FastQCFastQC Report
Fri 27 May 2016
SRR938946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173274
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT109360.5032039218248596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88210.4058853140469172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.34671191943583735No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54070.24879513581812512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.22813506258299693No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40390.1858486320638815No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34290.15778038112083426No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32000.14724328363565756No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28820.13261098232436408No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27410.1261230751391679No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25450.1171044240164839No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24310.11185888203696359No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24010.11047847625287929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT80500.022.37226512-13
TACATGG89700.021.0030382
GTACATG91600.020.8552271
GTACTTT84950.020.80927714-15
AGTACTT85450.020.63197312-13
AGAGTAC135650.020.60580310-11
GTATATG7250.020.3699361
ACATGGG91350.019.6366463
TAAGGTG7550.019.4849265
TATTCCG2854.3237378E-818.3160535
TACTTTT95000.018.28317314-15
CATGGGG45000.018.0329884
CCGCCTA1851.5334248E-417.955629
ACTTTTT99900.017.86102716-17
GTATCAA239500.017.1660841
GTATTAG5650.016.8634931
GTAAGGT5250.016.2703634
GTAAGTA5550.016.2459342
CCGTTGC3803.965397E-916.234319
CTAATAG3553.0171577E-816.0412043
Produced by FastQC (version 0.11.5)