FastQCFastQC Report
Fri 27 May 2016
SRR938944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060355
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124320.6033911631733366No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA105710.5130669229331838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103500.502340616058883No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA86540.4200247044805386No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT64040.31082022272860743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.30999512219981507No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46260.22452441448197036No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA36890.17904681474794393No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT36060.17501838275442824No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG36040.1749213121039821No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC28270.13720936440564854No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG28080.13628719322641003No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26480.12852154119071713No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.11905715277221644No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG21620.10493337313230001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135900.024.3485471
TACATGG137350.023.8420542
TATAACG1000.00169055823.661172
GAGTACT103150.023.32827812-13
ACATGGG138550.022.9865993
CATGGGG39950.021.795524
GTACTTT112200.021.51146314-15
AGTACTT105550.020.91483112-13
TCGCGGA1803.6379788E-1219.81657482-83
CATGGGA96150.019.6868824
TAGGACG1456.305411E-419.581664
TTTGCGT5150.019.2964883
CTTTGCG5250.018.9289342
TACCTGG16050.018.8699672
ACTTTTT132600.018.56093816-17
GTCGGGA5650.018.42642
CGCGGAA1951.4551915E-1118.29222182-83
TACTTTT126450.017.42181614-15
ATGGGAT37300.017.0005195
GTATAGA6450.016.9046121
Produced by FastQC (version 0.11.5)