-99 1.9969281697189416 1107 100-101 2.042646137541677 1108 1 -0.1929764983617943 1108 2 -0.21703119297298556 1108 3 -0.16941094708857918 1108 4 -0.11485984573494079 1108 5 -0.05786118011329222 1108 6 0.012031847024047693 1108 7 0.13197234242947076 1108 8 0.20722237121387366 1108 9 -0.15480299966208122 1108 10-11 0.10176069388383269 1108 12-13 0.19472500494461542 1108 14-15 -0.09430115769489333 1108 16-17 0.1725866581526958 1108 18-19 0.05485763783149622 1108 20-21 0.07509755767949855 1108 22-23 0.15177862426274658 1108 24-25 0.04685645800795868 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58-59 -2.729289826080553 2316 60-61 -2.761991851686787 2316 62-63 -3.6353061518268888 2316 64-65 -3.5639784071797322 2316 66-67 -3.7231946028594365 2316 68-69 -3.3987340721055794 2316 70-71 -2.7305323311936895 2316 72-73 -3.0132951226305416 2316 74-75 -3.4925433718595293 2316 76-77 -3.8960719080447426 2316 78-79 -3.3708459899695775 2316 80-81 -3.506574562351524 2316 82-83 -2.943449075947953 2316 84-85 -3.4282002761696617 2316 86-87 -3.2555543621051974 2316 88-89 -3.908116840576625 2316 90-91 -4.068295571648857 2316 92-93 -3.7773473142471445 2316 94-95 -3.538256521433052 2316 96-97 -4.118729816284681 2316 98-99 -4.45395187486379 2316 100-101 -3.7068406966198424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1883.0 3 323.0 4 316.0 5 297.0 6 327.0 7 362.0 8 419.0 9 522.0 10 711.0 11 911.0 12 1328.0 13 1867.0 14 1617.0 15 1696.0 16 2036.0 17 2348.0 18 2290.0 19 2192.0 20 2401.0 21 2820.0 22 3011.0 23 3534.0 24 4122.0 25 4683.0 26 5352.0 27 6192.0 28 7326.0 29 8689.0 30 10362.0 31 12533.0 32 15346.0 33 19404.0 34 25689.0 35 36672.0 36 48836.0 37 53760.0 38 23805.0 39 257.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.49809972780743 17.247746004352543 14.264413834235354 12.98974043360468 2 13.75319262065495 29.58447666340679 25.994182863183347 30.668147852754913 3 37.827281474729055 20.329750329180595 31.755165603160133 10.087802592930213 4 12.157987364049582 15.022283840417316 44.98835162887277 27.83137716666034 5 8.953003222599987 44.04642063476989 34.234268457140686 12.766307685489437 6 24.44700380634771 36.2394043999228 11.918646041303461 27.394945752426032 7 20.005821900470814 35.770199463372656 23.423340758365818 20.800637877790713 8 22.516730212469742 16.304007340726795 19.300416079988608 41.878846366814855 9 13.384952251972889 29.532265515735652 32.8796901599195 24.20309207237196 10-11 28.16452191632151 19.82325189926687 23.793597706641183 28.21862847777043 12-13 38.331885784437034 20.52228829795144 22.35236978783521 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22.101076334616803 27.704814913651788 30.4904819996696 19.70362675206181 40-41 20.514995147997976 31.02216038765193 26.93424941672742 21.52859504762268 42-43 18.49611351429493 30.581789356128542 19.408281955681513 31.513815173895015 44-45 24.128058923246307 20.898782865104724 24.18414149967669 30.789016711972277 46-47 30.639631681960296 28.979626852393174 26.010555146030857 14.37018631961567 48-49 24.90176231963276 24.734444442678928 28.640593891070182 21.72319934661813 50-51 12.146986014497129 28.946749586091403 38.418503700549444 20.48776069886203 52-53 21.346313344285946 33.766194560343706 21.065877739609583 23.82161435576077 54-55 16.250262139439116 23.074466665395686 28.614664556841362 32.06060663832383 56-57 26.573075560680937 20.585277985599166 25.154595288358138 27.68705116536176 58-59 34.641925232546505 20.840486676121518 24.13290368278703 20.384684408544942 60-61 23.113424713207536 25.724641132373815 34.51177809740625 16.650156057012406 62-63 17.637471306364624 27.011811054893208 32.56451600092137 22.786201637820795 64-65 17.034404077896983 36.00145548380536 25.8235559566787 21.14058448161896 66-67 22.649817807942714 24.559139716597194 21.021451466038226 31.769591009421866 68-69 17.46007275824967 25.0108469867627 28.40130513487612 29.127775120111504 70-71 29.413709201925535 24.986531092912415 22.038946199066473 23.560813506095574 72-73 23.36354737417526 18.032443014962944 36.89201128894878 21.711998321913022 74-75 16.591740123283166 35.536952372779815 25.668260544143095 22.203046959793923 76-77 20.370426269825256 23.47351680946624 24.990269081734947 31.165787838973557 78-79 21.904724084251995 24.44403850430473 29.944245004288845 23.70699240715443 80-81 27.671498351932012 22.228664469242684 25.798657718120804 24.3011794607045 82-83 21.4355274048444 20.6858479130823 35.079249335551076 22.799375346522226 84-85 20.583959927620615 26.10376178984877 30.436675187183955 22.87560309534665 86-87 20.068026670521828 28.444902686275043 27.79577383117884 23.691296812024294 88-89 18.946756335524213 27.452270080565338 22.12885688094092 31.472116702969526 90-91 22.79184171555324 19.644616333093932 26.290065273902513 31.27347667745031 92-93 27.52041951374543 26.259335769903068 27.556173526140153 18.664071190211345 94-95 23.04246186314754 22.904201370844834 29.227791312473478 24.825545453534144 96-97 13.894909458402985 25.521829772899174 35.939833853468166 24.643426915229675 98-99 21.220447040651905 29.178253725603614 23.681458904740868 25.919840329003613 100-101 18.458551589476677 21.14657756890857 28.44217145598892 31.952699385625838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2895.0 1 1616.0 2 321.5 3 271.5 4 213.0 5 219.5 6 328.0 7 596.5 8 755.0 9 615.5 10 380.5 11 217.5 12 173.5 13 165.5 14 170.5 15 281.5 16 543.5 17 506.0 18 249.5 19 182.0 20 168.5 21 174.0 22 199.5 23 227.5 24 254.5 25 281.5 26 357.0 27 434.0 28 461.5 29 530.0 30 622.0 31 660.0 32 685.5 33 703.0 34 679.5 35 644.5 36 642.5 37 622.5 38 566.0 39 514.5 40 494.0 41 486.5 42 488.5 43 571.0 44 784.0 45 2172.0 46 10358.5 47 39633.0 48 90658.0 49 89609.5 50 38438.5 51 11469.5 52 4365.5 53 2197.0 54 1304.0 55 820.5 56 557.5 57 458.0 58 401.0 59 332.0 60 279.5 61 261.0 62 217.5 63 186.0 64 171.5 65 146.5 66 115.0 67 85.5 68 75.5 69 73.0 70 65.5 71 59.5 72 48.5 73 33.5 74 31.0 75 28.0 76 19.5 77 16.5 78 15.0 79 8.5 80 6.5 81 3.5 82 2.5 83 3.0 84 0.5 85 1.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32285707961383636 2 0.08727576295143863 3 0.09581360932712284 4 0.09960820771631582 5 0.10909470368929829 6 0.059448708097356744 7 0.06039735769465499 8 0.06134600729195324 9 0.06482438914871347 10-11 0.06197844035681874 12-13 0.05929059983114037 14-15 0.05834195023384212 16-17 0.06039735769465499 18-19 0.06767033794060821 20-21 0.0671960131419591 22-23 0.08095143230278366 24-25 0.08917306214603511 26-27 0.08474603069197664 28-29 0.08316494802981289 30-31 0.07857980830953804 32-33 0.07083250326493569 34-35 0.31321247537463753 36-37 0.48523426901805283 38-39 0.4638896530788423 40-41 0.4491855843207194 42-43 0.4725856077207429 44-45 0.4820721036937253 46-47 0.4778031805058832 48-49 0.4959856311207662 50-51 0.49203292446535696 52-53 0.4901356252707604 54-55 0.48096534583021067 56-57 0.46341532828019316 58-59 0.4457072024639592 60-61 0.4079193268382458 62-63 0.4716369581234446 64-65 0.4955113063221171 66-67 0.5054721270937487 68-69 0.5171721387937604 70-71 0.5140099734694329 72-73 0.5043653692302341 74-75 0.5051559105613159 76-77 0.4808072375639943 78-79 0.4645220861437077 80-81 0.4668937101369534 82-83 0.4757477730450704 84-85 0.47701263917480136 86-87 0.4773288557072341 88-89 0.4823883202261581 90-91 0.49487887325725166 92-93 0.5024680700356376 94-95 0.5108478081451054 96-97 0.49867347164644465 98-99 0.5034167196329358 100-101 0.48333696982345625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 316239.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.928759577408226 #Duplication Level Percentage of deduplicated Percentage of total 1 83.0328983536356 9.074465831222588 2 7.679175949769972 1.67847735415303 3 2.823992361332137 0.925882006963088 4 1.4611845722056653 0.6387573955141523 5 0.943259743641677 0.5154329478653803 6 0.5786869592893724 0.3794598389192984 7 0.3761465235380921 0.28775704451380124 8 0.30959752321981426 0.27068135176243285 9 0.20254043575128033 0.19921641543263163 >10 1.9154538352478225 4.303074573344843 >50 0.2806631752553456 2.1812616407211003 >100 0.25172882729087703 5.815854464503113 >500 0.04340152194670293 3.3923709599385274 >1k 0.05208182633604352 12.186352726893269 >5k 0.03182778276091548 25.526263364101204 >10k+ 0.017360608778681175 32.62469208415154 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34188 10.81081081081081 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23044 7.2868937733802595 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15007 4.745461502218259 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10714 3.387943928484469 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10138 3.205803205803206 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10081 3.187778863454539 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9946 3.145089631576118 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9337 2.952513763324574 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9272 2.9319596887164456 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8212 2.596770164337732 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8146 2.5758998731971703 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6787 2.146161605621065 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6710 2.1218129326237434 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6206 1.9624398002776382 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5409 1.7104152239287376 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5379 1.7009287279557548 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5320 1.6822719525422227 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4130 1.305974278947252 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3517 1.112133544565977 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3450 1.0909470368929828 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3304 1.0447794231578016 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2854 0.9024819835630646 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2315 0.7320412725818132 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2173 0.6871385249763629 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2067 0.6536195725384915 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1994 0.6305357656709007 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1884 0.5957519471032985 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1669 0.5277653926302575 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1536 0.48570859381670195 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1471 0.46515451920857326 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1453 0.45946262162478374 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1283 0.4057058111112165 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1277 0.40380851191662004 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1087 0.34372737075439774 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1074 0.33961655583277206 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 891 0.28174893039757903 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 861 0.2722624344245966 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 833 0.2634083715164796 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 817 0.25834890699755564 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 784 0.24791376142727495 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 752 0.23779483238942697 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 743 0.23494888359753227 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 705 0.2229326553650878 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 679 0.21471102552183632 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 664 0.2099677775353451 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 661 0.2090191279380469 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 659 0.20838669487318137 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 619 0.1957380335758714 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 531 0.16791097872178953 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 529 0.16727854565692402 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 467 0.14767312064609361 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 460 0.14545960491906437 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 453 0.14324608919203513 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 432 0.13660554201094743 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 428 0.13534067588121643 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 421 0.13312716015418719 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 417 0.13186229402445618 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 409 0.1293325617649942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.12711904603796495 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 374 0.118264983129848 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 358 0.11320551861092401 No Hit GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 348 0.11004335328659653 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 346 0.10941092022173105 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5810826621637431E-4 0.0 12-13 0.0 0.0 0.0 3.1621653243274863E-4 0.0 14-15 0.0 0.0 0.0 9.486495972982459E-4 0.0 16-17 0.0 0.0 0.0 0.001897299194596492 0.0 18-19 0.0 0.0 0.0 0.002529732259461989 0.0 20-21 0.0 0.0 0.0 0.003952706655409358 0.0 22-23 0.0 0.0 0.0 0.006482438914871347 0.0 24-25 0.0 0.0 0.0 0.01059325383649708 0.0 26-27 0.0 0.0 0.0 0.02087029114056141 0.0 28-29 0.0 0.0 0.0 0.06529871394736259 0.0 30-31 0.0 0.0 0.0 0.15431366782718134 0.0 32-33 0.0 0.0 0.0 0.3334503334503335 0.0 34-35 0.0 0.0 0.0 0.5601775872046142 0.0 36-37 0.0 0.0 0.0 0.8237440669873102 0.0 38-39 0.0 0.0 0.0 1.2107931026849945 0.0 40-41 0.0 0.0 0.0 1.5739677901840063 0.0 42-43 0.0 0.0 0.0 1.9638627746735855 0.0 44-45 0.0 0.0 0.0 2.444986228770013 0.0 46-47 0.0 0.0 0.0 3.0203422095313988 0.0 48-49 0.0 0.0 0.0 3.415454766806118 0.0 50-51 0.0 0.0 0.0 3.8738106305673874 0.0 52-53 0.0 0.0 0.0 4.30323268161106 0.0 54-55 0.0 0.0 0.0 4.822144011333201 0.0 56-57 0.0 0.0 0.0 5.649208351911055 0.0 58-59 0.0 0.0 0.0 6.181558884261587 0.0 60-61 0.0 0.0 0.0 6.845771710636575 0.0 62-63 0.0 0.0 0.0 7.502711556765611 0.0 64-65 0.0 0.0 0.0 8.128187857917588 0.0 66-67 0.0 0.0 0.0 8.94307786199678 0.0 68-69 0.0 0.0 0.0 9.937736964763992 0.0 70-71 0.0 0.0 0.0 10.823301363841903 0.0 72-73 0.0 0.0 0.0 11.56008588441021 0.0 74-75 0.0 0.0 0.0 12.607236931561257 0.0 76-77 0.0 0.0 0.0 13.378330945898513 0.0 78-79 0.0 0.0 0.0 14.891901378387864 0.0 80-81 0.0 0.0 0.0 16.2325013676365 0.0 82-83 0.0 0.0 0.0 17.159490132463105 0.0 84-85 0.0 0.0 0.0 18.383090004711626 0.0 86-87 0.0 0.0 0.0 19.495223549277604 0.0 88-89 0.0 0.0 0.0 20.804676210081617 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATTAC 40 9.273572E-8 70.9879 6 TCCCATT 40 7.684801E-6 59.175312 4 CCCATTA 40 7.684801E-6 59.175312 5 TGGGTAT 25 0.005091646 56.790314 6 GTACCCA 95 7.2759576E-12 49.94254 1 TACCCAT 95 7.2759576E-12 49.831837 2 ACCCATG 100 1.2732926E-11 47.34025 3 CATTACT 60 1.5271089E-6 47.325264 7 GTAAGCG 180 0.0 43.605797 94-95 ATTACTC 70 4.4105454E-6 40.56451 8 GGGTCGC 25 0.0016029951 38.059002 92-93 AACTCTG 25 0.0016029951 38.059002 74-75 ATCATTA 25 0.0016036218 38.05597 94-95 GCTTTGC 790 0.0 31.830467 1 CTTTGCG 830 0.0 29.658949 2 TTTGCGT 845 0.0 29.132462 3 CGCAGAA 185 3.0013325E-10 28.139343 9 TATCATT 35 0.008328719 27.182837 94-95 TTGCGTT 1020 0.0 24.134243 4 GAGTAAA 50 0.0016886593 23.662632 12-13 >>END_MODULE