FastQCFastQC Report
Fri 27 May 2016
SRR938949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316239
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3610211.416049253887092No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT219986.956131280455605No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT144814.579131606158633No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC109233.4540331837629132No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA108343.4258899123763986No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG104153.293395185287077No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC101743.2171870009707852No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC101483.208965371127533No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT95173.009432739162469No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT85522.7042837853648667No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC82092.5958215147404333No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA73472.3232428637834044No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA68712.1727237943454156No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT57931.831842372382913No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC57611.8217234433450649No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC57051.8040153175288312No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG51241.6202935121854043No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC42311.3379121487229595No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG36171.143755197809252No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG34491.0906308203605501No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC34351.0862037889064917No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT30870.9761604356198951No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG22560.7133844971682809No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT22250.7035817846628658No Hit
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21260.6722763479520236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19220.6077681753357429No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG19120.6046060100114153No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18540.5862654511303159No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA16570.5239707942410645No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA15850.5012032039059067No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT15280.48317886155723994No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA14240.45029234218423403No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13800.43637881475719315No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA12260.38768146876254983No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11260.35605981551927496No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT9170.2899705602408305No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC8970.28364622959217556No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC8480.26815161950297084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC8150.25771647393269015No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7920.2504434936867369No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7850.2482299779597077No Hit
TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7690.2431705134407837No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT7320.231470501740772No Hit
TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA7200.227675903351579No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.2270434702867135No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA7090.2241975214948188No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG7050.2229326553650878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.20838669487318137No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG6320.19984884849749712No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG5640.17834612429207022No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA5490.17360287630557902No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT4640.14672447104879538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.14545960491906437No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC4540.14356230572446788No Hit
CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4530.14324608919203513No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC4390.13881905773797665No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA4270.13502445934878368No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT4250.1343920262839182No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT4120.13028121136229245No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.12711904603796495No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA4000.12648661297309946No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3870.12237579805147374No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT3870.12237579805147374RNA PCR Primer, Index 27 (95% over 23bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3410.1078298375595673No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3340.10561632183253805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGTA250.005041028456.9338652
CCACTGT1050.040.66705394-95
GTAAGCG2150.039.72130694-95
GTTGCTC250.001624504237.9559194-95
AAGCAGG4600.035.06796394-95
GTTGATC3800.031.8380294-95
GCACGCA300.00397212631.62992994-95
GCTTTGC7950.029.9579851
GAGCACG404.5220158E-429.65305992-93
AGCACGC404.5220158E-429.65305994-95
CTCCGAC801.9263098E-929.65305994-95
CTTTGCG8150.029.1072942
TTTGCGT8150.029.1072943
GAGAAAA504.9889553E-528.46693212-13
ATACCAT853.6798156E-927.9087694-95
GCAGAGA8250.027.89184694-95
TTACTCG350.00843639827.11136894-95
CACGAGC350.00843639827.11136894-95
AGTAAGA806.30298E-826.68775494-95
GAGCCCC459.003893E-426.35827494-95
Produced by FastQC (version 0.11.5)