FastQCFastQC Report
Fri 27 May 2016
SRR938945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039634
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117660.5768682028246245No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA107860.5288203667912968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105870.5190637143722844No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA91320.44772738638402776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.31750794505288693No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT57630.2825506929184354No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49590.24313185600946052No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA37780.18522931074888926No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG37780.18522931074888926No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT35400.17356055056936687No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC29390.144094479695867No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG28720.14080957662011911No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.12624814059777392No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24770.12144335699444116No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG23790.1166385733911084No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT22840.11198087500012258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1658.497227E-825.883716
GAGTACT107450.022.59119212-13
GTACATG133050.022.4147681
TACATGG134900.021.9151332
AGTACTT109050.021.45463812-13
ACATGGG132350.021.3334453
GTACTTT114450.021.27166714-15
CTTTGCG5000.019.9304582
GTATACG1200.00468411419.8501381
CATGGGA90450.019.4640564
GTAAGCG1503.767127E-918.98231994-95
ACCGTCG1250.00606695318.9813888
TATAACG1507.7749335E-418.9813882
ACTTTTT132850.018.46770716-17
GCACCGT4151.8189894E-1218.2953156
TACTTTT128450.018.04805414-15
ATGGGAG30450.017.9216735
TAGGTCG1350.0094560317.5753615
ATCACGC1350.0094560317.5753613
CATGGGG41250.017.4858864
Produced by FastQC (version 0.11.5)