##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938947_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2163249 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.586070304435598 33.0 31.0 34.0 30.0 34.0 2 31.672524059874753 33.0 31.0 34.0 30.0 34.0 3 31.708194479692352 33.0 31.0 34.0 28.0 34.0 4 35.25416768943381 37.0 35.0 37.0 33.0 37.0 5 35.291242940595374 37.0 35.0 37.0 33.0 37.0 6 35.37726262672489 37.0 35.0 37.0 33.0 37.0 7 35.29123092163685 37.0 35.0 37.0 32.0 37.0 8 35.275003016296324 37.0 35.0 37.0 32.0 37.0 9 36.83725590535347 39.0 37.0 39.0 33.0 39.0 10-11 36.71482732685881 39.0 37.0 39.0 32.0 39.0 12-13 36.59637286322564 39.0 37.0 39.0 32.0 39.0 14-15 37.73925724685415 40.0 37.5 41.0 32.0 41.0 16-17 37.65221756718714 40.0 37.0 41.0 32.0 41.0 18-19 37.57629750435572 40.0 37.0 41.0 32.0 41.0 20-21 37.67880188549723 40.0 38.0 41.0 32.0 41.0 22-23 37.60365889456091 40.0 37.0 41.0 32.0 41.0 24-25 37.50662799335629 40.0 37.0 41.0 32.0 41.0 26-27 37.37000849185647 40.0 37.0 41.0 31.0 41.0 28-29 37.22866738872872 40.0 37.0 41.0 31.0 41.0 30-31 37.018846882628864 40.0 36.5 41.0 30.5 41.0 32-33 36.7901866590485 39.5 36.0 41.0 30.0 41.0 34-35 36.44154140369417 39.0 35.5 41.0 30.0 41.0 36-37 36.163242419157484 39.0 35.0 41.0 29.5 41.0 38-39 35.90183700535629 39.0 35.0 40.5 28.0 41.0 40-41 35.73514237149769 39.0 35.0 40.0 27.5 41.0 42-43 35.502162487998376 39.0 35.0 40.0 26.5 41.0 44-45 35.414136791465054 39.0 35.0 40.5 26.0 41.0 46-47 35.166932470557015 39.0 35.0 41.0 24.5 41.0 48-49 34.88439865221248 39.0 34.5 40.5 24.0 41.0 50-51 33.75903398083161 38.0 33.0 39.5 22.5 40.5 52-53 33.864424992222354 38.0 33.5 39.5 23.0 40.5 54-55 34.347752847684205 38.0 34.0 40.0 23.0 41.0 56-57 34.22086639124761 38.0 34.0 40.0 22.5 41.0 58-59 33.98842551181117 38.0 34.0 40.0 22.0 41.0 60-61 33.63963787802514 37.5 33.0 40.0 20.5 41.0 62-63 33.2457645883576 37.0 33.0 40.0 19.5 41.0 64-65 32.83579121035073 36.0 32.0 39.5 18.0 41.0 66-67 32.392969556440335 36.0 32.0 39.0 14.5 41.0 68-69 31.92405127657519 35.0 31.0 39.0 11.5 40.0 70-71 31.44622741071416 35.0 31.0 38.0 8.0 40.0 72-73 30.993861316935778 35.0 30.5 37.0 7.0 39.5 74-75 30.525400913163487 35.0 30.0 37.0 2.0 39.0 76-77 30.093638781296097 34.0 30.0 36.0 2.0 39.0 78-79 29.690731164096228 34.0 29.0 36.0 2.0 38.0 80-81 29.223187899312563 34.0 29.0 35.0 2.0 37.0 82-83 28.745473590881126 34.0 29.0 35.0 2.0 37.0 84-85 28.318849563781146 34.0 28.0 35.0 2.0 36.0 86-87 27.866912454368403 34.0 27.0 35.0 2.0 36.0 88-89 27.362365358772845 33.5 26.0 35.0 2.0 35.5 90-91 26.879799782641758 33.0 25.0 35.0 2.0 35.0 92-93 26.33323533259463 33.0 24.5 35.0 2.0 35.0 94-95 25.75399965514834 32.5 22.5 35.0 2.0 35.0 96-97 25.059239366342016 32.0 19.0 34.5 2.0 35.0 98-99 24.145689654773907 32.0 2.0 34.0 2.0 35.0 100-101 21.06279536012729 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24797206671751226 1101 2 0.5093240667099188 1101 3 0.44738296188541327 1101 4 0.4303929481786497 1101 5 0.29182831776545726 1101 6 0.519818939098009 1101 7 0.5378660935947224 1101 8 0.4769496592226332 1101 9 0.6508843060603766 1101 10-11 0.5130289271558226 1101 12-13 0.5291339757354123 1101 14-15 0.7717679570977936 1101 16-17 0.6481199283216554 1101 18-19 0.7623053505679138 1101 20-21 0.6681968466473549 1101 22-23 0.729257290635033 1101 24-25 0.8720258434057726 1101 26-27 0.8242317386235598 1101 28-29 0.7611710320022382 1101 30-31 0.8082213573886463 1101 32-33 0.7547995537855314 1101 34-35 0.6350165186073511 1101 36-37 0.6704400775409454 1101 38-39 0.7467051675227694 1101 40-41 0.6495992967285602 1101 42-43 0.5800342479877401 1101 44-45 0.7511467403349812 1101 46-47 0.620790123865838 1101 48-49 0.7579655761607498 1101 50-51 0.5751521168033449 1101 52-53 0.6368024946864921 1101 54-55 0.6988334667871428 1101 56-57 0.7735421292964517 1101 58-59 0.611595201778016 1101 60-61 0.7527615631762217 1101 62-63 0.578244233647716 1101 64-65 0.5125667012369703 1101 66-67 0.6055625832811842 1101 68-69 0.6230987050790802 1101 70-71 0.6210228588171667 1101 72-73 0.4547005668302688 1101 74-75 0.22820734608597704 1101 76-77 0.3046409056814774 1101 78-79 0.21615509786218468 1101 80-81 0.1698139532330707 1101 82-83 0.3154097936062321 1101 84-85 0.19960383936263426 1101 86-87 0.16949891098233394 1101 88-89 -0.04115676900994458 1101 90-91 0.10989751955002092 1101 92-93 0.044385425548647106 1101 94-95 -0.3335205198700031 1101 96-97 -0.40889780982999113 1101 98-99 -0.45457898973070243 1101 100-101 -0.3434200957049356 1102 1 0.4326451232894932 1102 2 0.43718725765154076 1102 3 0.531177501076872 1102 4 0.4578907313885665 1102 5 0.5625380563797364 1102 6 0.49336427702763785 1102 7 0.6012225219075731 1102 8 0.5274377314414309 1102 9 0.6494683353333528 1102 10-11 0.6713718150751689 1102 12-13 0.6906112723993161 1102 14-15 0.8052179635936483 1102 16-17 0.7740962934922635 1102 18-19 0.6544280831668985 1102 20-21 0.7110362047305259 1102 22-23 0.7297794818705441 1102 24-25 0.7277875425706455 1102 26-27 0.7638114377455452 1102 28-29 0.7356747295955017 1102 30-31 0.7419995623373481 1102 32-33 0.6836407811619694 1102 34-35 0.695885348962058 1102 36-37 0.7150318034586647 1102 38-39 0.8562492844835177 1102 40-41 0.6322650703725401 1102 42-43 0.4880297021490634 1102 44-45 0.6545191623310913 1102 46-47 0.7526509788215492 1102 48-49 0.6879417496089744 1102 50-51 0.7804528623763858 1102 52-53 0.8010400891759915 1102 54-55 0.8717649146821671 1102 56-57 0.6662113968286434 1102 58-59 0.743450128475736 1102 60-61 0.6791225083171284 1102 62-63 0.5595236778546067 1102 64-65 0.4310371349962523 1102 66-67 0.445854304027435 1102 68-69 0.7401458523887285 1102 70-71 0.5038446591660524 1102 72-73 0.42236065817589363 1102 74-75 0.40014423410370625 1102 76-77 0.5729696288482309 1102 78-79 0.4538680263837911 1102 80-81 0.4047033912215987 1102 82-83 0.612616953428283 1102 84-85 0.46396920673173625 1102 86-87 0.250427957302211 1102 88-89 0.22411839676054512 1102 90-91 0.3202766379549935 1102 92-93 0.2767413531129037 1102 94-95 0.2537153100167444 1102 96-97 0.29275604801542343 1102 98-99 0.08851881517410831 1102 100-101 0.07367586513324653 1103 1 0.24007016377517232 1103 2 0.22671426024447783 1103 3 0.3538973831947807 1103 4 0.3133983233769655 1103 5 0.10048048024525258 1103 6 0.2657815582266352 1103 7 0.23166099855976796 1103 8 0.14495582580767064 1103 9 0.37819761668652063 1103 10-11 0.35875014217746326 1103 12-13 0.32937724925984924 1103 14-15 0.4423645131323326 1103 16-17 0.4837091500084014 1103 18-19 0.5145202986791162 1103 20-21 0.5616609893512035 1103 22-23 0.4298663468409387 1103 24-25 0.5702869112917952 1103 26-27 0.619662009975734 1103 28-29 0.5112069871312457 1103 30-31 0.6598699966396779 1103 32-33 0.5600133590595249 1103 34-35 0.5376059110342126 1103 36-37 0.6124055082734259 1103 38-39 0.727645833459114 1103 40-41 0.6681901547787135 1103 42-43 0.6862630223104205 1103 44-45 0.8220060118354624 1103 46-47 0.9159094487818109 1103 48-49 0.9756556242546353 1103 50-51 0.7112211556361316 1103 52-53 0.8612280538604082 1103 54-55 0.995586537928439 1103 56-57 0.8382026617813167 1103 58-59 0.9435238548378138 1103 60-61 1.0337867267814076 1103 62-63 0.8810266745834667 1103 64-65 0.7020239345739228 1103 66-67 0.7138075866399092 1103 68-69 0.7632551628570425 1103 70-71 0.780976198157564 1103 72-73 0.7749486011378188 1103 74-75 0.49236587767103046 1103 76-77 0.47652636273535265 1103 78-79 0.3585364116638452 1103 80-81 0.43289171227512924 1103 82-83 0.5085469128837907 1103 84-85 0.42034577376103854 1103 86-87 0.6162358428196342 1103 88-89 0.5154302480104782 1103 90-91 0.56661228009634 1103 92-93 0.3708983762496132 1103 94-95 0.2700721265895112 1103 96-97 0.2686892458250547 1103 98-99 0.16637045000488726 1103 100-101 0.1866448727829173 1104 1 0.16157503966712738 1104 2 0.20475229244667403 1104 3 0.2594216793687778 1104 4 0.2852369547468001 1104 5 0.06895216185845499 1104 6 0.22596361112289998 1104 7 0.18508759562566013 1104 8 0.19584171695316854 1104 9 0.21230509942309084 1104 10-11 0.3178037953608239 1104 12-13 0.3469025350647712 1104 14-15 0.41830239664493263 1104 16-17 0.4785062389257533 1104 18-19 0.469560557550885 1104 20-21 0.3912717753484145 1104 22-23 0.4764053331551281 1104 24-25 0.49499706456211356 1104 26-27 0.5348185504807859 1104 28-29 0.544677211243453 1104 30-31 0.3797665998050306 1104 32-33 0.5480715268215661 1104 34-35 0.6408587102934646 1104 36-37 0.6386277881968994 1104 38-39 0.8083895508949936 1104 40-41 0.7591749600247368 1104 42-43 0.5693480497334846 1104 44-45 0.6323022152291102 1104 46-47 0.7002689934535979 1104 48-49 0.6867098506400566 1104 50-51 0.4017444597296347 1104 52-53 0.501494396855712 1104 54-55 0.69593829783026 1104 56-57 0.728857140963548 1104 58-59 0.6544307388966715 1104 60-61 0.7278371832609167 1104 62-63 0.8221125250659114 1104 64-65 0.6821155734174269 1104 66-67 0.6391162951470548 1104 68-69 0.7025602900760575 1104 70-71 0.6768823350940671 1104 72-73 0.6677288271362052 1104 74-75 0.6217817994607948 1104 76-77 0.6583095600760878 1104 78-79 0.6197114024166765 1104 80-81 0.5068591524379791 1104 82-83 0.6806331969421571 1104 84-85 0.5929665565003077 1104 86-87 0.7736731396574683 1104 88-89 0.5080697455523264 1104 90-91 0.4077844783262705 1104 92-93 0.07904103007720664 1104 94-95 0.17052407437423156 1104 96-97 0.3197341148091901 1104 98-99 0.23269090915443869 1104 100-101 0.3541514192894475 1105 1 0.311051640652348 1105 2 0.38351555504614154 1105 3 0.49837582866775065 1105 4 0.38091950495520877 1105 5 0.374489297136968 1105 6 0.39482255390425536 1105 7 0.35700167292766594 1105 8 0.47159010649465927 1105 9 0.45738372769137214 1105 10-11 0.4588929863422564 1105 12-13 0.40658541683816907 1105 14-15 0.6696322090738818 1105 16-17 0.5637883059966171 1105 18-19 0.5568024105254992 1105 20-21 0.5883078211991162 1105 22-23 0.4981157348224201 1105 24-25 0.5138003517921206 1105 26-27 0.44362396919999725 1105 28-29 0.49742091150870493 1105 30-31 0.4613481647917652 1105 32-33 0.4865520118537745 1105 34-35 0.6765765484897557 1105 36-37 0.6625182296519938 1105 38-39 0.7163293481666884 1105 40-41 0.7634012294449732 1105 42-43 0.7333460129771368 1105 44-45 0.8053483501287033 1105 46-47 0.684754120395624 1105 48-49 0.8205661882679465 1105 50-51 0.533098665868323 1105 52-53 0.7161649633581746 1105 54-55 0.6719850958598172 1105 56-57 0.6274858829112588 1105 58-59 0.7116470140008815 1105 60-61 0.6452656599566353 1105 62-63 0.8145007402989535 1105 64-65 0.9151169470437992 1105 66-67 1.0473852890841542 1105 68-69 0.9664173382003511 1105 70-71 0.7333312740861082 1105 72-73 0.7527503788603447 1105 74-75 0.7971808616055469 1105 76-77 0.6483981352939701 1105 78-79 0.6686776301544519 1105 80-81 0.8140055620628353 1105 82-83 0.8001872420349976 1105 84-85 0.966461488292655 1105 86-87 0.7572085697446234 1105 88-89 0.9547777788982899 1105 90-91 1.0223686011000517 1105 92-93 1.0387511475459483 1105 94-95 0.8706744627789291 1105 96-97 0.7226329401218088 1105 98-99 0.635234486266981 1105 100-101 0.6476302464967247 1106 1 0.14616571136424739 1106 2 0.08725719384213093 1106 3 0.20735815069355468 1106 4 0.21535870705745452 1106 5 0.20581395074422915 1106 6 0.21113586016321761 1106 7 0.3174203303571943 1106 8 0.3143039144258566 1106 9 0.3186892699751809 1106 10-11 0.25619201883150566 1106 12-13 0.36162154918407907 1106 14-15 0.21123367825687467 1106 16-17 0.2909846151222766 1106 18-19 0.2813873364691233 1106 20-21 0.37496124929275965 1106 22-23 0.46207595938047064 1106 24-25 0.31247898294358123 1106 26-27 0.336021245836406 1106 28-29 0.262532294690736 1106 30-31 0.23118512131350855 1106 32-33 0.339155929675087 1106 34-35 0.19756167749118703 1106 36-37 0.16135214030656186 1106 38-39 0.4500563548556542 1106 40-41 0.3719729566308274 1106 42-43 0.34112342578172417 1106 44-45 0.34693638166238117 1106 46-47 0.35521816721836075 1106 48-49 0.37367625754649936 1106 50-51 0.26122515894046927 1106 52-53 0.3406767287666739 1106 54-55 0.5812242262946015 1106 56-57 0.3798538953336177 1106 58-59 0.5928074296721633 1106 60-61 0.654065827180716 1106 62-63 0.5971392973940297 1106 64-65 0.46914376261952384 1106 66-67 0.5250175752762161 1106 68-69 0.6237483224335065 1106 70-71 0.5754926453235676 1106 72-73 0.5713047864093177 1106 74-75 0.5689513465553802 1106 76-77 0.43271191933601827 1106 78-79 0.7553400166809681 1106 80-81 0.5792095157178743 1106 82-83 0.6255530447105819 1106 84-85 0.5817221786892155 1106 86-87 0.6351588803036314 1106 88-89 0.7309423751715798 1106 90-91 0.7790754811764948 1106 92-93 0.9131300295335265 1106 94-95 0.8497042644989499 1106 96-97 0.9488871207238141 1106 98-99 0.8836159960615326 1106 100-101 0.8217478408588832 1107 1 0.09120473778984461 1107 2 0.23419937087836473 1107 3 0.03936055956332396 1107 4 0.1310744971943194 1107 5 0.16873856616920335 1107 6 0.1226401639103969 1107 7 0.06010129210248749 1107 8 0.18063966791400077 1107 9 0.0975431869557184 1107 10-11 0.19690297172289917 1107 12-13 0.2975330353271346 1107 14-15 0.33727346182628537 1107 16-17 0.3063293890923049 1107 18-19 0.3122754188639476 1107 20-21 0.2554466464165941 1107 22-23 0.25171665349549244 1107 24-25 0.2477769292386114 1107 26-27 0.3293642319874195 1107 28-29 0.3075058977917777 1107 30-31 0.4247891729858324 1107 32-33 0.32996247281468527 1107 34-35 0.2536987238859112 1107 36-37 0.403305441325827 1107 38-39 0.5562824895232197 1107 40-41 0.26106472708060124 1107 42-43 0.45559574676047987 1107 44-45 0.3055732806416387 1107 46-47 0.500598749136195 1107 48-49 0.5197369685083544 1107 50-51 0.5663046814622987 1107 52-53 0.4475572087828539 1107 54-55 0.43161085841261126 1107 56-57 0.3880540073021237 1107 58-59 0.5830041323635129 1107 60-61 0.41299320751465984 1107 62-63 0.28864388364083027 1107 64-65 0.33142508605401133 1107 66-67 0.33117777094532386 1107 68-69 0.4270707421638207 1107 70-71 0.2689169958490929 1107 72-73 0.44691538355297666 1107 74-75 0.45938700805177746 1107 76-77 0.427880727432008 1107 78-79 0.448578836702481 1107 80-81 0.42798836627176584 1107 82-83 0.4058083615888357 1107 84-85 0.5076762258662022 1107 86-87 0.5856405085238165 1107 88-89 0.6264969970335059 1107 90-91 0.6207392649270709 1107 92-93 0.5210391668940346 1107 94-95 0.5018905088750181 1107 96-97 0.7036476316714619 1107 98-99 0.6440597939953854 1107 100-101 0.699947121022948 1108 1 0.29067333506958803 1108 2 0.2724107746649196 1108 3 0.3025195791386359 1108 4 0.15462222852938368 1108 5 0.27038415888637957 1108 6 0.25993735462753165 1108 7 0.3295045923882256 1108 8 0.24378177378916632 1108 9 0.30877094233765234 1108 10-11 0.3386910255948763 1108 12-13 0.30951453045227595 1108 14-15 0.43693307361091627 1108 16-17 0.49800320654660624 1108 18-19 0.5519754121076588 1108 20-21 0.3333091867532474 1108 22-23 0.4871682286067909 1108 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-2.6998889956540317 2316 74-75 -2.7304050941054108 2316 76-77 -2.6662434730976337 2316 78-79 -2.7537752641734414 2316 80-81 -2.533418194735919 2316 82-83 -2.360596071873317 2316 84-85 -2.4462643275581613 2316 86-87 -2.4959382834022286 2316 88-89 -2.584664723044213 2316 90-91 -2.825562634331188 2316 92-93 -2.828439994645194 2316 94-95 -2.8553720601425923 2316 96-97 -3.064119196630326 2316 98-99 -3.382529693997199 2316 100-101 -3.0535085452670714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7778.0 3 2374.0 4 2504.0 5 2631.0 6 3220.0 7 3953.0 8 4768.0 9 6024.0 10 7318.0 11 9044.0 12 11337.0 13 15179.0 14 13734.0 15 14266.0 16 17710.0 17 22543.0 18 19505.0 19 15263.0 20 15785.0 21 17073.0 22 18526.0 23 20767.0 24 23080.0 25 26451.0 26 30596.0 27 35357.0 28 40485.0 29 48257.0 30 57081.0 31 68894.0 32 84968.0 33 108060.0 34 143346.0 35 201438.0 36 295664.0 37 402542.0 38 305134.0 39 40592.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51851379496971 18.993329644842895 14.731128988798645 22.757027571388754 2 15.509254761091787 20.69706584792822 39.5127821469121 24.280897244067894 3 19.492690854035484 24.708855745703172 30.29362444056993 25.504828959691423 4 13.01279627805216 16.728126869777636 36.95164179491249 33.30743505725771 5 13.614250409574321 37.46137968696489 34.1621544072048 14.762215496255982 6 31.649428461216118 35.68262276035148 17.32104319125574 15.346905587176662 7 27.781873752898917 33.08318077496261 21.200787783982896 17.93415768815558 8 26.25476410730805 33.7313598519889 20.018501387604072 19.99537465309898 9 26.346624580697565 16.92765333101441 20.773348184302503 35.952373903985524 10-11 25.11650173336078 26.111374500748152 27.10879122658113 21.66333253930994 12-13 26.3776298039865 23.879016817378382 27.45525653214506 22.288096846490053 14-15 23.24347912960707 25.067019478663383 26.350102541189923 25.339398850539624 16-17 21.99455788731691 28.19827032767383 27.049626554327695 22.757545230681565 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26.31653632987193 28.26678074619905 23.213280418266848 44-45 22.583343204680645 26.16202016542055 28.317759831281254 22.936876798617547 46-47 23.241898180943217 26.4979313170313 27.924897541316756 22.33527296070873 48-49 22.79862445823641 26.514255078256937 27.95955420330496 22.727566260201694 50-51 22.320160758880032 26.816754435050115 28.354820733918828 22.508264072151025 52-53 22.772647958984575 26.991562588847863 27.416011476588764 22.819777975578802 54-55 22.483051018704096 26.77236442150489 27.555400505548704 23.189184054242315 56-57 22.79625779143002 26.833775550154726 27.327908146465184 23.042058511950074 58-59 23.107814846055245 26.48526374028725 27.475141609302046 22.93177980435546 60-61 22.607512033456956 26.7262498781569 28.183725474723936 22.48251261366221 62-63 22.292770851811415 26.737195168258832 28.27055372352075 22.699480256409004 64-65 22.191850892385915 27.04122592490727 27.78078471236257 22.986138470344244 66-67 22.507974342712043 26.78793707877698 27.35093845178107 23.353150126729904 68-69 22.222149937941364 26.75740254284741 27.82029900599304 23.20014851321819 70-71 22.870678951535854 26.514458017196834 27.4677001743475 23.147162856919813 72-73 22.538238874485426 26.30544172172507 27.926179922500072 23.230139481289434 74-75 22.174233237154024 27.365732286976762 27.26084108111122 23.199193394757994 76-77 22.411544988040937 26.655652903411013 27.314272229940993 23.618529878607053 78-79 22.407203357851532 26.614201204042892 27.64793646554777 23.330658972557806 80-81 22.71375908423566 26.502675515537778 27.396108670379547 23.38745672984701 82-83 22.50200198433343 26.29659839172377 27.888988807467634 23.31241081647517 84-85 22.29706174922898 26.62715585565687 27.633297310956262 23.442485084157887 86-87 22.299941356465595 26.656099217892663 27.554796866925628 23.48916255871611 88-89 22.251423331487587 26.457544400138243 27.33567582231769 23.95535644605648 90-91 22.368696141816717 26.087454446453563 27.554390144637736 23.989459267091988 92-93 22.57585130975825 26.33048620618119 27.4168889690104 23.676773515050158 94-95 22.47257863517202 26.01830538149222 27.643282032791333 23.86583395054443 96-97 21.988372970932428 25.998594534947877 28.00378662485118 24.00924586926852 98-99 22.125610665804317 26.220094537768762 27.46016225258873 24.19413254383819 100-101 22.38215596528448 25.132264317737242 28.114616223277128 24.370963493701154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2175.0 1 1345.5 2 555.0 3 614.0 4 557.0 5 625.5 6 995.5 7 1795.5 8 2870.5 9 3391.0 10 3163.0 11 2949.0 12 3072.5 13 3232.0 14 3426.0 15 3707.5 16 3962.5 17 4125.0 18 4266.0 19 4442.0 20 4750.0 21 5204.0 22 6112.0 23 7486.5 24 8792.5 25 10344.5 26 12559.0 27 15103.5 28 17389.5 29 20040.5 30 23024.0 31 26550.5 32 30763.5 33 34167.5 34 36498.0 35 39756.5 36 44937.0 37 49026.0 38 51848.5 39 53996.5 40 55366.0 41 57744.0 42 58312.0 43 58838.0 44 62584.0 45 66463.5 46 70207.0 47 79635.5 48 96099.0 49 97932.5 50 85300.0 51 79069.5 52 79090.0 53 80468.0 54 79776.5 55 74904.0 56 68428.0 57 62982.0 58 59048.5 59 53400.5 60 44225.5 61 36074.5 62 28865.0 63 21640.0 64 15557.0 65 11267.0 66 8325.0 67 6346.5 68 4843.5 69 3693.0 70 2785.0 71 1765.5 72 1188.0 73 801.5 74 504.5 75 366.5 76 268.0 77 161.0 78 87.5 79 62.0 80 45.5 81 32.5 82 26.0 83 21.0 84 19.0 85 16.0 86 14.0 87 11.0 88 12.0 89 12.0 90 8.0 91 5.0 92 2.0 93 3.0 94 6.0 95 3.5 96 0.5 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32321753066799064 2 0.08667518163651064 3 0.09804696546722083 4 0.10308568269302332 5 0.1141338791789572 6 0.055056075375511555 7 0.057090052971248334 8 0.05773722766080096 9 0.061250461689800854 10-11 0.05843062911389304 12-13 0.056835805771781246 14-15 0.055056075375511555 16-17 0.056442878281695724 18-19 0.06464812880995206 20-21 0.06591936480728756 22-23 0.08186759822840552 24-25 0.09007284875666186 26-27 0.08526526534855673 28-29 0.08327751451635941 30-31 0.0773836021650767 32-33 0.06880853752850458 34-35 0.31461935264964874 36-37 0.49266173242192646 38-39 0.46591954971434174 40-41 0.4484458330964212 42-43 0.4791404040866308 44-45 0.48787726239559104 46-47 0.4821451437166965 48-49 0.49869432506382766 50-51 0.49501929736243955 52-53 0.49430278252757776 54-55 0.48716074756072925 56-57 0.46605823000496016 58-59 0.4484227197146515 60-61 0.40859836292539603 62-63 0.4789323836507032 64-65 0.5000349012064723 66-67 0.5100661088945379 68-69 0.5201435433461428 70-71 0.5188954207305769 72-73 0.5079859045352616 74-75 0.5093727074414457 76-77 0.48482629600198585 78-79 0.4662662504408877 80-81 0.47179034868385467 82-83 0.48006493935742023 84-85 0.4817059894630715 86-87 0.48128994859121627 88-89 0.4878310356320516 90-91 0.5009594364772617 92-93 0.5067608953014655 94-95 0.5128859414704456 96-97 0.5055589994494393 98-99 0.5079165643899524 100-101 0.48778480886851217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2163249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.27715827460212 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48847548901261 31.210293857624233 2 14.11750925553313 12.501663835008014 3 5.671445124219443 7.533464202323543 4 2.822997652113827 4.999772554058963 5 1.653430704835036 3.660460650703392 6 1.0979488375456898 2.916843267445555 7 0.7675894933390768 2.379067704054717 8 0.5267558473008136 1.8658601618408217 9 0.40908215804674575 1.6301695913236405 >10 2.2281366694950755 17.721121781532364 >50 0.14860586043265778 4.460706430024772 >100 0.06309861156660063 4.99300946864996 >500 0.0024097621460130248 0.70141956066984 >1k 0.002095445344359145 1.9579440461396285 >5k 4.19089068871829E-4 1.4682028886005096 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9995 0.462036501577026 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8660 0.40032377225183047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7161 0.3310298537061614 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5833 0.26964071172574217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4512 0.20857515709009922 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4292 0.1984052691114153 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3647 0.1685890066284556 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2846 0.1315613690333383 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2534 0.1171386188090229 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2497 0.11542822855806244 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2431 0.11237726216445725 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2186 0.10105170509728653 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.622676353947234E-5 2 0.0 0.0 0.0 0.0 4.622676353947234E-5 3 0.0 0.0 0.0 4.622676353947234E-5 4.622676353947234E-5 4 0.0 0.0 0.0 2.311338176973617E-4 4.622676353947234E-5 5 1.3868029061841702E-4 0.0 0.0 2.311338176973617E-4 4.622676353947234E-5 6 1.3868029061841702E-4 0.0 0.0 2.311338176973617E-4 4.622676353947234E-5 7 1.3868029061841702E-4 0.0 0.0 2.311338176973617E-4 4.622676353947234E-5 8 1.3868029061841702E-4 0.0 0.0 3.2358734477630637E-4 4.622676353947234E-5 9 1.3868029061841702E-4 0.0 0.0 6.009479260131404E-4 9.245352707894468E-5 10-11 1.3868029061841702E-4 0.0 0.0 6.471746895526127E-4 9.245352707894468E-5 12-13 1.6179367238815319E-4 0.0 0.0 7.396282166315574E-4 9.245352707894468E-5 14-15 2.7736058123683404E-4 0.0 0.0 8.08968361940766E-4 9.245352707894468E-5 16-17 3.2358734477630637E-4 0.0 0.0 8.783085072499745E-4 1.8490705415788935E-4 18-19 3.698141083157787E-4 0.0 0.0 9.476486525591831E-4 1.8490705415788935E-4 20-21 3.698141083157787E-4 0.0 0.0 0.0010401021796381277 1.8490705415788935E-4 22-23 3.698141083157787E-4 0.0 0.0 0.0012712359973354893 2.311338176973617E-4 24-25 4.1604087185525103E-4 0.0 0.0 0.0015717099603420595 3.2358734477630637E-4 26-27 4.1604087185525103E-4 0.0 0.0 0.0023113381769736168 3.4670072654604254E-4 28-29 4.853810171644596E-4 0.0 0.0 0.0061712729325195574 3.698141083157787E-4 30-31 5.084943989341958E-4 0.0 0.0 0.015740212985190332 4.391542536249872E-4 32-33 5.084943989341958E-4 0.0 0.0 0.03358374371142665 5.084943989341958E-4 34-35 5.547211624736681E-4 0.0 0.0 0.0585924227862812 5.547211624736681E-4 36-37 6.471746895526127E-4 0.0 0.0 0.10218426080400361 5.547211624736681E-4 38-39 9.938754160986553E-4 0.0 0.0 0.16304179500371896 6.009479260131404E-4 40-41 0.0010169887978683915 0.0 0.0 0.23568715390599973 6.471746895526127E-4 42-43 0.0011556690884868084 0.0 0.0 0.3081476057541226 6.471746895526127E-4 44-45 0.0013868029061841701 0.0 0.0 0.4003931123971397 6.702880713223489E-4 46-47 0.0015254831968025873 0.0 0.0 0.5122156533991232 6.934014530920851E-4 48-49 0.0015254831968025873 0.0 0.0 0.6303481476242448 6.934014530920851E-4 50-51 0.0015254831968025873 0.0 0.0 0.7586043030645109 6.934014530920851E-4 52-53 0.0015254831968025873 0.0 0.0 0.8872302726130926 6.934014530920851E-4 54-55 0.001617936723881532 0.0 0.0 1.0327752376171213 6.934014530920851E-4 56-57 0.001756617014499949 0.0 0.0 1.21160347236957 7.165148348618213E-4 58-59 0.001756617014499949 0.0 0.0 1.403375200913071 7.627415984012935E-4 60-61 0.0017797303962696852 0.0 0.0 1.6188843725340911 7.858549801710297E-4 62-63 0.0018028437780394212 0.0 0.0 1.833353441975473 7.858549801710297E-4 64-65 0.0018028437780394212 0.0 0.0 2.068647668391387 7.858549801710297E-4 66-67 0.0018490705415788937 0.0 0.0 2.336300629284932 8.08968361940766E-4 68-69 0.0018490705415788937 0.0 0.0 2.6476841084868177 8.320817437105021E-4 70-71 0.0018721839233486297 0.0 0.0 2.983637112509933 8.320817437105021E-4 72-73 0.0019646374504275746 0.0 0.0 3.3192203024247324 8.783085072499745E-4 74-75 0.002033977595736783 0.0 0.0 3.681060294029952 8.783085072499745E-4 76-77 0.002033977595736783 0.0 0.0 4.068232552054803 9.014218890197106E-4 78-79 0.002033977595736783 0.0 0.0 4.52573883080496 9.245352707894468E-4 80-81 0.002311338176973617 0.0 0.0 5.022792105763137 9.707620343289192E-4 82-83 0.002357564940513089 0.0 0.0 5.516886867854787 0.0010169887978683915 84-85 0.002426905085822298 0.0 0.0 6.02653693587747 0.0010401021796381275 86-87 0.0024962452311315065 0.0 0.0 6.558815004652724 0.0011094423249473362 88-89 0.0024962452311315065 0.0 0.0 7.146819436874812 0.0011094423249473362 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8915 0.0 22.477154 2 GAGTACT 7255 0.0 22.385561 12-13 GTACATG 9100 0.0 22.377592 1 ACATGGG 9165 0.0 20.933643 3 AGTACTT 7805 0.0 20.53511 12-13 GTACTTT 8035 0.0 20.065386 14-15 AGAGTAC 13140 0.0 19.728998 10-11 CATGGGG 4715 0.0 17.9843 4 CATGGGA 4770 0.0 17.478998 4 TTTGCGT 570 0.0 17.452864 3 TACTTTT 8935 0.0 17.434828 14-15 GTATCAA 22495 0.0 17.366459 1 CATACCG 165 0.0014910686 17.226202 5 ACTTTTT 9590 0.0 16.987383 16-17 GGTATCA 18575 0.0 16.738245 1 CCGTTGC 255 5.680118E-6 16.71143 9 TATTCCG 290 1.0597068E-6 16.335192 5 CAACGCA 24960 0.0 15.524978 5 TATCAAC 25260 0.0 15.415609 2 AACGCAG 25210 0.0 15.401118 6 >>END_MODULE