##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938948_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 320420 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.628868360277135 33.0 31.0 34.0 30.0 34.0 2 31.54158292241433 33.0 31.0 34.0 28.0 34.0 3 31.64430747144373 33.0 31.0 34.0 28.0 34.0 4 35.012967355346106 37.0 35.0 37.0 32.0 37.0 5 35.10290868235441 37.0 35.0 37.0 32.0 37.0 6 35.33288808438924 37.0 35.0 37.0 33.0 37.0 7 35.3245240621684 37.0 35.0 37.0 33.0 37.0 8 35.369290306472756 37.0 35.0 37.0 33.0 37.0 9 36.7844922289495 39.0 37.0 39.0 33.0 39.0 10-11 36.67173709506273 39.0 37.0 39.0 32.5 39.0 12-13 36.683170526184384 39.0 37.0 39.0 32.0 39.0 14-15 37.48263217027651 40.0 37.0 41.0 32.0 41.0 16-17 37.571203420510585 39.5 37.0 41.0 32.0 41.0 18-19 37.6374882966107 40.0 37.0 41.0 32.0 41.0 20-21 37.415595156357284 40.0 37.0 41.0 32.0 41.0 22-23 37.64028306597591 40.0 37.0 41.0 32.0 41.0 24-25 37.4304740652893 39.5 37.0 41.0 32.0 41.0 26-27 37.19206666250546 39.5 36.5 41.0 31.5 41.0 28-29 37.21992541039885 39.5 36.5 41.0 31.5 41.0 30-31 37.37250639785282 40.0 37.0 41.0 31.5 41.0 32-33 37.09770145434118 40.0 36.0 41.0 31.0 41.0 34-35 36.61701984894826 39.0 35.0 41.0 30.0 41.0 36-37 36.19549029398914 38.5 35.5 40.5 29.5 41.0 38-39 35.92653392422446 38.0 35.0 40.0 30.0 41.0 40-41 36.2246161288309 39.0 35.0 40.0 30.0 41.0 42-43 36.01896885337994 39.0 35.0 40.0 30.0 41.0 44-45 36.0236377254853 39.0 35.0 40.5 30.0 41.0 46-47 35.7600134198864 39.0 35.0 40.5 28.0 41.0 48-49 35.43733537232382 38.5 35.0 40.0 27.5 41.0 50-51 34.096543599026276 37.5 33.5 39.0 24.5 40.5 52-53 34.51543286935896 37.5 33.5 39.5 26.5 40.0 54-55 35.24482866238063 39.0 35.0 40.0 26.5 41.0 56-57 34.90576274889208 38.5 34.0 40.0 24.5 41.0 58-59 34.73434242556644 38.0 34.0 40.0 25.5 41.0 60-61 33.935551775794266 38.0 33.0 40.0 22.0 41.0 62-63 33.772732663379315 37.0 33.0 40.0 22.0 41.0 64-65 33.28680950003121 36.5 32.5 39.5 22.0 40.5 66-67 32.482260782722676 36.0 31.5 39.0 20.5 39.0 68-69 31.864342113476063 36.0 31.0 37.5 19.0 39.0 70-71 30.86275201298296 34.5 30.5 37.0 15.5 38.0 72-73 30.2638505711254 34.0 29.5 36.0 10.5 37.0 74-75 29.871000561762685 34.0 29.0 35.5 7.0 37.0 76-77 29.571610698458272 34.0 29.0 35.0 4.5 36.5 78-79 28.972678047562574 34.0 29.0 35.0 2.0 36.0 80-81 28.326914674489732 34.0 28.0 35.0 2.0 36.0 82-83 27.824708195493415 33.0 27.0 35.0 2.0 35.0 84-85 27.38491355096436 33.0 26.0 35.0 2.0 35.0 86-87 27.364390487485174 33.0 26.0 35.0 2.0 35.0 88-89 26.713393358716683 33.0 25.0 35.0 2.0 35.0 90-91 26.085259971287684 32.0 24.5 35.0 2.0 35.0 92-93 25.463265401660323 32.0 21.0 34.5 2.0 35.0 94-95 24.771449971911863 32.0 18.5 34.0 2.0 35.0 96-97 23.910464390487483 31.0 4.5 34.0 2.0 35.0 98-99 23.256714624555272 31.0 2.0 34.0 2.0 35.0 100-101 20.51731633481056 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11824736939498948 1101 2 0.3003331018171309 1101 3 0.32423343989733056 1101 4 0.39264509164591743 1101 5 0.2509141237593653 1101 6 0.20137697822786294 1101 7 0.19896621773921908 1101 8 0.17155795171682797 1101 9 0.4331521482185181 1101 10-11 0.4451733520634562 1101 12-13 0.4290373089043342 1101 14-15 0.305376476177841 1101 16-17 0.5235228936104974 1101 18-19 0.35986353906830715 1101 20-21 0.6597997061269538 1101 22-23 0.44244916758187713 1101 24-25 0.5120668246332585 1101 26-27 0.37604124173713416 1101 28-29 0.3617513984799601 1101 30-31 0.40079931200840235 1101 32-33 0.6226593936227474 1101 34-35 0.4494983789283822 1101 36-37 0.3990718673287219 1101 38-39 0.11286478586391979 1101 40-41 0.379736501930914 1101 42-43 0.15176502264334601 1101 44-45 0.3276362489684388 1101 46-47 0.02802554188635753 1101 48-49 0.3603255328211077 1101 50-51 0.10740181762667333 1101 52-53 0.02279028752175094 1101 54-55 0.37363655980744426 1101 56-57 0.2582246405277786 1101 58-59 0.45402572516589146 1101 60-61 0.3272059014836657 1101 62-63 0.4135771916155022 1101 64-65 0.7228259391607921 1101 66-67 0.5644016036642867 1101 68-69 0.5433731872441143 1101 70-71 -0.07934983730872958 1101 72-73 -0.057211158238064996 1101 74-75 -0.039401818262376764 1101 76-77 -0.11822881332462387 1101 78-79 -0.06201316988067518 1101 80-81 0.05883280357403109 1101 82-83 -0.45640019235693785 1101 84-85 -0.19089019276612262 1101 86-87 -0.19894644178907583 1101 88-89 0.003297580942867029 1101 90-91 0.27796994825166976 1101 92-93 -0.3438330458275871 1101 94-95 -0.19705112951927006 1101 96-97 -0.45389416212493217 1101 98-99 -0.9240871529679531 1101 100-101 0.22593727510154693 1102 1 0.22836432021928132 1102 2 0.05830595419607221 1102 3 0.36443822126034675 1102 4 0.11280854099071291 1102 5 0.3617825658240861 1102 6 0.24327261488184604 1102 7 0.4454553446003331 1102 8 0.3761749260252216 1102 9 0.30009123045984154 1102 10-11 0.2358008472492159 1102 12-13 0.18029370832193337 1102 14-15 0.410667444192498 1102 16-17 0.30374693193655133 1102 18-19 0.31742776808526685 1102 20-21 0.5060023269526539 1102 22-23 0.5936515535665663 1102 24-25 0.22729861271272256 1102 26-27 0.35009130984102654 1102 28-29 0.5240617643622514 1102 30-31 0.6074359190254484 1102 32-33 0.7170537386511668 1102 34-35 0.7216791125717066 1102 36-37 0.5842322637403541 1102 38-39 0.4535134167408188 1102 40-41 0.6587980772444268 1102 42-43 0.6479723402893001 1102 44-45 0.9235100923764534 1102 46-47 1.0812992256024856 1102 48-49 1.3074041106239278 1102 50-51 1.3063238973786824 1102 52-53 0.942204594036248 1102 54-55 0.8407470759879914 1102 56-57 1.1528632202093334 1102 58-59 0.9283928286368379 1102 60-61 0.9463407471931191 1102 62-63 1.0624663936257406 1102 64-65 0.6936469433409584 1102 66-67 1.285128671439395 1102 68-69 1.080489809290988 1102 70-71 0.6126326905199271 1102 72-73 0.6359338326031363 1102 74-75 0.7782089092750795 1102 76-77 0.48750835261403935 1102 78-79 0.486469757177705 1102 80-81 0.8002963327647699 1102 82-83 0.24094845261041797 1102 84-85 0.186839834005756 1102 86-87 0.4022700346563681 1102 88-89 0.30180399210250997 1102 90-91 0.12824706064657576 1102 92-93 0.5156733183638522 1102 94-95 0.7961737072662309 1102 96-97 0.5158465733971198 1102 98-99 0.25951155070965726 1102 100-101 0.6435315637681214 1103 1 0.18324700381774406 1103 2 0.23266475349513271 1103 3 0.2694699683757982 1103 4 0.14237090871106517 1103 5 0.4523471865655324 1103 6 0.1803928442803624 1103 7 0.346037943994979 1103 8 0.18515742528559542 1103 9 0.22049586394565068 1103 10-11 0.6419818627017477 1103 12-13 0.32111890574575597 1103 14-15 0.12834569457368872 1103 16-17 0.2252666970761723 1103 18-19 0.020114325059388705 1103 20-21 0.339864778876823 1103 22-23 0.23359488667232142 1103 24-25 0.19076098270885922 1103 26-27 0.0812763079066201 1103 28-29 0.19296438378375314 1103 30-31 0.16207737006206457 1103 32-33 0.13572878018413093 1103 34-35 0.5053037456023546 1103 36-37 0.5602000387113364 1103 38-39 0.4782226859882215 1103 40-41 0.3413326121890137 1103 42-43 0.10678074246494162 1103 44-45 0.4003312844294342 1103 46-47 0.5303103996768144 1103 48-49 0.33844573461973937 1103 50-51 0.3206613043562143 1103 52-53 0.39641023341632575 1103 54-55 0.715579237295195 1103 56-57 0.6262878122759687 1103 58-59 0.5302851387799805 1103 60-61 0.3610217967823388 1103 62-63 0.0930756196333391 1103 64-65 -0.2946669320829045 1103 66-67 -0.03200111623043256 1103 68-69 0.19395445506600595 1103 70-71 -0.3479394402918423 1103 72-73 0.01915792854997278 1103 74-75 -0.3447684922405898 1103 76-77 0.10254328581992667 1103 78-79 -0.20096908126474844 1103 80-81 0.19943381256723214 1103 82-83 0.8425506009456889 1103 84-85 0.2938469571936615 1103 86-87 0.3972917683447257 1103 88-89 0.7073079633366675 1103 90-91 0.31449111517423844 1103 92-93 -0.2535171870866364 1103 94-95 -0.4138749944019189 1103 96-97 -0.08890183924710726 1103 98-99 -0.31549243190339027 1103 100-101 0.008930621595659716 1104 1 0.38730471757027374 1104 2 0.1543324442622982 1104 3 0.33132563847889074 1104 4 0.3081727794013034 1104 5 0.4379415062214349 1104 6 0.3293653301136388 1104 7 0.32293878830894585 1104 8 0.2669034028464168 1104 9 0.1941309655591823 1104 10-11 0.10831740354060315 1104 12-13 0.16704867520935096 1104 14-15 0.1292104905501077 1104 16-17 0.35838269352595375 1104 18-19 0.34391783431043166 1104 20-21 0.16659835344271556 1104 22-23 0.3419959244274935 1104 24-25 0.028623116023986483 1104 26-27 0.2805548859999689 1104 28-29 0.2558498438324506 1104 30-31 0.5445220117406819 1104 32-33 0.3793053942803013 1104 34-35 0.2316128874061434 1104 36-37 0.20343756175359573 1104 38-39 0.4336458670719452 1104 40-41 0.5131027130060133 1104 42-43 0.4840650555210857 1104 44-45 0.15033128442943422 1104 46-47 0.23030584811903765 1104 48-49 0.14184119671664774 1104 50-51 0.4271848245310039 1104 52-53 0.3660456536388921 1104 54-55 0.4235285329416385 1104 56-57 0.06511487583846787 1104 58-59 0.1386577292990907 1104 60-61 0.33044670745802307 1104 62-63 0.3671021552151359 1104 64-65 0.3492760824138017 1104 66-67 0.2702280091877398 1104 68-69 0.35532424637708004 1104 70-71 0.039785008400723854 1104 72-73 -0.5213509356087833 1104 74-75 -0.03636062714875976 1104 76-77 -0.1366638328164278 1104 78-79 0.08746313466114941 1104 80-81 -0.13182287253324532 1104 82-83 -0.10507803745580802 1104 84-85 0.18352857572760684 1104 86-87 0.0843892399543833 1104 88-89 -0.10051388869218769 1104 90-91 0.2375185838253877 1104 92-93 0.33355411306584415 1104 94-95 0.612398872829143 1104 96-97 0.822137964125595 1104 98-99 0.5442797626301861 1104 100-101 1.2362467955561982 1105 1 0.1862764847365348 1105 2 0.37335475168648813 1105 3 -0.0206480457316367 1105 4 0.05592896622598431 1105 5 -0.138130295800174 1105 6 0.18663267065041111 1105 7 -0.0895742824474155 1105 8 0.295484790089354 1105 9 0.18116913216775288 1105 10-11 0.020819582000001446 1105 12-13 0.40294315063632524 1105 14-15 0.516322724778064 1105 16-17 0.1907611354917904 1105 18-19 0.2582281340664494 1105 20-21 0.3729855963845097 1105 22-23 0.38613151174014604 1105 24-25 0.2900273509490532 1105 26-27 0.12564463852698537 1105 28-29 0.3593484496184445 1105 30-31 0.4246304990184768 1105 32-33 0.35755347723603137 1105 34-35 0.7025195622057225 1105 36-37 0.7249280636706388 1105 38-39 0.6099485857892617 1105 40-41 0.12284260495442823 1105 42-43 0.08232446646567126 1105 44-45 -0.18848450504425074 1105 46-47 0.31041869231562913 1105 48-49 0.37108047869642746 1105 50-51 0.5943489060925202 1105 52-53 0.2608805264168623 1105 54-55 0.18490009097578763 1105 56-57 0.4034514042455797 1105 58-59 0.5876926717877637 1105 60-61 0.8297604927490596 1105 62-63 0.4315409840753688 1105 64-65 0.39077573386030195 1105 66-67 0.6052750639106463 1105 68-69 0.2613999696256002 1105 70-71 -0.011005772359123256 1105 72-73 0.6638834666219608 1105 74-75 0.5134280622900675 1105 76-77 0.13958660287545044 1105 78-79 0.26580271291141244 1105 80-81 -0.11710737933004012 1105 82-83 0.02784066243962613 1105 84-85 0.3602016325418269 1105 86-87 0.42152761569506225 1105 88-89 0.314362810506136 1105 90-91 0.35465434541479013 1105 92-93 0.5182656850421381 1105 94-95 0.4957096954154103 1105 96-97 0.5668225232054169 1105 98-99 0.43509537782712115 1105 100-101 0.8181241918615321 1106 1 0.1685133268417971 1106 2 0.06447317273911679 1106 3 0.17195063847889003 1106 4 -0.31413682324770065 1106 5 0.043777598936671325 1106 6 0.10587609170303836 1106 7 0.049077033342058485 1106 8 -0.21685073622643358 1106 9 -0.03856770993751013 1106 10-11 -0.13978897063157802 1106 12-13 0.007383940110010201 1106 14-15 -0.03754240680088117 1106 16-17 0.15178087233390158 1106 18-19 0.3768958972243368 1106 20-21 0.26870928059503996 1106 22-23 0.2443551959506749 1106 24-25 0.26362932463326416 1106 26-27 0.010759770105927657 1106 28-29 0.20951292330264693 1106 30-31 0.3788245779658439 1106 32-33 0.0948890035518204 1106 34-35 0.0031774569425593313 1106 36-37 0.13093135314432658 1106 38-39 0.12697161210505925 1106 40-41 0.37950378916495 1106 42-43 0.2773080190972408 1106 44-45 0.3175187844294314 1106 46-47 0.16995816599983726 1106 48-49 0.13933705764380022 1106 50-51 0.13612522188198994 1106 52-53 0.19344631589054728 1106 54-55 0.11606785413368215 1106 56-57 -0.09210780628072968 1106 58-59 0.03728148757723915 1106 60-61 0.1886420716964352 1106 62-63 0.4430541419701086 1106 64-65 0.06659810228135399 1106 66-67 0.6614428270685337 1106 68-69 0.7303966801519124 1106 70-71 0.47271133290403355 1106 72-73 0.1420907034640635 1106 74-75 0.048707667553223644 1106 76-77 0.4909023923491347 1106 78-79 0.17320402870088358 1106 80-81 0.2049320943541737 1106 82-83 -0.2967481533498493 1106 84-85 -0.4527917885108046 1106 86-87 -0.4049526474628351 1106 88-89 -0.3970681105464955 1106 90-91 -0.5219081545852085 1106 92-93 0.1034630534631944 1106 94-95 -0.6790435940582746 1106 96-97 -0.720184055741953 1106 98-99 -0.14705922743603494 1106 100-101 -0.2027639660332028 1107 1 -0.025899856437945346 1107 2 -0.13627039639271388 1107 3 0.054798307289178894 1107 4 0.4076276462056754 1107 5 0.1419086278755728 1107 6 0.09059273157442504 1107 7 0.11934431630025699 1107 8 0.04535786505974215 1107 9 0.09531492671844433 1107 10-11 0.205754638693179 1107 12-13 0.13474527451515428 1107 14-15 0.274128614099439 1107 16-17 0.04871616172297166 1107 18-19 0.03773291490922759 1107 20-21 0.275401402781533 1107 22-23 0.18002641781562545 1107 24-25 0.283771004697563 1107 26-27 0.35959919936637874 1107 28-29 0.19422956317403361 1107 30-31 0.13049760851247072 1107 32-33 -0.3875969289240615 1107 34-35 -0.370636851739107 1107 36-37 -0.23338235264345997 1107 38-39 -0.25949844416503964 1107 40-41 -0.4607745468479081 1107 42-43 -0.111051104696962 1107 44-45 -0.1800545676605978 1107 46-47 -0.27501771824453414 1107 48-49 -0.15365932499285861 1107 50-51 -0.32464648229807125 1107 52-53 -0.47114371626378926 1107 54-55 -0.6518531667666352 1107 56-57 -0.4836170506537272 1107 58-59 -0.36124644650636384 1107 60-61 -0.28109265499167435 1107 62-63 -0.4652055043321397 1107 64-65 -0.10644249257395444 1107 66-67 -0.04940423884786327 1107 68-69 -0.17679282949438857 1107 70-71 -0.00919180214419768 1107 72-73 -0.22906584798288065 1107 74-75 -0.10835121508343093 1107 76-77 0.11224483607903935 1107 78-79 -0.08868302917693072 1107 80-81 -0.07995536545289994 1107 82-83 0.02943251385272916 1107 84-85 -0.08113784960405113 1107 86-87 -0.10546510726990732 1107 88-89 -0.1799308517040501 1107 90-91 -0.2142765227524137 1107 92-93 -0.1320875896236231 1107 94-95 -0.3759638111000747 1107 96-97 -0.5002785091181607 1107 98-99 0.04391243655109989 1107 100-101 0.635791098275476 1108 1 0.06621581905363527 1108 2 0.11238983940578606 1108 3 0.2191761057686108 1108 4 0.16649012379279782 1108 5 0.27066622822015773 1108 6 0.29132196397718246 1108 7 0.15366231371589123 1108 8 0.32778516720036066 1108 9 0.5167671810282215 1108 10-11 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-2.0585569821049106 2316 58-59 -2.120393649785399 2316 60-61 -2.2051766096222494 2316 62-63 -2.5236354184694534 2316 64-65 -2.908710963652709 2316 66-67 -3.358938354250146 2316 68-69 -3.5131987868810484 2316 70-71 -3.2037653154476153 2316 72-73 -3.152809708623849 2316 74-75 -3.259688623655567 2316 76-77 -3.6287404647937187 2316 78-79 -3.429920971299115 2316 80-81 -3.1066063671842876 2316 82-83 -3.1486884006025946 2316 84-85 -3.5432107445547594 2316 86-87 -3.8793688562540467 2316 88-89 -3.4431017643926474 2316 90-91 -3.798504995244553 2316 92-93 -4.576550682800544 2316 94-95 -5.007597852300034 2316 96-97 -5.68361125354415 2316 98-99 -6.194603870293182 2316 100-101 -6.44476259240683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1896.0 3 359.0 4 301.0 5 313.0 6 283.0 7 331.0 8 436.0 9 535.0 10 688.0 11 921.0 12 1283.0 13 1832.0 14 1725.0 15 1679.0 16 1968.0 17 2299.0 18 2166.0 19 2038.0 20 2340.0 21 2612.0 22 2952.0 23 3361.0 24 3833.0 25 4491.0 26 5017.0 27 5839.0 28 7005.0 29 8121.0 30 9663.0 31 11897.0 32 14998.0 33 18693.0 34 25225.0 35 36039.0 36 49923.0 37 59720.0 38 27386.0 39 252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.46970834897985 17.29471773688822 14.206721742395795 13.028852171736139 2 13.823045280343326 29.499533047435513 26.04033595597215 30.63708571624901 3 37.8318777565376 20.3224758549171 31.71783050339218 10.127815885153117 4 12.166206405338198 14.850612918140127 45.105089532282854 27.87809114423882 5 9.04714449501876 44.01204619791878 34.13485118759392 12.805958119468544 6 24.392369244607686 36.282317960634394 11.90681433452642 27.418498460231493 7 19.977698441702042 35.70685632361623 23.5359526732197 20.779492561462032 8 22.541985901107857 16.269321947608592 19.2124734903972 41.97621866088635 9 13.468247993927903 29.37470521906713 32.85491624317123 24.30213054383373 10-11 28.135741447835898 19.821499683759285 23.86126012165507 28.18149874674975 12-13 38.47408048301679 20.39999437771451 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19.699610745983907 38-39 22.096100420562873 27.66726433341334 30.57888965754166 19.657745588482133 40-41 20.500287746995905 31.039354694039485 26.971850973713877 21.488506585250732 42-43 18.455295774736253 30.512151280064497 19.408011343495215 31.62454160170403 44-45 24.03186028415081 20.972281490034643 24.154230885084594 30.841627340729953 46-47 30.60904675852722 29.02501694149537 25.92817433426198 14.437761965715435 48-49 24.901624220211126 24.690437936964187 28.66485082026886 21.743087022555823 50-51 12.161746617466175 28.903563270326583 38.37716821045761 20.557521901749627 52-53 21.247326943623808 33.788060165145076 21.17468570505147 23.789927186179654 54-55 16.335466381353843 23.02421132392784 28.547806893002985 32.092515401715325 56-57 26.539369368662907 20.57511194059111 25.218195933282622 27.66732275746336 58-59 34.65853666186771 20.797380331787004 24.070867796461314 20.47321520988398 60-61 23.184709581748812 25.65233203018456 34.575870533684885 16.587087854381746 62-63 17.6717045356507 27.003866636340106 32.62437157355629 22.700057254452908 64-65 17.072788012973707 35.932834240038666 25.76711664566625 21.22726110132138 66-67 22.63516111364817 24.58550487193611 21.007939160824897 31.771394853590827 68-69 17.4501489332986 24.926004161971633 28.36120012931616 29.262646775413607 70-71 29.391087104580105 24.93887645668624 22.09345389605702 23.57658254267663 72-73 23.35803763234166 17.94919272962366 36.94833124369381 21.74443839434087 74-75 16.536768686401118 35.43523799810135 25.717132568119943 22.31086074737759 76-77 20.34242395241898 23.416959916661046 25.109154890907863 31.131461240012108 78-79 21.91085519969508 24.36432137552212 30.044216347971208 23.68060707681159 80-81 27.618370944771726 22.227764079978417 25.924601456776646 24.229263518473207 82-83 21.43920486543044 20.676824418763342 35.12911054045871 22.754860175347503 84-85 20.608472746896926 26.025364274150025 30.588047038742953 22.778115940210093 86-87 20.118934452716417 28.439797080443107 27.812183330352912 23.62908513648756 88-89 18.976190924437248 27.3724356922768 22.21824776750695 31.433125615779005 90-91 22.735553153130535 19.669427712024902 26.358856396875037 31.236162737969526 92-93 27.523855703007676 26.253434577862723 27.578149886468665 18.644559832660935 94-95 23.053976782357353 22.882151162608235 29.166418212574186 24.89745384246023 96-97 13.881631493799437 25.34407898556524 36.079060885229595 24.69522863540573 98-99 21.21194527306194 29.198161541442992 23.700674593149245 25.889218592345824 100-101 18.367987298339965 21.20640668217767 28.534492631750695 31.89111338773166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2781.0 1 1577.5 2 371.5 3 298.0 4 204.0 5 210.0 6 340.0 7 599.0 8 754.0 9 660.0 10 411.0 11 217.0 12 182.0 13 176.0 14 165.5 15 246.5 16 513.5 17 534.5 18 309.5 19 218.5 20 183.5 21 191.0 22 218.0 23 235.0 24 271.5 25 301.5 26 344.0 27 440.0 28 504.0 29 563.0 30 619.0 31 634.0 32 678.0 33 692.0 34 686.5 35 671.5 36 624.0 37 592.5 38 567.5 39 525.0 40 472.5 41 462.0 42 486.5 43 570.5 44 787.0 45 2179.5 46 10290.0 47 40275.5 48 92622.0 49 90981.0 50 38585.0 51 11429.5 52 4308.5 53 2204.5 54 1264.0 55 800.0 56 601.5 57 483.0 58 400.5 59 352.0 60 306.0 61 264.5 62 215.5 63 170.0 64 149.0 65 133.5 66 112.0 67 96.5 68 85.0 69 67.5 70 64.5 71 56.5 72 43.5 73 38.0 74 33.0 75 30.5 76 23.5 77 17.5 78 12.0 79 7.5 80 6.0 81 5.0 82 2.5 83 1.5 84 1.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2683977279820236 2 0.08083140877598152 3 0.08488858373384932 4 0.09737219898882717 5 0.09955683165844828 6 0.07552587229261594 7 0.08207977030147931 8 0.07895886648773486 9 0.0833281318269771 10-11 0.07880282129704763 12-13 0.08285999625491543 14-15 0.08800948754759379 16-17 0.0792709568691093 18-19 0.08972598464515323 20-21 0.09097434617065102 22-23 0.10548654890456277 24-25 0.11625366706198115 26-27 0.11906248049435117 28-29 0.11297671805754947 30-31 0.10657886523937332 32-33 0.09940078646776107 34-35 0.35406653766930907 36-37 0.5410086761126022 38-39 0.5244678858997566 40-41 0.48795331127894637 42-43 0.5140128581237126 44-45 0.5355470944385493 46-47 0.5230634791835715 48-49 0.5442856251170339 50-51 0.538043817489545 52-53 0.5400724049684789 54-55 0.5322701454341178 56-57 0.5032457399662942 58-59 0.4996567005804881 60-61 0.4522189626115723 62-63 0.5204107109418887 64-65 0.5541164721303289 66-67 0.5647275450970601 68-69 0.5683165844828663 70-71 0.5626989576181263 72-73 0.5569252855626989 74-75 0.5531802009862056 76-77 0.5372635915361089 78-79 0.5138568129330254 80-81 0.5099556831658448 82-83 0.5247799762811309 84-85 0.5318020098620561 86-87 0.5221272080394482 88-89 0.5361712752012984 90-91 0.5494351164097123 92-93 0.5572373759440734 94-95 0.5564571499906373 96-97 0.5553648336558267 98-99 0.5566131951813245 100-101 0.5372635915361089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 320420.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.112914299981275 #Duplication Level Percentage of deduplicated Percentage of total 1 82.92799370927881 9.215716871606016 2 7.796000898674456 1.7327257973909242 3 2.6623230734666365 0.8875850446289244 4 1.5108964277690407 0.6716185007178079 5 0.836890586385082 0.4650146682479246 6 0.6178386879352954 0.41195930341426873 7 0.4465288699168726 0.3473565944697585 8 0.2808357672433161 0.24967230509955682 9 0.3089193439676477 0.3089694775607016 >10 1.951808582341047 4.318706697459584 >50 0.26117726353628395 2.0900692840646653 >100 0.26117726353628395 6.1122901192185255 >500 0.039317007414064256 3.2482366893452346 >1k 0.050550438103796905 12.140003745084577 >5k 0.03089193439676477 25.28930778353411 >10k+ 0.016850146034598966 32.51076711815742 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34449 10.751201547968293 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 23325 7.279508145558953 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14920 4.656388490106735 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10783 3.3652705823606515 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10351 3.2304475376068913 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10343 3.227950814555895 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9969 3.1112290119218526 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9517 2.970164159540603 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9183 2.865925972161538 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8329 2.599400786467761 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8105 2.529492541039885 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6882 2.1478060046189373 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6692 2.088508832157793 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6153 1.9202921165969666 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5437 1.696835403532863 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5406 1.6871606017102554 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5359 1.6724923537856564 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4165 1.2998564384245677 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3580 1.1172835653205169 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3497 1.091380063666438 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3395 1.0595468447662444 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2872 0.8963235753074091 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2397 0.7480806441545471 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2289 0.714374882966107 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1979 0.6176268647400287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1945 0.6070157917732976 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1815 0.5664440421946195 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1659 0.5177579427002059 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1578 0.49247862180887586 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1435 0.44784969727233004 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1374 0.42881218400848886 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1361 0.424755009050621 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1325 0.413519755321141 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1139 0.35547094438549404 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1094 0.34142687722364395 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 943 0.2943012296361026 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 894 0.27900880094875474 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 841 0.2624680107359091 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 819 0.2556020223456713 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 810 0.2527932089133013 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 760 0.23718868984457897 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 735 0.22938643031021783 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 700 0.2184632669621122 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 698 0.21783908619936332 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 686 0.21409400162286998 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 679 0.21190936895324886 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 659 0.20566756132575992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 0.1984894825541477 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 548 0.17102552899319642 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 488 0.15230010611072967 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 480 0.14980338305973412 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 473 0.14761875039011296 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 469 0.1463703888646152 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 420 0.13107796017726733 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 420 0.13107796017726733 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 416 0.12982959865176955 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 415 0.1295175082703951 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 414 0.12920541788902065 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 394 0.12296361026153174 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 389 0.12140315835465951 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 360 0.11235253729480058 No Hit GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 360 0.11235253729480058 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 347 0.10829536233693277 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.12090381374446E-4 0.0 16-17 0.0 0.0 0.0 6.24180762748892E-4 0.0 18-19 0.0 0.0 0.0 0.0018725422882466761 0.0 20-21 0.0 0.0 0.0 0.0037450845764933523 0.0 22-23 0.0 0.0 0.0 0.007334123962299482 0.0 24-25 0.0 0.0 0.0 0.011547344110854504 0.0 26-27 0.0 0.0 0.0 0.019817739217277322 0.0 28-29 0.0 0.0 0.0 0.06319830222832531 0.0 30-31 0.0 0.0 0.0 0.15682541664065913 0.0 32-33 0.0 0.0 0.0 0.3528181761438113 0.0 34-35 0.0 0.0 0.0 0.5976530803320641 0.0 36-37 0.0 0.0 0.0 0.8796267399038762 0.0 38-39 0.0 0.0 0.0 1.2795705636352288 0.0 40-41 0.0 0.0 0.0 1.647056987703639 0.0 42-43 0.0 0.0 0.0 2.0674427314150177 0.0 44-45 0.0 0.0 0.0 2.533081580425691 0.0 46-47 0.0 0.0 0.0 3.0992135322389363 0.0 48-49 0.0 0.0 0.0 3.5292740777729232 0.0 50-51 0.0 0.0 0.0 3.9850820797703017 0.0 52-53 0.0 0.0 0.0 4.408744772486112 0.0 54-55 0.0 0.0 0.0 4.9324324324324325 0.0 56-57 0.0 0.0 0.0 5.731227763560327 0.0 58-59 0.0 0.0 0.0 6.276293614630797 0.0 60-61 0.0 0.0 0.0 6.937457087572561 0.0 62-63 0.0 0.0 0.0 7.573653330004369 0.0 64-65 0.0 0.0 0.0 8.208913301292053 0.0 66-67 0.0 0.0 0.0 9.060451906872231 0.0 68-69 0.0 0.0 0.0 10.053991635977779 0.0 70-71 0.0 0.0 0.0 10.9782473004182 0.0 72-73 0.0 0.0 0.0 11.714624555271207 0.0 74-75 0.0 0.0 0.0 12.786967105673803 0.0 76-77 0.0 0.0 0.0 13.522252044191998 0.0 78-79 0.0 0.0 0.0 15.040883839960053 0.0 80-81 0.0 0.0 0.0 16.416890331439987 0.0 82-83 0.0 0.0 0.0 17.357374695711876 0.0 84-85 0.0 0.0 0.0 18.554241308282876 0.0 86-87 0.0 0.0 0.0 19.66278634292491 0.0 88-89 0.0 0.0 0.0 20.971069221646587 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 70 7.997551E-8 47.423626 1 ACCCATG 85 7.39783E-9 44.564266 3 AGTAATG 45 9.773463E-4 42.081898 7 GTAATGG 50 0.0016406865 37.873707 8 GTAAGCG 145 0.0 36.078175 94-95 GCTTTGC 985 0.0 35.627907 1 TACCCAT 95 8.8918387E-7 34.88913 2 GTGGAGA 35 2.0377521E-4 33.96971 92-93 CTTTGCG 1050 0.0 33.821095 2 TTTGCGT 1050 0.0 33.821095 3 TGGAGAT 30 0.003926283 31.705063 92-93 CATTAAA 30 0.003926283 31.705063 94-95 CCGCATC 30 0.003926283 31.705063 92-93 GAGTAAT 60 0.0040115723 31.561422 6 TTGCGTT 1150 0.0 30.880127 4 ACAGTAA 35 0.008339375 27.17577 92-93 TGTGGAG 35 0.008339375 27.17577 90-91 CTGTGGA 35 0.008339375 27.17577 90-91 AGTACTT 480 0.0 25.645658 12-13 TACTTTT 525 0.0 23.90024 14-15 >>END_MODULE