##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 320420 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.885821733974158 31.0 31.0 34.0 30.0 34.0 2 32.050327694900446 33.0 31.0 34.0 30.0 34.0 3 32.17201797640597 34.0 31.0 34.0 30.0 34.0 4 35.65514012858124 37.0 35.0 37.0 33.0 37.0 5 35.59254103988515 37.0 35.0 37.0 33.0 37.0 6 35.76180325822358 37.0 35.0 37.0 33.0 37.0 7 35.7421665314275 37.0 35.0 37.0 33.0 37.0 8 35.79058111229012 37.0 35.0 37.0 33.0 37.0 9 37.291255227513886 39.0 37.0 39.0 34.0 39.0 10-11 37.17328194245053 39.0 37.0 39.0 33.5 39.0 12-13 37.21067661194682 39.0 37.0 39.0 33.0 39.0 14-15 37.98710754634543 40.0 37.0 41.0 33.0 41.0 16-17 38.17214281255852 39.5 37.5 41.0 33.5 41.0 18-19 38.456925285562704 40.0 38.0 41.0 34.0 41.0 20-21 38.41456837900256 40.0 38.0 41.0 34.0 41.0 22-23 38.396744897322264 40.0 38.0 41.0 34.0 41.0 24-25 38.16056269895762 40.0 37.5 41.0 33.5 41.0 26-27 37.836793895512145 39.5 37.0 41.0 33.0 41.0 28-29 37.80833749453842 39.5 37.0 41.0 32.5 41.0 30-31 37.98243867424006 40.0 37.0 41.0 33.0 41.0 32-33 37.77497191186568 40.0 37.0 41.0 33.0 41.0 34-35 37.55499188565008 39.5 36.5 41.0 32.5 41.0 36-37 37.38743368079396 39.5 36.0 41.0 32.0 41.0 38-39 37.21432494850509 39.0 36.0 40.5 31.0 41.0 40-41 37.50914580862618 40.0 37.0 41.0 33.0 41.0 42-43 37.27258598090007 40.0 36.0 41.0 31.5 41.0 44-45 37.12278415829224 39.0 36.0 41.0 31.0 41.0 46-47 36.945839835216276 39.0 36.0 41.0 31.0 41.0 48-49 36.59890768366519 39.0 35.5 40.5 30.0 41.0 50-51 36.320832032956744 39.0 35.0 40.0 30.0 41.0 52-53 36.422873104050936 39.0 35.0 40.0 29.5 41.0 54-55 36.385124211971785 39.0 35.5 40.5 30.0 41.0 56-57 35.81642219586792 38.5 34.5 40.0 28.0 41.0 58-59 35.53148367767305 38.0 35.0 40.0 28.0 41.0 60-61 34.57477685537732 38.0 33.0 39.5 26.0 41.0 62-63 34.468388365270584 37.0 33.5 39.5 26.5 40.5 64-65 34.259905748704824 37.0 33.5 39.0 26.0 40.5 66-67 33.44294987828475 36.0 32.5 38.5 25.5 39.0 68-69 32.79538574371138 36.0 32.0 37.0 25.0 39.0 70-71 31.842857811622245 34.5 31.5 36.5 23.0 38.0 72-73 31.155893826852257 34.0 30.5 36.0 21.5 37.0 74-75 30.859139566818552 34.0 30.5 35.0 20.5 37.0 76-77 28.928492291367583 31.5 27.5 34.0 20.0 35.0 78-79 29.997194307471446 34.0 29.5 35.0 20.0 36.0 80-81 29.855939079957555 34.0 29.5 35.0 18.5 35.5 82-83 29.510440983708882 33.5 29.5 35.0 16.0 35.0 84-85 29.117399038761626 33.0 29.0 35.0 12.5 35.0 86-87 28.972147493914235 34.0 29.0 35.0 7.0 35.0 88-89 28.317054178890206 33.0 28.0 35.0 2.0 35.0 90-91 27.76108232944261 33.0 27.0 34.0 2.0 35.0 92-93 27.49294051557331 33.0 26.0 34.0 2.0 35.0 94-95 26.95873540977467 32.0 25.0 34.0 2.0 35.0 96-97 26.215601398164907 32.0 25.0 34.0 2.0 35.0 98-99 25.430140128581236 32.0 22.0 34.0 2.0 35.0 100-101 21.034233193932963 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12015589842707541 1101 2 -0.08385225073194391 1101 3 0.03204828453672093 1101 4 0.060803408163060624 1101 5 0.20185095716501422 1101 6 0.16020764812489574 1101 7 0.15146241851203257 1101 8 0.26028914212281506 1101 9 0.30428383770199474 1101 10-11 0.19094755574784728 1101 12-13 0.11400580952717831 1101 14-15 0.15440670963117498 1101 16-17 0.25140741737516237 1101 18-19 0.2778311860708911 1101 20-21 0.44783652584904843 1101 22-23 0.5463479031412319 1101 24-25 0.6411343813415158 1101 26-27 0.5714848121113931 1101 28-29 0.33051793073884284 1101 30-31 0.3945789089713898 1101 32-33 0.15458284379599263 1101 34-35 0.4129636638284353 1101 36-37 0.26503287353454397 1101 38-39 0.49118359888135643 1101 40-41 0.40463948109051273 1101 42-43 0.16713224066428722 1101 44-45 0.11385344426237509 1101 46-47 -0.05397038766984963 1101 48-49 -0.07124914579740249 1101 50-51 -0.38149872614371816 1101 52-53 -0.015302436358915372 1101 54-55 0.29467142483326114 1101 56-57 -0.0766909362773518 1101 58-59 -0.11072923549939162 1101 60-61 -0.1295751279356594 1101 62-63 0.37005447091180343 1101 64-65 0.13082612352281586 1101 66-67 0.5163342894635505 1101 68-69 0.6465254395233373 1101 70-71 0.5999570873679119 1101 72-73 0.6247687546741254 1101 74-75 0.05988412261687159 1101 76-77 -0.10747967884721987 1101 78-79 -0.10337247168321895 1101 80-81 0.27626130864272724 1101 82-83 0.6598514245184219 1101 84-85 0.2226239351772783 1101 86-87 0.26803534818887087 1101 88-89 0.21171060182951607 1101 90-91 0.3337238451925941 1101 92-93 0.498332888768271 1101 94-95 0.5793270794620788 1101 96-97 0.4371439386275995 1101 98-99 -0.12864426693166564 1101 100-101 0.25935599864226333 1102 1 0.02779393953262499 1102 2 0.05709932505124726 1102 3 0.007084684893683857 1102 4 0.14771806693907052 1102 5 0.14877688830962654 1102 6 0.0721892365895016 1102 7 -0.018398085926463636 1102 8 0.03113128199412074 1102 9 0.3421871771411986 1102 10-11 0.11969679251456 1102 12-13 0.2109012113401505 1102 14-15 0.35754418555996637 1102 16-17 0.2685273271962174 1102 18-19 0.31377360305459234 1102 20-21 0.23880454050313205 1102 22-23 0.23222456464139185 1102 24-25 0.27882166704908684 1102 26-27 0.2935387316078959 1102 28-29 0.15415233025037622 1102 30-31 0.33268656009158093 1102 32-33 0.2777921810746591 1102 34-35 0.39665395550097315 1102 36-37 0.3079500685934917 1102 38-39 0.48220093014808896 1102 40-41 0.34560045051421184 1102 42-43 0.29577814738543395 1102 44-45 0.3566555672251255 1102 46-47 0.8075086410277166 1102 48-49 0.6761910871648737 1102 50-51 0.7347358330597018 1102 52-53 0.6847703643550034 1102 54-55 0.7507796395718884 1102 56-57 0.46815195508519736 1102 58-59 0.3696145717431065 1102 60-61 0.7958041402997438 1102 62-63 1.1478009365545674 1102 64-65 0.6923075006305126 1102 66-67 0.6725857572198777 1102 68-69 1.0859834264661785 1102 70-71 1.0920429452421345 1102 72-73 1.2158635834750235 1102 74-75 0.9797094009034737 1102 76-77 0.6669555285173949 1102 78-79 0.6388073225960547 1102 80-81 0.7806176094270967 1102 82-83 0.9371869183890666 1102 84-85 1.1859535110544108 1102 86-87 0.9856742566478438 1102 88-89 1.239081321852062 1102 90-91 1.3416027611664845 1102 92-93 1.5279111143095783 1102 94-95 1.6708915902882495 1102 96-97 0.9476413318923598 1102 98-99 1.33355807208482 1102 100-101 1.2531327118074493 1103 1 0.3063265173073688 1103 2 0.18506637177299012 1103 3 -0.10899141715223948 1103 4 0.1376477453715168 1103 5 0.07397203634904059 1103 6 0.031816919163865975 1103 7 0.21251381867288188 1103 8 0.2137511586469074 1103 9 0.19208818075968281 1103 10-11 0.18791326460213753 1103 12-13 0.08468651436010788 1103 14-15 0.31198878172300937 1103 16-17 6.397051575746104E-4 1103 18-19 0.2465926103598406 1103 20-21 0.34746729283011035 1103 22-23 0.3401988938555718 1103 24-25 0.06248043676302473 1103 26-27 -0.013731244041267132 1103 28-29 -0.013015007771059572 1103 30-31 -0.009840919938461923 1103 32-33 0.3309373074941746 1103 34-35 -0.01037478758634336 1103 36-37 -0.15263913055991196 1103 38-39 0.22023602265849718 1103 40-41 0.13668963828872904 1103 42-43 0.04817340466223641 1103 44-45 0.16006240821645434 1103 46-47 0.28881311748478566 1103 48-49 -0.0619634837370171 1103 50-51 -0.21046341138170988 1103 52-53 -0.07573235520077048 1103 54-55 -0.029778381410800137 1103 56-57 -0.16665949663414636 1103 58-59 0.3565516009413443 1103 60-61 0.0026724409756511136 1103 62-63 -0.11672991162821944 1103 64-65 -0.0057535357591902425 1103 66-67 0.03664210550507363 1103 68-69 0.26979808703375596 1103 70-71 -0.0037388985939159625 1103 72-73 0.3466924221450647 1103 74-75 0.2657406701053553 1103 76-77 0.10848507950622022 1103 78-79 0.08833623636678922 1103 80-81 0.6409246119987273 1103 82-83 0.7025142891817211 1103 84-85 0.8693300841865614 1103 86-87 0.3950186047509838 1103 88-89 0.24605658413557308 1103 90-91 0.5574205623089199 1103 92-93 0.5421290428956382 1103 94-95 0.8487209523874277 1103 96-97 0.4758951267536524 1103 98-99 0.3709718769595405 1103 100-101 0.7160716526599558 1104 1 0.017860164698188186 1104 2 0.040543033660519257 1104 3 0.056753559065867876 1104 4 0.13116177554834252 1104 5 0.3275848353294961 1104 6 0.13510314387427513 1104 7 0.0809396624179044 1104 8 0.13046903033849588 1104 9 0.07066399833325221 1104 10-11 -0.1650714194059688 1104 12-13 0.04533829743286333 1104 14-15 0.1671468345665872 1104 16-17 -0.0162408847243114 1104 18-19 0.12668751033936587 1104 20-21 -0.017817976053159157 1104 22-23 0.13619807457516941 1104 24-25 -0.01256906142772607 1104 26-27 0.14122085081319113 1104 28-29 0.26342385342918107 1104 30-31 0.07275278525714413 1104 32-33 -0.1791614613094481 1104 34-35 -0.05698842860498132 1104 36-37 0.2880825189246181 1104 38-39 -0.05918979832873106 1104 40-41 0.06911038428904703 1104 42-43 0.4199503328159011 1104 44-45 0.29705291821850466 1104 46-47 0.4035351310939461 1104 48-49 0.23744936531057448 1104 50-51 0.04268285292725693 1104 52-53 0.04569751667288102 1104 54-55 -0.0654455259910307 1104 56-57 -0.24707983299426672 1104 58-59 -0.07078277925027265 1104 60-61 -0.18432831003138261 1104 62-63 -0.3058414475513942 1104 64-65 -0.23153355897213856 1104 66-67 -0.22973212357482709 1104 68-69 -0.28653312982520873 1104 70-71 -0.7043146706519039 1104 72-73 -0.36857350261768573 1104 74-75 -0.2288998706197063 1104 76-77 -0.4539053986349266 1104 78-79 -0.37940459793374615 1104 80-81 -0.3104419932219109 1104 82-83 -0.157183942538083 1104 84-85 -0.6567617207336731 1104 86-87 -0.540815809577321 1104 88-89 -0.25595179073072316 1104 90-91 -0.4266091593633199 1104 92-93 -0.26844650158446015 1104 94-95 -0.09566470110247494 1104 96-97 0.42777378222348617 1104 98-99 0.3385249595020383 1104 100-101 0.951808208496189 1105 1 -0.16571860839347963 1105 2 0.023463912015351696 1105 3 0.03207161413732251 1105 4 0.023872650417636976 1105 5 0.022143043764486947 1105 6 -0.06821247132265995 1105 7 -0.08251014587766292 1105 8 -0.14209980897834384 1105 9 -0.2214020525555611 1105 10-11 -0.07803107433799994 1105 12-13 0.038639534797802355 1105 14-15 0.012901017208626797 1105 16-17 -0.027252996958544884 1105 18-19 -0.11233305431731821 1105 20-21 0.004282494494070477 1105 22-23 -0.07290135031154676 1105 24-25 -0.043274446579346204 1105 26-27 0.07055162808750026 1105 28-29 0.017726310731376316 1105 30-31 -1.929275347762882E-4 1105 32-33 0.09958331038799884 1105 34-35 0.1745818049258574 1105 36-37 -0.009614849496436761 1105 38-39 0.07100582732481087 1105 40-41 0.081571172019963 1105 42-43 0.060014379522073114 1105 44-45 -0.2652292358421491 1105 46-47 -0.08247915959968566 1105 48-49 0.11476942456466332 1105 50-51 0.06805101256475865 1105 52-53 0.34494159126333557 1105 54-55 0.42236599370224326 1105 56-57 0.20273935487606565 1105 58-59 0.12667278017810446 1105 60-61 -0.22838765126525828 1105 62-63 -0.4043949505140958 1105 64-65 -0.06119981899305316 1105 66-67 0.25500776837358785 1105 68-69 -0.0666721155345229 1105 70-71 -0.1530990903103202 1105 72-73 -0.056607225169098996 1105 74-75 0.29266687737611363 1105 76-77 0.06379458393739768 1105 78-79 0.44334505351275055 1105 80-81 0.047304608381434576 1105 82-83 0.4449858030178575 1105 84-85 0.07449263618511637 1105 86-87 0.09385655012989602 1105 88-89 0.11152163206467591 1105 90-91 0.0526954763982701 1105 92-93 0.1936483049683453 1105 94-95 0.2899117192530447 1105 96-97 -0.20006910728505645 1105 98-99 -0.0023494740985157136 1105 100-101 1.0403843674365874 1106 1 -0.039238345235585115 1106 2 -0.25235845640570176 1106 3 -0.14490207007320777 1106 4 -0.15803524431920835 1106 5 0.009807517448699343 1106 6 -0.387620366059501 1106 7 -0.09780619850924666 1106 8 0.11563045417955209 1106 9 0.09224926323391003 1106 10-11 0.02912353092515474 1106 12-13 -0.022873623096934637 1106 14-15 -0.007904903844000444 1106 16-17 0.058355555673031745 1106 18-19 0.12993668252479296 1106 20-21 0.022703547125644263 1106 22-23 0.22964799179371198 1106 24-25 0.21708739552591538 1106 26-27 0.19481149650855656 1106 28-29 0.30645986336295294 1106 30-31 0.30219194088628143 1106 32-33 0.11816883670379497 1106 34-35 0.4744995680837576 1106 36-37 0.2037075189246167 1106 38-39 0.21023280100901331 1106 40-41 0.47408761938838495 1106 42-43 0.5190604321536512 1106 44-45 0.30204050099995783 1106 46-47 0.607487945663479 1106 48-49 0.3807233719330867 1106 50-51 0.6892681178279219 1106 52-53 0.5854843544212258 1106 54-55 0.7553429673864613 1106 56-57 0.5629367232971205 1106 58-59 0.6737122538623126 1106 60-61 0.9952143224189527 1106 62-63 0.7187136021174823 1106 64-65 0.5310699178490594 1106 66-67 0.9366689525841139 1106 68-69 0.8260910423602112 1106 70-71 0.9117035412686221 1106 72-73 1.1893631695677378 1106 74-75 1.0633083247445256 1106 76-77 0.5583669523584511 1106 78-79 0.7769799219338047 1106 80-81 0.937353950486699 1106 82-83 1.092929881965226 1106 84-85 0.7329630309219581 1106 86-87 1.1575078659193672 1106 88-89 1.3203209741699382 1106 90-91 0.9901954763982701 1106 92-93 1.2527765944420288 1106 94-95 1.6666386929372585 1106 96-97 0.8565920769254696 1106 98-99 1.384986052217272 1106 100-101 2.0403843674365874 1107 1 -0.07260852208446167 1107 2 -0.052268022322103036 1107 3 0.18451915179174705 1107 4 -0.1076534115217811 1107 5 -0.17513018351593246 1107 6 0.17003638635207352 1107 7 0.1465366471499152 1107 8 -0.012213195338134142 1107 9 -0.1754657689204322 1107 10-11 0.19448225279010956 1107 12-13 -0.029947578080857795 1107 14-15 0.19525526496628487 1107 16-17 0.21023959908139744 1107 18-19 0.06324656998459943 1107 20-21 -0.053240986636410526 1107 22-23 0.10469320883551347 1107 24-25 -0.06403197746443823 1107 26-27 -0.09648171088694824 1107 28-29 -0.2088084244183932 1107 30-31 -0.2593685494674176 1107 32-33 -0.1909851022029585 1107 34-35 0.0026195439679952415 1107 36-37 0.29853267969632213 1107 38-39 0.3221851723916558 1107 40-41 -0.12045116678524437 1107 42-43 -0.31764575755696 1107 44-45 -0.48173160510936697 1107 46-47 -0.9770679225037213 1107 48-49 -0.6984868370701278 1107 50-51 -1.0780449850659721 1107 52-53 -0.8164700266077105 1107 54-55 -1.111020577629624 1107 56-57 -0.5803510580533597 1107 58-59 -0.8292419262019948 1107 60-61 -1.0348851550730132 1107 62-63 -0.9089642515802652 1107 64-65 -1.090001223630047 1107 66-67 -1.0460531133322846 1107 68-69 -0.9303599222700072 1107 70-71 -1.115687937831055 1107 72-73 -1.0308890410431921 1107 74-75 -1.0311400273455007 1107 76-77 -1.2803890765804518 1107 78-79 -0.9573096680983504 1107 80-81 -0.5663990639827539 1107 82-83 -0.48429580131451644 1107 84-85 -0.6001509160233773 1107 86-87 -0.6349258157526556 1107 88-89 -0.49966998242170035 1107 90-91 -0.9959379641162016 1107 92-93 -0.8306789923746898 1107 94-95 -0.7597529871270545 1107 96-97 -0.882962786418581 1107 98-99 -0.4583117773647203 1107 100-101 0.3960917629349794 1108 1 0.014198181244164942 1108 2 0.06289660589959567 1108 3 0.15712285204517684 1108 4 0.06776304228515784 1108 5 0.07956024330539435 1108 6 -0.05235946263270819 1108 7 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2316 76-77 -2.6948267289602263 2316 78-79 -2.3695345011431215 2316 80-81 -2.7145519561067104 2316 82-83 -3.039470530122685 2316 84-85 -2.934670897649468 2316 86-87 -2.5623753758388723 2316 88-89 -2.949442075280608 2316 90-91 -3.1821946334918394 2316 92-93 -4.002563378085444 2316 94-95 -3.6817301257440604 2316 96-97 -4.051048719777828 2316 98-99 -4.837026310420093 2316 100-101 -5.123077171024953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 4.0 5 18.0 6 39.0 7 44.0 8 89.0 9 159.0 10 269.0 11 393.0 12 526.0 13 700.0 14 917.0 15 1165.0 16 1526.0 17 1803.0 18 1914.0 19 1930.0 20 1972.0 21 2312.0 22 2506.0 23 2805.0 24 3100.0 25 3699.0 26 4294.0 27 5008.0 28 6187.0 29 7600.0 30 9435.0 31 11745.0 32 14460.0 33 18509.0 34 24478.0 35 35733.0 36 56065.0 37 71948.0 38 26933.0 39 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.43930650325507 17.899772552115643 14.828599535078979 12.832321409550303 2 14.120217214905436 30.417264839897634 25.788028212970477 29.67448973222645 3 37.895886648773484 20.400411959303412 31.74989076836652 9.953810623556581 4 12.173085325510268 15.535235004057174 44.280007490169154 28.011672180263403 5 8.909299727231303 44.59143993158936 33.840795460929165 12.658464880250172 6 24.069658573122776 37.11066724923538 11.49647337869047 27.323200798951376 7 20.108295362336932 35.6179389551214 22.99856438424568 21.275201298295986 8 23.084077148742278 16.868173022907435 18.72323824979714 41.32451157855315 9 13.193308782223331 29.633605892266402 31.921852568503837 25.25123275700643 10-11 28.360121091067974 20.06023344360527 24.02237688034455 27.557268584982207 12-13 38.5515885400412 20.404313089070595 22.14374882966107 18.90034954122714 14-15 25.114733837782506 26.925819755910684 33.990864986244425 13.968581420062387 16-17 17.349876643004386 28.782362680490557 32.07235071150692 21.795409964998136 18-19 16.95711878159915 38.584514075276196 24.63251357593159 19.825853567193057 20-21 24.40484364271893 25.05758067536358 19.124898570626055 31.41267711129143 22-23 18.441888770988076 25.72046064540291 27.14140815180076 28.69624243180825 24-25 31.688877098807815 26.100898820298358 20.812995443480432 21.397228637413395 26-27 25.731851944323076 18.178484489108047 37.36143187066975 18.728231695899133 28-29 16.750670994319954 38.52802571624743 24.592409961924975 20.128893327507647 30-31 21.06641283315648 25.22376880344548 23.077367205542725 30.632451157855318 32-33 23.08314087759815 26.4215716871606 28.950284002247052 21.545003432994196 34-35 30.09674952795593 22.653589875630043 24.567357957648674 22.68230263876535 36-37 23.289699965979707 20.98412282262318 36.292162535853954 19.434014675543153 38-39 21.843699572074673 27.917835840228726 30.619133974024837 19.619330613671764 40-41 20.594477179666434 30.96661839608509 26.573111205432937 21.86579321881554 42-43 18.75993814358989 30.69522922294055 19.504743000972162 31.0400896324974 44-45 24.554605929432913 20.84289764791983 23.9541412246796 30.648355197967657 46-47 30.70354754243662 29.145587496371938 25.884388878312457 14.266476082878981 48-49 25.24596661563981 24.905631523674433 28.468929013371536 21.379472847314222 50-51 12.05985297353305 29.22162734049376 38.16056189015303 20.557957795820155 52-53 21.218088758504464 34.003963547843455 20.99853317520754 23.77941451844454 54-55 16.389894513451093 23.379626739903873 27.945196929030647 32.28528181761438 56-57 26.720866362898693 20.880719056238686 25.034798077523252 27.363616503339365 58-59 34.713243107863285 21.117628608745722 23.75058321981028 20.418545063580712 60-61 22.772555335006178 26.83888243885997 33.93490256295021 16.45365966318365 62-63 17.50327694900443 27.577242369390177 31.99753448598714 22.92194619561825 64-65 16.817510821767748 36.93507563533998 24.94062461963866 21.306788923253613 66-67 22.92031103461298 24.986228775539185 20.282165757167668 31.81129443268017 68-69 17.82420014013055 25.823188953093716 27.236666141307147 29.115944765468587 70-71 29.299513139005057 25.865114537169966 21.243836214967853 23.591536108857124 72-73 23.519599275950316 18.786904687597527 35.80612945509019 21.88736658136196 74-75 16.30999937581924 36.22292615941577 24.458367143124647 23.008707321640347 76-77 20.575102334156174 24.036340343092835 23.89713464362716 31.491422679123833 78-79 21.888770988078146 25.18272891829474 28.702796329817115 24.22570376381 80-81 27.60891954309968 22.62920541788902 24.860963735097684 24.900911303913613 82-83 21.1480244678859 21.37553835590787 34.251763310654766 23.224673865551466 84-85 19.94148305349229 26.63675800511828 29.59100555520879 23.83075338618064 86-87 19.574454120834904 28.94374549756146 26.437537797043742 25.044262584559895 88-89 18.46512823245129 28.00679476852969 20.95643888939383 32.57163810962519 90-91 22.875310537858613 20.230240243201823 24.755522368900973 32.13892685003859 92-93 26.797172461144747 27.095062730166656 26.38505711253979 19.722707696148806 94-95 22.60548654890456 23.63897384682604 28.06207477685538 25.69346482741402 96-97 12.479206783660043 26.76278100100806 35.05946937896554 25.69854283636636 98-99 20.147306660008738 29.661537981399412 22.346139441982398 27.84501591660945 100-101 17.692018407176864 22.873679266334495 26.446194586142813 32.98810774034583 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2476.0 1 1428.5 2 348.0 3 268.0 4 199.0 5 190.5 6 313.0 7 625.0 8 742.0 9 598.5 10 391.0 11 208.0 12 164.5 13 154.5 14 149.5 15 137.5 16 118.0 17 112.5 18 127.5 19 130.5 20 132.0 21 150.0 22 168.5 23 179.0 24 201.0 25 242.5 26 291.0 27 338.5 28 358.0 29 393.5 30 466.5 31 525.0 32 597.0 33 672.0 34 692.5 35 694.5 36 735.5 37 758.0 38 705.5 39 615.5 40 580.0 41 543.0 42 537.0 43 614.5 44 809.5 45 2077.0 46 8320.5 47 39978.5 48 92025.5 49 85408.5 50 38510.0 51 17905.0 52 9063.5 53 3750.0 54 999.5 55 467.5 56 360.0 57 329.0 58 304.5 59 255.5 60 200.0 61 185.5 62 151.5 63 106.0 64 73.0 65 51.0 66 38.5 67 38.0 68 35.5 69 31.5 70 26.5 71 18.0 72 14.0 73 11.0 74 11.0 75 10.5 76 6.5 77 4.0 78 4.0 79 3.0 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38262280756507083 2 0.0 3 0.0 4 0.0 5 6.24180762748892E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001404406716185007 16-17 0.002652768241682791 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015604519068722301 36-37 0.0071780787716122585 38-39 0.011547344110854504 40-41 0.001248361525497784 42-43 1.56045190687223E-4 44-45 4.681355720616691E-4 46-47 3.12090381374446E-4 48-49 1.56045190687223E-4 50-51 4.681355720616691E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.56045190687223E-4 60-61 0.002496723050995568 62-63 0.0 64-65 3.12090381374446E-4 66-67 0.0017164970975594531 68-69 0.001404406716185007 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12171524873603395 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14184507833468574 88-89 0.42163410523687656 90-91 0.3166156919043755 92-93 0.0 94-95 0.0 96-97 9.362711441233382E-4 98-99 0.0 100-101 4.681355720616691E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 320420.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.961987391548593 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8405070344059 7.155296173771925 2 9.043738682267726 1.6209974408588728 3 3.1132469703301293 0.8370264028462643 4 1.8421785764033989 0.6603832469883278 5 1.1770441565677672 0.5274327445228137 6 0.7661234155174815 0.41195930341426873 7 0.38306170775874077 0.24030959365832347 8 0.4004736035659563 0.28712315086449036 9 0.2785903329154478 0.22470507458960115 >10 2.3262292798439894 4.215716871606017 >50 0.320378882852765 2.0972473628362773 >100 0.3308260203370943 6.309843330628551 >500 0.05571806658308956 3.6617564446663753 >1k 0.06268282490597576 12.468322826290494 >5k 0.03134141245298788 19.767180575494663 >10k+ 0.02785903329154478 39.51469945696274 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36336 11.340116097621872 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 22228 6.937144997191187 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14548 4.540290868235441 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11056 3.4504712564758755 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11043 3.4464140815180073 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10724 3.3468572498595592 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10472 3.268210473753199 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 10206 3.185194432307596 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9591 2.9932588477623123 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8578 2.6771112914299984 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8138 2.5397915236252415 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7442 2.3225766181886276 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6946 2.167779789026902 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5896 1.8400848885837338 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5780 1.803882404344298 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5775 1.8023219524374259 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5192 1.6203732600961238 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4280 1.3357468322826291 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3627 1.1319518132451158 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3565 1.1126022095999002 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3516 1.0973097809125523 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3121 0.9740340802696461 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2168 0.676611946819799 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2110 0.6585107047000812 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2075 0.6475875413519756 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1931 0.6026465264340553 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1920 0.5992135322389365 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 0.5642594095249984 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1616 0.5043380563011047 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1612 0.5030896947756069 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1486 0.4637663067224268 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1400 0.4369265339242244 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1356 0.4231945571437489 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1249 0.3898008863366831 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1111 0.3467324137070096 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 988 0.3083452967979527 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 963 0.30054303726359155 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 800 0.24967230509955682 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 785 0.24499094937894014 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 773 0.2412458648024468 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 756 0.23594032831908118 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 752 0.2346919667935834 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 741 0.2312589725984645 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 738 0.23032270145434117 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 724 0.22595343611509894 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 692 0.21596654391111667 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 0.21409400162286998 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 670 0.20910055552087886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 613 0.19131140378253542 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 533 0.16634417327257975 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 519 0.1619749079333375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.15573310030584855 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 498 0.1554210099244741 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 457 0.14262530428812184 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 454 0.1416890331439985 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 450 0.1404406716185007 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 418 0.13045377941451844 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 417 0.130141689033144 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 402 0.1254603333125273 RNA PCR Primer, Index 27 (95% over 23bp) GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 401 0.12514824293115284 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 384 0.11984270644778729 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 375 0.11703389301541726 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 367 0.1145371699644217 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 335 0.10455027776043943 No Hit TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT 330 0.10298982585356718 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.12090381374446E-4 0.0 16-17 0.0 0.0 0.0 6.24180762748892E-4 0.0 18-19 0.0 0.0 0.0 0.0017164970975594531 0.0 20-21 0.0 0.0 0.0 0.0034329941951189063 0.0 22-23 0.0 0.0 0.0 0.007022033580925036 0.0 24-25 0.0 0.0 0.0 0.011235253729480057 0.0 26-27 0.0 0.0 0.0 0.01919355845452843 0.0 28-29 0.0 0.0 0.0 0.06351039260969976 0.0 30-31 0.0 0.0 0.0 0.15854191373821858 0.0 32-33 0.0 0.0 0.0 0.36062043567817237 0.0 34-35 0.0 0.0 0.0 0.6112290119218526 0.0 36-37 0.0 0.0 0.0 0.8981961175956557 0.0 38-39 0.0 0.0 0.0 1.3029773422383122 0.0 40-41 0.0 0.0 0.0 1.6753011672180262 0.0 42-43 0.0 0.0 0.0 2.0988078147431497 0.0 44-45 0.0 0.0 0.0 2.5719368329068097 0.0 46-47 0.0 0.0 0.0 3.1447787279196056 0.0 48-49 0.0 0.0 0.0 3.5782722676487113 0.0 50-51 0.0 0.0 0.0 4.0351725859809 0.0 52-53 0.0 0.0 0.0 4.46055177579427 0.0 54-55 0.0 0.0 0.0 4.9879845203170845 0.0 56-57 3.12090381374446E-4 0.0 0.0 5.790837026402846 0.0 58-59 3.12090381374446E-4 0.0 0.0 6.334966606329193 0.0 60-61 3.12090381374446E-4 0.0 0.0 7.0031521128518825 0.0 62-63 3.12090381374446E-4 0.0 0.0 7.644341801385681 0.0 64-65 3.12090381374446E-4 0.0 0.0 8.28334685724986 0.0 66-67 3.12090381374446E-4 0.0 0.0 9.13738218588103 0.0 68-69 3.12090381374446E-4 0.0 0.0 10.13544722551651 0.0 70-71 3.12090381374446E-4 0.0 0.0 11.062355658198614 0.0 72-73 3.12090381374446E-4 0.0 0.0 11.802321952437426 0.0 74-75 3.12090381374446E-4 0.0 0.0 12.883871169090568 0.0 76-77 3.12090381374446E-4 0.0 0.0 13.623525372948006 0.0 78-79 3.12090381374446E-4 0.0 0.0 15.149179202296985 0.0 80-81 3.12090381374446E-4 0.0 0.0 16.525965919730353 0.0 82-83 3.12090381374446E-4 0.0 0.0 17.46504587728606 0.0 84-85 3.12090381374446E-4 0.0 0.0 18.661912489857063 0.0 86-87 3.12090381374446E-4 0.0 0.0 19.773266337931467 0.0 88-89 3.12090381374446E-4 0.0 0.0 21.079676674364897 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 170 0.0 41.878513 94-95 GCTTTGA 50 0.0015920022 38.106625 1 AGACGGC 25 0.0016215931 37.96985 94-95 CTTTGAT 50 0.0016203828 37.96985 2 CTCCGAC 60 3.8180588E-9 35.59673 94-95 AAGCAGG 315 0.0 31.64154 94-95 TCAACGA 60 1.5145633E-7 31.641539 94-95 CCACTGT 70 1.7165803E-8 30.511488 94-95 CATGGGT 55 2.747911E-6 30.203289 46-47 ACCACTC 145 0.0 29.459368 94-95 GCTTTGC 900 0.0 28.579967 1 ATGGGTA 50 4.9765884E-5 28.477386 48-49 TGGGTAC 50 4.9765884E-5 28.477386 48-49 CCGCAGA 35 0.008183668 27.280972 88-89 AGTAACC 35 0.0084213875 27.121323 94-95 CTTTGCG 945 0.0 27.12132 2 TTTGCGT 945 0.0 27.12132 3 CACCGCA 45 8.666112E-4 26.531494 86-87 GCAGAGA 745 0.0 26.120197 94-95 GAGAAAA 75 1.066237E-6 25.313234 12-13 >>END_MODULE