FastQCFastQC Report
Fri 27 May 2016
SRR938945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039634
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121870.597509160957309No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA107120.5251922648867394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103320.5065614713227962No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA88570.4342445752522266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62970.3087318607161873No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT62260.30525084402397684No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45680.22396175000024515No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37420.18346428820072622No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG36090.17694351045334605No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT35290.1730212381240948No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG28330.13889746885960913No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC27230.1335043444068887No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26310.12899373122824978No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23290.11418715318532639No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT21330.10457758597866088No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG20950.10271450662226655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129600.023.6562791
GAGTACT98350.023.17733212-13
TACATGG132650.022.743372
ACATGGG133950.021.7447813
GTACTTT107550.021.21853614-15
AGTACTT100700.020.61491612-13
TACCTGG13550.019.923162
GTATGCG1200.004788573419.77461
TTTGCGT6000.019.7338313
CTTTGCG6250.019.7022572
CATGGGG40600.019.364434
CATGGGA92150.019.1192234
ACTTTTT127650.018.3249816-17
CTATACG1300.007693824418.2158453
ATGGGAG30200.018.1917175
CGCAGAA4450.018.0863829
TTGCGTT7200.017.7604484
GTATAGG6050.017.2578321
GTATAGA5000.017.0852531
TACTTTT121650.017.06617714-15
Produced by FastQC (version 0.11.5)