##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938947_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2163249 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.151738889050684 33.0 31.0 34.0 30.0 34.0 2 32.387893857803704 34.0 31.0 34.0 31.0 34.0 3 32.47203535053061 34.0 31.0 34.0 30.0 34.0 4 36.03960246832427 37.0 35.0 37.0 35.0 37.0 5 35.959213201993855 37.0 35.0 37.0 35.0 37.0 6 36.00166924843141 37.0 35.0 37.0 35.0 37.0 7 35.93550210817155 37.0 35.0 37.0 35.0 37.0 8 35.90531325797446 37.0 35.0 37.0 35.0 37.0 9 37.569153620318325 39.0 37.0 39.0 35.0 39.0 10-11 37.479099031133266 39.0 37.0 39.0 34.5 39.0 12-13 37.39078603526455 39.0 37.0 39.0 34.0 39.0 14-15 38.677379256849306 40.0 38.0 41.0 34.0 41.0 16-17 38.77222247646942 40.0 38.0 41.0 34.5 41.0 18-19 38.814490379979375 40.0 38.0 41.0 34.0 41.0 20-21 38.78555057693312 40.0 38.0 41.0 34.0 41.0 22-23 38.7044311588726 40.0 38.0 41.0 34.0 41.0 24-25 38.62222356279837 40.0 38.0 41.0 34.0 41.0 26-27 38.45549980607873 40.0 38.0 41.0 34.0 41.0 28-29 38.29605745801801 40.0 38.0 41.0 34.0 41.0 30-31 38.08696016963373 40.0 37.5 41.0 33.0 41.0 32-33 37.97764681735667 40.0 37.0 41.0 33.0 41.0 34-35 37.87112093892104 40.0 37.0 41.0 33.0 41.0 36-37 37.73500161100271 40.0 37.0 41.0 33.0 41.0 38-39 37.58500315959928 40.0 37.0 41.0 32.5 41.0 40-41 37.409062941898966 40.0 37.0 41.0 32.0 41.0 42-43 37.20395086279943 40.0 36.0 41.0 31.0 41.0 44-45 36.93311056655983 40.0 36.0 41.0 31.0 41.0 46-47 36.5886289557975 39.0 36.0 41.0 30.0 41.0 48-49 36.21499905928536 39.0 35.0 41.0 29.5 41.0 50-51 35.9317531176485 39.0 35.0 40.0 29.0 41.0 52-53 35.66239785618761 39.0 35.0 40.0 28.0 41.0 54-55 35.34959059266871 38.0 34.5 40.0 27.5 41.0 56-57 35.185102131100024 38.0 34.0 40.0 27.5 41.0 58-59 34.884505667170075 38.0 34.0 40.0 26.5 41.0 60-61 34.49005870336702 37.5 34.0 40.0 26.0 41.0 62-63 34.05680414043876 37.0 33.0 40.0 26.0 41.0 64-65 33.63472836460343 36.0 33.0 39.0 25.0 40.5 66-67 33.15383434824193 36.0 32.5 39.0 23.5 40.0 68-69 32.6490108166004 35.0 32.0 38.5 22.0 40.0 70-71 32.10554240404133 35.0 31.0 37.5 21.5 40.0 72-73 31.581299702438322 35.0 31.0 37.0 19.5 39.0 74-75 31.05619625849821 34.5 30.5 36.5 18.0 39.0 76-77 29.193721804563413 32.5 28.0 34.5 13.0 36.5 78-79 30.15305912541737 34.0 29.5 35.0 14.5 37.0 80-81 30.14052381394837 34.0 30.0 35.0 12.0 37.0 82-83 29.730171838748106 34.0 29.5 35.0 7.5 36.5 84-85 29.277361736905924 34.0 29.0 35.0 4.5 36.0 86-87 28.59391822208169 33.5 28.5 35.0 2.0 35.5 88-89 27.888432399598937 33.0 27.0 35.0 2.0 35.0 90-91 27.29445916766863 33.0 26.0 34.5 2.0 35.0 92-93 26.873480121798277 32.0 25.0 34.0 2.0 35.0 94-95 26.290849550837653 32.0 25.0 34.0 2.0 35.0 96-97 25.405556641884502 32.0 22.0 34.0 2.0 35.0 98-99 24.15853676576298 31.5 10.0 34.0 2.0 35.0 100-101 18.61633866466597 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13269862883353056 1101 2 0.17121635123606893 1101 3 0.23157951224889217 1101 4 0.13208985314715704 1101 5 0.11030547483316155 1101 6 0.1253242196801736 1101 7 0.19043499769063033 1101 8 0.12144862978887261 1101 9 0.15383203836806558 1101 10-11 0.2800982577943216 1101 12-13 0.2994403493056126 1101 14-15 0.3187515445155711 1101 16-17 0.2901731142096722 1101 18-19 0.3487425288959827 1101 20-21 0.34677428303762525 1101 22-23 0.2794797151158619 1101 24-25 0.30609224296859594 1101 26-27 0.31826486667338116 1101 28-29 0.385595965187882 1101 30-31 0.2492964200498804 1101 32-33 0.40821273164000615 1101 34-35 0.42185143759491694 1101 36-37 0.48975659661819293 1101 38-39 0.43952021759002946 1101 40-41 0.5285804561618335 1101 42-43 0.6094137982419667 1101 44-45 0.553540088818643 1101 46-47 0.453997600270192 1101 48-49 0.48222568949610434 1101 50-51 0.40061689716889504 1101 52-53 0.33312989566799445 1101 54-55 0.46090441441648977 1101 56-57 0.5085235377394355 1101 58-59 0.4590479400065277 1101 60-61 0.4515388910154243 1101 62-63 0.516173989545095 1101 64-65 0.5601893873139048 1101 66-67 0.4835761750588077 1101 68-69 0.5543245653648654 1101 70-71 0.6923004936440904 1101 72-73 0.4949742273191511 1101 74-75 0.5370315074452705 1101 76-77 0.3539417906291753 1101 78-79 0.6802008743449335 1101 80-81 0.5551293297470679 1101 82-83 0.6136256804506033 1101 84-85 0.8492206877829744 1101 86-87 0.9346125766713875 1101 88-89 0.8706128350929561 1101 90-91 0.8965110621101395 1101 92-93 0.7382280442319349 1101 94-95 0.5940751775478645 1101 96-97 0.781092070914962 1101 98-99 0.8239387943379342 1101 100-101 1.3742655724517618 1102 1 0.18823253153189512 1102 2 0.17856184128227426 1102 3 0.19923443213541958 1102 4 0.1811263850910052 1102 5 0.19465853160695445 1102 6 0.14652543610826285 1102 7 0.19101420110933987 1102 8 0.17595732225481697 1102 9 0.08685205696009035 1102 10-11 0.2769173059179053 1102 12-13 0.2756327174933162 1102 14-15 0.32589320339351957 1102 16-17 0.3114059900580841 1102 18-19 0.32179720887725694 1102 20-21 0.2865121531280508 1102 22-23 0.3086916478162536 1102 24-25 0.31643995421953974 1102 26-27 0.25682856328279513 1102 28-29 0.3346036126406844 1102 30-31 0.36448676127788815 1102 32-33 0.30170262143747095 1102 34-35 0.3361427017391776 1102 36-37 0.40803001039924425 1102 38-39 0.4033004532824265 1102 40-41 0.3179018976619119 1102 42-43 0.4339599396397418 1102 44-45 0.26882376857667367 1102 46-47 0.42369360676856616 1102 48-49 0.42812957855948497 1102 50-51 0.25916714798466245 1102 52-53 0.30159837436347203 1102 54-55 0.4009755282857128 1102 56-57 0.4161949558418101 1102 58-59 0.33807351097975413 1102 60-61 0.39235810058944054 1102 62-63 0.5599689323626293 1102 64-65 0.6095377204012848 1102 66-67 0.3172226970002967 1102 68-69 0.3082365219044263 1102 70-71 0.4244509503091578 1102 72-73 0.5619166210488196 1102 74-75 0.6113752682060074 1102 76-77 0.26895617611478073 1102 78-79 0.4476388452157458 1102 80-81 0.5890501912869262 1102 82-83 0.6413911182020051 1102 84-85 0.8650392982245272 1102 86-87 1.0330612650758404 1102 88-89 0.8630527925608575 1102 90-91 0.7946041811684772 1102 92-93 0.8411014834918866 1102 94-95 0.9970299726887077 1102 96-97 1.0009797367327486 1102 98-99 1.201835599763676 1102 100-101 1.135225470939801 1103 1 -0.006879555812574267 1103 2 0.0234336672760449 1103 3 0.015040152910714255 1103 4 -0.007080299983059035 1103 5 0.10422936960240747 1103 6 0.034198593581116654 1103 7 0.05154799899049323 1103 8 0.09528376461749133 1103 9 0.07485563166259368 1103 10-11 0.06758761769332011 1103 12-13 0.02301644518927759 1103 14-15 0.06620010126298581 1103 16-17 -0.023541181474833195 1103 18-19 0.04694277840089711 1103 20-21 0.05387607531045546 1103 22-23 0.024338879436406557 1103 24-25 0.12179497568302367 1103 26-27 0.008542275760497375 1103 28-29 0.08606518896836235 1103 30-31 0.0641813449429165 1103 32-33 0.06498606726648859 1103 34-35 0.0024613412700702497 1103 36-37 0.054292376645086904 1103 38-39 0.0598272008612355 1103 40-41 0.0508263992143867 1103 42-43 0.11500983707100687 1103 44-45 0.08559511449821144 1103 46-47 0.024324835563788838 1103 48-49 -0.005353651696253792 1103 50-51 -0.04064550797374267 1103 52-53 0.010805810965933915 1103 54-55 0.11215125354314637 1103 56-57 0.18152890047496584 1103 58-59 0.08473286340402808 1103 60-61 -0.026986986084082787 1103 62-63 0.14743152173958407 1103 64-65 0.1464100359020577 1103 66-67 0.17034331668213554 1103 68-69 0.1462956666636117 1103 70-71 0.25591764193500666 1103 72-73 0.10535260987424167 1103 74-75 0.03656303143176487 1103 76-77 -0.13475396666560258 1103 78-79 0.07264319346944603 1103 80-81 0.17581480773841562 1103 82-83 0.26885201082153287 1103 84-85 0.291605989675201 1103 86-87 0.3454852332008258 1103 88-89 0.16054003997736643 1103 90-91 0.05562490838669376 1103 92-93 0.22439460434180702 1103 94-95 0.38057823279911673 1103 96-97 0.22050848863753814 1103 98-99 0.38969617635587994 1103 100-101 0.7218266681297933 1104 1 0.05325864957503512 1104 2 0.09530242849819359 1104 3 0.12366316966830482 1104 4 0.11126192124844181 1104 5 0.050863767115593816 1104 6 0.11205829625091468 1104 7 0.12095958502101922 1104 8 0.13014277895489101 1104 9 0.11350708806126875 1104 10-11 0.1630858993119091 1104 12-13 0.20293183664465175 1104 14-15 0.26267601085383063 1104 16-17 0.1822826985152659 1104 18-19 0.16973466440717289 1104 20-21 0.295045823958894 1104 22-23 0.16953243311775879 1104 24-25 0.25629965805804034 1104 26-27 0.21155844544790625 1104 28-29 0.23222546998434979 1104 30-31 0.10859985104115566 1104 32-33 0.1884002694500424 1104 34-35 0.2522463904944061 1104 36-37 0.21649153741847016 1104 38-39 0.2997051627114544 1104 40-41 0.2837615122503294 1104 42-43 0.1618422832475801 1104 44-45 0.23719750822924368 1104 46-47 0.3192987842646531 1104 48-49 0.14981276062219706 1104 50-51 0.13048899119707613 1104 52-53 0.06834292824944299 1104 54-55 0.34524889631087774 1104 56-57 0.10067201237429657 1104 58-59 0.27349785790472225 1104 60-61 0.2571563830213037 1104 62-63 0.2487035894068228 1104 64-65 0.3028045459000239 1104 66-67 0.2705868050821394 1104 68-69 0.24356058645206247 1104 70-71 0.24196776527143982 1104 72-73 0.33786161821016947 1104 74-75 0.4351388447540607 1104 76-77 0.3146452734742198 1104 78-79 0.623213578519163 1104 80-81 0.4808358373954995 1104 82-83 0.5757594631832958 1104 84-85 0.5084506592633993 1104 86-87 0.6406708257423439 1104 88-89 0.5029566256037157 1104 90-91 0.7695168524480529 1104 92-93 0.9305104744453985 1104 94-95 1.307838032962259 1104 96-97 1.1429709059335416 1104 98-99 1.0130192541080447 1104 100-101 1.1167251697207945 1105 1 0.008215546126763229 1105 2 0.0188956456258893 1105 3 0.042692631585694585 1105 4 0.038698923900959414 1105 5 0.014403460181149796 1105 6 0.02389516627744115 1105 7 0.0749407331832046 1105 8 0.06025598471465088 1105 9 0.08950415134280831 1105 10-11 0.14540756471546956 1105 12-13 0.22996859299175298 1105 14-15 0.11008625147528761 1105 16-17 0.029919113824099952 1105 18-19 0.18527677316048852 1105 20-21 0.22159233778989318 1105 22-23 0.14584093065470682 1105 24-25 0.008335230244476577 1105 26-27 0.04096825977151752 1105 28-29 0.220292682943807 1105 30-31 0.25283772523592063 1105 32-33 0.24174789165542165 1105 34-35 0.181874090380731 1105 36-37 0.12128975644083084 1105 38-39 0.19353686979746954 1105 40-41 0.22630011588806553 1105 42-43 0.2541120824139327 1105 44-45 0.32836959045736336 1105 46-47 0.3446942939274109 1105 48-49 0.3563280126115913 1105 50-51 0.39040988926452513 1105 52-53 0.20395419198570863 1105 54-55 0.284203045609857 1105 56-57 0.36226968194610976 1105 58-59 0.25823521106879355 1105 60-61 0.3496061746090291 1105 62-63 0.4737012210854843 1105 64-65 0.47372984904515647 1105 66-67 0.486236672456144 1105 68-69 0.602163513697235 1105 70-71 0.5920269733047832 1105 72-73 0.5159937231741658 1105 74-75 0.5646883900363626 1105 76-77 0.4677726417955519 1105 78-79 0.5961602439227249 1105 80-81 0.431958421707737 1105 82-83 0.517385078946841 1105 84-85 0.5548887039773085 1105 86-87 0.806458055753712 1105 88-89 0.8216557066950365 1105 90-91 0.7381614621486996 1105 92-93 0.9531433372722269 1105 94-95 0.8976779344400967 1105 96-97 1.015050765728919 1105 98-99 1.4313678236419634 1105 100-101 1.5037834777920338 1106 1 0.014334155777980584 1106 2 -0.003315295606789448 1106 3 0.036550132780149625 1106 4 -0.00913612148843157 1106 5 0.045581792718174086 1106 6 0.053750636893781234 1106 7 0.12063172697202162 1106 8 0.13158601338163578 1106 9 0.085314590903252 1106 10-11 0.10609049591470665 1106 12-13 0.10892224803235706 1106 14-15 0.05270599586133784 1106 16-17 -0.06909545856003518 1106 18-19 -0.07236626315102512 1106 20-21 -0.008182506930602074 1106 22-23 -0.07816020408012747 1106 24-25 0.06977066980491742 1106 26-27 -0.11757808518787272 1106 28-29 0.016480869756996697 1106 30-31 0.12300912991819501 1106 32-33 0.09229584913272504 1106 34-35 0.022885796066347552 1106 36-37 0.0973349069424998 1106 38-39 0.08141760319450952 1106 40-41 0.19673579434005006 1106 42-43 0.10621432799444364 1106 44-45 0.13677409356295556 1106 46-47 0.23893402159104227 1106 48-49 0.3904267942647266 1106 50-51 0.3654964874489437 1106 52-53 0.32154407492885184 1106 54-55 0.2653545028482682 1106 56-57 0.48294873115776227 1106 58-59 0.3655139377768677 1106 60-61 0.3519831454642599 1106 62-63 0.43208332810889516 1106 64-65 0.4910659097236092 1106 66-67 0.42082816791720745 1106 68-69 0.32079257485347057 1106 70-71 0.530527331642098 1106 72-73 0.6610382167240978 1106 74-75 0.6142135930941741 1106 76-77 0.38296166472914095 1106 78-79 0.5158359486527786 1106 80-81 0.5585769119131889 1106 82-83 0.44695985199987476 1106 84-85 0.6621510068917829 1106 86-87 0.8887143863796076 1106 88-89 0.8967199087972837 1106 90-91 1.1828774798266686 1106 92-93 1.2564952842383015 1106 94-95 1.2290623109331662 1106 96-97 1.148516484792463 1106 98-99 1.2385748828870646 1106 100-101 1.5307588122401974 1107 1 0.028109375314933516 1107 2 0.07355172980449964 1107 3 0.018808259857003407 1107 4 -0.014979603695330468 1107 5 0.003143623701340914 1107 6 -0.00848896179532943 1107 7 0.03437450389395735 1107 8 0.07018203063025652 1107 9 0.03957431522398025 1107 10-11 0.056939822052214595 1107 12-13 0.016802148924320193 1107 14-15 0.07485755331985189 1107 16-17 0.05449722810774915 1107 18-19 0.10833237634425785 1107 20-21 0.08567361960052722 1107 22-23 0.05857409583536821 1107 24-25 0.021467494800660347 1107 26-27 0.1325571671100647 1107 28-29 0.04958971304885296 1107 30-31 0.13103525799410676 1107 32-33 0.1284403301999859 1107 34-35 0.15350577286999112 1107 36-37 0.12051321599393816 1107 38-39 0.0047325209904869325 1107 40-41 -0.054473965438859295 1107 42-43 -0.024956971479149104 1107 44-45 0.07884866763311749 1107 46-47 -0.05391483685264831 1107 48-49 -0.09622924653001519 1107 50-51 -0.09514514990090817 1107 52-53 -0.1431238546157374 1107 54-55 -0.030021045643792377 1107 56-57 0.030000388006818923 1107 58-59 -0.05554838186337463 1107 60-61 -0.0017375426779082659 1107 62-63 0.17157081102159566 1107 64-65 0.09259795448500086 1107 66-67 0.021236491970277882 1107 68-69 0.0844081036333435 1107 70-71 0.1609488860940118 1107 72-73 0.06665554229123316 1107 74-75 -0.1280355478043056 1107 76-77 -0.02370763561122402 1107 78-79 0.11765408033033253 1107 80-81 0.10589761601882586 1107 82-83 0.01184777251793534 1107 84-85 0.0752475098828036 1107 86-87 0.2049090357919745 1107 88-89 0.2844423408140848 1107 90-91 0.29388906836463136 1107 92-93 0.45481091388847616 1107 94-95 0.688673945702412 1107 96-97 0.8200008162649617 1107 98-99 0.8823732427301465 1107 100-101 1.1713259145317387 1108 1 0.12007279392303616 1108 2 0.11930719538857204 1108 3 0.12449544556896797 1108 4 0.06233760769512742 1108 5 -0.03199665659727202 1108 6 0.042258573193734605 1108 7 0.05685938382737277 1108 8 0.17149072817882427 1108 9 0.19314358854615676 1108 10-11 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20.0 5 43.0 6 112.0 7 304.0 8 706.0 9 1312.0 10 2270.0 11 3459.0 12 5025.0 13 6762.0 14 8176.0 15 11100.0 16 14534.0 17 20122.0 18 19985.0 19 14370.0 20 13605.0 21 14697.0 22 15829.0 23 17550.0 24 19986.0 25 23941.0 26 28483.0 27 34054.0 28 41400.0 29 49477.0 30 60394.0 31 73521.0 32 90501.0 33 115224.0 34 154769.0 35 220735.0 36 337996.0 37 464337.0 38 266260.0 39 12186.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66228203293302 19.676090287813523 15.222586989592529 22.43904068966093 2 15.934411618819656 21.39439334075735 38.659303667770104 24.011891372652894 3 19.545692613286775 24.88474512180521 30.50582711467797 25.06373515023005 4 13.408951073131202 17.33841088104051 36.614786369946316 32.637851675881976 5 13.595244930195275 37.681237804801945 34.12136097139072 14.602156293612062 6 31.591555109929555 36.20910029312391 16.948673037639217 15.25067155930732 7 27.750111059799405 33.33411918831351 20.918719943936182 17.99704980795091 8 26.242933661358446 34.27293852903665 19.675959632941005 19.808168176663898 9 26.100000508494396 16.98791955988423 20.642653712078452 36.269426219542915 10-11 25.145232934350137 26.387554091091687 26.895863582971725 21.571349391586452 12-13 26.40475044712837 23.98875487750139 27.38101346631849 22.225481209051758 14-15 23.09981442098492 25.317054894083128 26.255998265556197 25.327132419375754 16-17 21.889615231529998 28.356871796697202 27.104165732478364 22.649347239294435 18-19 21.89467100521856 27.26344406379132 29.10517136093482 21.7367135700553 20-21 23.049819969869397 25.89713435670142 28.946737060782183 22.106308612647 22-23 22.62381723047139 25.847116998551716 29.169434494133593 22.359631276843302 24-25 22.89550250364267 26.06518069125685 28.98867813584018 22.050638669260298 26-27 22.387025808639184 25.87233967803514 29.477612026542488 22.26302248678319 28-29 22.179855393438295 26.87956864882406 29.001816249539463 21.938759708198177 30-31 22.5210089083596 26.108922273857516 28.796846780005446 22.573222037777434 32-33 22.377636601241928 26.127505432800387 28.973525470253307 22.521332495704378 34-35 22.974305296618446 26.165309457360543 28.763467394497944 22.096917851523067 36-37 22.81695174890538 25.79394133872403 29.142751192527438 22.24635571984315 38-39 22.459448282589605 26.434396150363646 28.927727779281597 22.17842778776515 40-41 22.53296100155123 26.372869430307873 28.86322539456236 22.230944173578543 42-43 22.228475998405095 26.364898209198444 28.337010476680284 23.069615315716177 44-45 22.665968629952708 26.116835628533526 28.398213765653818 22.81898197585995 46-47 23.26749711021271 26.540509143423364 27.982571364051257 22.209422382312678 48-49 22.8725837383502 26.58225858532906 27.933601503156346 22.611556173164388 50-51 22.40763135194806 26.87124924996556 28.260858510914677 22.460260887171707 52-53 22.846699570876954 27.069907347697836 27.379857797230002 22.70353528419521 54-55 22.55893796784374 26.823241337451215 27.44820406712311 23.169616627581938 56-57 22.823747982779604 26.843095732391415 27.352745800414098 22.98041048441488 58-59 23.139253274384476 26.556965021303782 27.422614598256033 22.88116710605571 60-61 22.610482044421413 26.841733374907285 28.14139704656984 22.406387534101466 62-63 22.344496247375726 26.852984637019034 28.199681077209227 22.602838038396012 64-65 22.21518800092824 27.196572354169728 27.667210297175433 22.9210293477266 66-67 22.51247981283228 26.88844063187135 27.24275475125087 23.3563248040455 68-69 22.239716973347445 26.8127349974355 27.708565388545658 23.2389826406714 70-71 22.84107574376025 26.579968311531623 27.359698786199914 23.219257158508217 72-73 22.512711204304267 26.411961822240528 27.729748170460265 23.34557880299494 74-75 22.13739611112729 27.485925106171322 27.0038724159817 23.37280636671969 76-77 22.358918575278473 26.677866827435704 27.23769750173335 23.72551709555247 78-79 22.38025950371388 26.6905359848463 27.52301890630291 23.406185605136912 80-81 22.70317908901232 26.630380139470994 27.19268340093623 23.473757370580454 82-83 22.458533437436003 26.43713229498777 27.640830990792093 23.463503276784134 84-85 22.1595745836522 26.702355326753253 27.444365422232476 23.693704667362073 86-87 22.16345092784564 26.743617374197065 27.25194640070291 23.84098529725438 88-89 22.091557252358506 26.576145231722943 27.032420249371537 24.29987726654702 90-91 22.18203932999191 26.160099254270385 27.29685022689309 24.361011188844614 92-93 22.321471083541468 26.462834375515715 27.122836991950532 24.09285754899228 94-95 22.173550059032205 26.13915513219608 27.329368267578147 24.35792654119357 96-97 21.64593627455739 26.214596655308753 27.66627882412057 24.47318824601329 98-99 21.72216420763398 26.479637804062317 27.118075635305967 24.680122352997735 100-101 21.91762774411814 25.268503432697383 27.898469911228084 24.91539891195639 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 975.0 1 684.5 2 441.0 3 532.0 4 504.0 5 486.0 6 785.5 7 1602.0 8 2520.0 9 2937.0 10 2714.5 11 2461.0 12 2530.0 13 2655.0 14 2942.0 15 3126.0 16 3234.0 17 3415.5 18 3645.5 19 3877.5 20 4262.5 21 4931.5 22 5935.5 23 7274.5 24 8726.0 25 10291.5 26 12400.5 27 14952.0 28 17240.5 29 20052.0 30 23130.5 31 26891.5 32 31238.0 33 34456.0 34 36937.0 35 40081.5 36 44864.0 37 49121.0 38 52142.5 39 54265.0 40 55644.0 41 57584.5 42 58208.0 43 59182.0 44 62862.5 45 66646.0 46 70219.5 47 80579.5 48 97801.5 49 98363.0 50 85645.5 51 80701.5 52 80941.0 53 82338.0 54 81319.5 55 75748.5 56 69406.5 57 63877.5 58 59291.5 59 53646.5 60 44584.0 61 35621.5 62 28149.5 63 21215.5 64 14933.0 65 10475.5 66 7608.5 67 5806.5 68 4386.0 69 3295.0 70 2413.0 71 1466.0 72 941.5 73 628.0 74 379.5 75 242.0 76 153.0 77 83.0 78 37.0 79 16.5 80 11.0 81 8.0 82 3.0 83 2.0 84 1.0 85 2.0 86 3.0 87 2.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3906623786720807 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.014218890197106E-4 16-17 0.0024037917040525617 18-19 3.698141083157787E-4 20-21 0.0 22-23 0.0 24-25 4.622676353947234E-5 26-27 2.311338176973617E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013567555098835133 36-37 0.02359876278690063 38-39 0.011071309867703626 40-41 0.005755232060664306 42-43 0.005154284134651166 44-45 0.0018028437780394212 46-47 0.0012712359973354895 48-49 0.0031203065389143825 50-51 6.934014530920851E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.320817437105022E-4 60-61 0.0016410501056512681 62-63 0.0013636895244144341 64-65 6.934014530920851E-4 66-67 0.0025655853764407145 68-69 0.0027967191941380767 70-71 6.934014530920851E-5 72-73 0.0 74-75 0.0 76-77 0.09108983755453025 78-79 0.003767481228466996 80-81 1.6179367238815319E-4 82-83 0.0 84-85 1.6179367238815319E-4 86-87 0.09023464242905001 88-89 0.4342773300715729 90-91 0.38735716507900847 92-93 0.0 94-95 0.0010632155614078637 96-97 0.0 98-99 0.0 100-101 3.929274900855149E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2163249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.1124462966997 #Duplication Level Percentage of deduplicated Percentage of total 1 68.39487106541931 28.80275334712171 2 14.875816259067227 12.529140266590815 3 6.104304657244459 7.7120160617070335 4 3.0710708532364164 5.173212255211132 5 1.799418956391835 3.788896708315728 6 1.1991273302820056 3.0298911179643504 7 0.8526276961023609 2.513436664423262 8 0.5896650049074461 1.9865788681766392 9 0.43753306582162743 1.6583028963699293 >10 2.4338280365821885 18.370104565747315 >50 0.1658631220258818 4.7695985013326325 >100 0.07015770543139811 5.295101557190245 >500 0.003187907252834625 0.8808689319782212 >1k 0.002088628889788197 1.988019519385782 >5k 3.2978350891392586E-4 1.0093791356344912 >10k+ 1.0992783630464197E-4 0.4926996028507562 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10643 0.4919914443506041 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8717 0.4029586977735804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7654 0.3538196481311213 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5433 0.2511500063099532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4838 0.22364508200396715 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4031 0.186340083827613 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3390 0.15670872839881123 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3057 0.14131521614016693 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2867 0.1325321310676672 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2663 0.12310187130561484 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2572 0.11889523582352286 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2500 0.11556690884868086 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2482 0.11473482710497034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3868029061841702E-4 0.0 5 0.0 0.0 0.0 1.3868029061841702E-4 0.0 6 0.0 0.0 0.0 1.3868029061841702E-4 4.622676353947234E-5 7 4.622676353947234E-5 0.0 0.0 1.3868029061841702E-4 4.622676353947234E-5 8 4.622676353947234E-5 0.0 0.0 1.3868029061841702E-4 4.622676353947234E-5 9 4.622676353947234E-5 0.0 0.0 4.622676353947234E-4 4.622676353947234E-5 10-11 4.622676353947234E-5 0.0 0.0 4.853810171644596E-4 4.622676353947234E-5 12-13 9.245352707894468E-5 0.0 0.0 5.547211624736681E-4 1.1556690884868084E-4 14-15 1.3868029061841702E-4 0.0 0.0 6.240613077828766E-4 2.542471994670979E-4 16-17 1.3868029061841702E-4 0.0 0.0 6.934014530920851E-4 2.7736058123683404E-4 18-19 1.8490705415788938E-4 0.0 0.0 7.165148348618213E-4 2.7736058123683404E-4 20-21 2.311338176973617E-4 0.0 0.0 8.08968361940766E-4 2.7736058123683404E-4 22-23 2.311338176973617E-4 0.0 0.0 0.0010401021796381277 3.2358734477630637E-4 24-25 4.1604087185525103E-4 0.0 0.0 0.0013405761426446977 3.2358734477630637E-4 26-27 4.1604087185525103E-4 0.0 0.0 0.002010864213967047 3.2358734477630637E-4 28-29 5.547211624736681E-4 0.0 0.0 0.0059863658783616686 3.2358734477630637E-4 30-31 6.009479260131404E-4 0.0 0.0 0.015601532694571915 3.4670072654604254E-4 32-33 6.240613077828766E-4 0.0 0.0 0.03372242400204507 4.1604087185525103E-4 34-35 7.396282166315574E-4 0.0 0.0 0.05933205100291275 4.622676353947234E-4 36-37 8.320817437105021E-4 0.0 0.0 0.10368663061903646 4.622676353947234E-4 38-39 9.707620343289192E-4 0.0 0.0 0.1656998339072386 5.31607780703932E-4 40-41 9.707620343289192E-4 0.0 0.0 0.23945463513446674 5.547211624736681E-4 42-43 0.0010401021796381277 0.0 0.0 0.3129783025439975 6.009479260131404E-4 44-45 0.0012018958520262808 0.0 0.0 0.4070728797285934 6.702880713223489E-4 46-47 0.0013868029061841701 0.0 0.0 0.5203746771638401 6.934014530920851E-4 48-49 0.0014792564332631148 0.0 0.0 0.6392467996055933 7.396282166315574E-4 50-51 0.0014792564332631148 0.0 0.0 0.7689590980973526 7.396282166315574E-4 52-53 0.0014792564332631148 0.0 0.0 0.8986945099708817 7.858549801710297E-4 54-55 0.0015254831968025873 0.0 0.0 1.0457418447899434 8.551951254802383E-4 56-57 0.0015717099603420595 0.0 0.0 1.2274361388818393 8.783085072499745E-4 58-59 0.0015717099603420595 0.0 0.0 1.4204328766591363 8.783085072499745E-4 60-61 0.0015948233421117957 0.0 0.0 1.638160932930051 8.783085072499745E-4 62-63 0.001617936723881532 0.0 0.0 1.8549182271666367 8.783085072499745E-4 64-65 0.0017103902509604766 0.0 0.0 2.0930091727766893 8.783085072499745E-4 66-67 0.0018490705415788937 0.0 0.0 2.3641291409356944 8.783085072499745E-4 68-69 0.0018490705415788937 0.0 0.0 2.67900274078481 8.783085072499745E-4 70-71 0.0019184106868881021 0.0 0.0 3.017868030910912 8.783085072499745E-4 72-73 0.0019415240686578383 0.0 0.0 3.358767298632751 0.0010632155614078637 74-75 0.0019415240686578383 0.0 0.0 3.7253686468825364 0.0010632155614078637 76-77 0.0019415240686578383 0.0 0.0 4.117071127734255 0.0010632155614078637 78-79 0.0019415240686578383 0.0 0.0 4.57656515731661 0.0010632155614078637 80-81 0.0022188846498946723 0.0 0.0 5.0769236458678595 0.0010863289431776 82-83 0.0022651114134341448 0.0 0.0 5.574647208897358 0.0011094423249473362 84-85 0.0023113381769736168 0.0 0.0 6.088503912402132 0.0011325557067170724 86-87 0.0024037917040525617 0.0 0.0 6.624641915932932 0.0011556690884868086 88-89 0.0024037917040525617 0.0 0.0 7.218424693597455 0.0011556690884868086 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8155 0.0 23.04615 12-13 CCTATAC 510 0.0 22.333057 3 GTACTTT 8625 0.0 21.350098 14-15 GTACATG 8795 0.0 20.36424 1 AGTACTT 8615 0.0 19.804815 12-13 TACATGG 8715 0.0 19.60389 2 AGAGTAC 13775 0.0 19.156246 10-11 CGTACAC 125 0.006063912 18.983099 3 ACTTTTT 9970 0.0 18.564644 16-17 CTATGCG 155 9.6859783E-4 18.370739 9 ACATGGG 8825 0.0 17.90757 3 CCCTATA 640 0.0 17.796656 2 TAACACG 160 0.0011986081 17.796656 5 TTTGCGT 480 0.0 17.796656 3 GGTATCA 19550 0.0 17.542921 1 TACTTTT 9690 0.0 17.362797 14-15 CTATACT 745 0.0 17.199451 4 GTAAGCG 290 0.0 17.183573 94-95 GTATCAA 23300 0.0 17.050076 1 GTGTACG 225 3.4554698E-5 16.9365 1 >>END_MODULE