FastQCFastQC Report
Fri 27 May 2016
SRR938941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770576
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT148930.5375416519886118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107000.3862012808888838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83790.3024280871558838No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77770.2806997534086775No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62120.22421330438147155No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56940.2055168311571312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.19873123855833588No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41810.15090724816789No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36630.13221077494354963No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35270.12730204838271897No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34180.12336784841852382No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29820.10763104856174313No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29600.10683698985337343No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29530.10658433480980128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122050.024.3081931
TACATGG120900.023.623442
ACATGGG121500.022.4542393
GTAAGCG3000.021.4038994-95
GAGTACT97750.020.92371212-13
TTTGCGT6050.020.3549543
GTTCGCG1656.490896E-520.0845587
CATGGGG46550.019.942914
AGTACTT103950.019.58464212-13
TCGCGCA1708.165154E-519.4938379
CGTTCGC1751.0200414E-418.936876
GTACTTT108400.018.86852514-15
CATGGGA78000.018.5814234
AGAGTAC176400.018.41067910-11
TTCGCGC1851.5615989E-417.9132548
GTGTAGC15400.017.5697081
CTTTGCG7300.016.8695162
AGTACAT92850.016.8338782
CCTATAC7650.016.716853
ACTTTTT130750.016.18855916-17
Produced by FastQC (version 0.11.5)