##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938943_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 432781 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03492759617451 33.0 31.0 34.0 30.0 34.0 2 32.2620725031829 34.0 31.0 34.0 30.0 34.0 3 32.33458954991092 34.0 31.0 34.0 30.0 34.0 4 35.88064171024144 37.0 35.0 37.0 35.0 37.0 5 35.805305685785655 37.0 35.0 37.0 35.0 37.0 6 35.891825195653226 37.0 35.0 37.0 35.0 37.0 7 35.84801319836129 37.0 35.0 37.0 35.0 37.0 8 35.84851229605736 37.0 35.0 37.0 35.0 37.0 9 37.45452087776496 39.0 37.0 39.0 34.0 39.0 10-11 37.36039590462613 39.0 37.0 39.0 34.0 39.0 12-13 37.3023261187529 39.0 37.0 39.0 33.5 39.0 14-15 38.37156552621303 40.0 38.0 41.0 33.5 41.0 16-17 38.47783174399985 40.0 38.0 41.0 34.0 41.0 18-19 38.60997248030759 40.0 38.0 41.0 34.0 41.0 20-21 38.59284025869897 40.0 38.0 41.0 34.0 41.0 22-23 38.52093553090362 40.0 38.0 41.0 34.0 41.0 24-25 38.40753984116678 40.0 38.0 41.0 34.0 41.0 26-27 38.10351078258981 40.0 37.0 41.0 33.5 41.0 28-29 37.92623058775686 40.0 37.0 41.0 33.0 41.0 30-31 37.8251598383478 40.0 37.0 41.0 33.0 41.0 32-33 37.69918041688521 40.0 36.0 41.0 33.0 41.0 34-35 37.575349888280684 40.0 36.0 41.0 33.0 41.0 36-37 37.383600481536845 40.0 36.0 41.0 32.5 41.0 38-39 37.16094860911177 39.5 35.5 41.0 31.5 41.0 40-41 37.035874957542035 39.0 35.0 41.0 31.0 41.0 42-43 36.91843565221209 39.0 35.0 41.0 31.0 41.0 44-45 36.74980301815468 39.0 35.0 41.0 31.0 41.0 46-47 36.45861417206393 39.0 35.0 41.0 30.0 41.0 48-49 36.13209105760188 39.0 35.0 41.0 29.5 41.0 50-51 35.908704402457595 39.0 35.0 40.0 28.5 41.0 52-53 35.62679507649365 39.0 35.0 40.0 28.0 41.0 54-55 35.42896291657905 38.0 35.0 40.0 27.5 41.0 56-57 35.37828601532877 38.0 35.0 40.0 27.5 41.0 58-59 35.182865005626404 38.0 35.0 40.0 27.0 41.0 60-61 34.7693059538196 38.0 34.0 40.0 26.0 41.0 62-63 34.31738685385911 37.5 34.0 40.0 26.0 41.0 64-65 33.80287489515482 37.0 33.0 39.5 24.0 41.0 66-67 33.275329785734584 36.0 32.5 39.0 22.5 40.0 68-69 32.78588824370756 36.0 32.0 39.0 22.0 40.0 70-71 32.13848112555773 35.0 31.5 37.5 20.5 39.5 72-73 31.54480672672784 35.0 31.0 37.0 19.0 39.0 74-75 31.02078071819234 34.5 30.5 36.5 17.5 39.0 76-77 29.008273237503495 32.5 28.0 34.5 11.5 36.5 78-79 29.958714684794387 34.0 29.0 35.0 10.5 37.0 80-81 29.94485201522248 34.0 30.0 35.0 7.0 37.0 82-83 29.529978210688547 34.0 29.0 35.0 4.0 36.5 84-85 29.068449169441358 34.0 29.0 35.0 2.0 36.0 86-87 28.438176583537633 33.5 28.5 35.0 2.0 35.5 88-89 27.6394042252317 33.0 26.5 35.0 2.0 35.0 90-91 26.96189065601309 32.5 25.0 34.5 2.0 35.0 92-93 26.638293963921704 32.0 25.0 34.0 2.0 35.0 94-95 26.116565884361837 32.0 24.5 34.0 2.0 35.0 96-97 25.14178649247541 32.0 21.0 34.0 2.0 35.0 98-99 23.953955926900676 31.0 4.5 34.0 2.0 35.0 100-101 18.503054662750905 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12561112453907697 1101 2 0.23884762685327843 1101 3 0.30972864363959474 1101 4 -0.007333847604371613 1101 5 -0.057297326366196444 1101 6 -0.0967164277258874 1101 7 -0.032144119305648644 1101 8 -0.009365562782662096 1101 9 0.23092269749073324 1101 10-11 0.28388166042203977 1101 12-13 -0.0820013443661054 1101 14-15 0.22978153946387891 1101 16-17 0.19915221290274587 1101 18-19 0.2336609962273144 1101 20-21 0.5062702171374269 1101 22-23 0.4648331216110293 1101 24-25 0.26121295401564737 1101 26-27 0.24968891514153313 1101 28-29 0.31272661806075774 1101 30-31 0.3342180168925353 1101 32-33 0.21286305698934171 1101 34-35 0.21077508116292876 1101 36-37 0.4842210709787267 1101 38-39 0.3257407261969263 1101 40-41 0.45604385041970374 1101 42-43 0.4488225402664625 1101 44-45 0.6382126287527754 1101 46-47 0.1953258633317958 1101 48-49 0.20401581012519188 1101 50-51 0.2754476111058466 1101 52-53 0.3304285775582443 1101 54-55 0.20766095259617146 1101 56-57 0.22165504445639073 1101 58-59 0.6388095237766223 1101 60-61 0.6188565623635682 1101 62-63 -0.01853868197861175 1101 64-65 0.24062255800266286 1101 66-67 0.37044194474680125 1101 68-69 0.5585993758586767 1101 70-71 0.6923280189637495 1101 72-73 0.6532850107437191 1101 74-75 0.3027189725144197 1101 76-77 -0.1595204515122468 1101 78-79 0.26292300355958886 1101 80-81 0.21530037880595287 1101 82-83 0.21324306539751703 1101 84-85 0.4532277305938628 1101 86-87 0.9776497324790867 1101 88-89 0.5210533061950393 1101 90-91 0.4667007121336333 1101 92-93 0.676306528210322 1101 94-95 0.9236100308052322 1101 96-97 0.789399522650271 1101 98-99 0.7443223773500875 1101 100-101 0.8919431282992143 1102 1 0.25319458275416906 1102 2 0.3168631228198677 1102 3 0.3946121050431586 1102 4 0.2904820501094889 1102 5 0.47297082400206847 1102 6 0.5062424728894825 1102 7 0.5002347985274653 1102 8 0.33651333910810166 1102 9 0.3836249262316116 1102 10-11 0.5109868480635313 1102 12-13 0.43189401003239425 1102 14-15 0.658672270580162 1102 16-17 0.6502729602802262 1102 18-19 0.5982721622509786 1102 20-21 0.5736425986060425 1102 22-23 0.7205006279082582 1102 24-25 0.6847215469436208 1102 26-27 0.7754645105884066 1102 28-29 0.6389262800828774 1102 30-31 0.5838784447852774 1102 32-33 0.7017703681521823 1102 34-35 0.6840238137459025 1102 36-37 0.8600333338362347 1102 38-39 0.6896121670667412 1102 40-41 0.5843734107296186 1102 42-43 0.6042955498637497 1102 44-45 0.7318006145959615 1102 46-47 0.7712082725401004 1102 48-49 0.7524614307609809 1102 50-51 0.7956529486054507 1102 52-53 0.8819893338634373 1102 54-55 0.7167672243176284 1102 56-57 0.8519359487066183 1102 58-59 0.7451322607596964 1102 60-61 0.9806427435324636 1102 62-63 1.0590973859995785 1102 64-65 1.0776757735751517 1102 66-67 0.8920725285557438 1102 68-69 1.0289083386492237 1102 70-71 1.1841177074501843 1102 72-73 0.6860274142127771 1102 74-75 0.7541617554114666 1102 76-77 1.0650435251481483 1102 78-79 1.5361940554360025 1102 80-81 1.4851246940819145 1102 82-83 1.5886822772076137 1102 84-85 1.639364260900276 1102 86-87 1.9438104951612267 1102 88-89 1.5944168237156866 1102 90-91 1.6105517467923072 1102 92-93 1.6568377273940733 1102 94-95 1.8108178871965173 1102 96-97 2.2663660118254114 1102 98-99 2.5426292995195254 1102 100-101 2.20968058963901 1103 1 0.022356151663849744 1103 2 0.005535821118328954 1103 3 -0.10621039020178813 1103 4 -0.07965366160592424 1103 5 0.007052970714660489 1103 6 0.06511514034437482 1103 7 0.08129556036368513 1103 8 0.04045360539093679 1103 9 0.2081314290846379 1103 10-11 0.00839406031870027 1103 12-13 0.07801221802088065 1103 14-15 0.020203910944118775 1103 16-17 -0.0135835142995262 1103 18-19 0.16637541110719667 1103 20-21 0.16922875498295298 1103 22-23 0.16381271344776138 1103 24-25 0.23583191552430804 1103 26-27 -0.03564110294164635 1103 28-29 -0.03995679707745836 1103 30-31 0.09589458625445246 1103 32-33 0.38341330240396587 1103 34-35 0.10728115194566357 1103 36-37 0.3214756563571868 1103 38-39 0.0032013306918869944 1103 40-41 0.29170646989787485 1103 42-43 0.43904147594199117 1103 44-45 0.16568292066700252 1103 46-47 0.28859633607785895 1103 48-49 0.4875633430624262 1103 50-51 0.2402887374297933 1103 52-53 0.6557999286303158 1103 54-55 0.6734191546111603 1103 56-57 0.3362410687395183 1103 58-59 0.3318830189974946 1103 60-61 0.2854543407154182 1103 62-63 0.04145550556982158 1103 64-65 0.5053797266929294 1103 66-67 0.2712556879655992 1103 68-69 0.5258978775378296 1103 70-71 0.35977506107173696 1103 72-73 0.039131691188050155 1103 74-75 0.05059096941444352 1103 76-77 -0.6409690436073134 1103 78-79 0.08375741327284203 1103 80-81 0.21165145088034976 1103 82-83 -0.3462570637680713 1103 84-85 0.10541062906970922 1103 86-87 0.21336724733312806 1103 88-89 -0.01067622105889754 1103 90-91 0.2519984388192391 1103 92-93 0.6408311988380646 1103 94-95 0.682328708149587 1103 96-97 0.48638027697732156 1103 98-99 0.2771239790034059 1103 100-101 0.49216593894245975 1104 1 0.07623470554240441 1104 2 0.1254149767117667 1104 3 0.08907116222261635 1104 4 0.13369187316818198 1104 5 0.25007057087511697 1104 6 0.10536735916802087 1104 7 0.2531791786758717 1104 8 0.24096255589988402 1104 9 0.25975981999874875 1104 10-11 0.1683154613115292 1104 12-13 0.14674247425113407 1104 14-15 0.42785947931397317 1104 16-17 0.08752965824221803 1104 18-19 0.22138531433138553 1104 20-21 0.34160440057287644 1104 22-23 0.22773238451029698 1104 24-25 0.18209125464078824 1104 26-27 0.019707264906720923 1104 28-29 0.04247500856863695 1104 30-31 -0.015435203646767093 1104 32-33 0.23393729578510403 1104 34-35 0.31833891657485935 1104 36-37 0.46010512534379444 1104 38-39 0.4700694047028193 1104 40-41 0.4098055522826698 1104 42-43 0.0098463949612011 1104 44-45 0.07537867857704583 1104 46-47 0.14066222385803684 1104 48-49 -0.0652778794216573 1104 50-51 0.08166165023128258 1104 52-53 0.20098808988990413 1104 54-55 0.21304458709372653 1104 56-57 0.2286061843189202 1104 58-59 0.23035930140235195 1104 60-61 0.17112851210387703 1104 62-63 -0.17227550280404103 1104 64-65 0.033892237348290166 1104 66-67 -0.3186881519782361 1104 68-69 -0.08814276328853055 1104 70-71 0.04231091325043934 1104 72-73 -0.08997689327767588 1104 74-75 0.25686322032955644 1104 76-77 -0.022146022998576598 1104 78-79 0.08707938088052103 1104 80-81 0.46031639597386587 1104 82-83 0.3147079364826446 1104 84-85 0.46643956152721344 1104 86-87 0.5179321322551544 1104 88-89 0.39882209915370836 1104 90-91 0.23611880686818054 1104 92-93 0.6692434236788607 1104 94-95 0.7634850035915939 1104 96-97 1.176707083375554 1104 98-99 2.018442988179558 1104 100-101 1.6335908750102526 1105 1 0.16045138975908912 1105 2 0.09873310002989655 1105 3 0.15909573224718798 1105 4 0.18531232478863302 1105 5 -0.039545668741119755 1105 6 0.15967296347362492 1105 7 -0.05969083419413579 1105 8 0.1761678911052229 1105 9 0.058981769220693536 1105 10-11 0.08330902630510195 1105 12-13 0.1381312656399274 1105 14-15 0.14792499937949088 1105 16-17 0.06209675780931434 1105 18-19 -0.023165405219330637 1105 20-21 0.007068891037370406 1105 22-23 -0.06373830696039562 1105 24-25 -0.19971230216277291 1105 26-27 0.06733508753454487 1105 28-29 -0.052116661023028144 1105 30-31 -0.19271085592241377 1105 32-33 -0.0866547248069196 1105 34-35 0.3363627845987267 1105 36-37 0.09009810533677154 1105 38-39 0.26017411980753735 1105 40-41 0.39612823860535684 1105 42-43 0.3495006596154582 1105 44-45 0.3939992471976197 1105 46-47 0.38179361498942654 1105 48-49 0.0823762682324869 1105 50-51 0.26715948572911685 1105 52-53 0.19500060890242565 1105 54-55 0.15811303216217 1105 56-57 0.0455097762225094 1105 58-59 0.2819680530111057 1105 60-61 0.3596040005793668 1105 62-63 0.15531604978750124 1105 64-65 0.265923944379999 1105 66-67 0.02363664034655244 1105 68-69 -0.05173817688394422 1105 70-71 0.21615261209213799 1105 72-73 0.5145568612560858 1105 74-75 0.3629209013872341 1105 76-77 0.01880986795731232 1105 78-79 0.1810363248374678 1105 80-81 0.2623317229891917 1105 82-83 0.20816470493940997 1105 84-85 -0.10938528929763791 1105 86-87 -0.07158173225870712 1105 88-89 0.21585439118600291 1105 90-91 0.1020834728328488 1105 92-93 0.06889242332785983 1105 94-95 -0.07158285647626528 1105 96-97 -0.03394285227438232 1105 98-99 0.11062738036395103 1105 100-101 0.9080672994866781 1106 1 0.03692266623036744 1106 2 -0.022005170708375488 1106 3 -0.11486004170858877 1106 4 0.05015378963010164 1106 5 -0.03739871659416849 1106 6 -0.039595785795121685 1106 7 1.079256046239152E-4 1106 8 -0.14478682984950098 1106 9 -0.2095276492887237 1106 10-11 -0.12283932984325219 1106 12-13 -0.006820179311517904 1106 14-15 -0.4357768843223866 1106 16-17 -0.3350747643622043 1106 18-19 -0.09802500507893797 1106 20-21 -0.2996732407047631 1106 22-23 -0.13590397912606988 1106 24-25 -0.3682772957277649 1106 26-27 -0.4028971576977014 1106 28-29 -0.3985569324633005 1106 30-31 -0.35450154271310197 1106 32-33 -0.07318801134020703 1106 34-35 -0.13866354042759355 1106 36-37 -0.32982116458249067 1106 38-39 -0.33214189750848533 1106 40-41 -0.031226388749274747 1106 42-43 -0.23216834705354472 1106 44-45 -0.024129920931549975 1106 46-47 -0.25982917663336735 1106 48-49 -0.2520100661538436 1106 50-51 -0.6370115684419346 1106 52-53 -0.2977834088815925 1106 54-55 -0.26852789447875836 1106 56-57 -0.37827342256068874 1106 58-59 -0.4330313619883057 1106 60-61 -0.5254316844563149 1106 62-63 -0.36146469199323406 1106 64-65 -0.5177048142487521 1106 66-67 -0.3788847121747949 1106 68-69 -0.43458010972587147 1106 70-71 -0.3360674651279396 1106 72-73 -0.46098426428504524 1106 74-75 -0.46549550202916734 1106 76-77 -0.48160548245803625 1106 78-79 -0.17827688447574275 1106 80-81 -0.11216517650770541 1106 82-83 -0.4236213018465982 1106 84-85 -0.8259437308560784 1106 86-87 -0.5275224131993923 1106 88-89 -0.07292237259076373 1106 90-91 0.3761679469173238 1106 92-93 0.36211811655355675 1106 94-95 -0.24142900132241252 1106 96-97 -0.591106184437713 1106 98-99 0.07372554346211402 1106 100-101 -0.17230593088655155 1107 1 0.02639927834848521 1107 2 -0.027434914345668915 1107 3 0.022303279417002386 1107 4 -0.008802679703727279 1107 5 0.02876071041431061 1107 6 -0.10041239142304903 1107 7 0.09554276868097134 1107 8 -0.07881863180583082 1107 9 -0.15343961442710707 1107 10-11 0.15317650159710894 1107 12-13 -0.036037647208232215 1107 14-15 -0.10710644716139228 1107 16-17 -0.1696530175725357 1107 18-19 -0.1197961329821382 1107 20-21 0.05303699526019301 1107 22-23 0.08393689519594005 1107 24-25 -0.03318040638558983 1107 26-27 -0.058383690542726185 1107 28-29 0.2638312275663637 1107 30-31 0.20667144416743355 1107 32-33 0.31167186998193586 1107 34-35 0.41126170643160975 1107 36-37 0.3777665689486369 1107 38-39 0.1756840030060971 1107 40-41 -0.12006672994810685 1107 42-43 0.35738475665050373 1107 44-45 0.051512724286560285 1107 46-47 -0.009704588065382325 1107 48-49 0.06457527991263845 1107 50-51 -0.19138723484590514 1107 52-53 0.06995568527259621 1107 54-55 0.26761437987106973 1107 56-57 0.3795843494480309 1107 58-59 0.2073162111422775 1107 60-61 0.1822342791058702 1107 62-63 0.2778340192394353 1107 64-65 0.6091292453683153 1107 66-67 0.5815656610114246 1107 68-69 0.6106832067638521 1107 70-71 0.3279113721999565 1107 72-73 0.460435118219241 1107 74-75 0.5367528870116729 1107 76-77 0.46006099104806 1107 78-79 0.7996796312166232 1107 80-81 0.9794363950466938 1107 82-83 0.4195303832862223 1107 84-85 0.6760931473600458 1107 86-87 0.8466460305445267 1107 88-89 1.0047021000808805 1107 90-91 1.1649990164087676 1107 92-93 0.9096269246755675 1107 94-95 0.9353129207025326 1107 96-97 1.251445737123639 1107 98-99 1.5856049185490377 1107 100-101 1.2411310910410336 1108 1 0.16132361983924426 1108 2 0.18036912077482725 1108 3 0.0798878114551087 1108 4 0.15367630404369947 1108 5 0.1984552742103176 1108 6 0.18468475036659981 1108 7 0.08318988244895564 1108 8 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-1.2221650139769906 2316 76-77 -0.948254385767207 2316 78-79 -0.9812568841927849 2316 80-81 -0.7317707356526135 2316 82-83 -1.0907682079570336 2316 84-85 -1.6111470112608934 2316 86-87 -1.6650626754997297 2316 88-89 -2.098150270179506 2316 90-91 -2.2974869503460233 2316 92-93 -2.2084039846787213 2316 94-95 -2.102386713527725 2316 96-97 -2.065861781074311 2316 98-99 -2.214498293706061 2316 100-101 -1.3673051527571758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 11.0 5 42.0 6 77.0 7 125.0 8 214.0 9 297.0 10 474.0 11 740.0 12 1027.0 13 1399.0 14 1702.0 15 2200.0 16 2869.0 17 3933.0 18 3841.0 19 2995.0 20 2831.0 21 3235.0 22 3518.0 23 3959.0 24 4553.0 25 5299.0 26 5975.0 27 7122.0 28 8606.0 29 10265.0 30 12483.0 31 15166.0 32 18309.0 33 22847.0 34 32116.0 35 45538.0 36 63136.0 37 88150.0 38 55064.0 39 2663.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.767525681138714 21.900346280786636 16.51455515791378 17.81757288016087 2 13.571067121708207 26.080165256792696 38.080461018390366 22.26830660310873 3 22.056883273526335 29.082376536862753 32.881988811893315 15.978751377717598 4 11.14027649088107 19.241833629480038 41.70862399227323 27.909265887365663 5 9.716923802107763 42.269877836596336 36.35996034946081 11.653238011835084 6 23.553945297968255 40.70765583516837 18.18610336405711 17.55229550280627 7 21.84037654148403 37.494483353012264 23.5740478440597 17.09109226144401 8 21.042513419027177 32.2971664652561 22.595723934276226 24.064596181440496 9 22.076754755869597 22.64678902262345 25.370337422391465 29.90611879911549 10-11 21.97081202733022 26.700802484397418 28.98556082637639 22.34282466189597 12-13 28.471443986681482 25.713004960938672 27.52951261723597 18.286038435143873 14-15 23.75227455216595 28.508298143965433 30.217259446251248 17.522167857617365 16-17 17.09420372738837 31.43705829546676 32.08105007400013 19.387687903144744 18-19 16.460710894012877 34.06992945591168 31.795674004515007 17.673685645560436 20-21 19.27025447050587 30.584406431890493 28.884239372800565 21.261099724803074 22-23 18.168658051069713 29.51354149096194 29.565992961798234 22.751807496170116 24-25 21.143834872603 30.23214974779392 29.743912047894895 18.880103331708185 26-27 21.377671385758617 27.185574228073783 33.57367814206261 17.863076244104985 28-29 17.833037956841913 30.196681462448677 33.272948673809616 18.697331906899795 30-31 18.174319112900058 30.97340225194729 31.320113406087607 19.532165229065047 32-33 17.99408938932162 31.73313985595486 31.148433041191737 19.124337713531787 34-35 20.15037472355039 28.70049616494769 30.910706486195426 20.238422625306494 36-37 20.545728952155113 28.059902604531406 32.822319140035454 18.57204930327803 38-39 21.00991030234684 28.251236621322924 32.96697836880375 17.771874707526493 40-41 18.525052887953795 29.55728941004657 33.91449424558265 18.003163456416978 42-43 17.41685326012862 31.20974953727132 30.69861054305052 20.67478665954954 44-45 18.4262242807744 30.436058501598406 30.106556647861648 21.031160569765547 46-47 19.82293400866267 30.595584556924884 29.051947136686024 20.529534297726425 48-49 19.919333222419404 29.39427129547471 30.310235507246375 20.376159974859508 50-51 19.054551000107445 30.37477601128522 31.887096979170636 18.6835760094367 52-53 18.623507039357087 31.31214170677548 31.00644436793667 19.05790688593076 54-55 16.996702720313507 29.230372867570438 32.333212409971786 21.43971200214427 56-57 19.751098130463212 30.631196840896436 29.089423981182165 20.528281047458183 58-59 20.317253091946785 29.044139309460405 29.997978175852484 20.64062942274032 60-61 18.92600080873433 29.601640575356708 31.60637744786552 19.86598116804344 62-63 20.47560400761595 29.24665415827125 31.073811856480027 19.20392997763277 64-65 19.370648033578377 30.709242718850405 30.97069286020447 18.949416387366746 66-67 18.433506096786047 29.402175294441147 30.36215625426036 21.802162354512454 68-69 17.292999407307104 30.912111615740905 31.027299688865114 20.767589288086874 70-71 20.031401599656178 30.339860506654066 28.578343987309964 21.050393906379796 72-73 18.567358548549958 28.130856021867874 30.53969559661815 22.762089832964016 74-75 17.688854177979163 32.021391881806274 29.239846481245714 21.049907458968857 76-77 19.729866297577214 27.993099229791717 30.109119585213755 22.16791488741731 78-79 18.313255239232983 28.052526928369325 32.05293361231236 21.581284220085333 80-81 19.12091593881418 30.396044179490733 29.75368547529923 20.72935440639586 82-83 18.30359928000536 29.011902093668624 30.932619500393965 21.751879125932053 84-85 17.845189420503 28.50694520663477 30.462555498688133 23.18530987417409 86-87 18.65975149318023 29.58850092221586 30.350557665955492 21.40118991864842 88-89 19.62882602742905 27.801383055252593 29.965539646810385 22.604251270507973 90-91 18.525652696777367 26.626943768286697 31.590489234112678 23.256914300823254 92-93 18.649501711027057 29.775105653898855 30.589951961846758 20.985440673227338 94-95 18.043819334206763 28.785610141019507 30.34137673674895 22.829193788024778 96-97 16.37086655837479 28.360764451304473 30.618603866620763 24.649765123699975 98-99 18.12163657831559 28.788347917306904 28.978513382056974 24.111502122320527 100-101 18.590427160439628 27.334432437990507 30.46468558251199 23.610454819057875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5371.0 1 3344.5 2 1165.0 3 925.0 4 692.5 5 667.5 6 1081.5 7 1977.0 8 2659.5 9 2650.0 10 2016.5 11 1295.5 12 1093.5 13 1013.0 14 921.0 15 793.0 16 699.0 17 705.5 18 715.0 19 741.5 20 797.0 21 876.5 22 946.5 23 1052.5 24 1156.5 25 1398.0 26 1904.0 27 2445.5 28 2782.5 29 3329.0 30 4615.0 31 5895.5 32 7963.0 33 10791.5 34 12847.5 35 14340.5 36 16496.0 37 17907.5 38 17187.0 39 16110.5 40 14874.5 41 13196.0 42 11189.0 43 11156.5 44 12483.0 45 12767.0 46 13327.5 47 21988.5 48 52594.0 49 52537.0 50 22713.5 51 10862.5 52 4318.0 53 1909.0 54 1347.5 55 1125.0 56 1009.0 57 856.5 58 767.5 59 665.5 60 521.0 61 415.0 62 297.0 63 263.5 64 235.5 65 163.5 66 113.5 67 80.0 68 56.5 69 51.5 70 39.5 71 29.5 72 30.5 73 29.5 74 25.5 75 17.0 76 11.5 77 11.0 78 7.5 79 3.0 80 3.5 81 3.0 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37617178203294505 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.087231186211964E-4 16-17 0.002657233104041074 18-19 4.621274963549694E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01432595238700405 36-37 0.025879139795878286 38-39 0.011437655534785492 40-41 0.006123189326703344 42-43 0.005083402459904663 44-45 0.0015019143631536504 46-47 0.001039786866798681 48-49 0.003003828726307301 50-51 5.776593704437117E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.087231186211964E-4 60-61 0.0013863824890649082 62-63 0.0011553187408874235 64-65 5.776593704437117E-4 66-67 0.0027727649781298163 68-69 0.002426169355863589 70-71 1.1553187408874235E-4 72-73 0.0 74-75 0.0 76-77 0.08445379995887066 78-79 0.0028882968522185586 80-81 2.310637481774847E-4 82-83 0.0 84-85 1.1553187408874235E-4 86-87 0.09207890364872764 88-89 0.42769899787652416 90-91 0.3760562501588563 92-93 0.0 94-95 9.242549927099388E-4 96-97 0.0 98-99 0.0 100-101 0.001039786866798681 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 432781.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.87060199038313 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39050529205969 15.108334238333013 2 10.73800983127408 4.482174587146847 3 4.2779327753421015 2.6784909688734024 4 2.424604756210974 2.024118434034766 5 1.4868694920508392 1.5515930690118096 6 1.1724458615650326 1.4681790559197379 7 0.8270227182144281 1.2082323392200673 8 0.6122403790797574 1.0222260219371921 9 0.5325273459988485 1.0002749658603312 >10 4.5569283911252825 19.638570085100778 >50 0.6144546299986714 8.925992592096234 >100 0.2978167485939507 10.90412934024368 >500 0.023249634648598378 3.2820294791129925 >1k 0.04096364199991143 19.542447565858943 >5k 0.004428501837828263 7.163207257250202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9565 2.210124751317641 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8616 1.9908452542972082 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7324 1.6923108916518979 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5496 1.2699263599834558 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4932 1.1396064060113544 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4743 1.09593535760581 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 1.0171426194772875 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3629 0.8385303421360919 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3308 0.7643588789711194 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3172 0.7329342092189814 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2896 0.6691606147219957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.6548346623349915 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2806 0.648364877386022 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2747 0.6347321162435504 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2727 0.6301108412800007 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2663 0.6153227613966418 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2661 0.6148606339002868 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2570 0.5938338328161357 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2558 0.5910610678380058 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2483 0.5737312867246945 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2233 0.5159653496803234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2211 0.5108819472204186 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2175 0.5025636522860292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2091 0.48315429743912053 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2041 0.47160111003024624 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2018 0.4662866438221641 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1876 0.43347559158096133 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1713 0.3958122006280313 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1592 0.3678534870985556 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1573 0.36346327588318345 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1545 0.35699349093421384 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1454 0.3359666898500627 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1436 0.331807542382868 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1371 0.31678839875133147 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1312 0.3031556376088599 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1256 0.2902160677109208 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1253 0.2895228764663883 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 1188 0.2745037328348518 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1090 0.2518594855134583 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1010 0.23337438565925953 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1007 0.23268119441472707 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 953 0.22020375201314293 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 942 0.21766205078319056 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 931 0.21512034955323825 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 876 0.20241184340347657 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 802 0.1853131260383427 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 792 0.18300248855656787 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 735 0.16983185491045125 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 692 0.1598961137388194 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 684 0.15804760375339952 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 680 0.1571233487606896 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 660 0.1525020737971399 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 613 0.14164207763279812 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 595 0.1374829301656034 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 561 0.1296267627275689 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 541 0.1250054877640192 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 540 0.12477442401584173 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 530 0.12246378653406689 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 528 0.12200165903771192 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 522 0.12061527654864701 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 521 0.12038421280046953 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 506 0.11691825657780726 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 499 0.11530081034056484 No Hit TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC 462 0.10675145165799792 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 452 0.10444081417622308 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 444 0.10259230419080319 No Hit GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA 438 0.10120592170173828 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.931912445324541E-4 0.0 10-11 0.0 0.0 0.0 6.931912445324541E-4 0.0 12-13 0.0 0.0 0.0 9.242549927099388E-4 0.0 14-15 0.0 0.0 0.0 9.242549927099388E-4 0.0 16-17 0.0 0.0 0.0 0.001039786866798681 0.0 18-19 0.0 0.0 0.0 0.0011553187408874235 0.0 20-21 0.0 0.0 0.0 0.001270850614976166 0.0 22-23 0.0 0.0 0.0 0.003003828726307301 0.0 24-25 0.0 0.0 0.0 0.00739403994167951 0.0 26-27 0.0 0.0 0.0 0.012939569897939141 0.0 28-29 0.0 0.0 0.0 0.03523722159706641 0.0 30-31 0.0 0.0 0.0 0.08387614058842693 0.0 32-33 0.0 0.0 0.0 0.1680988767991201 0.0 34-35 0.0 0.0 0.0 0.301769255119795 0.0 36-37 0.0 0.0 0.0 0.48858429552129135 0.0 38-39 0.0 0.0 0.0 0.7188393205801549 0.0 40-41 0.0 0.0 0.0 0.943895411305025 0.0 42-43 0.0 0.0 0.0 1.1681427789112737 0.0 44-45 0.0 0.0 0.0 1.4256633262550804 0.0 46-47 0.0 0.0 0.0 1.7624387392237644 0.0 48-49 0.0 0.0 0.0 2.10822563837137 0.0 50-51 0.0 0.0 0.0 2.4313682901975824 0.0 52-53 0.0 0.0 0.0 2.716732019196776 0.0 54-55 0.0 0.0 0.0 3.031671907962688 0.0 56-57 0.0 0.0 0.0 3.4247113436125893 0.0 58-59 0.0 0.0 0.0 3.8565694889563082 0.0 60-61 0.0 0.0 0.0 4.401764402781083 0.0 62-63 0.0 0.0 0.0 4.889193379561487 0.0 64-65 0.0 0.0 0.0 5.340691943500293 0.0 66-67 0.0 0.0 0.0 5.875604520531169 0.0 68-69 0.0 0.0 0.0 6.448873679759509 0.0 70-71 0.0 0.0 0.0 7.088458134714786 0.0 72-73 0.0 0.0 0.0 7.73393471524859 0.0 74-75 0.0 0.0 0.0 8.462594245126287 0.0 76-77 0.0 0.0 0.0 9.1192774174467 0.0 78-79 0.0 0.0 0.0 9.950413719641112 0.0 80-81 0.0 0.0 0.0 10.822910432759294 0.0 82-83 0.0 0.0 0.0 11.598244839768842 0.0 84-85 0.0 0.0 0.0 12.596786827517844 0.0 86-87 0.0 0.0 0.0 13.644545393628647 0.0 88-89 0.0 0.0 0.0 14.574576980043023 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 35 2.7504045E-4 54.42718 1 CATATAG 35 2.799591E-4 54.232235 3 ACGCTCC 25 0.0015608303 38.267647 86-87 GGATATG 75 6.7898764E-6 38.099026 1 CGGTATA 35 2.0606334E-4 33.906902 34-35 GTAAGCG 80 5.4569682E-11 32.62785 94-95 ATCTAGA 75 3.028695E-4 31.74919 1 CTCCCGC 90 2.425799E-5 31.635471 7 ATATGAG 105 1.9127074E-6 31.635471 3 GGACGTG 60 0.003967349 31.635471 6 GATTGGC 90 2.425799E-5 31.635471 9 CCCTGTA 75 3.0931292E-4 31.635471 2 GACAATG 240 0.0 29.658255 7 AGGAGCC 85 6.437669E-4 27.91365 7 GTATAGG 205 3.092282E-11 27.877337 1 GCTTGTC 60 5.416181E-6 27.684235 94-95 AGCCACG 35 0.0081912745 27.27685 82-83 GAGGTGC 105 6.942478E-5 27.11612 6 GGATGAC 70 0.008426303 27.116117 8 AGATTGG 140 5.842121E-7 27.116117 8 >>END_MODULE