FastQCFastQC Report
Fri 27 May 2016
SRR938946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173274
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98130.4515307319739711No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.3962684870844634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69950.32186461532232014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59490.2737344669839146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.21221438253989142No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44270.20370188020470498No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36420.16758126218783273No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29160.13417544221299293No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25620.11788665396079831No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25420.11696638343807546No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24710.1136994230824093No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22050.10145982513019527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG93550.023.8828562
GTACATG95100.023.8323171
GAGTACT74750.023.11105312-13
ACATGGG96150.021.760113
GTACTTT81550.021.03934114-15
AGTACTT80900.020.18406512-13
AGAGTAC132800.020.06542610-11
CATGGGG47950.019.7437424
GTATCAA230500.018.5752941
ACTTTTT98650.017.7530416-17
TACTTTT91050.017.6225114-15
CATGGGA49350.016.6897644
GTATATG8000.016.595371
TATCAAC257850.016.5587482
TCAACGC259200.016.3811934
CAACGCA258050.016.3804455
ATCAACG260250.016.2787253
AACGCAG260350.016.234246
GGTATCA189000.016.0559881
TATAGGG6550.015.8989882
Produced by FastQC (version 0.11.5)