##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938942_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436504 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.681689056686764 33.0 31.0 34.0 30.0 34.0 2 31.718559280098233 33.0 31.0 34.0 30.0 34.0 3 31.7330058831076 33.0 31.0 34.0 30.0 34.0 4 35.249266902479704 37.0 35.0 37.0 33.0 37.0 5 35.30692960431061 37.0 35.0 37.0 33.0 37.0 6 35.4421311144915 37.0 35.0 37.0 33.0 37.0 7 35.390754723897146 37.0 35.0 37.0 33.0 37.0 8 35.39187957040485 37.0 35.0 37.0 33.0 37.0 9 36.92589071348716 39.0 37.0 39.0 33.0 39.0 10-11 36.82183324780529 39.0 37.0 39.0 32.5 39.0 12-13 36.75360019610359 39.0 37.0 39.0 32.5 39.0 14-15 37.75115691952422 40.0 37.0 41.0 32.0 41.0 16-17 37.693653437311 40.0 37.0 41.0 32.5 41.0 18-19 37.73333348606198 40.0 37.0 41.0 32.5 41.0 20-21 37.601712699081794 40.0 37.0 41.0 32.0 41.0 22-23 37.72020645858915 40.0 37.0 41.0 32.0 41.0 24-25 37.6123288675476 40.0 37.0 41.0 32.0 41.0 26-27 37.419691457581145 40.0 36.5 41.0 31.5 41.0 28-29 37.287465865146714 40.0 36.5 41.0 31.5 41.0 30-31 37.196220424096914 40.0 36.0 41.0 31.0 41.0 32-33 36.99824858420541 39.0 35.5 41.0 31.0 41.0 34-35 36.68574171141616 39.0 35.0 41.0 30.5 41.0 36-37 36.3521559939886 39.0 35.0 41.0 30.0 41.0 38-39 36.062561855103276 39.0 35.0 40.5 30.0 41.0 40-41 35.96005878525741 39.0 35.0 40.0 30.0 41.0 42-43 35.825361279621724 39.0 35.0 40.0 29.5 41.0 44-45 35.79988384986163 39.0 35.0 41.0 29.0 41.0 46-47 35.537560938731374 39.0 35.0 41.0 27.0 41.0 48-49 35.34821674028188 39.0 35.0 41.0 26.0 41.0 50-51 34.14747860271612 38.0 34.0 40.0 23.5 40.5 52-53 34.31961333687664 38.0 33.5 39.5 23.5 40.5 54-55 34.91271901288419 38.5 35.0 40.0 24.5 41.0 56-57 34.86314100214431 39.0 35.0 40.0 23.5 41.0 58-59 34.75258302329417 39.0 35.0 40.0 24.0 41.0 60-61 34.40856899364038 38.0 34.0 40.0 22.5 41.0 62-63 34.03614399868043 38.0 34.0 40.0 22.0 41.0 64-65 33.51460925902168 37.0 33.0 40.0 21.5 41.0 66-67 33.046830040503636 37.0 33.0 39.0 19.0 41.0 68-69 32.64139847515716 36.0 32.0 39.0 18.0 40.5 70-71 32.046826604109015 35.5 31.5 38.5 13.5 40.0 72-73 31.489958854901673 35.0 31.0 37.0 9.0 39.5 74-75 30.98809518354929 35.0 31.0 37.0 6.5 39.0 76-77 30.49567014277074 35.0 30.5 36.5 2.0 39.0 78-79 29.994236020746662 34.0 29.5 36.0 2.0 38.0 80-81 29.530690440408335 34.0 29.0 35.5 2.0 37.0 82-83 29.121904953906494 34.0 29.0 35.0 2.0 37.0 84-85 28.72061653501457 34.0 29.0 35.0 2.0 36.0 86-87 28.31714256914026 34.0 29.0 35.0 2.0 36.0 88-89 27.740756098454995 34.0 27.0 35.0 2.0 35.5 90-91 27.161100471015157 33.5 25.5 35.0 2.0 35.0 92-93 26.700263915107307 33.0 25.0 35.0 2.0 35.0 94-95 26.189955876693 33.0 24.5 35.0 2.0 35.0 96-97 25.446026840532962 33.0 19.5 35.0 2.0 35.0 98-99 24.69949759910562 32.0 9.5 35.0 2.0 35.0 100-101 22.15928834558217 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.19194149087983803 1101 2 0.0384502823583297 1101 3 0.1044377072009155 1101 4 -0.13440638472388855 1101 5 -0.3924727757502211 1101 6 -0.14311045145787205 1101 7 -0.22080821958957841 1101 8 0.02211888270414164 1101 9 -0.1329288705615923 1101 10-11 -0.03161350372042904 1101 12-13 0.004150435785334139 1101 14-15 0.09538871652727465 1101 16-17 0.2290710571751049 1101 18-19 0.1849338637895741 1101 20-21 0.16050866104326644 1101 22-23 0.13005001170171937 1101 24-25 0.36280708651619165 1101 26-27 0.23874637338888505 1101 28-29 0.07803148674116756 1101 30-31 0.5312881638475915 1101 32-33 0.23523509684555677 1101 34-35 0.27699774888792916 1101 36-37 0.2047171176785767 1101 38-39 0.22177332469854605 1101 40-41 0.26750812246127254 1101 42-43 0.30178094537578914 1101 44-45 0.4230420306222413 1101 46-47 0.6858726481021762 1101 48-49 0.9526269448910725 1101 50-51 0.8459473127712442 1101 52-53 0.9507419763012805 1101 54-55 1.2343881151376905 1101 56-57 0.9587526388565166 1101 58-59 0.5893450177942157 1101 60-61 0.8273829124843814 1101 62-63 0.9888202160620168 1101 64-65 1.1849857216213096 1101 66-67 1.0696554567417493 1101 68-69 1.140533850816631 1101 70-71 0.9429217466701516 1101 72-73 1.0112521033269282 1101 74-75 0.8780918090094261 1101 76-77 0.655070298249246 1101 78-79 0.750456143225037 1101 80-81 0.7339581188495856 1101 82-83 0.6468707605725683 1101 84-85 0.36343986940358164 1101 86-87 0.19342402355416155 1101 88-89 0.4592497667163755 1101 90-91 0.5890009561767151 1101 92-93 0.41236732921167985 1101 94-95 0.21784470436657344 1101 96-97 0.5010615574675334 1101 98-99 0.5811526396399778 1101 100-101 1.0127563711605418 1102 1 0.18363220406281044 1102 2 0.3926670585557481 1102 3 0.19453701087572028 1102 4 0.32229582419892466 1102 5 0.11852133420982369 1102 6 0.23388367112818997 1102 7 0.3721161568426865 1102 8 0.24842141485315494 1102 9 0.3548144171286651 1102 10-11 0.48644486638618645 1102 12-13 0.5446705016931759 1102 14-15 0.4236495449206714 1102 16-17 0.8057204893196968 1102 18-19 0.8044612717300765 1102 20-21 0.704914385682315 1102 22-23 0.8184997254066317 1102 24-25 0.6880155737291886 1102 26-27 0.5849582506891835 1102 28-29 0.649460058169737 1102 30-31 0.9559076470644499 1102 32-33 0.7933937175787449 1102 34-35 0.6865196645082179 1102 36-37 0.8022689317225016 1102 38-39 0.6882386062904331 1102 40-41 0.621586012400229 1102 42-43 0.7045416254134054 1102 44-45 0.5416982429414574 1102 46-47 0.9253094011939709 1102 48-49 1.0792249259899123 1102 50-51 0.7833348622494753 1102 52-53 0.7435047396327832 1102 54-55 0.659951393610946 1102 56-57 1.0388900099921656 1102 58-59 0.7929017686133193 1102 60-61 0.943703310456641 1102 62-63 0.7778141113796551 1102 64-65 0.564422979754049 1102 66-67 0.6652439241318859 1102 68-69 0.6941218510438958 1102 70-71 0.9271486868164871 1102 72-73 0.7649625759821213 1102 74-75 0.7318256398080827 1102 76-77 0.5922337358104066 1102 78-79 0.8744089724012554 1102 80-81 1.0417900412247931 1102 82-83 0.8830405300845072 1102 84-85 0.985464073490629 1102 86-87 0.905768486727208 1102 88-89 1.1951291376878856 1102 90-91 0.9523684429036088 1102 92-93 1.345537786841902 1102 94-95 0.9257227117275484 1102 96-97 0.9059812875231508 1102 98-99 0.9814922796720502 1102 100-101 1.1895932997540655 1103 1 0.46155811178536155 1103 2 0.528859231560606 1103 3 0.5733844643376571 1103 4 0.5419124611713357 1103 5 0.5239768849869932 1103 6 0.24306841033074278 1103 7 0.5983372049473772 1103 8 0.5522857633203202 1103 9 0.6622236448733361 1103 10-11 0.6533122252360997 1103 12-13 0.8743319872249131 1103 14-15 0.9112240365346054 1103 16-17 0.8568417033026208 1103 18-19 0.7855726558917766 1103 20-21 0.9069479299472363 1103 22-23 0.7813184266415121 1103 24-25 0.7381906674196657 1103 26-27 0.7632497048883664 1103 28-29 0.9799478630477836 1103 30-31 0.6784670867646412 1103 32-33 0.7822122959408588 1103 34-35 0.7198243275296576 1103 36-37 0.6406820299592724 1103 38-39 0.9845124896841853 1103 40-41 0.984266481774192 1103 42-43 0.7025664457119873 1103 44-45 0.9868570559905407 1103 46-47 0.8698722813315953 1103 48-49 1.0400008675024779 1103 50-51 0.798908985245113 1103 52-53 1.0824554062175409 1103 54-55 1.211296850390454 1103 56-57 1.146606419649963 1103 58-59 0.8662568094685241 1103 60-61 0.5829058662101545 1103 62-63 0.7906051662423508 1103 64-65 0.5581533969070804 1103 66-67 0.674612972482322 1103 68-69 0.9313645861916413 1103 70-71 0.9299716885981439 1103 72-73 0.8680159641986229 1103 74-75 0.7070483466950996 1103 76-77 0.6647133082131838 1103 78-79 0.7885024785756087 1103 80-81 1.1610288444440613 1103 82-83 1.1732690734278748 1103 84-85 0.6794402361741696 1103 86-87 1.0869505227289302 1103 88-89 1.3030482874083482 1103 90-91 1.2447011823519105 1103 92-93 1.4280314896126818 1103 94-95 1.2345480480917423 1103 96-97 0.8403487999640191 1103 98-99 0.7381243371765045 1103 100-101 0.6801382404679579 1104 1 -0.27054765976740214 1104 2 -0.134965931443503 1104 3 -0.28027407224770684 1104 4 0.0913932629100529 1104 5 0.03658253579597215 1104 6 -0.03584972538526898 1104 7 -0.11926723842378806 1104 8 0.002056350135099194 1104 9 0.21091961203102727 1104 10-11 -0.18550690057892183 1104 12-13 0.22824408575183952 1104 14-15 0.02472319132812828 1104 16-17 0.3483171923149371 1104 18-19 0.3523077440347393 1104 20-21 0.19003172613173547 1104 22-23 0.08169273236476471 1104 24-25 0.3188233648357581 1104 26-27 0.3139560560112784 1104 28-29 0.2224798601499316 1104 30-31 0.39778609183587577 1104 32-33 0.3343132377423572 1104 34-35 0.22699650816235106 1104 36-37 0.11295925768204285 1104 38-39 0.28064265148085354 1104 40-41 0.017700434925608022 1104 42-43 0.24239981929324728 1104 44-45 0.25839774664496673 1104 46-47 0.4856474564100779 1104 48-49 0.6719375977608664 1104 50-51 0.263380127481291 1104 52-53 0.35837305895842064 1104 54-55 0.4197308035418672 1104 56-57 0.26930625060867186 1104 58-59 0.3058547326754706 1104 60-61 0.5620413407332165 1104 62-63 0.6037300153126139 1104 64-65 0.4384721604907611 1104 66-67 0.48519616494790796 1104 68-69 0.5008405581790853 1104 70-71 0.5095176919789708 1104 72-73 0.35269357830149417 1104 74-75 0.7337085736934128 1104 76-77 0.7407517047357608 1104 78-79 0.7782452943205982 1104 80-81 1.0154601412322748 1104 82-83 1.049349730274045 1104 84-85 1.2233185264422204 1104 86-87 1.0492362025164184 1104 88-89 1.2745708664955266 1104 90-91 1.163525137918196 1104 92-93 1.004764162880008 1104 94-95 0.6496047976691379 1104 96-97 0.5180594978630779 1104 98-99 0.3488826081255496 1104 100-101 0.41681416415788775 1105 1 0.2753716517899214 1105 2 0.40866319782456273 1105 3 0.3972960212102379 1105 4 0.3457191627897558 1105 5 0.6681870513877186 1105 6 0.5749580843676725 1105 7 0.3453853015965649 1105 8 0.5216269987887472 1105 9 0.4729257191837988 1105 10-11 0.4784261983384468 1105 12-13 0.7810108110224974 1105 14-15 0.8725301682875113 1105 16-17 1.0632241970341028 1105 18-19 0.6912200231131109 1105 20-21 0.7162994229948723 1105 22-23 0.6082958599672494 1105 24-25 0.7610251784750659 1105 26-27 0.7948943367552417 1105 28-29 0.8702544506931034 1105 30-31 0.9218908387591682 1105 32-33 0.7339731803721321 1105 34-35 0.8618974982613636 1105 36-37 0.9154483850994595 1105 38-39 0.8316859567459574 1105 40-41 0.9234675303267323 1105 42-43 0.8280850233276738 1105 44-45 0.8160455675018241 1105 46-47 1.0023033276046718 1105 48-49 1.0857781640604927 1105 50-51 0.9520888348006196 1105 52-53 1.17962317462419 1105 54-55 0.8523670578214961 1105 56-57 0.8435405431968093 1105 58-59 0.8176757745927503 1105 60-61 1.132643728074754 1105 62-63 1.0312422758844733 1105 64-65 1.1786058701234055 1105 66-67 0.9586624368087371 1105 68-69 1.125979357105713 1105 70-71 1.2117245949122548 1105 72-73 1.2630410382811412 1105 74-75 1.3149637786531656 1105 76-77 1.1608382227333536 1105 78-79 1.0068008601575542 1105 80-81 0.9821252656886479 1105 82-83 1.1199752507700218 1105 84-85 1.497943769340342 1105 86-87 1.6750574294989313 1105 88-89 1.744671264385783 1105 90-91 1.6836856820099904 1105 92-93 1.849760461575297 1105 94-95 1.7566303366716376 1105 96-97 1.4983269171283702 1105 98-99 1.5389677381338807 1105 100-101 1.7973082883366587 1106 1 0.30728247264343267 1106 2 0.18786599030288897 1106 3 0.2655090602824117 1106 4 0.17285574650780688 1106 5 0.21013615621825465 1106 6 0.17174898629313162 1106 7 0.2680404080035004 1106 8 0.14805420293242832 1106 9 0.48617310123537294 1106 10-11 0.2747638855358403 1106 12-13 0.3326041000665185 1106 14-15 0.09766247320812482 1106 16-17 0.4388575716540686 1106 18-19 0.4219963996472842 1106 20-21 0.5857313563357138 1106 22-23 0.6047095521190258 1106 24-25 0.5245850253391566 1106 26-27 0.3638815003629503 1106 28-29 0.3205443955191356 1106 30-31 0.36600749228917806 1106 32-33 0.7238814114271932 1106 34-35 0.565813160911965 1106 36-37 0.17622419863397454 1106 38-39 0.25311372388929243 1106 40-41 0.7348541984947374 1106 42-43 0.7013763193528959 1106 44-45 0.9041212346564222 1106 46-47 0.8052675208261562 1106 48-49 0.9596746847225788 1106 50-51 0.6829524762824377 1106 52-53 0.6981768284920022 1106 54-55 0.8209207116893111 1106 56-57 0.7599182033702121 1106 58-59 0.8712817874291403 1106 60-61 1.1070610234243787 1106 62-63 0.6940139232214833 1106 64-65 0.44982251239341764 1106 66-67 0.5573928725704818 1106 68-69 0.3834362876315538 1106 70-71 0.6118259348514528 1106 72-73 0.5202052089826346 1106 74-75 0.6143360340298045 1106 76-77 0.41547733994988434 1106 78-79 0.18497617825236645 1106 80-81 -0.0026061834724835364 1106 82-83 0.04307512691009663 1106 84-85 0.08862330993261835 1106 86-87 0.44063844515032 1106 88-89 0.675748845739232 1106 90-91 0.7226030336595777 1106 92-93 0.5876700438295508 1106 94-95 0.8823734962257426 1106 96-97 1.0491939366081589 1106 98-99 1.0595172258041856 1106 100-101 1.4052043585990184 1107 1 0.11382292014773299 1107 2 0.1430868738370279 1107 3 0.11258307060943551 1107 4 0.1602051440981711 1107 5 0.2632916352773478 1107 6 0.05927050092307695 1107 7 -0.30915408425123303 1107 8 0.03439958445852653 1107 9 0.20717138006497748 1107 10-11 0.18380003011414914 1107 12-13 -0.1724159802547618 1107 14-15 -0.08700476718200179 1107 16-17 0.10225401456858663 1107 18-19 0.4039814828371888 1107 20-21 0.23049612971495037 1107 22-23 0.052543746037564176 1107 24-25 0.2279699937843631 1107 26-27 0.341879205469084 1107 28-29 0.4732695819792667 1107 30-31 0.23360645958693738 1107 32-33 0.10979650035432797 1107 34-35 -0.153578692214829 1107 36-37 0.0013963156735599114 1107 38-39 0.20579588108952152 1107 40-41 0.15804696957906827 1107 42-43 0.44307167219282917 1107 44-45 0.6769267424016903 1107 46-47 0.7017247784279874 1107 48-49 0.5098324586755325 1107 50-51 0.41618540800934056 1107 52-53 0.4182858359504067 1107 54-55 0.2707916239096164 1107 56-57 0.4479130398590243 1107 58-59 0.5869014087716522 1107 60-61 0.18540296260969313 1107 62-63 0.4032939002725442 1107 64-65 0.6369280775110369 1107 66-67 0.44891610837082396 1107 68-69 0.310458662846699 1107 70-71 0.18577888952729182 1107 72-73 0.12710423365274792 1107 74-75 0.14525972880892368 1107 76-77 0.3197946090261894 1107 78-79 0.4446290755558664 1107 80-81 0.0686072416566077 1107 82-83 0.440981036261789 1107 84-85 0.2809210724111004 1107 86-87 0.18114332179977666 1107 88-89 0.497908914586052 1107 90-91 0.5486500789837301 1107 92-93 0.4482695848507774 1107 94-95 0.5600126241660739 1107 96-97 0.9125903795226726 1107 98-99 0.6411150456550168 1107 100-101 0.5653879501079118 1108 1 -0.16198022407646562 1108 2 0.011109202385672035 1108 3 -0.19654201483144007 1108 4 -0.02663696116498926 1108 5 0.15127295220603543 1108 6 0.2723600429599884 1108 7 -0.028737132781650132 1108 8 0.05561170567064977 1108 9 0.04045914493168823 1108 10-11 0.06083352293712352 1108 12-13 -0.18262331677789234 1108 14-15 0.2525999263700598 1108 16-17 0.13497217360253444 1108 18-19 0.3152027177426291 1108 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2316 74-75 -2.840454556905364 2316 76-77 -2.8847292004976204 2316 78-79 -2.719418543491752 2316 80-81 -2.6521070440576793 2316 82-83 -2.7318761065953545 2316 84-85 -2.8821741656841375 2316 86-87 -2.9493328686764144 2316 88-89 -3.0170910854139485 2316 90-91 -3.3723023019686487 2316 92-93 -3.9581589865777964 2316 94-95 -3.6721302329767838 2316 96-97 -3.995981049048755 2316 98-99 -4.411027811487841 2316 100-101 -4.608897764177804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2237.0 3 452.0 4 336.0 5 375.0 6 416.0 7 478.0 8 632.0 9 807.0 10 1031.0 11 1345.0 12 1848.0 13 2776.0 14 2627.0 15 2577.0 16 3304.0 17 4278.0 18 3701.0 19 2784.0 20 2878.0 21 3130.0 22 3482.0 23 4010.0 24 4610.0 25 5310.0 26 5999.0 27 6897.0 28 8242.0 29 9339.0 30 11127.0 31 13418.0 32 16444.0 33 20881.0 34 27934.0 35 43397.0 36 55214.0 37 80720.0 38 70811.0 39 10657.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70746361244816 21.107881767740007 16.190651242403703 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18.3051733889966 30.57159675927366 29.964622953954546 21.158606897775197 46-47 19.86299791974309 30.453187308359496 29.021311690675244 20.66250308122217 48-49 19.7502416588802 29.45588443693769 30.256613503633233 20.537260400548874 50-51 19.198357685045554 30.224885172739143 31.965812753515664 18.61094438869964 52-53 18.54877916715629 31.170004170439647 31.045467008596635 19.235749653807428 54-55 17.084782859117613 28.99873286487732 32.539569174058286 21.376915101946782 56-57 19.80873719098872 30.48527409959762 29.232760665826746 20.473228043586914 58-59 20.283983601271363 28.829632871159426 30.173775853332717 20.71260767423649 60-61 19.0299808813898 29.067884303057024 32.012564644728805 19.88957017082438 62-63 20.73843020804777 28.682072322574253 31.34711564378241 19.232381825595564 64-65 19.548560301420686 30.193342474276697 31.344525227851456 18.913571996451164 66-67 18.638680949922563 28.869662216977655 30.753443284903014 21.738213548196768 68-69 17.39850421194556 30.482501123569612 31.617018334351265 20.501976330133562 70-71 20.059599741411795 29.890906660490053 28.995873420843083 21.05362017725507 72-73 18.542421393213683 27.902989408478746 31.12273351585041 22.431855682457158 74-75 17.952464131481797 31.530881722610648 29.929415180690683 20.58723896521687 76-77 19.896201146280347 27.602776417729903 30.55902767777426 21.94199475821549 78-79 18.46532863108963 27.568938470787963 32.811855735408955 21.15387716271345 80-81 19.449217742232705 29.96109442376442 30.50632532340692 20.083362510595954 82-83 18.574480951421084 28.53145967114925 31.603420969274627 21.29063840815504 84-85 18.243930535955993 27.947239581833365 31.121158886040952 22.687670996169686 86-87 19.17752203806454 29.01168797809613 31.132502531224194 20.678287452615137 88-89 20.183013794581242 27.114635343343835 30.721801981977414 21.98054888009751 90-91 18.993521269891016 26.137061493207298 32.403444108134764 22.465973128766922 92-93 19.426164810536445 29.229989226186703 31.277244208355174 20.066601754921674 94-95 18.636408155740302 28.233287818817658 31.17594529039989 21.954358735042145 96-97 17.134524957627168 27.462434798839947 31.407169794363575 23.995870449169306 98-99 18.893263689881536 28.04981050851012 29.857498251423927 23.19942755018442 100-101 19.52505258889424 26.48637066357623 31.313259313360692 22.675317434168843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6304.0 1 3727.0 2 1093.0 3 907.0 4 673.0 5 721.5 6 1209.0 7 2085.0 8 2759.0 9 2764.5 10 2080.0 11 1375.5 12 1183.5 13 1124.0 14 1041.5 15 967.0 16 1033.5 17 989.0 18 818.5 19 790.0 20 806.5 21 902.5 22 1024.0 23 1166.5 24 1308.0 25 1485.5 26 2000.0 27 2562.5 28 2729.0 29 3294.0 30 4615.5 31 5862.0 32 8000.0 33 10651.5 34 12597.0 35 14249.0 36 16591.0 37 17987.5 38 17135.0 39 16014.0 40 14747.5 41 13220.5 42 11238.0 43 10857.0 44 12284.0 45 12864.0 46 14068.0 47 22342.5 48 53484.5 49 53403.0 50 20684.5 51 8715.0 52 4130.5 53 2472.5 54 1783.0 55 1417.5 56 1168.0 57 982.5 58 924.0 59 796.5 60 609.0 61 528.0 62 431.0 63 355.0 64 317.0 65 223.5 66 184.0 67 162.5 68 113.0 69 94.5 70 74.5 71 55.0 72 54.0 73 52.0 74 43.5 75 40.0 76 31.0 77 20.5 78 15.0 79 10.5 80 6.5 81 5.5 82 7.0 83 4.5 84 2.0 85 1.5 86 1.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28842805564210183 2 0.09507358466359989 3 0.10194637391639023 4 0.11477558052159888 5 0.11683741729743599 6 0.09232446896248372 7 0.10194637391639023 8 0.09850997928999505 9 0.10400821069222732 10-11 0.0956463171013324 12-13 0.10263365284166927 14-15 0.1111100929201107 16-17 0.09931180470282058 18-19 0.11065190696992469 20-21 0.11271374374576178 22-23 0.12588658981360995 24-25 0.1383721569561791 26-27 0.1399758077818302 28-29 0.13390484394186536 30-31 0.12519931088833092 32-33 0.12119018382420323 34-35 0.3825852684053296 36-37 0.5715869728570643 38-39 0.5541759067499954 40-41 0.5204992394113227 42-43 0.5442103623334494 44-45 0.5648287300918204 46-47 0.5550922786503675 48-49 0.5783452156223082 50-51 0.5687233106684016 52-53 0.5720451588072504 54-55 0.5621941608782508 56-57 0.5353902827923684 58-59 0.5324120741161593 60-61 0.4835007239338013 62-63 0.5546340927001814 64-65 0.586248923263017 66-67 0.5993072228433187 68-69 0.5999945017685977 70-71 0.5978181185052142 72-73 0.5892271319392262 74-75 0.586019830287924 76-77 0.5707851474442388 78-79 0.546386745596833 80-81 0.5443249088209959 82-83 0.5536031743122629 84-85 0.5650578230669134 86-87 0.5556650110881001 88-89 0.5711287869068783 90-91 0.5831561680992614 92-93 0.5914035152026098 94-95 0.5902580503271448 96-97 0.5861343767754706 98-99 0.5909453292524238 100-101 0.5674632993053901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 436504.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.981321924570597 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97298791613632 17.2297837094767 2 9.73399843111081 4.474003031172454 3 3.841015344286549 2.648148304327936 4 2.286999784163805 2.102331131251675 5 1.3639997816199902 1.5673259043226493 6 1.0389998325558456 1.4326553778924502 7 0.7349998815356923 1.1823888224465098 8 0.6189999002319312 1.1380348782805674 9 0.5109999176389588 1.0569108249620978 >10 4.0409993486869515 19.207096329170955 >50 0.5399999129648488 8.53526158710422 >100 0.2579999584165389 10.509816448612012 >500 0.017999997098828293 2.9482733145115785 >1k 0.03499999435883279 17.768235995383677 >5k 0.004999999194118971 8.199734341084506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8880 2.0343456188259443 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8483 1.943395707714019 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6937 1.5892179682202225 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5941 1.3610413650275828 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5439 1.246036691530891 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4883 1.1186609973791763 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4395 1.0068636255337866 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3902 0.8939207888129318 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3250 0.7445521690522882 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3159 0.723704708318824 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2874 0.6584132104173157 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2852 0.6533731649652695 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2828 0.6478749335630373 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2743 0.6284020306801312 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2568 0.5883107600388542 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2509 0.5747942745083665 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2477 0.5674632993053901 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2399 0.5495940472481352 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2370 0.5429503509704379 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2239 0.5129391712332533 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2198 0.5035463592544398 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2156 0.49392445430053333 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2134 0.48888440884848705 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2123 0.4863643861224639 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1931 0.4423785349046057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1889 0.4327566299506992 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1659 0.3800652456793065 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1657 0.37960705972912046 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1621 0.37135971262577205 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1575 0.3608214357714935 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1510 0.34593039239044776 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1396 0.3198137932298444 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1395 0.3195847002547514 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1318 0.30194454117258945 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1317 0.3017154481974965 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1312 0.3005699833220314 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 1279 0.293009915143962 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1254 0.2872825907666367 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1137 0.26047871268075434 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1007 0.23069662591866286 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 980 0.22451111559115153 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 973 0.22290746476550044 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 918 0.21030735113538476 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 856 0.19610358667961808 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 846 0.19381265692868793 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 827 0.1894598904019207 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 777 0.17800524164727013 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 741 0.1697578945439217 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.16494694206696847 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 674 0.15440866521268992 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 635 0.14547403918406246 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.13608122720524898 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 593 0.13585213423015596 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 568 0.13012480985283068 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.1285211590271796 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 535 0.12256474167476128 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 524 0.12004471894873817 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 507 0.11615013837215696 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 490 0.11225555779557576 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 483 0.11065190696992469 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 459 0.10515367556769238 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 454 0.10400821069222732 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 452 0.10355002474204131 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 445 0.10194637391639023 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 438 0.10034272309073913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1454648754650588E-4 0.0 14-15 0.0 0.0 0.0 3.4363946263951765E-4 0.0 16-17 0.0 0.0 0.0 6.872789252790353E-4 0.0 18-19 0.0 0.0 0.0 9.163719003720469E-4 0.0 20-21 0.0 0.0 0.0 0.0013745578505580704 0.0 22-23 0.0 0.0 0.0 0.0027491157011161407 0.0 24-25 0.0 0.0 0.0 0.005040045452046258 0.0 26-27 0.0 0.0 0.0 0.010881916316918058 0.0 28-29 0.0 0.0 0.0 0.03585305060205634 0.0 30-31 0.0 0.0 0.0 0.08739896999798398 0.0 32-33 0.0 0.0 0.0 0.18018162491065376 0.0 34-35 0.0 0.0 0.0 0.3146592012902516 0.0 36-37 0.0 0.0 0.0 0.49781903487711454 0.0 38-39 0.0 0.0 0.0 0.7190083023294174 0.0 40-41 0.0 0.0 0.0 0.9507358466359987 0.0 42-43 0.0 0.0 0.0 1.18017246119165 0.0 44-45 0.0 0.0 0.0 1.4494712534134853 0.0 46-47 0.0 0.0 0.0 1.7834888110990965 0.0 48-49 0.0 0.0 0.0 2.122088228286568 0.0 50-51 0.0 0.0 0.0 2.4388092663526564 0.0 52-53 0.0 0.0 0.0 2.729184612283049 0.0 54-55 0.0 0.0 0.0 3.045676557374045 0.0 56-57 0.0 0.0 0.0 3.4252836171031653 0.0 58-59 0.0 0.0 0.0 3.8404000879717026 0.0 60-61 0.0 0.0 0.0 4.386443194105896 0.0 62-63 0.0 0.0 0.0 4.884033136007917 0.0 64-65 0.0 0.0 0.0 5.33076443743929 0.0 66-67 0.0 0.0 0.0 5.85332551362645 0.0 68-69 0.0 0.0 0.0 6.4102505360775615 0.0 70-71 0.0 0.0 0.0 7.047701739273867 0.0 72-73 0.0 0.0 0.0 7.713560471381705 0.0 74-75 0.0 0.0 0.0 8.43428697102432 0.0 76-77 0.0 0.0 0.0 9.09189835602881 0.0 78-79 0.0 0.0 0.0 9.926025878342466 0.0 80-81 0.0 0.0 0.0 10.803337426461155 0.0 82-83 0.0 0.0 0.0 11.562780638894488 0.0 84-85 0.0 0.0 0.0 12.551889558858566 0.0 86-87 0.0 0.0 0.0 13.607893627549805 0.0 88-89 0.0 0.0 0.0 14.575582354342686 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGC 20 0.0020988127 71.02987 8 GACTGGT 40 5.467249E-4 47.353245 7 TGGACTG 40 5.470341E-4 47.347816 5 GTATGGT 55 5.0495903E-5 43.048405 3 CGCAGAA 180 0.0 39.461037 9 TCCTTGC 30 0.0039236913 31.710623 94-95 CCATAGT 30 0.0039314404 31.697845 52-53 TTTGGAC 90 2.461057E-5 31.568829 3 GGACTGG 60 0.0040107965 31.565212 6 TCCTACA 65 0.005929121 29.140457 2 GGGATTC 65 0.005929121 29.140457 7 CATGGGG 310 0.0 29.022959 4 GCTTTGC 470 0.0 28.265535 1 TCTCGGG 60 5.32197E-6 27.746794 94-95 GTAAGCG 35 0.008333883 27.180532 94-95 CTGAACC 35 0.008338567 27.177404 86-87 TGTCATA 45 9.1088837E-4 26.307358 20-21 CTTTGCG 515 0.0 25.745451 2 CTTACTA 240 9.094947E-12 25.649675 9 GTTTTGG 95 0.0012335246 24.971432 1 >>END_MODULE