FastQCFastQC Report
Fri 27 May 2016
SRR938944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060355
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119160.5783469353582271No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA109290.5304425693630466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107060.5196191918382997No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA92810.45045635339541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65200.3164503204544848No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT57500.2790781200327128No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49720.2413176370091562No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA38750.1880743852394369No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38680.18773463796287534No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT34800.1689029317763201No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC29630.1438101686359875No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG29030.14089804912260268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.13075416615097882No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26220.12725962273491706No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG24160.11726134573896245No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT21870.10614675626287703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG4850.025.438812
GAGTACT101950.022.80730412-13
TTTGCGT5100.022.33093
GTACTTT108600.021.30148914-15
GTACATG139450.021.1831931
AGTACTT104400.020.97666212-13
TACATGG142800.020.5696832
GTATAGG5950.020.018951
ACATGGG140900.019.8703783
TACCTGG16200.018.7469272
GTGTAGC9350.018.3446391
ACTTTTT128200.018.22964116-17
CATGGGG42650.018.0244124
TACTTTT121150.017.8414514-15
TCGCGCT2002.3646862E-1117.795860-61
ACCGTCC1600.001199554417.7945048
CGCGGAA1901.9281288E-1017.59375682-83
CATGGGA94250.017.5211684
TCGCGGA2104.3655746E-1117.0551782-83
ATGGGAT37650.016.6369655
Produced by FastQC (version 0.11.5)