##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938946_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173274 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13001673972081 33.0 31.0 34.0 30.0 34.0 2 32.355570443487565 34.0 31.0 34.0 30.0 34.0 3 32.44658749886116 34.0 31.0 34.0 30.0 34.0 4 36.037044569621685 37.0 35.0 37.0 35.0 37.0 5 35.947779709323356 37.0 35.0 37.0 35.0 37.0 6 35.993778971266394 37.0 35.0 37.0 35.0 37.0 7 35.932283273991224 37.0 35.0 37.0 35.0 37.0 8 35.896135968129194 37.0 35.0 37.0 35.0 37.0 9 37.561882210894716 39.0 37.0 39.0 35.0 39.0 10-11 37.4813003330459 39.0 37.0 39.0 34.5 39.0 12-13 37.411475727404834 39.0 37.0 39.0 34.0 39.0 14-15 38.64689105009309 40.0 38.0 41.0 34.0 41.0 16-17 38.6384040852649 40.0 38.0 41.0 34.0 41.0 18-19 38.745432927463355 40.0 38.0 41.0 34.0 41.0 20-21 38.74039352608093 40.0 38.0 41.0 34.0 41.0 22-23 38.67930504851206 40.0 38.0 41.0 34.0 41.0 24-25 38.60823784759768 40.0 38.0 41.0 34.0 41.0 26-27 38.44540311069842 40.0 38.0 41.0 34.0 41.0 28-29 38.26745454093685 40.0 38.0 41.0 33.5 41.0 30-31 38.08747723480795 40.0 37.5 41.0 33.0 41.0 32-33 37.914636856650375 40.0 37.0 41.0 33.0 41.0 34-35 37.87629539579455 40.0 37.0 41.0 33.0 41.0 36-37 37.769685966886826 40.0 37.0 41.0 33.0 41.0 38-39 37.638518889012616 40.0 37.0 41.0 33.0 41.0 40-41 37.47659061857824 40.0 37.0 41.0 32.0 41.0 42-43 37.29258643870952 40.0 36.5 41.0 31.5 41.0 44-45 37.018812860228394 40.0 36.0 41.0 31.0 41.0 46-47 36.68192919990761 39.5 36.0 41.0 30.5 41.0 48-49 36.358059545183906 39.0 35.5 41.0 30.0 41.0 50-51 36.070666883236996 39.0 35.0 41.0 29.0 41.0 52-53 35.84155955484674 39.0 35.0 40.0 28.5 41.0 54-55 35.58965942628495 38.5 35.0 40.0 28.0 41.0 56-57 35.27566542460822 38.0 34.5 40.0 27.5 41.0 58-59 34.95067626079363 38.0 34.0 40.0 27.0 41.0 60-61 34.51496612944341 37.5 34.0 40.0 26.0 41.0 62-63 34.19065037358382 37.0 34.0 40.0 26.0 41.0 64-65 33.921374157147234 36.5 33.0 39.0 26.0 41.0 66-67 33.44583678818225 36.0 33.0 39.0 24.5 40.5 68-69 33.01432677149775 35.5 32.5 39.0 23.5 40.0 70-71 32.53584752773925 35.0 32.0 38.0 22.5 40.0 72-73 32.02069458337973 35.0 31.0 37.0 21.5 39.0 74-75 31.565145950303553 35.0 31.0 36.5 20.0 39.0 76-77 29.619408781405383 32.5 28.5 35.0 18.0 37.0 78-79 30.603404356744708 34.0 30.0 35.0 18.0 37.0 80-81 30.612294860197103 34.0 31.0 35.0 18.0 37.0 82-83 30.29423119220126 34.0 30.0 35.0 16.0 37.0 84-85 29.90321998054548 34.0 30.0 35.0 9.5 36.0 86-87 29.347756656546757 34.0 29.0 35.0 4.5 36.0 88-89 28.78446965269911 34.0 29.0 35.0 2.0 35.0 90-91 28.322925917302648 33.5 28.5 35.0 2.0 35.0 92-93 27.97307771592537 33.0 27.0 35.0 2.0 35.0 94-95 27.563572747844958 33.0 27.0 35.0 2.0 35.0 96-97 26.928332782704803 33.0 25.5 35.0 2.0 35.0 98-99 26.019531361439007 32.5 24.5 34.0 2.0 35.0 100-101 21.707815259373646 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07585060626917084 1101 2 0.12398722167598208 1101 3 0.1800972170368027 1101 4 0.06877344381373973 1101 5 0.06339179846633414 1101 6 0.0772805546440054 1101 7 0.12005095818403078 1101 8 0.1913796001580934 1101 9 0.18070409173270008 1101 10-11 0.24350857932169845 1101 12-13 0.22047635522560682 1101 14-15 0.2657608302461085 1101 16-17 0.2510353641186427 1101 18-19 0.35401834560309453 1101 20-21 0.30482950918338503 1101 22-23 0.21041292758732055 1101 24-25 0.26207863888664207 1101 26-27 0.18962746581559742 1101 28-29 0.37235430625991484 1101 30-31 0.3622701727204145 1101 32-33 0.41687546515463936 1101 34-35 0.4415745318084845 1101 36-37 0.5114536566344583 1101 38-39 0.41746285904510216 1101 40-41 0.4221221086061746 1101 42-43 0.4009438003760195 1101 44-45 0.4384313387417791 1101 46-47 0.40961901405380274 1101 48-49 0.26726404385753 1101 50-51 0.37257346094514787 1101 52-53 0.45864992814470185 1101 54-55 0.4681689505758797 1101 56-57 0.2341979603209694 1101 58-59 0.2843542331889779 1101 60-61 0.49739819543424346 1101 62-63 0.44189896888057234 1101 64-65 0.5920654212821574 1101 66-67 0.630362436828392 1101 68-69 0.44686774279393404 1101 70-71 0.6126110082414442 1101 72-73 0.4685944490205003 1101 74-75 0.4857046615107272 1101 76-77 0.4745175609646921 1101 78-79 0.6524483378462271 1101 80-81 0.6555572459933465 1101 82-83 0.45537016858149926 1101 84-85 0.5311399022800138 1101 86-87 0.8413244604430332 1101 88-89 0.9864407676183156 1101 90-91 0.9486800196863605 1101 92-93 0.991760891058501 1101 94-95 0.7371592691056215 1101 96-97 0.5582625572830047 1101 98-99 0.517734406661571 1101 100-101 1.4270292515115273 1102 1 0.16151943215803755 1102 2 0.1752296707320582 1102 3 0.1824557741199513 1102 4 0.10334442208910843 1102 5 0.15689730551069658 1102 6 0.22405269192980626 1102 7 0.19363014743122875 1102 8 0.2182537185075617 1102 9 0.2891279264482307 1102 10-11 0.3114725718894249 1102 12-13 0.2569988554490905 1102 14-15 0.32436671937289674 1102 16-17 0.37674648055172355 1102 18-19 0.28650070542214934 1102 20-21 0.3661952542728031 1102 22-23 0.4094544231821189 1102 24-25 0.32572191644923265 1102 26-27 0.30853239550582856 1102 28-29 0.40342126891736285 1102 30-31 0.35797072966302323 1102 32-33 0.5267498554982311 1102 34-35 0.594493664467528 1102 36-37 0.4466360002485956 1102 38-39 0.4706089084141851 1102 40-41 0.5306182440429978 1102 42-43 0.38854368890144997 1102 44-45 0.5458111749430046 1102 46-47 0.5780318925211887 1102 48-49 0.47785632658609245 1102 50-51 0.3928452686352699 1102 52-53 0.4818394583310308 1102 54-55 0.6158501648646961 1102 56-57 0.49309357815631927 1102 58-59 0.4134152033714429 1102 60-61 0.4365330581489104 1102 62-63 0.5301089559883465 1102 64-65 0.45947498084767346 1102 66-67 0.5775445490802227 1102 68-69 0.5277616139394752 1102 70-71 0.4702692918486093 1102 72-73 0.5286499377327445 1102 74-75 0.4941332525381732 1102 76-77 0.3171247464852378 1102 78-79 0.3065465100361884 1102 80-81 0.5656097709735413 1102 82-83 0.49040781514880294 1102 84-85 0.5757489692923805 1102 86-87 0.5747732120187123 1102 88-89 0.7171640807648103 1102 90-91 0.8175358326882538 1102 92-93 0.9292588893443146 1102 94-95 0.9635491996825927 1102 96-97 0.8279885841564081 1102 98-99 0.8710515398531236 1102 100-101 1.2843579394338924 1103 1 0.009445562743159996 1103 2 -0.04440103869456635 1103 3 0.055571465579262735 1103 4 0.02975327466668176 1103 5 -0.021734861641881764 1103 6 -0.06584408073274517 1103 7 -0.010739640350990953 1103 8 0.06524616997319299 1103 9 -0.03605513681029748 1103 10-11 0.034883904861146675 1103 12-13 0.03285159687788308 1103 14-15 0.08770731308546686 1103 16-17 -0.06764452335814752 1103 18-19 -0.08016751502216835 1103 20-21 -0.0014299519558775842 1103 22-23 -0.02135162016545422 1103 24-25 1.387373821160054E-6 1103 26-27 -0.12951213293677455 1103 28-29 0.09932155594247405 1103 30-31 0.08698343108910933 1103 32-33 0.042113185215491455 1103 34-35 0.06686396640889569 1103 36-37 0.14866805891923462 1103 38-39 0.09345339504604766 1103 40-41 0.07696390024567279 1103 42-43 0.18560267030132138 1103 44-45 0.3618735633535195 1103 46-47 0.38625476638834755 1103 48-49 0.2236180448946783 1103 50-51 0.17944944518960426 1103 52-53 0.29524771199795197 1103 54-55 0.4136295340874696 1103 56-57 0.18842320502368182 1103 58-59 0.18404089550876535 1103 60-61 0.04387931891235297 1103 62-63 0.18921720061509717 1103 64-65 0.2969102871961269 1103 66-67 0.2639152352284384 1103 68-69 0.3110124558044589 1103 70-71 0.08814168997729155 1103 72-73 0.1527014270992595 1103 74-75 0.1295753446574146 1103 76-77 0.15203022030266666 1103 78-79 0.35926336089613287 1103 80-81 0.0976896294950258 1103 82-83 0.21261497043616728 1103 84-85 0.3051218646299212 1103 86-87 0.08719778317607663 1103 88-89 0.43671568878343336 1103 90-91 0.46695062352153016 1103 92-93 0.31774921072203455 1103 94-95 0.13655316040309273 1103 96-97 0.12554901198202373 1103 98-99 0.2982853071141811 1103 100-101 0.663596221125303 1104 1 0.008924292719775906 1104 2 0.0065161023553983455 1104 3 -0.009389627635222553 1104 4 0.07135466766199272 1104 5 0.10907633380404036 1104 6 0.05791204212149381 1104 7 -0.002208095700126478 1104 8 0.05139895817024609 1104 9 0.05139135716613907 1104 10-11 0.011587179944626769 1104 12-13 0.010319769067983486 1104 14-15 0.07260749033565617 1104 16-17 0.10404315959200261 1104 18-19 0.09009334063292584 1104 20-21 0.10125840395663488 1104 22-23 0.0035259227496808876 1104 24-25 0.09743613208940616 1104 26-27 -0.03873162530987173 1104 28-29 -0.087177274647523 1104 30-31 -0.014411402913509619 1104 32-33 -0.02507686597377301 1104 34-35 0.02797051142750462 1104 36-37 -0.08872860642356528 1104 38-39 0.06600351787086822 1104 40-41 0.13362834209142704 1104 42-43 0.12862013422612506 1104 44-45 0.16635501459868607 1104 46-47 0.00889847878703165 1104 48-49 -0.05996709771450526 1104 50-51 -0.050264233229995625 1104 52-53 0.0016632864798040714 1104 54-55 -0.06561481744159181 1104 56-57 -0.20013717036428602 1104 58-59 -0.14006586647585095 1104 60-61 -0.10401611082743045 1104 62-63 0.01666579070379015 1104 64-65 -0.07473409448752477 1104 66-67 0.01219467641225691 1104 68-69 0.013252354014305467 1104 70-71 -0.31258716870796377 1104 72-73 -0.25494322303386596 1104 74-75 -0.2346282981650596 1104 76-77 -0.21250484728775731 1104 78-79 -0.10329069673592173 1104 80-81 -0.11998151623258124 1104 82-83 0.0824206571519639 1104 84-85 0.08826342207810356 1104 86-87 -0.03329005937279561 1104 88-89 -0.06527377917179322 1104 90-91 0.003858287937632099 1104 92-93 0.058465180155568675 1104 94-95 0.13907171283531738 1104 96-97 0.2441204747006438 1104 98-99 0.28262054533550085 1104 100-101 0.8564160253410655 1105 1 -0.015014882683338726 1105 2 -0.023077739854805657 1105 3 0.026727765943157067 1105 4 0.015333703757967498 1105 5 -0.012856686665401185 1105 6 0.055066188579644404 1105 7 -0.005939345733594337 1105 8 0.045557304906509444 1105 9 -0.04985712328998204 1105 10-11 -0.05414146031128553 1105 12-13 0.003273860545867535 1105 14-15 0.08889941055715411 1105 16-17 0.07828566362180567 1105 18-19 0.02218445614324338 1105 20-21 0.06312744738925602 1105 22-23 -0.19390790011032522 1105 24-25 -0.058844381511960364 1105 26-27 -0.05783838341990588 1105 28-29 -0.03127635694119846 1105 30-31 0.10611988853366938 1105 32-33 -0.004351269268305202 1105 34-35 -0.05687693618504852 1105 36-37 -0.03128783962736037 1105 38-39 0.07820293379479892 1105 40-41 0.011388512101838444 1105 42-43 0.04186935191763297 1105 44-45 -0.015729406885746755 1105 46-47 0.02477827014763534 1105 48-49 0.07147099325641904 1105 50-51 0.0913768920089808 1105 52-53 0.1421934265545275 1105 54-55 0.21195631327756104 1105 56-57 0.15755765702802904 1105 58-59 0.10013965796984081 1105 60-61 0.10596410163202563 1105 62-63 0.017108042681208246 1105 64-65 -0.009110773572359676 1105 66-67 0.18208347525411028 1105 68-69 -0.017956310375801365 1105 70-71 0.09402492230937298 1105 72-73 0.1858994387964401 1105 74-75 0.2647955693396433 1105 76-77 0.1587406967223366 1105 78-79 0.15602247835659 1105 80-81 0.02699831779127848 1105 82-83 0.1577971737950996 1105 84-85 -0.003668982540951049 1105 86-87 0.023802171808512185 1105 88-89 0.17040234421963518 1105 90-91 0.19686044252143375 1105 92-93 0.22930542321119063 1105 94-95 0.23247769568790133 1105 96-97 0.2083743764252013 1105 98-99 0.22806892011526614 1105 100-101 0.9149514885051815 1106 1 0.024051749667250988 1106 2 -0.061224938065635115 1106 3 -0.05489730727433084 1106 4 -0.034022869841138004 1106 5 0.04029344024490911 1106 6 0.01229874506804407 1106 7 0.05048604342844243 1106 8 0.03601551325240848 1106 9 -0.03469489108848478 1106 10-11 0.017644070138317147 1106 12-13 0.15403687550885792 1106 14-15 0.10897120806864535 1106 16-17 0.07518767643708912 1106 18-19 0.005541677606174744 1106 20-21 0.051316135509388516 1106 22-23 0.1780560147621415 1106 24-25 0.0998724931219499 1106 26-27 0.11429637225555922 1106 28-29 0.12016403071211101 1106 30-31 0.24305700136847008 1106 32-33 0.35088622163497263 1106 34-35 0.1891699851305617 1106 36-37 0.029154748987984647 1106 38-39 0.04499281291938928 1106 40-41 0.18107114757637532 1106 42-43 0.2938930388840362 1106 44-45 0.3025620963358193 1106 46-47 0.2771407543947788 1106 48-49 0.29496604109183977 1106 50-51 0.15347131150524262 1106 52-53 0.432329034215293 1106 54-55 0.3501450013976921 1106 56-57 0.44521163562190225 1106 58-59 0.5468608457793493 1106 60-61 0.5328291507099294 1106 62-63 0.5126375604220286 1106 64-65 0.32495621377211137 1106 66-67 0.5830071249461426 1106 68-69 0.4146361211547571 1106 70-71 0.365937141198593 1106 72-73 0.5054283175482794 1106 74-75 0.409640614512611 1106 76-77 0.2902523937705652 1106 78-79 0.39766836309008013 1106 80-81 0.4947131778349423 1106 82-83 0.705848479285077 1106 84-85 0.6173677327007105 1106 86-87 0.575476633549048 1106 88-89 0.7909518303659979 1106 90-91 1.0265226214738483 1106 92-93 0.9585300722109942 1106 94-95 0.9228236372204073 1106 96-97 0.7313072027089511 1106 98-99 0.9548762826882644 1106 100-101 1.4703928790161953 1107 1 0.0048979008043446015 1107 2 -0.044089948322813655 1107 3 -0.15916680251552862 1107 4 -0.07660820691929615 1107 5 -0.1584882447496767 1107 6 -0.06576608205669032 1107 7 -0.07030169021950172 1107 8 -0.01433644441581805 1107 9 -0.00968344531065668 1107 10-11 -0.004028730291338434 1107 12-13 -0.1413442028594929 1107 14-15 -0.08650792980015609 1107 16-17 -0.10442926419454324 1107 18-19 -0.1301093890522722 1107 20-21 -0.11935196774913948 1107 22-23 -0.07359988113410054 1107 24-25 -0.1838370834723122 1107 26-27 -0.11634177388061318 1107 28-29 -0.009998675421947212 1107 30-31 -0.11072670864834322 1107 32-33 -0.1687826872302196 1107 34-35 -0.13924052649571195 1107 36-37 -0.19467646100682856 1107 38-39 -0.06296266300486053 1107 40-41 -0.17719930734926947 1107 42-43 -0.25420575138529955 1107 44-45 -0.26481369496362817 1107 46-47 -0.3463248510428443 1107 48-49 -0.2866532503264736 1107 50-51 -0.147881381334102 1107 52-53 0.05971657944812847 1107 54-55 -0.17980914945766102 1107 56-57 -0.10796181633664759 1107 58-59 -0.21346522682210178 1107 60-61 -0.13958080183411425 1107 62-63 -0.3796786908215992 1107 64-65 -0.3192892013134241 1107 66-67 -0.24428830378627708 1107 68-69 -0.27239955445428876 1107 70-71 -0.20308321027482634 1107 72-73 -0.13839833614650132 1107 74-75 -0.20411214788184395 1107 76-77 -0.5163006442030031 1107 78-79 -0.0567958204297625 1107 80-81 0.0190699031098589 1107 82-83 -0.07138069600260977 1107 84-85 -0.018302923846714947 1107 86-87 -0.006867263650256916 1107 88-89 0.09775469445179041 1107 90-91 0.26004873545618423 1107 92-93 0.4476320408847805 1107 94-95 0.3716464829929542 1107 96-97 0.15510022342580854 1107 98-99 0.21724784785435958 1107 100-101 0.8974831641921526 1108 1 0.027318137072064985 1108 2 -0.0884587370326031 1108 3 -0.06640487751874247 1108 4 -0.051928760964521814 1108 5 -0.0439009011527034 1108 6 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-0.9574566054114797 2316 52-53 -1.063233563673954 2316 54-55 -1.2178942494476317 2316 56-57 -1.119759930752501 2316 58-59 -1.1951868875813076 2316 60-61 -1.0243154838612583 2316 62-63 -1.131534644153703 2316 64-65 -1.1359759114675825 2316 66-67 -1.2376914200719327 2316 68-69 -1.2042358287259418 2316 70-71 -1.208869092974453 2316 72-73 -1.096318868364449 2316 74-75 -1.1999261843807822 2316 76-77 -1.2673498501804268 2316 78-79 -1.3080163493533838 2316 80-81 -1.3207575322372236 2316 82-83 -1.5016728916767619 2316 84-85 -1.861971414217333 2316 86-87 -1.9035016954173898 2316 88-89 -2.225404457234454 2316 90-91 -2.3256044113291168 2316 92-93 -2.5826436107668904 2316 94-95 -2.7604169910273164 2316 96-97 -3.0261841610804474 2316 98-99 -3.4053605544093877 2316 100-101 -3.920590148634318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 14.0 5 41.0 6 124.0 7 278.0 8 674.0 9 1242.0 10 2249.0 11 3323.0 12 4856.0 13 6440.0 14 8112.0 15 10670.0 16 14086.0 17 19667.0 18 18867.0 19 13345.0 20 12374.0 21 12894.0 22 14104.0 23 15390.0 24 17633.0 25 21001.0 26 25489.0 27 30922.0 28 37452.0 29 45753.0 30 55801.0 31 67458.0 32 83660.0 33 106540.0 34 142895.0 35 205601.0 36 321841.0 37 484555.0 38 343861.0 39 24051.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.571095033753366 19.704361813539503 15.296860080021323 22.427683072685806 2 15.935910520256535 21.35308295226465 38.71444649869276 23.996560028786064 3 19.487050413339503 24.906063386393065 30.499007488241244 25.10787871202619 4 13.394123336495998 17.36002915417016 36.5725168570553 32.673330652278544 5 13.490267859895466 37.74361409548683 34.14133494197435 14.624783102643352 6 31.48245458234903 36.326114424596256 17.002181961409377 15.189249031645344 7 27.7857278925713 33.28434426584039 20.96974426602444 17.960183575563875 8 26.183536912510803 34.303543869755956 19.639861333637636 19.87305788409561 9 26.17769319469151 17.03807251179557 20.633753498178326 36.150480795334595 10-11 25.09651337107056 26.438428840541967 26.922445121968053 21.54261266641942 12-13 26.38157912900076 24.006452936905333 27.383109538880053 22.22885839521386 14-15 23.078880442249883 25.33142743291042 26.289071073704783 25.300621051134915 16-17 21.91048461425548 28.408467094573798 27.074014374108753 22.607033917061973 18-19 21.902323406988717 27.3119496207105 29.08418358660712 21.701543385693657 20-21 23.060621900413846 25.87145017149241 28.98523149865134 22.0826964294424 22-23 22.614819852443823 25.868413278767427 29.170159860192502 22.346607008596248 24-25 22.882871648949926 26.089830366534546 28.959647978119644 22.06765000639588 26-27 22.336783120766178 25.889188385817896 29.520644888771503 22.253383604644423 28-29 22.2077151799543 26.831499387559965 29.047717867144225 21.913067565341507 30-31 22.43543612080207 26.129931154562197 28.802097664629496 22.63253506000624 32-33 22.388939452641498 26.165683664369976 28.983528998184305 22.461847884804218 34-35 22.93860127432967 26.177296280497934 28.78149530121419 22.10260714395821 36-37 22.82661190031524 25.767465575266417 29.18565346769067 22.220269056727673 38-39 22.455985338225194 26.357209338713 28.99653589109053 22.190269431971274 40-41 22.556096380308702 26.383152403985967 28.880133847224215 22.180617368481116 42-43 22.21332622880894 26.363974355611813 28.381332586069885 23.04136682950936 44-45 22.65342914607363 26.167424435193652 28.416250741395327 22.76289567733739 46-47 23.208768181728246 26.55748235260555 27.996537010868888 22.237212454797316 48-49 22.861593040885488 26.59707858912742 27.97799624067935 22.56333212930775 50-51 22.367694821815046 26.857973603268636 28.315253364585903 22.459078210330418 52-53 22.818406698833186 27.115931999370535 27.3517283140552 22.713932987741074 54-55 22.557210917721374 26.803569177195328 27.458525708217184 23.18069419686611 56-57 22.811182575229815 26.86511226840242 27.333253883311535 22.990451273056227 58-59 23.10153680788084 26.56415145649116 27.402035917287808 22.93227581834019 60-61 22.63475395956231 26.811758588638057 28.14282493258851 22.410662519211126 62-63 22.28775570866812 26.858463313875745 28.186390671401767 22.66739030605437 64-65 22.20450441351356 27.221762980506092 27.645847717389728 22.92788488859062 66-67 22.52271093242225 26.900005176608634 27.273618276879542 23.303665614089578 68-69 22.302386855362233 26.84471451788945 27.694806270464873 23.15809235628344 70-71 22.795745037211137 26.6464329854404 27.35971166083982 23.19811031650864 72-73 22.545155373873705 26.465369760094674 27.705710370620544 23.28376449541107 74-75 22.170927365808453 27.43929205429228 27.03556937597376 23.354211203925505 76-77 22.369807395244933 26.693267471693456 27.241776624185544 23.69514850887607 78-79 22.40514413526891 26.72754571335098 27.476347864578326 23.390962286801784 80-81 22.694745347342305 26.62694625712174 27.24115781075005 23.437150584785904 82-83 22.425704260024276 26.46164266447765 27.673362861746835 23.439290213751235 84-85 22.185260579199863 26.72992452861443 27.42233606991111 23.662478822274597 86-87 22.150403754241676 26.71284511725554 27.329604835097587 23.807146293405193 88-89 22.086607895922636 26.60782659934605 27.061801712285245 24.24376379244607 90-91 22.15025784137175 26.218724369085628 27.28109222523833 24.349925564304293 92-93 22.30277912495157 26.441534753556155 27.189806715582108 24.065879405910163 94-95 22.13848782988247 26.20500222245331 27.356076592275063 24.30043335538915 96-97 21.657386085593316 26.20163067973057 27.70305790985961 24.437925324816504 98-99 21.732418461731008 26.465852902119106 27.063246511944648 24.738482124205234 100-101 21.859591177894306 25.54282306646028 27.744415322914616 24.8531704327308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 994.0 1 689.5 2 458.5 3 536.0 4 480.5 5 499.0 6 791.5 7 1563.0 8 2612.5 9 3103.0 10 2794.5 11 2449.5 12 2498.0 13 2676.5 14 2878.0 15 3094.5 16 3220.0 17 3339.0 18 3519.0 19 3767.0 20 4252.0 21 4974.0 22 5903.5 23 7277.5 24 8723.5 25 10254.5 26 12384.5 27 14977.0 28 17467.0 29 20315.0 30 23392.0 31 27262.0 32 31449.5 33 34690.0 34 37274.5 35 40431.0 36 45809.5 37 49755.0 38 52250.5 39 54489.5 40 55938.5 41 58001.5 42 58636.0 43 59727.5 44 63555.0 45 67377.5 46 70201.5 47 80626.0 48 98759.5 49 98971.0 50 86262.5 51 81505.5 52 81439.0 53 82230.5 54 81061.0 55 76003.0 56 69432.5 57 63702.0 58 59543.5 59 53828.5 60 44251.0 61 35603.0 62 28365.5 63 21126.5 64 14722.0 65 10429.5 66 7631.0 67 5719.0 68 4272.5 69 3235.5 70 2459.5 71 1524.5 72 941.0 73 567.0 74 333.0 75 225.0 76 133.0 77 64.5 78 28.5 79 17.5 80 8.5 81 3.5 82 2.5 83 2.5 84 1.5 85 2.0 86 1.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39576233829696583 2 0.0 3 0.0 4 0.0 5 5.061487874975728E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010583111011312886 16-17 0.002806825094304722 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002438716885215578 36-37 0.007523211523259377 38-39 0.012722739976643534 40-41 0.0023006763068071493 42-43 5.061487874975728E-4 44-45 3.220946829530009E-4 46-47 3.220946829530009E-4 48-49 4.141217352252869E-4 50-51 5.751690767017873E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.3804057840842895E-4 60-61 0.002484730411351721 62-63 0.0 64-65 2.530743937487864E-4 66-67 0.0016794937039692189 68-69 0.0017485139931734334 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12253402010054876 78-79 1.3804057840842895E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14457449911976122 88-89 0.4370824847672222 90-91 0.33638188281827325 92-93 0.0 94-95 0.0 96-97 3.911149721572153E-4 98-99 0.0 100-101 2.760811568168579E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173274.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.078665788765726 #Duplication Level Percentage of deduplicated Percentage of total 1 68.27411316349419 28.72883589831043 2 15.016943318415358 12.637858781288767 3 6.04757087184185 7.634211406503234 4 3.0891223133919574 5.199445816233561 5 1.8340526872502747 3.8587245032896 6 1.192861541511562 3.0116413282522148 7 0.8288353731117026 2.4413400661351994 8 0.5783756419187542 1.9469820269329683 9 0.4533832819215852 1.716998723477292 >10 2.4427308210850334 18.37561248851804 >50 0.16532411800312913 4.7546241256092365 >100 0.07100024238818588 5.322344673591634 >500 0.003171387390922822 0.8758313889073113 >1k 0.002077805531983912 1.990856365769234 >5k 3.280745576816703E-4 1.001456339872315 >10k+ 1.0935818589389009E-4 0.5032360673089021 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10936 0.5032039218248596 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8821 0.4058853140469172 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7535 0.34671191943583735 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5407 0.24879513581812512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4958 0.22813506258299693 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4039 0.1858486320638815 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3429 0.15778038112083426 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3200 0.14724328363565756 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2882 0.13261098232436408 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2741 0.1261230751391679 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2545 0.1171044240164839 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2431 0.11185888203696359 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2401 0.11047847625287929 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.202705227228596E-5 4.601352613614298E-5 5 0.0 0.0 0.0 9.202705227228596E-5 4.601352613614298E-5 6 0.0 0.0 0.0 9.202705227228596E-5 9.202705227228596E-5 7 0.0 0.0 0.0 9.202705227228596E-5 9.202705227228596E-5 8 0.0 0.0 0.0 1.3804057840842895E-4 9.202705227228596E-5 9 0.0 0.0 0.0 8.282434704505737E-4 9.202705227228596E-5 10-11 2.300676306807149E-5 0.0 0.0 8.512502335186452E-4 9.202705227228596E-5 12-13 9.202705227228596E-5 0.0 0.0 9.662840488590025E-4 9.202705227228596E-5 14-15 1.3804057840842895E-4 0.0 0.0 0.0010583111011312886 1.1503381534035747E-4 16-17 1.3804057840842895E-4 0.0 0.0 0.0010813178641993601 1.3804057840842895E-4 18-19 1.6104734147650044E-4 0.0 0.0 0.001173344916471646 1.8405410454457193E-4 20-21 1.8405410454457193E-4 0.0 0.0 0.0012653719687439318 1.8405410454457193E-4 22-23 1.8405410454457193E-4 0.0 0.0 0.00154145312556079 2.0706086761264342E-4 24-25 2.760811568168579E-4 0.0 0.0 0.0019095613346499338 2.300676306807149E-4 26-27 2.9908791988492937E-4 0.0 0.0 0.0028758453835089366 2.300676306807149E-4 28-29 4.831420244295013E-4 0.0 0.0 0.00648790718519616 2.300676306807149E-4 30-31 5.521623136337158E-4 0.0 0.0 0.01665689646128376 2.300676306807149E-4 32-33 5.751690767017872E-4 0.0 0.0 0.03697186825039089 2.300676306807149E-4 34-35 6.902028920421448E-4 0.0 0.0 0.06653555879286274 2.760811568168579E-4 36-37 7.822299443144306E-4 0.0 0.0 0.11349236221479667 2.760811568168579E-4 38-39 8.052367073825022E-4 0.0 0.0 0.18170741471162863 2.760811568168579E-4 40-41 8.282434704505737E-4 0.0 0.0 0.2571695975749031 2.760811568168579E-4 42-43 9.662840488590027E-4 0.0 0.0 0.33585272726770765 2.760811568168579E-4 44-45 0.0012423652056758605 0.0 0.0 0.4327802200734928 2.760811568168579E-4 46-47 0.0015414531255607898 0.0 0.0 0.5524844083166688 2.9908791988492937E-4 48-49 0.0016104734147650043 0.0 0.0 0.6757316380723277 3.220946829530009E-4 50-51 0.0016104734147650043 0.0 0.0 0.8101601546790695 3.220946829530009E-4 52-53 0.0016104734147650043 0.0 0.0 0.9450257997841045 3.220946829530009E-4 54-55 0.0016104734147650043 0.0 0.0 1.0976986795038268 3.220946829530009E-4 56-57 0.0016564869409011473 0.0 0.0 1.2844675820904312 3.220946829530009E-4 58-59 0.0016564869409011473 0.0 0.0 1.4864209483019628 3.220946829530009E-4 60-61 0.0016564869409011473 0.0 0.0 1.7059054679713648 3.220946829530009E-4 62-63 0.0016564869409011473 0.0 0.0 1.9326141112441413 3.220946829530009E-4 64-65 0.0016564869409011473 0.0 0.0 2.1711252239708383 3.220946829530009E-4 66-67 0.0017485139931734332 0.0 0.0 2.4446066165610043 3.220946829530009E-4 68-69 0.0017945275193095762 0.0 0.0 2.766264171015712 3.220946829530009E-4 70-71 0.001955574860786077 0.0 0.0 3.113022103977685 3.6810820908914386E-4 72-73 0.002047601913058363 0.0 0.0 3.4593889219675016 4.601352613614298E-4 74-75 0.002070608676126434 0.0 0.0 3.8257946305896082 4.601352613614298E-4 76-77 0.002070608676126434 0.0 0.0 4.219233285816699 4.8314202442950127E-4 78-79 0.002070608676126434 0.0 0.0 4.681347128801983 5.061487874975728E-4 80-81 0.002392703359079435 0.0 0.0 5.189658552027954 5.521623136337157E-4 82-83 0.002530743937487864 0.0 0.0 5.691252000438048 5.981758397698587E-4 84-85 0.0025537507005559358 0.0 0.0 6.205154067089562 5.981758397698587E-4 86-87 0.002576757463624007 0.0 0.0 6.759916144949969 5.981758397698587E-4 88-89 0.002576757463624007 0.0 0.0 7.359656444608457 5.981758397698587E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8050 0.0 22.372265 12-13 TACATGG 8970 0.0 21.003038 2 GTACATG 9160 0.0 20.855227 1 GTACTTT 8495 0.0 20.809277 14-15 AGTACTT 8545 0.0 20.631973 12-13 AGAGTAC 13565 0.0 20.605803 10-11 GTATATG 725 0.0 20.369936 1 ACATGGG 9135 0.0 19.636646 3 TAAGGTG 755 0.0 19.484926 5 TATTCCG 285 4.3237378E-8 18.316053 5 TACTTTT 9500 0.0 18.283173 14-15 CATGGGG 4500 0.0 18.032988 4 CCGCCTA 185 1.5334248E-4 17.95562 9 ACTTTTT 9990 0.0 17.861027 16-17 GTATCAA 23950 0.0 17.166084 1 GTATTAG 565 0.0 16.863493 1 GTAAGGT 525 0.0 16.270363 4 GTAAGTA 555 0.0 16.245934 2 CCGTTGC 380 3.965397E-9 16.23431 9 CTAATAG 355 3.0171577E-8 16.041204 3 >>END_MODULE