##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938942_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436504 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00778916115316 33.0 31.0 34.0 30.0 34.0 2 32.23052480618734 34.0 31.0 34.0 30.0 34.0 3 32.309987995528104 34.0 31.0 34.0 30.0 34.0 4 35.87394388138482 37.0 35.0 37.0 35.0 37.0 5 35.80081511280538 37.0 35.0 37.0 35.0 37.0 6 35.88333669336363 37.0 35.0 37.0 35.0 37.0 7 35.84057419863277 37.0 35.0 37.0 35.0 37.0 8 35.84451230687462 37.0 35.0 37.0 35.0 37.0 9 37.44690541209244 39.0 37.0 39.0 34.0 39.0 10-11 37.364270430511525 39.0 37.0 39.0 34.0 39.0 12-13 37.33269798214907 39.0 37.0 39.0 34.0 39.0 14-15 38.349802063669514 40.0 38.0 41.0 33.0 41.0 16-17 38.335167604420576 40.0 37.5 41.0 33.5 41.0 18-19 38.54489764125873 40.0 38.0 41.0 34.0 41.0 20-21 38.55336148122354 40.0 38.0 41.0 34.0 41.0 22-23 38.503712451661386 40.0 38.0 41.0 34.0 41.0 24-25 38.392280483111264 40.0 38.0 41.0 34.0 41.0 26-27 38.09815144878397 40.0 37.0 41.0 33.5 41.0 28-29 37.89583027876033 40.0 37.0 41.0 33.0 41.0 30-31 37.82140942580136 40.0 37.0 41.0 33.0 41.0 32-33 37.64491161593021 40.0 36.0 41.0 33.0 41.0 34-35 37.58055252643733 40.0 36.0 41.0 33.0 41.0 36-37 37.415854150248336 40.0 36.0 41.0 32.5 41.0 38-39 37.21136690614519 39.5 35.5 41.0 31.5 41.0 40-41 37.09859474369078 39.0 35.0 41.0 31.0 41.0 42-43 37.004884262228984 40.0 35.0 41.0 31.0 41.0 44-45 36.82971977347287 40.0 35.0 41.0 31.0 41.0 46-47 36.54108553415318 39.0 35.0 41.0 30.0 41.0 48-49 36.261259919725816 39.0 35.0 41.0 30.0 41.0 50-51 36.038492201675126 39.0 35.0 41.0 29.0 41.0 52-53 35.80527898942507 39.0 35.0 40.0 28.0 41.0 54-55 35.65342700181442 39.0 35.0 40.0 28.0 41.0 56-57 35.438997122592234 38.5 35.0 40.0 27.5 41.0 58-59 35.224601607316316 38.0 35.0 40.0 27.0 41.0 60-61 34.73497493722853 38.0 34.0 40.0 26.0 41.0 62-63 34.39725638253029 38.0 34.0 40.0 26.0 41.0 64-65 34.067826411670914 37.0 33.5 39.5 26.0 41.0 66-67 33.54973150303319 36.5 33.0 39.0 23.5 40.5 68-69 33.12517181973132 36.0 33.0 39.0 23.0 40.0 70-71 32.53427460000367 35.5 32.0 37.5 22.0 40.0 72-73 31.942355625607096 35.0 31.5 37.0 20.5 39.0 74-75 31.482663389109838 35.0 31.0 37.0 19.5 39.0 76-77 29.397170472664627 32.5 28.5 35.0 13.5 37.0 78-79 30.366494923299673 34.0 30.0 36.0 15.0 37.0 80-81 30.385834952257024 34.0 30.5 35.5 13.5 37.0 82-83 30.056272107472097 34.0 30.0 35.0 9.0 37.0 84-85 29.65080732364423 34.0 30.0 35.0 4.5 36.0 86-87 29.142234664516245 34.0 29.0 35.0 2.0 36.0 88-89 28.479503051518428 34.0 29.0 35.0 2.0 35.0 90-91 27.922638738705714 33.0 27.5 35.0 2.0 35.0 92-93 27.647012398511812 33.0 27.0 35.0 2.0 35.0 94-95 27.271015844070156 33.0 26.5 35.0 2.0 35.0 96-97 26.55832707145868 33.0 25.0 34.5 2.0 35.0 98-99 25.699009631068673 32.0 24.0 34.0 2.0 35.0 100-101 21.47768634422594 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19682798563376736 1101 2 0.1784485915268803 1101 3 0.0778031518182587 1101 4 0.10231941674388167 1101 5 0.08455857693516577 1101 6 0.15991976559908494 1101 7 0.2798810840646624 1101 8 0.05634590733042444 1101 9 0.0991852442302843 1101 10-11 0.19034435838490538 1101 12-13 0.32938789896333276 1101 14-15 0.302906436644065 1101 16-17 0.38997348751220784 1101 18-19 0.5406783165068063 1101 20-21 0.4384595513276537 1101 22-23 0.2995677334731752 1101 24-25 0.331154061044316 1101 26-27 0.4437859428620641 1101 28-29 0.3571051918856156 1101 30-31 0.25928490238911905 1101 32-33 0.4507698416750685 1101 34-35 0.7354162293572557 1101 36-37 0.7458908405152087 1101 38-39 0.634250425209558 1101 40-41 0.47284171889651105 1101 42-43 0.3997723883500939 1101 44-45 0.5349210916880622 1101 46-47 0.6664791170666291 1101 48-49 0.31160033235472895 1101 50-51 0.31836278809860374 1101 52-53 0.5393054926272569 1101 54-55 0.8673537480633726 1101 56-57 0.6957827388392346 1101 58-59 0.6650591748642825 1101 60-61 0.6020860076638428 1101 62-63 0.40648691525652936 1101 64-65 0.6603715428833041 1101 66-67 0.8731088747195273 1101 68-69 0.8028498127118837 1101 70-71 0.7470553867425309 1101 72-73 0.2974830578333467 1101 74-75 0.3767437268251186 1101 76-77 0.44980660245533244 1101 78-79 0.690139494189264 1101 80-81 0.9950245513227465 1101 82-83 0.8732998476806451 1101 84-85 0.6516632566739595 1101 86-87 0.8285879952111053 1101 88-89 0.910974176338879 1101 90-91 0.6938463798121539 1101 92-93 0.7574366094565761 1101 94-95 1.2232677525781028 1101 96-97 0.6244940612141008 1101 98-99 0.6283476275190516 1101 100-101 1.091852062095768 1102 1 0.2055210028114729 1102 2 0.2838948267623209 1102 3 0.1504280389731747 1102 4 0.2659716203006326 1102 5 0.10929295696584518 1102 6 0.21561315524448332 1102 7 0.23271657329151196 1102 8 0.1793801932972272 1102 9 0.15998586038023177 1102 10-11 0.2650620089344571 1102 12-13 0.29764607589180514 1102 14-15 0.34294671365901763 1102 16-17 0.4291015343295044 1102 18-19 0.14953357808645507 1102 20-21 0.238390739499593 1102 22-23 0.3100946805638145 1102 24-25 0.21782603064085038 1102 26-27 0.2615703282588058 1102 28-29 0.2741553740160114 1102 30-31 0.5809738855523179 1102 32-33 0.3267286303343724 1102 34-35 0.4815858094788439 1102 36-37 0.6954340941152068 1102 38-39 0.4411196132931181 1102 40-41 0.6448018459143086 1102 42-43 0.5254707652747683 1102 44-45 0.6466203650604072 1102 46-47 0.9133652240216747 1102 48-49 0.9068226450634569 1102 50-51 0.9174612900209169 1102 52-53 1.078102106327755 1102 54-55 0.8628462576749314 1102 56-57 0.7724605795366912 1102 58-59 0.7934405817820718 1102 60-61 0.9052589273686493 1102 62-63 1.1062527025206634 1102 64-65 0.8748024564392338 1102 66-67 0.911993870584503 1102 68-69 0.8167731601232333 1102 70-71 0.86605250169616 1102 72-73 0.9377455528618484 1102 74-75 0.8687514539514964 1102 76-77 0.9179290496312049 1102 78-79 1.08507203965873 1102 80-81 0.9986829416310137 1102 82-83 0.7073181427886901 1102 84-85 0.5704589865528149 1102 86-87 0.7986138154291531 1102 88-89 0.6625855725876875 1102 90-91 0.8282567129497842 1102 92-93 0.8680910793845484 1102 94-95 1.0084012412115229 1102 96-97 0.506241881646794 1102 98-99 0.7299018910115329 1102 100-101 1.098887913689488 1103 1 0.14687383195688852 1103 2 0.14493187290104714 1103 3 0.156885002787142 1103 4 0.022235059509334576 1103 5 -0.03009390793554445 1103 6 0.04868436001194709 1103 7 0.044475496926089875 1103 8 0.1413572160901282 1103 9 0.11318976773416978 1103 10-11 0.23693950478749315 1103 12-13 0.08989587622355089 1103 14-15 0.26954559551685264 1103 16-17 0.0937879015962011 1103 18-19 0.16095767343572476 1103 20-21 0.31797235773392174 1103 22-23 0.29533458963797443 1103 24-25 0.16091443759545143 1103 26-27 0.1355366611211224 1103 28-29 0.24703428290585094 1103 30-31 0.3893875981529149 1103 32-33 0.43392590255898256 1103 34-35 0.3817301849780179 1103 36-37 -0.004298657620374513 1103 38-39 0.1573019564767506 1103 40-41 -0.050267884379969985 1103 42-43 -0.05501396778414147 1103 44-45 -0.2995797946741874 1103 46-47 0.12909908161213934 1103 48-49 -0.10100435008307329 1103 50-51 0.1668223516416063 1103 52-53 0.2930557218588703 1103 54-55 0.4023344926076575 1103 56-57 0.10206062868579835 1103 58-59 0.16549624314473732 1103 60-61 0.5039687633335035 1103 62-63 0.24688058235109622 1103 64-65 0.1751034947141008 1103 66-67 0.4202908540581163 1103 68-69 0.8219921808272943 1103 70-71 0.4651524586906959 1103 72-73 0.7383046239437405 1103 74-75 0.48760013614109 1103 76-77 0.42628132584918177 1103 78-79 0.3557853160610165 1103 80-81 0.17249261171763663 1103 82-83 0.38042436629425325 1103 84-85 0.3670523391362117 1103 86-87 0.2760236575376531 1103 88-89 0.3685510453406522 1103 90-91 0.6365659836999242 1103 92-93 0.24618286534018807 1103 94-95 -0.3793882777416009 1103 96-97 -0.2865946361390357 1103 98-99 -0.14114928549519234 1103 100-101 0.2635282036692175 1104 1 -0.03368641919017534 1104 2 -0.007579597758216039 1104 3 0.04295117037951712 1104 4 0.10745602062983295 1104 5 0.09805146757761207 1104 6 0.09488093088854299 1104 7 0.0752047893675254 1104 8 0.0771094498500915 1104 9 0.21838175034706353 1104 10-11 -0.03343600837357741 1104 12-13 0.28236750143486233 1104 14-15 0.3351094689773717 1104 16-17 0.1911255012098252 1104 18-19 -0.1524871466560498 1104 20-21 0.02588107543495255 1104 22-23 0.21244398350419402 1104 24-25 -0.0234794286204405 1104 26-27 0.0046745505198231285 1104 28-29 -0.03997972337282363 1104 30-31 -0.11454261194126758 1104 32-33 -0.22531703392978386 1104 34-35 -0.03271077131274325 1104 36-37 -0.436464801063984 1104 38-39 -0.08250123115908536 1104 40-41 -0.03995636542319403 1104 42-43 -0.23699899555521853 1104 44-45 -0.08664209846553916 1104 46-47 -0.26491806400485984 1104 48-49 -0.3214643838913247 1104 50-51 -0.37620469496548736 1104 52-53 -0.5739510397929024 1104 54-55 -0.6178599048808735 1104 56-57 -0.7265013128742126 1104 58-59 -0.6156798012889766 1104 60-61 -0.7460553854228351 1104 62-63 -0.8262297774653717 1104 64-65 -0.8237192534143674 1104 66-67 -0.7279885470527248 1104 68-69 -0.6255386088370329 1104 70-71 -0.5706541097710343 1104 72-73 -0.6114538884928677 1104 74-75 -0.5145128800385734 1104 76-77 -0.6321526273022329 1104 78-79 -0.6792183062524337 1104 80-81 -0.48735404367961266 1104 82-83 -0.3686881669102924 1104 84-85 -0.26270496586257863 1104 86-87 -0.44059672401269623 1104 88-89 -0.304927583009988 1104 90-91 -0.2811592034529369 1104 92-93 -0.3250499286709214 1104 94-95 -0.24330399858197893 1104 96-97 -0.09551760160631773 1104 98-99 -0.5054212004915719 1104 100-101 0.3313741346037702 1105 1 0.15350706651353363 1105 2 0.23335220570250215 1105 3 0.1477443599788444 1105 4 -0.048530839738447185 1105 5 0.09300843991036345 1105 6 0.23328659388474904 1105 7 -0.021514929333314115 1105 8 0.04759154478855265 1105 9 0.16577695715746188 1105 10-11 0.1836570794398753 1105 12-13 0.26565379735416883 1105 14-15 0.44953761739968456 1105 16-17 0.34385058680249614 1105 18-19 0.3546378649105364 1105 20-21 0.4301560823749 1105 22-23 0.23683789485794193 1105 24-25 0.5569082830479246 1105 26-27 0.4628845070294929 1105 28-29 0.33198983339593724 1105 30-31 0.6353301557093332 1105 32-33 0.17575403112064691 1105 34-35 0.2808013041938793 1105 36-37 0.44029331867321986 1105 38-39 0.21954836632402674 1105 40-41 0.4123546367050537 1105 42-43 0.43696232581056904 1105 44-45 0.421026542230301 1105 46-47 0.37454015751822567 1105 48-49 0.26511144658891794 1105 50-51 0.620440997641154 1105 52-53 0.696822532891467 1105 54-55 0.7457354499817157 1105 56-57 0.7193902388977875 1105 58-59 0.5692003689710461 1105 60-61 0.6091773341107967 1105 62-63 0.5777444984668918 1105 64-65 0.44980716464799997 1105 66-67 0.47219420486732844 1105 68-69 0.622009276792653 1105 70-71 0.7242589095682845 1105 72-73 0.7883217199090922 1105 74-75 0.7213874623321104 1105 76-77 0.6583419595396336 1105 78-79 0.5273376297149959 1105 80-81 0.655516318122924 1105 82-83 1.1033785491071058 1105 84-85 0.6959445205813957 1105 86-87 0.6359393174419132 1105 88-89 1.0922802452494729 1105 90-91 1.0881616071327898 1105 92-93 0.868458908194075 1105 94-95 0.8894571747316142 1105 96-97 0.792645163268304 1105 98-99 0.8705272588403439 1105 100-101 2.143833651583506 1106 1 -0.05524194823705386 1106 2 -0.2068459701785912 1106 3 0.001522087712210407 1106 4 0.0016943601264358676 1106 5 0.07598896012205358 1106 6 0.015625294481530716 1106 7 0.040831653258734946 1106 8 0.07379917906397537 1106 9 -0.13720694105219877 1106 10-11 -0.01400084196536966 1106 12-13 -0.05178793218078681 1106 14-15 -0.027618671306541387 1106 16-17 0.166429688255036 1106 18-19 0.12086070017682715 1106 20-21 0.041877735341905975 1106 22-23 0.14792054320835035 1106 24-25 -0.04130419302459387 1106 26-27 0.16599508016314957 1106 28-29 0.15577656912389415 1106 30-31 0.21064825051848146 1106 32-33 0.2627093289474658 1106 34-35 0.349067828233963 1106 36-37 0.3585429527234396 1106 38-39 0.3978250243580348 1106 40-41 0.6002022889964636 1106 42-43 0.6076807133795299 1106 44-45 0.774061110409562 1106 46-47 0.757779657574531 1106 48-49 0.5021399343718258 1106 50-51 0.712972807122064 1106 52-53 0.664081287536213 1106 54-55 0.7307652889638163 1106 56-57 0.7402916013569723 1106 58-59 0.8247233956572231 1106 60-61 0.8674421691519001 1106 62-63 0.6559901915194786 1106 64-65 0.7735460454046645 1106 66-67 0.8116661111841807 1106 68-69 1.0396677611975562 1106 70-71 0.9905858997720856 1106 72-73 0.9638115296152066 1106 74-75 1.1620050729615414 1106 76-77 0.4913562597310488 1106 78-79 0.8339810257289564 1106 80-81 1.0971473281443203 1106 82-83 1.1303068229501427 1106 84-85 0.8285422010227848 1106 86-87 1.2507518385544003 1106 88-89 1.4540638281793257 1106 90-91 1.517009709823931 1106 92-93 1.3431267383933765 1106 94-95 1.3399329094461763 1106 96-97 0.9138167655653433 1106 98-99 1.0246456013693397 1106 100-101 1.8946331111038326 1107 1 0.049835504430760125 1107 2 0.02356137348176901 1107 3 0.004620194424774127 1107 4 -0.20407156069972388 1107 5 -0.23473966867886986 1107 6 -0.07855141234577445 1107 7 -0.15082067032130198 1107 8 -0.08024557136631927 1107 9 0.030262938465874356 1107 10-11 0.017224057633022483 1107 12-13 0.024187397710200287 1107 14-15 -0.056993312729375134 1107 16-17 0.21234190856483792 1107 18-19 0.23290889294791128 1107 20-21 -0.1276432055645742 1107 22-23 0.10044492645562997 1107 24-25 -0.05299380862987135 1107 26-27 -0.003081225057734116 1107 28-29 0.138120229479604 1107 30-31 0.06949793497057044 1107 32-33 -0.1675258976733005 1107 34-35 -0.08757875811141247 1107 36-37 0.021078810440023688 1107 38-39 -0.20785483794833937 1107 40-41 -0.28267206556463265 1107 42-43 -0.05254590738925202 1107 44-45 0.10071056537227463 1107 46-47 0.16553926744256842 1107 48-49 -0.03764778794601398 1107 50-51 -0.14435651014700568 1107 52-53 -0.07967946977876039 1107 54-55 -0.06529036221231621 1107 56-57 0.015707550869308307 1107 58-59 -0.0876977173662965 1107 60-61 -0.32297664897775746 1107 62-63 0.10535909570766222 1107 64-65 -0.16845051225453744 1107 66-67 0.07348481456914868 1107 68-69 0.10573201822566602 1107 70-71 0.15112806844678772 1107 72-73 0.37323493753259385 1107 74-75 0.04297179700055764 1107 76-77 -0.4117848762414127 1107 78-79 0.11864826611909152 1107 80-81 0.39264359894179535 1107 82-83 0.07781112587613137 1107 84-85 0.38618706619777043 1107 86-87 0.12489125335646634 1107 88-89 0.39944535428374195 1107 90-91 0.3764904900878463 1107 92-93 0.6258576620881584 1107 94-95 0.5695083540818615 1107 96-97 0.4523136100862821 1107 98-99 0.4364929909275723 1107 100-101 1.0011879016947667 1108 1 -0.1698842494995212 1108 2 -0.18325111227808577 1108 3 -0.055940297714663245 1108 4 0.057548398288652436 1108 5 -0.06923499791103893 1108 6 -0.12009846178756334 1108 7 -0.03499702028712193 1108 8 -0.08127086186529198 1108 9 0.15227250784386825 1108 10-11 0.028747183629832307 1108 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-1.7724991619556931 2316 78-79 -1.7692088767380518 2316 80-81 -2.2087849724867787 2316 82-83 -2.134569826504819 2316 84-85 -2.478812933802228 2316 86-87 -2.3186801752149613 2316 88-89 -2.9005546457162588 2316 90-91 -2.776128557531205 2316 92-93 -3.140809004578511 2316 94-95 -3.2433487887752825 2316 96-97 -3.459114961342287 2316 98-99 -3.7373165328819518 2316 100-101 -4.190954955448088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 26.0 6 106.0 7 132.0 8 179.0 9 359.0 10 489.0 11 742.0 12 978.0 13 1384.0 14 1658.0 15 2163.0 16 2892.0 17 3807.0 18 3795.0 19 2650.0 20 2678.0 21 2898.0 22 3154.0 23 3508.0 24 4168.0 25 4852.0 26 5423.0 27 6476.0 28 7966.0 29 9657.0 30 11547.0 31 13727.0 32 16964.0 33 21542.0 34 29706.0 35 43393.0 36 60263.0 37 92249.0 38 69903.0 39 5061.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63060474809255 21.867172099378255 16.61349348925687 17.888729663272322 2 13.66012682587101 26.160814104796295 37.934131187801256 22.24492788153144 3 22.16634899107454 28.999276066198703 32.85307809321335 15.981296849513408 4 11.148809632901417 19.32926158752268 41.55334200832066 27.968586771255243 5 9.76398733568231 42.189955601578 36.379214757320696 11.666842305418989 6 23.64147865769844 40.6275772959698 18.1844839909829 17.546460055348863 7 21.8332478052893 37.459450543408536 23.474698971830726 17.232602679471437 8 21.15009255356194 32.29775672158789 22.563367116910726 23.988783607939446 9 22.108846654326193 22.652255191246816 25.307671865549914 29.931226288877077 10-11 21.955927093451606 26.66940050950278 29.018634422594065 22.35603797445155 12-13 28.446245624324174 25.743406704176824 27.518762714660117 18.291584956838882 14-15 23.834195110664123 28.489510906046036 30.176677716660443 17.4996162666294 16-17 17.077076572557043 31.45778469144514 31.97027683052135 19.494861905476473 18-19 16.402713377197 34.132104173157636 31.82868885508495 17.636493594560417 20-21 19.288253944981033 30.560659237945124 28.89893334310797 21.252153473965873 22-23 18.128241665597567 29.55608654216227 29.524815351062074 22.790856441178086 24-25 21.149061635174018 30.21014698605282 29.722637135054892 18.91815424371827 26-27 21.204616681634075 27.167448637354987 33.66887817751956 17.95905650349138 28-29 17.882653996297858 30.145542767076588 33.2514707769001 18.720332459725455 30-31 18.223315250261166 30.92079339479134 31.32342429851731 19.532467056430182 32-33 18.03992632369921 31.618496050437113 31.188259443212434 19.153318182651248 34-35 20.231045029152682 28.660122109073416 30.849265169131375 20.259567692642527 36-37 20.5743676686217 27.9480159457478 32.86450696480939 18.613109420821115 38-39 20.963981849445712 28.143211126780088 33.03151144261623 17.861295581157975 40-41 18.457307217812744 29.655793345422016 33.8474649854008 18.039434451364443 42-43 17.369296430952364 31.12475744725695 30.780659010361898 20.725287111428788 44-45 18.422210156631223 30.41468214422352 30.017663108844612 21.145444590300638 46-47 19.829875166951886 30.56038561017908 29.03954841089294 20.57019081197609 48-49 19.84950813512882 29.372374009741076 30.298257510847606 20.4798603442825 50-51 18.997736551035278 30.3594256154611 31.94051802740881 18.702319806094817 52-53 18.659393728350715 31.29982772208273 30.92193885966681 19.11883968989975 54-55 16.946007367630077 29.11324982130748 32.502336748345954 21.438406062716492 56-57 19.789394827996993 30.55882649414438 29.054716566171212 20.597062111687407 58-59 20.284740311063153 29.11331651787044 29.897847208381158 20.70409596268525 60-61 18.941438448744325 29.52178475044559 31.598999308129706 19.93777749268038 62-63 20.536810659237943 29.221839891501567 30.97325568608764 19.268093763172846 64-65 19.369282685342366 30.641026522154487 30.885812926829825 19.103877865673315 66-67 18.555246147449065 29.306993297769857 30.30253392638887 21.83522662839221 68-69 17.304108270952295 30.951036374778777 30.981964386966705 20.76289096730222 70-71 19.937617982882173 30.33133716987702 28.603976137675712 21.127068709565087 72-73 18.627664351300332 28.169844949874456 30.520682513791396 22.681808185033812 74-75 17.738554514964353 32.079431116324244 29.17155398346865 21.010460385242745 76-77 19.695803710589182 27.932407960666882 30.20998076695908 22.161807561784855 78-79 18.358021919615858 28.038803768121255 32.07988930227443 21.523285009988456 80-81 19.20898777559885 30.408541502483367 29.805110606088377 20.577360115829407 82-83 18.347483642761578 29.079229514506167 30.88803309935304 21.685253743379214 84-85 17.83007715851401 28.465947620182174 30.506364202848086 23.19761101845573 86-87 18.634253550465882 29.717809510809406 30.230205313912688 21.417731624812024 88-89 19.578362458727838 27.824626393474684 29.96537165077137 22.631639497026104 90-91 18.589790005160513 26.53559660210857 31.59864332598909 23.275970066741834 92-93 18.702005021718012 29.8598638271356 30.520109781353668 20.918021369792715 94-95 18.06879203856093 28.82161446401408 30.35207008412294 22.757523413302057 96-97 16.35477460037457 28.27532488359173 30.639572511039454 24.730328004994245 98-99 18.088264941443835 28.798017887579498 28.97842860546524 24.135288565511427 100-101 18.650501829311597 27.61905416457619 30.24813116977432 23.482312836337897 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5697.0 1 3459.5 2 1106.0 3 936.0 4 691.5 5 608.5 6 1081.0 7 2046.5 8 2673.5 9 2662.0 10 2028.5 11 1250.0 12 1058.5 13 1001.5 14 909.5 15 818.0 16 749.0 17 689.0 18 678.0 19 713.0 20 779.5 21 859.0 22 933.5 23 1070.0 24 1168.5 25 1399.0 26 1928.5 27 2451.5 28 2700.5 29 3320.0 30 4662.0 31 5848.0 32 8009.5 33 10946.5 34 12898.0 35 14393.5 36 16641.5 37 18058.5 38 17406.5 39 16139.5 40 14746.0 41 13292.0 42 11292.5 43 11128.0 44 12590.0 45 12855.5 46 13329.5 47 22180.0 48 53895.5 49 53527.0 50 22660.5 51 10997.5 52 4321.5 53 1848.0 54 1275.5 55 1099.0 56 1004.0 57 891.0 58 813.0 59 691.5 60 547.5 61 430.5 62 319.0 63 266.5 64 215.5 65 145.5 66 115.0 67 92.0 68 63.0 69 45.5 70 35.0 71 26.5 72 30.5 73 36.5 74 24.0 75 12.0 76 10.5 77 8.5 78 6.0 79 5.0 80 4.0 81 3.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4034327291387937 2 0.0 3 0.0 4 0.0 5 4.581859501860235E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.872789252790352E-4 16-17 0.002176383263383612 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020618367758371055 36-37 0.0054982314022322815 38-39 0.009965544416546011 40-41 0.001718197313197588 42-43 2.2909297509301175E-4 44-45 2.2909297509301175E-4 46-47 2.2909297509301175E-4 48-49 4.581859501860235E-4 50-51 4.581859501860235E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.2909297509301175E-4 60-61 0.0013745578505580704 62-63 0.0 64-65 2.2909297509301175E-4 66-67 0.001718197313197588 68-69 0.0014891043381045764 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12267928816230779 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.13986126129428367 88-89 0.4327566299506992 90-91 0.3366521268991807 92-93 0.0 94-95 0.0 96-97 3.436394626395176E-4 98-99 0.0 100-101 2.2909297509301175E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 436504.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.90748309293844 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45512918849029 15.148543885050309 2 10.657228638425632 4.456316551509264 3 4.347921369244593 2.727122775507212 4 2.3931099471850277 2.0013562304125503 5 1.5230873748109837 1.5921961768964317 6 1.1045122833161665 1.385554313362535 7 0.8459161534921436 1.2380184374026355 8 0.6486818171856852 1.0849843300405035 9 0.5423944248427603 1.0206092040393673 >10 4.507900330915386 19.461906420101535 >50 0.6103306962372071 8.867272693950113 >100 0.29694725077250117 11.093598225904001 >500 0.02191492625627315 3.148195663728168 >1k 0.039446867261291665 18.483679416454375 >5k 0.0054787315640682874 8.290645675641002 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9782 2.2409874823598406 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8613 1.9731777944761102 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7354 1.6847497388340085 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5428 1.2435166688048678 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5012 1.1482139911661748 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4811 1.1021663031724795 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4364 0.9997617433059034 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3609 0.8267965471106795 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3414 0.7821234169675421 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3129 0.7168319190660337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3012 0.6900280409801514 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2961 0.6783442992504077 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2921 0.6691805802466873 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2808 0.643293074061177 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2723 0.6238201711782709 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2700 0.6185510327511318 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2666 0.6107618715979694 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2615 0.5990781298682257 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2577 0.5903725968146912 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2558 0.586019830287924 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2275 0.5211865183366017 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2199 0.5037754522295329 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2196 0.5030881733042538 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2106 0.4824698055458827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2041 0.46757876216483696 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2034 0.46597511133918584 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1867 0.4277165844986529 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1703 0.390145336583399 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1588 0.36379964444770263 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1534 0.35142862379268003 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1534 0.35142862379268003 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1532 0.350970437842494 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1471 0.33699576636182027 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1415 0.3241665597566116 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1401 0.32095925810530945 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1323 0.3030900060480545 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1301 0.29804996059600825 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 1218 0.2790352436632883 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1045 0.23940215897219727 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1021 0.233903927569965 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1010 0.23138390484394186 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 954 0.21855469823873322 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 934 0.21397283873687295 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 887 0.2032054689075014 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 883 0.20228909700712935 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 850 0.19472902882905999 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 837 0.19175082015285083 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 766 0.175485218921247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 740 0.16952880156882869 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 687 0.15738687388889908 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 681 0.156012316038341 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 615 0.14089217968220222 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 588 0.1347066693546909 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 574 0.13149936770338874 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 569 0.1303539028279237 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 556 0.12737569415171454 No Hit GCTTTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 556 0.12737569415171454 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 531 0.12164836977438924 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 519 0.11889925407327309 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 513 0.11752469622271502 No Hit CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC 502 0.1150046734966919 No Hit TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC 497 0.11385920862122684 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 497 0.11385920862122684 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 481 0.11019372101973865 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 471 0.10790279126880853 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 461 0.10561186151787842 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 455 0.10423730366732034 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 451 0.10332093176694829 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 449 0.10286274581676229 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 448 0.10263365284166927 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1454648754650588E-4 0.0 14-15 0.0 0.0 0.0 3.4363946263951765E-4 0.0 16-17 0.0 0.0 0.0 4.581859501860235E-4 0.0 18-19 0.0 0.0 0.0 6.872789252790353E-4 0.0 20-21 0.0 0.0 0.0 0.0011454648754650589 0.0 22-23 0.0 0.0 0.0 0.002405476238476623 0.0 24-25 0.0 0.0 0.0 0.0045818595018602346 0.0 26-27 0.0 0.0 0.0 0.010194637391639023 0.0 28-29 0.0 0.0 0.0 0.03539486465187032 0.0 30-31 0.0 0.0 0.0 0.0877426094606235 0.0 32-33 0.0 0.0 0.0 0.18235800817403736 0.0 34-35 0.0 0.0 0.0 0.3198137932298444 0.0 36-37 0.0 0.0 0.0 0.5048063706174514 0.0 38-39 0.0 0.0 0.0 0.7312647764968935 0.0 40-41 0.0 0.0 0.0 0.9683760057181607 0.0 42-43 0.0 0.0 0.0 1.2018217473379398 0.0 44-45 0.0 0.0 0.0 1.4760460385242746 0.0 46-47 0.0 0.0 0.0 1.8147600021992925 0.0 48-49 0.0 0.0 0.0 2.1593158367391823 0.0 50-51 0.0 0.0 0.0 2.4779841650935612 0.0 52-53 0.0 0.0 0.0 2.7723686380880816 0.0 54-55 0.0 0.0 0.0 3.0920678848303798 0.0 56-57 0.0 0.0 0.0 3.474653153235709 0.0 58-59 0.0 0.0 0.0 3.8922896468302697 0.0 60-61 0.0 0.0 0.0 4.444632809779521 0.0 62-63 0.0 0.0 0.0 4.9450864138702055 0.0 64-65 0.0 0.0 0.0 5.393535912614775 0.0 66-67 0.0 0.0 0.0 5.918273372065319 0.0 68-69 0.0 0.0 0.0 6.479092975093012 0.0 70-71 0.0 0.0 0.0 7.11677327126441 0.0 72-73 0.0 0.0 0.0 7.785037479610725 0.0 74-75 0.0 0.0 0.0 8.514469512306874 0.0 76-77 0.0 0.0 0.0 9.176891849788317 0.0 78-79 0.0 0.0 0.0 10.01605941755402 0.0 80-81 0.0 0.0 0.0 10.898754650587394 0.0 82-83 0.0 0.0 0.0 11.658656048970915 0.0 84-85 0.0 0.0 0.0 12.6524613749244 0.0 86-87 0.0 0.0 0.0 13.717629162619357 0.0 88-89 0.0 0.0 0.0 14.690014295401646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 75 3.6379788E-11 57.134342 1 GGATGCC 40 5.41345E-4 47.448338 8 GGGATGC 40 5.41345E-4 47.448338 7 CGCAGAA 135 3.110472E-10 35.14692 9 CTTTGCG 455 0.0 34.413082 2 GCTTTGC 465 0.0 33.789127 1 GGATATG 100 1.273098E-6 33.32837 1 TTGCGTT 490 0.0 31.955004 4 TGTAGGA 90 2.4275476E-5 31.632229 2 CCTACAG 60 0.003969379 31.632225 3 TTTGCGT 515 0.0 30.40379 3 GCGTTGT 40 4.5214873E-4 29.65521 94-95 AGACCAG 80 4.517824E-4 29.65521 5 GGTGGTA 145 2.4183464E-8 29.450693 5 GTCTAGA 65 0.0057733334 29.299665 1 CTGTAGG 100 4.86739E-5 28.567173 1 GTAGATA 120 5.417298E-6 27.678196 6 GATTGGC 120 5.417298E-6 27.678196 9 GGTACTC 175 5.000402E-9 27.113337 8 ACCAGGC 70 0.008430596 27.113337 7 >>END_MODULE