##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938944_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2060355 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13796748618563 33.0 31.0 34.0 30.0 34.0 2 32.369215013917504 34.0 31.0 34.0 30.0 34.0 3 32.44916240162496 34.0 31.0 34.0 30.0 34.0 4 36.03081362192438 37.0 35.0 37.0 35.0 37.0 5 35.9592720671923 37.0 35.0 37.0 35.0 37.0 6 36.00660517240961 37.0 35.0 37.0 35.0 37.0 7 35.94945045878016 37.0 35.0 37.0 35.0 37.0 8 35.922626925942374 37.0 35.0 37.0 35.0 37.0 9 37.58187254138243 39.0 37.0 39.0 35.0 39.0 10-11 37.50969032035741 39.0 37.0 39.0 34.5 39.0 12-13 37.43204205100577 39.0 37.0 39.0 34.0 39.0 14-15 38.633787866654046 40.0 38.0 41.0 34.0 41.0 16-17 38.63203234394072 40.0 38.0 41.0 34.0 41.0 18-19 38.78432187656982 40.0 38.0 41.0 34.0 41.0 20-21 38.80010507897911 40.0 38.0 41.0 34.0 41.0 22-23 38.74341848856143 40.0 38.0 41.0 34.0 41.0 24-25 38.678129254424604 40.0 38.0 41.0 34.0 41.0 26-27 38.50257649773947 40.0 38.0 41.0 34.0 41.0 28-29 38.31361124660556 40.0 38.0 41.0 34.0 41.0 30-31 38.12063600690172 40.0 37.5 41.0 33.0 41.0 32-33 37.923631364497865 40.0 37.0 41.0 33.0 41.0 34-35 37.872292396213275 40.0 37.0 41.0 33.0 41.0 36-37 37.73421643357577 40.0 37.0 41.0 33.0 41.0 38-39 37.5788745143434 40.0 37.0 41.0 33.0 41.0 40-41 37.38884949438325 40.0 36.5 41.0 32.0 41.0 42-43 37.20105442994047 40.0 36.0 41.0 31.5 41.0 44-45 36.90536339611377 40.0 36.0 41.0 31.0 41.0 46-47 36.48304394145669 40.0 36.0 41.0 30.0 41.0 48-49 36.116935431030086 39.0 35.5 41.0 29.5 41.0 50-51 35.823967228948405 39.0 35.0 41.0 28.0 41.0 52-53 35.56270982427786 39.0 35.0 40.5 28.0 41.0 54-55 35.32164262954685 39.0 35.0 40.0 27.0 41.0 56-57 35.06769440217826 38.0 34.5 40.0 26.5 41.0 58-59 34.77812973977785 38.0 34.0 40.0 26.0 41.0 60-61 34.37341137813629 38.0 34.0 40.0 25.5 41.0 62-63 34.052676601847736 37.0 34.0 40.0 25.0 41.0 64-65 33.78177450002548 37.0 33.0 39.5 24.0 41.0 66-67 33.33830553472581 36.0 33.0 39.0 22.5 41.0 68-69 32.94240264420452 36.0 32.5 39.0 22.0 40.0 70-71 32.477614051947356 35.0 32.0 38.5 21.5 40.0 72-73 31.95802859216009 35.0 31.5 37.5 19.5 39.5 74-75 31.494276714449693 35.0 31.0 37.0 18.0 39.0 76-77 29.524920948089044 33.0 28.5 35.0 13.0 37.5 78-79 30.468528967095477 34.0 30.0 36.0 12.5 38.0 80-81 30.477814988193785 34.0 31.0 35.5 11.5 37.0 82-83 30.161672867054463 34.0 30.0 35.0 7.5 37.0 84-85 29.7766443161494 34.0 30.0 35.0 4.5 36.5 86-87 29.204281543714554 34.0 29.0 35.0 2.0 36.0 88-89 28.622483018703086 34.0 29.0 35.0 2.0 35.5 90-91 28.155850326764075 33.5 28.0 35.0 2.0 35.0 92-93 27.821131795248874 33.0 27.0 35.0 2.0 35.0 94-95 27.45238854469254 33.0 27.0 35.0 2.0 35.0 96-97 26.832837302309553 33.0 25.5 35.0 2.0 35.0 98-99 25.96265400865385 33.0 24.5 34.5 2.0 35.0 100-101 21.799001385683535 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12992102126528948 1101 2 0.1667272308066643 1101 3 0.21635309273430892 1101 4 0.14150336206146363 1101 5 0.24815990542902 1101 6 0.13724147649389806 1101 7 0.194774394886565 1101 8 0.16091424806182175 1101 9 0.19427679842124235 1101 10-11 0.2608298574148833 1101 12-13 0.22340180041560131 1101 14-15 0.2487114173344267 1101 16-17 0.25631796688987407 1101 18-19 0.35221997422979 1101 20-21 0.24881635652864276 1101 22-23 0.20799874769294036 1101 24-25 0.2133125440982795 1101 26-27 0.1348183546934294 1101 28-29 0.2854546005698566 1101 30-31 0.2870948886302003 1101 32-33 0.3661499775526025 1101 34-35 0.5095396315800116 1101 36-37 0.4921910780642733 1101 38-39 0.4509713296270377 1101 40-41 0.5492662129711405 1101 42-43 0.5547022927984315 1101 44-45 0.6270770155056411 1101 46-47 0.6046218704204449 1101 48-49 0.35940405259685093 1101 50-51 0.5226908265719175 1101 52-53 0.4921175006676535 1101 54-55 0.5792136393199385 1101 56-57 0.42341789131348406 1101 58-59 0.5100252906409963 1101 60-61 0.6759067084530415 1101 62-63 0.5121803509631562 1101 64-65 0.41182370752625275 1101 66-67 0.610136990774933 1101 68-69 0.540241002497929 1101 70-71 0.6785057436692483 1101 72-73 0.6913004534433576 1101 74-75 0.5595625967920093 1101 76-77 0.3975442675619725 1101 78-79 0.6241793531698896 1101 80-81 0.7294960010703591 1101 82-83 0.7687491654171374 1101 84-85 0.7389413874580022 1101 86-87 0.9263817321334287 1101 88-89 1.0387853520264976 1101 90-91 1.111631994823913 1101 92-93 1.0621943461386678 1101 94-95 0.9913381784814668 1101 96-97 0.9041403441642579 1101 98-99 0.8547932607712276 1101 100-101 1.061818718083373 1102 1 0.14047249534142736 1102 2 0.21053806932872732 1102 3 0.21045790431404043 1102 4 0.16098538706376075 1102 5 0.11819636732160177 1102 6 0.16687469126393495 1102 7 0.09869855090308022 1102 8 0.11737812937879255 1102 9 0.23019305630580078 1102 10-11 0.2575659422081955 1102 12-13 0.2929480281799073 1102 14-15 0.3478794449170053 1102 16-17 0.35042726835063576 1102 18-19 0.3260773248752926 1102 20-21 0.23954995235512655 1102 22-23 0.2905649962679746 1102 24-25 0.33406396246945746 1102 26-27 0.3326185412898397 1102 28-29 0.3210001261877977 1102 30-31 0.21091695439147884 1102 32-33 0.3082516933974162 1102 34-35 0.4633534684294034 1102 36-37 0.49560427547343266 1102 38-39 0.5691781015852229 1102 40-41 0.48274393276096816 1102 42-43 0.2698756554758077 1102 44-45 0.335769095406242 1102 46-47 0.5745334527407664 1102 48-49 0.5252819460968752 1102 50-51 0.5188328265424929 1102 52-53 0.5533919470551822 1102 54-55 0.5930413313841072 1102 56-57 0.40824970341424205 1102 58-59 0.39729985168536786 1102 60-61 0.3923650142002444 1102 62-63 0.6146833201189352 1102 64-65 0.561613494847947 1102 66-67 0.6843472409957485 1102 68-69 0.5728639199668137 1102 70-71 0.5455859867214699 1102 72-73 0.6760970060264562 1102 74-75 0.6899468392882007 1102 76-77 0.578178142369886 1102 78-79 0.5380335025555887 1102 80-81 0.7105769868554432 1102 82-83 0.6006727522080588 1102 84-85 0.7196525288060478 1102 86-87 0.6309017739983993 1102 88-89 0.8314634468355351 1102 90-91 0.8094120150055453 1102 92-93 0.8158898733547026 1102 94-95 0.8950763976170819 1102 96-97 0.7686358616395346 1102 98-99 0.8735898813367093 1102 100-101 1.3929693858073122 1103 1 0.027554893363294752 1103 2 0.1015346563988544 1103 3 0.1348869972256992 1103 4 0.08071915995896717 1103 5 -0.021182297210515344 1103 6 -0.011624528232609066 1103 7 0.04236073485188996 1103 8 0.018737503088466667 1103 9 0.12503681379676834 1103 10-11 0.1158094969364285 1103 12-13 0.03317830619175055 1103 14-15 0.1432374385951718 1103 16-17 0.12129560134636108 1103 18-19 0.07938263588815886 1103 20-21 0.1403327650641657 1103 22-23 0.12296270438152135 1103 24-25 0.1609030151021713 1103 26-27 0.13370687861793584 1103 28-29 0.35165537508920863 1103 30-31 0.12181594025155817 1103 32-33 0.24609053593803054 1103 34-35 0.20495009812036358 1103 36-37 0.0887295448343437 1103 38-39 0.19427627634419764 1103 40-41 0.21729494686324102 1103 42-43 0.17424270972097844 1103 44-45 0.3286574652145262 1103 46-47 0.4343584713716879 1103 48-49 0.3056314068228332 1103 50-51 0.14998953542085758 1103 52-53 0.22582924385005754 1103 54-55 0.3363328797327796 1103 56-57 0.2629043729935816 1103 58-59 0.22089264248911178 1103 60-61 0.20366842108087013 1103 62-63 0.2623726709594507 1103 64-65 0.25895084729087614 1103 66-67 0.25183073169735337 1103 68-69 0.30467482603103946 1103 70-71 0.32122556907744837 1103 72-73 0.14501040315047575 1103 74-75 0.014631381102930163 1103 76-77 0.09417049371215214 1103 78-79 0.13489713119508195 1103 80-81 0.124708303706214 1103 82-83 0.32420999536293493 1103 84-85 0.21942235621677142 1103 86-87 0.2213747874545824 1103 88-89 0.41892024554897844 1103 90-91 0.5078643365232658 1103 92-93 0.5810666431422042 1103 94-95 0.4452103470197777 1103 96-97 0.4118511543364889 1103 98-99 0.6242188398597115 1103 100-101 0.9245126942734743 1104 1 0.037080001142655306 1104 2 0.05827694633362057 1104 3 0.024924587025346057 1104 4 0.039209076224310024 1104 5 0.07585994913255689 1104 6 0.03097336877717538 1104 7 0.00912985767904928 1104 8 0.01965980645205434 1104 9 0.03589846977662603 1104 10-11 0.008478916988714502 1104 12-13 0.12700424221338835 1104 14-15 0.09030333627985954 1104 16-17 0.049437209548528926 1104 18-19 0.007259032393498899 1104 20-21 0.031167226949143867 1104 22-23 0.02175166167896947 1104 24-25 0.01409015708493655 1104 26-27 -0.0037003653228921962 1104 28-29 -0.08799139855607052 1104 30-31 -0.0635100103666204 1104 32-33 -0.04990897322241494 1104 34-35 0.02478711662629962 1104 36-37 -0.06544034008060606 1104 38-39 0.16851895098436387 1104 40-41 0.01678983238431897 1104 42-43 0.16873127914632846 1104 44-45 0.1954040499954388 1104 46-47 0.16614310364033713 1104 48-49 0.07032863748823814 1104 50-51 0.019098285576099272 1104 52-53 0.09607442117821563 1104 54-55 0.13847443944474946 1104 56-57 0.04810974135333623 1104 58-59 0.03883087389323947 1104 60-61 0.17323649216552184 1104 62-63 0.04974389361010623 1104 64-65 0.10554789918577256 1104 66-67 0.04845999493827691 1104 68-69 0.20521561544157407 1104 70-71 -0.10870127009886943 1104 72-73 -0.13668536340781046 1104 74-75 -0.12647497225108495 1104 76-77 -0.1614527611973564 1104 78-79 0.053148099638352875 1104 80-81 0.17826585142823603 1104 82-83 0.1681585175746605 1104 84-85 0.16169663063269724 1104 86-87 0.10367559293615258 1104 88-89 0.19512276535697382 1104 90-91 0.29528912105113747 1104 92-93 0.11823388534134338 1104 94-95 0.21367793800198598 1104 96-97 0.21242556526716783 1104 98-99 0.517607448720451 1104 100-101 1.2215746583145304 1105 1 -0.033458797984728506 1105 2 -0.02867164155801305 1105 3 -0.08853262249230909 1105 4 -0.10480990153889991 1105 5 0.006957422095091204 1105 6 -0.0433463074731506 1105 7 -0.00790701987256881 1105 8 0.04205493804173699 1105 9 -0.036322462437446745 1105 10-11 0.03837304567080224 1105 12-13 -0.007427735549335068 1105 14-15 0.04951927254176525 1105 16-17 -0.00650149988440063 1105 18-19 -0.03142339082433665 1105 20-21 -0.0782028022619059 1105 22-23 -0.08728877178950967 1105 24-25 0.051277511808159204 1105 26-27 -0.013456326890413095 1105 28-29 0.0011809649314287185 1105 30-31 -0.0673182834007875 1105 32-33 -0.10068168274320755 1105 34-35 0.014141693095169217 1105 36-37 0.08310391119518101 1105 38-39 0.0021537197990824097 1105 40-41 -0.03017919020198434 1105 42-43 -0.02712450394950139 1105 44-45 -0.02400594103995246 1105 46-47 -0.05713140049366672 1105 48-49 -0.022816374500798986 1105 50-51 0.048449444193835234 1105 52-53 0.14441142902334292 1105 54-55 0.10459342121475856 1105 56-57 0.13178638849930735 1105 58-59 0.023700486251961195 1105 60-61 0.007513797682314305 1105 62-63 0.004969078345446576 1105 64-65 0.013915696655892873 1105 66-67 0.11479201310694975 1105 68-69 0.0014459139194187287 1105 70-71 0.05747762061534445 1105 72-73 0.12696842082440796 1105 74-75 0.08379419800762733 1105 76-77 0.004386878827610019 1105 78-79 0.0016457175216295639 1105 80-81 -0.06253129476262131 1105 82-83 -0.025703760403803955 1105 84-85 -0.15619140288155364 1105 86-87 -0.10638454772676553 1105 88-89 0.01597607437271975 1105 90-91 0.14842808904353788 1105 92-93 0.15502912854221051 1105 94-95 0.31153215023799063 1105 96-97 0.18057848224379924 1105 98-99 0.1911261505935613 1105 100-101 0.9415950833542475 1106 1 -0.06809311773312743 1106 2 -0.020565599971362758 1106 3 0.015090431672660998 1106 4 0.017717236021574934 1106 5 -0.03771843280154741 1106 6 -0.004820734901556989 1106 7 0.03836672586537304 1106 8 0.03503537639597454 1106 9 -0.05558799055324215 1106 10-11 0.010532146197981263 1106 12-13 0.08624176044747145 1106 14-15 -0.06700373415378635 1106 16-17 -0.03053697773410846 1106 18-19 -0.08326436277683769 1106 20-21 -0.11694712518367112 1106 22-23 -0.04850916270771677 1106 24-25 0.03483892963468094 1106 26-27 -0.04108550940306799 1106 28-29 -0.06548567789324267 1106 30-31 -0.1917592183028347 1106 32-33 -0.10170128587403582 1106 34-35 -0.19308403032476207 1106 36-37 -0.2001297506570765 1106 38-39 -0.03574921894200145 1106 40-41 -0.039264780359431484 1106 42-43 0.026506286944474766 1106 44-45 -0.12896092853601004 1106 46-47 -0.18704288230019728 1106 48-49 -0.2994800156176609 1106 50-51 -0.2667355971446099 1106 52-53 -0.1348759049856696 1106 54-55 -0.14722506773173194 1106 56-57 -0.33354603216226764 1106 58-59 -0.10170569202087876 1106 60-61 -0.09332832560037474 1106 62-63 -0.10182686918212625 1106 64-65 -0.2348551886381003 1106 66-67 0.057228484309746364 1106 68-69 -0.08309960629990343 1106 70-71 -0.09339980074837939 1106 72-73 -0.055788496395454246 1106 74-75 -0.0025037092131405814 1106 76-77 -0.25691154695665475 1106 78-79 0.03271792325711331 1106 80-81 0.036053229538218545 1106 82-83 0.00991809498540519 1106 84-85 -0.04284295848860964 1106 86-87 0.11529944134501591 1106 88-89 0.1834843636400727 1106 90-91 0.39335719366438227 1106 92-93 0.25565107095471973 1106 94-95 0.3456248989559434 1106 96-97 0.028423725729030735 1106 98-99 0.2645950352338069 1106 100-101 0.6720683065232365 1107 1 -0.17365564547960233 1107 2 -0.1536312865471956 1107 3 -0.13459468141912367 1107 4 -0.14563123692632018 1107 5 -0.13921503492564824 1107 6 -0.025748690825928122 1107 7 -0.08421079351447247 1107 8 -0.08857729990084806 1107 9 -0.09068514589935006 1107 10-11 -0.09664785869678383 1107 12-13 -0.0761309614958634 1107 14-15 -0.08475294017775781 1107 16-17 -0.1109274061965877 1107 18-19 -0.10571979767080819 1107 20-21 -0.12160390841398794 1107 22-23 -0.11630980642591027 1107 24-25 -0.18595968503367288 1107 26-27 -0.1743375953687547 1107 28-29 -0.17015142536902061 1107 30-31 -0.27727171702222364 1107 32-33 -0.284431026679286 1107 34-35 -0.49024378740793395 1107 36-37 -0.4395167243278024 1107 38-39 -0.3576673682664051 1107 40-41 -0.49085358615118935 1107 42-43 -0.43689895955804303 1107 44-45 -0.5101903676872794 1107 46-47 -0.5595780705208924 1107 48-49 -0.3980120137052907 1107 50-51 -0.383955912686055 1107 52-53 -0.3726222794463965 1107 54-55 -0.4937536281023682 1107 56-57 -0.47289309359722864 1107 58-59 -0.2898289205152267 1107 60-61 -0.5294908032609058 1107 62-63 -0.6202234929342509 1107 64-65 -0.6210587168512873 1107 66-67 -0.5198433947099304 1107 68-69 -0.5299256231014624 1107 70-71 -0.6318795358620406 1107 72-73 -0.6020724609255019 1107 74-75 -0.7685859924491467 1107 76-77 -0.8767827179432324 1107 78-79 -0.6465997126863847 1107 80-81 -0.6094542605868796 1107 82-83 -0.5150220722333465 1107 84-85 -0.6916700652285819 1107 86-87 -0.6410456081148546 1107 88-89 -0.5455023066692064 1107 90-91 -0.3461112408084084 1107 92-93 -0.654117039666918 1107 94-95 -0.7303605988869428 1107 96-97 -0.6276640641149065 1107 98-99 -0.5355243457494723 1107 100-101 0.3073206286896806 1108 1 0.006614917042625734 1108 2 -0.037917980137216034 1108 3 0.0760578753436505 1108 4 0.01272322588770436 1108 5 0.0337412375662538 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-1.0737730433143824 2316 58-59 -0.8274818142727653 2316 60-61 -0.8481838734074714 2316 62-63 -0.8676956328482461 2316 64-65 -0.8432949688840523 2316 66-67 -0.9708606495062568 2316 68-69 -0.8763762057439024 2316 70-71 -1.0167636932752657 2316 72-73 -1.10655701192168 2316 74-75 -1.3864657077125813 2316 76-77 -1.2463859174882934 2316 78-79 -1.3780017958326525 2316 80-81 -1.5247996308741136 2316 82-83 -1.555672668843247 2316 84-85 -1.6161528952346664 2316 86-87 -1.741704510372589 2316 88-89 -1.7482025151833689 2316 90-91 -2.09427738868386 2316 92-93 -2.498751853051729 2316 94-95 -2.646044555268574 2316 96-97 -2.8226432002277626 2316 98-99 -3.1809436437850493 2316 100-101 -3.7334022993878975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 21.0 5 51.0 6 153.0 7 336.0 8 679.0 9 1226.0 10 2277.0 11 3538.0 12 5127.0 13 7313.0 14 8923.0 15 12308.0 16 16535.0 17 24184.0 18 22986.0 19 13904.0 20 11811.0 21 12273.0 22 12868.0 23 14050.0 24 16339.0 25 19315.0 26 23139.0 27 27763.0 28 33776.0 29 41184.0 30 50237.0 31 60727.0 32 75268.0 33 95785.0 34 128904.0 35 184098.0 36 283831.0 37 451683.0 38 366826.0 39 30899.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.695180301312796 19.804634996690957 15.274660153200315 21.225524548795928 2 15.425302921098549 21.66330559539497 40.02247185557828 22.888919627928196 3 19.607106542319165 26.450490328123067 30.897976319614823 23.044426809942948 4 12.596130278519963 17.61007205069029 37.76087130615841 32.03292636463134 5 12.840228214206844 38.888778335667084 33.758472488830755 14.512520961295316 6 29.664790776346795 37.01294194447073 17.816055970937047 15.506211308245424 7 26.900946681518477 34.93485345971932 20.60407065772646 17.560129201035743 8 25.719257118312132 34.68256683920975 19.77600947409548 19.82216656838263 9 26.73427637470242 17.41088307597477 20.907610581671605 34.947229967651204 10-11 24.699238723423875 27.052740911153663 26.827318593155063 21.4207017722674 12-13 26.960475257904587 24.830939328416704 27.393774373833637 20.814811039845075 14-15 23.499370852449427 25.62539207965724 27.353515399857546 23.521721668035784 16-17 21.067004805125467 29.17900305780712 27.975222055040526 21.778770082026888 18-19 20.890089329266072 27.703720960708228 30.807385135085948 20.598804574939756 20-21 22.54727947368293 26.285688631328096 30.218651640129977 20.948380254858993 22-23 21.833907263554096 26.443064423363932 30.00499913849798 21.718029174583993 24-25 21.803645488277507 26.527443086264263 30.564441564681815 21.10446986077642 26-27 21.958060625474737 26.3073596540402 30.761300843786625 20.973278876698433 28-29 21.190425921746495 26.597940646150786 31.118739246392007 21.092894185710716 30-31 21.219789793506457 26.945210898121925 30.68975977440781 21.145239533963807 32-33 21.0655202622849 26.993673420357172 30.342222578147943 21.59858373920999 34-35 21.793777235032078 26.484342617067565 30.25569988147392 21.466180266426445 36-37 22.026219708126273 26.306544102871264 30.478092967402286 21.18914322160017 38-39 22.027998844707863 26.269474317807273 30.73996946727926 20.962557370205598 40-41 21.597022767287815 26.451455550823237 30.841854533496903 21.109667148392045 42-43 21.1122767754763 27.110020353421994 30.127408490291536 21.650294380810173 44-45 21.43181290909391 27.303617936016572 29.43272634790467 21.831842806984845 46-47 21.72242470983629 27.19621539203012 28.768645841195077 22.31271405693851 48-49 22.047435179048595 27.173291314551882 28.73841488466636 22.040858621733168 50-51 22.183048517743924 27.553815173134126 28.659434199905938 21.60370210921601 52-53 21.965777742185207 27.722625469882615 28.719953600229086 21.59164318770309 54-55 21.20835972441642 27.36739542457489 29.025532007833597 22.398712843175083 56-57 21.918819815031874 28.10428299977431 27.97457234311563 22.002324842078185 58-59 21.949552733650286 27.394696870603752 28.327038689309752 22.328711706436213 60-61 21.927463307688313 27.127071715941636 28.842591087065095 22.102873889304952 62-63 22.449796272972375 26.958461041907828 28.719031429049846 21.872711256069948 64-65 21.978237187428636 27.230477159336314 28.88665248298893 21.904633170246115 66-67 21.471305785340576 27.512893024601283 28.591754822492337 22.424046367565804 68-69 21.38612131382663 27.86068166678194 28.647890434362665 22.105306585028764 70-71 21.65787489482999 27.697490892950704 28.10385300199553 22.540781210223777 72-73 21.618847237490627 27.42396334612239 27.94809632320644 23.009093093180542 74-75 21.592371217581434 27.87332765470028 27.898735897454564 22.635565230263715 76-77 21.8870739456084 27.193590125196664 28.324211370359798 22.595124558835142 78-79 21.502189187969243 27.274573823029353 28.574375361891867 22.64886162710953 80-81 21.368890312591763 28.101613556887038 28.11937748591869 22.41011864460251 82-83 21.47129984881246 27.8361495955794 27.89630913119341 22.796241424414728 84-85 21.425021416212257 27.36666739469655 27.81860407551126 23.38970711357994 86-87 21.58609580231743 27.33410360680472 28.07787766164806 23.00192292922979 88-89 21.859976803856078 26.96544418080512 28.063117107375525 23.111461907963278 90-91 21.2786210745788 27.229549626421708 28.407145546258107 23.084683752741384 92-93 21.201589046547802 27.73461369521272 28.086567606067884 22.97722965217159 94-95 21.10007741384373 27.568331670998447 27.774582535533927 23.557008379623902 96-97 20.93868676943417 27.344502490236472 27.63828802582478 24.078522714504576 98-99 21.06714619567987 27.28012890982379 27.487447551514183 24.165277342982154 100-101 21.416316241108316 26.96056304942068 28.16564839406082 23.457472315410183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1339.0 1 904.5 2 568.5 3 660.5 4 589.5 5 603.5 6 1006.5 7 2108.5 8 3450.0 9 4139.5 10 3775.5 11 3212.0 12 3201.5 13 3434.5 14 3728.5 15 3893.5 16 4062.5 17 4330.0 18 4517.0 19 4797.5 20 5247.0 21 5894.5 22 6837.5 23 8142.5 24 9542.0 25 11184.0 26 13873.0 27 16882.5 28 19378.0 29 22004.0 30 25341.0 31 28809.5 32 32768.0 33 37371.0 34 41921.0 35 45428.5 36 49841.5 37 54715.0 38 56923.0 39 57125.0 40 56919.0 41 58837.0 42 61436.0 43 64408.5 44 68022.5 45 70007.5 46 71212.5 47 78839.5 48 113108.5 49 114154.0 50 81993.5 51 71987.5 52 65238.0 53 62448.5 54 60489.5 55 55339.0 56 49333.0 57 43888.5 58 39938.5 59 35977.0 60 30934.5 61 25810.5 62 20994.0 63 16037.5 64 11578.5 65 8610.0 66 5850.5 67 4231.0 68 3034.5 69 1823.0 70 1264.0 71 951.0 72 732.0 73 620.5 74 495.5 75 389.5 76 274.0 77 122.5 78 56.5 79 34.5 80 20.0 81 10.5 82 5.5 83 5.0 84 2.5 85 2.0 86 1.5 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4077452671990992 2 0.0 3 0.0 4 0.0 5 4.853532522308049E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013347214436347135 16-17 0.002669442887269427 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002378230935930944 36-37 0.007231763458238992 38-39 0.013104537810231732 40-41 0.0025481045742117257 42-43 6.552268905115865E-4 44-45 4.3681792700772437E-4 46-47 4.610855896192646E-4 48-49 3.1547961395002316E-4 50-51 7.765652035692878E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.6694428872694266E-4 60-61 0.0022326249602617025 62-63 0.0 64-65 2.6694428872694266E-4 66-67 0.0017230040454193573 68-69 0.0018200746958655183 70-71 2.4267662611540245E-5 72-73 0.0 74-75 0.0 76-77 0.12582783064083616 78-79 9.707065044616098E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14934319571141866 88-89 0.44419529644163264 90-91 0.34479495038476377 92-93 0.0 94-95 0.0 96-97 4.1255026439618417E-4 98-99 0.0 100-101 1.4560597566924148E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2060355.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.57750146742431 #Duplication Level Percentage of deduplicated Percentage of total 1 72.49431826622606 34.490985336912956 2 14.658000555661706 13.947820858930026 3 5.37671059531095 7.674313687149676 4 2.489841838631608 4.738418149245991 5 1.370089042525007 3.2592706715617745 6 0.803322030133285 2.2932033040488387 7 0.5266978933236809 1.75412788547477 8 0.3788916159080024 1.4421373129486204 9 0.2667592795536721 1.14225660129725 >10 1.4381049040554383 12.536918923897053 >50 0.12014775791500194 3.937738954802351 >100 0.06935516718216005 5.963558368346944 >500 0.004186884164613347 1.3434925687702666 >1k 0.0029614546530191965 2.7985865562612506 >5k 3.0635737789853754E-4 1.0470710382456245 >10k+ 3.0635737789853754E-4 1.6300997821065817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11916 0.5783469353582271 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 10929 0.5304425693630466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10706 0.5196191918382997 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9281 0.45045635339541 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6520 0.3164503204544848 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5750 0.2790781200327128 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4972 0.2413176370091562 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3875 0.1880743852394369 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3868 0.18773463796287534 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3480 0.1689029317763201 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2963 0.1438101686359875 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2903 0.14089804912260268 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.13075416615097882 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2622 0.12725962273491706 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2416 0.11726134573896245 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2187 0.10614675626287703 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.853532522308049E-5 0.0 0.0 0.0 0.0 8 4.853532522308049E-5 0.0 0.0 0.0 0.0 9 4.853532522308049E-5 0.0 0.0 0.0 0.0 10-11 4.853532522308049E-5 0.0 0.0 0.0 0.0 12-13 4.853532522308049E-5 0.0 0.0 4.853532522308049E-5 0.0 14-15 9.707065044616098E-5 0.0 0.0 4.853532522308049E-5 0.0 16-17 9.707065044616098E-5 0.0 0.0 4.853532522308049E-5 0.0 18-19 9.707065044616098E-5 0.0 0.0 7.280298783462073E-5 0.0 20-21 9.707065044616098E-5 0.0 0.0 1.698736382807817E-4 0.0 22-23 9.707065044616098E-5 0.0 0.0 2.4267662611540244E-4 0.0 24-25 2.912119513384829E-4 0.0 0.0 5.096209148423451E-4 0.0 26-27 2.912119513384829E-4 0.0 0.0 0.0011648478053539317 0.0 28-29 4.125502643961841E-4 0.0 0.0 0.004101234981350301 0.0 30-31 4.8535325223080487E-4 0.0 0.0 0.013444285086793295 0.0 32-33 4.8535325223080487E-4 0.0 0.0 0.031256749443663835 0.0 34-35 4.8535325223080487E-4 0.0 0.0 0.058582137544258145 0.0 36-37 5.338885774538853E-4 0.0 0.0 0.10716599809256172 0.0 38-39 5.824239026769658E-4 0.0 0.0 0.1761346952345591 0.0 40-41 6.06691565288506E-4 0.0 0.0 0.2520682115460685 0.0 42-43 6.552268905115866E-4 0.0 0.0 0.32877829306114725 0.0 44-45 7.280298783462073E-4 0.0 0.0 0.4193937452526385 0.0 46-47 8.493681914039085E-4 0.0 0.0 0.5310249932657236 0.0 48-49 9.221711792385292E-4 0.0 0.0 0.661487947465364 0.0 50-51 9.221711792385292E-4 0.0 0.0 0.7977266053665509 0.0 52-53 9.221711792385292E-4 0.0 0.0 0.9332615010520031 0.0 54-55 9.707065044616097E-4 0.0 0.0 1.0807118190797218 0.0 56-57 9.707065044616097E-4 0.0 0.0 1.2562640904116038 0.0 58-59 9.707065044616097E-4 0.0 0.0 1.4647475799073462 0.0 60-61 9.9497416707315E-4 0.0 0.0 1.7143404898670376 0.0 62-63 0.0010677771549077706 0.0 0.0 1.9471159096369317 0.0 64-65 0.0010677771549077706 0.0 0.0 2.178629410951025 0.0 66-67 0.0010677771549077706 0.0 0.0 2.44419044290911 0.0 68-69 0.0010677771549077706 0.0 0.0 2.7532633939296867 0.0 70-71 0.0011163124801308513 0.0 0.0 3.1055570520614166 0.0 72-73 0.0011648478053539317 0.0 0.0 3.469669061884966 0.0 74-75 0.0011648478053539317 0.0 0.0 3.84596343833951 0.0 76-77 0.0011648478053539317 0.0 0.0 4.239026769658627 0.0 78-79 0.0011648478053539317 0.0 0.0 4.694360923238956 0.0 80-81 0.0012619184558000927 0.0 0.0 5.1993224468598855 0.0 82-83 0.0013104537810231733 0.0 0.0 5.71617512516047 0.0 84-85 0.001407524431469334 0.0 0.0 6.263774931989875 0.0 86-87 0.001504595081915495 0.0 0.0 6.844208886332694 0.0 88-89 0.001504595081915495 0.0 0.0 7.432796775312992 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 485 0.0 25.43881 2 GAGTACT 10195 0.0 22.807304 12-13 TTTGCGT 510 0.0 22.3309 3 GTACTTT 10860 0.0 21.301489 14-15 GTACATG 13945 0.0 21.183193 1 AGTACTT 10440 0.0 20.976662 12-13 TACATGG 14280 0.0 20.569683 2 GTATAGG 595 0.0 20.01895 1 ACATGGG 14090 0.0 19.870378 3 TACCTGG 1620 0.0 18.746927 2 GTGTAGC 935 0.0 18.344639 1 ACTTTTT 12820 0.0 18.229641 16-17 CATGGGG 4265 0.0 18.024412 4 TACTTTT 12115 0.0 17.84145 14-15 TCGCGCT 200 2.3646862E-11 17.7958 60-61 ACCGTCC 160 0.0011995544 17.794504 8 CGCGGAA 190 1.9281288E-10 17.593756 82-83 CATGGGA 9425 0.0 17.521168 4 TCGCGGA 210 4.3655746E-11 17.05517 82-83 ATGGGAT 3765 0.0 16.636965 5 >>END_MODULE