FastQCFastQC Report
Fri 27 May 2016
SRR938947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163249
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99950.462036501577026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86600.40032377225183047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71610.3310298537061614No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58330.26964071172574217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.20857515709009922No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42920.1984052691114153No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36470.1685890066284556No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28460.1315613690333383No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25340.1171386188090229No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24970.11542822855806244No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24310.11237726216445725No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21860.10105170509728653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG89150.022.4771542
GAGTACT72550.022.38556112-13
GTACATG91000.022.3775921
ACATGGG91650.020.9336433
AGTACTT78050.020.5351112-13
GTACTTT80350.020.06538614-15
AGAGTAC131400.019.72899810-11
CATGGGG47150.017.98434
CATGGGA47700.017.4789984
TTTGCGT5700.017.4528643
TACTTTT89350.017.43482814-15
GTATCAA224950.017.3664591
CATACCG1650.001491068617.2262025
ACTTTTT95900.016.98738316-17
GGTATCA185750.016.7382451
CCGTTGC2555.680118E-616.711439
TATTCCG2901.0597068E-616.3351925
CAACGCA249600.015.5249785
TATCAAC252600.015.4156092
AACGCAG252100.015.4011186
Produced by FastQC (version 0.11.5)