##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938945_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2039634 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.163253309172134 33.0 31.0 34.0 30.0 34.0 2 32.401029302316005 34.0 31.0 34.0 31.0 34.0 3 32.47511661405919 34.0 31.0 34.0 30.0 34.0 4 36.03052116213007 37.0 35.0 37.0 35.0 37.0 5 35.96767410231443 37.0 35.0 37.0 35.0 37.0 6 36.0207900044812 37.0 35.0 37.0 35.0 37.0 7 35.95744138409146 37.0 35.0 37.0 35.0 37.0 8 35.93235207885336 37.0 35.0 37.0 35.0 37.0 9 37.59264946554136 39.0 37.0 39.0 35.0 39.0 10-11 37.504607444276765 39.0 37.0 39.0 34.5 39.0 12-13 37.413295473599675 39.0 37.0 39.0 34.0 39.0 14-15 38.66925610183003 40.0 38.0 41.0 34.0 41.0 16-17 38.766274488462145 40.0 38.0 41.0 34.5 41.0 18-19 38.857095194530004 40.0 38.0 41.0 35.0 41.0 20-21 38.84601134321157 40.0 38.0 41.0 34.0 41.0 22-23 38.77364885072518 40.0 38.0 41.0 34.0 41.0 24-25 38.69576845649759 40.0 38.0 41.0 34.0 41.0 26-27 38.50934260754626 40.0 38.0 41.0 34.0 41.0 28-29 38.34284827572006 40.0 38.0 41.0 34.0 41.0 30-31 38.121264403319415 40.0 37.5 41.0 33.0 41.0 32-33 37.97655780399817 40.0 37.0 41.0 33.0 41.0 34-35 37.86112778076851 40.0 37.0 41.0 33.0 41.0 36-37 37.699443135386055 40.0 37.0 41.0 33.0 41.0 38-39 37.52630643537027 40.0 37.0 41.0 33.0 41.0 40-41 37.322293852720634 40.0 36.0 41.0 32.0 41.0 42-43 37.11557637301594 40.0 36.0 41.0 31.0 41.0 44-45 36.81155589679325 40.0 36.0 41.0 31.0 41.0 46-47 36.385567214510054 39.5 35.5 41.0 30.0 41.0 48-49 35.968875788499304 39.0 35.0 41.0 28.5 41.0 50-51 35.680457376176314 39.0 35.0 40.5 28.0 41.0 52-53 35.37660089996538 39.0 35.0 40.0 27.0 41.0 54-55 35.07784656462876 38.0 34.5 40.0 26.0 41.0 56-57 34.971213953091585 38.0 34.5 40.0 26.0 41.0 58-59 34.70939148886516 38.0 34.0 40.0 26.0 41.0 60-61 34.352696611254764 38.0 34.0 40.0 25.5 41.0 62-63 33.92793143279627 37.0 33.5 40.0 23.5 41.0 64-65 33.49929521669084 36.5 33.0 39.5 22.5 41.0 66-67 33.045756493566984 36.0 32.5 39.0 22.0 40.0 68-69 32.58011339289304 35.5 32.0 39.0 20.5 40.0 70-71 32.0496140484028 35.0 31.0 38.0 18.5 40.0 72-73 31.531812815436496 35.0 31.0 37.0 17.5 39.5 74-75 31.00463735160328 35.0 30.5 37.0 13.5 39.0 76-77 29.117443129502647 32.5 28.5 35.0 8.0 37.0 78-79 30.040154753254754 34.0 29.5 36.0 7.5 37.5 80-81 30.026090710392157 34.0 30.0 35.0 7.0 37.0 82-83 29.622922543946608 34.0 29.5 35.0 2.0 37.0 84-85 29.177814745194482 34.0 29.0 35.0 2.0 36.0 86-87 28.4807264440581 34.0 28.5 35.0 2.0 36.0 88-89 27.766405149159112 33.0 26.5 35.0 2.0 35.0 90-91 27.176302218927514 33.0 25.5 35.0 2.0 35.0 92-93 26.787052480984332 32.5 25.0 34.0 2.0 35.0 94-95 26.246440783003223 32.0 25.0 34.0 2.0 35.0 96-97 25.389788805246432 32.0 21.5 34.0 2.0 35.0 98-99 24.19658379885803 31.5 9.5 34.0 2.0 35.0 100-101 18.781156325105385 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14553643270391348 1101 2 0.1586263866262385 1101 3 0.1464819169362599 1101 4 0.13993935264581836 1101 5 0.16061915969491025 1101 6 0.13807087435203869 1101 7 0.2386937291980118 1101 8 0.16289554920034277 1101 9 0.20329268548162105 1101 10-11 0.27470132385659696 1101 12-13 0.2047220273026653 1101 14-15 0.32476737436794423 1101 16-17 0.2372772159736698 1101 18-19 0.27925558237351567 1101 20-21 0.3388842857506873 1101 22-23 0.3640437270491148 1101 24-25 0.33493687981144404 1101 26-27 0.20548954397555264 1101 28-29 0.26864203993812197 1101 30-31 0.27644868822094537 1101 32-33 0.3377827055666671 1101 34-35 0.32684642602910685 1101 36-37 0.4370856180784415 1101 38-39 0.33842195173605205 1101 40-41 0.37399364479913544 1101 42-43 0.4523618204174227 1101 44-45 0.4429627422349469 1101 46-47 0.45210871398077757 1101 48-49 0.4140353354835682 1101 50-51 0.3451823325889123 1101 52-53 0.36883337871010013 1101 54-55 0.34459012488289176 1101 56-57 0.427450962956641 1101 58-59 0.4263000449716827 1101 60-61 0.38845721869625294 1101 62-63 0.4715463404273095 1101 64-65 0.4123172463516198 1101 66-67 0.33661104477914705 1101 68-69 0.4339884530551714 1101 70-71 0.454188407575856 1101 72-73 0.3803445317634946 1101 74-75 0.2726151311454572 1101 76-77 0.051008041339429155 1101 78-79 0.40524606961849585 1101 80-81 0.5156637395122381 1101 82-83 0.42792504671272624 1101 84-85 0.6509874884994389 1101 86-87 0.7407172049722028 1101 88-89 0.6203159186504088 1101 90-91 0.7045128527875626 1101 92-93 0.6089658010240662 1101 94-95 0.5933284168365951 1101 96-97 0.8499807372266481 1101 98-99 1.1715850738509523 1101 100-101 1.304701122157205 1102 1 0.12421863731958638 1102 2 0.13975990462056131 1102 3 0.18124837086451606 1102 4 0.14913617323748696 1102 5 0.13062373626129187 1102 6 0.1121855698185783 1102 7 0.15912860423217268 1102 8 0.15304154646293 1102 9 0.10845493514903382 1102 10-11 0.17677572316227952 1102 12-13 0.15486209474175894 1102 14-15 0.3036107444470062 1102 16-17 0.2764855765453049 1102 18-19 0.2322207072327913 1102 20-21 0.239710385285413 1102 22-23 0.27934488269873015 1102 24-25 0.4066419251695308 1102 26-27 0.25697191993275936 1102 28-29 0.2894776708782629 1102 30-31 0.1333124455979302 1102 32-33 0.2408695170105375 1102 34-35 0.270753519067668 1102 36-37 0.2538303634183592 1102 38-39 0.21102571432360406 1102 40-41 0.33414048917936867 1102 42-43 0.38807145193189996 1102 44-45 0.3499433645520824 1102 46-47 0.3598156464399267 1102 48-49 0.42466346522363807 1102 50-51 0.285046170415427 1102 52-53 0.39991287297554123 1102 54-55 0.3606106379165155 1102 56-57 0.3735664224273876 1102 58-59 0.18653152119937744 1102 60-61 0.322269093580708 1102 62-63 0.42978677052865066 1102 64-65 0.2234920599990602 1102 66-67 0.22829466711812074 1102 68-69 0.14577439015642568 1102 70-71 0.21319409391962552 1102 72-73 0.17500428083552677 1102 74-75 0.31605721520403307 1102 76-77 0.15590877673344394 1102 78-79 0.425894961732471 1102 80-81 0.40313201921876285 1102 82-83 0.5045006189300416 1102 84-85 0.5612704044808225 1102 86-87 0.6518287245467427 1102 88-89 0.5535330796555087 1102 90-91 0.5107936973265588 1102 92-93 0.570811009721357 1102 94-95 0.608867564593627 1102 96-97 0.7249664188073801 1102 98-99 0.9260321750836411 1102 100-101 1.074490642725916 1103 1 0.1335937066383721 1103 2 0.10715498634015574 1103 3 0.109847111982468 1103 4 0.10155575551849694 1103 5 0.12826029559870733 1103 6 0.13304669668595892 1103 7 0.1668653734536818 1103 8 0.15232749690408554 1103 9 0.15133540684425384 1103 10-11 0.1793805252511902 1103 12-13 0.1933682206743299 1103 14-15 0.20909371552694012 1103 16-17 0.20465625762816586 1103 18-19 0.25053961697629745 1103 20-21 0.1976952761657529 1103 22-23 0.18518593484441936 1103 24-25 0.23943760876107234 1103 26-27 0.24087253183943602 1103 28-29 0.27194331780611236 1103 30-31 0.3343982023161729 1103 32-33 0.317362076001487 1103 34-35 0.2587620285103327 1103 36-37 0.32371426698786365 1103 38-39 0.3109416867460837 1103 40-41 0.4287520557259299 1103 42-43 0.400616436525965 1103 44-45 0.40298504666693447 1103 46-47 0.45917759668250113 1103 48-49 0.4036230337517921 1103 50-51 0.3404505009781218 1103 52-53 0.49393318324909075 1103 54-55 0.6134293379871778 1103 56-57 0.5675328092838043 1103 58-59 0.5962520489622776 1103 60-61 0.4480099464051648 1103 62-63 0.598562312852458 1103 64-65 0.63056975936518 1103 66-67 0.6101057334074795 1103 68-69 0.5209892123887201 1103 70-71 0.6073108959493823 1103 72-73 0.6369259508664662 1103 74-75 0.5519103723910597 1103 76-77 0.309226888980767 1103 78-79 0.5650712485268841 1103 80-81 0.7431611515957322 1103 82-83 0.670176224947916 1103 84-85 0.6677126419910309 1103 86-87 0.8602054976553823 1103 88-89 0.828234335474324 1103 90-91 0.6434732950504731 1103 92-93 0.6207120885065827 1103 94-95 0.7647474352619277 1103 96-97 0.6003790306548957 1103 98-99 1.0073139362932189 1103 100-101 0.9438953620019817 1104 1 0.031648977876862716 1104 2 0.10711895350327438 1104 3 -0.017456900717803592 1104 4 -0.021316218245424068 1104 5 0.03867221881220928 1104 6 0.10908538237517718 1104 7 0.0634203209188513 1104 8 0.0908382440893476 1104 9 0.12456927562206488 1104 10-11 0.19080554561757168 1104 12-13 0.16239721405264618 1104 14-15 0.18750430186997136 1104 16-17 0.17894735072865586 1104 18-19 0.16929000863756727 1104 20-21 0.22073253098579215 1104 22-23 0.25722419231731664 1104 24-25 0.2365596817458453 1104 26-27 0.16200761842268463 1104 28-29 0.13176827132897273 1104 30-31 0.11591048481361099 1104 32-33 0.14017634500488896 1104 34-35 0.1744049796518965 1104 36-37 0.2937592297017133 1104 38-39 0.28635058111242984 1104 40-41 0.1806698904135402 1104 42-43 0.033903205686030446 1104 44-45 0.17421491527763067 1104 46-47 0.09477647287576474 1104 48-49 0.14629975131702366 1104 50-51 0.037524530180384374 1104 52-53 0.07895448962219831 1104 54-55 0.2974464248628763 1104 56-57 0.28620121318579805 1104 58-59 0.4531152599580395 1104 60-61 0.27939115293076355 1104 62-63 0.37542844825237154 1104 64-65 0.36247344202555354 1104 66-67 0.2230425662777833 1104 68-69 0.32079782058122674 1104 70-71 0.5776167050693459 1104 72-73 0.2822137435679899 1104 74-75 0.351384240751095 1104 76-77 0.18689854106022707 1104 78-79 0.3786663334390461 1104 80-81 0.3031694025931664 1104 82-83 0.28699624876092145 1104 84-85 0.27825825225151135 1104 86-87 0.3426992832965574 1104 88-89 0.5056331302020425 1104 90-91 0.6836773244616232 1104 92-93 0.5557965828979938 1104 94-95 0.6551355454701877 1104 96-97 0.6232520488488724 1104 98-99 0.7921080791294699 1104 100-101 0.8381695353355951 1105 1 -0.06135102212314081 1105 2 -0.02061574037427505 1105 3 0.03236962989443981 1105 4 0.06437765930559891 1105 5 -0.024011454657177467 1105 6 -0.06014931150237146 1105 7 0.08048154540864516 1105 8 0.06933824408935152 1105 9 -0.05176745907181157 1105 10-11 0.09463717827063789 1105 12-13 0.13242272425672041 1105 14-15 0.08921858758425572 1105 16-17 0.021080003789883506 1105 18-19 0.08541755965797648 1105 20-21 0.11611518404701826 1105 22-23 0.016433375990779098 1105 24-25 -0.09543521621333895 1105 26-27 -0.025165850965073844 1105 28-29 0.01759480194122176 1105 30-31 0.034660484813613834 1105 32-33 0.0737732837804046 1105 34-35 0.1770427347539396 1105 36-37 0.16315208684456906 1105 38-39 0.10526384641855202 1105 40-41 0.11932805367884924 1105 42-43 0.33787769548195 1105 44-45 0.3651587928286446 1105 46-47 0.2003938198145434 1105 48-49 0.3423507717251866 1105 50-51 0.19155004038447032 1105 52-53 0.11139326513239922 1105 54-55 0.2103443840465431 1105 56-57 0.39296141726742917 1105 58-59 0.30740097424374824 1105 60-61 0.26212074476749336 1105 62-63 0.07749987682380066 1105 64-65 0.12885609508678186 1105 66-67 0.19472113770635247 1105 68-69 0.10563455527510257 1105 70-71 0.26095854180403677 1105 72-73 0.39418823336390574 1105 74-75 0.3156240366694618 1105 76-77 0.0911332349377787 1105 78-79 0.23079898650027175 1105 80-81 0.19043981075643046 1105 82-83 0.26463400386296243 1105 84-85 0.32302866041477785 1105 86-87 0.45884214043941896 1105 88-89 0.4386739465285707 1105 90-91 0.37678956935958396 1105 92-93 0.6224802563673819 1105 94-95 0.7651967699599851 1105 96-97 0.9234816406856048 1105 98-99 0.9733172628029401 1105 100-101 0.4772919843151868 1106 1 0.07957479534879752 1106 2 0.10880544068965747 1106 3 0.05730057181732917 1106 4 0.04439278863698348 1106 5 0.11147976056976461 1106 6 0.10217011260694875 1106 7 0.16007598171903936 1106 8 0.13270012793513075 1106 9 0.07732746528152745 1106 10-11 0.18253969169182227 1106 12-13 0.17085305612592805 1106 14-15 0.21975348265502959 1106 16-17 0.1701273439558193 1106 18-19 0.16666316727144448 1106 20-21 0.21648792684838725 1106 22-23 0.2201235907296777 1106 24-25 0.27906100145381885 1106 26-27 0.16013014708276074 1106 28-29 0.20788006304419326 1106 30-31 0.24078237353981535 1106 32-33 0.27551376694292173 1106 34-35 0.22103773231371804 1106 36-37 0.2976749028523784 1106 38-39 0.4144992051301202 1106 40-41 0.4489733684665467 1106 42-43 0.35128313716082005 1106 44-45 0.4344639416817486 1106 46-47 0.44246299990238924 1106 48-49 0.4635225628428046 1106 50-51 0.3701140960213607 1106 52-53 0.21924367996891192 1106 54-55 0.30474067492014 1106 56-57 0.2803241551883673 1106 58-59 0.2939444100661035 1106 60-61 0.45603667936974546 1106 62-63 0.48366678751194314 1106 64-65 0.538408193671458 1106 66-67 0.3982412694779498 1106 68-69 0.349512300516686 1106 70-71 0.25933152862687336 1106 72-73 0.45582842370065535 1106 74-75 0.4140816989437468 1106 76-77 0.2217420685151339 1106 78-79 0.5086945453094494 1106 80-81 0.6061053793264648 1106 82-83 0.6473918125501257 1106 84-85 0.7790346389408853 1106 86-87 0.8938270111080371 1106 88-89 0.8684291929902592 1106 90-91 1.0603425220194147 1106 92-93 0.9099778161526437 1106 94-95 1.0056589466315309 1106 96-97 1.0378246128671584 1106 98-99 1.0401892735398839 1106 100-101 1.4920774894396374 1107 1 0.01591095246141805 1107 2 0.02744682072054161 1107 3 0.034133559513207956 1107 4 0.10658391541507939 1107 5 0.0035843420192662734 1107 6 0.002164960247384329 1107 7 0.002380861146669133 1107 8 -0.08030056333978308 1107 9 -0.020419951740436204 1107 10-11 -0.01830930266778097 1107 12-13 0.0629762433183032 1107 14-15 0.09301330899187832 1107 16-17 0.05079261376055655 1107 18-19 0.06432836122200314 1107 20-21 0.015476132238610774 1107 22-23 -0.03989238158497699 1107 24-25 0.027809407442575207 1107 26-27 0.03551889976807132 1107 28-29 -0.010507348596412669 1107 30-31 0.10983765978917859 1107 32-33 0.11459806383905402 1107 34-35 0.19552782761806498 1107 36-37 0.19529358244574269 1107 38-39 0.18009546909694762 1107 40-41 0.10876378192909186 1107 42-43 0.14599524191400803 1107 44-45 -0.019207042948472974 1107 46-47 -0.16552186933501645 1107 48-49 -0.04021325564529832 1107 50-51 -0.04229722256763324 1107 52-53 -0.15155052763396526 1107 54-55 -0.003794423382579737 1107 56-57 0.1668556010406519 1107 58-59 -0.010494920184399348 1107 60-61 0.1046292980421768 1107 62-63 0.09721835189711925 1107 64-65 0.021403260287165438 1107 66-67 -0.06904303629168851 1107 68-69 -0.08943044961248603 1107 70-71 -0.07470739172480023 1107 72-73 -0.0025906033907361348 1107 74-75 -0.05128871992877748 1107 76-77 -0.20097575821960234 1107 78-79 0.0327696609870749 1107 80-81 -0.005316543104761706 1107 82-83 -0.1610402385612808 1107 84-85 0.10772440821536122 1107 86-87 0.002212619422799378 1107 88-89 0.37968030038976863 1107 90-91 0.42024020474569923 1107 92-93 0.5097994157026697 1107 94-95 0.6236415402434616 1107 96-97 0.5991133611157125 1107 98-99 0.7534103713073392 1107 100-101 0.9886590419886936 1108 1 5.780879824399676E-4 1108 2 0.06295590366795523 1108 3 0.08646389837106483 1108 4 0.02019012788277763 1108 5 -0.04290964470242642 1108 6 0.003019366225132103 1108 7 0.015337754558970573 1108 8 0.04371657490885639 1108 9 0.08244345093321215 1108 10-11 0.09770266343905831 1108 12-13 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-0.9452940987020142 2316 54-55 -0.940117489591799 2316 56-57 -0.963533680771782 2316 58-59 -1.0074083939479692 2316 60-61 -0.9388133510930672 2316 62-63 -1.0543606896249997 2316 64-65 -1.1457087197280345 2316 66-67 -1.1293306052130347 2316 68-69 -1.101336032960191 2316 70-71 -0.8607653144051142 2316 72-73 -0.9274495662003623 2316 74-75 -1.1104821724808644 2316 76-77 -1.0899288565654892 2316 78-79 -1.092015828314544 2316 80-81 -1.1189583373917174 2316 82-83 -1.1522761268559911 2316 84-85 -1.3224642589752023 2316 86-87 -1.3804955501923892 2316 88-89 -1.4460025240596615 2316 90-91 -1.6347572715990246 2316 92-93 -1.7289195257676937 2316 94-95 -1.5131539925672506 2316 96-97 -1.3850668342599306 2316 98-99 -1.5903248940598047 2316 100-101 -1.0185892793013736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 15.0 5 70.0 6 162.0 7 300.0 8 677.0 9 1322.0 10 2197.0 11 3589.0 12 5354.0 13 7306.0 14 9244.0 15 12453.0 16 17027.0 17 25055.0 18 24116.0 19 14804.0 20 12993.0 21 13644.0 22 14504.0 23 15917.0 24 18066.0 25 21351.0 26 25369.0 27 30554.0 28 36643.0 29 45012.0 30 53955.0 31 65704.0 32 80394.0 33 102093.0 34 137918.0 35 195727.0 36 297687.0 37 439618.0 38 292076.0 39 16701.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72736427919596 19.779202356329932 15.262985360552147 21.230448003921968 2 15.468853725717457 21.683154918970757 40.011394201116474 22.836597154195314 3 19.59126000056873 26.412091581136615 30.98884407692753 23.007804341367127 4 12.578776388312804 17.622720546921652 37.78472019980055 32.01378286496499 5 12.844216168194883 38.80014747743958 33.86578180202919 14.48985455233635 6 29.65100601382405 37.07037635183567 17.756421004945004 15.522196629395275 7 26.909827939718596 34.90758636108243 20.620268146147787 17.562317553051184 8 25.64053158556878 34.697548677851024 19.830077357016012 19.831842379564176 9 26.72033315781165 17.40944698901862 20.896837373764114 34.973382479405615 10-11 24.739904316166527 27.018499397440916 26.83557442168546 21.4060218647071 12-13 26.939980408249713 24.843869047093744 27.42565577941925 20.79049476523729 14-15 23.50585918826917 25.66106773967858 27.371266272050093 23.461806800002158 16-17 21.007119536732468 29.133349251495023 28.058897851979726 21.80063335979278 18-19 20.839889891497183 27.67426062983652 30.88450839848217 20.60134108018412 20-21 22.507273363750553 26.293908613015866 30.269303218126385 20.929514805107193 22-23 21.800161205392733 26.457908624782682 30.01489482917033 21.727035340654254 24-25 21.81971507121994 26.521150256234886 30.613186444126576 21.045948228418602 26-27 21.969535213066465 26.222970940612615 30.82114997620896 20.98634387011196 28-29 21.122515117908407 26.64012268867846 31.16622884301791 21.07113335039522 30-31 21.25557820667826 26.915931976030993 30.691437777562054 21.137052039728697 32-33 21.05223290060864 26.973957092301852 30.350420712735716 21.62338929435379 34-35 21.8310546324323 26.46329079289769 30.29646566404808 21.409188910621936 36-37 22.040876475111418 26.294841817839433 30.480102669323962 21.18417903772519 38-39 22.051501695573464 26.200042364819943 30.75600119249123 20.992454747115367 40-41 21.591426424567516 26.429323530385172 30.871711393230523 21.107538651816785 42-43 21.116213049084838 27.051918824435038 30.145548239055074 21.686319887425043 44-45 21.457468837708486 27.277298123239092 29.498880787917464 21.76635225113496 46-47 21.73475834048133 27.186398612875813 28.74733007275146 22.331512973891403 48-49 22.059435319601576 27.18779885157644 28.69347759843517 22.059288230386816 50-51 22.209196971212396 27.572503417289816 28.659025077121907 21.559274534375877 52-53 21.957591411008053 27.7480665648837 28.665584119503794 21.628757904604452 54-55 21.214149205200542 27.374224983501943 28.97578683234345 22.435838978954067 56-57 21.900081092980408 28.099698279201075 28.004239976387918 21.9959806514306 58-59 22.012859220268812 27.310864835023672 28.332718841591547 22.343557103115963 60-61 21.877447619262412 27.098716690621416 28.95988178129196 22.063953908824214 62-63 22.458229075069134 26.910215279270783 28.756257640873372 21.875298004786707 64-65 21.979320162412648 27.184310603031598 28.914682370903005 21.921686863652756 66-67 21.491529130045528 27.543589899479205 28.57510230057394 22.38977866990132 68-69 21.37634812435618 27.827512645905333 28.637919428042753 22.15821980169573 70-71 21.69166134708482 27.685457292828026 28.098913824735224 22.52396753535193 72-73 21.60676866535859 27.381039931674017 27.963056116930783 23.049135286036613 74-75 21.59605595905932 27.848697364331056 27.91772935732587 22.637517319283752 76-77 21.89699406114343 27.166710996257663 28.31407040847673 22.62222453412217 78-79 21.46232353078894 27.26966733682903 28.594098076762315 22.673911055619715 80-81 21.4043023447367 28.065454944813574 28.08224718968912 22.447995520760607 82-83 21.447597951397164 27.857424420263637 27.891155962295194 22.803821666044005 84-85 21.416725807222626 27.33938589427262 27.777430492993222 23.46645780551153 86-87 21.593029880842725 27.33626732138309 28.04455650830558 23.026146289468606 88-89 21.84748971242054 26.962519526209938 28.080118033846084 23.109872727523438 90-91 21.298323954151034 27.140759598095514 28.415796797345184 23.14511965040827 92-93 21.223440087780453 27.618337407593714 28.0748653925165 23.08335711210933 94-95 21.09420294296098 27.592300007967204 27.75385030244716 23.559646746624665 96-97 20.927333041124044 27.28408626253534 27.642753552843303 24.145827143497314 98-99 21.058336936921034 27.199046495596757 27.48017536479584 24.262441202686365 100-101 21.437270485333123 26.69851884900693 28.395640385756238 23.46857027990371 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1257.0 1 876.0 2 559.5 3 611.5 4 559.5 5 584.5 6 966.0 7 2085.5 8 3417.5 9 3995.0 10 3624.0 11 3120.5 12 3171.0 13 3387.5 14 3656.0 15 3925.0 16 4095.5 17 4235.0 18 4417.5 19 4696.0 20 5219.0 21 5941.5 22 6863.0 23 8175.5 24 9584.5 25 11218.5 26 13980.5 27 16919.5 28 19346.0 29 22023.5 30 25333.0 31 28897.5 32 32595.0 33 36655.0 34 41272.5 35 45029.0 36 49090.0 37 54007.0 38 56201.0 39 56070.5 40 56133.5 41 58186.0 42 60825.0 43 63810.5 44 67089.5 45 69076.5 46 70608.5 47 78100.5 48 110996.5 49 112508.5 50 81473.0 51 71081.0 52 64684.0 53 62130.0 54 59900.5 55 54799.5 56 49104.5 57 43417.0 58 39188.5 59 35363.0 60 30796.0 61 25794.5 62 20861.0 63 16080.5 64 11662.5 65 8677.0 66 5974.0 67 4333.5 68 3081.5 69 1851.0 70 1265.5 71 957.5 72 730.5 73 629.0 74 494.5 75 396.5 76 290.5 77 120.5 78 43.0 79 25.5 80 19.0 81 15.5 82 8.5 83 3.5 84 4.5 85 3.5 86 1.0 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3922272329251229 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.334828699658861E-4 16-17 0.0017159941440474124 18-19 4.6576983909858336E-4 20-21 0.0 22-23 0.0 24-25 7.354260617346053E-5 26-27 2.451420205782018E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011815845391869325 36-37 0.022847236317888407 38-39 0.010099851247821914 40-41 0.005466667058893899 42-43 0.005098954028026597 44-45 0.0014463379214113906 46-47 9.070254761393466E-4 48-49 0.0027210764284180395 50-51 2.941704246938421E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.9222723292512286E-4 60-61 0.0012011959008331888 62-63 0.0013482811131801098 64-65 7.109118596767852E-4 66-67 0.00274559063047586 68-69 0.0020101645687412547 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09158505888801619 78-79 0.0038487297230777678 80-81 9.805680823128071E-5 82-83 0.0 84-85 7.354260617346053E-5 86-87 0.09349716664852616 88-89 0.44272648916423246 90-91 0.39257043175393236 92-93 0.0 94-95 0.0010541106884862676 96-97 0.0 98-99 0.0 100-101 1.9611361646256143E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2039634.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.92341742312672 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95984882616075 34.96485290424324 2 14.403196952726354 13.805008395860238 3 5.258070330302496 7.559540978377329 4 2.4901476209335183 4.773455355328117 5 1.3149508058425436 3.150846817963454 6 0.7928066843851701 2.2796403402981356 7 0.5289810762810376 1.7745406649285704 8 0.3792994159020326 1.4541859389297007 9 0.27418441102937713 1.182586858260749 >10 1.401996969743658 12.373574084413772 >50 0.1204658324737702 3.9706103494012357 >100 0.06827356084683928 5.891515517694212 >500 0.004502770899821766 1.5095363304346423 >1k 0.002660728258985588 2.6367843016835133 >5k 3.070071068060294E-4 1.048092947294306 >10k+ 3.070071068060294E-4 1.6252282148886812 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11766 0.5768682028246245 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10786 0.5288203667912968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10587 0.5190637143722844 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9132 0.44772738638402776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6476 0.31750794505288693 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5763 0.2825506929184354 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4959 0.24313185600946052 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3778 0.18522931074888926 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3778 0.18522931074888926 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3540 0.17356055056936687 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2939 0.144094479695867 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2872 0.14080957662011911 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.12624814059777392 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2477 0.12144335699444116 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2379 0.1166385733911084 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2284 0.11198087500012258 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.902840411564036E-5 0.0 0.0 0.0 0.0 6 4.902840411564036E-5 0.0 0.0 0.0 0.0 7 9.805680823128071E-5 0.0 0.0 0.0 0.0 8 9.805680823128071E-5 0.0 0.0 0.0 0.0 9 9.805680823128071E-5 0.0 0.0 4.902840411564036E-5 0.0 10-11 9.805680823128071E-5 0.0 0.0 4.902840411564036E-5 0.0 12-13 1.4708521234692106E-4 0.0 0.0 9.805680823128071E-5 0.0 14-15 1.9611361646256143E-4 0.0 0.0 9.805680823128071E-5 0.0 16-17 2.2062781852038161E-4 0.0 0.0 1.2257101028910087E-4 0.0 18-19 2.69656222636022E-4 0.0 0.0 1.4708521234692106E-4 0.0 20-21 2.941704246938421E-4 0.0 0.0 1.9611361646256143E-4 0.0 22-23 2.941704246938421E-4 0.0 0.0 2.69656222636022E-4 0.0 24-25 4.412556370407632E-4 0.0 0.0 8.579970720237063E-4 0.0 26-27 4.412556370407632E-4 0.0 0.0 0.0017895367502208728 0.0 28-29 5.638266473298641E-4 0.0 0.0 0.004608669986870193 0.0 30-31 5.883408493876842E-4 0.0 0.0 0.0136053821420902 0.0 32-33 5.883408493876842E-4 0.0 0.0 0.03007892592494536 0.0 34-35 6.128550514455044E-4 0.0 0.0 0.05822122988732292 0.0 36-37 7.354260617346053E-4 0.0 0.0 0.10259193561197744 0.0 38-39 8.334828699658861E-4 0.0 0.0 0.1669417160137554 0.0 40-41 8.334828699658861E-4 0.0 0.0 0.23663559246413818 0.0 42-43 8.334828699658861E-4 0.0 0.0 0.30961437199026887 0.0 44-45 8.825112740815263E-4 0.0 0.0 0.3995324651383533 0.0 46-47 8.825112740815263E-4 0.0 0.0 0.5127635644434246 0.0 48-49 8.825112740815263E-4 0.0 0.0 0.640605128174957 0.0 50-51 8.825112740815263E-4 0.0 0.0 0.7746242708250598 0.0 52-53 8.825112740815263E-4 0.0 0.0 0.9047701695500271 0.0 54-55 8.825112740815263E-4 0.0 0.0 1.0504090439755367 0.0 56-57 8.825112740815263E-4 0.0 0.0 1.221052404500023 0.0 58-59 9.315396781971667E-4 0.0 0.0 1.4265304461486719 0.0 60-61 9.315396781971667E-4 0.0 0.0 1.6656910014247654 0.0 62-63 9.315396781971667E-4 0.0 0.0 1.8979875801246693 0.0 64-65 9.315396781971667E-4 0.0 0.0 2.1335935761023794 0.0 66-67 9.315396781971667E-4 0.0 0.0 2.397537989658929 0.0 68-69 9.315396781971667E-4 0.0 0.0 2.7001903282647772 0.0 70-71 9.56053880254987E-4 0.0 0.0 3.041305449899345 0.0 72-73 0.0010050822843706273 0.0 0.0 3.4071063730061377 0.0 74-75 0.0010541106884862676 0.0 0.0 3.7794525880623677 0.0 76-77 0.0010786248905440877 0.0 0.0 4.1633695064898895 0.0 78-79 0.0010786248905440877 0.0 0.0 4.6102634100039515 0.0 80-81 0.0010786248905440877 0.0 0.0 5.104763893914301 0.0 82-83 0.0010786248905440877 0.0 0.0 5.607157950887267 0.0 84-85 0.0011521674967175483 0.0 0.0 6.150098498063868 0.0 86-87 0.001225710102891009 0.0 0.0 6.718288673360025 0.0 88-89 0.001225710102891009 0.0 0.0 7.301702168134087 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 165 8.497227E-8 25.88371 6 GAGTACT 10745 0.0 22.591192 12-13 GTACATG 13305 0.0 22.414768 1 TACATGG 13490 0.0 21.915133 2 AGTACTT 10905 0.0 21.454638 12-13 ACATGGG 13235 0.0 21.333445 3 GTACTTT 11445 0.0 21.271667 14-15 CTTTGCG 500 0.0 19.930458 2 GTATACG 120 0.004684114 19.850138 1 CATGGGA 9045 0.0 19.464056 4 GTAAGCG 150 3.767127E-9 18.982319 94-95 ACCGTCG 125 0.006066953 18.981388 8 TATAACG 150 7.7749335E-4 18.981388 2 ACTTTTT 13285 0.0 18.467707 16-17 GCACCGT 415 1.8189894E-12 18.295315 6 TACTTTT 12845 0.0 18.048054 14-15 ATGGGAG 3045 0.0 17.921673 5 TAGGTCG 135 0.00945603 17.575361 5 ATCACGC 135 0.00945603 17.575361 3 CATGGGG 4125 0.0 17.485886 4 >>END_MODULE