FastQCFastQC Report
Fri 27 May 2016
SRR938947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163249
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106430.4919914443506041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87170.4029586977735804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76540.3538196481311213No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54330.2511500063099532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48380.22364508200396715No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40310.186340083827613No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33900.15670872839881123No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30570.14131521614016693No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28670.1325321310676672No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26630.12310187130561484No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25720.11889523582352286No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25000.11556690884868086No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24820.11473482710497034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT81550.023.0461512-13
CCTATAC5100.022.3330573
GTACTTT86250.021.35009814-15
GTACATG87950.020.364241
AGTACTT86150.019.80481512-13
TACATGG87150.019.603892
AGAGTAC137750.019.15624610-11
CGTACAC1250.00606391218.9830993
ACTTTTT99700.018.56464416-17
CTATGCG1559.6859783E-418.3707399
ACATGGG88250.017.907573
CCCTATA6400.017.7966562
TAACACG1600.001198608117.7966565
TTTGCGT4800.017.7966563
GGTATCA195500.017.5429211
TACTTTT96900.017.36279714-15
CTATACT7450.017.1994514
GTAAGCG2900.017.18357394-95
GTATCAA233000.017.0500761
GTGTACG2253.4554698E-516.93651
Produced by FastQC (version 0.11.5)