##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938949_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 316239 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.891812837758785 31.0 31.0 34.0 30.0 34.0 2 32.05955305955306 33.0 31.0 34.0 30.0 34.0 3 32.184825401041614 34.0 31.0 34.0 30.0 34.0 4 35.63580077093591 37.0 35.0 37.0 33.0 37.0 5 35.59383883708208 37.0 35.0 37.0 33.0 37.0 6 35.766489901625036 37.0 35.0 37.0 33.0 37.0 7 35.74016487530001 37.0 35.0 37.0 33.0 37.0 8 35.78909622152865 37.0 35.0 37.0 33.0 37.0 9 37.28025322619917 39.0 37.0 39.0 34.0 39.0 10-11 37.1467244710488 39.0 37.0 39.0 33.5 39.0 12-13 37.173052659539145 39.0 37.0 39.0 33.0 39.0 14-15 38.017599031112546 39.5 37.0 41.0 33.0 41.0 16-17 38.38963726801565 40.0 37.5 41.0 33.5 41.0 18-19 38.51612546207141 40.0 38.0 41.0 34.0 41.0 20-21 38.439858777696614 40.0 38.0 41.0 34.0 41.0 22-23 38.41447133339025 40.0 38.0 41.0 34.0 41.0 24-25 38.139517896274654 40.0 37.5 41.0 33.5 41.0 26-27 37.819592143916466 39.5 37.0 41.0 32.5 41.0 28-29 37.81279981279981 39.5 37.0 41.0 33.0 41.0 30-31 37.9672510348186 40.0 37.0 41.0 33.0 41.0 32-33 37.82785804407426 40.0 37.0 41.0 33.0 41.0 34-35 37.51034976710652 39.5 36.5 41.0 32.5 41.0 36-37 37.288546004762225 39.5 36.0 41.0 31.5 41.0 38-39 37.111504273666434 39.0 36.0 40.5 31.0 41.0 40-41 37.400175183958964 39.5 36.5 41.0 32.0 41.0 42-43 37.154438573357496 39.0 36.0 41.0 31.0 41.0 44-45 37.013687432606346 39.0 36.0 41.0 31.0 41.0 46-47 36.79294773889369 39.0 36.0 40.5 31.0 41.0 48-49 36.3809223403818 39.0 35.0 40.0 29.5 41.0 50-51 36.12114887790564 38.5 35.0 40.0 29.0 41.0 52-53 36.17733581247094 39.0 35.0 40.0 28.5 41.0 54-55 36.09675119134579 38.5 35.0 40.0 28.5 41.0 56-57 35.724766711253196 38.5 34.5 40.0 28.0 41.0 58-59 35.415729875189335 38.0 34.0 40.0 28.0 41.0 60-61 34.576987658068745 38.0 33.0 39.5 26.0 41.0 62-63 34.30764548332116 37.0 33.0 40.0 26.0 41.0 64-65 33.896919734757574 36.5 32.5 39.0 26.0 40.5 66-67 33.086059910384236 36.0 32.0 38.5 24.0 39.0 68-69 32.36566014944393 35.5 31.5 37.0 23.0 39.0 70-71 31.35395223233061 34.5 30.5 36.5 21.5 38.0 72-73 30.64137883056802 34.0 29.5 36.0 20.0 37.0 74-75 30.26734052409728 34.0 29.5 35.0 19.0 37.0 76-77 28.432128864561296 31.5 27.5 34.0 16.0 35.0 78-79 29.476451354829734 33.5 29.0 34.5 18.0 36.0 80-81 29.315958815958815 33.5 29.0 35.0 13.0 35.5 82-83 28.84787771274258 33.0 29.0 35.0 9.0 35.0 84-85 28.35651516732598 33.0 28.5 34.0 4.5 35.0 86-87 28.083452705074325 33.0 27.5 34.5 2.0 35.0 88-89 27.288032152897017 32.0 25.5 34.0 2.0 35.0 90-91 26.581593351863624 32.0 25.0 34.0 2.0 35.0 92-93 26.21063657550144 32.0 25.0 34.0 2.0 35.0 94-95 25.530081678730326 31.0 23.5 34.0 2.0 35.0 96-97 24.436125841531247 31.0 18.5 34.0 2.0 35.0 98-99 23.286379921515056 31.0 2.0 34.0 2.0 35.0 100-101 17.78509766347604 23.5 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05798287261955437 1101 2 0.14792640499381804 1101 3 0.20648705613857743 1101 4 0.04056407909224902 1101 5 0.28952141358634265 1101 6 0.2650930289782991 1101 7 0.3352880024475908 1101 8 0.22346317946527705 1101 9 0.6543294341203278 1101 10-11 0.34837826535810734 1101 12-13 0.408672212921914 1101 14-15 0.2649237789717773 1101 16-17 0.4110793942796178 1101 18-19 0.5428793547376856 1101 20-21 0.5212740663571225 1101 22-23 0.32229173354883045 1101 24-25 0.3342534770234238 1101 26-27 0.3225210066079711 1101 28-29 0.2840786096842436 1101 30-31 0.5028153970713376 1101 32-33 0.5495281693591778 1101 34-35 0.47697970960361147 1101 36-37 0.6173406731617774 1101 38-39 0.660690976472182 1101 40-41 0.9079633410661714 1101 42-43 0.6709407194521901 1101 44-45 0.796116509805536 1101 46-47 0.5208054247675875 1101 48-49 0.7869399077840669 1101 50-51 0.790217444031029 1101 52-53 0.6553793862983639 1101 54-55 0.8931816590119865 1101 56-57 1.0681375875801606 1101 58-59 0.8827941791789442 1101 60-61 1.1363563580246847 1101 62-63 1.2503337330428081 1101 64-65 1.110406863951674 1101 66-67 0.9036407239756272 1101 68-69 0.7070259969610575 1101 70-71 1.1579768424017551 1101 72-73 1.2108260112286615 1101 74-75 1.2774147108267293 1101 76-77 0.9588522392934777 1101 78-79 1.0712718305053919 1101 80-81 1.6492762881643266 1101 82-83 1.7130919836195808 1101 84-85 1.7464599289710137 1101 86-87 1.6484774341690134 1101 88-89 1.2693519967652591 1101 90-91 1.1670161105837522 1101 92-93 1.3164298238829382 1101 94-95 1.5036171934532092 1101 96-97 2.1659347919917273 1101 98-99 2.025217895224248 1101 100-101 2.033266499796966 1102 1 0.15832889338080136 1102 2 0.10986412125679124 1102 3 -0.18105619645657356 1102 4 -0.08054318734373567 1102 5 0.12343144818841978 1102 6 0.009037665656499883 1102 7 0.07231222390088021 1102 8 -0.015291145794236627 1102 9 0.3290699185493935 1102 10-11 0.2099699608598371 1102 12-13 -0.1934039116455608 1102 14-15 0.21821097620360774 1102 16-17 0.3418752420304827 1102 18-19 0.11035340318059639 1102 20-21 0.33442285528445836 1102 22-23 0.16139207956959467 1102 24-25 0.4449801206220414 1102 26-27 0.24293623152146182 1102 28-29 0.29099902490915497 1102 30-31 0.3453759507045575 1102 32-33 0.3176942593245826 1102 34-35 0.3022392251745458 1102 36-37 0.1277212959991445 1102 38-39 -0.10574500968699141 1102 40-41 0.2470636870869356 1102 42-43 0.3785531761995884 1102 44-45 0.24767360323113508 1102 46-47 0.5640580199232943 1102 48-49 0.017043714012444866 1102 50-51 0.42343543711061216 1102 52-53 0.3041683136340012 1102 54-55 0.6180951538216704 1102 56-57 0.34149398896425254 1102 58-59 0.40874573627236543 1102 60-61 0.1415466694433718 1102 62-63 0.6430672970566462 1102 64-65 0.9460470023599754 1102 66-67 0.35173760978877766 1102 68-69 0.1257111180683239 1102 70-71 0.7790841088377398 1102 72-73 0.8976772223013256 1102 74-75 0.9383143648059651 1102 76-77 0.514215561092783 1102 78-79 0.8948012422700984 1102 80-81 0.9157122743234964 1102 82-83 0.9829881773912099 1102 84-85 1.471373423780701 1102 86-87 1.4235639393593296 1102 88-89 1.2572412701216606 1102 90-91 1.3850091901685282 1102 92-93 1.3821737685196176 1102 94-95 1.5036171934532092 1102 96-97 0.9617825428567812 1102 98-99 0.7657023242899932 1102 100-101 0.9882838008350276 1103 1 0.3039528526613573 1103 2 0.2847404158822542 1103 3 0.32046133486834094 1103 4 0.38966714131694147 1103 5 0.19869391615492304 1103 6 0.1792964136045896 1103 7 0.18038171148310056 1103 8 0.33206409825078254 1103 9 0.47396168372765857 1103 10-11 0.24399927224309437 1103 12-13 0.27426735682166736 1103 14-15 0.3444317917733599 1103 16-17 0.2925879503219875 1103 18-19 0.451160233842991 1103 20-21 0.33379694742282595 1103 22-23 0.30166039327933447 1103 24-25 0.3954271133119107 1103 26-27 0.309447799007323 1103 28-29 0.3418451814805934 1103 30-31 0.3884100685878096 1103 32-33 0.359039606939632 1103 34-35 0.43218596395840336 1103 36-37 0.34330522079572034 1103 38-39 0.4683010713031308 1103 40-41 0.4134075585042041 1103 42-43 0.5841166704188012 1103 44-45 0.4278169715601976 1103 46-47 0.1915660799632093 1103 48-49 0.4068307872436634 1103 50-51 0.5518292158226501 1103 52-53 0.6639059047153992 1103 54-55 0.9436440041588412 1103 56-57 1.0491950175788674 1103 58-59 0.6798583170203898 1103 60-61 0.6566097916607418 1103 62-63 0.48244516826134287 1103 64-65 0.4271752898524497 1103 66-67 0.625530965717779 1103 68-69 0.23658538144562868 1103 70-71 0.07559319631131345 1103 72-73 -0.10006714748031342 1103 74-75 0.31064687540506597 1103 76-77 0.2775810558552365 1103 78-79 0.3566385770835687 1103 80-81 0.46497594686739774 1103 82-83 0.448805341438117 1103 84-85 0.8350613201398076 1103 86-87 0.9162183791717915 1103 88-89 1.2319333352633173 1103 90-91 0.7649175478099188 1103 92-93 1.1035502934319297 1103 94-95 1.359569813409042 1103 96-97 0.5574401594468803 1103 98-99 1.3559629617712439 1103 100-101 1.786534801697126 1104 1 0.15378220419719568 1104 2 0.1857643066672381 1104 3 0.08837942360554507 1104 4 0.10639069080499297 1104 5 0.22941063970441178 1104 6 0.39090050916772867 1104 7 0.2691189128482918 1104 8 0.2876954975681869 1104 9 0.20775007963209902 1104 10-11 0.3617467125161298 1104 12-13 0.34082025784556436 1104 14-15 0.562861825903056 1104 16-17 0.5980487011752302 1104 18-19 0.5364844659249002 1104 20-21 0.4174146948630977 1104 22-23 0.240226946180357 1104 24-25 0.46027352969416313 1104 26-27 0.43743414713019035 1104 28-29 0.568807638818484 1104 30-31 0.3662257682465082 1104 32-33 0.39999523833894557 1104 34-35 0.5260426192484999 1104 36-37 0.11975573274111895 1104 38-39 0.09970038870926601 1104 40-41 0.3912232581629027 1104 42-43 -0.009739984871302454 1104 44-45 0.02849956541685117 1104 46-47 0.3570950902021224 1104 48-49 0.4068307872436634 1104 50-51 0.5740135161639586 1104 52-53 0.11100488082461624 1104 54-55 0.1876713079131065 1104 56-57 0.21472402781777333 1104 58-59 0.39146241258352177 1104 60-61 0.5456882899542563 1104 62-63 0.5012164993193622 1104 64-65 0.3418510577705334 1104 66-67 0.31836373022289166 1104 68-69 0.6819778729132011 1104 70-71 0.5602348345365691 1104 72-73 0.8640966750452819 1104 74-75 1.2014318583402215 1104 76-77 0.8475469261624013 1104 78-79 0.9658535941484132 1104 80-81 0.9376721926011875 1104 82-83 1.0204776963869229 1104 84-85 0.9459828218462931 1104 86-87 1.3240682085233253 1104 88-89 1.153435041747958 1104 90-91 0.9987059437143557 1104 92-93 1.511400122783467 1104 94-95 1.5728803936138185 1104 96-97 0.8799657567165049 1104 98-99 0.5095465795186875 1104 100-101 1.3838044262705047 1105 1 0.03515013141762324 1105 2 0.22390052865862486 1105 3 0.2532635082106509 1105 4 0.23192860444203234 1105 5 0.061992511016065066 1105 6 0.18032372877346603 1105 7 0.2751914869576666 1105 8 0.017945363446479234 1105 9 0.16396362421186694 1105 10-11 0.40119561500109313 1105 12-13 0.26669376110443466 1105 14-15 0.39365160297566604 1105 16-17 0.2888553718750586 1105 18-19 0.3942698027130547 1105 20-21 0.3713510241518492 1105 22-23 0.20318652703576845 1105 24-25 0.20987116458635313 1105 26-27 0.2570490180879901 1105 28-29 0.45987798707832184 1105 30-31 0.6999662227763963 1105 32-33 0.6982476610905621 1105 34-35 0.6966739614928414 1105 36-37 0.6424879345058159 1105 38-39 0.6542508241767564 1105 40-41 0.9998960811108404 1105 42-43 1.038503876920565 1105 44-45 1.1722723233014918 1105 46-47 0.8513478325628938 1105 48-49 0.8013882997711974 1105 50-51 1.423597453520557 1105 52-53 1.152856559343455 1105 54-55 1.0402173373399748 1105 56-57 0.9264137329783253 1105 58-59 0.9073743830881682 1105 60-61 1.3682481310628418 1105 62-63 1.1575966972487564 1105 64-65 1.2777408839259792 1105 66-67 1.5860306974743708 1105 68-69 0.7610468970779394 1105 70-71 1.4110001843373858 1105 72-73 1.38815297191654 1105 74-75 1.6815704317418927 1105 76-77 1.1995507614723664 1105 78-79 0.9225465524736585 1105 80-81 1.3265338132479378 1105 82-83 1.0355335709174973 1105 84-85 0.7770636715500814 1105 86-87 1.1931939633146094 1105 88-89 1.7520972491058195 1105 90-91 1.2697345144906649 1105 92-93 1.6694925126609874 1105 94-95 1.9442034614052055 1105 96-97 1.4335859012255057 1105 98-99 1.4567254579188145 1105 100-101 1.4112565705055538 1106 1 0.0211437832933008 1106 2 0.15522149238059058 1106 3 0.1904532915649355 1106 4 0.3400703934201559 1106 5 0.15073843692621125 1106 6 0.21775209935054818 1106 7 0.20337034216504435 1106 8 0.22306646776448247 1106 9 0.36436624015034624 1106 10-11 0.3944960005750886 1106 12-13 0.26256108956660995 1106 14-15 0.38904495235682646 1106 16-17 0.05244099699488913 1106 18-19 0.2440397364624971 1106 20-21 0.2981292445468 1106 22-23 0.23939000785293274 1106 24-25 0.5301904880211552 1106 26-27 0.2502588683319047 1106 28-29 0.35704050332142856 1106 30-31 0.20940852514309682 1106 32-33 0.43382058996174067 1106 34-35 0.1592136372698434 1106 36-37 0.25523104849512634 1106 38-39 0.5569839260236762 1106 40-41 0.6204465359177433 1106 42-43 0.5451555608777028 1106 44-45 0.49731546072347754 1106 46-47 0.6428769210318848 1106 48-49 1.0800933250145803 1106 50-51 0.6499357787234601 1106 52-53 0.572940490920054 1106 54-55 0.8586567212736824 1106 56-57 0.5436516598257768 1106 58-59 0.9237932314787685 1106 60-61 1.0116401170884473 1106 62-63 1.122719292736896 1106 64-65 0.859872405132549 1106 66-67 1.013788829677253 1106 68-69 0.9407222480354918 1106 70-71 0.8956677599077558 1106 72-73 0.8972970402547347 1106 74-75 0.9728172630053358 1106 76-77 0.40764888046774317 1106 78-79 0.7817108581209347 1106 80-81 0.16519985504330847 1106 82-83 0.5754330234450258 1106 84-85 1.0525946346329675 1106 86-87 0.9412341396987358 1106 88-89 1.0642630672256637 1106 90-91 0.862181737718835 1106 92-93 1.1338322438243118 1106 94-95 0.7825606179567686 1106 96-97 1.105645633240929 1106 98-99 1.2164659943140173 1106 100-101 1.884532671308655 1107 1 0.04577571396052349 1107 2 -0.05749647775619593 1107 3 0.054283300962445935 1107 4 -0.09009562934271287 1107 5 -0.04310096998618462 1107 6 -0.28426314550691956 1107 7 -0.1419256795536583 1107 8 0.007988677224652463 1107 9 -0.4125263149295293 1107 10-11 -0.02976002949696266 1107 12-13 -0.0012375947813012544 1107 14-15 -0.41972084068900983 1107 16-17 -0.09833690840346065 1107 18-19 -0.33087411965109936 1107 20-21 -0.09749046935108652 1107 22-23 -0.31928181004411726 1107 24-25 -0.25526946812377105 1107 26-27 -0.12279636817222439 1107 28-29 -0.09722492426550389 1107 30-31 0.09434639712805648 1107 32-33 0.16345408688144403 1107 34-35 0.38190341486199486 1107 36-37 0.3151454266928866 1107 38-39 0.09681894742912789 1107 40-41 0.03544079299198444 1107 42-43 0.3291119146419277 1107 44-45 0.23333926776117409 1107 46-47 0.2212365326012673 1107 48-49 0.008291090494374487 1107 50-51 0.08237808842378769 1107 52-53 -0.01841379539090582 1107 54-55 0.1608599164287412 1107 56-57 0.13141400711615603 1107 58-59 0.13741440721229026 1107 60-61 -0.0415550379705536 1107 62-63 -0.1347987191666178 1107 64-65 -0.10843708635747618 1107 66-67 0.302182898801199 1107 68-69 0.056677139964058654 1107 70-71 0.2774731269329216 1107 72-73 -0.4575896674825515 1107 74-75 -0.8457062829902782 1107 76-77 -0.5114750623118312 1107 78-79 -0.012499228545035379 1107 80-81 0.008292681606949515 1107 82-83 -0.13197012994329782 1107 84-85 0.3738069140524125 1107 86-87 0.05599345890099272 1107 88-89 0.18294323846927085 1107 90-91 0.09075540379380698 1107 92-93 0.08365995605152321 1107 94-95 -0.19416487019192274 1107 96-97 0.757227548245627 1107 98-99 0.7092612329064742 1107 100-101 1.1704155156231586 1108 1 0.08521768551627318 1108 2 -0.21620888683230177 1108 3 -0.17171821895938422 1108 4 -0.3230670765254331 1108 5 0.04203636580798076 1108 6 0.04174923099942873 1108 7 -0.026498472952852126 1108 8 -0.06324137380129713 1108 9 -0.1983367589638405 1108 10-11 -0.30152205316431235 1108 12-13 0.03371517907604016 1108 14-15 0.036733398807243134 1108 16-17 -0.07719371005366327 1108 18-19 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-2.2374998024446775 2316 90-91 -2.381250354809268 2316 92-93 -2.9200552717383808 2316 94-95 -2.596366142657441 2316 96-97 -1.777755965431055 2316 98-99 -2.610666320151619 2316 100-101 -2.479986193381624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 5.0 6 28.0 7 53.0 8 75.0 9 156.0 10 289.0 11 380.0 12 581.0 13 749.0 14 995.0 15 1261.0 16 1619.0 17 2069.0 18 2059.0 19 2043.0 20 2308.0 21 2392.0 22 2850.0 23 3091.0 24 3605.0 25 4104.0 26 4773.0 27 5562.0 28 7065.0 29 8466.0 30 10385.0 31 12611.0 32 15601.0 33 19435.0 34 27232.0 35 38132.0 36 56228.0 37 62221.0 38 17738.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.35905412826854 17.92413598255299 14.924240740799528 12.792569148378947 2 14.106103295292485 30.46683046683047 25.70524192145814 29.72182431641891 3 37.80337023580267 20.339679799139258 31.782607458283135 10.074342506774938 4 12.086112086112086 15.555956096496637 44.446447149149854 27.911484668241428 5 8.904657553306201 44.67918251702036 33.77856621099865 12.6375937186748 6 24.086213275402464 37.145323631810115 11.411938438965466 27.35652465382195 7 20.200228308336417 35.494040899446304 22.896290463858033 21.409440328359246 8 23.055347379671705 16.847384414951982 18.79021879021879 41.30704941515752 9 13.203937528261852 29.66016209259453 31.957475200718445 25.17842517842518 10-11 28.410790572952738 20.03057813868625 24.060441628009198 27.498189660351823 12-13 38.571934517880464 20.465850195579925 22.151284313446475 18.810930973093136 14-15 24.99936756493527 26.975885250981857 34.01188345486627 14.012863729216601 16-17 17.243085816199397 28.889524845525784 32.01094758944326 21.85644174883156 18-19 16.896979164360907 38.56285001438795 24.624569547522903 19.91560127372825 20-21 24.377923026571676 25.030277732980437 19.143748873478604 31.448050366969287 22-23 18.4156603075522 25.6797864905973 27.256758337839422 28.64779486401108 24-25 31.635408662435687 26.181622127568073 20.8026208026208 21.380348407375436 26-27 25.636622933920233 18.128851912635696 37.502806421725346 18.731718731718733 28-29 16.742874850982957 38.563396671504776 24.56986646175835 20.123862015753907 30-31 21.132908970746808 25.195026546377896 23.074162263351454 30.597902219523842 32-33 23.094716337959582 26.38747909018179 29.0593506809723 21.45845389088632 34-35 30.111430699339515 22.769053669211953 24.52329260559984 22.596223025848698 36-37 23.267163114623184 20.98570590439924 36.367316666640306 19.379814314337267 38-39 21.817695955422522 28.00101829934522 30.54898035662614 19.632305388606117 40-41 20.51627878295325 31.009615004530012 26.586333443487142 21.8877727690296 42-43 18.77373675406114 30.689784934335563 19.471648710270088 31.06482960133321 44-45 24.485075205029165 20.898507599557924 24.011219486743265 30.605197708669646 46-47 30.678986579643052 29.139629896659457 25.935851705688158 14.245531818009335 48-49 25.216217156337255 24.902840627006036 28.450699326129786 21.43024289052692 50-51 12.039350109727485 29.232413561937527 38.14484660287505 20.583389725459938 52-53 21.314101043830775 33.9645015320691 20.89258440609792 23.828813018002208 54-55 16.452746182475913 23.38310581553825 27.99496583280367 32.16918216918217 56-57 26.7708916357565 20.851160040349228 25.100161586648074 27.277786737246196 58-59 34.692596545318 21.178702715522352 23.808371872406024 20.320328866753627 60-61 22.65468620053947 26.856439948519622 33.99681251719438 16.492061333746523 62-63 17.435004719598904 27.61210430866967 31.971685568778863 22.981205402952558 64-65 16.885753406464136 36.822857540389016 24.97288426085499 21.318504792291858 66-67 22.825222501727364 24.971263322829493 20.421776946465194 31.781737228977953 68-69 17.770989566287042 25.753831413081112 27.37683586072993 29.09834315990191 70-71 29.257144122008988 25.946989460502973 21.21085634599148 23.58501007149656 72-73 23.404292323211244 18.73440657224441 35.92472781661971 21.93657328792464 74-75 16.333374441482547 36.230351095215966 24.41476225260009 23.0215122107014 76-77 20.60145878204834 24.000588703538707 23.847399180562658 31.550553333850296 78-79 21.933298864913837 25.107871922942525 28.79630903309759 24.162520179046044 80-81 27.65476056836849 22.673551765518745 24.777185573546546 24.894502092566213 82-83 21.080733242895406 21.406120054768703 34.27723968264509 23.235907019690806 84-85 19.985390773103212 26.61614572545721 29.550797894626996 23.84766560681258 86-87 19.536835708656557 28.96517564098912 26.415778096386532 25.082210553967787 88-89 18.440990070394914 27.94771752472873 20.9941356098537 32.61715679502265 90-91 22.895924298191733 20.15297174355727 24.800055868493185 32.15104808975781 92-93 26.675394242961808 27.091218983110878 26.503372449318398 19.73001432460892 94-95 22.49186921813263 23.659824213839958 28.042778110686147 25.805528457341264 96-97 12.39078671511104 26.738005116383494 35.003114732844466 25.868093435661006 98-99 20.167974222028278 29.644825590771536 22.289787154652018 27.89741303254817 100-101 17.940544967571995 22.60299963002666 26.308108740541176 33.148346661860174 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2420.0 1 1436.5 2 380.0 3 269.5 4 209.5 5 196.5 6 302.0 7 593.5 8 738.0 9 634.5 10 414.0 11 205.5 12 152.0 13 149.0 14 152.0 15 130.0 16 119.5 17 124.5 18 129.0 19 126.0 20 133.0 21 149.0 22 165.0 23 185.5 24 207.0 25 229.5 26 271.5 27 333.5 28 359.5 29 395.0 30 470.5 31 530.0 32 595.0 33 659.5 34 688.5 35 678.0 36 731.0 37 754.5 38 691.5 39 613.5 40 546.5 41 548.0 42 549.5 43 628.5 44 850.0 45 2092.5 46 8523.5 47 39462.0 48 89848.5 49 83966.0 50 38592.5 51 17920.5 52 8940.5 53 3654.0 54 979.5 55 483.5 56 366.5 57 306.5 58 266.5 59 232.0 60 196.0 61 171.5 62 134.0 63 96.5 64 68.5 65 47.0 66 40.0 67 38.5 68 36.5 69 29.0 70 21.5 71 18.0 72 19.5 73 13.5 74 7.5 75 8.0 76 7.0 77 5.0 78 4.5 79 3.5 80 0.5 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3883139018274153 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.162165324327487E-4 16-17 0.0015810826621637433 18-19 6.324330648654974E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01059325383649708 36-37 0.018814883679748547 38-39 0.00806352157703509 40-41 0.004585139720274856 42-43 0.0031621653243274865 44-45 0.0011067578635146201 46-47 9.48649597298246E-4 48-49 0.001897299194596492 50-51 3.162165324327487E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.74324798649123E-4 60-61 6.324330648654974E-4 62-63 0.0014229743959473689 64-65 6.324330648654974E-4 66-67 0.0017391909283801178 68-69 0.0014229743959473689 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09186090267171348 78-79 0.002371623993245615 80-81 1.5810826621637434E-4 82-83 0.0 84-85 1.5810826621637434E-4 86-87 0.08917306214603511 88-89 0.4340071907639475 90-91 0.38372876210714046 92-93 0.0 94-95 7.905413310818716E-4 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 316239.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.821492605276388 #Duplication Level Percentage of deduplicated Percentage of total 1 79.89389540093917 7.047834074861102 2 9.094167831666487 1.6044826855637666 3 3.2261533498225616 0.8537846375684213 4 1.7528766534035918 0.6185195374384563 5 1.114815213105352 0.4917167079329241 6 0.6237229809656952 0.33013005985978955 7 0.48392300247338427 0.2988246231489475 8 0.3978922464781159 0.2808002808002808 9 0.3297845646485285 0.2618272888543159 >10 2.2367996558769763 4.071287855071639 >50 0.3369537943148009 2.1265561806102347 >100 0.32619994981539235 6.183930508254833 >500 0.057353837330178875 3.734833464563194 >1k 0.06452306699645123 12.661309958607255 >5k 0.032261533498225616 19.883379342838804 >10k+ 0.028676918665089438 39.550782794026034 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36102 11.416049253887092 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 21998 6.956131280455605 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14481 4.579131606158633 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10923 3.4540331837629132 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10834 3.4258899123763986 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10415 3.293395185287077 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10174 3.2171870009707852 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10148 3.208965371127533 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9517 3.009432739162469 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8552 2.7042837853648667 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8209 2.5958215147404333 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7347 2.3232428637834044 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6871 2.1727237943454156 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5793 1.831842372382913 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5761 1.8217234433450649 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5705 1.8040153175288312 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5124 1.6202935121854043 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4231 1.3379121487229595 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3617 1.143755197809252 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3449 1.0906308203605501 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3435 1.0862037889064917 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3087 0.9761604356198951 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2256 0.7133844971682809 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2225 0.7035817846628658 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2126 0.6722763479520236 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1922 0.6077681753357429 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1912 0.6046060100114153 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1854 0.5862654511303159 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1657 0.5239707942410645 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1585 0.5012032039059067 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1528 0.48317886155723994 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1424 0.45029234218423403 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1380 0.43637881475719315 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1226 0.38768146876254983 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1126 0.35605981551927496 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 917 0.2899705602408305 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 897 0.28364622959217556 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 848 0.26815161950297084 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 815 0.25771647393269015 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 792 0.2504434936867369 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 785 0.2482299779597077 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 769 0.2431705134407837 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 732 0.231470501740772 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 720 0.227675903351579 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.2270434702867135 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 709 0.2241975214948188 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 705 0.2229326553650878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.20838669487318137 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 632 0.19984884849749712 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 564 0.17834612429207022 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 549 0.17360287630557902 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 464 0.14672447104879538 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.14545960491906437 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 454 0.14356230572446788 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 453 0.14324608919203513 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 439 0.13881905773797665 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 427 0.13502445934878368 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 425 0.1343920262839182 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 412 0.13028121136229245 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 402 0.12711904603796495 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 400 0.12648661297309946 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 387 0.12237579805147374 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 387 0.12237579805147374 RNA PCR Primer, Index 27 (95% over 23bp) GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 341 0.1078298375595673 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 334 0.10561632183253805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5810826621637431E-4 0.0 12-13 0.0 0.0 0.0 3.1621653243274863E-4 0.0 14-15 0.0 0.0 0.0 9.486495972982459E-4 0.0 16-17 0.0 0.0 0.0 0.001897299194596492 0.0 18-19 0.0 0.0 0.0 0.002529732259461989 0.0 20-21 0.0 0.0 0.0 0.003794598389192984 0.0 22-23 0.0 0.0 0.0 0.006166222382438599 0.0 24-25 0.0 0.0 0.0 0.010277037304064332 0.0 26-27 0.0 0.0 0.0 0.020554074608128664 0.0 28-29 0.0 0.0 0.0 0.06561493047979534 0.0 30-31 0.0 0.0 0.0 0.15510420915826323 0.0 32-33 0.0 0.0 0.0 0.3370868235733101 0.0 34-35 0.0 0.0 0.0 0.5657113765221873 0.0 36-37 0.0 0.0 0.0 0.8335467794927254 0.0 38-39 0.0 0.0 0.0 1.2235998722485208 0.0 40-41 0.0 0.0 0.0 1.5891461837407783 0.0 42-43 0.0 0.0 0.0 1.9807803591587376 0.0 44-45 0.0 0.0 0.0 2.466805169507872 0.0 46-47 0.0 0.0 0.0 3.044374665996288 0.0 48-49 0.0 0.0 0.0 3.4413845224656034 0.0 50-51 0.0 0.0 0.0 3.9029025515512 0.0 52-53 0.0 0.0 0.0 4.3348543348543345 0.0 54-55 0.0 0.0 0.0 4.85835080429675 0.0 56-57 0.0 0.0 0.0 5.68778676886785 0.0 58-59 0.0 0.0 0.0 6.222508925211628 0.0 60-61 0.0 0.0 0.0 6.893362298767704 0.0 62-63 0.0 0.0 0.0 7.556152150746746 0.0 64-65 0.0 0.0 0.0 8.189059540410891 0.0 66-67 0.0 0.0 0.0 9.013119823930634 0.0 68-69 0.0 0.0 0.0 10.018214072268126 0.0 70-71 0.0 0.0 0.0 10.911051451591991 0.0 72-73 0.0 0.0 0.0 11.651946787081922 0.0 74-75 0.0 0.0 0.0 12.701785674758648 0.0 76-77 0.0 0.0 0.0 13.475567529621584 0.0 78-79 0.0 0.0 0.0 14.99593661755824 0.0 80-81 0.0 0.0 0.0 16.341437963059583 0.0 82-83 0.0 0.0 0.0 17.26921726921727 0.0 84-85 0.0 0.0 0.0 18.492659033199573 0.0 86-87 0.0 0.0 0.0 19.606373660427714 0.0 88-89 0.0 0.0 0.0 20.912506047641184 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTGGTA 25 0.0050410284 56.933865 2 CCACTGT 105 0.0 40.667053 94-95 GTAAGCG 215 0.0 39.721306 94-95 GTTGCTC 25 0.0016245042 37.95591 94-95 AAGCAGG 460 0.0 35.067963 94-95 GTTGATC 380 0.0 31.83802 94-95 GCACGCA 30 0.003972126 31.629929 94-95 GCTTTGC 795 0.0 29.957985 1 GAGCACG 40 4.5220158E-4 29.653059 92-93 AGCACGC 40 4.5220158E-4 29.653059 94-95 CTCCGAC 80 1.9263098E-9 29.653059 94-95 CTTTGCG 815 0.0 29.107294 2 TTTGCGT 815 0.0 29.107294 3 GAGAAAA 50 4.9889553E-5 28.466932 12-13 ATACCAT 85 3.6798156E-9 27.90876 94-95 GCAGAGA 825 0.0 27.891846 94-95 TTACTCG 35 0.008436398 27.111368 94-95 CACGAGC 35 0.008436398 27.111368 94-95 AGTAAGA 80 6.30298E-8 26.687754 94-95 GAGCCCC 45 9.003893E-4 26.358274 94-95 >>END_MODULE